Journal articles: 'Giemsa'

1

Difford, John. "Giemsa Staining." Journal of Histotechnology 25, no. 2 (June 2002): 123. http://dx.doi.org/10.1179/his.2002.25.2.123.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Henriques, U. "HAEMATOXYLIN-GIEMSA." Acta Pathologica Microbiologica Scandinavica Section A Pathology 78A, no. 2 (August 15, 2009): 236–37. http://dx.doi.org/10.1111/j.1699-0463.1970.tb00259.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Garbyal, Rajendra S., Neeta Agarwal, and Prachi Kumar. "Leishman-Giemsa Cocktail." Acta Cytologica 50, no. 4 (2006): 403–6. http://dx.doi.org/10.1159/000325981.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Wittekind, D. H., and V. Kretschmer. "On the nature of Romanowsky-Giemsa staining and the Romanowsky-Giemsa effect. II. A revised Romanowsky-Giemsa staining procedure." Histochemical Journal 19, no. 6-7 (June 1987): 399–401. http://dx.doi.org/10.1007/bf01680459.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Cochrane, Alexandra, Owen Martin Williams, and Stephen Robinson. "“Look back in giemsa”." Journal of Infection 63, no. 6 (December 2011): 499. http://dx.doi.org/10.1016/j.jinf.2011.04.236.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Cochrane, Alexandra, Owen Martin Williams, and Stephen Robinson. "“Look back in giemsa”." Journal of Infection 63, no. 6 (December 2011): 505. http://dx.doi.org/10.1016/j.jinf.2011.04.247.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Peffley, Ellen B., and Jaap N. de Vries. "Giemsa G-Banding inAllium." Biotechnic & Histochemistry 68, no. 2 (January 1993): 83–86. http://dx.doi.org/10.3109/10520299309104671.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Ardina, Rinny, and Sherly Rosalinda. "Morfologi Eosinofil Pada Apusan Darah Tepi Menggunakan Pewarnaan Giemsa, Wright, dan Kombinasi Wright-Giemsa." Jurnal Surya Medika 3, no. 2 (February 1, 2018): 5–12. http://dx.doi.org/10.33084/jsm.v3i2.91.

Full text

Abstract:

Pemeriksaan apusan darah tepi mampu menilai morfologi sel (eritrosit, leukosit, trombosit), menentukan jumlah dan jenis leukosit, mengestimasi jumlah trombosit dan mengidentifikasi adanya parasit. Pewarnaan Romanowsky adalah pewarnaan yang sering digunakan dan di Indonesia untuk mewarnai preparat apusan darah tepi digunakan pewarnaan Giemsa dan terkadang Wright atau kombinasi Wright-Giemsa. Dalam menilai kualitas apusan darah tepi digunakan penilaian terhadap morfologi eosinofil, karena eosinofil memiliki ciri yang khas, jumlahnya cukup banyak dan mudah diamati. Penelitian ini bertujuan untuk menggambarkan morfologi eosinofil pada apusan darah tepi dengan menggunakan pewarnaan Giemsa, Wright, dan kombinasi Wright-Giemsa. Dilakukan penelitian deskritif kualitatif dengan rancangan penelitian seran lintang (cross sectional study) pada 30 sampel yang diambil dengan teknik Simple Random Sampling. Sampel darah diwarnai dengan Giemsa, Wright, dan kombinasi Wright-Giemsa. Gambaran morfologi eosinofil dengan pewarnaan Giemsa menunjukkan inti sel berwarna biru keunguan dan granula tampak cukup jelas terlihat berwarna merah muda, dan apusan lebih tahan lama setelah disimpan. Pada pewarnaan Wright menunjukan inti sel dan granula tampak lebih jelas terlihat kemerahan dengan warna yang lebih menonjol dibandingkan dengan pewarnaan Giemsa namun kekurangan pewarna Wright yaitu tidak tahan lama dalam iklim tropis. Pada apusan dengan pewarnaan kombinasi Wright-Giemsa terdapat kelebihan dari setiap zat warna dimana granula, plasma dan inti lebih jelas terlihat dan pewarnaan lebih tahan lama disimpan. Namun, perlu diperhatikan juga tujuan dari pembuatan preparat apusan darah tepi, karena apabila ingin menentukan ada/tidaknya parasit akan lebih baik menggunakan pewarnaan Giemsa, sedangkan apabila ingin melihat morfologi basofil akan lebih baik menggunakan pewarnaan Wright.Kata Kunci: Eosinofil, Apusan Darah Tepi, Giemsa, Wright, Kombinasi Wright-Giemsa.

APA, Harvard, Vancouver, ISO, and other styles

9

Moricz, André de, Murilo Melo, Ana Maria Castro, Tercio de Campos, Rodrigo Altenfelder Silva, and Adhemar Monteiro Pacheco Jr. "Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder." Acta Cirurgica Brasileira 25, no. 3 (June 2010): 218–24. http://dx.doi.org/10.1590/s0102-86502010000300002.

