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Dissertations / Theses on the topic 'Geobacter sulfurreducens'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 April 2022

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1

Kagan, Larisa. "Identification of RpoH regulon in geobacter sulfurreducens under heat shock conditions." Diss., [La Jolla] : University of California, San Diego, 2009. http://wwwlib.umi.com/cr/ucsd/fullcit?p1465077.

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Thesis (M.S.)--University of California, San Diego, 2009.<br>Title from first page of PDF file (viewed June 22, 2009). Available via ProQuest Digital Dissertations. Includes bibliographical references (p. 78-80).

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2

Cote, Coy Claudia. "Biocorrosion de l'acier au carbone dans les systèmes d'injection d'eau de l'industrie du pétrole et du gaz : nouveaux modèles expérimentaux issus du terrain." Thesis, Toulouse, INPT, 2013. http://www.theses.fr/2013INPT0038/document.

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L'industrie pétrolière et gazière subie d’importantes pertes économiques en raison de problèmes liés à la corrosion. Parmi ces problèmes, la corrosion induite par les micro-organismes (biocorrosion) fait toujours l’objet de recherche, le mécanisme le plus souvent évoqué et documenté étant lié aux bactéries sulfato-réductrices (BSR). Cependant certaines études ont montré que la biocorrosion pouvait se produire même en absence de BSR dans l'environnement corrosif ; le principal objectif de la thèse était donc de fournir un nouvel éclairage sur la corrosion anaérobie de l'acier au carbone en prop

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3

Champigneux, Pierre. "Comprendre et optimiser les anodes microbiennes grâce aux technologies microsystèmes." Thesis, Toulouse, INPT, 2018. http://www.theses.fr/2018INPT0051/document.

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De multiples micro-organismes ont la capacité de catalyser l’oxydation électrochimique de matières organiques en s’organisant en biofilm à la surface d’anodes. Ce processus est à la base de procédés électro-microbiens très innovants tels que les piles à combustible microbiennes ou les électrolyseurs microbiens. L’interface biofilm/électrode a été l’objet de nombreuses étudesdont les conclusions restent difficiles à démêler en partie du fait de la diversité des paramètres interfaciaux mis en jeu. L’objet de ce travail de thèse est d’exploiter les technologies microsystèmes pour focaliser l’impa

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4

Mehanna, Maha. "Mécanismes de transfert direct en corrosion microbienne des aciers : Application à Geobacter sulfurreducens et à l’hydrogénase de Clostridium acetobutylicum." Phd thesis, Toulouse, INPT, 2009. http://oatao.univ-toulouse.fr/7833/1/mehanna.pdf.

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La corrosion induite par les micro-organismes (CIM) génère des pertes économiques mondiales chiffrées en milliards d’euros par an. Il est communément admis que les bactéries sulfato-réductrices (BSR) jouent un rôle clé dans la CIM anaérobie des aciers. Malgré cette unanimité, les essais en laboratoire peinent à reproduire la corrosion des aciers observées en milieu naturel; bien plus, ils n’expliquent pas quel est l’élément qui déclenche la corrosion, puisque les BSR présentes dans de nombreux environnements naturels n’induisent pas systématiquement de corrosion. L’objectif de ce travail est d

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5

Atkinson, Sally. "Studies of multiheme proteins from the disimilatory metal reducing bacteria Shewanella oneidensis MR-1 and Geobacter sulfurreducens." Thesis, University of Edinburgh, 2009. http://hdl.handle.net/1842/3296.

