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1

David, Andrew. "Germination Percentage and Germination Speed of European Larch (Larix decidua Mill.) Seed After Prolonged Storage." Northern Journal of Applied Forestry 19, no. 4 (2002): 168–70. http://dx.doi.org/10.1093/njaf/19.4.168.

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Abstract Thirty European larch seedlots stored in plastic, screw-capped containers at 4°C for 3 to 24 yr were tested for their viability. The average germination percentage was 26.7% per seedlot. Fifty percent of all germinating seeds germinated by the 20th day after sowing and 90% had germinated by the 26th day. Correlation analysis indicated there was a significant negative association between years in storage and germination percentage and a significant positive association between years of storage and the number of days required to reach 15% germination. Although one seedlot germinated no seeds after 10 yr in storage, 21 of 30 seedlots, ranging from 3 to 24 yr in storage, maintained a minimum germination percentage of 20%.
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2

Harp, Derald A., Michael Wade, D. Frank Gilman, and John Calahan. "Hormonal Effects on the Germination Response of Chasmanthium latifolium." HortScience 30, no. 4 (1995): 836D—836. http://dx.doi.org/10.21273/hortsci.30.4.836d.

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Caryopses of Chasmanthium latifolium removed for the florets, treated with solutions containing 0.02 M KNO3, 0.5 mM GA3, and/or 0.1 mM kinetin, placed in germination chambers at alternating temperatures of 15/30C, and percentage germination was checked at 7, 14, and 21 days. Treatments with kinetin and/or KNO3 significantly increased germination percentage over other treatment combinations. The maximum germination percentage for Chasmanthium latifolium was achieved by removing the caryopsis from the floret, treating the caryopsis with 0.02 M KNO3, and germinating for 14 days at alternating temperatures of 15/30C.
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3

Carpenter, William J., and Joseph F. Boucher. "Germination and Storage of Vinca Seed Is Influenced by Light, Temperature, and Relative Humidity." HortScience 27, no. 9 (1992): 993–96. http://dx.doi.org/10.21273/hortsci.27.9.993.

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Light, temperature, relative humidity (RH), and GA3 affect vinca [Catharanthus roseus (L.) G. Don] seed storage and/or germination. GA3 failed to increase the germination percentage in darkness but significantly increased the percentage in continuous light. Similarly, GA3 treatment reduced both the number of days required to achieve 50% of the final germination percentage (T50) and the span between 10% and 90% germination (T90 — T10) for seeds in light, but not in darkness. Germination percentages were maximal and about equal at 25, 30, or 35C in darkness; germination was lowest below 25C. Germination T50 and T90 — T10 required the fewest days between 25 and 35C. Reducing seed moisture from 9.9% to 3.9% increased the T50 from 2.4 to 3.0 days but failed to change germination percentages. Germination percentage declined linearly as seed storage temperatures were reduced from 5 to — 20C, whereas days to T50 increased. Seed storage for 12 months without reduction in germination percentage was possible at 5C and 11%, 33%, or 52% RH, but storage at 75% or 95% RH for periods exceeding 1 month reduced germination. Seeds stored at 33% or 52% RH required fewer days to T50 than did seeds stored at 11%, 75%, or 95% RH. Chemical name used: gibberellic acid (GA3).
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4

Tabatabaei, S. A. "The Changes of Germination Characteristics and Enzyme Activity of Barley Seeds under Accelerated Aging." Cercetari Agronomice in Moldova 48, no. 2 (2015): 61–67. http://dx.doi.org/10.1515/cerce-2015-0030.

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Abstract Seed aging is the main problem of seed storage. Changes of enzyme activity and reduction of seedling growth are consequence of seed deterioration. An experiment was conducted to evaluate the effects of accelerated aging on germination indexes and enzyme activity of barley seeds. Seeds were incubated in closed plastic boxes for the accelerated aging treatments at 41°C. Three accelerate aging regimes were performed by placing seeds at 41°C and relative humidity (RH) of 90-100 % for 0, 4 and 8 day periods. Our results showed that increasing aging duration resulted higher reduction in germination percentage, germination index, mean time to germination, normal seedling percentage, catalase and ascorbate peroxidase. The highest germination percentage, germination index, normal seedling percentage and enzyme activity were achieved in control conditions (0 day of aging). Under aging conditions, germination percentage, means time to germination, germination index, normal seedling percentage and enzyme activity decrease significantly. Also, our results indicated that seed aging is related to decrease of enzymes and may contribute to low germination efficiency. The general decreases in enzyme activity in the seed lowers the respiratory capacity, which in turn lowers both the energy (ATP) and assimilates supply of the germinating seed, also decrease in antioxidant enzymes is linked to an increased accelerated ageing and decreased germination characteristics. Subsequently, proposed a positive relationship between antioxidant enzyme capacity and the vigour of the seed
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5

Bradford, Kent J., Daniel Côme, and Françoise Corbineau. "Quantifying the oxygen sensitivity of seed germination using a population-based threshold model." Seed Science Research 17, no. 1 (2007): 33–43. http://dx.doi.org/10.1017/s0960258507657389.

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AbstractSeeds vary widely in the sensitivity of germination to oxygen (O2) partial pressure, depending upon the species, temperature, dormancy state and physiological status of the seeds. Most analyses of the O2 sensitivity of germination have focused on final germination percentages and estimated the O2 percentage in air that is required to reduce germination to a given percentage (usually 50%). In contrast, we have applied a population-based threshold model utilizing time courses of germination to quantify three parameters related to seed germination sensitivity to O2 availability: the median base (or threshold) O2 percentage, the standard deviation of O2 thresholds among seeds in the population, and an oxygen–time constant that relates O2 percentage to germination timing. The model fits germination responses accurately across a wide range of O2 concentrations. The response to O2 was logarithmic in all cases, with the O2 percentage required for 50% germination ranging from 21% to as low as 0.005%, depending upon the species, the temperature and the seed dormancy level. Modelling indicated that some seeds can adapt to low O2 percentages and shift their thresholds to lower values over time. Lower temperatures decreased the minimum O2 threshold, as did after-ripening. Seed priming generally reduced the oxygen–time constant and increased the standard deviation of germination responses, but had relatively little effect on the O2 sensitivity per se. The population-based threshold model can be used to quantify the O2 sensitivity of seed germination and to predict germination rates and percentages when O2 availability is limiting.
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6

Liu, Mengzhou, Ning Qiao, Bing Zhang, et al. "Differential responses of the seed germination of three functional groups to low temperature and darkness in a typical steppe, Northern China." PeerJ 10 (December 1, 2022): e14485. http://dx.doi.org/10.7717/peerj.14485.

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Seed germination is a key stage in the life history of plants, which has a crucial effect on plant community structure. Climate change has substantially altered the surface soil temperature and light availability, which can affect seed germination. However, whether the seed germination of different functional groups is affected by the interactions of light and temperature remains unclear. Under laboratory conditions, we examined the effects of low temperature and darkness, as well as their interaction, on the seed germination of 16 species belonging to three plant functional groups (annual and biennials, perennial grasses, and perennial forbs) in a typical steppe, Northern China. We found that low temperature had a significant negative effect on seed germination of all species. Low temperature significantly decreased the final germination percentage and germinative force of the three plant functional groups, and the germination duration of perennial grasses. Darkness significantly decreased the germinative force of perennial forbs and total seeds, and the germination duration of perennial grasses. The interactive effects of light and temperature on the seed final germination percentage and germinative force of perennial grass indicated that darkness strengthened the inhibitory effect of low temperature on the seed germination of the grass functional group. Our study indicate that the seed germination of different plant functional groups varied greatly in response to changing environmental conditions. Our results suggest that future climate change could alter the regeneration and species composition of plant communities through changing seed germination.
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7

Zargar, Tahoora Batool, Faiza Ashraf, and Szilvia Veres. "Peg- Induced Drought Stress Effects on Spinach Germination Parameters." Review on Agriculture and Rural Development 10, no. 1-2 (2021): 126–32. http://dx.doi.org/10.14232/rard.2021.1-2.126-132.

