Relevant bibliographies by topics / Genetically engineered feedstocks

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  2. Dissertations / Theses

Academic literature on the topic 'Genetically engineered feedstocks'

Author: Grafiati
Published: 4 June 2021
Last updated: 14 February 2022

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Journal articles on the topic "Genetically engineered feedstocks"

1

Endres, A. Bryan. "New hope for dedicated genetically engineered bioenergy feedstocks?" GCB Bioenergy 4, no. 2 (October 17, 2011): 127–29. http://dx.doi.org/10.1111/j.1757-1707.2011.01134.x.

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Heinrich, Daniel, Matthias Raberg, Philipp Fricke, Shane T. Kenny, Laura Morales-Gamez, Ramesh P. Babu, Kevin E. O'Connor, and Alexander Steinbüchel. "Synthesis Gas (Syngas)-Derived Medium-Chain-Length Polyhydroxyalkanoate Synthesis in Engineered Rhodospirillum rubrum." Applied and Environmental Microbiology 82, no. 20 (August 12, 2016): 6132–40. http://dx.doi.org/10.1128/aem.01744-16.

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ABSTRACTThe purple nonsulfur alphaproteobacteriumRhodospirillum rubrumS1 was genetically engineered to synthesize a heteropolymer of mainly 3-hydroxydecanoic acid and 3-hydroxyoctanoic acid [P(3HD-co-3HO)] from CO- and CO2-containing artificial synthesis gas (syngas). For this, genes fromPseudomonas putidaKT2440 coding for a 3-hydroxyacyl acyl carrier protein (ACP) thioesterase (phaG), a medium-chain-length (MCL) fatty acid coenzyme A (CoA) ligase (PP_0763), and an MCL polyhydroxyalkanoate (PHA) synthase (phaC1) were cloned and expressed under the control of the CO-inducible promoter PcooFfromR. rubrumS1 in a PHA-negative mutant ofR. rubrum. P(3HD-co-3HO) was accumulated to up to 7.1% (wt/wt) of the cell dry weight by a recombinant mutant strain utilizing exclusively the provided gaseous feedstock syngas. In addition to an increased synthesis of these medium-chain-length PHAs (PHAMCL), enhanced gene expression through the PcooFpromoter also led to an increased molar fraction of 3HO in the synthesized copolymer compared with the Placpromoter, which regulated expression on the original vector. The recombinant strains were able to partially degrade the polymer, and the deletion ofphaZ2, which codes for a PHA depolymerase most likely involved in intracellular PHA degradation, did not reduce mobilization of the accumulated polymer significantly. However, an amino acid exchange in the active site of PhaZ2 led to a slight increase in PHAMCLaccumulation. The accumulated polymer was isolated; it exhibited a molecular mass of 124.3 kDa and a melting point of 49.6°C. With the metabolically engineered strains presented in this proof-of-principle study, we demonstrated the synthesis of elastomeric second-generation biopolymers from renewable feedstocks not competing with human nutrition.IMPORTANCEPolyhydroxyalkanoates (PHAs) are natural biodegradable polymers (biopolymers) showing properties similar to those of commonly produced petroleum-based nondegradable polymers. The utilization of cheap substrates for the microbial production of PHAs is crucial to lower production costs. Feedstock not competing with human nutrition is highly favorable. Syngas, a mixture of carbon monoxide, carbon dioxide, and hydrogen, can be obtained by pyrolysis of organic waste and can be utilized for PHA synthesis by several kinds of bacteria. Up to now, the biosynthesis of PHAs from syngas has been limited to short-chain-length PHAs, which results in a stiff and brittle material. In this study, the syngas-utilizing bacteriumRhodospirillum rubrumwas genetically modified to synthesize a polymer which consisted of medium-chain-length constituents, resulting in a rubber-like material. This study reports the establishment of a microbial synthesis of these so-called medium-chain-length PHAs from syngas and therefore potentially extends the applications of syngas-derived PHAs.
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Manandhar, Ashish, and Ajay Shah. "Techno-Economic Analysis of Bio-Based Lactic Acid Production Utilizing Corn Grain as Feedstock." Processes 8, no. 2 (February 6, 2020): 199. http://dx.doi.org/10.3390/pr8020199.

