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Journal articles on the topic 'Genotypic Pattern of Pseudomonas Aeruginosa'

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Published: 3 June 2025

Last updated: 31 July 2025

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1

Othman, Hemin E., Eric L. Miller, Jaladet Ms Jubrael, and Ian S. Roberts. "Genotypic characterization and novel multilocus sequence types of exoU+ Pseudomonas aeruginosa from different clinical infections and environments." Innovaciencia Facultad de Ciencias Exactas, Físicas y Naturales 6, no. 1 (2018): 1–14. http://dx.doi.org/10.15649/2346075x.458.

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Introduction: The exoU gene, a marker for highly virulent strains of Pseudomonas aeruginosa, is the major contributor to a wide variety of healthcare-associated infections. Methods: In this study, the antibiotic susceptibility profile, prevalence and genotyping of exoU+ P. aeruginosa were demonstrated. A total of 101 isolates of P. aeruginosa were analysed from different clinical and environmental sources. Results: The antibiotic susceptibility profile classified these isolates as extensively drug resistant (35.6%), multidrug resistant (40.5%) and non-multidrug resistant (23.7%). The prevalenc

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Islam, Nazrul, Dilruba Ahmed, Nazmul Ahsan, Chowdhury R. Ahsan, and Mahmuda Yasmin. "Phenotypic-genotypic Features of MDR Pseudomonas Aeruginosa and Acinetobacter Baumannii From Dhaka, Bangladesh." Bioresearch Communications 9, no. 2 (2023): 1276–84. http://dx.doi.org/10.3329/brc.v9i2.67079.

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Pseudomonas aeruginosa and Acinetobacter baumannii are one of the most common causes of MBL mediated morbidity and mortality throughout the world. Day-by-day these isolates are showing increasing resistance trends to different antimicrobial agents. But there are few data in Bangladesh, the study was designed to observe the pattern of antimicrobial resistance, prevalence of MBL, AmpC and finally phylogenetic distributions. A total of 200 isolates were analyzed in this study, comprising of 100 MDR-carbapenem resistant P. aeruginosa and 100 MDR-carbapenem resistant A. baumonnii. Isolates were tes

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Pal, R. B., and Upasana Pathak. "PFGE GENOTYPING OF METALLO BETA LACTAMASE PRODUCING PSEUDOMONAS AERUGINOSA." International Journal of Advanced Research 10, no. 04 (2022): 29–33. http://dx.doi.org/10.21474/ijar01/14512.

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Purpose: Indiscriminate use of antibiotics since a long time has led to the emergence of organisms resistant to many antibiotics. This study was carried out for genotyping of metallo beta lactamase (MBL) producing P.aeruginosausing pulsed field gel electrophoresis (PFGE) and compare the PFGE pattern with the antibiotic susceptibility pattern obtained. Methods: In this study the MBL producing P.aeruginosa isolates were subjected to antibiotic sensitivity test against 9 antibiotics and further subjected to PFGE to determine their genetic pattern. Results: All the isolates were resistant to merop

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Pal, R. B., and Upasana Pathak. "PFGE GENOTYPING OF METALLO BETA LACTAMASE PRODUCING PSEUDOMONAS AERUGINOSA." International Journal of Advanced Research 10, no. 04 (2022): 29–33. http://dx.doi.org/10.21474/ijar01/14512.

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Purpose: Indiscriminate use of antibiotics since a long time has led to the emergence of organisms resistant to many antibiotics. This study was carried out for genotyping of metallo beta lactamase (MBL) producing P.aeruginosausing pulsed field gel electrophoresis (PFGE) and compare the PFGE pattern with the antibiotic susceptibility pattern obtained. Methods: In this study the MBL producing P.aeruginosa isolates were subjected to antibiotic sensitivity test against 9 antibiotics and further subjected to PFGE to determine their genetic pattern. Results: All the isolates were resistant to merop

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Gholampour Matin, Milad, Reza Shapouri, Mohammadreza Nahaei, Mojtaba Mohammadi Roknabadi, and Rasoul Shokri. "Genotypic Investigation of Antibiotic Resistant blaOXA-4 Gene in Clinical Isolates of Pseudomonas aeruginosa." Avicenna Journal of Clinical Microbiology and Infection 10, no. 3 (2023): 95–99. http://dx.doi.org/10.34172/ajcmi.3471.

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Background: Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, is responsible for 10-15% of hospital infections worldwide. The acquisition of resistance genes is one of the important mechanisms that causes the spread of resistance in this bacterium. This study aimed to conduct a phenotypic and genotypic investigation of the blaOXA-4 resistance gene in P. aeruginosa isolated from clinical samples. Methods: In this study, 110 P. aeruginosa strains were isolated from various clinical samples. The disk diffusion method was applied to reveal the resistance pattern in the isolates. Mo

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Tyumentseva, Marina, Yulia Mikhaylova, Anna Prelovskaya, et al. "CRISPR Element Patterns vs. Pathoadaptability of Clinical Pseudomonas aeruginosa Isolates from a Medical Center in Moscow, Russia." Antibiotics 10, no. 11 (2021): 1301. http://dx.doi.org/10.3390/antibiotics10111301.