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Exposure to the drought stress reduces germination rate and seedling's growth with significant variations from crop to crop. An unavoidable consequence of drought exposure is the generation of reactive oxygen species. They can be extremely reactive with several cellular constituents such as proteins, lipids, and nucleic acids. Spinach (Spinacia oleracea L.) is one of the most consumed vegetable species, rich in vitamins, such as vitamin C, and minerals, which are essential for human health. Besides, spinach contains large amounts of bioactive molecules such as glucuronic acid derivatives of flavonoids and p-coumaric acid derivatives that exhibit strong antioxidant activity Osmotic solutions of polyethylene glycol (PEG) are commonly used to control water potential in seed germination studies. PEG reduces the water potential of osmotic solutions but may limit oxygen availability to germinating seeds. The purpose of this study was to determine whether seed immersion in PEG solution had a detrimental effect on total Germination percentage, Relativized percentage, Mean germination time, Mean germination rate, Coefficient of variation of germination time, Coefficient of the velocity of germination, Germination index, Uncertainty of germination process, Synchronization index, Time to 10% germination, Time to 25% germination, Time to 50% germination, Time to 75% germination, Time to 90% germination, Time from 10 to 90% germination, Time from 25 to 75% germination, Mean daily germination Percent, Peak value for germination, Germination value. A controlled experiment was conducted to investigate the effects of drought stress, induced by PEG, on these germination parameters in Spinach. For control, seeds were treated with nutrient solution, and 2.5% PEG was used to induce drought stress. The seeds were germinated geotropically between moisten filter papers. Each roll contained 30 seeds. Overall germination parameters showed a decrease as compared to control. The germination percentage of control seeds of Spinach oleracea L. was 79% while those treated with PEG were only 63%. Similarly, the relativized percentage decrease was observed in the case of PEG-treated seeds. Relativized percentage of control seeds was 91% as treated seeds were only 73%. Both showed a significant decrease as compared to the control.
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8

Mohammed, Abdullah. "Effect of gibberellic acid on germination and seedling growth of Soybean (Glycine max L. Merrill)." Bionatura 8, no. 2 (2023): 1–6. http://dx.doi.org/10.21931/rb/2023.08.02.41.

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The germination percentage of Soybeans is susceptible and affected by many external and internal factors. Screening of three varieties of soybeans (Shimaa, Industrial2, and M103) during laboratory germination and field emergence was conducted to identify varieties with superior performance under four concentrations of gibberellic acid (0, 75, 150, 225 ppm). Experiments were conducted in Field Crops Dept./College of Agriculture/Tikrit University. Results showed significant differences between gibberellic acid concentrations, varieties, and interactions for all studied traits. Seeds treated with a concentration of (150 ppm) were superior in laboratory germination and field emergence percentages, averaging 92.58% and 59.08%, respectively. Varieties of Shimaa and M103 were superior in giving the highest germination percentage in the laboratory, 87.5% and 87.94%, respectively. The Shimaa variety outperformed, giving the highest field emergence percentage, 58.00%. Interaction of Shaima x150 ppm (GA3) showed the highest laboratory germination percentage and highest field emergence percentage, 96.75% and 63.25%, respectively. These Results indicate that the use of gibberellic acid significantly increased the germination rate of soybean seeds and field emergence rate due to the role of this hormone in stimulating the synthesis of enzymes necessary for germination and seedling vigor. Keywords: Soybean; GA3; Seed germination percentage; Field emergence percentage.
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9

Hussain, Afrida, and Dhruva Jha. "Seed Germinatio n Improvement in Two Threatened Medicinal Plants." Current Agriculture Research Journal 2, no. 2 (2014): 131–36. http://dx.doi.org/10.12944/carj.2.2.10.

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Rauvolfia serpentina (L.) Benth. ex Kurz. and R. tetraphylla L. are threatened because of extensive utilization for their wide ranging medicinal applications. The seed mediated propagation is unsatisfactory due to dormancy and poor germination percentage. This however, decelerates the conservation strategy of these species. Thus, efforts were made in this study to break dormancy and improve germination of seeds of the two species for boosting conservation. The viability of R. serpentina and R. tetraphylla were found to be 67% and 82% respectively. Germination percentage of treated R. serpentina seeds showed improved germination percentage of 34.94% (H2O2) and 48.65% (GA3) over control (11.27%). The germination percentages of treated R. tetraphylla seeds were improved to 52.70% (KNO3) and 56.66% (GA3) as compared to untreated seeds (31.26%). Temperature also influenced the germination percentage and the highest germination percentage was obtained at 35oC and 30oC respectively in R. serpentina and R. tetraphylla. Results of the treatments indicate the presence of coat induced and non-deep physiological dormancy in both these species. The datas were statistically analyzed by analysis of variance (P<0.01).
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10

Gbèhounou, Gualbert, Arnold H. Pieterse, and Jos A. C. Verkleij. "Longevity ofStrigaseeds reconsidered: results of a field study on purple witchweed (Striga hermonthica) in Bénin." Weed Science 51, no. 6 (2003): 940–46. http://dx.doi.org/10.1614/ws03-0221r1.

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Germination and longevity of purple witchweed seeds stored in nylon gauze bags in the soil in situ were tested in northern Bénin over a 2-yr period, covering the rainy seasons in 1994 and 1995. The seeds were collected at Ina Station in November 1993 from corn and sorghum fields. It appeared that germination percentages of the seeds, which were stimulated by GR24 to germinate, as well as their viability according to a tetrazolium test, decreased steadily in wet soil. During the 1994 rainy season, germination percentage of seeds, which reached maximum values of 30 to 74%, decreased to values of 11 to 17%. During the 1995 rainy season, the number of germinating seeds decreased further, and at the end of this season the germination percentage approached zero. Seed viability also decreased in line with the decrease in germination. In addition to the study on longevity under field conditions, seeds also were exposed to various water regimens in pots. In the pot experiment, purple witchweed seed viability and germination declined in moist soil treatments. The dying-off process observed contradicts the common opinion on longevity ofStrigaseeds in their natural environment. “Wet dormancy” was not observed in the course of the rainy season.
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11

Aydin, Ali Cem, Alper Ahmet Özbey, Mehmet Çalikoğlu, and Bohdan Konôpka. "A new method for early prediction of Turkish red pine (Pinus brutia Ten.) germination percentage." Central European Forestry Journal 69, no. 1 (2023): 38–48. http://dx.doi.org/10.2478/forj-2022-0017.

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Abstract This study was carried out to find a method to determine the seed germination ability (percentage) in the Turkish red pine (Pinus brutia Ten.) quickly and with high reliability. For this purpose, 82 seed lots of Turkish red pine were used, collected from different seed stands and orchards in different years. Since none of the seeds were folded, the germination ability was evaluated in a period between the 7th to 28th days. The seeds were divided into 4 groups (20–39.9%, 40–59.9%, 60–79.9%, 80–100%). Germination Percentage Index (GPI) are expressed with the mid-value of the related interval (e.g. GPI_30: germination ability between 20–39.9%). Four different germination percentage intervals describing the germination percentage was fitted with 11 different regression models. The best fit of these models was determined by 7 fitness measures. As a result, the current germination percentage increment reached the highest values on the 13th, 13th, 14th, and 13th day for the indexes of 90, 70, 50, and 30, respectively. Within this study, it has been revealed that germination percentages in Turkish red pine seed lots can be predicted 7 to 10 days in advance.
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12

Cave, Robyn L., Colin J. Birch, Graeme L. Hammer, John E. Erwin, and Margaret E. Johnston. "Cardinal Temperatures and Thermal Time for Seed Germination of Brunonia australis (Goodeniaceae) and Calandrinia sp. (Portulacaceae)." HortScience 46, no. 5 (2011): 753–58. http://dx.doi.org/10.21273/hortsci.46.5.753.

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Seed germination of Brunonia australis Sm. ex R.Br. and Calandrinia sp. (Mt. Clere: not yet fully classified) was investigated using a thermogradient plate set at different constant temperatures to determine seed propagation requirements of these potential floriculture species. Germination responses were tested at 3, 7, 11, 15, 18, 22, 25, 29, 34, and 38 °C. Germination data were modeled using the cumulative distribution function of the inverse normal, which provides information on lag, rate, and maximum seed germination for each temperature regime. To determine cardinal temperatures, the reciprocal time to median germination (1/t50) and percentage germination per day were calculated and regressed against temperature. Base temperature estimates for B. australis were 4.9 and 5.5 °C and optimum temperatures were 21.4 and 21.9 °C, whereas maximum temperatures were 35.9 and 103.5 °C, with the latter being clearly overestimated using the 1/t50 index. Base temperatures for Calandrinia sp. were 5.8 and 7.9 °C, whereas optimum and maximum temperature estimates of 22.5 and 42.7 °C, respectively, were reported using the percentage germination per day index. Maximum seed germination of 0.8 to 0.9, expressed as the probability of a seed germinating, occurred at 11 to 25 °C for B. australis, whereas maximum germination for Calandrinia sp. was 0.5 to 0.7 at 18 to 25 °C. Thermal time, the accumulation of daily mean temperate above a base temperature, was calculated for different germination percentages. Estimates of thermal time (°Cd) for 50% seed germination were 54 and 90 °Cd for B. australis and Calandrinia sp., respectively.
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13

Chaivivatrakul, Supawadee. "Automatic Assessment of Seed Germination Percentage." Engineering Journal 24, no. 4 (2020): 85–96. http://dx.doi.org/10.4186/ej.2020.24.4.85.

