Relevant bibliographies by topics / Giemsa

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  1. Journal articles
  2. Dissertations / Theses
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  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Giemsa'

Author: Grafiati
Published: 4 June 2021
Last updated: 13 February 2022

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Journal articles on the topic "Giemsa"

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Difford, John. "Giemsa Staining." Journal of Histotechnology 25, no. 2 (June 2002): 123. http://dx.doi.org/10.1179/his.2002.25.2.123.

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Henriques, U. "HAEMATOXYLIN-GIEMSA." Acta Pathologica Microbiologica Scandinavica Section A Pathology 78A, no. 2 (August 15, 2009): 236–37. http://dx.doi.org/10.1111/j.1699-0463.1970.tb00259.x.

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Garbyal, Rajendra S., Neeta Agarwal, and Prachi Kumar. "Leishman-Giemsa Cocktail." Acta Cytologica 50, no. 4 (2006): 403–6. http://dx.doi.org/10.1159/000325981.

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Wittekind, D. H., and V. Kretschmer. "On the nature of Romanowsky-Giemsa staining and the Romanowsky-Giemsa effect. II. A revised Romanowsky-Giemsa staining procedure." Histochemical Journal 19, no. 6-7 (June 1987): 399–401. http://dx.doi.org/10.1007/bf01680459.

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Cochrane, Alexandra, Owen Martin Williams, and Stephen Robinson. "“Look back in giemsa”." Journal of Infection 63, no. 6 (December 2011): 499. http://dx.doi.org/10.1016/j.jinf.2011.04.236.

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Cochrane, Alexandra, Owen Martin Williams, and Stephen Robinson. "“Look back in giemsa”." Journal of Infection 63, no. 6 (December 2011): 505. http://dx.doi.org/10.1016/j.jinf.2011.04.247.

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Peffley, Ellen B., and Jaap N. de Vries. "Giemsa G-Banding inAllium." Biotechnic & Histochemistry 68, no. 2 (January 1993): 83–86. http://dx.doi.org/10.3109/10520299309104671.

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Ardina, Rinny, and Sherly Rosalinda. "Morfologi Eosinofil Pada Apusan Darah Tepi Menggunakan Pewarnaan Giemsa, Wright, dan Kombinasi Wright-Giemsa." Jurnal Surya Medika 3, no. 2 (February 1, 2018): 5–12. http://dx.doi.org/10.33084/jsm.v3i2.91.

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Pemeriksaan apusan darah tepi mampu menilai morfologi sel (eritrosit, leukosit, trombosit), menentukan jumlah dan jenis leukosit, mengestimasi jumlah trombosit dan mengidentifikasi adanya parasit. Pewarnaan Romanowsky adalah pewarnaan yang sering digunakan dan di Indonesia untuk mewarnai preparat apusan darah tepi digunakan pewarnaan Giemsa dan terkadang Wright atau kombinasi Wright-Giemsa. Dalam menilai kualitas apusan darah tepi digunakan penilaian terhadap morfologi eosinofil, karena eosinofil memiliki ciri yang khas, jumlahnya cukup banyak dan mudah diamati. Penelitian ini bertujuan untuk menggambarkan morfologi eosinofil pada apusan darah tepi dengan menggunakan pewarnaan Giemsa, Wright, dan kombinasi Wright-Giemsa. Dilakukan penelitian deskritif kualitatif dengan rancangan penelitian seran lintang (cross sectional study) pada 30 sampel yang diambil dengan teknik Simple Random Sampling. Sampel darah diwarnai dengan Giemsa, Wright, dan kombinasi Wright-Giemsa. Gambaran morfologi eosinofil dengan pewarnaan Giemsa menunjukkan inti sel berwarna biru keunguan dan granula tampak cukup jelas terlihat berwarna merah muda, dan apusan lebih tahan lama setelah disimpan. Pada pewarnaan Wright menunjukan inti sel dan granula tampak lebih jelas terlihat kemerahan dengan warna yang lebih menonjol dibandingkan dengan pewarnaan Giemsa namun kekurangan pewarna Wright yaitu tidak tahan lama dalam iklim tropis. Pada apusan dengan pewarnaan kombinasi Wright-Giemsa terdapat kelebihan dari setiap zat warna dimana granula, plasma dan inti lebih jelas terlihat dan pewarnaan lebih tahan lama disimpan. Namun, perlu diperhatikan juga tujuan dari pembuatan preparat apusan darah tepi, karena apabila ingin menentukan ada/tidaknya parasit akan lebih baik menggunakan pewarnaan Giemsa, sedangkan apabila ingin melihat morfologi basofil akan lebih baik menggunakan pewarnaan Wright.Kata Kunci: Eosinofil, Apusan Darah Tepi, Giemsa, Wright, Kombinasi Wright-Giemsa.
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Moricz, André de, Murilo Melo, Ana Maria Castro, Tercio de Campos, Rodrigo Altenfelder Silva, and Adhemar Monteiro Pacheco Jr. "Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder." Acta Cirurgica Brasileira 25, no. 3 (June 2010): 218–24. http://dx.doi.org/10.1590/s0102-86502010000300002.