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Genomic analysis of the dissimilatory metal-reducing bacteria Shewanella oneidensis MR-1 and Geobacter sulfurreducens PCA has shown that both are capable of expressing an unprecedented number of multiheme cytochromes. The isolation and characterisation of two of the multiheme cytochromes from these bacteria is the subject of this thesis. Octaheme tetrathionate reductase (OTR) from Shewanella oneidensis has been previously shown to catalyse the reduction of tetrathionate to thiosulfate (Rothery, 2003). Despite having an amino-acid sequence suggesting the presence of eight standard CXXCH hemeatt

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6

Sievers, Philipp [Verfasser], Diethelm [Akademischer Betreuer] Johannsmann, and Ulrich [Akademischer Betreuer] Kunz. "Untersuchung elektrochemisch aktiver Biofilme von Geobacter Sulfurreducens mit der Schwingquarz-Mikrowaage / Philipp Sievers ; Diethelm Johannsmann, Ulrich Kunz." Clausthal-Zellerfeld : Technische Universität Clausthal, 2020. http://d-nb.info/1231362588/34.

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7

Morgado, Maria Leonor Carvalho. "Structural and functional studies of PpcA: a key protein in the electron transfer pathways of Geobacter sulfurreducens." Doctoral thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/8779.

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Dissertação para obtenção do Grau de Doutor em Bioquímica, ramo de Biotecnologia<br>Geobacter species show an impressive respiratory versatility and can sustain their growth by using insoluble extracellular compounds as terminal electron acceptors. The genome of Geobacter sulfurreducens has been completely sequenced and it revealed an unprecedented number of putative c-type cytochromes, 73 of which with more than one heme binding site. Although numerous electron transfer proteins have been identified, the electron transfer pathways that allow G. sulfurreducens to obtain energy are still far f

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8

Krige, Adolf. "Microbial Fuel cells, applications and biofilm characterization." Licentiate thesis, Luleå tekniska universitet, Kemiteknik, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-73938.

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Since the 1900’s it has been known that microorganisms are capable of generating electrical power through extracellular electron transfer by converting the energy found organic compounds (Potter, 1911). Microbial fuel cells (MFCs) has garnered more attention recently, and have shown promise in several applications, including wastewater treatment (Yakar et al., 2018), bioremediation (Rosenbaum & Franks, 2014), biosensors (ElMekawy et al., 2018) desalination (Zhang et al., 2018) and as an alternative renewable energy source in remote areas (Castro et al., 2014). In MFCs catalytic reactions o

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9

Stefánsdóttir, Lára Kristín. "Microbial fuel cells for organic dye degradation." Thesis, KTH, Skolan för bioteknologi (BIO), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-215020.

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10

Bishop, Michael Edward. "Fe(III) reduction in Hanford sediments and its application to chromium immobilization." Miami University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=miami1438442637.

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11

Qian, Xinlei. "Investigation of iron(III) reduction in Geobacter sulfurreducens." 2009. https://scholarworks.umass.edu/dissertations/AAI3380007.

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Outer membrane cytochromes OmcB and OmcS of Geobacter sulfurreducens are two important components of the respiratory chain for extracellular Fe(III) reduction. OmcS is a loosely bound cell surface protein involved in the reduction of insoluble Fe(III). OmcB is an outer membrane protein and required for insoluble and soluble Fe(III) reduction. The objective of this study was to understand better the mechanism of dissimilatory Fe(III) reduction, focusing on the cell surface proteins by further localization, identification of protein-protein interactions, and biochemical characterization of OmcB

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12

Richter, Lubna V. "Mutational analysis of geopilin function in Geobacter sulfurreducens." 2011. https://scholarworks.umass.edu/dissertations/AAI3465075.

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Geobacter sulfurreducens possesses type IV pili that are considered to be conductive nanowires and a crucial structural element in biofilm formation, enabling electron transfer to insoluble metal oxides in anaerobic sediments and to graphite anodes in microbial fuel cells. The molecular mechanism by which electrons are transferred through the nanowires to the electron acceptor is not fully understood. Prior to the work described in this thesis, the gene (pilA) encoding the structural pilus subunit had been identified, but little was known about the functional translation start codon, the lengt

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13

"Electrochemical Charaterization of Anode-respiring Geobacter sulfurreducens and Geoalkalibacter subterraneus." Master's thesis, 2013. http://hdl.handle.net/2286/R.I.18671.