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14

Cruz, Oscar, Juan García-Duro, Mercedes Casal, and Otilia Reyes. "Can the mother plant age of Acacia melanoxylon (Leguminosae) modulate the germinative response to fire?" Australian Journal of Botany 65, no. 7 (2017): 593. http://dx.doi.org/10.1071/bt17083.

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Acacia melanoxylon R.Br. (Leguminosae) is one of the most widely distributed tree species in the world. It has significant commercial use but can be a danger to natural ecosystems as an invader species. For these reasons it is necessary to have a deep knowledge about its germinative response to fire and the role the mother plant age plays in that response. In this work we assessed the effects of the interaction of the mother plant age with the main fire agents (smoke, charcoal, ash and heat) on the germinative response of A. melanoxylon. To reach this aim, germination tests were conducted with seeds from young, intermediate and mature mother plants, with 20 treatments each applied. Results show that fire can act as a great stimulator of the germination of A. melanoxylon but it can also strongly inhibit it. The mother plant age does not modify the final germination values, but it influences the time in which the first germinations occur after fire. This trait can be more important than the final germination percentage when looking at the success of post-fire regeneration. These results have important implications for A. melanoxylon management, for both species control and ecological restoration purposes.
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15

Bajwa*, Vikramjit S., and Curt Rom. "Effects of Osmotic Agents on Apple Pollen Grain Germination." HortScience 39, no. 4 (2004): 762B—762. http://dx.doi.org/10.21273/hortsci.39.4.762b.

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Osmotic agents used to prevent apple pollen grain germination were studied in vitro by applying 10 μL of solutions to germinating apple pollen on germinating and growth media. Seven concentrations (0%, 0.25%, 0.5%, 1%, 2%, 5% and 10%) of the solution were prepared for each chemical and the characteristics of pH, EC, and osmotic potential were measured. Apple pollen was dispersed onto the media in petri dishes. Micro drops of solution were then applied to marked areas. Dishes were then placed in germination cabinets at 25 °C. Cumulative percentage pollen germination was calculated 4, 8, 12, and 24 h after treatment by microscopic observation. Generally, the cumulative percentage pollen germination decreased asymptotically with increasing chemical concentration. The most effective chemicals for restricting pollen germination and growth were CuSO4 (0.25%), CH3 COOH (0.25%), CaCl2 (10%), K2 S2 O5 (0.25%), Methyl Jasmonate (2%). The effect of these chemicals has also been tested on pistil viability both in vitro and on excised limbs.
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16

Brasileiro, Beatriz Gonçalves, Denise Cunha F. S. Dias, Vicente Wagner Dias Casali, Maria Carmen Bhering, and Paulo Roberto Cecon. "Effects of temperature and pre-germinative treatments on seed germination of Talinum triangulare (Jacq. ) willd (Portulacaceae)." Revista Brasileira de Sementes 32, no. 4 (2010): 151–57. http://dx.doi.org/10.1590/s0101-31222010000400017.

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The objective of this study was to evaluate the effects of temperature, substrate and pre-germinative treatments on T. triangulare seeds. Four temperatures (constant 20, 25, 30 °C and alternate 20-30 °C) and two types of seeding (on paper and between paper), with light, were evaluated. The pre-germinative treatments evaluated included: immersion in water (24 hours), immersion in 6% hypochlorite solution (1 hour), immersion in 0.2% potassium nitrate solution (24 hours), immersion in 0.05% gibberellin solution (24 hours) and the control (untreated seeds). The highest germination percentage was observed at the alternate temperatures of 20-30 ºC, but with no significant difference between the substrates at this temperature. Soaking seeds in KNO3 gave the highest percentage germination and germination speed index (GSI), which differed from the other treatments except for soaking in water. Pre-soaking of T. triangulare seeds favors germination and may be done only in pure water, resulting in a more rapid and uniform germination.
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17

Gül, Volkan, Burcu Seckın Dınler, Fırat Sefaoğlu, Hatice Çetinkaya, and Fatma Nur Koç. "The Effect of Pre-Applied Titanium Dioxide Nanoparticles on Germination in Carthamus tinctorius L. Varieties." Journal of Agricultural Production 5, no. 1 (2024): 41–49. http://dx.doi.org/10.56430/japro.1436131.

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In the present study, to promote sustainable nano-farming, the apparent effects of different concentrations (0, 100, 200, 300, 400, 500 ppm) of titanium dioxide nanoparticle (TiO2NPs) solutions on the germination percentage, index and duration of seeds belonging to Balcı, Dincer, Hasankendi, Koc, Olas, and Zirkon safflower varieties were investigated. Moreover, scanning electron microscopy (SEM) was employed to analyze TiO2NPs in germinated safflower varieties. Germination performance was TiO2NPs concentration and variety depended. It was determined that the seed samples displayed different responses to TiO2NPs concentrations; germination percentages were between 20.0±1.15 and 82.9±0.44%, germination durations were between 2.01±0.021 to 3.82±0.017 days, and germination indices were between 9.97±0.606 and 38.97±0.959. While the highest germination percentage (82.9±0.44%) was obtained from Dincer variety with 100 ppm TiO2NP pre-application, the lowest germination percentage (20.0±1.15% and 20.0±1.92%) was obtained from Balcı and Hasan Kendi varieties with 100 and 300 ppm TiO2NP pre-application. According to this result, although the highest germination percentage based on variety was obtained from the Dincer variety, the Balcı variety with the lowest germination percentage provided the most significant increase in the 200 ppm TiO2NPs application dose compared to the control. According to the germination percentage, it can be said that the most effective TiO2NPs application dose in Safflower varieties is 200 ppm. Further research on nanoparticles is needed to determine both the economical doses of TiO2NP pre-application and its uptake by the plant.
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18

Lieberman, Milton, and Diana Lieberman. "An experimental study of seed ingestion and germination in a plant-animal assemblage in Ghana." Journal of Tropical Ecology 2, no. 2 (1986): 113–26. http://dx.doi.org/10.1017/s0266467400000717.

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ABSTRACTFleshy fruits collected from abundant woody plants on the Accra Plains, Ghana, were fed to frugivorous species common in the area. Seeds of each plant species were retrieved from each frugivore species for germination trials, and ingested seeds compared to fresh seeds in germination percentage and rapidity. Altogether 85 plant X animal feeding combinations were attempted. In 73% of trials, ingestion did not affect germination percentage; the remain-ing trials were about equally divided between significant increases and decreases. Ingested seeds had significantly faster germination in 12%, and significantly slower germination in 8%, of the trials. Germination percentages in all samples of fresh seeds ( = 37%) did not differ signifi-cantly from those ingested seeds ( = 31%).There was a significant association between the effect of ingestion on both germination rapidity and percentage (significantly increased, decreased, or neither) and the plant species involved; there was no such association between the effect of ingestion and the animal species. Animals had inconsistent and frequently negligible effects on germination. Two-way analyses of variance (plants X animals) of germination percentage and of germination improvement showed that plant species were a significant source of variation and animal species were not. The fleshy-fruited plants of this assemblage are not generally dependent upon animals for successful germination of their seeds.
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Camargo, Haron Victor Ferreira, Letícia Siqueira Walter, Mônica Moreno Gabira, and Dagma Kratz. "Pre-germinative treatments and seed storage of Psidium cattleyanum Sabine morphotypes." COLLOQUIUM AGRARIAE 16, no. 4 (2020): 102–9. http://dx.doi.org/10.5747/ca.2020.v16.n4.a387.

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This study aimed to evaluate the germination behavior of two morphotypes of P. cattleyanumsubmitted to storage and pre-germinative treatments. The experiment was designed in a factorial scheme 2 x 3 x 3 (morphotypes x storage periods x pre-germinative treatments) completely randomized, using for each treatment 5 replicates with 30 seeds. The seeds were stored in semipermeable packages in a cold chamber at 5 ± 2 °C, for 14, 45,and 90 days. After each storage period, the seeds were submitted to treatments to overcome dormancy: immersion in water at 80 °C,kept in imbibition for 24 and 48 hand control, without any treatment. The seeds were arranged under moistened substrate in transparent plastic boxes and allocated in a germination chamber at 25 °C and photoperiod of 8 h for evaluation of germination percentage (G), germination speed index (GSI)and mean germination time (MGT). The red morphotype seeds showed higher germination percentages than the yellow morphotype and did not have a reduction in these values after 90 days of storage. MGT for yellow morphotype was lower in all pre-germinativetreatments. The GSI had better results at 45 days in the control treatments and 24 h in immersion in water and 90 days for the three pre-germinative treatments. The red morphotype does not require pre-germinative treatments, while the yellow morphotype presents better germination when the seeds are stored for 45 days and immersed in water at 80 °C for 48 h.
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20

Morgan, J. W. "Comparative Germination Responses of 28 Temperate Grassland Species." Australian Journal of Botany 46, no. 2 (1998): 209. http://dx.doi.org/10.1071/bt96117.