Full text

Abstract:

PURPOSE: Establish the prevalence of Helicobacter spp in chronic cholecystitis and its correlation with the gallbladder's histological findings. METHODS: 100 patients were operated for chronic cholecystitis with cholecystolithiasis. In pathological examination of the gallbladder, were evaluated the presence of metaplasia, dysplasia, lymphoid follicles, anaplasia and tumors that might be related to the presence of Helicobacter plus the presence of the bacilli Giemsa? by optical microscopy. From the DNA extracted from the gallbladder's bile, PCR was performed by using specific primers for the identification of Helicobacter spp with amplification of the 400bp segment of rRNA gene16S, with positive control DNA from Helicobacter pylori. All the cases negative for isolation of genetic material were excluded. The cases of PCRΘ and GiemsaΘ were used as negative control group. The histological findings were compared to the presence of bacilli and PCR data using a chi-square and Fisher's Exact test (CI = 95.0%, p <0.05). RESULTS: Of 68 patients, 42 (61.8%) were PCR? for Helicobacter spp and 19 (27.9%) had Giemsa?. There was no correlation between the two findings. The PCR? for Helicobacter spp was not correlated to the histological findings. The presence of lymphoid follicles and metaplasia was related to the Giemsa? (p = 0.025 and p= 0.039). CONCLUSION: There is high prevalence of Helicobacter spp in patients with chronic cholecystitis and cholecystolithiasis without be correlated with the histological patterns studied.

APA, Harvard, Vancouver, ISO, and other styles

10

Hanina, Hanina. "UJI DIAGNOSTIK POLYMERASE CHAIN REACTION DIBANDINGKAN DENGAN PENGECATAN GIEMSA PADA INFEKSI MALARIA." JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" 6, no. 1 (April 4, 2018): 76–86. http://dx.doi.org/10.22437/jmj.v6i1.4823.

Full text

Abstract:

ABSTRACT Plasmodium is a parasite causing malaria, the most important infection disease in the world. Gold standard of malaria diagnosis was founded Plasmodium by Giemsa staining method. Fundamental difference between Giemsa and Polymerase Chain Reaction (PCR) is the ability to detect parasite. Giemsa can detect minimal 50-100 parasit/μl whereas PCR detect parasite DNA in lower parasitemia. The purpose of this study was to determine the sensitivity and specificity of the PCR compared to blood slide with Giemsa in detecting of malaria infection. A diagnostic test has been conducted in Laboratorium Biomedik Fakultas Kedokteran Universitas Jambi and Laboratorium Biomolekuler Fakultas Kedokteran Universitas Sriwijaya. There were 87 subjects who fulfilled the criteria inclusion and drawn by consecutive sampling. Blood samples were taken from venous blood. Detection of Plasmodium used Giemsa and PCR method. Detection of Plasmodium from 87 subjects, Giemsa and PCR method founded 1 subject (1.1%) P. falciparum and 4 subjects (4.6%) P. vivax. 82 subjects (94.3%) were negative. The sensitivity and specificity of PCR was 100%, positive predictive value and negative predictive value was 100%.Conclusion is higher sensitivity and spesificity PCR methode in the malaria diagnosis was proven and PCR methode able to identified Plasmodium species accuratly. Keywords: Plasmodium, Malaria, Giemsa, PCR, Diagnostic Test ABSTRAK Plasmodium merupakan parasit penyebab malaria, suatu penyakit infeksi paling penting di dunia. Baku emas diagnosa malaria adalah menemukan Plasmodium melalui pemeriksaan mikroskopis dengan pengecatan Giemsa. Perbedaan mendasar antara metode Giemsa dengan metode Polymerase Chain Reaction (PCR) terletak pada kemampuan mendeteksi parasit. Metode Giemsa hanya mampu mendeteksi Plasmodium dengan ambang batas antara 50-100 parasit/μl sedangkan metode PCR dapat mendeteksi DNA parasit pada parasitemia yang lebih rendah. Tujuan penelitian ini adalah untuk mengetahui sensitivitas dan spesifisitas metode PCR dibandingkan dengan pengecatan Giemsa dalam menegakkan diagnosis infeksi malaria. Penelitian ini merupakan uji diagnostik yang dilakukan di Laboratorium Biomedik Fakultas Kedokteran dan Ilmu Kesehatan Universitas Jambi dan Laboratorium Biomolekuler Fakultas Kedokteran Universitas Sriwijaya. Penelitian dilakukan mulai bulan Januari sampai April 2017. Subjek penelitian berjumlah 87 orang yang diambil secara consecutive sampling dari laboratorium RS Theresia Jambi. Semua subjek diambil sampel darah venanya, kemudian dilakukan pengecatan Giemsa dan pemeriksaan PCR. Hasil pemeriksaan PCR nested menggunakan primer genus dan primer spesies Plasmodium ditemukan P.falciparum positif sebanyak 1 sampel (1,1%). Sedangkan P.vivax positif sebanyak 4 sampel (4,6%). Sebanyak 82 sampel (94,3%) negatif. Hal ini sesuai dengan hasil pemeriksaan mikroskopis dengan pengecatan Giemsa. Metode PCR dibandingkan dengan metode pengecatan Giemsa sensitivitas dan spesifisitasnya 100%, nilai prediksi positif dan nilai prediksi negatifnya 100%. Dapat disimpulkan bahwa metode PCR sangat sensitif dan spesifik dalam penegakan diagnosis malaria dan mampu mengidentifikasi spesies parasit secara akurat. Kata Kunci: Plasmodium, Malaria, Giemsa, PCR, Uji Diagnostik.

APA, Harvard, Vancouver, ISO, and other styles

11

Imbert, M., and M. El Khoury. "Coloration de May-Grünwald-Giemsa." EMC - Biologie médicale 1, no. 4 (January 2006): 1–6. http://dx.doi.org/10.1016/s2211-9698(06)71347-4.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

HARASHIMA, Saburo. "On the historical review of the Giemsa's, May-Gruenwald-Giemsa and Wright's stain." Journal of the Japanese Society of Clinical Cytology 25, no. 4 (1986): 602–9. http://dx.doi.org/10.5795/jjscc.25.602.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Babic, Tatjana, Hakija Basic, Biljana Selimovic-Miljkovic, Branislava Kocic, and Gordana Tasic. "Detection of Helicobacter pylori in gastric biopsy and resection specimens." Vojnosanitetski pregled 62, no. 1 (2005): 39–43. http://dx.doi.org/10.2298/vsp0501039b.