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PURPOSE: Establish the prevalence of Helicobacter spp in chronic cholecystitis and its correlation with the gallbladder's histological findings. METHODS: 100 patients were operated for chronic cholecystitis with cholecystolithiasis. In pathological examination of the gallbladder, were evaluated the presence of metaplasia, dysplasia, lymphoid follicles, anaplasia and tumors that might be related to the presence of Helicobacter plus the presence of the bacilli Giemsa? by optical microscopy. From the DNA extracted from the gallbladder's bile, PCR was performed by using specific primers for the identification of Helicobacter spp with amplification of the 400bp segment of rRNA gene16S, with positive control DNA from Helicobacter pylori. All the cases negative for isolation of genetic material were excluded. The cases of PCRΘ and GiemsaΘ were used as negative control group. The histological findings were compared to the presence of bacilli and PCR data using a chi-square and Fisher's Exact test (CI = 95.0%, p <0.05). RESULTS: Of 68 patients, 42 (61.8%) were PCR? for Helicobacter spp and 19 (27.9%) had Giemsa?. There was no correlation between the two findings. The PCR? for Helicobacter spp was not correlated to the histological findings. The presence of lymphoid follicles and metaplasia was related to the Giemsa? (p = 0.025 and p= 0.039). CONCLUSION: There is high prevalence of Helicobacter spp in patients with chronic cholecystitis and cholecystolithiasis without be correlated with the histological patterns studied.
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Hanina, Hanina. "UJI DIAGNOSTIK POLYMERASE CHAIN REACTION DIBANDINGKAN DENGAN PENGECATAN GIEMSA PADA INFEKSI MALARIA." JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" 6, no. 1 (April 4, 2018): 76–86. http://dx.doi.org/10.22437/jmj.v6i1.4823.

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ABSTRACT Plasmodium is a parasite causing malaria, the most important infection disease in the world. Gold standard of malaria diagnosis was founded Plasmodium by Giemsa staining method. Fundamental difference between Giemsa and Polymerase Chain Reaction (PCR) is the ability to detect parasite. Giemsa can detect minimal 50-100 parasit/μl whereas PCR detect parasite DNA in lower parasitemia. The purpose of this study was to determine the sensitivity and specificity of the PCR compared to blood slide with Giemsa in detecting of malaria infection. A diagnostic test has been conducted in Laboratorium Biomedik Fakultas Kedokteran Universitas Jambi and Laboratorium Biomolekuler Fakultas Kedokteran Universitas Sriwijaya. There were 87 subjects who fulfilled the criteria inclusion and drawn by consecutive sampling. Blood samples were taken from venous blood. Detection of Plasmodium used Giemsa and PCR method. Detection of Plasmodium from 87 subjects, Giemsa and PCR method founded 1 subject (1.1%) P. falciparum and 4 subjects (4.6%) P. vivax. 82 subjects (94.3%) were negative. The sensitivity and specificity of PCR was 100%, positive predictive value and negative predictive value was 100%.Conclusion is higher sensitivity and spesificity PCR methode in the malaria diagnosis was proven and PCR methode able to identified Plasmodium species accuratly. Keywords: Plasmodium, Malaria, Giemsa, PCR, Diagnostic Test ABSTRAK Plasmodium merupakan parasit penyebab malaria, suatu penyakit infeksi paling penting di dunia. Baku emas diagnosa malaria adalah menemukan Plasmodium melalui pemeriksaan mikroskopis dengan pengecatan Giemsa. Perbedaan mendasar antara metode Giemsa dengan metode Polymerase Chain Reaction (PCR) terletak pada kemampuan mendeteksi parasit. Metode Giemsa hanya mampu mendeteksi Plasmodium dengan ambang batas antara 50-100 parasit/μl sedangkan metode PCR dapat mendeteksi DNA parasit pada parasitemia yang lebih rendah. Tujuan penelitian ini adalah untuk mengetahui sensitivitas dan spesifisitas metode PCR dibandingkan dengan pengecatan Giemsa dalam menegakkan diagnosis infeksi malaria. Penelitian ini merupakan uji diagnostik yang dilakukan di Laboratorium Biomedik Fakultas Kedokteran dan Ilmu Kesehatan Universitas Jambi dan Laboratorium Biomolekuler Fakultas Kedokteran Universitas Sriwijaya. Penelitian dilakukan mulai bulan Januari sampai April 2017. Subjek penelitian berjumlah 87 orang yang diambil secara consecutive sampling dari laboratorium RS Theresia Jambi. Semua subjek diambil sampel darah venanya, kemudian dilakukan pengecatan Giemsa dan pemeriksaan PCR. Hasil pemeriksaan PCR nested menggunakan primer genus dan primer spesies Plasmodium ditemukan P.falciparum positif sebanyak 1 sampel (1,1%). Sedangkan P.vivax positif sebanyak 4 sampel (4,6%). Sebanyak 82 sampel (94,3%) negatif. Hal ini sesuai dengan hasil pemeriksaan mikroskopis dengan pengecatan Giemsa. Metode PCR dibandingkan dengan metode pengecatan Giemsa sensitivitas dan spesifisitasnya 100%, nilai prediksi positif dan nilai prediksi negatifnya 100%. Dapat disimpulkan bahwa metode PCR sangat sensitif dan spesifik dalam penegakan diagnosis malaria dan mampu mengidentifikasi spesies parasit secara akurat. Kata Kunci: Plasmodium, Malaria, Giemsa, PCR, Uji Diagnostik.
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Dissertations / Theses on the topic "Giemsa"

