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Journal articles on the topic 'Ghost proteins'

Author: Grafiati

Published: 15 June 2024

Last updated: 31 July 2025

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1

Ali, Randa H., Mohamed E. Ali, and Reham Samir. "Production and Characterization of Bacterial Ghost Vaccine against Neisseria meningitidis." Vaccines 11, no. 1 (2022): 37. http://dx.doi.org/10.3390/vaccines11010037.

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Bacterial ghosts (BGS) are empty non-living envelopes produced either genetically or chemically. This study investigated a novel chemical protocol for the production of Neisseria meningitidis ghost vaccine using tween 80 followed by a pH reduction with lactic acid. For our vaccine candidate, both safety and immunogenicity aspects were evaluated. The ghost pellets showed no sign of growth upon cultivation. BGS were visualized by scanning electron microscopy, illustrating the formation of trans-membrane tunnels with maintained cell morphology. Gel electrophoresis showed no distinctive bands of t

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Golan, D. E., C. S. Brown, C. M. Cianci, S. T. Furlong, and J. P. Caulfield. "Schistosomula of Schistosoma mansoni use lysophosphatidylcholine to lyse adherent human red blood cells and immobilize red cell membrane components." Journal of Cell Biology 103, no. 3 (1986): 819–28. http://dx.doi.org/10.1083/jcb.103.3.819.

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Human red blood cells (RBCs) adhere to and are lysed by schistosomula of Schistosoma mansoni. We have investigated the mechanism of RBC lysis by comparing the dynamic properties of transmembrane protein and lipid probes in adherent ghost membranes with those in control RBCs and in RBCs treated with various membrane perturbants. Fluorescence photobleaching recovery was used to measure the lateral mobility of two integral membrane proteins, glycophorin and band 3, and two lipid analogues, fluorescein phosphatidylethanolamine (Fl-PE) and carbocyanine dyes, in RBCs and ghosts adherent to schistoso

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Jawale, Chetan V., Nithiphonh Somsanith, Seong Kug Eo, Sang-Youel Park, and John Hwa Lee. "Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid." Acta Veterinaria Hungarica 63, no. 4 (2015): 401–12. http://dx.doi.org/10.1556/004.2015.038.

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Escherichia coli heat-labile enterotoxin B subunit (LTB) protein is a potent adjuvant. Salmonella Gallinarum ghosts carrying LTB (S. Gallinarum-LTB ghosts) were genetically constructed using a plasmid, pJHL187-LTB, designed for the co-expression of the LTB and E lysis proteins. This study evaluates the immunopotentiating effects of Montanide™ ISA 70 VG on S. Gallinarum-LTB ghost vaccination against fowl typhoid. Five-week-old layer chickens were injected intramuscularly with sterile PBS (non-immunised control, Group A), S. Gallinarum-LTB ghost (Group B) or S. Gallinarum-LTB ghost emulsified wi

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Wooden, Jason M., Greg Finney, Michael MacCoss, and Diana M. Gilligan. "Comprehensive Analysis of Murine and Human RBC Ghost Proteomes." Blood 108, no. 11 (2006): 1569. http://dx.doi.org/10.1182/blood.v108.11.1569.1569.

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Abstract Inherited hemolytic anemia (spherocytosis or elliptocytosis) is one of the most common inherited diseases with an incidence of 1:2500 to 1:5000 in populations of Northern European descent. Mild to severe inherited hemolytic anemias can arise from defects in the red blood cell (RBC) membrane skeleton. Genetic knock-out of various components of this apparatus has led to the creation of mouse models which have contributed significantly to our understanding of these disorders in humans. However, the mouse and human RBC protein complements have not been comprehensively compared. Using newl

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Nunes-Correia, Isabel, João Ramalho-Santos, and Maria C. Pedroso de Lima. "Sendai Virus Fusion Activity as Modulated by Target Membrane Components." Bioscience Reports 18, no. 2 (1998): 59–68. http://dx.doi.org/10.1023/a:1020180109275.

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We have studied the differences between erythrocytes and erythrocyte ghosts as target membranes for the study of Sendai virus fusion activity. Fusion was monitored continuously by fluorescence dequenching of R18-labeled virus. Experiments were carried out either with or without virus/target membrane prebinding. When Sendai virus was added directly to a erythrocyte/erythrocyte ghost suspension, fusion was always lower than that obtained when experiments were carried out with virus already bound to the erythrocyte/erythrocyte ghost in the cold, since with virus prebinding fusion can be triggered

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Yang, Chinglai, Qingyuan Yang, and Richard W. Compans. "Coreceptor-Dependent Inhibition of the Cell Fusion Activity of Simian Immunodeficiency Virus Env Proteins." Journal of Virology 74, no. 13 (2000): 6217–22. http://dx.doi.org/10.1128/jvi.74.13.6217-6222.2000.