Full text

Abstract:

Aim. To compare the sensitivity of detecting H. pylori in gastric biopsy and resection specimens using modified Giemsa stain and immunohistochemistry, using a commercially available anti-H. pylori antibody (Dako, Denmark). Methods. Gastric antral biopsy specimens showing chronic gastritis (28 cases) together with tissue blocks from gastrectomy specimens for duodenal ulcer (2 cases) were stained with modified Giemsa and immunoenzymatic alkaline phosphatase - anti-alkaline phosphatase (APAAP) method, and were carefully examined for the presence of H. pylori. Results. Using a modified Giemsa stain, the spiral shaped bacteria of H. pylori stained blue, were attached to the brush border of the gastric foveolar epithelial cells. However, the specificity of modified Giemsa stain depended on the morphological appearance of H. pylori. The specificity of immunostaining permitted detection of low numbers or even single organisms. In all cases bacteria were more prominent and easier to detect in immunostained preparations. H. pylori was identified in 22 (73.3%) of 30 sections stained with modified Giemsa stain, but it could be identified with greater frequency in sections stained with APAAP, in 27 (90%) of 30 sections. Conclusion. Immunohistochemical identification of H. pylori was better than Giemsa stain for detecting that organism.

APA, Harvard, Vancouver, ISO, and other styles

14

Ridhawati, Ridhawati, Riva Ambardina Pradita, Mulyati Mulyati, and Robiatul Adawiyah. "Diagnosis Cepat Kandidemia Neonatus dengan Pemeriksaan Spesimen Darah Pulasan Giemsa." Jurnal Ilmu Kedokteran 13, no. 2 (September 1, 2019): 16. http://dx.doi.org/10.26891/jik.v13i2.2019.16-20.

Full text

Abstract:

Candidemia is one of the major problems in neonates with low birth weight (LBW). Neonatal candidemia has a high rateof morbidity and mortality. Early initiation of antifungal therapy could be decreasing the mortality, but this managementoften hampered due to late diagnosis. The gold standard for diagnosing candidemia is blood culture, but it takes a longtime about 5 days. A rapid alternative method is needed to decrease candidemia morbidity and mortality. The methodchosen in this study is Giemsa stain of blood smear which result could be read within one hour. The study wasconducted in the Department of Parasitology, Faculty of Medicine Universitas Indonesia with a total blood samples of170 from 2009-2012. Blood samples are devided in two, first is made thick blood smear than stain with giemsa, than readby microscope by 400×and 1000× magnification. The second is cultured on Sabauraud agar media and incubated inroom temperature for ten days. Thirty four patients (20%) were positive for Candida, 28 (82.4%) positive samples withboth giemsa staining and culture, while 6 (17,6%) positive culture samples but negative giemsa staining. The values ofthe sensitivity and specificity of the Giemsa stained blood smear examination were 82%, and 100%. This result showsthat Giemsa staining has a good diagnostic value for detecting neonatal candidemia.

APA, Harvard, Vancouver, ISO, and other styles

15

Perea Sasiaín, José. "Cien años del colorante de Giemsa." Biomédica 23, no. 1 (March 1, 2003): 5. http://dx.doi.org/10.7705/biomedica.v23i1.1193.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Woronzoff-Dashkoff, Kristine Krafts. "The Wright-Giemsa stain: Secrets revealed." Clinics in Laboratory Medicine 22, no. 1 (March 2002): 15–23. http://dx.doi.org/10.1016/s0272-2712(03)00065-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Drozdowski, B., and D. A. Mehregan. "Acid-Orcein Giemsa: A Multifunctional Stain." Journal of Cutaneous Pathology 32, no. 1 (June 28, 2008): 86. http://dx.doi.org/10.1111/j.0303-6987.2005.320bm.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Pettenati, M. J. "Giemsa C-banding of Rhoeo (Commelinaceae)." Genetica 74, no. 3 (October 1987): 219–24. http://dx.doi.org/10.1007/bf00056117.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

LINDE-LAURSEN, IB. "Giemsa C-banding of barley chromosomes." Hereditas 88, no. 1 (February 12, 2009): 55–64. http://dx.doi.org/10.1111/j.1601-5223.1978.tb01603.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

LINDE-LAURSEN, IB. "Giemsa C-banding of barley chromosomes." Hereditas 89, no. 1 (February 12, 2009): 37–41. http://dx.doi.org/10.1111/j.1601-5223.1978.tb00978.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

LINDE-LAURSEN, IB. "Giemsa C-banding of barley chromosomes." Hereditas 108, no. 1 (February 14, 2008): 65–76. http://dx.doi.org/10.1111/j.1601-5223.1988.tb00683.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

LINDE-LAURSEN, IB. "Giemsa C-banding of barley chromosomes." Hereditas 100, no. 2 (February 14, 2008): 219–23. http://dx.doi.org/10.1111/j.1601-5223.1984.tb00122.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Crossen, Peter E. "Giemsa banding patterns of human chromosomes*." Clinical Genetics 3, no. 3 (April 23, 2008): 169–79. http://dx.doi.org/10.1111/j.1399-0004.1972.tb01455.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Moscone, Eduardo A., Maria Lambrou, Armando T. Hunziker, and Friedrich Ehrendorfer. "Giemsa C-banded karyotypes inCapsicum (Solanaceae)." Plant Systematics and Evolution 186, no. 3-4 (1993): 213–29. http://dx.doi.org/10.1007/bf00940799.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Shapiro, Howard M., and Francis Mandy. "Cytometry in malaria: Moving beyond Giemsa." Cytometry Part A 71A, no. 9 (2007): 643–45. http://dx.doi.org/10.1002/cyto.a.20453.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Fagiani, M. A. B., A. Fluminhan, E. Araújo, and L. S. L. S. Reis. "Modified comet assay with Giemsa staining: a suitable method for assessing genotoxicity in rats." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 72, no. 3 (May 2020): 853–61. http://dx.doi.org/10.1590/1678-4162-11523.