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Björnsson, Hanna. "Comparison and optimization of May-Grunwald Giemsa and May-Grunwald Giemsa Quick Stain for morphological assessment of pleural and ascites effusions." Thesis, Uppsala universitet, Institutionen för medicinsk cellbiologi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-445869.

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Introduction: Effusion cytology can be performed for the purpose of diagnosis, treatment, and prognosis of malignant disease. A common analysis of effusion cytology samples is the May Grunwald Giemsa stain.    Aim: The aim of the study was to compare May Grunwald Giemsa stain and May Grunwald Giemsa Quick Stain in order to determine the best quality stain and suggest ways to improve the current staining protocol.     Materials and Methods: The methods used in this study are the routine laboratory’s standard procedures for  May-Grunwald Giemsa stain and May-Grunwald Giemsa Quick Stain but with adapted washing steps that investigates the effect of tap water, distilled water, and phosphate buffer on stain quality. Two pleural effusion samples were stained in the initial experiment and two pleural effusions and one ascites sample in the second experiment.    Results and Conclusion: All samples gave a greater score when stained with May-Grunwald Giemsa Quick Stain compared to traditional May-Grunwald Giemsa stain. For the traditional May-Grunwald Giemsa, the use of any of the three phosphate buffers scores higher than the routine washing where tap water is used. In conclusion, it would be of benefit to further investigate and implement phosphate buffer in traditional staining or proceed with the May-Grunwald Quick Stain for all pleural and ascites effusions.
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Giemsa, Artur Verfasser], and Peter [Akademischer Betreuer] [Nielsen. "Aufnahme, Metabolisierung und Toxizität von superparamagnetischen Eisenoxid-Nanopartikeln (SPIOs) / Artur Giemsa. Betreuer: Peter Nielsen." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2013. http://d-nb.info/104502371X/34.

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Frisancho, Talavera Carol Julissa. "Identificación del Helicobacter pylori por el método de coloración Giemsa en biopsias gástricas negativas." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2011. https://hdl.handle.net/20.500.12672/15844.