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ABSTRACT The cytoplasmic tail (R peptide) sequence is able to regulate the fusion activity of the murine leukemia virus (MuLV) envelope (Env) protein. We have previously shown that this sequence exerts a profound inhibitory effect on the fusion activity of simian immunodeficiency virus (SIV)-MuLV chimeric Env proteins which contain the extracellular and transmembrane domains of the SIV Env protein. Recent studies have shown that SIV can utilize several alternative cellular coreceptors for its fusion and entry into the cell. We have investigated the fusion activity of SIV and SIV-MuLV chimeric

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Hopgood, M. F., S. E. Knowles, and F. J. Ballard. "Proteolysis of N-ethylmaleimide-modified aldolase loaded into erythrocyte ghosts: prevention by inhibitors of calpain." Biochemical Journal 259, no. 1 (1989): 237–42. http://dx.doi.org/10.1042/bj2590237.

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1. When rabbit muscle aldolase labelled with tritium and inactivated by N-ethylmaleimide (NEM) was loaded into erythrocyte ghosts, significant proteolysis of the loaded protein occurred. The major product of this proteolysis, separated by electrophoresis under dissociating conditions, was found to be approx. 2 kDa smaller than the parent protein. 2. Proteolysis was detectable during erythrocyte ghost loading at 0 degrees C, reaching a plateau after approx. 12 min. Subsequent incubation at 37 degrees C to allow resealing of the ghosts resulted in additional proteolysis, and up to 20% of the loa

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Turrini, F., A. Naitana, L. Mannuzzu, G. Pescarmona, and P. Arese. "Increased red cell calcium, decreased calcium adenosine triphosphatase, and altered membrane proteins during fava bean hemolysis in glucose-6- phosphate dehydrogenase-deficient (Mediterranean variant) individuals." Blood 66, no. 2 (1985): 302–5. http://dx.doi.org/10.1182/blood.v66.2.302.302.

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Abstract RBCs from four glucose-6-phosphate dehydrogenase (G6PD)-deficient (Mediterranean variant) subjects were studied during fava bean hemolysis. In the density-fractionated RBC calcium level, Ca2+-ATPase activity, reduced glutathione level, and ghost protein pattern were studied. In the bottom fraction, containing most heavily damaged RBCs, calcium level ranged from 143 to 244 mumol/L RBCs (healthy G6PD- deficient controls: 17 +/- 5 mumol/L RBCs). The Ca2+-ATPase activity ranged from 0.87 to 1.84 mumol ATP consumed/g Hb/min (healthy G6PD- deficient controls: 2.27 +/- 0.4). Sodium dodecyl s

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Turrini, F., A. Naitana, L. Mannuzzu, G. Pescarmona, and P. Arese. "Increased red cell calcium, decreased calcium adenosine triphosphatase, and altered membrane proteins during fava bean hemolysis in glucose-6- phosphate dehydrogenase-deficient (Mediterranean variant) individuals." Blood 66, no. 2 (1985): 302–5. http://dx.doi.org/10.1182/blood.v66.2.302.bloodjournal662302.

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RBCs from four glucose-6-phosphate dehydrogenase (G6PD)-deficient (Mediterranean variant) subjects were studied during fava bean hemolysis. In the density-fractionated RBC calcium level, Ca2+-ATPase activity, reduced glutathione level, and ghost protein pattern were studied. In the bottom fraction, containing most heavily damaged RBCs, calcium level ranged from 143 to 244 mumol/L RBCs (healthy G6PD- deficient controls: 17 +/- 5 mumol/L RBCs). The Ca2+-ATPase activity ranged from 0.87 to 1.84 mumol ATP consumed/g Hb/min (healthy G6PD- deficient controls: 2.27 +/- 0.4). Sodium dodecyl sulfate- p

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Cardon, Tristan, Isabelle Fournier, and Michel Salzet. "SARS-Cov-2 Interactome with Human Ghost Proteome: A Neglected World Encompassing a Wealth of Biological Data." Microorganisms 8, no. 12 (2020): 2036. http://dx.doi.org/10.3390/microorganisms8122036.

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Conventionally, eukaryotic mRNAs were thought to be monocistronic, leading to the translation of a single protein. However, large-scale proteomics have led to a massive identification of proteins translated from mRNAs of alternative ORF (AltORFs), in addition to the predicted proteins issued from the reference ORF or from ncRNAs. These alternative proteins (AltProts) are not represented in the conventional protein databases and this “ghost proteome” was not considered until recently. Some of these proteins are functional and there is growing evidence that they are involved in central functions

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Plundrich, Nathalie, Mary Ann Lila, Edward Foegeding, and Scott Laster. "Protein-bound polyphenols create “ghost” band artifacts during chemiluminescence-based antigen detection." F1000Research 6 (March 13, 2017): 254. http://dx.doi.org/10.12688/f1000research.10622.1.