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Pseudomonas aeruginosa is a member of the ESKAPE opportunistic pathogen group, which includes six species of the most dangerous microbes. This pathogen is characterized by the rapid acquisition of antimicrobial resistance, thus causing major healthcare concerns. This study presents a comprehensive analysis of clinical P. aeruginosa isolates based on whole-genome sequencing data. The isolate collection studied was characterized by a variety of clonal lineages with a domination of high-risk epidemic clones and different CRISPR/Cas element patterns. This is the first report on the coexistence of

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Sallman, Ruqaia Sabbar, Suzan Saadi Hussein, and Munum Radwan Ali. "ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources." Al-Mustansiriyah Journal of Science 29, no. 2 (2018): 50. http://dx.doi.org/10.23851/mjs.v29i2.345.

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Recently, Pseudomonas aeruginosa infections proportions have increased significantly. Molecular typing and virulence analysis are good techniques, which can lead us to know P. aeruginosa infections. P. aeruginosa isolates were identified by using molecular method (16S rDNA gene) via PCR technique for accurate identification. The highest percent 41.26% of P. aeruginosa bacteria was found in the burn infections followed by 28.57% in wound swabs, 17.46% in ear discharge and lowest percentage were obtained from sputum samples. All isolates classified into six groups (A-F) according to classes of a

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Shawky, Mohamed, Waleed B. Suleiman, and Ayman A. Farrag. "Antibacterial Resistance Pattern in Clinical and Non-clinical Bacteria by Phenotypic and Genotypic Assessment." Journal of Pure and Applied Microbiology 15, no. 4 (2021): 2270–79. http://dx.doi.org/10.22207/jpam.15.4.49.

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Bacterial infections represent a very serious problem that threatens human health, antibiotics were designed to attack the causative agents of infectious diseases, but some bacterial pathogens became virulent and resistant to antibiotics by different mechanisms, resistance genes represented one of those mechanisms. This study attempts to screen the existence of five different resistance genes (mecA, TEM, FemA, MexD, and AmpC) among 25 bacterial isolates divided into two groups the first was non-clinical bacterial type strains including Bacillus subtilis, Escherichia coli, Klebsiella pneumonia,

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Elmouaden, Chaimae, Amin Laglaoui, Latifa Ennanei, Mohammed Bakkali, and Mohammed Abid. "Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco." Journal of Infection in Developing Countries 13, no. 10 (2019): 892–98. http://dx.doi.org/10.3855/jidc.10675.

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Introduction: Pseudomonas aeruginosa is an ubiquitous bacterium causes various community-acquired and nosocomial infections. In this investigation, we aimed to screen the antibiotic susceptibility patterns and the prevalence of virulence factor genes in a set of Pseudomonas aeruginosa isolated from nosocomial and community-acquired infections in the Northwestern of Morocco. Methodology: A total of 155 of Pseudomonas aeruginosa strains were collected (January 2015 - December 2016) from nosocomial and community-acquired infections at hospital centers and clinical laboratories in the Northw

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Vasiljevic, Zorica, B. Jovcic, Ivana Cirkovic, and Slobodanka Djukic. "An examination of potential differences in biofilm production among different genotypes of Pseudomonas aeruginosa." Archives of Biological Sciences 66, no. 1 (2014): 117–21. http://dx.doi.org/10.2298/abs1401117v.

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In the present study, we have examined if there is any difference in biofilm production among different genotypes of Pseudomonas aeruginosa. The study investigated 526 non-duplicate P. aeruginosa isolated from clinical specimens and from a hospital environment. Isolates were grouped into thirty-five genotypes based on an identical ERIC2-band pattern. Biofilm formation was quantified by the microtiter plate test and all strains were classified into the following categories: no biofilm producers (0), weak (+), moderate (+), or strong (+++) biofilm producers. Only 2.45% of examined strains were n

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Alamu, Juliana, Leela Kakithakara Vajravelu, Balamurali Venkatesan, and Jayaprakash Thulukanam. "Correlation of phenotypic and genotypic virulence markers, antimicrobial susceptibility pattern, and outcome of Pseudomonas aeruginosa sepsis infection." Microbial Pathogenesis 170 (September 2022): 105716. http://dx.doi.org/10.1016/j.micpath.2022.105716.

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Pourmohammadi, Naser, Reza Shapouri, Mohammadreza Nahaei, and Mojtaba Mohammadi Roknabadi. "Genotypic Investigation of Antibiotic Resistance: Presence of BlaOXA-48 Gene in Clinical Isolates of Pseudomonas aeruginosa." Avicenna Journal of Clinical Microbiology and Infection 11, no. 2 (2024): 62–67. http://dx.doi.org/10.34172/ajcmi.3508.

