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Journal articles on the topic 'Genotypic Analysis of m morganii'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Zubair, Mohammad, and Ibrahim Mohammad. "Interrelationship of Extended Spectrum Beta-Lactamase Producers and Biofilm Formation among the Gram-Negative Bacteria from Tabuk, KSA." Open Access Macedonian Journal of Medical Sciences 11, A (2023): 15–22. http://dx.doi.org/10.3889/oamjms.2023.11101.

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AIM: The present study investigates the production of extended-spectrum beta-lactamases (ESBL) and the formation of biofilm among different bacterial pathogens. METHODS: The study conducted prospective analysis on bacteria isolates (Gram-negative) from patients who have diagnosed with infections with bacteria between October 2020 and January 2022. RESULTS: The results showed that there were 53 biofilm producers in Escherichia coli. In contrast, Pseudomonas aeruginosa was observed to have the highest percentage, with 32/40 (80%) isolates being biofilm producers. The least number of isolates wer

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Dropa, Milena, Livia C. Balsalobre, Nilton Lincopan, et al. "Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil." Revista do Instituto de Medicina Tropical de São Paulo 51, no. 4 (2009): 203–9. http://dx.doi.org/10.1590/s0036-46652009000400005.

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Extended-spectrum β-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at São Paulo, Brazil, were submitted to analysis by PCR with specific primers for blaSHV, blaTEM and blaCTX-M genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (

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Emborg, Jette, Paw Dalgaard, and Peter Ahrens. "Morganella psychrotolerans sp. nov., a histamine-producing bacterium isolated from various seafoods." International Journal of Systematic and Evolutionary Microbiology 56, no. 10 (2006): 2473–79. http://dx.doi.org/10.1099/ijs.0.64357-0.

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Mesophilic Morganella morganii (n=6) and psychrotolerant M. morganii-like isolates from various seafoods (n=13), as well as clinical M. morganii isolates (n=3), were characterized by using a polyphasic approach including multi-locus sequencing. Based on the phylogenetic analysis, the 22 strains were divided into two distinct groups comprising mesophilic and psychrotolerant isolates, respectively. This classification was supported by DNA–DNA hybridization studies, whereby a psychrotolerant isolate (strain U2/3T) showed 41.0 and 17.8 % relatedness to the type strains of the mesophilic species Mo

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Luo, Xingwei, Yajun Zhai, Dandan He, et al. "Molecular characterization of a novel bla CTX-M-3-carrying Tn6741 transposon in Morganella morganii isolated from swine." Journal of Medical Microbiology 69, no. 8 (2020): 1089–94. http://dx.doi.org/10.1099/jmm.0.001235.

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Introduction. The bla CTX-M-3 gene has rarely been reported in Morganella morganii strains and its genetic environment has not yet been investigated. Aim. To identify the bla CTX-M-3 gene in M. morganii isolated from swine and characterize its genetic environment. Methodology. A M. morganii isolate (named MM1L5) from a deceased swine was identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and subjected to antimicrobial susceptibility testing. The bla genes were detected and then the genetic location and environment of bla CTX-M-3 were in

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Sun, Zhenxiao, Yi Zhang, Xinping Lin, Sufang Zhang, Yingxi Chen, and Chaofan Ji. "Inhibition Mechanism of Lactiplantibacillus plantarum on the Growth and Biogenic Amine Production in Morganella morganii." Foods 12, no. 19 (2023): 3625. http://dx.doi.org/10.3390/foods12193625.

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Morganella morganii, a spoilage bacterium in fermented foods, produces harmful biogenic amines (BAs). Although Lactiplantibacillus plantarum is widely used to inhibit spoilage bacteria, the inhibition pattern and inhibition mechanism of M. morganii by Lpb. plantarum are not well studied. In this study, we analysed the effects of the addition of Lpb. plantarum cell-free supernatant (CFS) on the growth and BA accumulation of M. morganii and revealed the mechanisms of changes in different BAs by using RNA sequencing transcriptome analysis. The results showed that Lpb. plantarum CFS could signific

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Taher, Eman M., Basima A. Abdullah, and Anmar A. AL Taie. "Phenotypic and genotypic detection of biofilm genes for<i> </i><i>Proteus, Morganella, Providencia</i> (PMP) bacterial group isolated from clinical samples of some hospitals in Mosul/Iraq." Journal of Applied and Natural Science 16, no. 4 (2024): 1719–30. https://doi.org/10.31018/jans.v16i4.5935.

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The three genera Proteus spp., Morganella spp., Providencia spp. (PMP) is a group of bacteria belonging to the Enterobacteriaceae family that is predominantly implicated in nosocomial infections within hospital environments. The research aimed to ascertain the frequency of PMP in various clinical samples from some hospitals by isolation and confirming the identification of bacteria from biofilms using Viteck-2 and phenotypic and genotypic determinants of biofilm genes. Out of 380 isolates from different clinical sources that were identified phenotypically (microscopic examination, culture, and

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Barnaud, Guilene, Guillaume Arlet, Charlotte Verdet, Olivier Gaillot, Philippe H. Lagrange та Alain Philippon. "Salmonella enteritidis: AmpC Plasmid-Mediated Inducible β-Lactamase (DHA-1) with anampR Gene from Morganella morganii". Antimicrobial Agents and Chemotherapy 42, № 9 (1998): 2352–58. http://dx.doi.org/10.1128/aac.42.9.2352.