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The comparative germination biology of 28 perennial species native to the temperate grasslands of southern Victoria was tested using a single temperature regime (20/10˚C) in either constant darkness or 12 h diurnal white-light conditions. This temperature regime was chosen because it corresponds to the temperature found to produce substantial or optimal germination in other germination studies of grassland plants. Four germination attributes—time to the beginning of germination (germination lag), time taken to achieve 50% of final germination (germination speed –t50), percentage germination in diurnal light conditions and percentage germination in continuous darkness—were calculated for each species and correlated to three plant attributes: plant family, life form and seed weight. Germination lag was short for many species: 50% had a lag of less than 7 days, whilst 96% of species had begun germinating within 28 days. Germination speed was moderately fast for most species: 75% of species had a t50 of less than 28 days. Total percentage germination in diurnal light or continuous darkness was variable (0–98%), but many species (64%) germinated readily (> 50%) in at least one treatment. Twenty-four percent of species had germination substantially inhibited by darkness (i.e. Acaena echinata, Bulbine bulbosa, Eryngium ovinum, Podolepis sp. aff. jaceoides, Velleia paradoxa and Wahlenbergia luteola). No species had its germination promoted by darkness. Few correlations between plant and germination attributes were detected. Seed weight was not correlated with any of the three germination attributes, nor was life form or plant family correlated with germination speed or percentage germination in the light or dark. Germination lag, however, was significantly associated with plant family and, in a related way, with life form: species from the Liliaceae (geophytes) took longer to begin germination than species from the Asteraceae (hemicryptophytes). The ecological implications of the observed germination biology on the potential formation of persistent soil seed banks and seedling recruitment dynamics are discussed.
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Seadh, S. E., M. A. Badawi, M. A. Abdel-Moneamand, and M. M. E. Borham. "Germination and Seedling Parameters of Wheat as Affected by Storage Conditions." IOP Conference Series: Earth and Environmental Science 904, no. 1 (2021): 012035. http://dx.doi.org/10.1088/1755-1315/904/1/012035.

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Abstract A storage experiment was carried out from 27th April to 27thDecember, 2019 to study the effect of storage conditions i.e. methods (open air, under sunshade and in stores), packages type (normal, gunny, polyethylene and metal containers) and periods of seeds fumigation with phosphine (15, 30 and 45 days) on germination and seedling parameters of wheat. The experiment was arranged in a factorial experiment in randomized complete block design (R.C.B.D.) with four replications. Stored wheat seeds under sunshade produced the highest values of final germination percentage, germination index, mean germination time, plumule length, radical length, seedling vigor index and seedlings dry weight, followed by stored wheat seeds in stores. The highest germination index, mean germination time, plumule and radical lengths, seedling vigor index and seedlings dry weight were recorded in the samples of wheat seeds sealed stored in metal containers, followed by normal stored in gunny packages. The highest percentage of final germination was obtained by normal stored in gunny packages, followed by sealing storage in metal containers. The highest final germination percentage, germination index, mean germination time, plumule length, radical length, seedling vigor index and seedlings dry weight and the lowest abnormal seedlings percentage, rotten and sold seeds percentages, speed germination index, co-efficient of germination and germination energy percentage were recorded when treated with phosphine at the rate of 3 tablets/m3after 15 days from beginning the storage. This study recommended that stored wheat seeds under sunshade in metal packages and treating with phosphine at the rate of 3 tablets/m3 after 15 days from beginning the storage to enhance germination and seedlings parameter of wheat under the environmental conditions of Dakahlia Governorate, Egypt.
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22

Chitwood, Jessica, Ainong Shi, Michael Evans, et al. "Effect of Temperature on Seed Germination in Spinach (Spinacia oleracea)." HortScience 51, no. 12 (2016): 1475–78. http://dx.doi.org/10.21273/hortsci11414-16.

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Breeding heat-tolerant spinach is an important project to meet the demand of increasing spinach production in heat conditions. Seed germination is the early stage to test, screen, and develop heat-tolerant spinach genotypes. The objective of this research was to determine temperature effect on the seed germination percentage and to select heat-tolerant spinach genotypes. A total of nine spinach genotypes were used in this research. The germination experiment was conducted using seven temperatures: 10, 15, 20, 25, 30, 32, and 35 °C under growth chambers. The temperature trials were conducted using completely randomized design (CRD) with three replicates. Spinach seed germination percentage varied among the nine spinach genotypes under the seven temperatures, indicating that genetic variation for heat tolerance existed in the nine spinach genotypes. ‘Donkey’, ‘Marabu’, and ‘Raccoon’ showed higher seed germination percentage with over 70% at 30 and 32 °C, indicating the three spinach genotypes had heat tolerance for germination. However, all spinach genotypes except ‘Ozarka II’ dropped their germination percentages sharply to less than 30%; ‘Ozarka II’ had 63% germination under 35 °C, indicating it is a good source of heat tolerance for seed germination. The higher germination percentages above 30 °C of ‘Ozarka II’, ‘Donkey’, ‘Marabu’, and ‘Raccoon’ may indicate their potential as donors of heat-tolerant traits in spinach breeding program.
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Wade, Michael R., and D. F. Gilman. "Germination Response of Uniola latifolium." HortScience 25, no. 8 (1990): 915–16. http://dx.doi.org/10.21273/hortsci.25.8.915.

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Broadleaved uniola (Uniola latifolium Michx.) caryopses and florets were prechilled or not prechilled, supplied with potassium nitrate (KNO3) or water only, and diurnal germination temperature alternations of 15/30C or 20/30C were used. Caryopses and florets both reached 50% germination by 10 days after planting with KNO3; however, in water, caryopses reached 50% germination significantly earlier than florets (18 vs. 30 days). Germination temperature and prechilling had no effect on days to 50% germination. Final germination percentages were significantly higher for caryopses than florets at both germination temperatures and for caryopses germinated at 15130C than at 20/30C (85% vs. 74%). Significantly more seeds germinated with KNO3 than in water (85% vs. 57%). Prechilling had no effect on final germination percentage. Caryopses germinated with KNO3 at 15/30C achieved the highest final germination percentage.
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24

Kumar, Prabal, Kalpana Tyagi, Manish Singh, and Devendra Kumar. "Effect of Temperature, Media and Light on Germination of Prinsepia utilis Royle Seeds." Ecology, Environment and Conservation 28 (2022): 348–52. http://dx.doi.org/10.53550/eec.2022.v28i07s.057.

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Effect of temperature and media was studied on seed germination of Prinsepia utilis Royle (bhekal). Germination tests were conducted at four different temperatures viz. 5±1 o C, 15±1 o C, 25±1 o C and ambient room temperature with three germination media viz. top of germination paper (TP), between of paper (BP) and quartz sterilized sand at 90±5% relative humidity in a seed germinator for 21 days. Media and Temperature significantly affected seed traits. Seeds germinated well over wide range of temperature 15±1 o C, to ambient room. Significantly, higher germination percentage, germination value (GV) and minimum mean germination time (MGT) were obtained at 25±1 o C. lower than 25±1 o C and higher temperature (ambient room temperature) had adverse effect on germination of bhekal seeds. Among media, between paper (BP) was found the best in which maximum germination percentage, minimum MGT and maximum GV was recorded. Sand media was found inferior as compared to between and top of paper. Seed germination was unaffected with light.
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25

Finch-Savage, W. E., and C. I. McQuistan. "Performance of carrot seeds possessing different germination rates within a seed lot." Journal of Agricultural Science 110, no. 1 (1988): 93–99. http://dx.doi.org/10.1017/s0021859600079739.

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SummaryThe relationship between germination rate within a carrot seed lot and subsequent seedling performance was studied in four seed lots of different viability. Seedling performance was assessed using slope tests conducted under controlled conditions and in the field following fluid drilling under a range of environmental conditions. Germination rate within a seed lot was positively related to vigour measured by slope tests, percentage emergence and seedling weight, and was negatively related to the spread of emergence times (In variance days) and the coefficient of variation (c.v.) of seedling weights. Sowing seeds selected as fast germinating resulted in greater seedling emergence percentages over a range of environmental conditions than when sowing seeds selected as slowly germinating.
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26

Khajeh, M., S. A. Tabatabaei, O. Ansari, and F. Sharif Zadeh. "Improvement of Germination Characteristics and Enhancement of Antioxidant Enzymes Activity of Safflower (Carthamus tinctorius L.) Aged Seeds by Used of Gibberellin." Cercetari Agronomice in Moldova 48, no. 3 (2015): 33–41. http://dx.doi.org/10.1515/cerce-2015-0039.