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Lactic acid is an important chemical with numerous commercial applications that can be fermentatively produced from biological feedstocks. Producing lactic acid from corn grain could complement the use of already existing infrastructure for corn grain-based ethanol production with a higher value product. The objective of this study was to evaluate the techno-economic feasibility of producing 100,000 metric tons (t) of lactic acid annually from corn grain in a biorefinery. The study estimated the resources (equipment, raw materials, energy, and labor) requirements and costs to produce lactic acid from bacteria, fungi and yeast-based fermentation pathways. Lactic acid production costs were $1181, $1251 and $844, for bacteria, fungi and yeast, respectively. Genetically engineered yeast strains capable of producing lactic acid at low pH support significantly cheaper processes because they do not require simultaneous neutralization and recovery of lactic acid, resulting in lower requirements for chemical, equipment, and utilities. Lactic acid production costs were highly sensitive to sugar-to-lactic-acid conversion rates, grain price, plant size, annual operation hours, and potential use of gypsum. Improvements in process efficiencies and lower equipment and chemical costs would further reduce the cost of lactic acid production from corn grain.
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Sato, Trey K., Tongjun Liu, Lucas S. Parreiras, Daniel L. Williams, Dana J. Wohlbach, Benjamin D. Bice, Irene M. Ong, et al. "Harnessing Genetic Diversity in Saccharomyces cerevisiae for Fermentation of Xylose in Hydrolysates of Alkaline Hydrogen Peroxide-Pretreated Biomass." Applied and Environmental Microbiology 80, no. 2 (November 8, 2013): 540–54. http://dx.doi.org/10.1128/aem.01885-13.

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ABSTRACTThe fermentation of lignocellulose-derived sugars, particularly xylose, into ethanol by the yeastSaccharomyces cerevisiaeis known to be inhibited by compounds produced during feedstock pretreatment. We devised a strategy that combined chemical profiling of pretreated feedstocks, high-throughput phenotyping of genetically diverseS. cerevisiaestrains isolated from a range of ecological niches, and directed engineering and evolution against identified inhibitors to produce strains with improved fermentation properties. We identified and quantified for the first time the major inhibitory compounds in alkaline hydrogen peroxide (AHP)-pretreated lignocellulosic hydrolysates, including Na+, acetate, andp-coumaric (pCA) and ferulic (FA) acids. By phenotyping these yeast strains for their abilities to grow in the presence of these AHP inhibitors, one heterozygous diploid strain tolerant to all four inhibitors was selected, engineered for xylose metabolism, and then allowed to evolve on xylose with increasing amounts ofpCA and FA. After only 149 generations, one evolved isolate, GLBRCY87, exhibited faster xylose uptake rates in both laboratory media and AHP switchgrass hydrolysate than its ancestral GLBRCY73 strain and completely converted 115 g/liter of total sugars in undetoxified AHP hydrolysate into more than 40 g/liter ethanol. Strikingly, genome sequencing revealed that during the evolution from GLBRCY73, the GLBRCY87 strain acquired the conversion of heterozygous to homozygous alleles in chromosome VII and amplification of chromosome XIV. Our approach highlights that simultaneous selection on xylose andpCA or FA with a wildS. cerevisiaestrain containing inherent tolerance to AHP pretreatment inhibitors has potential for rapid evolution of robust properties in lignocellulosic biofuel production.
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Li, Yan-Feng, Hong Jiang, Zhong Hu, Guang-Lei Liu, Zhen-Ming Chi, and Zhe Chi. "Overexpression of an Inulinase Gene in an Oleaginous Yeast, Aureobasidium melanogenum P10, for Efficient Lipid Production from Inulin." Journal of Molecular Microbiology and Biotechnology 28, no. 4 (2018): 190–200. http://dx.doi.org/10.1159/000493139.

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In this study, in order to directly and efficiently convert inulin into a single-cell oil (SCO), an <i>INU1</i> gene encoding inulinase from<b><i></i></b> <i>Kluyveromyces marxianus</i> was integrated into the genomic DNA and actively expressed in an SCO producer <i>Aureobasidium</i> <i>melanogenum</i> P10. The transformant API41 obtained produced 28.5 U/mL of inulinase and its wild-type strain P10 yielded only 8.62 U/mL. Most (97.5%) of the inulinase produced by the transformant API41 was secreted into the culture. During a 10-L fermentation, 66.2% (w/w) lipid in the yeast cells of the transformant API41 and 14.38 g/L of cell dry weight were attained from inulin of 80.0 g/L within 120 h, high inulinase activity (23.7 U/mL) was also produced within 72 h, and the added inulin was actively hydrolyzed. This confirmed that the genetically engineered yeast of <i>A. melanogenum</i> P10 is suitable for direct production of lipids from inulin. The lipids produced could be used as feedstocks for biodiesel production.
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Dashtban, Mehdi, Greg Kepka, Bernhard Seiboth, and Wensheng Qin. "Xylitol Production by Genetically Engineered Trichoderma reesei Strains Using Barley Straw as Feedstock." Applied Biochemistry and Biotechnology 169, no. 2 (December 18, 2012): 554–69. http://dx.doi.org/10.1007/s12010-012-0008-y.

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Abalde-Cela, Sara, Anna Gould, Xin Liu, Elena Kazamia, Alison G. Smith, and Chris Abell. "High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform." Journal of The Royal Society Interface 12, no. 106 (May 2015): 20150216. http://dx.doi.org/10.1098/rsif.2015.0216.