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ABSTRACT DHA-1, a plasmid-mediated cephalosporinase from a single clinicalSalmonella enteritidis isolate, conferred resistance to oxyimino-cephalosporins (cefotaxime and ceftazidime) and cephamycins (cefoxitin and moxalactam), and this resistance was transferable toEscherichia coli HB101. An antagonism was observed between cefoxitin and aztreonam by the diffusion method. Transformation of the transconjugant E. coli strain with plasmid pNH5 carrying the ampD gene (whose product decreases the level of expression of ampC) resulted in an eightfold decrease in the MIC of cefoxitin. A clone with the

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Al Sium, Syed Muktadir, Barna Goswami, Sanjana Fatema Chowdhury, et al. "An insight into the genome-wide analysis of bacterial defense mechanisms in a uropathogenic Morganella morganii isolate from Bangladesh." PLOS ONE 20, no. 1 (2025): e0313141. https://doi.org/10.1371/journal.pone.0313141.

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The gram-negative, facultative anaerobic bacterium Morganella morganii is linked to a number of illnesses, including nosocomial infections and urinary tract infections (UTIs). A clinical isolate from a UTI patient in Bangladesh was subjected to high-throughput whole genome sequencing and extensive bioinformatics analysis in order to gather knowledge about the genomic basis of bacterial defenses and pathogenicity in M. morganii. With an average nucleotide identity (ANI) of more than 97% similarity to a reference genome and phylogenetic analysis verified the isolate as M. morganii. Genome annota

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Behera, Dibyajyoti Uttameswar, Sangita Dixit, Mahendra Gaur, et al. "Sequencing and Characterization of M. morganii Strain UM869: A Comprehensive Comparative Genomic Analysis of Virulence, Antibiotic Resistance, and Functional Pathways." Genes 14, no. 6 (2023): 1279. http://dx.doi.org/10.3390/genes14061279.

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Morganella morganii is a Gram-negative opportunistic Enterobacteriaceae pathogen inherently resistant to colistin. This species causes various clinical and community-acquired infections. This study investigated the virulence factors, resistance mechanisms, functional pathways, and comparative genomic analysis of M. morganii strain UM869 with 79 publicly available genomes. The multidrug resistance strain UM869 harbored 65 genes associated with 30 virulence factors, including efflux pump, hemolysin, urease, adherence, toxin, and endotoxin. Additionally, this strain contained 11 genes related to

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Ramya, D., A. Joseph Thatheyus, S. Jemima Balaselvi Juliana, N. Jennifer Michellin Kiruba, and Deborah Gnana Selvam A. "Physical characterization and kinetic studies of Zn (II) biosorption by Morganella morganii ACZ05." Water Science and Technology 85, no. 4 (2022): 970–86. http://dx.doi.org/10.2166/wst.2022.031.

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Abstract Through this investigation, we establish the mechanism and physical characterization of zinc (II) sequestration by Morganella morganii ACZ05 strain, which was isolated and characterized from soil polluted by effluents from electroplating industries. As far as we know, there is very little literature concerning zinc biosorption using an environmental strain of M. morganii. The SEM analysis shows the dark porous gaps in the aggregated cell-matrix of test bacterial biomass which is inferred as water channels usually seen in biofilms, as compared to metal-unexposed control. M. morganii is

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Ullah, Asad, Sajjad Ahmad, Saba Ismail, et al. "Towards A Novel Multi-Epitopes Chimeric Vaccine for Simulating Strong Immune Responses and Protection against Morganella morganii." International Journal of Environmental Research and Public Health 18, no. 20 (2021): 10961. http://dx.doi.org/10.3390/ijerph182010961.

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Morganella morganii is one of the main etiological agents of hospital-acquired infections and no licensed vaccine is available against the pathogen. Herein, we designed a multi-epitope-based vaccine against M. morganii. Predicted proteins from fully sequenced genomes of the pathogen were subjected to a core sequences analysis, followed by the prioritization of non-redundant, host non-homologous and extracellular, outer membrane and periplasmic membrane virulent proteins as vaccine targets. Five proteins (TonB-dependent siderophore receptor, serralysin family metalloprotease, type 1 fimbrial pr

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Naqqash, Tahir, Aeman Aziz, Muhammad Babar, et al. "Lead-Resistant Morganella morganii Rhizobacteria Reduced Lead Toxicity in Arabidopsis thaliana by Improving Growth, Physiology, and Antioxidant Activities." Agriculture 12, no. 8 (2022): 1155. http://dx.doi.org/10.3390/agriculture12081155.

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Biological remediation serves as a powerful technique for addressing heavy metals toxicity in metals-contaminated soils. The present study aimed to evaluate the efficacy of lead (Pb)-resistant rhizobacterial strains on growth, photosynthetic traits, and antioxidant activities of the Arabidopsis plant under lead toxicity in pot conditions. Two pre-isolated and pre-characterized Pb-resistant Morganella morganii (ABT3) and Morganella morganii (ABT9) strains were used for inoculating Arabidopsis plants grown under varying Pb concentrations (1.5 mM and 2.5 mM) using PbNO3 as the lead source. The tr

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13

Wang, Yazhe, Di Wang, Shengjun Chen, et al. "Genomic Analysis of Two Histamine-Producing Strains Isolated from Yellowfin Tuna." Foods 14, no. 9 (2025): 1532. https://doi.org/10.3390/foods14091532.