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abstract: To further the efforts producing energy from more renewable sources, microbial electrochemical cells (MXCs) can utilize anode respiring bacteria (ARB) to couple the oxidation of an organic substrate to the delivery of electrons to the anode. Although ARB such as Geobacter and Shewanella have been well-studied in terms of their microbiology and electrochemistry, much is still unknown about the mechanism of electron transfer to the anode. To this end, this thesis seeks to elucidate the complexities of electron transfer existing in Geobacter sulfurreducens biofilms by employing Electroc

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14

Dantas, Joana Margarida Franco. "Follow the red road of triheme cytochromes in Geobacter sulfurreducens." Master's thesis, 2012. http://hdl.handle.net/10362/13969.

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The bacterium Geobacter sulfurreducens (Gs) is capable of oxidizing a large variety of compounds relaying electrons out of the cytoplasm and across the membrane in a process designated as extracellular electron transfer. The Gs genome was fully sequenced and a family composed by five periplasmic triheme cytochromes c7 (designated PpcA-E) was identified. These cytochromes play an important role in the reduction of extracellular acceptors. They contain approximately 70 amino acids, three heme groups with bis-histidinyl axial coordination, and share between 57 and 77% sequence identity. The trih

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15

Silva, Marta Alexandra Fernandes. "Characterization of novel heme-containing sensor proteins from Geobacter sulfurreducens." Doctoral thesis, 2014. http://hdl.handle.net/10362/14390.

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The focus of this Thesis was the study of the sensor domains of two heme-containing methyl-accepting chemotaxis proteins (MCP) from Geobacter sulfurreducens: GSU0582 and GSU0935. These domains contain one c-type heme, form swapped dimers with a PAS-like fold and are the first examples of a new class of heme sensors. NMR spectroscopy was used to assign the heme and polypeptide signals in both sensors, as a first step to probe conformational changes in the vicinity of the hemes. However, the presence of two conformations in solution impaired the confident assignment of the polypeptide signals.

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16

Teixeira, Liliana Patrícia dos Reis. "Unveiling Geobacter sulfurreducens electron transfer chain: probing the mechanisms of bacterial electricity production." Doctoral thesis, 2020. http://hdl.handle.net/10362/113410.

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Geobacter bacteria have been gaining attention due to their biotechnological and bioremediation applications. Species like G. sulfurreducens are capable of bioenergy production and removal of contaminants from wastewaters. G. sulfurreducens is capable of transferring electrons towards extracellular acceptors, in a process called extracellular electron transfer, and it is also capable of accepting electrons in current consuming biofilms. In order to take full advantage of their applications, it is necessary to understand the mechanisms and the proteins involved in these processes. G. sulfurredu

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17

Mendes, Bruno Filipe Poeiras. "Biochemical characterization of a key cytochrome in respiratory electron transfer pathways from Geobacter sulfurreducens." Master's thesis, 2020. http://hdl.handle.net/10451/51312.

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Tese de mestrado, Bioquímica (Bioquímica) Universidade de Lisboa, Faculdade de Ciências, 2020<br>As bactérias do género Geobacter têm um importante impacto no meio ambiente e potencial para aplicações biotecnológicas (biorremediação de contaminantes orgânicos e inorgânicos, produção de bioenergia e bioeletrónica). Estas bactérias conseguem transferir eletrões para aceitadores finais ou aceitá-los de elétrodos, um processo atualmente explorado na área da produção de bioenergia e eletrossíntese microbiana, respetivamente. Estas aplicações baseiam-se na troca eficiente de eletrões entre a célula

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18

Mehta, Teena. "Evaluation of outer-surface proteins in the reduction of iron(III) oxide in Geobacter sulfurreducens." 2006. https://scholarworks.umass.edu/dissertations/AAI3215892.

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Reduction of Fe(III) in the subsurface environment is of immense environmental significance due to its impact on remediation of toxic metals and organic pollutants. Iron is mostly present in these environments as insoluble Fe(III) oxide. Geobacter are the largest known group of Fe(III) reducing microorganisms in the subsurface environment. Previous studies to understand the mechanism of Fe(III) oxide reduction have demonstrated the need for direct contact. Current study was aimed at identifying the outer surface proteins that are involved in establishing contact, electron transfer and subseque

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19

Qian, Xenlei. "Investigation of Fe(III) Reduction in Geobacter Sulfurreducens Characterization of Outer Surface Associated Electron Transfer Components." 2009. https://scholarworks.umass.edu/open_access_dissertations/116.

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Outer membrane cytochromes OmcB and OmcS of Geobacter sulfurreducens are two important components of the respiratory chain for extracellular Fe(III) reduction. OmcS is a loosely bound cell surface protein involved in the reduction of insoluble Fe(III). OmcB is an outer membrane protein and required for insoluble and soluble Fe(III) reduction. The objective of this study was to understand better the mechanism of dissimilatory Fe(III) reduction, focusing on the cell surface proteins by further localization, identification of protein-protein interactions, and biochemical characterization of OmcB

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20

Kaden, Jan [Verfasser]. "Extrazelluläre Elektronenübertragung in einer syntrophen Kokultur aus Geobacter sulfurreducens und Wolinella succinogenes / vorgelegt von Jan Kaden." 2003. http://d-nb.info/969628471/34.

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21

Fernandes, Ana Paula Nascimento 1978. "Caracterização de um novo citocromo do tipo C que forma "nanofios" de Hemos em Geobacter Sulfurreducens." Master's thesis, 2012. http://hdl.handle.net/10451/8706.

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Tese de mestrado em Bioquímica, apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2012<br>O citocromo dodeca-hemico GSU1996, da bacteria Geobacter sulfurreducens, e uma proteína de estrutura linear, constituída por quatro domínios (A a D) e que actua, putativamente, como “nanofio” molecular, transferindo eletrões entre proteínas periplasmáticas ou para proteínas da membrana externa. Neste trabalho, testou-se a aplicação de uma estratégia modular ao estudo deste novo e complexo citocromo c. Foi realizada a expressão heteróloga em E. coli de cada domínio individual e do f

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22

Bourdakos, Nicholas. "Construction and Characterization of Microbial Fuel Cells Using a Defined Co-culture of G. sulfurreducens and E. coli." Thesis, 2012. http://hdl.handle.net/1807/32530.

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An air cathode, membrane-less microbial fuel cell (MFC) containing a co-culture of Geobacter sulfurreducens and Escherichia coli was constructed and compared to pure culture MFCs of both organisms. The E. coli containing MFCs were unsparged and relied on E. coli for oxygen removal. The pure G. sulfurreducens MFC had a power output of 128 mW/m2, compared to 63 mW/m2 for the co-culture at an early stage and 56 mW/m2 for the late stage co-culture. The limiting current density is 404 mA/m2 for the pure G. sulfurreducens culture, 184 mA/m2 for the early co-culture, and 282 mA/m2 for the late co-cul

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23

Dantas, Joana Margarida Franco. "Characterization of extracellular electron transfer networks in Geobacter sulfurreducens, a key bacterium for bioremediation and bioenergy applications." Doctoral thesis, 2017. http://hdl.handle.net/10362/27867.

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Geobacter bacteria have awakened significantly attention because of their impact on natural environments and biotechnological applications that include the bioremediation of organic and inorganic contaminants, bioenergy production and bioelectronics. In addition to electron transfer towards extracellular terminal acceptors, Geobacter cells can also accept electrons from electrodes, in currentconsuming biofilms, a process that is currently explored in microbial electrosynthesis. These practical applications rely on an efficient transfer of electrons between the cell and its exterior, a process

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24

Antunes, Jorge Manuel Andrade. "Biochemical characterization of the inner membrane cytochrome CbcL: a gate for extracellular electron transfer in Geobacter sulfurreducens." Master's thesis, 2022. http://hdl.handle.net/10362/133794.