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La demostración histológica del microorganismo por coloraciones como Giemsa tienen una sensibilidad y especificidad por encima del 90% por lo que sería conveniente utilizar esta coloración en los casos de gastritis crónica con actividad inflamatoria aguda coloreados con el método de rutina Hematoxilina- Eosina, en los cuales no se haya detectado el Helicobacter pylori como era de esperarse. Con el fin de corroborar esta información se realizó el estudio histopatológico de las biopsias gástricas del Servicio de Anatomía Patológica del Hospital Hipólito Unanue en el periodo de julio a diciembre del 2010. Se procedió a revisar las biopsias coloreadas con el método de rutina Hematoxilina- Eosina, que presentaban gastritis crónica activa y Helicobacter Pylori negativo, realizándose en ellas las coloraciones respectivas con el método Giemsa para corroborar o descartar la presencia de la bacteria. El procesamiento de datos se realizó de forma manual con el programa Excel para Windows. Durante los meses de julio a diciembre del año 2010, el número de biopsias gástricas recibidas en el Servicio de Anatomía Patológica del Hospital Nacional Hipólito Unanue de Lima fueron de 1,051. De las 1,051 biopsias se encontró que 381 (36,2%) correspondían a pacientes de sexo masculino y 670 (63,7%) a pacientes de sexo femenino y que la mayor cantidad de biopsias, es decir, 406 (38,6%) correspondían a pacientes cuyas edades oscilaban entre 41 y 60 años. En cuanto al número de fragmentos por lámina se encontró que 625 de ellas (59,5%) presentaban dos fragmentos por cada biopsia y paciente. En cuanto al grado de actividad inflamatoria aguda, se encontró que el 31,2% de estas biopsias no presentaban actividad y que aquellas que presentaban una actividad dentro del grado moderado y leve constituían en conjunto casi el 60% de las biopsias totales. De las 1,051 biopsias gástricas recibidas en el Servicio, se seleccionaron para el presente estudio 97 biopsias. De estas fueron descartadas 54 por no cumplir con los criterios propuestos, quedando 43 biopsias como muestras apropiadas para el estudio. Habiendo sido sometidas estas 43 biopsias al método de coloración Giemsa, y después de haber sido evaluadas al microscopio se encontró que 20 de ellas, es decir el 46,5% presentaron la bacteria, diagnosticándose como Helicobacter pylori positivas con el método de coloración Giemsa. El estudio morfológico de las biopsias gástricas con coloración de Hematoxilina- Eosina es insuficiente para identificar el Helicobacter Pylori en los casos donde se encuentre una gastritis crónica con actividad leve o incluso moderada. Es necesario establecer como un método de rutina la coloración Giemsa, en aquellos casos donde la biopsia gástrica haya sido diagnosticada como gastritis crónica activa con Helicobacter Pylori negativo. Debe coordinarse con el servicio de gastroenterología la posibilidad de obtener un mínimo de 04 biopsias gástricas por cada paciente para así obtener resultados más confiables debido al tipo de infección parcheada que produce el Helicobacter Pylori. Las solicitudes de estudio histopatológico que acompañan a las biopsias remitidas deben ser convenientemente llenadas, con datos adicionales como por ejemplo el antecedente de toma de medicación para erradicar el Helicobacter Pylori, lo que ayudaría a explicar el diagnóstico negativo de la bacteria.
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Giemsa, Esther [Verfasser], and Jucundus [Akademischer Betreuer] Jacobeit. "Haushaltsuntersuchungen der im Alpenraum gemessenen Klimagase CO2 und CH4 / Esther Giemsa ; Betreuer: Jucundus Jacobeit." Augsburg : Universität Augsburg, 2021. http://d-nb.info/1241474346/34.

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Güth, Henrike. "Ein Beitrag zur Blutzellmorphologie ausgewählter karnivorer Zootierarten eine Untersuchung an May-Grünwald-Giemsa-gefärbten Blutausstrichen /." [S.l. : s.n.], 2003. http://dol.uni-leipzig.de/pub/2003-50.

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Praça, Milene Miranda. "Caracterização dos cromossomos de maracujá (Passiflora edulis Sims f. flavicarpa Deg.) com Giemsa, laranja de acridina e FISH." Universidade Federal de Viçosa, 2005. http://www.locus.ufv.br/handle/123456789/10534.

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Passiflora edulis f. flavicarpa é considerada a espécie mais importante do gênero Passiflora, principalmente pelo seu interesse botânico, comercial e em programas de melhoramento. Análises citogenéticas desta espécie revelaram número cromossômico de 2n=18, porém, a caracterização de seus cromossomos tem sido apresentada na literatura com dados conflitantes quanto à posição do centrômero, ao número e localização de constrições secundárias e de regiões organizadoras de nucléolo (RONs). Neste contexto, o objetivo deste trabalho foi adaptar metodologias que ampliassem a resolução das análises cromossômicas em P. edulis f. flavicarpa. Mais especificamente, aplicar técnicas citogenéticas convencionais e moleculares para determinar os aspectos morfológicos dos cromossomos e identificar as RONs. Os meristemas radiculares foram pré-tratados com o bloqueador mitótico Amiprofos metil (APM), 3 μM, durante um período de 16 horas à 4 oC e fixados. As preparações citogenéticas foram realizadas pela técnica de dissociação celular e secagem ao ar e submetidas à coloração convencional com Giemsa, à coloração com o fluorocromo laranja de acridina e à metodologia de FISH (Fluorescent in Situ Hybridization). As figuras cromossômicas foram observadas com objetiva de imersão e capturadas diretamente por uma videocâmera acoplada ao microscópio e a um computador. O bloqueador mitótico utilizado, juntamente com a técnica aplicada, proporcionou acúmulo de células prometafásicas e metafásicas adequadas para análise. Visualizaram-se nove pares de cromossomos bem espalhados nas lâminas, sem sobreposição e com constrições primárias e secundárias definidas, facilitando assim a montagem dos cariogramas. As análises do cariótipo revelaram comprimento médio dos cromossomos metafásicos de 3,75 μm (par 1) a 2,20 μm (par 9) e razões de braços indicando que os cromossomos de 2 a 7 são metacêntricos e o 1, 8 e 9 são submetacêntricos. Observou-se a presença de constrição secundária na porção subterminal do braço longo dos cromossomos 1 e 8 e porção subterminal do braço curto dos cromossomos 2 e 7, sendo que, na literatura, o número dessas constrições variou de um a três em preparações obtidas pela técnica de esmagamento. Com o fluorocromo laranja de acridina, foram observadas regiões emitindo fluorescência verde-amarelada no braço curto do cromossomo 7 e no braço longo do cromossomo 8, evidenciando dois cromossomos que apresentaram constrição secundária. A técnica de FISH, com a utilização de sonda de rDNA 18S, revelou quatro marcações fluorescentes em núcleos interfásicos e marcações fluorescentes nos mesmos pares cromossômicos marcados com laranja de acridina, em metáfase. As marcações obtidas com o fluorocromo correspondem em número e localização dos sítios de rDNA 18S, indicando que somente duas das quatro constrições secundárias encontradas com a coloração de Giemsa estão relacionadas com RON. Assim, considerando que diferenças intraespecíficas e de ecotipos não estejam envolvidas nas diferenças morfológicas pesquisadas, as metodologias aplicadas corroboraram a ampliação da resolução do cariótipo de P. edulis f. flavicarpa. Evidenciou-se, portanto, diferenças na posição do centrômero, número e localização das constrições secundárias e das RONs, quando comparadas aos resultados encontrados na literatura.
Passiflora edulis f. flavicarpa is considered the most important species from the genus Passiflora, mainly because of its botanical and commercial interests, as well as for breeding programs. Cytogenetical analyses of this species have revealed a chromosomic number of 2n=18, however, its chromosome characterization in the literature has been conflicting concerning the centromere position, the number and localization of secondary constrictions and of nucleolus organizer regions (NORs). In this context, the goal of the present work was to adapt methodologies that would amplify the resolution of the chromosomic analyses in P. edulis f. flavicarpa. More especifically, to apply conventional and molecular cytogenetical techniques in order to determine the morphological aspects of the chromosomes and identify the NORs. Root meristems were pre-treated with the mitotic blocker Amiprophos-methyl (APM), 3 μM, for 16 h at 4 °C and then fixated. The cytogenetic preparations were done using the cell dissociation and air-drying technique, followed by the conventional staining with Giemsa, staining with the fluorochrome Acridine Orange and the FISH (Fluorescent in Situ Hybridization) methodology. The chromosomic figures were observed with objective of immersion lenses and captured directly by a videocamera connected to the microscope and to a computer. The employed mitotic blocker, associated to the applied technique, provided the accumulation of prometaphase and metaphase cells adequate to analysis. It was possible to visualize nine pairs of chromosomes well-dispersed on the slides, without overlapping and showing well defined primary and secondary constrictions, so enabling the easier assembly of the xkaryograms. Analyses of the karyotype revealed a mean metaphasic chromosome length ranging from 3.75 μm (pair 1) to 2.20 μm (pair 9) and arm ratios indicating that the chromosomes from 2 to 7 are metacentric, while 1, 8 and 9 are submetacentric. The presence of secondary constrictions was observed on the subterminal portion of the long arm of chromosomes 1 and 8, and on the subterminal portion of the short arm of chromosomes 2 and 7, while in the literature, the number of these constrictions varied from one to three in preparations obtained with the squash technique. With the fluorochrome Acridine Orange, regions were observed emitting green-yellowish fluorescence on the short arm of chromosome 7 and on the long arm of chromosome 8, this way evidencing two chromosomes which presented secondary constriction. The FISH tecnnique, with use of 18S rDNA probe, revealed four fluorescent marks in interphasic nuclei and fluorescent marks on the same chromosome pairs marked in metaphase with Acridine Orange. The marks obtained with the fluorochrome correspond in number and position of the 18S rDNA sites, thus indicating that only two of the four secondary constrictions identified with the Giemsa coloration are related to NOR. Hence, considering that intraspecific and ecotype differences are not involved in the researched morphological differences, the applied methodologies reasserted the resolution amplification of the P. edulis f. flavicarpa karyotype. Therefore, differences on the centromere position, number and localization of the secondary constrictions and of the NORs were verified, when compared to results found on the literature.
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Persson, Gabriella. "Metodutveckling och validering av vätskebaserad teknik på cytologiskt material." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-104592.

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Gonçalves, Juliana. "Características do genoma humano associadas à integração do HIV – análise bioinformática." Master's thesis, Faculdade de Ciências e Tecnologia, 2014. http://hdl.handle.net/10362/11069.

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Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
O vírus da imunodeficiência humana (HIV), para completar o seu ciclo de vida necessita de integrar o seu genoma no genoma humano, sendo que esta integração é feita em locais específicos. Ainda não estão completamente clarificadas as preferências de integração do HIV, por isso o nosso objectivo é estudar estas preferências utilizando as posições de sítios de integração de HIV-1 DNA e HIV-2 DNA isolados de células mononucleadas do sangue periférico (PBMCs) e de HIV-1 DNA isolado de células T Jurkat. As posições dos sítios de integração foram obtidas por recurso a bases de dados. Analisámos se os sítios de integração do HIV coincidiam com as bandas Giemsa claras que, sendo muito activas transcripcionalmente poderiam favorecer a integração. Analisámos também as preferências de integração do HIV relativamente aos sítios frágeis (FSs), alvos preferenciais para a integração de outros vírus. Para este estudo utilizámos dois testes não paramétricos, o dos sinais e de Wilcoxon e uma análise de variância (ANOVA). Os resultados mostraram que o HIV-1 DNA integra com maior intensidade nas bandas Giemsa claras, enquanto que o HIV-2 não apresenta preferências. Já os FSs não constituem alvos preferenciais para a integração deste vírus, integrando o HIV-1 DNA isolado de PBMCs com mais intensidade nas regiões não frágeis. É importante conhecer as preferências de integração do HIV, uma vez que os vectores retrovirais são utilizados em terapia génica, podendo assim contribuir-se para diminuir os riscos associados a esta terapia.
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Nissan, Ramina, and Bella Ombole. "Molek Diffstainer, Aerospray® Hematology Pro och manuell färgning enligt May-Grünwald Giemsa : En jämförande studie med hänseendet till färgningskvaliten, kostnadseffektivitet och tidsbesparing." Thesis, Hälsohögskolan, Högskolan i Jönköping, HHJ. Biomedicinsk plattform, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-31588.

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Wong, Alvaro Yat Set. "´´Evaluación de la compatibilidad de tinciones no fluorescentes de Diffquik, Giemsa, Fastblast y de Feulgen con el Bioensayo Cometa en el ADN espermático humano´´." Bachelor's thesis, Universidad Ricardo Palma, 2016. http://cybertesis.urp.edu.pe/handle/urp/826.

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La fertilidad masculina puede ser medida mediante un espermatograma convencional, sin embargo este examen no incluye la valoración de la integridad del ADN espermático. Esta variable ha sido correlacionada con las tasas de fertilización, viabilidad y desarrollo del embrión, convirtiéndose en una herramienta de importancia clínica tanto para los programas de reproducción animal como los tratamientos de fertilidad asistida. El bioensayo Cometa es capaz de determinar de una manera exacta el valor de la integridad del ADN espermático, lamentablemente este examen no es de uso rutinario por su elevado costo de implementación ya que utiliza microscopia especializada y tinciones fluorescentes para evidenciar la migración del ADN. El objetivo de esta investigación fue evaluar la compatibilidad de las tinciones no fluorescentes Diffquik, Giemsa, de Feulgen y FastBlast en el bioensayo Cometa usando un método visual y automatizado. Se utilizaron 15 eyaculados previamente seleccionados de acuerdo al manual OMS 2010, para luego ser capacitados en búsqueda de homogeneidad adecuada para la experimentación. Cada muestra fue expuesta a una gradiente de Peróxido de hidrogeno (0, 10, 30,60 y 100 mM) por 1 hora a 4°C para luego evaluar el coeficiente de daño mediante el método visual y porcentaje de ADN en la cola mediante el método automatizado. Las pendientes de la regresión lineal en el método visual indican que los valores obtenidos por la tinción control SybrGreen (m=3,69) difieren con Giemsa (m=3,45) y Diffquik (m=2,57). En el método automatizado de igual manera SybrGreen (m=0.83), Giemsa (m=0,79) y Diffquik (m=0,77). Sin embargo SybrGreen es 1,06 veces más efectivo que Giemsa en el visual y 1,05 veces en el automatizado, sugiriendo una compatibilidad con el bioensayo cometa. De igual manera SybrGreen es 1,07 veces más efectivo que Diffquik en el visual y 1,44 veces en el automatizado, concluyendo una compatibilidad solo en el método visual.Male fertility can be measured by a conventional semen analysis, however, this examination does not include the assessment of sperm DNA integrity. This variable has been correlated with fertilization rates, embryo viability and development, becoming a tool of clinical importance for both animal breeding programs and assisted fertility treatments. Comet bioassay is able to determine an exact way the value of sperm DNA integrity, unfortunately this test is not routinely used because of its high cost of implementation because it uses specialized microscopy and fluorescent dyes to demonstrate DNA migration. The objective of this research was to evaluate the compatibility of non-fluorescent dyes Diffquik, Giemsa, Feulgen and Comet FastBlast in the bioassay using a visual and automated method. 15 ejaculates were used previously manually selected according to WHO 2010 and then be trained in finding adequate homogeneity for experimentation. Each sample was exposed to a hydrogen peroxide gradient (0, 10, 30,60 and 100 mM) for 1 hour at 4 ° C and then assess the damage coefficient by visual method and percentage of DNA in the tail by automated method. The slopes of the linear regressions on the visual method indicate that the values obtained by the SybrGreen Control staining (m = 3.69) differ with Giemsa (m = 3.45) and Diffquik (m = 2.57). In the same way automated method SybrGreen (m = 0.83), Giemsa (m = 0.79) and Diffquik (m = 0.77). However SybrGreen is 1.06 times more effective than Giemsa visual and 1.05 times in the automated, suggesting a comet support bioassay. Similarly SybrGreen is 1.07 times more effective than Diffquik visual and 1.44 times in the automated, concluding compatibility only in the visual method. Keywords:
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Books on the topic "Giemsa"