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AbstractSeed priming is one of the methods that can be taken to counteract the adverse effects of abiotic stress, also Seed priming treatments have been used to reduce the damage of aging and invigorate their performance in many crops. Objective of this study was to evaluation the effect of gibberellin on germination characteristics and antioxidant enzymes of safflower seeds after aging. Experimental design was a factorial one with complete randomized design with three replications. The first factor was priming by gibberellin (0 and 50 ppm) and non-primed seeds (control) and the secondary factor was combinations of four levels of aging (0, 1, 3 and 5 days of aging, at 43°C). The results showed that the priming and aging effects on germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were significant, but on mean time to germination not significant. Results showed that, the highest germination characteristics such as; germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were attained from priming by gibberellins, under non aged conditions. Also, our results indicated that seed aging is related to decrease of enzymes activity and may contribute to low seed germination efficiency, also priming increases enzyme activity and increases enzyme activity with priming treatment may contribute to improve germination characteristics. The general decreases in enzyme activity in the seed lowers the respiratory capacity, which in turn lowers both the energy (ATP) and assimilates supply of the germinating seed.
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27

Barney, Danny L. "Germination Characteristics of Thin-leaved Huckleberry (Vaccinium membranaceum)." HortScience 32, no. 3 (1997): 538B—538. http://dx.doi.org/10.21273/hortsci.32.3.538b.

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Seeds of V. membranaceum germinated in petri dishes fresh (undried), airdried for 7 days, or cold-stored for 1 or 6 years exhibited similar germination vs. time curves. Dry storage at 0–4°C for 1 or 6 years did not reduce the percentage of germination compared to fresh seeds. Cold stratification at 0–4°C slowed germination by extending the initial lag phase compared to unstratified seed. Stratification for 28 to 56 days delayed germination by ≈2 weeks. This pattern held true for fresh (undried) seed, seed air-dried for 7 days, and seed cold-stored for 6 years. Surface sterilization for 20 or 30 minutes with a 0.5% aqueous solution of sodium hypochlorite reduced fungal and bacterial contamination of germinating seeds without adversely impacting germination. Treatment of V. membranaceum seeds with captan or mancozeb fungicide inhibited germination by extending the lag phase and reducing the germination vs. time slope of the exponential phase. Mancozeb-treated seeds exhibited a lower percentage of germination than did controls, and often developed necrotic radical tips.
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28

Bratcher, Carlma B., John M. Dole, and Janet C. Cole. "Stratification Improves Seed Germination of Five Native Wildflower Species." HortScience 28, no. 9 (1993): 899–901. http://dx.doi.org/10.21273/hortsci.28.9.899.

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The germination responses of wild blue indigo [Baptisia australis (L.) R. Br.], purple coneflower [Echinacea purpurea (L.) Moench.], Maximilian sunflower (Helianthus maximiliani Schrad.), spike goldenrod (Solidago petiolaris Ait.), and Missouri ironweed (Vernonia missurica Raf.) seeds after 0, 2, 4, 6, 8, or 10 weeks of stratification at 5C were investigated. Seed viability was determined using triphenyl tetrazolium chloride staining and germination based on the percentage of viable seeds. Germination percentage (GP) increased in all five species as weeks of stratification increased. Days to first germination and germination range (days from first to last germinating seed) decreased with increasing weeks of stratification, but the effect beyond 4 to 6 weeks was minimal. The number of weeks of stratification for maximum GP was 4 for purple coneflower, 6 for Maximilian sunflower, 8 for Missouri ironweed, and 10 for wild blue indigo and spike goldenrod.
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29

Rahul, Ranjan, and Krishna Kumar Jai. "STUDY ON GERMINATION OF RAJMA (PHASEOLUS VULGARIS.L) IN AMENDED SOIL." International Journal of Education &Applied Sciences Research 3, no. 7 (2016): 37–42. https://doi.org/10.5281/zenodo.10700217.

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<strong>Abstract </strong> The purpose of this research was to investigate the effects of applying Plain Soil, Plain soil and vermicompost, Plain soil and Neem cake, Plain soil and inorganic fertilizers on germination of&nbsp; Rajma. Significant differences in germination percentage were observed between amended soil and Control (plain Soil). The germination percentage was found maximum in plain soil followed by amended soil with urea followed by amended soil with vermicompost on 3<sup>rd</sup> day of seeding. On 4<sup>th</sup> day of seeding it was 100% in plain soil, 60% in vermicompost amended soil 80% in urea amended soil and 50% in neem amended soil. The conductivity and the protein content of the root stem and leaf has also been estimated. <strong>Keywords:</strong> Germination percentage, vermicompost, plain soil
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30

Matthews, S. "Approaches to the indirect evaluation of germination and vigour." Scientia Agricola 55, spe (1998): 62–66. http://dx.doi.org/10.1590/s0103-90161998000500011.

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In comparisons of six seed lots of different F1 hybrid cultivars of cauliflower with similarly high laboratory germinations (above 90%) separation in germination was achieved after controlled deterioration (C.D.) at 24% moisture content (m.c.) and 45 0C for 24 hours. This measure of vigour was related to the position of the lots on the seed survival curve and was highly predictive of the longevity of the lots when stored at 15% m.c. and 20 0C for 12 and 16 weeks. When each seed lot was deteriorated at 24% m.c. for increasing times (from 0 to 36 hours) a reduction in the subsequent percentage germination was seen, which, using probit transformed percentages, was significantly and linearly related to the leakage of electrolytes into seed soak water over 24 hours. The case is made for an approach to the indirect evaluation of germination and vigour using C.D. followed by measurements of leakage that could be more discerning and rapid than the present laboratory germination test.
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31

Lone, A. B., R. C. Colombo, B. L. G. Andrade, L. S. A. Takahashi, and R. T. Faria. "Physical characterization of Rhipsalis (Cactaceae) fruits and seeds germination in different temperatures and light regimes." Brazilian Journal of Biology 76, no. 2 (2016): 367–73. http://dx.doi.org/10.1590/1519-6984.15914.

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Abstract The germination characteristics of the native cactus species are poorly known, being the temperature and the light the factors that the most interferes in that process. Thus, the objective of the present work was to characterize the fruits and evaluate the influence of the temperature and the light in the seed germination of Rhipsalis floccosa, Rhipsalis pilocarpa and Rhipsalis teres. The tested constant temperatures were 15, 20, 25, 30 and 35 °C and the alternate of 20-30 °C and 25-35 °C in a photoperiod of 10 hours, and with determination of the most appropriate temperature, the germination was tested in light absence. The germination percentage, the index of germination speed and medium time of germination were evaluated. For R. floccosa, the highest germination percentage was at 20 °C. For R. pilocarpa and R. teres, the highest germination percentages occurred in 15 °C and 20 °C. There was correlation to germination percentage between the three species, indicating that they had similar germination behavior. Total absence of germination was verified for the three species in condition of light absence. In conclusion, the temperature of 20 °C is the most suitable for the seed germination of R. floccosa. For the species R. pilocarpa and R. teres, the temperatures of 15 and 20 °C are the most suitable.
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32

Bush, Edward, Paul Wilson, and Gloria McClure. "335 Light Exposure and Gibberelic Acid Effects on Common Carpetgrass (Axonopus affinis Chase) and Centipedegrass [Eremochloa ophiuroides (Munro) Hack.] Seed Germination." HortScience 35, no. 3 (2000): 449E—449. http://dx.doi.org/10.21273/hortsci.35.3.449e.

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A study to determine the influence of light duration on seed germination was performed in a temperature-controlled growth chamber. Light treatments consisted of 0 (control), 6, 8, 10, 12 and 14 h of light exposure. Cool fluorescent light bulbs provided 19 μMol·m-2·s-1 light. Fifty seeds of each treatment were placed into separately labeled 6.0-cm-diameter petri dishes lined with Whatman #42 filter papers moistened with 2 mL of distilled water. Seed of both species germinated poorly in the control treatment. Mean time of germination (MTG) and germination percentage increased for both species when seeds were exposed to light. Pre-soaking seed in gibberellic acid (GA) significantly improved germination percentages of both species compared to the untreated control. Centipedegrass germination percentage and MTG also increased with light exposure. Carpetgrass seed germination was not enhanced by GA treatments with light exposure. The results of this experiment suggests that, if seed are covered too deeply, excluding light, MTG and percentage germination will be reduced. However, pre-soaking seed in a GA solution can improve dark germination by as much as 50% for both grass species.
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33

Eren, Erkan, Sıtkı Ermis, Guleda Oktem, and Ibrahim Demir. "Seed Longevity Potential Predicted by Radicle Emergence (RE) Vigor Test in Watermelon Seed Cultivars." Horticulturae 9, no. 2 (2023): 280. http://dx.doi.org/10.3390/horticulturae9020280.