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Exoelectrogenic microorganisms are in the spotlight due to their particular respiratory mechanisms and potential application in several biotechnological fields, including bioremediation, bioenergy production and microbial electrosynthesis. All these emergent applications rely on the capability of exoelectrogens to perform extracellular electron transfer, a mechanism that allows the bacteria to transfer electrons to the cell’s exterior. These respiratory pathways encompass different multiheme cytochromes along the inner membrane, periplasmic space, and outer membrane. CbcL from Geobacter sulfur

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25

Lien, Chia-min, and 連家敏. "Effects of quinone moieties on the reductive dissolution of ferric oxides and transformation of carbon tetrachloride in the presence of Geobacter sulfurreducens." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/74705236882433681371.

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碩士<br>國立清華大學<br>生醫工程與環境科學系<br>95<br>The dechlorination of carbon tetrachloride (CT) by biogenic iron species produced from the reductive dissolution of ferrihydrite by Geobacter sulfurreducens in aqueous solutions containing quinone compounds as electron mediators was investigated. The use of quinone compounds in the presence of G. sulfurreducens under iron-reductive conditions can effectively dechlorinate CT. The dechlorination of CT followed pseudo-first-order kinetics and the pseudo-first-order constants (kobs) for 10 �嵱 AQDS, LQ (lawson) and NQ (naphthoquinone) were correspond to 6.5, 5.

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26

Fernandes, Ana Paula Nascimento. "LIVING CAPACITORS: FUNCTIONAL CHARACTERIZATION OF A NOVEL CYTOCHROME ACTING AS A NANOWIRE." Doctoral thesis, 2019. http://hdl.handle.net/10362/91579.

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In an Era where environmental issues are a growing concern, microorganisms that have remarkable features, such as extracellular electron transfer (EET) ability, present major opportunities in diverse biotechnological fields. Geobacter bacteria have shown an extraordinary respiratory flexibility, with its dissimilatory metal reduction ability and EET to electrode surfaces, and numerous c-type cytochromes were pointed as key players. However, the understanding of the mechanisms involved and hence, the advances in practical applications, are still in its early days and it is crucial to move furth

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27

Thrall, Elizabeth Simmons. "Spectroscopic Studies of Abiotic and Biological Nanomaterials: Silver Nanoparticles, Rhodamine 6G Adsorbed on Graphene, and c-Type Cytochromes and Type IV Pili in Geobacter sulfurreducens." Thesis, 2012. https://doi.org/10.7916/D8CF9X66.

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This thesis describes spectroscopic studies of three different systems: silver nanoparticles, the dye molecule rhodamine 6G adsorbed on graphene, and the type IV pili and c-type cytochromes produced by the dissimilatory metal-reducing bacterium Geobacter sulfurreducens. Although these systems are quite different in some ways, they can all be considered examples of nanomaterials. A nanomaterial is generally defined as having at least one dimension below 100 nm in size. Silver nanoparticles, with sub-100 nm size in all dimensions, are examples of zero-dimensional nanomaterials. Graphene, a s

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28

Duldhardt, Ilka [Verfasser]. "Untersuchung des Einflusses organischer Lösungsmittel und umweltrelevanter Schadstoffe auf das Wachstum und die zelluläre Fettsäurezusammensetzung von Thauera aromatica, Geobacter sulfurreducens und Desulfococcus multivorans' / vorgelegt von von Ilka Duldhardt." 2008. http://d-nb.info/99537368X/34.

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29

"Energy and the Environment: Electrochemistry of Electron Transport Pathways in Anode-Respiring Bacteria and Energy Technology and Climate Change in Science Textbooks." Doctoral diss., 2016. http://hdl.handle.net/2286/R.I.38712.

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abstract: The finite supply of current energy production materials has created opportunities for the investigation of alternative energy sources in many fields. One example is the use of microorganisms in bioenergy applications, such as microbial fuel cells. Present in many types of environments, microorganisms with the ability to respire solid electron acceptors have become of increasing relevance to alternative energy and wastewater treatment research. In this dissertation, several aspects of anode respiration are investigated, with the goal of increasing the limited understanding of the

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