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Antigen detection during Western blotting commonly utilizes a horseradish peroxidase-coupled secondary antibody and enhanced chemiluminescent substrate. We utilized this technique to examine the impact of green tea-derived polyphenols on the binding of egg white protein-specific IgE antibodies from allergic human plasma to their cognate antigens. Our experiments unexpectedly showed that green tea-derived polyphenols, when stably complexed with egg white proteins, caused hyperactivation of horseradish peroxidase resulting in the appearance of white “ghost” bands. This study suggests that cautio

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Plundrich, Nathalie, Mary Ann Lila, Edward Foegeding, and Scott Laster. "Protein-bound polyphenols create “ghost” band artifacts during chemiluminescence-based antigen detection." F1000Research 6 (May 26, 2017): 254. http://dx.doi.org/10.12688/f1000research.10622.2.

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Antigen detection during Western blotting commonly utilizes a horseradish peroxidase-coupled secondary antibody and enhanced chemiluminescent substrate. We utilized this technique to examine the impact of green tea-derived polyphenols on the binding of egg white protein-specific IgE antibodies from allergic human plasma to their cognate antigens. Our experiments unexpectedly showed that green tea-derived polyphenols, when stably complexed with egg white proteins, caused “ghost” band formation in the presence of horseradish peroxide. This study suggests that caution should be taken when evaluat

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Ito, Shota, Hideki Kandori, and Victor A. Lorenz-Fonfria. "Potential Second-Harmonic Ghost Bands in Fourier Transform Infrared (FT-IR) Difference Spectroscopy of Proteins." Applied Spectroscopy 72, no. 6 (2018): 956–63. http://dx.doi.org/10.1177/0003702818757521.

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Fourier transform infrared (FT-IR) difference absorption spectroscopy is a common method for studying the structural and dynamical aspects behind protein function. In particular, the 2800–1800 cm−1 spectral range has been used to obtain information about internal (deuterated) water molecules, as well as site-specific details about cysteine residues and chemically modified and artificial amino acids. Here, we report on the presence of ghost bands in cryogenic light-induced FT-IR difference spectra of the protein bacteriorhodopsin. The presence of these ghost bands can be particularly problemati

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ørntoft, T. F., and N. Clausen. "Hereditary spherocytosis: Diagnostic and anaemia-associated aberrations of ghost proteins." Scandinavian Journal of Clinical and Laboratory Investigation 54, no. 2 (1994): 95–103. http://dx.doi.org/10.3109/00365519409086515.

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Cardon, Tristan, Flore Hervé, Vivian Delcourt, et al. "Optimized Sample Preparation Workflow for Improved Identification of Ghost Proteins." Analytical Chemistry 92, no. 1 (2019): 1122–29. http://dx.doi.org/10.1021/acs.analchem.9b04188.

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Barel, Gilli, Alexandra Sirota, Hanne Volpin, and Edouard Jurkevitch. "Fate of Predator and Prey Proteins during Growth of Bdellovibrio bacteriovorus on Escherichia coli and Pseudomonas syringae Prey." Journal of Bacteriology 187, no. 1 (2005): 329–35. http://dx.doi.org/10.1128/jb.187.1.329-335.2005.

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ABSTRACT A two-dimensional electrophoretic analysis of protein distribution followed by identification of selected proteins by mass spectrometry was performed on fresh bdellovibrio cultures containing attack phase cells of the predatory bacterium Bdellovibrio bacteriovorus strain 109J-1 and the remains of an Escherichia coli or a Pseudomonas syringae pv. tomato prey. Cleavage of the peptidoglycan-associated outer membrane proteins (OMPs) OmpA in E. coli and OprF in P. syringae occurred in both prey. The tryptic peptides obtained from the cleavage products of OmpA and OprF were all located with

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SCHWARTZ, S. Robert, C. Anne RYBICKI, and L. Ronald NAGEL. "Molecular cloning and expression of a chloride channel-associated protein pICln in human young red blood cells: association with actin." Biochemical Journal 327, no. 2 (1997): 609–16. http://dx.doi.org/10.1042/bj3270609.

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We report the cloning and sequencing from human reticulocytes of cDNA coding for the Cl- channel-associated protein, pICln. Human reticulocyte pICln (HRpICln) cDNA encodes a protein (predicted molecular mass 26293 Da) identical with human non-pigmented ciliary epithelial cell pICln. By using full-length HRpICln cDNA (approx. 1.2 kb) to probe human lymphocyte metaphase-chromosome spreads, the location of the human ICln gene was mapped to 11q13 by fluorescence in situ hybridization analysis. Polyclonal antibodies to recombinant HRpICln detected bands at approx. 43 kDa and approx. 37 kDa in both

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Cardon, Tristan, Michel Salzet, Julien Franck, and Isabelle Fournier. "Nuclei of HeLa cells interactomes unravel a network of ghost proteins involved in proteins translation." Biochimica et Biophysica Acta (BBA) - General Subjects 1863, no. 10 (2019): 1458–70. http://dx.doi.org/10.1016/j.bbagen.2019.05.009.