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Background: Identifying antimicrobial resistance patterns and antibiotic resistance genes in clinical isolates of Pseudomonas aeruginosa is an important strategy for effectively combating infections. This study aimed to identify the antimicrobial resistance patterns and the presence of blaOXA-48 beta-lactamase gene in P. aeruginosa isolated from clinical samples. Methods: We collected 100 isolates of P. aeruginosa from different clinical samples, including urine, tracheal tube, blood, and burn wounds. The antimicrobial resistance patterns of the isolates were investigated using the disk diffus

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Abaza, Amani F., Soraya A. El Shazly, Heba S. A. Selim, and Gehan S. A. Aly. "Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa in a Healthcare Setting in Alexandria, Egypt." Polish Journal of Microbiology 66, no. 3 (2017): 297–308. http://dx.doi.org/10.5604/01.3001.0010.4855.

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Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed countries. In this work we aimed at studying the occurrence of metallo-beta-lactamase (MBL) producing P. aeruginosa in a healthcare setting in Alexandria, Egypt. This cross sectional study included 1583 clinical samples that were collected from patients admitted to Alexandria University Students’ Hospital. P. aeruginosa isolates were identified using standard microbiological methods and were tested for their antimicrobial susceptibility pat

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Tadjrobehkar, Omid, and Atefeh Kamali. "Evaluation of Antibiotic Resistance Pattern and Extended-Spectrum Beta-lactamases in Pseudomonas aeruginosa Isolates Obtained from Clinical Samples by Phenotypic and Genotypic Methods in Zabol, Iran." Journal of Kerman University of Medical Sciences 29, no. 6 (2022): 529–35. http://dx.doi.org/10.34172/jkmu.2022.64.

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Background: Pseudomonas aeruginosa is a human opportunistic pathogen that is known to be responsible for various diseases. However, its antibiotic-resistant isolates often cause serious infections. Methods: This study for the first time investigated a total of 80 P. aeruginosa isolates collected from patients admitted to Amir Al-Momenin hospital. The isolates were identified by biochemical assays. The combination disc test method was used to measure antibiotic susceptibility and confirm the presence of extended spectrum-beta lactamases-producing enzymes. Also, the presence of enzyme-producing

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M. Ismail, Yasser, Sahar M. Fayed, Fatma M. Elesawy, Nora Z Abd El-Halim, and Ola S. El-Shimi. "Phenotypic and Molecular Characteristics of Pseudomonas Aeruginosa Isolated from Burn Unit." EJMM-Volume 30-Issue 1 30, no. 1 (2021): 19–28. http://dx.doi.org/10.51429/ejmm30103.

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Background: The biggest concern for a burn team is a nosocomial infection in burn patients, which is a significant health issue. Pseudomonas aeruginosa is an extremely troublesome drug-resistant bacterium in the world today. We are now faced with rising P. aeruginosa pan-drug-resistant clones in hospital settings. Objectives: To evaluate the distribution of different virulence factors generated by P. aeruginosa isolated from burn wound infections, together with its antimicrobial susceptibility. Methodology: The isolates reported as P. aeruginosa were further tested for the presence of various

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Saikumar, Chitralekha, Punithavathi Velmurugan, and Aishwarya Jothi Ramalingam. "Bacteriological Profile, Drug Resistance Pattern and its Molecular Characterisation Among Patients with Chronic Suppurative Otitis Media at a Tertiary Care Hospital in South India." Biomedical and Pharmacology Journal 17, no. 4 (2024): 2319——2327. https://doi.org/10.13005/bpj/3027.

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Millions of people worldwide suffer from chronic suppurative otitis media (CSOM), a recurrent infection of the ear that is particularly common in low-resource environments. Although there are several etiological factors, bacterial infection is a significant one. The two most frequently isolated bacteria are Staphylococcus aureus and Pseudomonas aeruginosa. Due to the emergence and spread of bacterial resistance to several antimicrobial agents and also their ability to form biofilms, treatment and recovery are made more challenging. Our aim was to identify the clinico-bacteriological profile, t

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Orokor, Ordu, Wisdom, Mbata, C.A., and Monsi Tombari Pius. "Molecular Profiling of Antibiotic-resistant Pseudomonas aeruginosa Isolated from Patients with Urinary Tract Infections in Rivers State University Teaching Hospital." International Journal of Pathogen Research 14, no. 1 (2025): 27–41. https://doi.org/10.9734/ijpr/2025/v14i1338.