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Psychrotrophic Morganella spp. is a typical histamine producer commonly found in seafood, exhibiting a high histamine-producing capacity. In this study, two strains of Morganella (GWT 902 and GWT 904) isolated from yellowfin tuna were subjected to phenotypic and genotypic characterization. Phenotypic analysis reveals differences in growth temperature, NaCl tolerance, and D-galactose fermentation capacity between the two strains. Notably, the histamine production capacity of GWT 902 is significantly higher than that of GWT 904 at 4 °C. The complete genome sequences of strains GWT 902 and GWT 90

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Li, Heling, Zhigang Chen, Qing Ning, Faliang Zong, and Hong Wang. "Isolation and Identification of Morganella morganii from Rhesus Monkey (Macaca mulatta) in China." Veterinary Sciences 11, no. 5 (2024): 223. http://dx.doi.org/10.3390/vetsci11050223.

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A bacterium was isolated and identified from the secretion of a rhesus monkey with endometritis. The morphological results showed that the strain exhibited round, convex, gray-white colonies with smooth surfaces and diameters ranging from 1 to 2 mm when cultured on Columbia blood agar at 37 °C for 24 h; on salmonella–shigella agar (S.S.) at 37 °C for 24 h, the colonies appeared round, flat, and translucent. Gram staining showed negative results with blunt ends and non-spore-forming characteristics. Molecular biology results showed that the 16S rRNA sequence of the strain revealed over 96.9% si

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Sheng, Wang-Huei, Robert E. Badal та Po-Ren Hsueh. "Distribution of Extended-Spectrum β-Lactamases, AmpC β-Lactamases, and Carbapenemases among Enterobacteriaceae Isolates Causing Intra-Abdominal Infections in the Asia-Pacific Region: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART)". Antimicrobial Agents and Chemotherapy 57, № 7 (2013): 2981–88. http://dx.doi.org/10.1128/aac.00971-12.

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ABSTRACTThe increasing trend of β-lactam resistance amongEnterobacteriaceaeis a worldwide threat.Enterobacteriaceaeisolates causing intra-abdominal infections (IAI) from the Study for Monitoring Antimicrobial Resistance Trends (SMART) collected in 2008 and 2009 from the Asia-Pacific region were investigated. Detection of extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases, and carbapenemases was performed by multiplex PCR. A total of 699Enterobacteriaceaeisolates with positive genotypic results, includedEscherichia coli(n= 443),Klebsiella pneumoniae(n= 187),Enterobacter cloacae(n= 45),Kl

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Krutko, V. S., L. H. Nikolaieva, T. V. Maistat, O. A. Oparin, and Anton Viktorovych Rohozhyn. "INFLUENCE OF GENOTYPIC VARIABILITY OF M. TUBERCULOSIS ON THE COURSE OF TUBERCULOSIS WITH MULTIPLE DRUG RESISTANCE." International Medical Journal, no. 1 (March 5, 2020): 68–71. http://dx.doi.org/10.37436/2308-5274-2020-1-15.

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Tuberculosis is infectious and socially dependent disease, being now one of the most pressing issues in practical health care. As well the usual types of tuberculosis infection, chemoresistant tuberculosis is spreading rapidly in the world. The WHO estimates that about 500,000 people on the planet are infected with M. tuberculosis, which is resistant to standard anti−tuberculosis drugs. The probability of successful treatment decreases with emergence of new genotypes of M. tuberculosis with total resistance. In the modern epidemiology of tuberculosis, it is important to identify genotypes on c

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Choi, Sang-Ho, Jung Eun Lee, Su Jin Park та ін. "Emergence of Antibiotic Resistance during Therapy for Infections Caused by Enterobacteriaceae Producing AmpC β-Lactamase: Implications for Antibiotic Use". Antimicrobial Agents and Chemotherapy 52, № 3 (2007): 995–1000. http://dx.doi.org/10.1128/aac.01083-07.

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ABSTRACT Enterobacter spp., Serratia marcescens, Citrobacter freundii, and Morganella morganii are characterized by chromosomally encoded AmpC β-lactamases and possess the ability to develop resistance upon exposure to broad-spectrum cephalosporins. To determine the incidences of the emergence of resistance during antimicrobial therapy for infections caused by these organisms and the effect of the emergence of resistance on patient outcomes, all patients who were admitted to the Asan Medical Center (Seoul, Republic of Korea) from January 2005 to June 2006 and whose clinical specimens yielded E

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18

Bonilla-Aldana, D. Katterine, Jorge Luis Bonilla-Aldana, Juan R. Ulloque-Badaracco, et al. "Snakebite-Associated Infections: A Systematic Review and Meta-Analysis." American Journal of Tropical Medicine and Hygiene 110, no. 5 (2024): 874–86. http://dx.doi.org/10.4269/ajtmh.23-0278.

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ABSTRACT. Snakebites still constitute a significant public health problem in developing countries and are considered a neglected tropical condition by the WHO. Snake accidents are associated with substantial morbidity and mortality and may produce secondary complications, such as severe infections. The objective of this systematic review was to determine the prevalence of snakebite infections and characterize the bacteria isolated from these infections. A systematic literature review in five databases was carried out to assess the prevalence of snakebite infection. A meta-analysis was performe

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Akter, A., MJ Hasan, MA Latif, et al. "Genetic Variability, Heritability, Correlation and Path Coefficient Studies for Yield and Yield Components of Some Promising Rice Hybrids." Bangladesh Rice Journal 23, no. 2 (2020): 27–34. http://dx.doi.org/10.3329/brj.v23i2.48245.

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Eight promising hybrids along with three checks were evaluated for yield and yield contributing traits to observe their genetic variability, heritability, correlation and path coefficient analysis during T. Aman season 2014. The results indicated that the highest genotypic variance was recorded in spikelet panicle-1 followed by effective tiller m-2. Similarly, the highest phenotypic variances were also found with these two characters. Phenotypic coefficient of variation (PCV) was slightly higher than genotypic coefficient of variation (GCV) for all the traits under this study. Hence, slight di

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Hadrich, Ines, Nahed Khemakhem, Amin Ilahi, et al. "Genotypic Analysis of the Population Structure in Malassezia globosa and Malassezia restricta." Journal of Fungi 9, no. 2 (2023): 263. http://dx.doi.org/10.3390/jof9020263.