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The study was conducted to test whether radicle emergence (RE) would correlate with the storage potential of ten seed lots of watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) hybrid cultivars (&gt;98% normal germination). The RE test was performed by frequent counting radicle emergence (2 mm) percentage between 34 h and 60 h after germination was set up at 25 °C in the dark. Seed longevity was hermetically determined by artificial storage of seeds (air and waterproof) at 17 ± 0.3% of seed moisture and at 35 ± 2 °C over 63 days. Twelve seed samples were taken out during aging, and seed survival curves were constructed based on normal germination percentages conducted at 25 °C for seven days in the dark. The seed longevity criterion was P50 (time for the germination to fall to 50%), which was determined through probit analysis by using survival curves. Correlation analysis showed that RE counts at 42 h during germination were highly correlated (p &lt; 0.01) with initial seed quality, Ki (r = 0.7538), and the half-viability period, P50 (r = 0.7936). Pre-storage normal germination percentages of seed lots were not related to longevity. Results showed that the RE vigor test has the potential to predict longevity in highly germinating hybrid watermelon seed lots.
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34

Soltani, Elias, Farshid Ghaderi-Far, Carol C. Baskin, and Jerry M. Baskin. "Problems with using mean germination time to calculate rate of seed germination." Australian Journal of Botany 63, no. 8 (2015): 631. http://dx.doi.org/10.1071/bt15133.

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Seed scientists and other plant biologists are interested in the measurement of germination because seeds from different individuals, populations, seed lots and treatments can differ in germination percentages, rate (speed) and uniformity. Mean time to germination (MGT) is a measure of the rate and time-spread of germination; however, there is a problem with using this method to calculate germination rate. MGT does not show the time from the start of imbibition to a specific germination percentage. MGT has been used to compare specific pairs or groups of means and to evaluate seed vigour. However, it is not the real time to mean germination but just an index of germination speed. Using MGT is not correct for ANOVA, post-ANOVA or the other comparison tests, because it does not show time to a specific germination percentage. Thus, we recommend that t50 be used instead of MGT. The t50 has all benefits of MGT, but it does not have the problems of MGT in treatment comparisons.
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35

Fay, Amy M., Mark A. Bennett, and Steven M. Still. "Osmotic Seed Priming of Rudbeckia fulgida Improves Germination and Expands Germination Range." HortScience 29, no. 8 (1994): 868–70. http://dx.doi.org/10.21273/hortsci.29.8.868.

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Low-vigor seeds of black-eyed Susan (Rudbeckia fulgida Ait.) primed in aerated -1.3 MPa KNO3 for 7 days at 30C in darkness had double the total germination percentage at 30C and one-half the mean time of germination as nonprimed seeds. Priming the seeds in polyethylene glycol rather than KNO3 generally resulted in lower total germination percentage and longer mean time of germination. Osmotic priming increased total germination percentage and germination rate of seeds germinated at 21.9 to 32.2C, but the priming benefit on total germination percentage was greater at ≤27.6C. Total germination percentage of primed and nonprimed seeds was highest at 28.8 to 32.2C.
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36

Qin, Yingbo, Boyang Geng, Li-E. Yang, and Deli Peng. "Non-deep physiological dormancy and germination characteristics of Primula florindae (Primulaceae), a rare alpine plant in the Hengduan Mountains of southwest China." PeerJ 11 (April 28, 2023): e15234. http://dx.doi.org/10.7717/peerj.15234.

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Timing of seed germination is directly related to the survival probability of seedlings. For alpine plants, autumn-dispersal seeds should not germinate immediately because the cold temperature is not conducive to the survival of seedlings. Seed dormancy is a characteristic of the seed that prevents it from germinating after dispersal. Primula florindae is an alpine perennial forb endemic to eastern Tibet, SW China. We hypothesized that primary dormancy and environmental factors prevent seeds of P. florindae to germinate in autumn and allow them to germinate at the first opportunity in spring. We determined how GA3, light, temperature, dry after-ripening (DAR) and cold-wet stratification (CS) treatments affect seed germination by conducting a series of laboratory experiments. Firstly, the effects of gibberellic acid (GA3; 0, 20, and 200 mg L−1) on germination of freshly shed seeds at alternating temperatures (15/5 and 25/15 °C) were immediately investigated to characterize seed with a physiological dormancy component. Then, the fresh seeds treated with 0, 3, and 6 months of after-ripening (DAR) and cold-wet stratification (CS) were incubated at seven constant (1, 5, 10, 15, 20, 25, and 30 °C) and two alternating temperatures (5/1, 15/5, and 25/15 °C) at light and dark conditions. Fresh seeds were dormant, which only germinated well (&gt;60%) at 20, 25, and 25/15 °C in light but not at ≤15 °C and to higher percentages in light than in dark. GA3 increased germination percentage of fresh seeds, and DAR or CS treatments increased final germination percentage, germination rate (speed), and widened the temperature range for germination from high to low. Moreover, CS treatments reduced the light requirement for germination. Thus, after dormancy release, seeds germinated over a wide range of constant and alternating temperatures, regardless of light conditions. Our results demonstrated that P. florindae seeds have type 2 non-deep physiological dormancy. Timing of germination should be restricted to early spring, ensuring a sufficient length of the growing season for seedling recruitment. These dormancy/germination characteristics prevent seeds from germinating in autumn when temperatures are low but allow them to germinate after snowmelt in spring.
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37

Azimi, Reyhaneh, Mohammad Kia Kianian, and Mohammad Pessarakli. "INVESTIGATION OF THE TiO2 NANOPARTICLES EFFECT ON SEED GERMINATION CHARACTERISTICS OF ZIZIPHORA CLINOPODIOIDES LAM." Plant Breeding and Seed Science, no. 79 (December 31, 2019): 49–61. http://dx.doi.org/10.37317/pbss-2019-0004.

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Improvement in the rate and amount of germination of seeds has a very important effect on the establish-ment of primary seedlings and the increase of rangeland production. The rapid and uniform germination of seeds leads to the successful establishment of plants. The use of nanoscale materials can help germinate faster seeds. Therefore, in this study, the effects of TiO2 nanoparticles in concentrations of 0, 10, 20, 30, 40, 60 and 80 mg / l on the rate and speed of seed germination of Ziziphora clinopodioides Lam. paid. This design was carried out in a completely randomized design with four replications for 20 days at a constant temperature of 20°C under 12 hours of light and 12 hours of darkness at the Germinator of Natural Resources Faculty of the Ferdowsi University of Mashhad. The results showed that germination percentage of treated seeds with TiO2 nanoparticles increased to 23% ppm compared to control treatment. Also, in other concentrations of other nanoparticles, there was a positive effect on speed and germination percentage, so that the effect of different concentrations of nanoparticles on germination characteristics of Ziziphora clinopodioides Lam. seeds was significant. The highest germination percentage was observed in the concentration of 30 ppm and the lowest germination rate at 30 and 20 ppm concentrations. In high concentrations of TiO2 nanoparticles, no positive effects were observed on the germination characteristics of seed Ziziphora clinopodioides Lam. To conclude the use of TiO2 nanoparticles can be improved by improving the seed germination properties of the medicinal plant Ziziphora clinopodioides Lam. that cause increases plant’s establishment in natural areas.
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38

ADELANI, D. O., R. A. SULEIMAN, and U. U. EMEGHARA. "ASSESSMENT OF PERIODS OF SAND PRIMING AND HYDRO-PRIMING ON THE GERMINATION OF AFRICAN EBONY (Diospyros mespiliformis Hochst)." Journal of Agricultural Science and Environment 17, no. 1 (2017): 83–90. http://dx.doi.org/10.51406/jagse.v17i1.1792.

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Diospyros mespiliformis (Hochst) is a multipurpose, agro-forestry tree species with diverse environmental and ecological significances. However, low percentage germination associated with its seeds has limited its domestication. To overcome this challenge, there is need to adopt cheap, fast, safe, natural and adoptable physiological techniques such as sand priming and hydro-priming. There is paucity of quantified information on natural and safe methods of relieving dormancy as sand priming and hydro-priming. Most of methods of breaking dormancy as use of acid are not simple, safe and adoptable by farmers who practices agro-forestry. In the light of this, these experiments were conducted to assess the periods of sand priming (0, 2, 4, 6 and 8 weeks) and hydro-priming (0, 2, 4, 6 and 8 weeks) on the germination of D. mespiliformis. Both experiments were laid down in completely randomized design with four replicates. Mean germination times were calculated, and result revealed that a significant increase in percentage germination was recorded with increasing periods of exposing seeds to sand priming up to four weeks. The percentage germination ranged between 28%-93.25% for control (0) and four weeks sand priming respectively. Least value of 8.92 day was recorded for mean germination time of control (0 sand priming). A significant increase in percentage germination was recorded with increasing hours of hydro-priming up to 24 hours. The percentage germination was ranged between 25% - 100% for control (0) hour to 24 hours. Highest germination percentages were recorded in seeds subjected to sand priming for 4weeks (93.25%) and those hydro-primed for 24hours (100%). These results are recommended for mass production of D. mespiliformis in agro-forestry nurseries.&#x0D;
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39

Pavlov, A. V., V. G. Verzhuk, and D. D. Bondaruk. "THE EFFECT OF PHYTOHORMONES AND LIGHT ON THE GERMINATION OF APPLE POLLEN WITH REDUCED VIABILITY." Proceedings on applied botany, genetics and breeding 180, no. 4 (2020): 27–31. http://dx.doi.org/10.30901/2227-8834-2019-4-27-31.

