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Journal articles on the topic 'Gerbera Multiplication Medium'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Bhatia, R., K. P. Singh, and M. C. Singh. "In vitro mass multiplication of gerbera (Gerbera jamesonii) using capitulum explant." Indian Journal of Agricultural Sciences 82, no. 9 (2012): 768–74. http://dx.doi.org/10.56093/ijas.v82i9.23830.

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An experiment was conducted to develop highly efficient and economically viable protocol for rapid in vitro multiplication of gerbera cv. cabana using capitulum as explant during 2005–07. Among the two stages initially used for culture initiation, capitulum explants cultured at immature stage responded better than the mature stage. Best establishment of immature capitulum explants was obtained on modified MS medium supplemented with 10 mg/l BAP and 1 mg/l IAA. The type and concentration of cytokinin also showed profound effect on proliferation. Better proliferation of quality shoots was obtain

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2

Couto, Tarcisio Rangel do, João Sebastião de Paula Araujo, and João Paulo de Lima Aguilar. "Balanço hormonal auxina/citocinica para multiplicação in vitro de genótipos de gérbera." Revista Agraria Academica 4, no. 1 (2021): 119–34. http://dx.doi.org/10.32406/v4n12021/119-134/agrariacad.

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Gerbera is used as a cut flower and has gained popularity as ornamental flower and great demand in the world market for ornamental plants. Micropropagation is used to meet the demand for commercial planting material. The objective was to evaluate the BAP and ANA phytoregulators effect gerberas in vitro multiplication. The explants were inoculated in MS culture medium containing different concentrations of BAP (0.0; 2.22; 4.44; 8.88 and 17.76 µmol L-1) and ANA (0.0; 1.34; 2.68 and 5.36 µmol L-1). After eight weeks, the number of shoots formed in each explant and the average length of the shoots

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3

Phan, Thi Thu Hien, Thi Kim Anh Nguyen, Thi Tuyet Cham Le, and Thi Thuy Hang Vu. "In vitro rapid multiplication of two ancient gerbera varieties (Gerbera jamesonii Bolus)." Ministry of Science and Technology, Vietnam 66, no. 2 (2024): 66–74. http://dx.doi.org/10.31276/vjst.66(2).66-74.

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Gerbera buds are double sterilised, the clean sample rate is up to 90% (ancient yellow gerbera daisies) and 92% (ancient pink gerbera daisies). With the medium supplemented with 1.5 mg/l BAP and 2 mg/l TDZ, the yellow gerbera daisy variety obtained a regeneration rate of 52.17%, the number of regenerated buds was 5 buds/sample. Ancient pink gerbera daisies have a regeneration rate of 53.85%, the number of regenerated buds was 6 buds/sample. When using 1.5 mg/l IBA combined with 2 mg/l BA, the gerbera daisies obtained a bud formation rate of 44.45% with an average number of 5 buds/sample; ancie

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4

Shlahi, Sattar Abdullah, Duha Mysire Majeed, and Salah Mohammed Hasan. "In vitro propagation of Gerbera (Gerbera jamesonii Bolus)." Journal of Biotechnology Research Center 7, no. 1 (2013): 50–58. http://dx.doi.org/10.24126/jobrc.2013.7.1.245.

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Gerbera plant Gerbera jamesonii is classified according to the flower colors to four strains: white, yellow, pink and purple. Capitulum and scape explants were tested on MS medium in half or full salts strength, supplemented with different combinations of plant growth regulators cytokinins kintin (Kin) and benzel adinine (BA), auxin indolacitic acid (IAA). Results revealed that the capitulum showed better response to shoot formation 64.13% whereas the scape did not show response. Yellow flowers showed higher response in shoot formation 37.5% than other strains. growth regulators combination BA

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5

Akter, Nasrin, Selim Sarkar, Naimul Hasan, Shaikh Mizanur Rahman, and Md Abdur Rauf Sarkar. "Development of In vitro Mass Propagation Protocol for Gerbera (Gerbera jamesonii Bolus) var. Orange." Plant Tissue Culture and Biotechnology 32, no. 2 (2022): 217–26. http://dx.doi.org/10.3329/ptcb.v32i2.63555.

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An in vitro mass propagation protocol was developed for Gerbera (Gerbera jamesonii Bolus) var. orange using flower bud explants. The young (7 days) explants were found to be suitable for culture and highest (100%) callus induction was obtained in MS medium supplemented with 5.0 mg/l BAP with 1.0 mg/l NAA. The maximum shoot regeneration (95%) and the number of shoots (10) were observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA. The highest shoot multiplication frequency (95%) and the number of shoots (60) were obtained in MS medium supplemented with only 2.0 mg/l BAP. The we

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6

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, and Snehasis Jana. "Physicochemical and Spectroscopic Characterization of Biofield Energy Treated Gerbera Multiplication Medium." Plant 3, no. 6 (2015): 57–63. https://doi.org/10.5281/zenodo.186725.