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Baranauskas, Antanas, and Brigita Speičytė. Giesmių giesmė. Vilnius: Vilniaus universiteto leidykla, 2005.

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Friszke, Andrzej. Polska Gierka. Warszawa: Wydawnictwa Szkolne i Pedagogiczne, 1995.

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Kudaba, Č. Žemės giedra. Vilnius: Kultūra, 2004.

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Surowiecki, John. Barney and Gienka. Cincinnati, OH: CW Books, 2010.

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Rurarz, Zdzisław. Byłem doradca̜ Gierka. Chicago: Andy GraFik, 1990.

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Rurarz, Zdzisław. Byłem doradcą Gierka. Chicago: Instytut Wydawniczy Pomost, 1986.

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Hedwig, Saxenhuber, Kunstverein München, Kunsthalle Zürich, and Neue Galerie am Landesmuseum Joanneum., eds. IMI Giese. Stuttgart: Edition Cantz, 1993.

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Marcinkevičius, Justinas. Mažvydas: Trijų dalių giesmė. Vilnius: Scena, 1996.

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Kairys, Anatolijus. Nemarioji giesmė: Istorinis romanas. Chicago, IL: Lietuvių istorijos draugija, 1988.

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Kairys, Anatolijus. Nemarioji giesmė: Istorinis romanas. Chicago, IL: Lietuvių istorijos draugija, 1988.

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Book chapters on the topic "Giemsa"

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Baum, H. "Giemsa-Lösung." In Springer Reference Medizin, 972–73. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_1253.

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Baum, H. "Giemsa-Lösung." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_1253-1.

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Mehlhorn, Heinz. "Giemsa Stain." In Encyclopedia of Parasitology, 1123–24. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-43978-4_1275.

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Mehlhorn, Heinz. "Giemsa Stain." In Encyclopedia of Parasitology, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-642-27769-6_1275-2.

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Kleine, T. O. "Liquor-May-Grünwald-Giemsa-Färbung." In Springer Reference Medizin, 1503. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_1936.

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Kleine, T. O. "Liquor-May-Grünwald-Giemsa-Färbung." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_1936-1.

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Boon, Mathilde E., and Johanna S. Drijver. "Neutral Stains: the Romanowsky — Giemsa Methods." In Routine Cytological Staining Techniques, 90–101. London: Macmillan Education UK, 1986. http://dx.doi.org/10.1007/978-1-349-18250-3_9.

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Stockert, Juan C., Alfonso Blázquez-Castro, and Richard W. Horobin. "Identifying Different Types of Chromatin Using Giemsa Staining." In Methods in Molecular Biology, 25–38. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-706-8_3.

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Bag, Janmejaya, and Monalisa Mishra. "Hemolymph Analysis of Drosophila melanogaster by Giemsa Staining." In Springer Protocols Handbooks, 31–38. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9756-5_3.

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Cho, Jongman. "A Hierarchical Artificial Neural Network Model for Giemsa-Stained Human Chromosome Classification." In 3rd Kuala Lumpur International Conference on Biomedical Engineering 2006, 12–15. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-68017-8_5.

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Conference papers on the topic "Giemsa"

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Malihi, Leila, Karim Ansari-Asl, and Abdolamir Behbahani. "Malaria parasite detection in giemsa-stained blood cell images." In 2013 8th Iranian Conference on Machine Vision and Image Processing (MVIP). IEEE, 2013. http://dx.doi.org/10.1109/iranianmvip.2013.6780011.