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The molecular characterization of Malassezia spp. isolates from animals and humans has not been thoroughly studied. Although a range of molecular methods has been developed for diagnosing Malassezia species, they have several drawbacks, such as inefficiency in differentiating all the species, high cost and questionable reproducibility. The present study aimed to develop VNTR markers for genotyping Malassezia isolated from clinical and animal samples. A total of 44 M. globosa and 24 M. restricta isolates were analyzed. Twelve VNTR markers were selected on seven different chromosomes (I, II, III

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Angadi, Airadevi P., B. S. Reddy, R. C. Jagadeesha, Balaji S. Kulkarni, and S. Nishani. "Effect of summer season on correlation coefficient in bird of paradise (Strelitzia reginae) progenies." Journal of Applied and Natural Science 9, no. 1 (2017): 364–69. http://dx.doi.org/10.31018/jans.v9i1.1197.

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The study pertaining to the effect of summer season on correlation analysis in bird of paradise (Strelitzia reginae) was carried out among forty progenies. The results of correlation analysis for twelve parameters (plant height, stem girth, leaf length, leaf width, number of leaves per plant, number of suckers/m 2 , flower stalk length, flower stalk girth, spath length, no. of bracts, vase life and no. of flowers/ m 2 ) at genotypic and phenotypic levels revealed that number of flowers per m 2 of progenies during summer, 2011 showed positive and significant correlations with plant height (0.35

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Petrova, L. V., E. V. Sevastyanova, E. E. Larionova, and T. G. Smirnova. "GENOTYPIC AND PHENOTYPIC COMPARISON OF DRUG RESISTANCE PROFILES OF M. tuberculosis ISOLATES." Вестник ЦНИИТ 7, no. 4 (2023): 23–28. http://dx.doi.org/10.57014/2587-6678-2023-7-4-23-28.

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We conducted a comparative analysis of drug resistance of M. tuberculosis isolates to first-line TB drugs. The diagnostic samples and cultures of M. tuberculosis from TB patients were tested in the Mari El Republic. It was established that the divergence between genotypic and phenotypic isoniazid resistance did not exceed 4%, and rifampicin resistance – 3.9%. In most cases there was lack of mutations associated with resistance to isoniazid or rifampicin in the presence of phenotypic resistance. However, the divergence between results obtained by genotypic and different phenotypic methods was n

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Frothingham, Richard, Percy L. Strickland, Gisela Bretzel, Srinivas Ramaswamy, James M. Musser, and Diana L. Williams. "Phenotypic and Genotypic Characterization ofMycobacterium africanum Isolates from West Africa." Journal of Clinical Microbiology 37, no. 6 (1999): 1921–26. http://dx.doi.org/10.1128/jcm.37.6.1921-1926.1999.

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The Mycobacterium tuberculosis complex includesM. tuberculosis, M. bovis, M. africanum, and M. microti. Most clinical isolates areM. tuberculosis or M. bovis. These species can be distinguished by phenotypes and genotypes. However, there is no simple definition of M. africanum, and some authors question the validity of this species. We analyzed 17 human isolates from Sierra Leone, identified as M. africanum by biochemical and growth characteristics. We sequenced polymorphic genes and intergenic regions. We amplified DNA from six loci with variable numbers of tandem repeats (VNTRs) and determin

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Borelli, Tiago Cabral, Gabriel Lencioni Lovate, Ana Flavia Tonelli Scaranello, et al. "Combining Functional Genomics and Whole-Genome Sequencing to Detect Antibiotic Resistance Genes in Bacterial Strains Co-Occurring Simultaneously in a Brazilian Hospital." Antibiotics 10, no. 4 (2021): 419. http://dx.doi.org/10.3390/antibiotics10040419.

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(1) Background: The rise of multi-antibiotic resistant bacteria represents an emergent threat to human health. Here, we investigate antibiotic resistance mechanisms in bacteria of several species isolated from an intensive care unit in Brazil. (2) Methods: We used whole-genome analysis to identify antibiotic resistance genes (ARGs) and plasmids in 34 strains of Gram-negative and Gram-positive bacteria, providing the first genomic description of Morganella morganii and Ralstonia mannitolilytica clinical isolates from South America. (3) Results: We identified a high abundance of beta-lactamase g

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Hayat, Fawad, Sumera Afzal Khan, Muddasir Khan, et al. "Biological activity of silver nanoparticles synthesized from untapped secondary metabolites of Olea europea endophytic Bacillus amyloliquefaciens." PLOS One 20, no. 5 (2025): e0321134. https://doi.org/10.1371/journal.pone.0321134.

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Endophytic microbes offer frequent contributions to the identification of novel biologically active compounds. The current study aimed to isolate untapped Olea europaea-associated Bacillus amyloliquefaciens and to explore their bioactive secondary metabolites as well as its mediated silver nanoparticles (AgNPs). B. amyloliquefaciens analysis revealed the existence of 23 compounds in metabolite extract by using Gas chromatography-mass spectrometry (GC-MS) analysis. AgNPs were synthesized by using metabolite extract and confirmed by using UV-Vis spectrophotometry, Energy Dispersive Spectroscopy

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Hirabayashi, Aki, Koji Yahara, Satomi Mitsuhashi та ін. "Plasmid analysis of NDM metallo-β-lactamase-producing Enterobacterales isolated in Vietnam". PLOS ONE 16, № 7 (2021): e0231119. http://dx.doi.org/10.1371/journal.pone.0231119.