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Ethanol production by microorganisms is an important renewable energy source. Most processes involve fermentation of sugars from plant feedstock, but there is increasing interest in direct ethanol production by photosynthetic organisms. To facilitate this, a high-throughput screening technique for the detection of ethanol is required. Here, a method for the quantitative detection of ethanol in a microdroplet-based platform is described that can be used for screening cyanobacterial strains to identify those with the highest ethanol productivity levels. The detection of ethanol by enzymatic assay was optimized both in bulk and in microdroplets. In parallel, the encapsulation of engineered ethanol-producing cyanobacteria in microdroplets and their growth dynamics in microdroplet reservoirs were demonstrated. The combination of modular microdroplet operations including droplet generation for cyanobacteria encapsulation, droplet re-injection and pico-injection, and laser-induced fluorescence, were used to create this new platform to screen genetically engineered strains of cyanobacteria with different levels of ethanol production.
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Kang, Qian, Lise Appels, Tianwei Tan, and Raf Dewil. "Bioethanol from Lignocellulosic Biomass: Current Findings Determine Research Priorities." Scientific World Journal 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/298153.

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“Second generation” bioethanol, with lignocellulose material as feedstock, is a promising alternative for first generation bioethanol. This paper provides an overview of the current status and reveals the bottlenecks that hamper its implementation. The current literature specifies a conversion of biomass to bioethanol of 30 to ~50% only. Novel processes increase the conversion yield to about 92% of the theoretical yield. New combined processes reduce both the number of operational steps and the production of inhibitors. Recent advances in genetically engineered microorganisms are promising for higher alcohol tolerance and conversion efficiency. By combining advanced systems and by intensive additional research to eliminate current bottlenecks, second generation bioethanol could surpass the traditional first generation processes.
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Salamanca-Cardona, Lucia, Ryan A. Scheel, Norman Scott Bergey, Arthur J. Stipanovic, Ken'ichiro Matsumoto, Seiichi Taguchi, and Christopher T. Nomura. "Consolidated bioprocessing of poly(lactate-co-3-hydroxybutyrate) from xylan as a sole feedstock by genetically-engineered Escherichia coli." Journal of Bioscience and Bioengineering 122, no. 4 (October 2016): 406–14. http://dx.doi.org/10.1016/j.jbiosc.2016.03.009.

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Culaba, Alvin B., Aristotle T. Ubando, Phoebe Mae L. Ching, Wei-Hsin Chen, and Jo-Shu Chang. "Biofuel from Microalgae: Sustainable Pathways." Sustainability 12, no. 19 (September 28, 2020): 8009. http://dx.doi.org/10.3390/su12198009.

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As the demand for biofuels increases globally, microalgae offer a viable biomass feedstock to produce biofuel. With abundant sources of biomass in rural communities, these materials could be converted to biodiesel. Efforts are being done in order to pursue commercialization. However, its main usage is for other applications such as pharmaceutical, nutraceutical, and aquaculture, which has a high return of investment. In the last 5 decades of algal research, cultivation to genetically engineered algae have been pursued in order to push algal biofuel commercialization. This will be beneficial to society, especially if coupled with a good government policy of algal biofuels and other by-products. Algal technology is a disruptive but complementary technology that will provide sustainability with regard to the world’s current issues. Commercialization of algal fuel is still a bottleneck and a challenge. Having a large production is technical feasible, but it is not economical as of now. Efforts for the cultivation and production of bio-oil are still ongoing and will continue to develop over time. The life cycle assessment methodology allows for a sustainable evaluation of the production of microalgae biomass to biodiesel.
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Dissertations / Theses on the topic "Genetically engineered feedstocks"

1

Liu, Enshi. "FRACTIONATION AND CHARACTERIZATION OF LIGNIN STREAMS FROM GENETICALLY ENGINEERED SWITCHGRASS." UKnowledge, 2017. http://uknowledge.uky.edu/bae_etds/49.

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Development of biomass feedstocks with desirable traits for cost-effective conversion is one of the main focus areas in biofuels research. As suggested by techno-economic analyses, the success of a lignocellulose-based biorefinery largely relies on the utilization of lignin to generate value-added products, i.e. fuels and chemicals. The fate of lignin and its structural/compositional changes during pretreatment have received increasing attention; however, the effect of genetic modification on the fractionation, depolymerization and catalytic upgrading of lignin from genetically engineered plants is not well understood. This study aims to fractionate and characterize the lignin streams from a wild-type and two genetically engineered switchgrass (Panicum virgatum) species (low lignin content with high S/G ratio and high lignin content) using three different pretreatment methods, i.e. dilute sulfuric acid, ammonia hydroxide, and aqueous ionic liquid (cholinium lysinate). The structural and compositional features and impact of lignin modification on lignin-carbohydrate complex characteristics and the deconstruction of cell-wall compounds were investigated. Moreover, a potential way to upgrade low molecular weight lignin to lipids by Rhodococcus opacus was evaluated. Results from this study provide a better understanding of how lignin engineering of switchgrass influences lignin fractionation and upgrading during conversion processes based on different pretreatment technologies.
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