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Mushabe, Mark C., Ronald Dendere, and Tania S. Douglas. "Automated detection of malaria in Giemsa-stained thin blood smears." In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. http://dx.doi.org/10.1109/embc.2013.6610346.

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Puttapirat, Pargorn, Montri Phothisonothai, and Suchada Tantisatirapong. "Automated segmentation of erythrocytes from Giemsa-stained thin blood films." In 2016 8th International Conference on Knowledge and Smart Technology (KST). IEEE, 2016. http://dx.doi.org/10.1109/kst.2016.7440503.

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Mani, Manisha S., S. Manisha, K. Palani Thanaraj, and V. Rajinikanth. "Automated segmentation of Giemsa stained microscopic images based on entropy value." In 2017 International Conference on Intelligent Computing, Instrumentation and Control Technologies (ICICICT). IEEE, 2017. http://dx.doi.org/10.1109/icicict1.2017.8342727.

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Preedanan, Wongsakorn, Montri Phothisonothai, Wongwit Senavongse, and Suchada Tantisatirapong. "Automated detection of plasmodium falciparum from Giemsa-stained thin blood films." In 2016 8th International Conference on Knowledge and Smart Technology (KST). IEEE, 2016. http://dx.doi.org/10.1109/kst.2016.7440501.

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S., Edy Victor Haryanto, M. Y. Mashor, A. S. Abdul Nasir, and Zeehaida Mohamed. "Identification of Giemsa Staind of Malaria Using K-Means Clustering Segmentation Technique." In 2018 6th International Conference on Cyber and IT Service Management (CITSM). IEEE, 2018. http://dx.doi.org/10.1109/citsm.2018.8674254.

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Hamghalam, Mohammad, and Ahmad Ayatollahi. "Automatic Counting of Leukocytes in Giemsa-Stained Images of Peripheral Blood Smear." In 2009 International Conference on Digital Image Processing, ICDIP. IEEE, 2009. http://dx.doi.org/10.1109/icdip.2009.9.

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Jericevic, Zeljko, Loris McGavran, and Louis C. Smith. "Digital imaging of Giemsa-banded human chromosomes: eigenanalysis and the Fourier phase reconstruction." In Optics, Electro-Optics, and Laser Applications in Science and Engineering, edited by James E. Boggan, Leonard J. Cerullo, and Louis C. Smith. SPIE, 1991. http://dx.doi.org/10.1117/12.44142.

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Haryanto, S. Edy Victor, M. Y. Mashor, A. S. Abdul Nasir, and H. Jaafar. "Malaria parasite detection with histogram color space method in Giemsa-stained blood cell images." In 2017 5th International Conference on Cyber and IT Service Management (CITSM). IEEE, 2017. http://dx.doi.org/10.1109/citsm.2017.8089291.

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Hamghalam, Mohammad, Mohammad Motameni, and Aghil Esmaeili Kelishomi. "Leukocyte Segmentation in Giemsa-stained Image of Peripheral Blood Smears Based on Active Contour." In 2009 International Conference on Signal Processing Systems. IEEE, 2009. http://dx.doi.org/10.1109/icsps.2009.36.

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Reports on the topic "Giemsa"

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McInerney, Michael K., and John M. Carlyle. : Demonstration of Acoustic Sensing Techniques for Fuel-Distribution System Condition Monitoring : Final Report on Project F07-AR07. Engineer Research and Developmenter Center (U.S.), January 2021. http://dx.doi.org/10.21079/11681/39560.

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Leaks in fuel storage tanks and distribution piping systems have been identified as a mission-critical problem by the Department of Defense and the U.S. Army. Fuel system leaks are often hard to locate and virtually inaccessible for efficient repair because the piping is often installed under a concrete pad or tarmac. Leak repair could cost up to $2,000, and the cost of cleanup and re-mediation for fuel spills can exceed $50,000. In this project an acoustic remote sensing system was installed to monitor an Army heliport refueling system to determine whether it could detect and accurately locate fuel leaks using computer software technolo-gies to distinguish acoustic leakage signatures from normal fuel system operational noise. Demonstration and validation efforts were disadvantaged by the fact that no fuel leaks occurred in the monitored system for the duration of the project. However, the monitoring system did identify several unusual acoustic events within the fueling system and interpret them as indications of intermittent malfunctions of a check valve and a fuel pump. The 30-year ROI is about 6.42. Further work is required before the technology can be fully implemented: its ability to detect fluid leaks must be proven, and the system specifications must be certified through an EPA third party.
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