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Xiao, Guipeng, Jintao Lu, Zhende Yang, Hengfei Fu, and Ping Hu. "A Study of Adult Olfactory Proteins of Primitive Ghost Moth, Endoclita signifer (Lepidoptera, Hepialidae)." Life 13, no. 12 (2023): 2264. http://dx.doi.org/10.3390/life13122264.

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Endoclita signifer is a prominent wood-boring insect species in eucalyptus plantations in Guangxi, China, causing significant ecological and economic damage. A novel approach to controlling the challenging wood-boring pest involves disrupting the olfactory communication between insects and the volatile compounds emitted by plants. To identify the olfactory proteins contributing to host selection based on 11 GC-EAD-active volatiles from eucalyptus leaves and to discover the highly expressed olfactory proteins, we conducted a study on the antennal transcriptomes of adult E. signifer and screened

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Bradford, Emily, Gary Shull, and Marian Miller. "The Ghost of the IEL: A Halloween Photoshop Exercise." Microscopy Today 16, no. 6 (2008): 75. http://dx.doi.org/10.1017/s1551929500062465.

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Image of an intraepithelial lymphocyte (IEL) from a CLIC5 mutant mouse small intestine. The CLIC (Chloride Intracellular Channel) family of proteins is expressed in a wide variety of cell types, and several isoforms are known to cycle between soluble and membranebound forms. As well as being widely expressed, the CLICs are involved in diverse functions, including tubulogenesis, immune cell activation, apoptosis and calcium handling. CLIC5 has been shown to associate with cytoskeletal proteins in placental microvilli and inner ear cells, and is required for proper maintenence of hair cell steri

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Wooden, Jason M., Greg L. Finney, Michael J. MacCoss, Luanne L. Peters, and Diana M. Gilligan. "Proteomic Analysis of RBC Ghosts from the Beta-Adducin and Protein 4.2 Knock-Out Mouse Models of Inherited Hemolytic Anemia." Blood 110, no. 11 (2007): 1728. http://dx.doi.org/10.1182/blood.v110.11.1728.1728.

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Abstract Inherited hemolytic anemia (spherocytosis or elliptocytosis) is one of the most common inherited diseases with an incidence of 1:2500 to 1:5000 in populations of Northern European descent. While it is known that mild to severe inherited hemolytic anemias can arise from defects in the red blood cell (RBC) membrane skeleton, fundamental questions remain unanswered surrounding the clinical variability and non-erythroid effects of known RBC membrane skeleton mutations. To identify proteins that may be involved in disease severity and secondary effects, we used shotgun proteomics to global

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Murgoci, Adriana-Natalia, Tristan Cardon, Soulaimane Aboulouard, et al. "Reference and Ghost Proteins Identification in Rat C6 Glioma Extracellular Vesicles." iScience 23, no. 5 (2020): 101045. http://dx.doi.org/10.1016/j.isci.2020.101045.

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Vaziri, C., та C. P. Downes. "G-protein-mediated activation of turkey erythrocyte phospholipase C by β-adrenergic and P2y-purinergic receptors". Biochemical Journal 284, № 3 (1992): 917–22. http://dx.doi.org/10.1042/bj2840917.

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Isoprenaline, previously known only to stimulate adenylate cyclase via the stimulatory G-protein, Gs, activates turkey erythrocyte ghost phospholipase C (PLC) in a dose-dependent manner when GTP or guanosine 5′-[gamma-thio]triphosphate (GTP[S]) is present. The effect is specific in that it is abolished by beta-adrenergic-receptor antagonists. Stimulation of adenosine receptors, which also couple to adenylate cyclase via Gs in turkey erythrocytes, does not activate PLC, indicating that the stimulation observed in the presence of isoprenaline is not due to Gs activation. Furthermore, the stimula

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Hjelm, Anna, Bill Söderström, David Vikström, Wouter S. P. Jong, Joen Luirink, and Jan-Willem de Gier. "Autotransporter-Based Antigen Display in Bacterial Ghosts." Applied and Environmental Microbiology 81, no. 2 (2014): 726–35. http://dx.doi.org/10.1128/aem.02733-14.