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Carbapenem-resistant Enterobacterales (CRE) represent a serious threat to public health due to the lack of treatment and high mortality. The rate of antimicrobial resistance of Enterobacterales isolates to major antimicrobials, including carbapenems, is much higher in Vietnam than in Western countries, but the reasons remain unknown due to the lack of genomic epidemiology research. A previous study suggested that carbapenem resistance genes, such as the carbapenemase gene blaNDM, spread via plasmids among Enterobacterales in Vietnam. In this study, we characterized blaNDM-carrying plasmids in

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Recordon-Pinson, Patricia, Cathia Soulié, Philippe Flandre, et al. "Evaluation of the Genotypic Prediction of HIV-1 Coreceptor Use versus a Phenotypic Assay and Correlation with the Virological Response to Maraviroc: the ANRS GenoTropism Study." Antimicrobial Agents and Chemotherapy 54, no. 8 (2010): 3335–40. http://dx.doi.org/10.1128/aac.00148-10.

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ABSTRACT Genotypic algorithms for prediction of HIV-1 coreceptor usage need to be evaluated in a clinical setting. We aimed at studying (i) the correlation of genotypic prediction of coreceptor use in comparison with a phenotypic assay and (ii) the relationship between genotypic prediction of coreceptor use at baseline and the virological response (VR) to a therapy including maraviroc (MVC). Antiretroviral-experienced patients were included in the MVC Expanded Access Program if they had an R5 screening result with Trofile (Monogram Biosciences). V3 loop sequences were determined at screening,

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Cao Yao, Juan Carlos, Jesús Navas Méndez, and María Teresa Tórtola Fernández. "Analysis of Phenotypic and Genotypic Susceptibility to Clarithromycin and Amikacin of Mycobacterium abscessus Complex Strains Isolated from Cystic Fibrosis Patients." Microorganisms 11, no. 12 (2023): 2897. http://dx.doi.org/10.3390/microorganisms11122897.

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Mycobacterium abscessus complex infections are ever on the rise. To curb their increasing evolution, we performed an in-depth study of 43 clinical isolates of cystic fibrosis patients obtained from 2009 to 2020. We identified their subspecies, uncovered their genotypic resistance profiles, characterised their antibiotic-resistant genes, and assessed their phenotypic antibiotic susceptibilities. The phenotypic and genotypic methods showed total agreement in terms of resistance to clarithromycin and amikacin. Of the 43 clinical strains, 28 belonged to M. abscessus subsp. abscessus (65.1%), 13 to

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KIM, SHIN-HEE, BEGOÑA BEN-GIGIREY, JORGE BARROS-VELÁZQUEZ, ROBERT J. PRICE, and HAEJUNG AN. "Histamine and Biogenic Amine Production by Morganella morganii Isolated from Temperature-Abused Albacore." Journal of Food Protection 63, no. 2 (2000): 244–51. http://dx.doi.org/10.4315/0362-028x-63.2.244.

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Histamine-producing bacteria were isolated from albacore stored at 0, 25, 30, and 37°C. They were screened using Niven's differential medium, and their histamine production was confirmed by high-pressure liquid chromatography analysis. The optimum temperature for growth of histamine-producing bacteria was 25°C. The bacterium producing the highest level of histamine was isolated from fish abused at 25°C. It was identified as Morganella morganii by morphological, cultural, biochemical, and antimicrobial characteristics and by the Vitek microbial identification system. The M. morganii isolate was

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Lu, Yanzhi, Jinman Li, and Xuewen Qin. "Study on extended-spectrum beta-lactamases genes and drug resistance in patients with urinary tract infection of enterohemorrhagic Escherichia coli after bladder cancer surgery." Medicine 104, no. 17 (2025): e42098. https://doi.org/10.1097/md.0000000000042098.

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To explore of the detection of enterohemorrhagic Escherichia coli with extended-spectrum beta-lactamase (ESBLs) in patients with urinary tract infections (UTIs) after bladder cancer surgery, and analysis of their genotypic distribution and drug resistance. From February 2022 to February 2024, patients who underwent bladder cancer surgery at our hospital were collected. Among them, those who developed UTIs with enterohemorrhagic E coli postoperatively had their urine specimens isolated and cultured, resulting in 87 strains of enterohemorrhagic E coli. Cultures were conducted on the obtained ent

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KIM, SHIN-HEE, KATHARINE G. FIELD, MICHAEL T. MORRISSEY, ROBERT J. PRICE, CHENG-I. WEI, and HAEJUNG AN. "Source and Identification of Histamine-Producing Bacteria from Fresh and Temperature-Abused Albacore†." Journal of Food Protection 64, no. 7 (2001): 1035–44. http://dx.doi.org/10.4315/0362-028x-64.7.1035.

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Histamine-producing bacteria were isolated from fresh and temperature-abused albacore using two different isolation procedures. Typically, the bacterial isolates on Niven's or modified Niven's medium produced negligible or low levels of histamine (&lt;300 ppm) in histamine enumeration broth. The most frequently found species using this approach was Hafnia alvei. By prescreening on selective media (eosin methylene blue [EMB] agar for enteric bacteria; deMan Rogosa Sharpe agar for lactic acid bacteria; KF streptococcus agar for streptococci; pseudomonas isolation [PI] agar for pseudomonads;

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Terradot, L., J. C. Simon, N. Leterme, et al. "Molecular characterization of clones of the Myzus persicae complex (Hemiptera: Aphididae) differing in their ability to transmit the potato leafroll luteovirus (PLRV)." Bulletin of Entomological Research 89, no. 4 (1999): 355–63. http://dx.doi.org/10.1017/s0007485399000498.