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Background: Antibiotic-resistant Pseudomonas aeruginosa poses a significant challenge in urinary tract infections (UTIs), particularly in hospital settings. Aim: The aim of this study was to analyze the molecular profile of antibiotic-resistant P. aeruginosa strains isolated from patients with urinary tract infections at the Rivers State University Teaching Hospital, Port Harcourt. Methods: As part of a cross-sectional study, 199 patients were interviewed, and urine samples were collected from subjects with symptoms of a urinary tract infection. A semiquantitative urine culture method was used

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Qing, Yun, Kai-Yuan Cao, Zhi-Li Fang та ін. "Outbreak of PER-1 and diversity of β-lactamases among ceftazidime-resistant Pseudomonas aeruginosa clinical isolates". Journal of Medical Microbiology 63, № 3 (2014): 386–92. http://dx.doi.org/10.1099/jmm.0.069427-0.

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A growing number of β-lactamases have been reported in Pseudomonas aeruginosa clinical isolates. The aim of this study was to investigate the diversity of β-lactamases in the collection of 51 ceftazidime-resistant P. aeruginosa clinical isolates in four hospitals of southern China. Among these isolates, variable degrees of resistance to other β-lactam and non-β-lactam agents were observed. Pulsed-field gel electrophoresis (PFGE) revealed a high degree of clonality with five main genotypes. Of the 51 isolates tested, 35 (68.6 %) were identified as extended-spectrum β-lactamase (ESBL) producers,

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Milojković, Marko, Željka Nenadović, Slaviša Stanković, et al. "Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia." Archives of Industrial Hygiene and Toxicology 71, no. 3 (2020): 231–50. http://dx.doi.org/10.2478/aiht-2020-71-3418.

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AbstractDrug resistance of Pseudomonas aeruginosa is a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonas spp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of iso

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Bragonzi, Alessandra, Lutz Wiehlmann, Jens Klockgether, et al. "Sequence diversity of the mucABD locus in Pseudomonas aeruginosa isolates from patients with cystic fibrosis." Microbiology 152, no. 11 (2006): 3261–69. http://dx.doi.org/10.1099/mic.0.29175-0.

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The mucA gene of the muc operon, which is instrumental in the control of the biosynthesis of the exopolysaccharide alginate, is a hotspot of mutation in Pseudomonas aeruginosa, a micro-organism that chronically colonizes the airways of individuals with cystic fibrosis (CF). The mucA, mucB and mucD genes were sequenced in nine environmental isolates from aquatic habitats, and in 37 P. aeruginosa strains isolated from 10 patients with CF, at onset or at a late stage of chronic airway colonization, in order to elucidate whether there was any association between mutation and background genotype. T

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Tassios, Panayotis T., Vassiliki Gennimata, Anthony N. Maniatis, Caroline Fock, Nicholas J. Legakis, and The Greek Pseudomonas aeruginosa Study Group. "Emergence of Multidrug Resistance in Ubiquitous and Dominant Pseudomonas aeruginosa Serogroup O:11." Journal of Clinical Microbiology 36, no. 4 (1998): 897–901. http://dx.doi.org/10.1128/jcm.36.4.897-901.1998.

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The serotypes of 88 nonreplicate nosocomial Pseudomonas aeruginosa isolates from 11 Greek hospitals were studied in relation to their antibiotic susceptibilities. Rates of resistance to β-lactams, aminoglycosides, and quinolones ranged from 31 to 65%, except for those to ceftazidime (15%) and imipenem (21%). Four serotypes were dominant: O:12 (25% of isolates), O:1 (17%), O:11 (16%), and O:6 (10%). Multidrug resistance rates in the major serogroups O:12 (91%) and O:11 (79%) were higher than those in serogroups O:1 (40%) and O:6 (43%). Further typing with respect to pulsed-field gel electrophor

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Silverio, Myllena Pereira, Gabriela Bergiante Kraychete, Alexandre Soares Rosado, and Raquel Regina Bonelli. "Pseudomonas fluorescens Complex and Its Intrinsic, Adaptive, and Acquired Antimicrobial Resistance Mechanisms in Pristine and Human-Impacted Sites." Antibiotics 11, no. 8 (2022): 985. http://dx.doi.org/10.3390/antibiotics11080985.

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Pseudomonas spp. are ubiquitous microorganisms that exhibit intrinsic and acquired resistance to many antimicrobial agents. Pseudomonas aeruginosa is the most studied species of this genus due to its clinical importance. In contrast, the Pseudomonas fluorescens complex consists of environmental and, in some cases, pathogenic opportunistic microorganisms. The records of antimicrobial-resistant P. fluorescens are quite scattered, which hinders the recognition of patterns. This review compiles published data on antimicrobial resistance in species belonging to the P. fluorescens complex, which wer

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Peña, C., A. Guzmán, C. Suarez, et al. "Effects of Carbapenem Exposure on the Risk for Digestive Tract Carriage of Intensive Care Unit-Endemic Carbapenem-Resistant Pseudomonas aeruginosa Strains in Critically Ill Patients." Antimicrobial Agents and Chemotherapy 51, no. 6 (2007): 1967–71. http://dx.doi.org/10.1128/aac.01483-06.