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ABSTRACTBacterial ghosts are empty cell envelopes of Gram-negative bacteria that can be used as vehicles for antigen delivery. Ghosts are generated by releasing the bacterial cytoplasmic contents through a channel in the cell envelope that is created by the controlled production of the bacteriophage ϕX174 lysis protein E. While ghosts possess all the immunostimulatory surface properties of the original host strain, they do not pose any of the infectious threats associated with live vaccines. Recently, we have engineered theEscherichia coliautotransporter hemoglobin protease (Hbp) into a platfo

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Malinin, G. I., J. V. Garcia, F. J. Hornicek, W. B. Glew, and T. P. Nigra. "Selective affinity of 8-methoxypsoralen for erythrocyte ghost proteins during UV-A irradiation." Photobiochemistry and Photobiophysics 12, no. 3-4 (1986): 283–88. http://dx.doi.org/10.1016/s0165-8646(24)00372-6.

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Collin, Matthew A., Kazuei Mita, Frantisek Sehnal, and Cheryl Y. Hayashi. "Molecular Evolution of Lepidopteran Silk Proteins: Insights from the Ghost Moth, Hepialus californicus." Journal of Molecular Evolution 70, no. 5 (2010): 519–29. http://dx.doi.org/10.1007/s00239-010-9349-8.

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Plattner, H., R. Pape, B. Haacke, K. Olbricht, C. Westphal, and H. Kersken. "Synchronous exocytosis in Paramecium cells. VI. Ultrastructural analysis of membrane resealing and retrieval." Journal of Cell Science 77, no. 1 (1985): 1–17. http://dx.doi.org/10.1242/jcs.77.1.1.

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After the synchronous induction of exocytosis of secretory organelles (trichocysts) in Paramecium tetraurelia cells the process of membrane resealing and retrieval could be followed under synchronous conditions. The characteristic aggregates of membrane intercalated particles (MIPs) contained within the freeze-fractured cell membrane (rings and rosettes) and trichocyst membranes (annulus MIPs), in addition to collar striations on the top of trichocyst membranes, served as endogenous ultrastructural markers. This allowed us to follow the re-arrangement of membrane constituents during and after

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Żyłka, Romuald, Justyna Kupiec, and Stanislaw Przestalski. "Peptides conformational changes of the erythrocyte membrane induced by organometallic tin compounds." Current Topics in Biophysics 34, no. 1 (2011): 31–35. http://dx.doi.org/10.2478/v10214-011-0005-2.

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Peptides conformational changes of the erythrocyte membrane induced by organometallic tin compoundsThe paper presents the results of a study on the effect of selected organic chlorides of tin on peptide conformations of erythrocyte ghosts from pig blood. The following compounds were used: dibutyltin dichloride (DBT), tributyltin chloride (TBT), diphenyltin dichloride (DPhT) and triphenyltin chloride (TPhT). Peptide conformation changes were determined on the basis of measurements done with the ATR FTIR technique. This method made it possible to measure the percent share of a peptide with speci

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Rybicki, AC, RS Schwartz, EJ Hustedt, and CE Cobb. "Increased rotational mobility and extractability of band 3 from protein 4.2-deficient erythrocyte membranes: evidence of a role for protein 4.2 in strengthening the band 3-cytoskeleton linkage." Blood 88, no. 7 (1996): 2745–53. http://dx.doi.org/10.1182/blood.v88.7.2745.bloodjournal8872745.

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Band 3 (anion-exchange protein 1-[AE1]) is the major integral membrane protein of human erythrocytes and links the membrane to the underlying cytoskeleton via high-affinity binding to ankyrin. It is unclear whether other cytoskeletal proteins participate in strengthening the ankyrin-band 3 linkage, but a putative role for protein 4.2 (P4.2) has been proposed based on the increased osmotic fragility and spherocytic morphology of P4.2-deficient red blood cells (RBCs). The present study was designed to investigate the hypothesis that P4.2 has a direct role in strengthening the band 3-cytoskeleton

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Glowacki, David R., Jeremy N. Harvey, and Adrian J. Mulholland. "Protein dynamics and enzyme catalysis: the ghost in the machine?" Biochemical Society Transactions 40, no. 3 (2012): 515–21. http://dx.doi.org/10.1042/bst20120047.

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One of the most controversial questions in enzymology today is whether protein dynamics are significant in enzyme catalysis. A particular issue in these debates is the unusual temperature-dependence of some kinetic isotope effects for enzyme-catalysed reactions. In the present paper, we review our recent model [Glowacki, Harvey and Mulholland (2012) Nat. Chem. 4, 169–176] that is capable of reproducing intriguing temperature-dependences of enzyme reactions involving significant quantum tunnelling. This model relies on treating multiple conformations of the enzyme–substrate complex. The results

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Hopgood, M. F., S. E. Knowles, J. S. Bond, and F. J. Ballard. "Degradation of native and modified forms of fructose-bisphosphate aldolase microinjected into HeLa cells." Biochemical Journal 256, no. 1 (1988): 81–88. http://dx.doi.org/10.1042/bj2560081.