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The micropropagation technique is used for Gerbera species due to their high demand all over the world as the decorative potted plants and cut flowers. The present study was done to investigate the impact of biofield energy treatment on the physicochemical properties of gerbera multiplication medium. A part of the sample was treated with Mr. Trivedi’s biofield energy, and the other part was kept as untreated and termed as the control sample. Both the parts were subsequently analysed for their physical, thermal and spectral properties using X-ray diffraction (XRD), particle size analysis,

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7

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, and Snehasis Jana. "Physicochemical and Spectroscopic Characterization of Biofield Energy Treated Gerbera Multiplication Medium." Plant 3, no. 6 (2015): 57–63. https://doi.org/10.11648/j.plant.20150306.11.

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The micropropagation technique is used for Gerbera species due to their high demand all over the world as the decorative potted plants and cut flowers. The present study was done to investigate the impact of biofield energy treatment on the physicochemical properties of gerbera multiplication medium. A part of the sample was treated with Mr. Trivedi’s biofield energy, and the other part was kept as untreated and termed as the control sample. Both the parts were subsequently analysed for their physical, thermal and spectral properties using X-ray diffraction (XRD), particle size analysis,

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8

Kumar Trivedi, Mahendra. "Physicochemical and Spectroscopic Characterization of Biofield Energy Treated Gerbera Multiplication Medium." Plant 3, no. 6 (2015): 57. http://dx.doi.org/10.11648/j.plant.20150306.11.

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9

Cockrel, Angela D., G. L. McDaniel, and E. T. Graham. "In Vitro Propagation of Florists’ Cineraria." HortScience 21, no. 1 (1986): 139–40. http://dx.doi.org/10.21273/hortsci.21.1.139.

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Abstract Tissue culture propagation of Senecio cruentus DC. ‘Hansa’ was achieved from shoot tips derived from selected seedlings germinated in vitro. Shoot multiplication was best accomplished on Murashige and Skoog (MS) gerbera medium supplemented with 2.8 μM IAA. Maximum root formation occurred on MS modified medium with reduced salt levels and 1.3 μM NAA. Rooted shoots were established successfully in a soilless medium under high humidity. Chemical names used: 1H-indole-3-acetic aid (IAA); 1-naphthaleneacetic acid (NAA).

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10

MAHINDRAKAR, AAKANKSHA, RAJIV KUMAR, C. ASWATH, and P. M. MUNIKRISHNAPPA. "Callusing and regeneration response of in vitro derived leaf explants of gerbera (Gerbera jamesonii)." Indian Journal of Agricultural Sciences 88, no. 6 (2018): 860–64. http://dx.doi.org/10.56093/ijas.v88i6.80584.

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Gerbera (Gerbera jamesonii Bolus ex. Hookes F.) is one of the most popular commercial flowers worldwide used both as cut flower and potted plant. Conventionally, it is propagated through vegetative means such as divisions of clump which is very slow process and not commercially viable. Rapid multiplication of elite hybrids/genotypes with improved quality traits, has been achieved by using both direct and indirect tissue culture methods. Gerbera hybrid seeds obtained by half-sib mating were raised in half strength MS medium. The earliest days for seed germination(2.00), first leaf development (

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11

WINARTO, Budi, Kurnia YUNIARTO, and Rudy SOEHENDI. "Young capitulum as important explant in in vitro mass propagation of gerbera (Gerbera jamesonii)." Notulae Scientia Biologicae 12, no. 2 (2020): 264–76. http://dx.doi.org/10.15835/nsb12210712.

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A new route of in vitro propagation of gerbera selected clones was successfully established using young capitula in tight buds and buds that were started to unfold stage as explant source. The one-fourth pieces of young capitula of tight flower stage and half-strength MS medium containing 0.25 mg/l BAP was the suitable for initiation and produced higher number of shoots per explant up to 3.8 shoots. The results were improved by culturing the one-fourth piece of 01.092 capitulums on MS medium fortified by 0.2 mg/l BAP and 0.02 mg/l NAA producing the highest shoot formation up to 8.5 shoots per

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12

Soczek, U., and M. Hempel. "THE INFLUENCE OF SOME ORGANIC MEDIUM COMPOUNDS ON MULTIPLICATION OF GERBERA IN VITRO." Acta Horticulturae, no. 226 (June 1988): 643–46. http://dx.doi.org/10.17660/actahortic.1988.226.89.

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13

Cioć, Monika, and Bożena Pawłowska. "Leaf Response to Different Light Spectrum Compositions during Micropropagation of Gerbera Axillary Shoots." Agronomy 10, no. 11 (2020): 1832. http://dx.doi.org/10.3390/agronomy10111832.