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Background. Studying the effect of phytohormones and light on the germination of apple pollen with reduced viability on an artificial nutrient medium is of importance, because it may facilitate an increase in the germination percentage among plant samples promising for breeding after their long-term preservation under low temperatures.Materials and methods. Pollen viability of the apple-tree cultivar ‘Krasnolistnaya’ was measured by germinating it on an artificial nutrient medium containing 10% sucrose solution and 0.8% agar. Drops of a distilled water suspension of pollen with added phytohormones were applied to the surface of the nutrient medium. Pollen was germinated in the dark (24 hours in a thermostat at 21°C) and under light (in an artificial light chamber at 21°C with a photoperiod of 16 hours of light / 8 hours of darkness). Results. Pollen of cv. ‘Krasnolistnaya’ with reduced viability most effectively germinated with the use of gibberellin at a concentration of 1 mg/l and 10 mg/l: the germination percentage was 22.3±0.8% and 21.8±1.3% respectively (сf. 10.9±1.5% in the reference). The most effective combination of phytohormones was gibberellin 10 mg/l + kinetin 10 mg/l – the percentage of germination was 22.8±6.3%; kinetin 1 mg/l + indolylbutyric acid 1 mg/l – the percentage of germination was 17.5±5.9% vs. 10.9±1.5% in the reference.Conclusion. Phytohormones were observed to have a significant effect on the germination of apple pollen with reduced viability. Effective concentrations and combinations of phytohormones that promote the germination of pollen with reduced viability have been identified. Light does not affect the germination of pollen with reduced viability.
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40

Reis, Renata Conduru Ribeiro, Bárbara França Dantas, and Claudinéia Regina Pelacani. "Mobilization of reserves and germination of seeds of Erythrina velutina Willd. (Leguminosae - Papilionoideae) under different osmotic potentials." Revista Brasileira de Sementes 34, no. 4 (2012): 580–88. http://dx.doi.org/10.1590/s0101-31222012000400008.

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Some environmental factors, including water availability, may influence seed germination. This study investigated the germination of E. velutina seeds submitted to different osmotic potentials and mobilization of reserves during water-stress. Scarified seeds were arranged in paper rolls and soaked in solutions of Polyethylene Glycol (PEG) prepared in osmotic potentials 0.0, -0.2, -0.4, -0.6, and -0.8 MPa and kept into a seed germinator, at 25 °C, and 12/12 h photoperiod (L/D), during 10 days. The percentage, mean time, mean speed, germination speed index; as well as the germination uniformity coefficient were assessed. During germination process the total soluble sugars, reducing sugars, soluble protein, and total amino acids were quantified in the cotyledon, hypocotyl and radicle of soaked seeds and cotyledons of quiescent seeds (control). There was influence of osmotic potential on E. velutina seed germination. The germination percentage remained at high levels until -0.6 MPa and above this osmotic potential there has been no germination. The mobilization of stored reserves of carbon and nitrogen in E. velutina seeds was also influenced by water-stress. There was sensitiveness between -0.2 and -0.6 MPa; however, the degradation and the mobilization of reserves was slower when the osmotic potential decreased.
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41

El Mardi, M. O., E. Consolacion, O. AI-Manthari, and H. AI-Nabhani. "Factors Influencing Date Pollen Viability." Journal of Agricultural and Marine Sciences [JAMS] 2 (January 1, 1997): 55. http://dx.doi.org/10.24200/jams.vol2iss0pp55-58.

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Pollen of seven date cultivars was stored at 4.5 -5.0'C using desiccator with or without silica gel and in vials outside the desicator as a control. Germination percentage was determined microscopically with a hemacytometer after harvest and at three and six months of Storage, respectively, using a boric acid-sucrosce medium. Cultivar genotype did out influence the viability of pollen. Pollen stored for six months gave a higher germination percentage (45.9%) than three months (40.9%) indicating that pollen requires more than three months at 4.5 - 5C for the reactivation or its growth factors. An increase in relative humidity in storage containers resulted in reduced germination profiles. Germination percentages were 62.2, 42.9 and 22.79% at 18. 55 and over 55 relative humidity, respectively. Pollen stored in desiccator with and without silica gel gave a higher germination profile after six months than at three months storage whereas the control gave the lowest. The highest germination percentage was observed in pollen kept in a desiccator in the presence of silica gel for six months.
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42

Mensah, S. I., P. O. Ejeagba, and K. Okonwu. "Effects of GA3, BAP and KNO3 on the Germination and DNA Content of Cucumber (Cucumis sativus L.)." Greener Journal of Agricultural Sciences 10, no. 2 (2020): 57–62. https://doi.org/10.15580/gjas.2020.2.021720037.

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Effects of gibberellic acid (GA3), 6-benzylaminopurine (BAP) and potassium nitrate (KNO3) on the seed germination and DNA concentration of cucumber (Cucumis sativus) radicle were assessed. The concentrations of these growth stimulants were 0 mM, 1 mM, 5 mM and 10 mM. The cucumber seeds were surface sterilized in ethanol for 5 minutes and rinsed with distilled water before pretreatment with these growth stimulants. The germination study was allowed to stand for 14 days and DNA concentration of cucumber radicle with the highest germination count was determined for each growth stimulant. The study showed that cucumber seeds had higher germination count under the light condition than in the dark condition. However, it is not statistically different. The study also showed that percentage germination of cucumber seeds was enhanced by GA3 (57 – 72%) and BAP (62 – 70%) when compared to the Control (50%) except KNO3 (41 – 44%). Across the treatments, GA3 gave the highest germination percentage followed by BAP with 5 mM concentration producing the highest germination count while 10 mM recorded the highest in KNO3. The DNA concentration of the cucumber radicle that produced these highest germination percentage are: GA3 (47.40 ng/µl), BAP (98.87 ng/µl), KNO3 (103.23 ng/µl) and Control (79.73 ng/µl). The analysis of variance (ANOVA) showed that treatments are significant at p-value (0.0001) &lt; 5% significant level for cucumber seed. The study recommends the use of 5 mM GA3 in germinating cucumber seeds.
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43

Qaderi, Mirwais M., and Paul B. Cavers. "Interpopulation variation in germination responses of Scotch thistle, Onopordum acanthium L., to various concentrations of GA3, KNO3, and NaHCO3." Canadian Journal of Botany 78, no. 9 (2000): 1156–63. http://dx.doi.org/10.1139/b00-088.

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The effects of GA3, KNO3, and NaHCO3 on germination percentages and rates were determined for cypselas (seeds) of four local populations of Scotch thistle, Onopordum acanthium L., collected during a 2-year period. Three (1996, stored cypselas) and four (1997, fresh cypselas) concentrations (0.025, 0.05, and 0.1% for both years, plus 0.0125% for 1997) of the three compounds were used. Both freshly collected cypselas and those stored for 67 days at room temperature exhibited a range of germination responses from readily germinable to strongly dormant. GA3, even at a very low concentration, caused most viable cypselas to germinate, but 5-6% of viable cypselas in two populations did not respond to any GA3 treatment. In general, dry-stored cypselas germinated faster than fresh ones. Populations with more germinability (= proportion of cypselas capable of germinating) responded faster to GA3 than those with less germinability. KNO3 usually increased germination percentages but reduced the rate of germination. When treated with KNO3, stored cypselas from the two populations with less germinability germinated more slowly than those of the other two. In general, germination percentage decreased with an increase in NaHCO3 concentration. Overall, stored cypselas that were treated with NaHCO3 germinated more slowly than those in the control. The large differences among four local populations in response to these chemicals suggest that the effects of germination stimulators and other chemicals should be observed on several populations per species.Key words: cypsela, germination promoters, germination inhibitors, local populations, Onopordum acanthium, Scotch thistle.
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44

Nayak, D., L. Behera, V. Prajapati, and D. Jadeja. "Genetic variability in seed and seedling traits of Jatropha curcas L." Journal of Non-Timber Forest Products 19, no. 2 (2012): 111–16. http://dx.doi.org/10.54207/bsmps2000-2012-m027g5.