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The uptake and degradation of radiolabelled rabbit muscle fructose-bisphosphate aldolase (EC 4.1.2.13) was studied in HeLa cells microinjected by the erythrocyte ghost fusion system. Labelled aldolase was progressively modified by treatment with GSSG or N-ethylmaleimide (NEM) before microinjection to determine whether these agents, which inactivate and destabilize the enzyme in vitro, affect the half-life of the enzyme in vivo. Increasing exposure of aldolase to GSSG or NEM before microinjection increased the extent of aldolase transfer into the HeLa cells and decreased the proportion of the p

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Willett, Brian J., Celia A. Cannon, and Margaret J. Hosie. "Upregulation of Surface Feline CXCR4 Expression following Ectopic Expression of CCR5: Implications for Studies of the Cell Tropism of Feline Immunodeficiency Virus." Journal of Virology 76, no. 18 (2002): 9242–52. http://dx.doi.org/10.1128/jvi.76.18.9242-9252.2002.

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ABSTRACT Feline CXCR4 and CCR5 were expressed in feline cells as fusion proteins with enhanced green fluorescent protein (EGFP). Expression of the EGFP fusion proteins was localized to the cell membrane, and surface expression of CXCR4 was confirmed by using a cross-species-reactive anti-CXCR4 monoclonal antibody. Ectopic expression of feline CCR5 enhanced expression of either endogenous feline CXCR4 or exogenous feline or human CXCR4 expressed from a retrovirus vector, indicating that experiments investigating the effect of CCR5 expression on feline immunodeficiency virus (FIV) infection must

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Trindade, I. B., G. Hernandez, E. Lebègue, et al. "Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli." JBIC Journal of Biological Inorganic Chemistry 26, no. 2-3 (2021): 313–26. http://dx.doi.org/10.1007/s00775-021-01854-y.

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AbstractIron is a fundamental element for virtually all forms of life. Despite its abundance, its bioavailability is limited, and thus, microbes developed siderophores, small molecules, which are synthesized inside the cell and then released outside for iron scavenging. Once inside the cell, iron removal does not occur spontaneously, instead this process is mediated by siderophore-interacting proteins (SIP) and/or by ferric-siderophore reductases (FSR). In the past two decades, representatives of the SIP subfamily have been structurally and biochemically characterized; however, the same was no

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Kuross, SA, BH Rank, and RP Hebbel. "Excess heme in sickle erythrocyte inside-out membranes: possible role in thiol oxidation." Blood 71, no. 4 (1988): 876–82. http://dx.doi.org/10.1182/blood.v71.4.876.876.

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Abstract It has been suggested that the development of sickle RBC membrane defects might be related to abnormal amounts of membrane-associated heme (a term we use in its generic sense to include hemoglobins, hemichromes, and free heme). Techniques previously used to measure membrane heme, however, would not distinguish between what is truly membrane-associated and what is merely trapped in RBC ghost preparations. Consequently, we have examined extensively washed inside- out membranes (IOM) prepared from normal and sickle RBC. Approximately 25% of the sickle ghost heme is lost upon conversion t

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Kuross, SA, BH Rank, and RP Hebbel. "Excess heme in sickle erythrocyte inside-out membranes: possible role in thiol oxidation." Blood 71, no. 4 (1988): 876–82. http://dx.doi.org/10.1182/blood.v71.4.876.bloodjournal714876.

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It has been suggested that the development of sickle RBC membrane defects might be related to abnormal amounts of membrane-associated heme (a term we use in its generic sense to include hemoglobins, hemichromes, and free heme). Techniques previously used to measure membrane heme, however, would not distinguish between what is truly membrane-associated and what is merely trapped in RBC ghost preparations. Consequently, we have examined extensively washed inside- out membranes (IOM) prepared from normal and sickle RBC. Approximately 25% of the sickle ghost heme is lost upon conversion to IOM, bu

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Homicek, F. J., G. I. Malinin, W. B. Glew, U. Awret, J. V. Garcia, and T. P. Nigra. "Photochemical cross-linking of erythrocyte ghost proteins in the presence of 8-methoxy and trimethylpsoralens." Photobiochemistry and Photobiophysics 9, no. 4 (1985): 263–69. http://dx.doi.org/10.1016/s0165-8646(24)00324-6.

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MAYNARD, D. M., H. F. G. HEIJNEN, W. A. GAHL та M. GUNAY-AYGUN. "The α-granule proteome: novel proteins in normal and ghost granules in gray platelet syndrome". Journal of Thrombosis and Haemostasis 8, № 8 (2010): 1786–96. http://dx.doi.org/10.1111/j.1538-7836.2010.03932.x.