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AbstractA prerequisite to studying the specific interactions involved in the persistent transmission of luteoviruses such as the potato leafroll virus (PLRV) is the characterization of both the virus and its vectors. A range of techniques was used to assess genetic differentiation among 27 clones belonging to the Myzus persicae complex (M. persicae (Sulzer), M. antirrhinii (Macchiati) and M. nicotianaeBlackman) and showing different efficiencies in transmitting PLRV isolates. All M. persicae/M. nicotianae clones belonged to one of two karyotypes, both 2n = 12, either normal or carrying an auto

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Verhaegh, Suzanne J. C., Martine L. Snippe, Foster Levy, et al. "Colonization of healthy children by Moraxella catarrhalis is characterized by genotype heterogeneity, virulence gene diversity and co-colonization with Haemophilus influenzae." Microbiology 157, no. 1 (2011): 169–78. http://dx.doi.org/10.1099/mic.0.042929-0.

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The colonization dynamics of Moraxella catarrhalis were studied in a population comprising 1079 healthy children living in Rotterdam, The Netherlands (the Generation R Focus cohort). A total of 2751 nasal swabs were obtained during four clinic visits timed to take place at 1.5, 6, 14 and 24 months of age, yielding a total of 709 M. catarrhalis and 621 Haemophilus influenzae isolates. Between January 2004 and December 2006, approximate but regular 6-monthly cycles of colonization were observed, with peak colonization incidences occurring in the autumn/winter for M. catarrhalis, and winter/sprin

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Zambounis, Antonios, Eleni Stefanidou, Panagiotis Madesis, Jovana Hrustić, Milica Mihajlović, and Brankica Tanović. "Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis." Plant Protection Science 57, No. 1 (2020): 38–46. http://dx.doi.org/10.17221/35/2020-pps.

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Monilinia laxa, Monilinia fructicola and Monilinia fructigena are the three main causal agents of brown rot, which is one of the most important diseases of stone fruits in pre- and postharvest conditions. Nowadays, the need for the precise genotyping of these Monilinia species in terms of the genetic diversity of their populations or differences in their pathogenicity and host range is a prerequisite for any efficient disease management. In our study, the genetic structure of Monilinia populations in Serbia from three geographically distinct regions was investigated employing <br /> a hi

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Peng, Yousheng, Chenchen Li, Xueke Hui, Xiaoning Huo, Nigus Abebe Shumuyed, and Zhong Jia. "Phenotypic and genotypic analysis of drug resistance in M. tuberculosis isolates in Gansu, China." PLOS ONE 19, no. 9 (2024): e0311042. http://dx.doi.org/10.1371/journal.pone.0311042.

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Tuberculosis has posed a serious threat to human health. It is imperative to investigate the geographic prevalence of tuberculosis and medication resistance, as this information is essential for informing strategies for its prevention and treatment. Drug resistance was identified using a proportion method. Drug-resistant genes and pathways were predicted using whole genome sequencing. The drug resistance range of bedaquiline was identified using the microporous plate two-fold dilution method, and drug resistance genes were studied using sequencing. The study revealed that 19.99% of the tubercu

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Maekawa, Masato, Kayoko Sudo, StevenS L. Li, and Takashi Kanno. "Genotypic analysis of families with lactate dehydrogenase A(M) deficiency by selective DNA amplification." Human Genetics 88, no. 1 (1991): 34–38. http://dx.doi.org/10.1007/bf00204925.

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Huys, G., L. Rigouts, K. Chemlal, F. Portaels, and J. Swings. "Evaluation of Amplified Fragment Length Polymorphism Analysis for Inter- and Intraspecific Differentiation ofMycobacterium bovis, M. tuberculosis, andM. ulcerans." Journal of Clinical Microbiology 38, no. 10 (2000): 3675–80. http://dx.doi.org/10.1128/jcm.38.10.3675-3680.2000.

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The usefulness of amplified fragment length polymorphism (AFLP) analysis was evaluated for the discrimination of Mycobacterium bovis (17 strains), M. tuberculosis (15 strains), andM. ulcerans (12 strains) at the inter- and intraspecific level. The AFLP technique is a whole-genome coverage genotypic fingerprinting method based on the selective PCR amplification of modified restriction fragments obtained through a double enzymatic digest and subsequent ligation of double-stranded restriction site-specific adapter oligonucleotides. Selective amplification ofApaI/TaqI templates with primer combina

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Liu, Y. P., M. A. Behr, P. M. Small, and N. Kurn. "Genotypic Determination of Mycobacterium tuberculosisAntibiotic Resistance Using a Novel Mutation Detection Method, the Branch Migration Inhibition M. tuberculosis Antibiotic Resistance Test." Journal of Clinical Microbiology 38, no. 10 (2000): 3656–62. http://dx.doi.org/10.1128/jcm.38.10.3656-3662.2000.

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A novel method for the detection of any alteration within a defined sequence has recently been demonstrated (A. Lishanski, N. Kurn, and E. F. Ullman, Nucleic Acids Res. 28:E42, 2000; A. Lishanski, Clin. Chem. 46:9, 2000). Essential to this method are the generation of partial duplexes that are capable of forming four-stranded structures and the ability to detect inhibition of branch migration in these structures (I. G. Panyutin and P. Hsieh, J. Mol. Biol. 230:413–424, 1993). Inhibition of branch migration indicates the presence of sequence alteration. This mutation detection method, termed bra

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Khomphet, Thanet, Warin Intana, Athakorn Promwee, and Shams Shaila Islam. "Genetic Variability, Correlation, and Path Analysis of Thai Commercial Melon Varieties." International Journal of Agronomy 2022 (March 10, 2022): 1–6. http://dx.doi.org/10.1155/2022/7877239.

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In the selection phase of melon breeding programs, genetic variability is a critical component for yield improvement. The goals of this study were to discover the variables that affect melon fruit weight and examine genetic variability, correlation, and path analysis of eight melon varieties. The experiment was arranged as a completely randomized block design with 4 blocks. It was conducted between July and September 2021 at the School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, Thailand. The result found that stem diameter and length, leaf length, width, number, and

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Khromova, P. A., S. N. Zhdanova, N. S. Solovieva, et al. "Genotypic and phenotypic characteristics of <i>Mycobacterium tuberculosis</i> drug resistance in TB children." Acta Biomedica Scientifica 7, no. 6 (2022): 82–91. http://dx.doi.org/10.29413/abs.2022-7.6.8.

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Background. Russian Federation is included in the list of 30 countries with the highest burden of tuberculosis, including MDR tuberculosis. The most important part of this problem is the primary MDR/XDR TB in children.The aim: a comparative analysis of the phenotypic and genotypic profile of drug resistance to anti-tuberculosis drugs (ATP) according to whole genome sequencing of M. tuberculosis strains from children.Materials and methods. Whole genome sequencing (WGS) results of 61 M. tuberculosis isolates from children with tuberculosis in 2006–2020 in the Russian Federation were analyzed for

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Yadav, Chandra Bhan, Prakash I. Gangashetty, Manfred Beckmann, Luis A. J. Mur, and Rattan S. Yadav. "Genotype-by-Environment Interaction Analysis of Metabolites in Pearl Millet Genotypes with High Concentrations of Slowly Digestible and Resistant Starch in Their Grains." Cells 11, no. 19 (2022): 3109. http://dx.doi.org/10.3390/cells11193109.

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Genotype × environment interactions (GEIs) should play an important role in the selection of suitable germplasm in breeding programmes. We here assessed GEI effects on pearl millet (Pennisetum glaucum L.) genotypes, selected to possess a high concentration of slowly digestible starch (SDS) and resistant starch (RS) in their grains. Entries were grown in a randomized complete block design with three replications at locations in Bawku-Ghana, Sadore-Niger, Bamako-Mali, Konni-Nigeria, and Gampella-Burkina Faso across West Africa. Harvested grains from these locations were metabolomically profiled

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VOUGIDOU (Χ. ΒΟΥΓΙΔΟΥ), C., V. SANDALAKIS (Β. ΣΑΝΤΑΛΑΚΗΣ), A. PSAROULAKI (Α. ΨΑΡΟΥΛΑΚΗ), E. PETRIDOU (Ε.Ι. ΠΕΤΡΙΔΟΥ), W. DONACHIE, and L. EKATERINIADOU (Λ.ΑΙΚΑΤΕΡΙΝΙΑΔΟΥ). "Serotypic diversity and sequence variation of the ompA gene among Mannheimia haemofytica isolates from domestic ruminants." Journal of the Hellenic Veterinary Medical Society 63, no. 1 (2017): 13. http://dx.doi.org/10.12681/jhvms.15389.

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Pneumonia caused by Mannheìmia haemolytica is an important disease of ruminants. Because of its economic significance, several methods have been used to study the pathogenicity and epidemiology of M. haemolytica. The objectives of this study were to provide data about the prevalence of the different serotypes of the bacterium and to investigate the genetic diversity of a significant virulence factor, the ompA gene. Two methods, DNA sequencing analysis and DGGli, were used to study the polymorphisms of the ompA gene. Ninety-four isolates from pneumonic lungs were investigated. Capsular scrotypi

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Voinescu, Adela, Corina Musuroi, Monica Licker, et al. "Comparative Analysis of Bacterial Conjunctivitis in the Adult and Pediatric Inpatient vs. Outpatient Population." Microorganisms 13, no. 3 (2025): 473. https://doi.org/10.3390/microorganisms13030473.

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The etiology and resistance pattern of bacterial conjunctivitis varies depending on the patient’s care setting and age. A retrospective, observational study was conducted in a tertiary care teaching hospital. A total of 126 patients—76 adults and 50 children—diagnosed with conjunctival infection during inpatient or ambulatory care were analyzed. In the samples of adult patients, isolates were represented by Gram-positive cocci (57.7%; Staphylococcus spp., S. pneumoniae) followed by Enterobacterales (17.97%; P. mirabilis, E. coli, Klebsiella spp.), and non-fermenters (7.69%; Pseudomonas spp., A

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Yousfi, Salima, Maria Dolores Serret та José Luis Araus. "Shoot δ15N gives a better indication than ion concentration or Δ13C of genotypic differences in the response of durum wheat to salinity". Functional Plant Biology 36, № 2 (2009): 144. http://dx.doi.org/10.1071/fp08135.

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We compared the performance of different physiological traits that reveal genotypic variations in tolerance to salinity in durum wheat. A set of 114 genotypes was grown in hydroponics for over 3 months. Three conditions: control, moderate (12 dS m−1) and severe (17 dS m−1) salinity, were maintained for nearly 8 weeks before harvest. The genotype biomass in control conditions correlated with the biomass at the two salinity levels. Subsequently, two subsets of 10 genotypes each were selected on the basis of extreme differences in biomass at the two salinity levels while showing relatively simila

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Sinkov, V. V., and O. V. Ogarkov. "Population structure of the B0/W148 Mycobacterium tuberculosis subtype: Phylogenetic analysis and characteristics of genotypic drug resistance." Acta Biomedica Scientifica 9, no. 4 (2024): 248–59. http://dx.doi.org/10.29413/abs.2024-9.4.27.

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Background. The B0/W148 subtype belongs to the L2phylogenetic lineage of Mycobacterium tuberculosis and is most common in the former Soviet Union. Test systems capable of detecting genetic variants of the pathogen are needed for effective epidemiological surveillance. Studying the genetic diversity of B0/W148 strains and finding molecular markers suitable for their genotyping are key steps in the development of such diagnostic tools.The aim of the work. To study the phylogenetic diversity of the B0/W148 subtype circulating in the territory of the Russian Federation and neighboring countries in

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Piatek, Amy S., Amalio Telenti, Megan R. Murray, et al. "Genotypic Analysis of Mycobacterium tuberculosis in Two Distinct Populations Using Molecular Beacons: Implications for Rapid Susceptibility Testing." Antimicrobial Agents and Chemotherapy 44, no. 1 (2000): 103–10. http://dx.doi.org/10.1128/aac.44.1.103-110.2000.

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ABSTRACT Past genotypic studies of Mycobacterium tuberculosismay have incorrectly estimated the importance of specific drug resistance mutations due to a number of sampling biases including an overrepresentation of multidrug-resistant (MDR) isolates. An accurate assessment of resistance mutations is crucial for understanding basic resistance mechanisms and designing genotypic drug resistance assays. We developed a rapid closed-tube PCR assay using fluorogenic reporter molecules called molecular beacons to detect reportedly common M. tuberculosis mutations associated with resistance to isoniazi

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Polizzi, G., A. Vitale, I. Castello, J. Z. Groenewald, and P. W. Crous. "Cylindrocladium Leaf Spot, Blight, and Crown Rot, New Diseases of Mastic Tree Seedlings Caused by Cylindrocladium scoparium." Plant Disease 90, no. 8 (2006): 1110. http://dx.doi.org/10.1094/pd-90-1110b.

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The mastic tree (Pistacia lentiscus L., Anacardiaceae) is an important sclerophyllous evergreen shrub in the Mediterranean area where it is the dominant component of maquis and garrigues, which is vegetation composed of shrubs, or scrub, usually not exceeding 3 m high. In October 2005, new widespread diseases were noticed in a nursery in eastern Sicily (Italy) affecting container-grown, 1-year-old mastic tree seedlings. Symptoms were detected on approximately 40% of the 5,000 plants and consisted of minute, brown spots, stem lesions, blight, and defoliation. Occasionally, symptoms of crown and

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Akhter, S., JM Ferdous, MR Hossain, and G. Rabbani. "Genetic Analysis and Character Association in Jamir (Citrus jambhiri) Accessions of Bangladesh by Using Morphological Traits." Progressive Agriculture 20, no. 1-2 (2013): 17–26. http://dx.doi.org/10.3329/pa.v20i1-2.16843.

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An experiment was carried out to study the morphological variability, characters association, correlation and path coefficient analysis of 14 Jamir accessions during the period from January 2007 to March 2008. Significant variations were observed for all the plant characters studied. The accession CJ09 was characterized with the maximum plant height (6.20m), spreading of branch (north-south 4.90 m and east-west 4.87m), base girth (58cm), plant growth (102.76m3), tree volume (24.64m3) and segment length (9.70). The accession CJ15 showed maximum spine length (25.33mm), CJ05 was characterized wit

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Nonghanphithak, Ditthawat, Orawee Kaewprasert, Pratchakan Chaiyachat, Wipa Reechaipichitkul, Angkana Chaiprasert, and Kiatichai Faksri. "Whole-genome sequence analysis and comparisons between drug-resistance mutations and minimum inhibitory concentrations of Mycobacterium tuberculosis isolates causing M/XDR-TB." PLOS ONE 15, no. 12 (2020): e0244829. http://dx.doi.org/10.1371/journal.pone.0244829.

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Drug resistance (DR) remains a major challenge for tuberculosis (TB) control. Whole-genome sequencing (WGS) provides the highest genetic resolution for genotypic drug-susceptibility tests (DST). We compared DST profiles of 60 Mycobacterium tuberculosis isolates which were drug resistant according to agar proportion tests (one poly DR-TB, 34 multidrug-resistant TB and 25 extensively drug-resistant TB). We additionally performed minimum inhibitory concentration (MIC) tests using Sensititre MYCOTBI plates (MYCOTB) and a WGS-based DST. Agreement between WGS-based DST and MYCOTB was high for all dr

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Voltas, J., I. Romagosa, A. Lafarga, A. P. Armesto, A. Sombrero, and J. L. Araus. "Genotype by environment interaction for grain yield and carbon isotope discrimination of barley in Mediterranean Spain." Australian Journal of Agricultural Research 50, no. 7 (1999): 1263. http://dx.doi.org/10.1071/ar98137.

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Carbon isotope discrimination (Δ) has been found to be either positively or negatively related to grain yield of small grain cereals when grown in contrasting environments. In order to clarify a possible association between grain yield of barley (Hordeum vulgare L.) and Δ of mature kernels, five 6-rowed and five 2-rowed barley cultivars were evaluated in 22 rainfed environments of northern Mediterranean Spain. Analyses of variance suggested that the genotypic Δ values were more consistent across environments than the genotypic yields. Genotype×environment (G×E) interaction for grain yield was

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