Full text

Abstract:

ABSTRACT The present study tested a comet assay that was modified for compatibility with Giemsa staining to assess the drug genotoxicity in the peripheral blood of rats. We analysed the peripheral blood of 16 female Wistar rats (N=8 rats/group) from a control group and from a group that was treated with an intraperitoneal injection of 50mg cyclophosphamide/kg. The comet assay was carried out with modifications of the blood volume and immersion time in the lysing solution and different combinations of electrophoresis conditions (running time, voltage and current), to Giemsa staining. The lysing time and electrophoresis conditions allowed for the expression of all classes of DNA damage during the electrophoresis run, and the comets were efficiently stained with Giemsa. The technique showed high reproducibility for the DNA classes. The results demonstrate that the modified comet assay with Giemsa staining can be standardized for routine laboratory procedures using a 20µL blood sample, 3h and 30min immersions in the lysing solution and electrophoresis runs with 23 to 25 V and 310 and 360mA of electrical current. The modified comet assay with Giemsa staining that was described in the present study was standardized to be applied in the laboratory routine.

APA, Harvard, Vancouver, ISO, and other styles

27

Jang, Jin Woo, Ju Yeon Kim, Jung Yoon, Soo Young Yoon, Chi Hyun Cho, Eun Taek Han, Seong Soo A. An, and Chae Seung Lim. "Flow Cytometric Enumeration of Parasitemia in Cultures ofPlasmodium falciparumStained with SYBR Green I and CD235A." Scientific World Journal 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/536723.

Full text

Abstract:

A flow cytometric (FACS) detection method forPlasmodium falciparumcultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The culturedP. falciparumwere spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation betweenP. falciparuminfected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2–37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2–66.0%). High correlation of measured parasitaemia (r=0.98,P<0.05) was observed between FACS and Giemsa stained microscopic analyses. The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis. The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients.

APA, Harvard, Vancouver, ISO, and other styles

28

Omer, Hajir Mohammed Hussien, Khalid Eltahir Khalid, Elhadi Ibrahim Miskeen, Madiha Yousif Taha, Eylaf Yasir Saleh, Elhadi A. Ahmed, Omaima Hassan Abdelwahid, Mohammed Abdelssalam Hassan, and Adam Dawoud Abakar. "Cytological and molecular screening of Chlamydia trachomatis in infertile women attending a maternity teaching hospital in Gezira State, Sudan: a cross-sectional study." F1000Research 9 (June 11, 2020): 589. http://dx.doi.org/10.12688/f1000research.23490.1.

Full text

Abstract:

Background: Chlamydia trachomatis (CT) is a sexually transmitted pathogen that threatens reproductive health worldwide. This study aims to screen CT urogenital infection using cytology and molecular methods in women suffering infertility. Methods: In total, 415 women suffering infertility, attending Wad Madani Maternity Hospital were included in this study and then classified into two groups: primary infertile women and secondary infertile women. Both urine (n= 415) and vaginal swab samples (n= 130) were collected and tested using Giemsa stain and Polymerase Chain Reaction (PCR) for detection of CT. Results: CT was detected in 33.7% (140/415) of urine samples and 73.1% (95/130) of vaginal swab samples using Giemsa stain, compared with 44.6% (185/415) and 84.6% (110/130) using PCR, respectively. In the primary infertile group (n= 265), chlamydia was detected in 35.8% (95/265) of urine and 75% (60/80) of swab samples by Giemsa stain compared with 50.9% (135/265) and 75% (60/80) of the samples by PCR. In the secondary infertile group (n= 150), chlamydia was detected in 30% (45/150) of urine and 70% (35/50) of swab samples by Giemsa stain compared with 33.3% (50/150) and 100% (50/50) of the samples by PCR. The associated risk factors were age, lower abdominal pain, and urethritis (p< 0.05). The sensitivity, specificity, positive predictive value, and negative predictive value of Giemsa stain in detecting chlamydia compared to PCR were 86.4%, 100%, 100%, and 83.6%, respectively. Conclusions: Giemsa stain can be used as a screening test for detection of urogenital chlamydia in urine and vaginal samples in places where PCR is difficult to be performed.

APA, Harvard, Vancouver, ISO, and other styles

29

Labory, Cláudia Regina Gontijo, Eustáquio Souza Dias, Lisete Chamma Davide, Rosane Freitas Schawan, and Inajá Marchizeli Wenzel. "Coloração de núcleos de esporos e hifas do cogumelo Agaricus blazei." Ciência e Agrotecnologia 27, no. 2 (April 2003): 471–74. http://dx.doi.org/10.1590/s1413-70542003000200030.

Full text

Abstract:

Com o objetivo de se estudar o comportamento nuclear durante o ciclo sexual do cogumelo Agaricus blazei , técnicas de coloração e a utilização de fluorocromos foram testadas, visando a uma padronização metodológica para estudos citogenéticos futuros. Das técnicas avaliadas neste trabalho (método de Feulgen, coloração com Giemsa e fluorescência-DAPI), a coloração com Giemsa e o fluorocromo DAPI apresentaram os melhores resultados para evidenciar núcleos. O corante Giemsa permitiu a visualização de compartimentos multinucleados. Com o DAPI, não foi possível a visualização dos septos, sendo necessária a utilização adicional de calcofluor, que tem a propriedade de corar a quitina que delimita os compartimentos das hifas.