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Twenty two Jatropha curcas L., seed sources representing the states of Gujarat, Maharashtra, Madhya Pradesh, Uttarakhand, Haryana, Punjab and Rajasthan were evaluated to assess the genetic variability of seed and seedling traits during April to October, 2005 under statistically randomized block design with three replications to study the genetic variability of seed and seedling traits of Jatropha curcas L. The characters studied were 100 seed weight, seed length, seed width, germination percentage, germinalive energy, seedling height, seedling collar diameter, number of leaves and seedling survival percentage. Genotypic variances were higher than the environmental variances for all the characters, thereby, indicating their importance for selection. The genotypic and phenotypic coefficient of variation was high for seedling survival percentage, seedling height and germination percentage. High heritability coupled with high genetic advance expressed as percent mean was observed for seedling survival. germinative energy, seedling height and germination percentage. The characters namely seedling collar diameter, 100 seed weight and seed width exhibited high heritability coupled with medium genetic advance expressed as percent mean.
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45

Guariz, Hugo Roldi, Gabriel Danilo Shimizu, Jean Carlo Baudraz de Paula, Huezer Viganô Sperandio, and Rodrigo Yudi Palhaci Marubayashi. "Germinative potential of ‘Pata-de-Vaca’ seeds at different maturation stages under various temperatures." Ornamental Horticulture 28, no. 3 (2022): 367–75. http://dx.doi.org/10.1590/2447-536x.v28i3.2505.

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Abstract Bauhinia monandra Kurz has ornamental characteristics as the shape of its crown and the exuberance of its flowering, being and is widely cultivated in urban afforestation in Brazilian regions. The production of forest seedlings is essential not only to meet the demand of urban afforestation, but also to conserve forests. However, studies on B. monandra regarding the degree of fruit maturity on seed performance are scarce. Thus, the objective of the current work was to verify the germination potential of B. monandra seeds at different stages of maturation at five constant temperatures. The germination percentage, germination speed index (GSI), mean germination time (t), relative germination frequency, and mean germination speed (MGS) were evaluated. Initially, the mature seeds had a water content of 8.5% and the immature seeds of 68.3%. Mature seeds reached high germination percentages, above 96% for temperatures of 25, 30, and 35 ºC. The immature seeds demonstrated low germination percentages, reaching the highest percentage of 33% for the temperature of 25 ºC, followed by the temperatures of 30 ºC and 35 ºC, with respective percentages of 32% and 20%. Mature seeds demonstrated the highest GSI value (11.79) for the estimated temperature of 26.94 ºC and immature seeds the highest value of t (7.85) for the temperature of 26.56 ºC. It is concluded that mature seeds aggregate higher germination at an estimated temperature of 24.72 ºC and that immature seeds present unsatisfactory germination performance.
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46

RIIS, PREBEN, ELLA MEILING, and JØRGEN PEETZ. "DETERMINATION OF GERMINATION PERCENTAGE AND GERMINATION INDEX-COLLABORATIVE TRIAL AND RUGGEDNESS TESTING." Journal of the Institute of Brewing 101, no. 3 (1995): 171–73. http://dx.doi.org/10.1002/j.2050-0416.1995.tb00857.x.

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47

Beyhan, N., and U. Serdar. "Assessment of pollen viability and germinability in some European chestnut genotypes (Castanea sativa L.)." Horticultural Science 35, No. 4 (2008): 171–78. http://dx.doi.org/10.17221/23/2008-hortsci.

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Pollen viability and germinability in some European chestnut genotypes was assessed in this study. In 10 chestnut genotypes, percentages of pollen viability were generally high and often around or over 80%. The pollen germination percentages of the genotypes were significantly affected by media sucrose concentrations. At optimum sucrose concentrations pollen germination percentages varied between 21.97 and 43.68% in 2004, 3.95 and 31.97% in 2005 and 6.79 and 31.03% in 2006, across all genotypes. The highest pollen germination percentage was obtained from 10% sucrose concentration in all years. Although, in 2006, a highly marked positive correlation (r = 0.80) was determined for the viability and germination percentages, no significant relation between the viability and germination percentages r = &amp;ndash;0.54 and r = &amp;ndash;0.05, respectively) was found in 2004 and 2005. In 2005 and 2006, germination percentages declined compared to 2004.
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48

Kheloufi, Abdenour, Lahouaria Mounia Mansouri, and Faiza Zineb Boukhatem. "Application and use of sulphuric acid pretreatment to improve seed germination of three acacia species." REFORESTA, no. 3 (July 1, 2017): 1. http://dx.doi.org/10.21750/refor.3.01.25.

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The experiments were led to study the effect of pretreatments and their duration on germination behavior of three Acacia species A. cyanophylla Lindl., A. farnesiana L. and A. decurrens Willd. by analyzing three parameters (GP: germination percentage; MGT: germination mean time (days) and GRI: germination rate index) for various times of incubation (5, 10 and 15 days) in Petri dishes. Pre-sowing treatment included immersion in concentrated sulphuric acid for 60, 90 and 120 minutes. The sowing in distilled water (Control) had no positive effect on the germination induction. Generally, the seed pretreatments were very useful to improve germination. Time of immersion significantly (P &lt; 0.0001) affected GP, MGT and GRI in all species. Increasing the duration of sulphuric acid immersion (from 60 to 120 min) improved the germination percentages for A. cyanophylla and A. farnesiana seeds to (98% and 99%), respectively. However, increasing this duration had a negative effect on A. decurrens seed germination, reducing the final germination percentage from 97% at 60 minutes of immersion to 43% at 120 minutes.
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49

Chimsa, Dereje, and Tesfaye Ashine. "Germination Response of Juniperus procera Seed to Temperature and Pre-sowing Treatments." East African Journal of Forestry and Agroforestry 7, no. 1 (2024): 471–83. https://doi.org/10.37284/eajfa.7.1.2563.

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Juniperus procera is an indigenous tree species of Ethiopia that has high economic and ecological importance. Propagation of seedlings had poor germination due to dormancy. Enhancing the germination of Indigenous trees particularly, Juniperus procera seeds is the challenge encountered by nurseries. This study was conducted in the laboratory of the Central Ethiopia Forestry Development Center, with the aim to investigate the effect of seed pre-pretreatments (soaking in cold water for 24 hours, scarification, soaking in hot water for10 minutes, and no seed pre-treatment) and temperature regimes (20℃, 30℃, 40℃, and room temperature) on germination. Twenty-five quality seeds per treatment were sown on a petri dish incubated under temperature regimes using a completely randomized design with four replicates. Germination starting and closing dates, germination period, percentages, mean germination time and index were calculated and analysis of variance was done. The result of ANOVA revealed a significant (P&lt;0.05) effect of pre-treatments and temperature regimes on seed germination closing dates, period, percentage and index over the control. There was a non-significant effect of temperature on germination starting and mean germination time. There was also a non-significant combined effect of pretreatments and temperature on the germination period. Among pre-treatments, scarification achieved the best germination and from temperature regimes, 20℃ attained the best result over the other treatments. The highest germination percentage (74%) was obtained under 20℃ with scarification. The lowest germination percentage (18 %) was recorded under the control at 30℃. No germination was observed under 40℃. The shortest germination starting, closing dates and period were obtained under 20℃ with scarification. The lowest mean germination time and the greatest germination index were recorded under 20℃ with scarification. Compared to the control, almost all pre-treatments and temperature regimes increased germination with decreasing temperature regimes. Hence, in raising Juniperus seeds, it is recommended to use scarification under 20℃.
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50

Carrasco Cruzado, Angela Yoset, NatalyAndrea Villegas Herrera, Segundo Eloy López Medina, et al. "Influencia del ácido giberélico en la germinación de Physalis peruviana“aguaymanto”." Manglar 22, no. 1 (2025): 61–66. https://doi.org/10.57188/manglar.2025.007.

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Physalis peruvianaL., known as “aguaymanto”, has gained economic relevance in recent years due to its high nutritional value. However, there is little information on its germination studies. This research focused on determining the influence of gibberellic acid on the germination of P. peruviana“aguaymanto”. During the experimental phase, P. peruviana L. “aguaymanto” seeds treated with different concentrations of gibberellic acid were used (T1: Control, T2: 100 ppm, T3: 250 ppm and T4: 500 ppm). 90 seeds were selected and sown in germinators to analyze their emergence. The results showed that the concentration of 500 ppm (T4) reached the highest average germination percentage, with 74%. Furthermore, the one-way ANOVA test revealed statistically significant differences in germination percentage, and Tukey's Post hoc test confirmed that T4 (500 ppm) increased this percentage. The average emergence percentage was 83.33% and the coefficient of variation was 7.98%, indicating a high homogeneity in the emergence percentages of P. peruviana“aguaymanto”. It is concluded that the concentration of 500 ppm of gibberellic acid optimizes the germination and emergence of P. peruviana “aguaymanto”, and its use is recommended in propagation research.
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