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Jain, Amisha, Himanshu Dhanodkar, Anjali Shujalpurkar, and Gauri Motiwale. "Calcifying Odontogenic Cyst: An Enigma." International Journal of Orofacial Research 7, no. 2 (2023): 37–41. http://dx.doi.org/10.56501/intjorofacres.v7i2.895.

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The calcifying odontogenic cyst (COC), discovered in 1962, is a rare developmental odontogenic cyst clinically present as slow-growing swelling mainly in the anterior portion of the jaws, generally present in the second and sixth decades of life. It accounts for 0.3%–0.8% of odontogenic cysts. COC showed variations in clinical and radiographic features that are not pathognomic, whereas histomorphology forms exist in 3 patterns: benign cystic, solid (neoplastic), and aggressive (malignant) forms. The radiograph shows well-defined radiolucency with irregular masses, while the histopathologic fea

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Angelescu, Iulia-Roxana, Medana Zamfir, Emanuela-Cătălina Ionetic, and Silvia-Simona Grosu-Tudor. "The Biological Role of the S-Layer Produced by Lactobacillus helveticus 34.9 in Cell Protection and Its Probiotic Properties." Fermentation 10, no. 3 (2024): 150. http://dx.doi.org/10.3390/fermentation10030150.

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Lactobacillus helveticus 34.9 was isolated from a sample of Romanian home-made fermented milk, producing both surface layer proteins and a class III bacteriocin. The present study aimed to investigate the biological and functional role of the S-layer in correlation with its probiotic properties. The presence of S-layer proteins resulted in various degrees of co-aggregation of L. helveticus 34.9 with pathogens and with other lactic acid bacteria, but the removal of these proteins reduced the co-aggregation with all the tested strains. Moreover, the S-layer proved to be involved in cell wall hyd

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May, James M., Zhi-chao Qu, and Charles E. Cobb. "Reduction and uptake of methylene blue by human erythrocytes." American Journal of Physiology-Cell Physiology 286, no. 6 (2004): C1390—C1398. http://dx.doi.org/10.1152/ajpcell.00512.2003.

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A thiazine dye reductase has been described in endothelial cells that reduces methylene blue (MB), allowing its uptake into cells. Because a different mechanism of MB uptake in human erythrocytes has been proposed, we measured MB uptake and reduction in this cell type. Oxidized MB (MB+) stimulated reduction of extracellular ferricyanide in a time- and concentration-dependent manner, reflecting extracellular reduction of the dye. Reduced MB was then taken up by the cells and partially oxidized to MB+. Both forms were retained against a concentration gradient, and their redox cycling induced an

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Beck, K. A., J. A. Buchanan, and W. J. Nelson. "Golgi membrane skeleton: identification, localization and oligomerization of a 195 kDa ankyrin isoform associated with the Golgi complex." Journal of Cell Science 110, no. 10 (1997): 1239–49. http://dx.doi.org/10.1242/jcs.110.10.1239.

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To extend our finding of a Golgi-localized form of the membrane skeleton protein spectrin, we have identified an isoform of ankyrin that associates at steady state with the Golgi complex. Immuno-light and -electron microscopy show that this ankyrin isoform localizes to the perinuclear cytoplasm on tubular vesicular structures that co-stain with Golgi marker proteins. An antiserum raised against erythrocyte ankyrin, which was used to identify the Golgi ankyrin, recognized three prominent polypeptides of 220, 213 and 195 kDa in MDCK cells. Affinity purification of this antiserum against each of

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Homer, Bruce L., Kenneth R. Pierce, Charles H. Bridges, James E. Womack, Blair A. Sowa, and Ramon C. Littell. "Inhibition of Copper-Associated Erythrocyte Ghost Membrane Lipid Peroxidation by Hepatic Cytosolic Low Molecular Weight Proteins." Toxicologic Pathology 19, no. 3 (1991): 206–13. http://dx.doi.org/10.1177/019262339101900302.

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Meng, Qian, Ji‐Hong Zhang, Huan Zhang, et al. "Comparative analysis of C‐type lectin domain proteins in the ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae)." Insect Science 26, no. 3 (2018): 453–65. http://dx.doi.org/10.1111/1744-7917.12564.

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Klei, Thomas, Robin Van Bruggen, Jill Dalimot, et al. "Hemolysis in the Spleen Drives Erythrocyte Turnover." Blood 134, Supplement_1 (2019): 946. http://dx.doi.org/10.1182/blood-2019-124342.