APA, Harvard, Vancouver, ISO, and other styles

30

Ali Fadhil Hashim. "Detection of duodenal giardia using giemsa stain." International Journal of Research in Pharmaceutical Sciences 10, no. 2 (April 23, 2019): 1456–59. http://dx.doi.org/10.26452/ijrps.v10i2.717.

Full text

Abstract:

The objectives of this study are to evaluate the benefit of using Giemsa staining in endoscopic duodenal biopsies if it provides an appropriate tool to diagnose giardia parasite more accurate than the usual hematoxylin-eosin stain. Method: a retrospective study including fifty patients have been nominated with upper abdominal pain, attended gastrointestinal tract(GIT) consultation unit in medical City of Imam Al-Hussein-Karbala/ Iraq complaining from upper abdominal discomfort. They have been examined by esophagio- gastro duodenoscopy. All duodenal biopsies have also been collected from pathology excluding all celiac disease cases. All biopsies have paraffin and stained with hematoxylin and eosin looking for Giardia; in the following, the slides are stained with Giemsa stain following the manufacture-instruction. Results: 50patients (27 females and 23 males) have been examined depending on the hematoxylin and eosin staining, respectively, the diagnosis is non-specific chronic duodenitis with low MARSH score. Eleven patients from total fifty-two ones (21.15%) have shown positive symptoms for giardia cyst and trophozoite using the Giemsa stain. Conclusion: Giardia infection is difficult to be diagnosed with hematoxylin-eosin stain, especially with low infection. Consequently, Giemsa stain might be used to increase the efficacy of histopathological diagnosis of the parasite.

APA, Harvard, Vancouver, ISO, and other styles

31

Barcia, Juan José. "The Giemsa Stain: Its History and Applications." International Journal of Surgical Pathology 15, no. 3 (July 2007): 292–96. http://dx.doi.org/10.1177/1066896907302239.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Fominaya, A., C. Vega, and E. Ferrer. "Giemsa C-banded karyotypes of Avena species." Genome 30, no. 5 (October 1, 1988): 627–32. http://dx.doi.org/10.1139/g88-106.

Full text

Abstract:

Giemsa C-banding was used to identify individual somatic chromosomes in eight diploid species of Avena. Two patterns of heterochromatin distribution were found. The chromosomes of five A genome species (A. strigosa, A. hirtula, A. longiglumis, A. damascena, and A. canariensis) possessed mainly telomeric bands, whereas those from three C genome species (A. clauda, A. pilosa, and A. ventricosa) were characterized by higher chromatin condensation and several intercalary heterochromatin bands. The divergent evolution between the two groups is confirmed after C-banding. The unique C-banding patterns of several chromosomes in each species will be a useful tool for the study of meiotic behaviour in interspecific hybrids among Avena spp.Key words: C-banding, Avena, heterochromatin.

APA, Harvard, Vancouver, ISO, and other styles

33

Wittekind, Dietrich, Erik Schulte, Gudrun Schmidt, and GÜNter Frank. "The Standard Romanowsky-Giemsa Stain in Histology." Biotechnic & Histochemistry 66, no. 6 (January 1991): 282–95. http://dx.doi.org/10.3109/10520299109109989.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

LOO, W., M. URIBE-ALCOCER, and J. SCHRÖDER. "The Giemsa-banded karyotype of Romerolagus diazi." Hereditas 91, no. 2 (February 12, 2009): 215–18. http://dx.doi.org/10.1111/j.1601-5223.1979.tb01663.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

NOKKALA, SEPPO. "Staining the centrioles with Feulgen and Giemsa." Hereditas 99, no. 1 (June 28, 2008): 161–63. http://dx.doi.org/10.1111/j.1601-5223.1983.tb00743.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Tsouloufi, Theodora K., Ioannis A. Tsakmakidis, Eleni Tzika, and Maria Kritsepi‐Konstantinou. "Spermatozoa under the microscope: Moving beyond Giemsa." Veterinary Clinical Pathology 49, no. 4 (December 2020): 543–44. http://dx.doi.org/10.1111/vcp.12927.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Niyibizi, Jean Baptiste, and Emmanuel Kamana Gatera. "Diagnostic Performance between Histidine-Rich Protein 2 (HRP-2), a Rapid Malaria Diagnostic Test and Microscopic-Based Staining Techniques for Diagnosis of Malaria." Journal of Tropical Medicine 2020 (March 27, 2020): 1–6. http://dx.doi.org/10.1155/2020/5410263.

Full text

Abstract:

Malaria presents a diagnostic challenge in most tropical countries such as Rwanda. Microscopy remains the gold standard for diagnosing malaria, but it is labor intensive and depends upon the skill of the examiner. Malaria rapid diagnostic tests (RDTs) have been developed as an easy, convenient alternative to microscopy. This cross-sectional study was conducted at Rukara Health Center which is located in Eastern Province, Kayonza district, Rwanda. One hundred and fifty suspected cases of malaria, who attended Rukara Health Centre, during the period, from 21st June to 30th July 2018, were included in this study. HRP-2 RDTs (CareStart™ Malaria HRP-2 (Access Bio, Inc., Somerset, New Jersey, USA)), for malaria were performed. Thick smears were prepared and Giemsa-stained as recommended; then slides were observed under microscopy and reported quantitatively; RDTs were reported qualitatively (positive or negative). Both RDTs and thick smear results were recorded on data collection sheet. This study included a total of 150 study participants, 87 (58%) females and 63 (42%) males. The patients included in the study did not receive any antimalarial drug. The mean age of the study participants was 31.6 ± 12.4 with the majority of participants being between 25 and 44 years and the minority being above 65 years. The sensitivity of RDT (HRP-2) was calculated and found to be 95.0%, whereas the sensitivity of Giemsa microscopy was 100%. The specificity of RDT (HRP-2) was calculated and found to be 59.2%, whereas the specificity of Giemsa microscopy was 100%. Negative and positive predictive values of RDT are 85.4% and 82.7%, respectively. Negative and positive predictive values of Giemsa microscopy were both 100%. According to the results of the current study, the sensitivity, specificity, and both positive and negative predictive values of Giemsa microscopy are higher than those of histidine-rich protein 2-based rapid diagnostic test for malaria. The results obtained in histidine-rich protein 2-based rapid diagnostic test for malaria parasites should be confirmed with tests with high specificity. Further studies should determine the most appropriate type of rapid diagnostic test of malaria diagnosis to be used in combination with Giemsa microscopy. In addition, sensitivity and specificity of RDT (HRP-2) and Giemsa microscopy should be assessed against molecular biology techniques.

APA, Harvard, Vancouver, ISO, and other styles

38

Muthusamy, Swapna, and Selvi Elangovan. "A Study on the Prevalence of Genital Trichomoniasis among Female Outpatients Attending Sexually Transmitted Infection Clinic in a Tertiary Care Hospital." Journal of Laboratory Physicians 9, no. 01 (January 2017): 016–19. http://dx.doi.org/10.4103/0974-2727.187920.

Full text

Abstract:

ABSTRACT Introduction: Women with high-risk sexual behavior accounts for more than half of the sexually transmitted infection (STI) clinic attendees. The prevalence of trichomoniasis is as low as 5% in the general population to as high as 60% in high-risk population. This infection can pave the way to the acquisition of human immunodeficiency virus and other STIs, vice versa and is even associated with cancer. Objectives: To identify, isolate and study the prevalence of Trichomonas vaginalis in genital specimens of female outpatients. Materials and Methods: Total number of subjects involved in the study was 130, among them 85 belonged to high-risk group and 45 belonged to low-risk group. Two high vaginal swabs were collected from each patient. Saline wet mount, Giemsa stain, and culture in modified cysteine peptone liver infusion maltose medium were performed. Results were tabulated and analyzed. Results: Saline wet mount was positive for trichomoniasis in seven individuals, Giemsa detected trichomoniasis in five patients, and culture was positive in eight patients. Of these eight culture positive cases, one was wet mount negative and four were Giemsa stain negative. Conclusion: Culture is more sensitive than wet mount and Giemsa stain.

APA, Harvard, Vancouver, ISO, and other styles

39

Kumalasari, Tri Novia. "Sensitivitas dan Spesifisitas Metode Brugia Rapid Test pada Pemeriksaan Brugia Malayi." Biomedical Journal of Indonesia: Jurnal Biomedik Fakultas Kedokteran Universitas Sriwijaya 5, no. 2 (May 31, 2019): 62–71. http://dx.doi.org/10.32539/bji.v5i1.7983.

Full text

Abstract:

Filariasis merupakan penyakit menular menahun yang disebabkan oleh cacing filaria yang menyerang saluran dan kelenjar getah bening. Diketahui 90% kasus filariasis penyebabnya adalah tiga spesies cacing filarial yaitu: Wuchereria bancrofti, Brugia malayi dan Brugia timori. Pemeriksaan secara mikroskopis pada sediaan darah tebal menggunakan darah tepi pasien pada malam hari merupakan teknik konvensional. Dalam program eliminasi filariasis global, WHO menganjurkan penggunaan metode serodiagnosis. Untuk filariasis Brugia, metode serodiagnosis terbaik yang ada saat ini adalah deteksi antibodi IgG4 anti-filaria.Tujuan Penelitian ini adalah untuk mengetahui sensitivitas dan spesifisitas metode Brugia Rapid Test dibandingkan dengan sediaan darah tepi dengan pewarnaan Giemsa dalam mendeteksi Brugia malayi di Desa Sungai Rengit Murni Kecamatan Talang Kelapa Kabupaten Banyuasin. Metode yang digunakan adalah uji Diagnostik. Penelitian ini dilakukan di Desa Sungai Rengit Murni Kabupaten Banyuasin pada tanggal 20 Juni 2013. Jumlah sampel 80 orang yang diambil secara Simple Random Sampling. Metode pemeriksaan dengan cara pewarnaan Giemsa dan metode Brugia Rapid. Berdasarkan hasil penelitian dari 80 sampel yang dilakukan pemeriksaan, pemeriksaan dengan metode Giemsa 0 (0%) dan dengan metode Brugia Rapid 22 Orang (27,5%) Penelitian ini menunjukkan bahwa metode Brugia rapid dibandingkan metode Giemsa Sensitivitasnya 0%, spesifisitasnya72,5%, nilai duga positif 0% dan nilai duga negatif 100%.

APA, Harvard, Vancouver, ISO, and other styles

40

Elias, Ruby Elizabeth, Bindiya Gisuthan, and Sreeganesh A.S. "Histopathological Changes in Helicobacter pylori Associated Gastritis and Scope of Special Stain and Immunohistochemistry as Diagnostic Aids." Journal of Evidence Based Medicine and Healthcare 7, no. 50 (December 14, 2020): 3027–32. http://dx.doi.org/10.18410/jebmh/2020/618.