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ABSTRACT To determine the epidemiology and risk factors for carbapenem-resistant Pseudomonas aeruginosa (CR-PA) digestive tract colonization, weekly rectal and pharyngeal swabs were obtained in two serial incidence surveys (266 patients). Forty-two (16%) patients were CR-PA colonized (12 [29%] on admission and 30 [71%] in intensive care units). Pulsed-field gel electrophoresis showed extensive clonal diversity, although one specific clone (type B) was isolated from 11 patients. The presence of similar genotypes of CR-PA colonizing 30% of the CR-PA-colonized patients suggests the occurrence of

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Abid, Muhammad Bilal, Blake Buchan, Nathan Ledeboer, L. Silvia Munoz-Price та Mary Beth Graham. "1192. Identification of a Novel Enterobacter cloacae Isolate Producing an IMP-13 Metallo-β-Lactamase". Open Forum Infectious Diseases 5, suppl_1 (2018): S360—S361. http://dx.doi.org/10.1093/ofid/ofy210.1025.

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Abstract Background Metallo-β-lactamases (MBLs) have been identified as emerging resistance determinants in Enterobacteriaceae, A. baumanii, and P. aeruginosa. Early identification of carbapenemase-producing organisms (CPOs) is essential to prevent dissemination within healthcare settings. We report a case of a patient who was blood culture positive for a multidrug-resistant E. cloacae which was subsequently found to be positive for the MBL blaIMP-13. Methods A 74-year-old female, with no significant past medical or travel history, developed sepsis 2 days after undergoing debulking surgery for

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Shiny, PA, S. Rajendran, and YLakshmi Sarayu. "A comparative analysis of isolation and antibiotic sensitivity pattern of Pseudomonas aeruginosa isolated from pus and urine with special reference to phenotypic and genotypic expression of extended spectrum beta lactamases (ESBLs)." Journal of The Academy of Clinical Microbiologists 15, no. 1 (2013): 3. http://dx.doi.org/10.4103/0972-1282.116083.

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Garza-González, Elvira, Paola Bocanegra-Ibarias, Miriam Bobadilla-del-Valle, et al. "Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species: Results from the infivar network." PLOS ONE 16, no. 3 (2021): e0248614. http://dx.doi.org/10.1371/journal.pone.0248614.

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Aim This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the W

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Maeda, Yoshiaki, Yui Sugiyama, Atsushi Kogiso, et al. "Colony Fingerprint-Based Discrimination of Staphylococcus species with Machine Learning Approaches." Sensors 18, no. 9 (2018): 2789. http://dx.doi.org/10.3390/s18092789.

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Detection and discrimination of bacteria are crucial in a wide range of industries, including clinical testing, and food and beverage production. Staphylococcus species cause various diseases, and are frequently detected in clinical specimens and food products. In particular, S. aureus is well known to be the most pathogenic species. Conventional phenotypic and genotypic methods for discrimination of Staphylococcus spp. are time-consuming and labor-intensive. To address this issue, in the present study, we applied a novel discrimination methodology called colony fingerprinting. Colony fingerpr

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ANDERSSON, C., A. DRAGOMIR, L. HJELTE, and G. M. ROOMANS. "Cystic fibrosis transmembrane conductance regulator (CFTR) activity in nasal epithelial cells from cystic fibrosis patients with severe genotypes." Clinical Science 103, no. 4 (2002): 417–24. http://dx.doi.org/10.1042/cs1030417.

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Cystic fibrosis is a heterogenic disease, in which the phenotype can also vary for patients with the same genotype. In the present study the function of the cystic fibrosis transmembrane conductance regulator (CFTR) in nasal epithelial cells from 19 adult patients with cystic fibrosis was investigated. All patients had severe mutations, whereby no or little functional CFTR is expected in the plasma membrane. Of the patients, 15 were homozygous for ΔF508-CFTR (i.e. CTFR lacking residue Phe-508). The others were ΔF508-heterozygous with 3659delC, 394delTT or 2183AA→G. Nasal epithelial cells, obta

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Shekar, Koustubh, Akanksha Chichra, Keshav Garg, et al. "Molecular Patterns of Resistance in Carbapenem Resistant Organisms Detected in Stool Surveillance Cultures in Patients Undergoing Hematopoietic Cell Transplant." Blood 142, Supplement 1 (2023): 4918. http://dx.doi.org/10.1182/blood-2023-184659.

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Introduction Patients undergoing hematopoietic stem cell transplant (HSCT) are at high risk of life-threatening bacterial infections. Although the prevalence and pattern of resistance varies amongst centres and countries, there is a growing global problem of resistance to antibiotics. The increased use of broad-spectrum antibiotics and injudicious antibiotic policies have contributed to the selection of multidrug resistant(MDR) pathogens. Translocation of organisms which normally colonize the gut into the bloodstream during the febrile neutropenia has long been postulated as a pathogenic facto

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Hamasaeed, Payman Akram. "Resistance to bacterial infection, complication occurring after cardiac surgery." Cellular and Molecular Biology 66, no. 8 (2020): 20–25. http://dx.doi.org/10.14715/cmb/2020.66.8.4.