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Erythrocytes circulate for an average of 120 days before they are removed from the circulation. Various processes and factors have been identified that may contribute to degradation of senescent erythrocytes, but this complex process is still not completely understood. Accumulation of removal signals such as phosphatidylserine exposure, changes in CD47 expression and oxidation of proteins and lipids that render them susceptible to complement deposition, may contribute to recognition and degradation by red pulp macrophages (RPM) of the spleen. However, many questions remain on the exact mechani

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Yuan, J., A. Bunyaratvej, S. Fucharoen, C. Fung, E. Shinar, and SL Schrier. "The instability of the membrane skeleton in thalassemic red blood cells." Blood 86, no. 10 (1995): 3945–50. http://dx.doi.org/10.1182/blood.v86.10.3945.bloodjournal86103945.

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The thalassemias are a heterogeneous group of disorders characterized by accumulation either of unmatched alpha or beta globin chains. These in turn cause the intramedullary and peripheral hemolysis that leads to varying anemia. A partial explanation for the hemolysis came our of our studies on material properties that showed that beta-thalassemia (beta- thal) intermedia ghosts were very rigid but unstable. A clue to this instability came from the observation that the spectrin/band 3 ratio was low in red blood cells (RBCs) of splenectomized beta-thal intermedia patients. The possible explanati

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Fye, Haddy KS, Paul Mrosso, Frédéric B. Piel, et al. "Proteomics Pathways of Sickle Cell Anemia (P2SCA): A Comprehensive Analysis By Liquid Chromatography Mass Spectrometry of Erythrocyte Membrane Proteins Characterized from the Muhimbili Sickle Cell Programme, Tanzania." Blood 132, Supplement 1 (2018): 3653. http://dx.doi.org/10.1182/blood-2018-99-114650.

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Abstract Annually, there are 312 000 births with Sickle Cell Anemia (SCA), which has been recognized as one of the most common inherited conditions in Africa and is currently emerging as a condition of prominence in much of the developed world due to migration patterns. Despite its growing importance, there has been a significant lag in the application of emerging methodologies to its research, in particular to support the discovery of disease-associated markers of potential implication in therapeutics and informing a more comprehensive understanding of the condition. To bring SCA in line with

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Mhatre, Radhika D., and Sucheta P. Dandekar. "Evaluation of erythrocyte membrane lipids and proteins in renal disorders." International Journal of Research in Medical Sciences 10, no. 1 (2021): 70. http://dx.doi.org/10.18203/2320-6012.ijrms20214870.

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Background: Membrane lipids and proteins play a significant part in imparting membrane its rheological properties. These parameters are altered in diseased states. Exploring the conformational changes in renal disorders can widen our understanding of its impact on the circulatory system. This could lead to a new diagnostic parameter to study the progress of a disease.Methods: 120 blood samples collected from 30 kidney donors, 30 stage 3-4 Chronic kidney disease (CKD) patients (group 1) and 30 stage 5 CKD patients on dialysis (pre and post dialysis) (group 2) were lysed and washed to obtain ery

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Dubreuil, R. R., and G. B. Bouck. "The membrane skeleton of a unicellular organism consists of bridged, articulating strips." Journal of Cell Biology 101, no. 5 (1985): 1884–96. http://dx.doi.org/10.1083/jcb.101.5.1884.

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In this paper we show that a membrane skeleton associated with the plasma membrane of the unicellular organism Euglena consists of approximately 40 individual S-shaped strips that overlap along their lateral margins. The region of strip overlap is occupied by a set of microtubule-associated bridges and microtubule-independent bridges. Both cell form and plasma membrane organization are dependent on the integrity of this membrane skeleton. Removal of the membrane skeleton with a low-molar base results in loss of membrane form and randomization of the paracrystalline membrane interior characteri

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Ohtani, Naoto, and Makoto Miyata. "Identification of a novel nucleoside triphosphatase from Mycoplasma mobile: a prime candidate motor for gliding motility." Biochemical Journal 403, no. 1 (2007): 71–77. http://dx.doi.org/10.1042/bj20061439.

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A protein with a molecular mass of 42 kDa (P42) from Mycoplasma mobile, one of several mycoplasmas that exhibit gliding motility, was shown to be a novel NTPase (nucleoside triphosphatase). Although the P42 protein lacks a common ATP-binding sequence motif (Walker A), the recombinant proteins expressed in Escherichia coli certainly hydrolysed some nucleoside triphosphates, including ATP. The results of photoaffinity labelling by an ATP analogue supported that the P42 protein contains a specific binding site for ATP (or another nucleoside triphosphate). In the M. mobile genome, the P42 gene is

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Iwaki-Egawa, Sachiko, and Garret M. Ihler. "Comparison of the abilities of proteins from Bartonella bacilliformis and Bartonella henselae to deform red cell membranes and to bind to red cell ghost proteins." FEMS Microbiology Letters 157, no. 1 (2006): 207–17. http://dx.doi.org/10.1111/j.1574-6968.1997.tb12775.x.

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