Full text

Abstract:

BACKGROUND Helicobacter pylori associated chronic gastritis plays a vital role in the development of majority of gastric adenocarcinomas and most gastric MALT (Mucosa Associated Lymphoid Tissue) lymphomas. Many diagnostic methods are available for the identification of this organism. However, in gastroenterology practice, histopathological examination of biopsy samples provides visual identification of the pathogen and the associated mucosal changes with special stains like Giemsa. The aim of this study was to evaluate the efficacy of three stains H & E- (Haematoxylin and Eosin), Giemsa and IHC (Immunohistochemistry) in the identification of H. pylori. Associated histologic changes were noted and the relationship between the degree of colonisation and the activity and chronicity of gastritis were analysed. METHODS 585 gastric biopsies taken from dyspeptic patients were evaluated for gastritis, based on updated Sydney System. In 250 randomly selected cases, three staining methods were used. RESULTS Out of 585 cases, 413 (70.60 %) had features of chronic gastritis. Mild chronic gastritis was the commonest finding and is seen in most cases of mild H. pylori colonisation. When activity was monitored, mild activity was the most frequent finding [225 (38.46 %)]. Majority of the severe activity cases showed severe H. pylori colonisation. 13.16 %, 4.79 % and 7.35 % showed intestinal metaplasia, atrophy and dysplastic changes respectively. Out of 250 cases, H & E and Giemsa stains showed 45.6 % and 57.2 % positivity while IHC demonstrated maximum number of positivity (156 cases - 62.4 %). Sensitivity and specificity of H & E was found to be 77.90 % and 98.95 %, positive predictive value was 99.13 % and negative predictive value was 69.18 %. For Giemsa stain, sensitivity was 91.67 %, specificity was 100 %, positive predictive value was 100 % and negative predictive value was 87.85 %. DISCUSSION H. pylori gastritis was a frequent finding in dyspeptic patients in southern part of India. When chi-square test was done, a significant statistical relationship between the severity of H. pylori colonisation, activity and chronicity of gastritis was noted. P value was < 0.001. With the use of special stain, Giemsa and ancillary techniques like IHC, the detection rate of H. pylori was enhanced considerably. CONCLUSIONS With increasing number of H. pylori in the mucosa, there was increase in the chronicity and activity of gastritis. Although immunohistochemistry revealed more cases of H. pylori, Giemsa can be a cost-effective substitute, because of its high specificity and positive predictive value. KEYWORDS H. pylori Gastritis, Giemsa, Haematoxylin and Eosin Stain, Immunohistochemistry

APA, Harvard, Vancouver, ISO, and other styles

41

Bradbeer, J. N., M. Riminucci, and P. Bianco. "Giemsa as a fluorescent stain for mineralized bone." Journal of Histochemistry & Cytochemistry 42, no. 5 (May 1994): 677–80. http://dx.doi.org/10.1177/42.5.7512588.

Full text

Abstract:

We present evidence for a previously unrecognized differential staining effect of Giemsa solution in fluorescence microscopy. The effect consists of selective fluorescent staining of mineralized bone (and elastic fibers) in tissue sections and, like the classical Romanowsky effect, is based on the differential binding of Eosin Y to tissue structures in the presence of Azur II and Methylene Blue. This effect opens the way to new applications of the Giemsa solution in fluorescence microscopy and in confocal fluorescence microscopy.

APA, Harvard, Vancouver, ISO, and other styles

42

Yuniastutik, Titin. "Penentuan Konsentrasi Pewarna Giemsa, Waktu Dan Suhu Inkubasi Pada Aktifitas Fagositosis Ikan Lele (Clarias Sp.) Yang Diinfeksi Bakteri Aeromomonas hydrophila dan Vibrio harveyi." Jurnal Temapela 2, no. 1 (July 17, 2019): 52–58. http://dx.doi.org/10.25077/temapela.2.1.52-58.2019.

Full text

Abstract:

Pektikum hematologi darah terutama uji fagositosis tentang konsentrasi giemsa yang digunakan, waktu inkubasi sampel darah dan suhu inkubasi sampel darah yang masih menggunakan perkiraan. Hal tersebut menyebabkan hasil pemeriksaan menjadi tidak akurat, tidak optimal dan kurang dapat dipercaya untuk menjawab pertanyaan riset dan mempersulit didalam menegakkan diagnosa suatu penyakit ikan. Penggunaan prosedur baku tidak menjamin akan diperolehnya hasil yang baik, sehingga diperlukan pemilihan terhadap durasi yang tepat pada proses pewarnaan menggunakan pewarna giemsa. Ketelitian dan ketepatan sangat diperlukan dalam setiap tahap pembuatan sediaan preparat apusan darah. Penelitian ini bertujuan untuk menentukan konsentrasi pewarna giemsa yang digunakan, waktu inkubasi sampel darah, dan suhu inkubasi sampel darah yang optimal dalam metode aktifitas fagositosis. Metode yang digunakan dalam penelitian ini adalah metode experimen, dengan menerapkan variasi konsentrasi giemsa, waktu inkubasi, dan suhu inkubasi pada proses pembuatan sampel darah untuk fagositosis, kemudian dilakukan proses pengamatan sel darah ikan dan masing masing hasil pengamatan dibandingkan untuk mengetahui hasil yang terbaik. Pengambilan data dilakukan dengan cara observasi dan dokumentasi dari pekerjaan rutin penulis di Laboratorium Budidaya Ikan Divisi Penyakit Dan Kesehatan Ikan. Berdasarkan hasil dari penelitian yang divisualisasikan melalui pengamatan mikroskop, maka dapat disimpulkan bahwa pada metode aktifitas fagositosis konsentrasi giemsa yang digunakan adalah 100%, waktu inkubasi sampel darah terbaik adalah 40 menit, dan suhurmasalahan yang sering terjadi di Laboratorium Budidaya Ikan Divisi Penyakit Dan Kesehatan Ikan adalah pra inkubasi sampel darah terbaik adalah 30oC dan secara signifikan berpengaruh terhadap kualitas gambaran sel darah ikan. Hal tersebut sangat bermanfaat untuk menunjang bahan pengajaran dan praktikum mahasiswa, membantu para peneliti untuk menjawab permasalahan yang timbul serta sangat diperlukan dalam menunjang diagnosa ikan.