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To analyze the occurrence of resistant bacterial infection in patients undergoing cardiac surgery hospitalized in the surgical specialty hospital, in Erbil city, Iraq. A prospective study was done on a total of 138 patients operated and hospitalized in an intensive care unit and surgical wards. Bacterial isolates identification was done according to cultural characteristics, microscopic examination, some biochemical tests, analytic Profile Index 20E& API Staph, confirmed with VITEK® 2 compact system (BioMérieux). Antimicrobial susceptibility for disc diffusion tested to 17 antimicrobial ag

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Eswani, Mohamed, Ashraf Naass, Nagib A. Elmarzugi, Ahmed M. Elmarghani, Mohammed Elfgee, and Mohammed O. Elbasir. "APPLICATION OF PHENOTYPIC AND GENOTYPIC STRATEGIES TO IDENTIFY PSEUDOMONAS AERUGINOSA." EPH - International Journal of Applied Science 7, no. 3 (2021): 11–15. http://dx.doi.org/10.53555/eijas.v7i3.52.

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A case study of patient presented with developed wound infection with Pseudomonas aeruginosa (leukocytosis). The strains of P. aeruginosa isolated from Sheffield General Northern Hospital (UK) and studied for phenotypic and genotypic identifications. This present study were conducted in Sheffield Hallam University (UK) and was aimed to evaluate the application of Phenotypic and Genotypic techniques to identify P. aeruginosa. Generally, the results of Real time PCR (RTPCR) revealed that the diagnosis viability was confirmed for P. aeruginosa. However, identification on the basis of phenotype by

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Kadry, Ashraf, Marwa Ezz, and Hisham A. Abbas. "Phenotypic and genotypic characterization of Pseudomonas aeruginosa siderophores." Zagazig Journal of Pharmaceutical Sciences 27, no. 1 (2018): 22–30. http://dx.doi.org/10.21608/zjps.2018.38110.

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Upadhyay, Deepa, and Anshu Sharma. "Detection of Metallo-Beta-Lactamase Encoding Genes by Multiplex RT-PCR among Clinical Isolates of Pseudomonas Aeruginosa." International Journal of Health Sciences and Research 14, no. 9 (2024): 256–59. http://dx.doi.org/10.52403/ijhsr.20240933.

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Introduction: Metallo-beta-lactamase producing Pseudomonas aeruginosa is an important emerging nosocomial pathogen, due to its ability to acquired drug resistance. Aim & objectives: The aim of this study was to detect MBL encoding genes by genotypic method among clinical isolates of Pseudomonas aeruginosa. Material & Method: A total of 50 non-repetitive isolates of P. aeruginosa recovered from various clinical samples were included in this study. All these isolates were screened for MBL production by RT-PCR irrespective of imipenem sensitivity and the presence of blaVIM, blaIMP & b

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Pokharel, Khilasa, Bishwa Raj Dawadi, Chandra Prakash Bhatt, and Satish Gupte. "Prevalence of Pseudomonas aeruginosa and its Antibiotic Sensitivity Pattern." Journal of Nepal Health Research Council 17, no. 01 (2019): 109–13. http://dx.doi.org/10.33314/jnhrc.v17i01.1877.

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Background: Pseudomonas aeruginosa is an opportunistic pathogen which causes most of the chronic infection in humans. This study was undertaken to determine the prevalence rate of Pseudomonas aeruginosa that is isolated from various clinical specimens along with its antibiotic susceptibility pattern.Methods: This descriptive cross sectional study was conducted in Kathmandu Medical College and Teaching Hospital (KMCTH) from February to May 2018. Pseudomonas aeruginosa isolated from various clinical specimens were processed in clinical laboratory, Department of Microbiology, KMCTH. Isolation, id

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Yamaguchi, Satoshi, Takashi Suzuki, Takeshi Kobayashi, et al. "Genotypic analysis of Pseudomonas aeruginosa isolated from ocular infection." Journal of Infection and Chemotherapy 20, no. 7 (2014): 407–11. http://dx.doi.org/10.1016/j.jiac.2014.02.007.

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Parsek, Matthew R., and Tim Tolker-Nielsen. "Pattern formation in Pseudomonas aeruginosa biofilms." Current Opinion in Microbiology 11, no. 6 (2008): 560–66. http://dx.doi.org/10.1016/j.mib.2008.09.015.

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ABOU-DOBARA, M. I., M. A. DEYAB, E. M. ELSAWY, and H. H. MOHAMED. "Antibiotic Susceptibility and Genotype Patterns of Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa Isolated from Urinary Tract Infected Patients." Polish Journal of Microbiology 59, no. 3 (2010): 207–12. http://dx.doi.org/10.33073/pjm-2010-032.

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Thirty nine isolates of Escherichia coli, twenty two isolates of Klebsiella pneumoniae and sixteen isolates of Pseudomonas aeruginosa isolated from urinary tract infected patients were analyzed by antimicrobial susceptibility typing and random amplified polymorphic DNA (RAPD)-PCR. Antibiotic susceptibility testing was carried out by microdilution and E Test methods. From the antibiotic susceptibility, ten patterns were recorded (four for E. coli, three for K. pneumoniae and three for P. aeruginosa respectively). Furthermore, genotyping showed seventeen RAPD patterns (seven for E. coli, five fo

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Egbule, O. S. "Antibiotic Resistance and Transfer of Extended Spectrum Beta Lactamase (ESBL) Resistant Plasmids in Pseudomonas aeruginosa Isolates from Hospitalized Patients." Nigerian Journal of Basic and Applied Sciences 29, no. 1 (2022): 90–99. http://dx.doi.org/10.4314/njbas.v29i1.11.

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The study was undertaken to determine antibiotic resistance, ESBL production and transfer of resistant plasmids of Pseudomonas aeruginosa. Clinical samples of urine, wound and blood were screened for Pseudomonas aeruginosa following standard microbiological procedures. Isolates of Pseudomonas aeruginosa were identified in a total of 110 (18.3%) samples with higher prevalence observed in wound (43.6%) and blood (40.0%) samples. Identified isolates were profiled for antimicrobial susceptibility to a total of 10 antibiotics. Plasmid DNA extraction was carried out by the alkaline lysis method. Dou

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González-Olvera, Eliab M., Rebeca Pérez-Morales, Alberto González Zamora, Graciela Castro-Escarpulli, Ingrid Palma-Martínez, and José J. Alba-Romero. "Antibiotic resistance, virulence factors and genotyping of Pseudomonas aeruginosa in public hospitals of northeastern Mexico." Journal of Infection in Developing Countries 13, no. 05 (2019): 374–83. http://dx.doi.org/10.3855/jidc.10953.

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Introduction: Pseudomonas aeruginosa is the second most prevalent opportunistic pathogen causing nosocomial infections in Mexico. This study evaluated antibiotic resistance, production of virulence factors and clonal diversity of P. aeruginosa strains isolated from patients undergoing nosocomial infections in public hospitals of northeastern Mexico. Methodology: Ninety-two P. aeruginosa isolates from urine culture, Foley catheter, ear, wounds, respiratory tract secretions, scalp, blood culture, bronchoalveolar lavage, expectoration and cerebrospinal fluid causing nosocomial infections we

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Elkhatib, Walid F., Mahmoud A. F. Khalil, and Hossam M. Ashour. "Integrons and Antiseptic Resistance Genes Mediate Resistance of Acinetobacter baumannii and Pseudomonas aeruginosa Isolates from Intensive Care Unit Patients with Wound Infections." Current Molecular Medicine 19, no. 4 (2019): 286–93. http://dx.doi.org/10.2174/1566524019666190321113008.

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Background: Acinetobacter baumannii and Pseudomonas aeruginosa are of major concern for hospitalized patients. Methods: We evaluated antibiotic and antiseptic resistance of A. baumannii (n = 29) and P. aeruginosa (n = 37) isolates recovered from 66 intensive care unit (ICU) patients and determined the prevalence of qacE, qacEΔ1, and integrons in these clinical isolates. Antibiotic and antiseptic susceptibility testing was performed via Kirby Bauer disk diffusion and broth microdilution methods, respectively. The resistance genes and integrons were detected by PCR. A. baumannii and P. aeruginos

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B, Apoorva, Sneha Mohan, Mukesh Manjhi, and Dalip Kakru. "Comparison of Antibiotic Susceptibility Pattern of Pseudomonas aeruginosa from Critical and Non-Critical Areas at a Tertiary Care Hospital." Journal of Evidence Based Medicine and Healthcare 8, no. 12 (2021): 677–81. http://dx.doi.org/10.18410/jebmh/2021/133.

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BACKGROUND Pseudomonas aeruginosa is the most common gram-negative bacterium associated with nosocomial infections. Active observation of changes in antibiotic resistance of Pseudomonas aeruginosa is necessary for the selection of appropriate antimicrobial agent for empirical therapy. This study was conducted to determine the antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from various clinical samples collected from patients admitted in critical and non-critical areas. METHODS Pseudomonas aeruginosa isolates obtained from various samples in critical and non-critical areas

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Chaudhary, Jitendra Kumar. "A Clinical-Bacteriological Profile of Multidrug Resistant Pseudomonas aeruginosa at Tertiary Care Hospital Shahjahanpur, Uttar Pradesh." International Journal of Current Microbiology and Applied Sciences 10, no. 9 (2021): 301–13. http://dx.doi.org/10.20546/ijcmas.2021.1009.035.

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The Pseudomonas aeruginosa is predominant agent causing nosocomial infections. In recent time, it develops resistance continuously to the antibiotics becomes Multidrug-resistant P. aeruginosa. So, in cystic fibrosis patents it difficult to eradicate P. aeruginosa infections with antimicrobial treatment. Therefore, focus on alternative mechanisms for treating P. aeruginosa infections. On the basis of growth, morphological and biochemical characteristics, P. aeruginosa strains were isolated from the clinical samples in this work. After that the antibiotic sensitivity was performed and the Multid

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Khalili, Younes, Mohammad Yousef Memar, Safar Farajnia, Khosro Adibkia, Hossein Samadi Kafil, and Reza Ghotaslou. "Molecular epidemiology and carbapenem resistance of Pseudomonas aeruginosa isolated from patients with burns." Journal of Wound Care 30, no. 2 (2021): 135–41. http://dx.doi.org/10.12968/jowc.2021.30.2.135.

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Objective: The aim of this study was to investigate the molecular epidemiology and carbapenem resistance mechanisms of Pseudomonas aeruginosa isolated from patients with burns in Azerbaijan, Iran. Method: Pseudomonas aeruginosa was isolated from 38 patients with burns. Disk diffusion and agar dilution methods were used to determine antibiotic susceptibility patterns. The overproduction of AmpC β-lactamase and efflux pumps were detected by phenotypic methods. The presence of carbapenemase-encoding genes was detected by multiplex polymerase chain reaction (PCR). Expression of the OprD gene and M

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Bakhat, Shaista, Yasmeen Taj, Faisal Hanif, and Saman Nadeem. "BlaOXA-48 Genotypic Detection of Carbapenem Resistance in Isolates of Pseudomonas Aeruginosa." Journal of Bahria University Medical and Dental College 10, no. 02 (2021): 89–93. http://dx.doi.org/10.51985/jbumdc2019133.

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Objective: To determine the prevalence of carbapenem resistance in strains of Pseudomonas aeruginosa at a molecular level by detecting OXA-48 gene which transcribe for resistance to the antibiotic carbapenem among indoor patients of a tertiary care hospital Karachi. Study Design and Setting: This observational cross-sectional study was conducted from September 2018 to May 2019 at PNS Shifa hospital of Karachi. Methodology: Total 140 strains of Pseudomonas aeruginosa were received and cultured from pus samples. These samples were collected from different wards like medicine, surgery, burn unit,

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa." Journal of Tropical Diseases 4, no. 1 (2015). https://doi.org/10.5281/zenodo.166324.

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<strong>Introduction:</strong> Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. <strong>Methods:</strong> P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemic

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa." Journal of Tropical Diseases 4, no. 1 (2015). https://doi.org/10.5281/zenodo.193946.

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Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number u

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, and Gopal Nayak. "Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa." Omics Publishing Group, October 7, 2015. https://doi.org/10.5281/zenodo.813958.

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Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi's biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using a

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Kumar Trivedi, Mahendra. "Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa." Journal of Tropical Diseases 04, no. 01 (2016). http://dx.doi.org/10.4172/2329-891x.1000181.

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Kamali, Esmat, Ailar Jamali, Abdollah Ardebili, Freshteh Ezadi, and Alireza Mohebbi. "Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa." BMC Research Notes 13, no. 1 (2020). http://dx.doi.org/10.1186/s13104-020-4890-z.

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Abstract Objectives Pseudomonas aeruginosa is known as a leading cause of nosocomial infections worldwide. Antimicrobial resistance and biofilm production, as two main virulence factors of P. aeruginosa, are responsible for the persistence of prolonged infections. In this study, antimicrobial susceptibility pattern and phenotypic and genotypic characteristics of biofilm of P. aeruginosa were investigated. Results A total of 80 clinical P. aeruginosa isolates were obtained. Isolates showed resistance to all antibiotics with a rate from 12.5% (n = 10) against amikacin and piperacillin/tazobactam

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R.B., Pal and Upasana Pathak. "PFGE GENOTYPING OF METALLO BETA LACTAMASE PRODUCING PSEUDOMONAS AERUGINOSA." April 14, 2022. https://doi.org/10.5281/zenodo.6526587.

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<strong>Purpose:</strong> Indiscriminate use of antibiotics since a long time has led to the emergence of organisms resistant to many antibiotics. This study was carried out for genotyping of metallo beta lactamase (MBL) producing <em>P.aeruginosa</em>using pulsed field gel electrophoresis (PFGE) and compare the PFGE pattern with the antibiotic susceptibility pattern obtained. <strong>Methods: </strong>In this study the MBL producing <em>P.aeruginosa</em> isolates were subjected to antibiotic sensitivity test against 9 antibiotics and further subjected to PFGE to deter

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