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The experiment evaluated the influence of different light qualities and 6-benzyladenine (BA) concentration in a medium on the leaf response of multiplied Gerbera jamesonii Bolus ex Hook. f.‘Big Apple’ shoots. Three different light-emitting diode (LED) spectra—100% blue (B), 100% red (R) and red and blue mixture (7:3, RB)—were used, and a fluorescent lamp was used as a control (Fl). Concentrations of BA in Murashige and Skoog (MS) medium were 1, 2.5 and 5 µM. Leaves developed under 100% blue light had a lower frequency of stomata and a smaller area as compared with those from plants exposed to

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14

Dubuc-Lebreux, M. A., та J. Vieth. "Effets des rayons γ 60Co sur des tigelles de Gerbera jamesonii irradiées in vitro". Canadian Journal of Botany 65, № 2 (1987): 261–67. http://dx.doi.org/10.1139/b87-037.

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The aim of this work was to determine the direct inhibitory effects of 60Co γ-rays on plantlets and to control them in a future program of experimental mutagenesis. Gerbera jamesonii plantlets were irradiated in vitro using two sources of 60Co γ-rays. The following variables were tested: dose rate, total dose, irradiation of the growth medium, time interval between the exposure to γ-rays and the subdivision and transfer to fresh medium, and dose fractionation. These variables were evaluated using regeneration rate of new shoots, callus production, and plantlet growth rate (fresh weight). The o

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15

WINARTO, Budi, Kurnia YUNIARTO, and Dan M. WEGADARA. "Axillary Shoots Derived from Thin Cell Layer and Adenine Sulphate Application in in vitro Mass Propagation of Gerbera [Gerbera jamesonii (H. Bolus ex Bolus f.)]." Notulae Scientia Biologicae 11, no. 1 (2019): 63–76. http://dx.doi.org/10.15835/nsb11110351.

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A new route of in vitro mass propagation protocol of Gerbera jamesonii (H. Bolus ex Bolus f.) derived from application of thin cell layers (TCL) and adenine sulphate (AS) was successfully developed and established. Shoot tip explants and half-strength MS medium containing 0.25 mg/l N6-benzylaminopurine (BAP), 20 g/l sucrose and 7 g/l Swallow agar were used as explant source and basic medium. Different TCL of transversal TCL (tTCL) and longitudinal TCL (lTCL) in four slicing positions of 1, 2, 3 and 4; varieties and clones i.e. G. jamesonii ‘Black Jack’, ‘Carambole’, ‘Nuance’, ‘Violente’, 01.09

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16

Mohamed, Seifeldin Ali, and Mustafa Ercan Özzambak. "Effect of Cultivar and Gelling Agent on In Vitro Multiplication of Gerbera (Gerbera jamesonii Bolus) Shoots." University of Khartoum Journal of Agricultural Sciences 24, no. 1 (2023). http://dx.doi.org/10.53332/uofkjas.v24i1.1236.

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An experiment was conducted to study the effect of cultivar andconcentration of agar and gelrite on in vitro multiplication of gerbera(G.jamesonii) shoots. Sterile in vitro growing shoots of the gerbera cultivarsAmeretto, Red Bull, Ruby Red, Yellowish green and Crillo were placed inglass jars containing nutrient media with different concentrations of agar andgelrite. There was a significant interaction between the gelling agents’concentrations and the gerbera cultivars. There was a decrease in themultiplication and vitrification rates with the increase of the concentration ofthe different gell

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17

Shaheen, Aaqib, Yaser Hassan Dewir, Mafatlal Kher, Majid Khan, Antar Nasr El-Banna, and Ahmed Alaizari. "Synergistic effect of benzylaminopurine and meta-Topolin combination for micropropagation of gerbera ‘Pink Melody’." Ciência e Agrotecnologia 46 (2022). http://dx.doi.org/10.1590/1413-7054202246017521.

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ABSTRACT Gerbera (Gerbera jamesonii Bolus ex Hook. F.; Asteraceae), is one of the most economically important ornamental plants due to its aesthetic value. In the present study, we established a micropropagation method for the large-scale production of quality planting material of gerbera ‘Pink Melody’. Eighty-six percent of the capitulum explants produced adventitious shoots (15.44 ± 0.34 shoots per capitulum) on Murashige and Skoogs (MS) medium containing 2 mg L−1 6-benzylaminopurine (BAP) after six weeks of incubation. The highest shoot multiplication rate (17 shoots per explant) was obtain

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18

Mahendra, Trivedi, Branton Alice, Trivedi Dahryn, and Nayak Gopal. "Physicochemical and Spectroscopic Characterization of Biofield Energy Treated Gerbera Multiplication Medium." Science Publishing Group, November 9, 2015. https://doi.org/10.5281/zenodo.813617.

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Abstract:

The micropropagation technique is used for Gerbera species due to their high demand all over the world as the decorative potted plants and cut flowers. The present study was done to investigate the impact of biofield energy treatment on the physicochemical properties of gerbera multiplication medium. A part of the sample was treated with Mr. Trivedi's biofield energy, and the other part was kept as untreated and termed as the control sample. Both the parts were subsequently analysed for their physical, thermal and spectral properties using X-ray diffraction (XRD), particle size analysis, surfa

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