APA, Harvard, Vancouver, ISO, and other styles

43

Rahadiyanti, Dyatiara Devy, Evy Ervianti, Damayanti Damayanti, Dwi Murtiastutik, Sawitri Sawitri, and Afif Nurul Hidayati. "The Concordance of Three Diagnostic Test for Malassezia folliculitis using Potassium Hydroxide 20% + Blue-Black Parker Ink, May Grunwald Giemsa, and Potassium Hydroxide 10% + Chicago Sky Blue." Berkala Ilmu Kesehatan Kulit dan Kelamin 32, no. 1 (March 31, 2020): 33. http://dx.doi.org/10.20473/bikk.v32.1.2020.33-39.

Full text

Abstract:

Background: Malassezia folliculitis is a pilosebaceous follicular infection disease caused by Malassezia species. There are many misdiagnosed Malassezia folliculitis cases, causing the maladministration of therapy. A routine diagnostic test performed for Malassezia folliculitis cases is the identification of fungal elements (spore) with a microscope using potassium hydroxide, but it has several weaknesses. Purpose: To evaluate the suitability of Malassezia folliculitis diagnostic test using Potassium Hydroxide 20% + Blue-Black Parker Ink, May Grunwald Giemsa, and Potassium Hydroxide 10% + Chicago Sky Blue. Methods: Analytic observational study conducted in the Dermatomycology Division of Dermatology and Venereology outpatient clinic, Dr. Soetomo General Hospital Surabaya. The samples were thirty patients with clinical features of Malassezia folliculitis. The research material was obtained from the body as many as three pieces of papulomoluscoid lesion extracted. The material obtained was then divided into three glass objects for Potassium Hydroxide 20% + Blue-Black Parker Ink, May Grunwald Giemsa, and Potassium Hydroxide 10% + Chicago Sky Blue staining. Result: The identification of spores using Potassium Hydroxide 20% + Blue-Black Parker Ink was 90%, May Grunwald Giemsa was 90%, and Potassium Hydroxide 10% + Chicago Sky Blue was 93% with a value of κ=0.348 and p=0.051. The diagnostic values of May Grunwald Giemsa and Potassium Hydroxide 10% + Chicago Sky Blue were 96.6% sensitivity, 33.3% specificity, 92.9% Positive Predictive Value, and 50 % Negative Predictive Value. Conclusions: There was no significant concordance between May Grunwald Giemsa and Potassium Hydroxide 10% + Chicago Sky Blue with Potassium Hydroxide 20% + Blue-Black Parker Ink in establishing the diagnosis of Malassezia folliculitis. Potassium Hydroxide 20% + Blue-Black Parker Ink is still needed as a routine examination in cases with clinical features of Malassezia folliculitis.

APA, Harvard, Vancouver, ISO, and other styles

44

Dolan, M. "The role of the Giemsa stain in cytogenetics." Biotechnic & Histochemistry 86, no. 2 (March 14, 2011): 94–97. http://dx.doi.org/10.3109/10520295.2010.515493.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Bottone, Edward J., and Gary P. Wormser. "Cryptococcus neoformans: Giemsa Stained Characteristics That Facilitate Detection." Laboratory Medicine 23, no. 2 (February 1, 1992): 120–21. http://dx.doi.org/10.1093/labmed/23.2.120.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Armstrong, K. C., Iris L. Craig, and C. Merritt. "Hordeum chilense (2n = 14) computer-assisted Giemsa karyotypes." Genome 29, no. 5 (October 1, 1987): 683–88. http://dx.doi.org/10.1139/g87-117.

Full text

Abstract:

The Giemsa karyotype of three accessions of Hordeum chilense Roem. and Schult were studied in detail. The idiograms were developed using a computer-assisted software program that contained the ability to straighten and measure chromosomes. Distinctive banding patterns were formed on each of the seven chromosome pairs, which in conjunction with length and arm ratio allowed the identification of each chromosome. Some differences in the banding patterns of the accessions were noted. Key words: Hordeum, chromosome banding.

APA, Harvard, Vancouver, ISO, and other styles

47

Tsang, W. Y. W., J. K. C. Chan, and C. S. C. Wong. "Giemsa stain for histological diagnosis of bacillary angiomatosis." Histopathology 21, no. 3 (September 1992): 299. http://dx.doi.org/10.1111/j.1365-2559.1992.tb00398.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Churukian, Charles J. "Microwave Giemsa Technique for Paraffin Embedded Tissue Sections." Journal of Histotechnology 18, no. 4 (December 1995): 319–22. http://dx.doi.org/10.1179/his.1995.18.4.319.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

M�ller-Walz, R., and H. W. Zimmermann. "�ber Romanowsky-Farbstoffe und den Romanowsky-Giemsa-Effekt." Histochemistry 87, no. 2 (1987): 157–72. http://dx.doi.org/10.1007/bf00533401.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

D'EMERICO, SAVERIO, DOMENICO PIGNONE, and ANTONIO SCRUGLI. "Giemsa C-banded karyotypes in Serapias L. (Orchidaceae)." Botanical Journal of the Linnean Society 133, no. 4 (August 2000): 485–92. http://dx.doi.org/10.1111/j.1095-8339.2000.tb01591.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Giemsa'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles