Dissertations / Theses on the topic 'Glande salive'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Glande salive.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
RATOMPOSON, RATOMPONIONY GARY. "Tumeurs salivaires a manifestation parapharyngee : a propos de 13 observations cliniques." Aix-Marseille 2, 1990. http://www.theses.fr/1990AIX20128.
Full textRIAD, FOUAD. "Regulation endocrinienne de la secretion salivaire des mineraux chez les bovins." Clermont-Ferrand 2, 1986. http://www.theses.fr/1986CLF21026.
Full textLarsson, Olof. "Peptides as cotransmitters in salivary secretion histochemical, biochemical and functional studies of parotid and submandibular glands /." Stockholm : Kongl. Carolinska Medico Chirurgiska Institutet, 1989. http://catalog.hathitrust.org/api/volumes/oclc/19412146.html.
Full textYuk-lun, Kam. "The efficacy of a novel lubricating system in the management of radiotherapy related xerostomia." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31981835.
Full textLeslie, Martin David. "Salivary gland function after radiotherapy for head and neck cancer." Thesis, University College London (University of London), 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341706.
Full textEliasson, Lars. "On minor salivary gland secretion /." Göteborg : Department of Cariology, Institute of Odontology, Sahlgrenska Academy at Göteborg University, 2006. http://hdl.handle.net/2077/712.
Full textSanderson, Christopher Mark. "Transport of IgA in rat salivary glands." Thesis, University of Surrey, 1986. http://epubs.surrey.ac.uk/847984/.
Full textLaplume, Sylvie. "Analyse biologique de la salive et applications dans les domaines cliniques." Paris 5, 1996. http://www.theses.fr/1996PA05P075.
Full textMason, Gillian Ivy. "A study of salivary glands and saliva in health and disease." Thesis, University of Birmingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368520.
Full textDantas, Aline Maia. "Estudo da relação entre glândulas salivares e doença periodontal em ratos." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/42/42136/tde-09022012-142657/.
Full textGingivitis and periodontitis are common infectious periodontal diseases in man, in which periodontal bacteria and their products participate actively to induce local inflammation and systemic effects (eg heart). Knowing that saliva represents the first major barrier to oral infections, this study aimed to: i) to assess bone loss due to induced periodontitis in rats, after intervals of 3, 7 and 14 days, ii) to investigate possible changes in flow (or not stimulated with pilocarpine) and salivary composition in these animals, and iii) evaluate the concentration and expression of markers of oxidative stress and inflammation in the salivary glands and saliva samples, and iv) assess the functional role of salivary gland function in ex vivo production of amylase. For this purpose, male Wistar rats (180-200g) underwent induction of periodontitis by implanting the ligature, and biochemical and inflammatory parameters were assessed. Rats with periodontitis on day 3 when compared to sham group, exhibited a significant increase in salivary flow (stimulated with pilocarpine), production of Ca2+, protein secretion and production of amylase in saliva, as well as increased contents of TBARS in the parotid and amylase released from submandibular glands (GSM). There was also increased expression of mRNA for iNOS and nNOS in GSM. In contrast, rats with periodontitis after 7 days exhibited a reduction in stimulated saliva (not stimulated), production and total protein concentration and secretion of salivary amylase, although the content of serum and salivary TBARS and the activity of MPO in saliva of these animals was high compared to the sham group. There were no significant differences in TBARS content in salivary gland secretion and Ca2+ concentration in saliva, nor on the content of nitrated proteins in samples from these animals GSM. By the 14th day envisioned an increase of NOS activity and Ca2+ dependent mRNA expression of iNOS and nNOS in GSM. Rats with periodontitis, 14 days after induction, exhibited a significant increase in the rate of salivation, concentration and salivary secretion of Ca2+, production and concentration of total protein, salivary amylase and content of TBARS in samples of saliva and salivary glands. Still, there was increased activity of peroxidase / MPO, nitrate concentration in saliva and proteins nitrated in GSM and higher concentration of Th1 / Th2 (IL-4, IL-13 and IL-10) in samples of GSM. We conclude that the experimental induction of periodontal disease in rats, influence the functioning of the salivary glands according to the days of induction, initially stimulating, in a second time and subsequently inhibiting return to baseline levels. After 7 days, is characterized as the ideal time for the expression of an inhibitory effect on the gland function.
Sardinha, Daniela Abreu. "Xerostomia e hipofunção das glândulas salivares: fisiopatologia e enquadramento clínico." Master's thesis, [s.n.], 2013. http://hdl.handle.net/10284/5250.
Full textA saliva desempenha múltiplas funções e tem um papel vital na proteção da saúde dos tecidos moles e duros da cavidade oral. A xerostomia é a sensação subjetiva de boca seca, um sintoma que pode ou não estar acompanhado de hipossalivação, descrita como redução do fluxo salivar estimulado ou não estimulado. Os pacientes com redução do fluxo salivar apresentam maior risco de cáries, infeções fúngicas orais, dificuldades na deglutição e alteração do paladar. As principais causas da hipofunção das glândulas salivares e da xerostomia são doenças sistémicas e os medicamentos. O diagnóstico é baseado em métodos simples, como a medição do fluxo salivar estimulado e não estimulado. Sendo que o tratamento da xerostomia é essencialmente realizado tendo em conta a sua causa e é dividido em quatro categorias: prevenção, tratamento sintomático, estimulação local ou tópica e terapias sistémicas ou estimulação sistémica. Cada categoria será aplicada dependendo da função glandular. Quando há alguma preservação da estrutura da GS os estimulantes salivares (estimulação local ou sistémica) são o mais indicado. Quando as GS encontram-se irreversivelmente danificadas a opção é o tratamento paliativo.
Saliva has multiple functions and plays a vital role in protecting the health of hard and soft tissues of the oral cavity. Xerostomia is the subjective sensation of dry mouth, a symptom that may or may not be accompanied by hyposalivation, described as a reduction of unstimulated and stimulated salivary flow. Patients with reduced salivary flow are at increased risk for caries, oral fungal infections, swallowing problems and altered taste. The principal causes of salivary gland hypofunction and xerostomia are systemic diseases and drugs. The diagnosis is based on simple methods, as measuring both unstimulated and stimulated salivary flow rate. Treatment of xerostomia essentially is carried out in regard to the cause and is divided in four main categories: palliative or symptomatic stimulation, local and systemic stimulation and prevention of complications. Which category will be applied, depending on salivary glands function. In cases when there is still some residual salivary function it was shown that saliva stimulans (local or systemic stimulation) is more appropriate. When salivary glands are irreversible damaged palliative treatment is the best option.
Funegård, Ulrika. "Fractionated irradiation of salivary glands : loss and protection of function." Doctoral thesis, Umeå universitet, Kardiologi, 1995. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-100594.
Full textS. 1-82: sammanfattning, s. 85-152: 6 uppsatser
digitalisering@umu
Aksamit, Matthew Stephen. "Bioinformatic analysis of pea aphid salivary gland transcripts." Thesis, Kansas State University, 2014. http://hdl.handle.net/2097/32836.
Full textBiochemistry and Molecular Biophysics Interdepartmental Program
Gerald Reeck
Pea aphids (Acyrthosiphon pisum) are sap-sucking insects that feed on the phloem sap of some plants of the family Fabaceae (legumes). Aphids feed on host plants by inserting their stylets between plant cells to feed from phloem sap in sieve elements. Their feeding is of major agronomical importance, as aphids cause hundreds of millions of dollars in crop damage worldwide, annually. Salivary gland transcripts from plant-fed and diet-fed pea aphids were studied by RNASeq to analyze their expression. Most transcripts had higher expression in plant-fed pea aphids, likely due to the need for saliva protein in the aphid/plant interaction. Numerous salivary gland transcripts and saliva proteins have been identified in aphids, including a glutathione peroxidase. Glutathione peroxidases are a group of enzymes with the purpose of protecting organisms from oxidative damage. Here, I present a bioinformatic analysis of pea aphid expressed sequence tag libraries that identified four unique glutathione peroxidases in pea aphids. One glutathione peroxidase, ApGPx1 has high expression in the pea aphid salivary gland. Two glutathione peroxidase genes are present in the current annotation of the pea aphid genome. My work indicates that the two genes need to be revised.
Kam, Yuk-lun, and 甘玉麟. "The efficacy of a novel lubricating system in the management of radiotherapy related xerostomia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31981835.
Full textZenteno, Savin Tania. "Plasma arginine vasotocin and angiotensin II concentrations during saline acclimation in birds with salt glands." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/29892.
Full textScience, Faculty of
Zoology, Department of
Graduate
Lepesqueur, Laura Soares Souto. "Efeitos da fibrose cística sobre o microbioma bucal e o proteoma salivar /." São José dos Campos, 2019. http://hdl.handle.net/11449/183394.
Full textCoorientadora: Marcia Hiromi Tanaka
Banca: Luana Marotta Reis de Vasconcellos
Banca: Bruno Mello de Matos
Banca: Soraya Carvalho da Costa
Banca: Daniel Freitas Alves Pereira
Resumo: A Fibrose Cística (FC) é uma doença genética de elevada prevalência global e que causa função anormal das glândulas exócrinas. As alterações nas funções das glândulas salivares podem impactar a saúde bucal que por sua vez podem influenciar a saúde geral. A boca pode representar um reservatório microbiano de potenciais patógenos e colonizadores das vias aéreas, causando infecções crônicas pulmonares. O objetivo deste estudo foi avaliar os impactos da FC na cavidade bucal, saliva e microbioma bucal. Foram incluídos no estudo 50 pacientes com diagnóstico de FC com idades de 3 a 20 anos, divididos em 2 grupos de acordo com o grau de severidade da doença determinado pelo escore de Shwachman-Kulczycki: G1 (baixa severidade) e G2 (alta severidade). Foi também incluído grupo controle pareado ao grupo de estudo quanto ao gênero e idade (G3, n=50). A presença de lesões de cárie foi avaliada. O impacto da FC sobre a saúde bucal foi avaliado por questionário preenchido pelos pais ou responsáveis. Amostra de saliva estimulada foi coletada de todos os pacientes. O microbioma bucal foi avaliado por Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) e metodologias de cultivo, para análise da microbiota potencialmente oportunista e cariogênica. Realizou-se ainda a análise proteômica da saliva e quantificação de imunoglobulinas salivares. Os resultados foram analisados e, de acordo com a distribuição dos dados e avaliação desejada, foram aplicados os testes estatístic... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract : Cystic Fibrosis (CF) is a genetic disease with high global prevalence that causes abnormal function of the exocrine glands. The functional alterations of salivary glands and saliva can impact the oral health and influence general health. Oral cavity may represent a microbial reservoir of potential pathogens that can colonize the airways and cause chronic pulmonary infections. The aim of this study is to evaluate the impact of cystic fibrosis on the oral cavity, saliva and oral microbiome. Fifty CF patients aged from 3 to 20 years were divided into two groups according to the disease severity determined by the Shwachman-Kulczycki score: G1 (low severity) and G2 (high severity). Also, age and gender paired control group was included in the study (G3, n = 50). The occurrence of caries was evaluated. The impact of CF on oral health was evaluated by a questionnaire filled by parents or responsible person. Stimulated whole saliva (WS) samples were collected from all patients. The oral microbiome was analyzed by Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) and by microbiological culture methodologies to evaluate the potential opportunistic and cariogenic microbiota. The proteomic analysis of saliva and quantification of salivary immunoglobulins were carried out. Statistical analysis was performed according to the normality of the data at a significance level of 5%. The applied questionnaire pointed out that oral health did not impact systemic health negatively, according to the parents in all groups. The groups of patients with CF had lower rates of dmft, DMFT, salivary flow rate and initial pH in comparison to the control group. The counts of staphylococcal and yeast from CF groups were significant higher than the controls. All fungal isolates were susceptible to the antifungal agents. Higher incidence of bacterial resistance was ... (Complete abstract click electronic access below)
Doutor
Trinca, Vitor. "Análise do padrão de expressão de BhC4-1-GFP em linhagens transgênicas de Drosophila melanogaster." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/17/17136/tde-19072018-104348/.
Full textOur laboratory investigates the molecular mechanisms that promote the establishment of developmentally regulated gene expression patterns in metazoans. As a model, we employ the BhC4-1 DNA puff gene, which is amplified and expressed in a regulated manner in the salivary gland and in the prothoracic gland at the end of the fourth larval instar in B. hygida. Functional studies in D. melanogaster resulted in the identification of cis-regulatory modules (CRMs) in the BhC4-1 promoter region. The 67 bp (-253/-187) ring gland CRM drives BhC4-1-lacZ expression in the ring gland from late embryonic development. The 129 bp (-186/-58) CRM salivary gland drives transgene expression in the prepupal salivary glands. The ring gland is the major endocrine organ, and comprises the prothoracic glands (synthesis of ecdysteroid hormones), corpus allatum (synthesis of juvenile hormone) and corpus cardiacum (neuroendocrine gland). In this work, 12 independent lines transformed with a construct containing the BhC4-1 promoter fragment (- 253/+40) cloned upstream of the reporter gene GFP were obtained. The genotype of each line was validated using Southern blots. Initially, the 12 obtained lines were analyzed to investigate the pattern of GFP expression in the third instar larvae and in the 2 hours prepupae. This initial screening revealed that the pattern of GFP expression is highly variable in these lines. The developmental pattern of GFP expression was extended in two representative (- 253/+40)/GFP lines. In these lines, GFP expression is initially detected in the larval and prepupal salivary glands and in ring gland third instar. Differently from previously observed in (-253/+40)/lacZ lines, in these lines we did not detect GFP expression at developmental times prior to the third larval instar. Gene interaction experiments revealed that in the absence of the br transcription factor, GFP expression is maintained in the ring gland and abolished in the salivary gland of third instar larvae. The results of gene interaction experiments corroborate previous data indicating the set of transcription factors that regulate BhC4-1-lacZ expression in the ring gland is distinct from that which promotes gene expression in salivary glands. The lines obtained in this work constitute a tool to characterize the tissue-specific transcription factors that regulate BhC4-1 gene in the ring gland and/or in the salivary gland from the end of the larval development.
Abd, El-All M. A. H. "Comparative histological, histochemical, ultrastructural, scanning electron microscopic and functional studies of the integumental glands ...of A. Salina." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381091.
Full textLeite, Mariana Ferreira. ""Estudo temporal do efeito da administração de tungstato de sódio sobre alguns parâmetros de glândulas salivares e saliva de ratas diabéticas"." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/23/23140/tde-28082006-183554/.
Full textThe aim of study was evaluate the effect of sodium tungstate administration (2mg/ml) on some parameters of parotid, submandibular and pilocarpine/isoproterenol stimulated saliva of streptozotocin induced-diabetes, during six weeks. The groups were divided in untreated control (C), treated control (CT), untreated diabetic (D) and treated diabetic (DT). The parameters studied were energetic metabolism, proteic composition of glands and saliva, besides the protein secretion pathway. In the energetic metabolism were determinated hexokinase, phosphofructokinase-1, pyruvate kinase, glycose-6-phosphate dehydrogenase and lactate dehydrogenase activities. Were evaluated the total protein concentration, amylase and peroxidase activities and free and total sialic acid content on saliva and salivary glands. The protein secretion pathway was studied by evaluation of expression of protein kinase C by western blot. The results obtained confirm the tungstate potential as hypoglycemic, as well as its action in the polifagia, polidipsia and body and glandular weight control. The glycogen concentration suffered an increment in the diabetic group and the tungstate treatment potentialized the increase in the salivary glands. Parotid glands suffered an increased in some parameters of glycolitic pathway and its protein composition in the initial weeks of study, with normalization in the end of experiment. The tungstate was effective in the control of peroxidase activity in salivary glands, but few effects on the other parameters. Diabetic animals presented an increased of total protein concentration in saliva, but no difference was observed in the amylase and peroxidase activities. Sodium tungstate caused an increased in the total protein concentration and a reduction on amylase activity of saliva in the CT and DT groups. Submandibular gland of diabetic rats suffered stimulation in the active and inactive PKC expression after one week and alteration in the isoforms profile of enzyme. Sodium tungstate potencialized this increased. Conclusion: In this study, was not observed effect of sodium tungstate on energetic metabolism of parotid and submandibular, showing that this compound does not alter the metabolism in the peripheral tissue as salivary glands. The tungstate acts on peroxidase activity, this show the possible action of this compound in the antioxidant system. Sodium tungstate stimulates the protein secretion pathway in the salivary glands.
Bizzarro, Bruna. "Efeito da saliva de Aedes aegypti sobre a diferenciação, maturação e função de células dendríticas e na proliferação de linfócitos T." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-21092012-082114/.
Full textMosquitoes are the most important vectors of human pathogens, transmitting a wide range of emerging and re-emerging infectious diseases. In this scenario, Aedes aegypti is a relevant mosquito species. In the present study, we have investigated the activities of the salivary gland extract (SGE) of this mosquito vector on the dendritic cell and T lymphocyte biology. Our data reveals that the SGE does not interfere on the differentiation, maturation and function of murine dendritic cells. However, salivary components of these mosquitoes have a direct effect on lymphocytes. The mechanism of action of SGE involved apoptosis of naïve T cells, while memory cells were more resistant to this activity. A molecule with molecular weight above 400 kDa is likely responsible for this effect. .Taken together, the results generated by this work contribute to the understanding of the biological effects of Ae. aegypti saliva on the host and, consequently, its role on the transmission of diseases.
Bhattacharya, Sumit. "Contribution of Purinergic Receptors to Calcium Signaling in Salivary Gland." University of Toledo Health Science Campus / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=mco1353370433.
Full textUEDA, MINORU, TOSHIO SHlGETOMI, TSUNETOSHI HAYASHI, and HIDEAKI KAGAMI. "ASSESSMENT OF THE EFFECTS OF AGING AND MEDICATION ON SALIVARY GLAND FUNCTION IN PATIENTS WITH XEROSTOMIA USING 99mTC-SCINTIGRAPHY." Nagoya University School of Medicine, 1995. http://hdl.handle.net/2237/16091.
Full textCampos, Amândio José Nova. "Estudo clínico em pacientes medicados com inibidores selectivos da recaptação da serotonina e noradrenalida: avaliação da função salivar e repercussão a nível oral." Bachelor's thesis, [s.n], 2008. http://hdl.handle.net/10284/1966.
Full textA saliva desempenha múltiplas funções e tem um papel vital na protecção da saúde dos tecidos moles e duros da cavidade oral. As reduções no fluxo salivar manifestam-se mais frequentemente como sintomas de boca ressequida sendo esta queixa subjectiva denominada xerostomia. Embora a xerostomia seja o indicativo mais frequente de redução da produção salivar, não está invariavelmente associado com hipossalivação. A depressão é a mais comum das doenças mentais estando os antidepressores na primeira linha do tratamento da maioria dos doentes com depressão e a esta classe terapêutica estão, inevitavelmente associados efeitos secundários e reacções adversas sendo a xerostomia um sintoma que parece ser transversal a todos eles. Este estudo epidemiológico, observacional, transversal, analítico tipo caso-controlo, retrospectivo, tem como objectivo avaliar o possível impacto indirecto dos inibidores selectivos da recaptação da serotonina e noradrenalina (ISRS/ISRSN) na integridade dentária e na mucosa oral, tendo em conta o fluxo salivar em repouso e estimulado, relacionado com o tempo de toma, o pH salivar e o diagnóstico de xeroftalmia, tendo sido inspeccionada, detalhadamente, a mucosa oral. A amostra em estudo contou com 60 pacientes os quais foram divididos em dois grupos: 30 medicados com inibidores selectivos da recaptação da serotonina e noradrenalina e os outros 30 não medicados e sem sintomas. Conclui-se que: o tempo de toma de ISRS/ISRSN influencia, de forma inversa, o FSE, isto é, com a duração do tempo de toma, o FSE tende a diminuir e de forma directa, a xeroftalmia, isto é, quanto maior a duração do tempo de toma, maior a sensação subjectiva de boca seca.--- Saliva has multiple functions and plays a vital role in protecting the health of hard and soft tissues in the oral cavity. Reductions in salivary flow manifest themselves more often as symptoms of dry mouth being subjective complaint called xerostomia. Although xerostomia is the most frequent indication of reduced saliva production, is not invariably associated with the licensee hipossalivation. The depression is the most common mental disorders and antidepressants are in the first line of treatment for most patients with depression and this therapeutic class is, inevitably, associated side effects and adverse reactions and xerostomia is a symptom that seems to cross all of them. This study, epidemiological, observational, cross-analytical type case-control, retrospective, aims to assess the possible impact of selective reuptake inhibitors of serotonin and norepinephrine (SSRI/SNSRI) in dentistry and integrity in oral tissues, taking into account salivary flow at rest and stimulated by linking with the time of taking the salivary pH and diagnosis of xerostomia, and was inspected in detail, the oral tissues. The sample under study had 60 patients who were divided into two groups: 30 medicated with selective reuptake inhibitors of serotonin and norepinephrine and the other 30 non-medicated and without symptoms. It was concluded that: the time of taking SSRI/SNSRI influence, in reverse, the ESF, that is, with the length of time that takes, the ESF tends to decline and in a direct manner, xerostomia, that is, as the longer the duration of time that takes, the more subjective sensation of dry mouth.
Leinonen, J. (Jukka). "Carbonic anhydrase isoenzyme VI: distribution, catalytic properties and biological significance." Doctoral thesis, University of Oulu, 2008. http://urn.fi/urn:isbn:9789514289903.
Full textJurysta, Cédric. "Etude du flux salivaire de glucose et des transporteurs de l'hexose exprimés dans les parotides de sujets sains et diabétiques." Doctoral thesis, Universite Libre de Bruxelles, 2016. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/228910.
Full textDoctorat en Sciences dentaires
info:eu-repo/semantics/nonPublished
Carvalho, Polliane Morais de 1981. "Evaluation of masticatory, salivary and anthropometric function, presence of volatile sulphur compounds in young adults and changes in salivary gland pos adipogenesis induction in animal model = Avaliação da função mastigatória, salivar, antropométrica, presença de compostos sulfurados voláteis em adultos jovens e alterações em glândula salivar após indução de adipogênese em modelo animal." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/287939.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2018-08-26T13:36:25Z (GMT). No. of bitstreams: 1 Carvalho_PollianeMoraisde_D.pdf: 2230005 bytes, checksum: a03e416e657adde52ad89b778975278c (MD5) Previous issue date: 2014
Resumo: Este estudo investigou a função mastigatória, o paladar, a presença de compostos sulfurados voláteis (CSV) e a bioquímica salivar em sujeitos saudáveis (18-33 anos), e a indução de adipócitos em glândula salivar e suas implicações em modelo animal. Três estudos foram conduzidos, apresentados na forma de capítulos. Capítulo 1: Investigou a performance e habilidade mastigatória, paladar e a possível relação com gênero, índice de massa corporal (IMC), circunferência de cintura (CC) e fluxo salivar estimulado (Stim) e não estimulado (Unst). Foram avaliados 171 indivíduos, (125? 46?). A performance mastigatória foi determinada pela capacidade de fragmentação do Optocal plus e habilidade mastigatória com o uso da escala visual analógica (EVA). O paladar foi verificado pela percepção dos quatro sabores primários. A estatística descritiva, teste de normalidade, correlação, comparação e modelos de regressão foram utilizados, sendo as variáveis dependentes a função mastigatória e o paladar e as independentes: gênero, idade, variáveis antropométricas e fluxo salivar. Na análise de regressão linear múltipla, as variáveis independentes não predisseram o modelo para performance mastigatória. Com a habilidade mastigatória (HM) o modelo explicou 14% da variabilidade e para o paladar 5%. Os resultados indicaram que a performance não foi relacionada com parâmetros antropométricos e salivares em indivíduos jovens saudáveis. A habilidade foi relacionada com IMC, CC e gênero. O paladar foi fracamente relacionado ao IMC e CC. Capítulo 2: Verificou a bioquímica salivar e presença de CSV e a possível interferência do IMC, fluxo e pH stim/unst. Para a verificação dos CSV foram avaliados 71 voluntários (57? 14 ?), utilizando o aparelho Oral chromeTM. Foram determinadas as concentrações de proteína total, cálcio, fosfato, amilase e ureia em 171 voluntários (125? 46 ?), em saliva stim/unst. A bioquímica salivar foi semelhante em relação à antropometria. No entanto, as respectivas concentrações diferiram significativamente entre saliva Stim/Unst, com exceção da amilase. Os indivíduos apresentaram quantidades semelhantes de CSV em relação ao IMC. Em indivíduos com valores críticos de metilmercaptana (CH3SH) observou-se correlação significativa (r=0.51) com o pH (unst). Capítulo 3: Investigou o eventual aparecimento de adipócitos nas glândulas salivares em modelo animal (camundongos). A adipogênese foi realizada com dieta rica em gordura, e ainda via receptor de proliferadores de peroxissoma gamma (PPAR gama) com rosiglitazona. Western blot, histoquímica e imunoistoquímica foram utilizadas. Análise de microarray foi realizada para verificar o efeito da dieta. Anticorpos: fosfo-4E BP1 e tirosina hidroxilase marcaram a atividade de mTOR e nervos, respectivamente. O microarray mostrou um número significativo de alterações genéticas. Em relação à dieta observou-se baixa ou nenhuma expressão de fosfo 4E-BP1 e aumento na atividade de tirosina hidroxilase. Em camundongos tratados com rosiglitazona verificou-se ativação de mTOR e tirosina hidroxilase. Conclusão: Pelos resultados dos três capítulos concluiu-se que em indivíduos jovens e saudáveis a função mastigatória e paladar não foram influenciados pelo padrão salivar e foram fracamente relacionados ao antropométrico. A bioquímica salivar e presença de CSV foi semelhante em relação à antropometria. Observaram-se mudanças relacionadas à atividade do sistema nervoso em glândula salivar de camundongos devido à dieta rica em gordura ou ativação de PPAR gamma
Abstract: This study investigated masticatory function, the presence of volatile sulfur compounds (VSC) and salivary biochemistry in healthy subjects (18-33 years), and also the induction of adipocytes in the salivary gland and its implication in an animal model. Three studies were conducted, presented as chapters. Chapter 1: Investigated the performance and chewing ability, taste, and the possible relationship to gender, body mass index (BMI), waist circumference (WC) and stimulated salivary flow (Stim) and unstimulated (Unst). 171 individuals (125? 46?) were evaluated. Masticatory performance was determined by the ability of fragmentation Optocal plus and chewing ability with the use of visual analogue scale (VAS). Taste was verified by the perception of the four primary flavors. Descriptive statistics, normality tests, correlation, comparison and multiple linear regression models were used. In multiple linear regression performance was not predict by the independent variables in the model. With chewing ability the model explained 14% of variability and 5% for the taste. The results indicated that masticatory performance was not related to anthropometric parameters and saliva in healthy young subjects. The ability was related to BMI, WC and Gender. Taste was weakly related to BMI and WC. Chapter 2: Verify the salivary biochemistry and presence of VSC and the possible influence of BMI, flow and pH (Stim)/(Unst). For the verification of VSC 71 volunteers (14 57? ?) were assessed using the Oral chromeTM device. The concentrations of: total protein, calcium, phosphate, urea and amylase were investigated in 171 volunteers (46 125? ?) in saliva (Stim) / (Unst). Biochemical salivary were similar in respect of anthropometry. However, the concentrations differed significantly between saliva (Stim)/(Unst), with the exception of amylase. The sample presented similar amounts of CSV in relation to BMI. In individuals with critical values of methylmercaptan (CH3SH) we observed a significant correlation (r = 0:51) with pH Unst. The results indicate that in healthy young subjects salivary biochemistry and VSC exhibit similar behaviour in relation to BMI. The (CH3SH) when greater than the normal limit concentration was correlated to pH Unst. Chapter 3: We investigated the possible appearance of adipocytes in the salivary glands in animal model (mice). Adipogenesis was performed with high-fat diet, and also via peroxisome proliferator-gamma (PPAR gamma) with rosiglitazone . Western blot, histochemistry and immunohistochemistry were used. Microarray analysis was performed to assess the effect of diet. Antibodies: phospho-4E-BP1 and tyrosine hydroxylase marked mTOR and nerves activity, respectively. The microarray showed a large number of genetic changes. Regarding diet was observed low or no expression of phospho-4E-BP1 and an increase in tyrosine hydroxylase activity. In mice treated with rosiglitazone there was activation of mTOR and tyrosine hydroxylase. The results suggest that there are changes in salivary gland innervation before stimuli for adipogenesis. Conclusion: We concluded that in healthy young individuals masticatory function was not influenced by the salivary pattern and was weakly related to anthropometric. Salivary Biochemical and presence of CSV was similar in relation to anthropometry. There are alterations in the activity of the nervous system in the salivary glands of mice due high fat diet or activation of PPAR gamma
Doutorado
Anatomia
Doutora em Biologia Buco-Dental
Paula, Fernanda de. "Mapeamento dos canais de água no processo de morfogênese das glândulas salivares humanas: estudo topográfico das aquaporinas 1,3 e 5." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/5/5133/tde-10082016-125353/.
Full textIntroduction: The human salivary glands morphogenesis depends on complex processes during the development period until they reach full structural maturity to perform its function - the synthesis and secretion of saliva. The saliva is a complex aqueous fluid considered essential to health and homeostasis of the oral cavity; its synthesis depends on several molecular mechanisms, including the transport of water, solutes, ions, amongst others across the cell membranes. The aquaporin family of proteins is essential in this process. This protein family consists of thirteen members that form channels across the cell membrane facilitating water and small solutes transportation, crucial to the regulation of quality and quantity of secreted saliva. Aims: In this scenario, we evaluated, using the immunohistochemistry technique, the expression pattern of aquaporins 1, 3 and 5 in the different phases of salivary glands development, in order to understand the role of these protein in the formation of human salivary gland morphogenesis. Methodology: 47 specimens of paraffin embedded human salivary glands at various developmental phases were included in the study. The specimens were derived from various sites of the oral cavity of 20 human fetuses aged between 14 and 25 weeks of gestation. All specimens were subjected to the imunohistochemical immunoperoxidase technique. The results were qualitatively and semiquantitatively analyzed according to the stage of glandular morphogenesis and express location of aquaporin. Results: In the bud stage, there was expression of aquaporin 1, 3 and 5 in all glandular epithelial cells; in pseudoglandular stage, the expression of these proteins was seen in rudimentary ducts (except aquaporin-1) and the terminal end buds (future acini); in the canalicular phase the aquaporins were mainly detected in the rudimentary ducts and acini. Finally, in terminal bud stage, the aquaporin 3 and 5 were detected in the membranes of the ducts and acinar cells expressed all aquaporins. Conclusion: This study showed the presence of aquaporins 1, 3 and 5 in human salivary glands during embryogenesis period. The topographic analysis of these proteins allowed us to identify differences in the expression pattern between the different structural regions and stages of glandular development, suggesting different roles for each of these proteins
Gonçalves, Liliana Maria do Vale Costa. "Avaliação do pH salivar em pacientes submetidos a radioterapia à região de cabeça e pescoço." Master's thesis, [s.n.], 2015. http://hdl.handle.net/10284/5245.
Full textA disfunção das glândulas salivares, como sequela da Radioterapia (RT) traduz-se geralmente em hipofunção salivar e consequente diminuição do pH. Este estudo avaliou o comportamento do pH salivar num grupo de 35 indivíduos submetidos a RT com fracionamento convencional, por tumores malignos da região de cabeça e pescoço. O acompanhamento foi obtido através de três avaliações a cada indivíduo. A primeira avaliação foi realizada antes do tratamento, a segunda após 15 sessões de tratamento e a última depois de terminado o tratamento. O pH salivar foi determinado através de indicadores de alaranjado de metilo e azul de bromotimol, colocados na face dorsal da língua. A média do valor de pH registada antes do tratamento foi 6,69. A medição intermédia demonstrou diminuição do valor de pH da saliva para 6.37, baixando para 6,31 até ao final do tratamento (p>0,05). Estes resultados demonstraram que a RT, no tratamento de tumores da cabeça e do pescoço, parece reduzir o pH salivar, sendo este valor dose-dependente.
The dysfunction of the salivary glands, as an effect of radiotherapy (RT), usually generates a salivary hypofunction and subsequent decrease of the pH value. This study aimed to evaluate the salivary pH behaviour in a group of 35 individuals under RT with conventional fractionation, for malignant tumours of the head and neck regions. The follow-up was obtained through three assessments to each individual. The first evaluation was conducted before treatment, the second one after 15 treatment sessions and the last one at the end of the treatment. The salivary pH was determined by methyl orange indicator and bromothymol blue indicator placed on the dorsal surface of the tongue. The average pH recorded before the treatment was 6.69. The intermediate measurement showed a diminution of the saliva pH value to 6:37, dropping to 6.31 by the end of treatment (p> 0.05). These results revealed that the RT for the treatment of tumours of the head and neck regions appears to reduce the salivary pH value, which is dose-dependent.
Junior, Walter Luiz Siqueira. ""Estudo de alguns parâmetros salivares em indivíduos com síndrome de DOWN"." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/23/23140/tde-13062005-115943/.
Full textThe aim of this study was to measure the flow rate, pH, buffer capacity, sialic acid, total protein concentrations, amylase and peroxidase activities and sodium, potassium, calcium, phosphorus, zinc and magnesium concentration whole saliva of individuals with Down syndrome aged 1 - 25 years. In individuals aged 1-5 years the whole saliva was collected under slight suction, while in the others individuals aged 6-10, 11-15, 15-20, 21-25 the whole saliva was collected with stimulation by chewing a piece of parafilm, for 10 minutes. The pH and the buffer capacity were determined using a digital pHmeter. The buffer capacity was measured by titration with 0.01 N HCl. Electrolyte concentrations were determined by inductively coupled argon plasma with atomic emission spectrometry. Sialic acid was determined by thiobarbituric acid assay. Protein was determined by the folins phenol reagent. Amylase was assayed measuring the maltose produced by the breakdown of starch and peroxidase with ortho dianisidine. For statistical analysis the date are presented as mean ± SD. Students t test was used to determine differences between the mean of the Down syndrome and control groups. No statistically significant differences were observed in sialic acid, phosphorus, zinc, magnesium and calcium concentration between the individuals with Down syndrome and control group. The sodium and total protein concentration and buffer capacity showed higher in the Down syndrome than in the control group. On the other hand the flow rate and potassium concentration, amylase and peroxidase activities were lower in the Down syndrome than in the control group. These results suggest that the Down syndrome persons present alteration in the metabolism of the duct and/or acinar cells of salivary glands.
Nogueira, Fernando Neves. ""Sistema antioxidante e peroxidação lipídica em glândulas salivares de ratos diabético"." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/23/23140/tde-17112004-181932/.
Full textThe aim of the present study is to determine the effect of diabetes in the activity of some contents from the antioxidant system of submandibular (SM) and parotid (P) salivary glands. The diabetic state was induced by an injection of streptozotocin (60mg/kg body weight) dissolved in 0,1M sodium citrate buffer pH 4,5. Forty-eight hours after the injection, blood was collected and the glycemia determined. Only those animals presenting a blood glucose above 350mg glucose/100ml blood was used in the study. Thirty days after the induction of the diabetes, the animals were sacrificed, the salivary glands were removed and blood was collected and used in the determinations. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) was determined in spectrofotometer. The content of reduced (GSH) and oxidized (GSSG) glutathione was determined in HPLC. The lipid peroxidation was determined in blood and in SM and P glands, measuring the tiobarbituric acid (TBARS) which reacts with malonaldeyde (MDA) from lipid peroxidation. The results show an increase in MDA concentration from SM and blood, but not on P gland. The content of GSH and GSSG from SM gland increase, and the content of GSH from P gland decreased. There is no difference in the activity of SOD in both salivary glands. The activity of CAT and GPx from P gland increase, but in SM gland there is no difference. We can conclude that the antioxidant system from the salivary glands have different responses in diabetes state after 30 days of the induction. In SM gland, there is no difference in the activity of the enzymes, but we have an increase in the MDA content. In P gland, we have an increase in the CAT and GPx activity, but not in MDA concentration.
Dsamou, Micheline. "Protéome salivaire et sensibilité à l'amertume chez l'Homme." Phd thesis, Université de Bourgogne, 2012. http://tel.archives-ouvertes.fr/tel-00935220.
Full textIbuki, Flávia Kazue. "Estudo do efeito da irradiação com laser de baixa intensidade no sistema antioxidante de glândulas salivares de ratas diabéticas induzidas por estreptozotocina." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/23/23140/tde-09112010-111500/.
Full textThe aim of the present study was to analize the effect of low-power laser irradiation in the antioxidant enzymatic system of submandibular (GSM) and parotid (GP) salivary glands of diabetic rats induced by streptozotocin. The rats were initially divided into non-diabetic animals (C) and diabetic-animals (D) and maintained by the experimental period of thirty days. Twenty-nine days after diabetes induction, the animals were randomly divided into six groups: three diabetic groups (D0, D5 and D20) and three non-diabetic groups (C0, C5 and C20), according with laser irradiation dose that each one received (0, 5 and 20J/cm2, respectively). For diabetes induction an intraperitoneal injection of streptozotocin (STZ) (60mg/Kg body weight), dissolved in 0.1M sodium citrate buffer, pH 4.5 was performed. In non-diabetic animals, only the citrate buffer was used. The diabetes condition was confirmed seventy-two hours after animals have received the STZ injection. Rats with blood glucose level higher than 14mM (250 mg/100ml) were considered diabetic. The laser irradiation was performed according to Simões et.al. method (2009). Twenty-four hours after the irradiation rats were euthanized. Then, immediately after the euthanasia, salivary glands were removed for biochemical analysis. The total antioxidant values (TAS) and the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxide (GPx) enzymes were determined in spectrophotometer. Analyzing the results we can conclude that in GSM of diabetic rats the laser irradiation with 20J/cm2 increased the SOD activity. With the two different doses, increased the TAS values and CAT activity. However, in GSM of non-diabetic rats, laser irradiation with 20J/cm2, decreased the TAS values and led to an increase in CAT activity, regardless of the dose. In parotid glands laser irradiation decreased the CAT activity with either dose of 5J/cm2 or 20 J/cm2 and the laser irradiation with dose of 5J/cm2, decreased the CAT activity in parotid glands of non-diabetic rats.
Lascane, Nelise Alexandre da Silva. "Avaliação do status das glândulas salivares parótida e submandibular na displasia ectodérmica hipoidrótica por meio da ultrassonografia." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/23/23141/tde-18082010-121333/.
Full textBackground- Hypohidrotic ectodermal dysplasia is a rare genetic disease, clinically characterized by defects involving skin and their adnexal structures, as well as oral structures such as teeth, and occasionally salivary glands. These latter manifestations include anodontia or hypodontia, conical teeth and xerostomia. Objective- To analyze possible alterations in major salivary glands of ten patients with ectodermal dysplasia, using image exam. Methods- Ultrasonography was performed in ten pediatric cases of hypohidrotic ectodermal dysplasia to investigate the status of parotid and submandibular salivary gland. Results- Three patients presented aplasia/hypoplasia of at least one gland examined. The other 7 patients did not present any alterations in parotid and submandibular glands. Limitations- Although ultrasonography exam is adequate to investigate the presence and status of major salivary glands, other important glands such as sublingual and minor mucous salivary glands are not well-visualized using this technique. Conclusions- Aplasia or hypoplasia of the major salivary gland is strongly related to ectodermal dysplasia and we suggest routine evaluation of the major salivary gland using ultrasound to monitor and manage the possible impact of xerostomia in oral health.
Costa, Thiago Roncini Gomes da. "A família IRM de moléculas de adesão celular durante a histólise da glândula salivar larval de Drosophila melanogaster." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/17/17136/tde-26042018-162637/.
Full textABSTRACT: The complex Irre Recognition Module (IRM) is an important subgroup of transmembrane proteins of the immunoglobulin superfamily that play a role in cell adhesion and participates in several processes of development of Drosophila melanogaster, such as: axonal targeting, fusion of myoblasts, spacing of sensory organs , autophagy of the salivary glands, elimination of supplementary cells and specification of cellular target in the pupal retina. The IRM family is composed of four well-characterized members: roughest (rst; irregular-chiasmC); kin-of-irre (kirre); hibris (hbs) and sticks-and-stones (sns). A striking feature of this group is the occurrence of functional redundancy during the process of fusion of myoblasts in the embryonic somatic musculature and in the phase of cell sorting of the interomatid cells in the final step of standardization of the pupal retina of Drosophila. In this context, it can be concluded that it can not provide an absence or decrease the mRNA expression levels of maintaining the wild tissue phenotype. In larval salivary glands of Drosophila, a widely used model for the study of programmed cell death (MCP), rstD mutants, a semidominant rst allele, showed persistence phenotype of this tissue after 12 hours for its observed elimination in wild animals. During the development of Drosophila melanogaster tissues, we evaluated a correlation between the 4 members of this group during the autophagy process of the salivary gland. To that end, transcription levels of complex genes were analyzed by RT-qPCR after patient formation until the end of the histolysis process. We have seen a transcriptional decrease in the first unpronounced postdoc peak of ecdysone leading to histolysis of the gland in wild animals, and altered levels of mRNAs of the complex members in rstD mutant animals. However, a first overexpression was not enough to generate persistent glands. In addition, we describe a spatial location, by immunohistochemistry, of two members of the complex, emphasizing a first and second hand colocalization, unlike the sns and hbs members.
Francesquini, Fernanda de Camargo. "Avaliação da especificidade do efeito da saliva do flebotomíneo vetor sobre a infectividade da espécie de Leishmania: infecção experimental de Leishmania (L.) amazonensis e Leishmania (V.) braziliensis com a saliva de Lutzomya flaviscutellata e Lutzomyia (Psychodopygus) complexus em camundongo BALB/c." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/5/5160/tde-20052014-104624/.
Full textDuring the natural transmission of leishmaniasis, the infected female phlebotomine regurgitates promastigotes into the host\'s skin together with the saliva. It has been reported that components of vector saliva contain immunomodulatory properties that facilitate the establishment of infection in the host, however the most studies employed salivary gland lysate (SGL) of laboratory colonized vectors. Thus, the main objective of this study was to evaluate the specificity of SGL of the phlebotomines Lutzomyia flaviscutellata and Lutzomyia (Psychodopygus) complexus caught in the field in the infectivity of L. (L.) amazonensis and L. (V.) braziliensis. BALB/c mice were inoculated in the hind footpad with promastigotes of L. (L.) amazonensis and L. (V.) braziliensis in the absence or presence of L. flaviscutelata, and L. (P.) complexus SGL. The evolution of the lesion size was evaluated weekly and biopsies from the site of infection were collected for histopathological analysis and determination of parasite load in the 4th and 8th week post infection (PI), and the draining lymph node to characterize subsets of T cells by flow cytometry. The draining lymph nodes cells were also cultured with specific antigen to determine the cytokines (IL-10, IL-12 and IL-4) in the supernatant. L. (L.) amazonensis and L. (V.) braziliensis infection was not exacerbated in the groups co-inoculated with the SGL of both species in the control group, only inoculated with the parasite. The lesion size and parasite burden of the control group was higher or equal to the groups with saliva. In L. (L.) amazonensis infection there was a decrease of CD4+ cells and an increase in the population of CD8+ cells while in L. (V.) braziliensis infection there was a maintenance of CD4+ cells and an increase in the population of CD8+ cells in all groups compared with the health group. The production of IL-10 and IL-12, did not differ between groups, as well as the production of IL-4 in the group infected by L. (V.) braziliensis. However, in the groups infected only with L. (L.) amazonensis in the presence of L. flaviscutellata saliva, it was observed a higher production of IL-4 in relation with the health group. As a whole, the results show that the saliva of L. flaviscutellata and L. (P.) complexus, in the natural vector/parasite binomium or not, did not favor the establishment of the infection caused by L. (L.) amazonensis and L. (V.) braziliensis in BALB/c mice
Leirião-Riva, Fernanda Pereira. "Anatomia dos tecidos moles e glândulas salivares do sistema estomatognático de cães e gatos: enfoque anátomo-cirúrgico." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-21062006-183449/.
Full textConcerned about the knowledge of the stomatognatic system as of fundamental importance to the practitioner and surgeons to reach a precise diagnosis and institute the appropriate treatment, the present study was proposed, consisting in the dissection of the head of four canine and four feline cadavers. The objective was the description and doccumentation of the muscular structures and tegument, detailing the origin and insertion, inervation and function of each structure of the soft tissue as well as the complete description of the salivary glands for a clinical-surgical application in dentistry.
Reuterving, Carl-Olof. "Salivary glands and oral lesions in diabetes mellitus : an experimental and clinical study with special reference to the influence of metabolic control and duration of the disease." Doctoral thesis, Umeå universitet, Medicin, 1987. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-100544.
Full textS. 1-48: sammanfattning, s. 49-90: 5 uppsatser
digitalisering@umu
Cavenaghi, Fabiano Misael. "Efeitos de inibidor de protease sobre os epitélios de revestimento e glandular do rato." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/58/58137/tde-19032010-110726/.
Full textHAART had a dramatic impact on the HIV infection, however, protease inhibitor exhibit significant drug-drug interactions, and side effects. There are only few data on effects of protease inhibitors on oral tissues. We propose to observe experimental effects of ritonavir on oral epithelial tissues, covering and glandular. Wistar rats received Ritonavir twice a week for 4-8 weeks. Controls received no treatment. At the time for sacrifice, plasma were collected for evaluation of triglycerides, total cholesterol, HDL, LDL and VLDL. Were also collected skin, tongue, palate and glandular tissues Lipoproteins were evaluated and histological examination of skin, mucosal epithelium on tongue, palate and salivar submandibular glandula were made under light microscope. Morphometric methods (cariometry and stereology) were used. Data were statistically analysed by Kruskal Wallis test for multiple samples, since our data were considered not-normal. P[U] 0.05 were considered statistically significant. Our results show that protease inhibitor may be associated with small alterations in epithelial tissues, significant mostly when on longer times using the medication. The complete significance of this data has to be better understood, and other studies has to be done to define these points.
Galeano, Zioneth Judith Garcia. "Mudanças morfológicas e glandulares associadas ao polietismo etário em Polybia paulista (Hymenoptera: Vespidae)." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/59/59131/tde-23042010-163030/.
Full textThe division of labor is a central theme in the study of social insects. In bees and wasps this ability is regulated for age castes of the workers. In Apis mellifera, several authors have shown selective activation of the glands for job in colony, and reduced body weight for foraging. In social wasps age polyethism among workers is a relatively unexplored subject. We tried to identify and to describe in Polybia paulista if the bodily and glandular changes are related to the age of the wasps or to the use of the structures in the social function. Workers of different ages at which was restricted social behavior since its emergence and workers of different ages with full social development were analyzed. When Polybia paulista was in captivity, increased body mass, decreased the mass of mesosoma and decreased the salivary and mandibular glands secretion in relation to the age advancement of the wasps. While, allowing normal social development, there was an increase in body mass, the mass of the mesosoma and the salivary and mandibular glands secretion in relation to age. Apparently, social interaction activates the formation and transition of age castes in Polybia paulista. In these wasps the bodily and glandular changes observed were related to age. Probably social interaction and the use of structures within the nest are influencing the direction of such changes. As consequence, factors that influence the demography of the colony, as the development stage or the welfare of the colony, may be decisive in the age castes regulation. These results contrast with the model proposed for the Apis mellifera age polyethism and with observations of glandular activity in the social wasp Polistes versicolor.
Florezi, Giovanna Piacenza. "Xerostomia na doença do enxerto contra o hospedeiro: análise das alterações glandulares e dos níveis salivares das citocinas envolvidas nas respostas Imunológicas Th17." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/23/23139/tde-27112017-131058/.
Full textThe graft versus host disease (GVHD) is one of the biggest causes of mortality and morbidity after hematopoietic stem cells transplantation. The pathophysiology in the chronical manifestation of the disease (cGVHD), is not entirely elucidated, however the involvement of the immunological system, by means of the innate and adaptive responses are depicted in the literature concerning the disease development. The cGVHD affects multiple organs, including the salivary glands, leading to xerostomia. These alterations are under reported and mostly unknown. Therefore, this study investigated if the symptom of xerostomia in cGVHD is triggered by functional e morphological changes in minor salivary glands. To answer this inquiry specimens of biopsied labial salivary glands from patients of cGVHD and xerostomia were analyzed qualitatively and through morphometry. Specimens of biopsied salivary glands from patients with Sjögren\'s Syndrome (SS) (which is a classic model of xerostomia) and from patients with oral lichen planus (OLP) (whose clinical oral manifestations resemble the cGVHD lesions) were used as controls. Also, the cytokines related to the immunological response Th17 (important immune pathway in cGVHD pathophysiology) in the saliva of 21 cGVHD patients, 27 of SS, 10 patients of OLP and 165 healthy individuals were analyzed using the multiplex assay. The major morphological findings revealed on the salivary glands of cGVHD patients were the extensive fibrosis, periductal fibrosis, ductal and acinar atrophy. Congestion and hyaline thrombi formation were the most important vascular changes shown among these specimens. A diffuse interstitial inflammatory infiltrate was observed, with varied intensity. The SS salivary glands, however, portrayed a focal inflammatory infiltrate, with moderate to severe intensity around the excretory ducts. These ducts exhibited atrophy, ectasia, periductal fibrosis and oncocytic metaplasia. The main vascular change presented in these patients was the manifestation of vasculitis. The salivary glands from the OLP patients showed a lesser amount of alterations. The multiplex assay revealed a higher concentration of the cytokines IL-17A, IL-4, IL-17F and IL-10 in the cGVHD samples, when compared to the other groups. These cytokines are involved within the promotion of fibrosis, which endorsed the association of these secretions with the salivary glands sclerotic mechanisms. The secretion of CD40L was higher in cGVHD samples; this membrane protein is capable of amplifying the immunological response in graft rejection, besides the capacity to regulate apoptosis and activate the endothelium in thrombi formation. The cytokines IL-31, IL-23 and IL-22, also presented a higher concentration in cGHVD patients\' saliva, these secretions are actively involved in the mechanisms of lichenoid lesions in OLP, corroborating the perceived morphological changes. The comparative analysis of the morphological and salivary changes in cGVHD confirmed the correlation of Th17 immunological response within the minor salivary glands injuries and consequent xerostomia in these patients.
Rietdorf, Katja. "Wirkungen biogener Amine auf die Erregungs-Sekretions-Kopplung in der Speicheldrüse von Periplaneta americana (L.)." Phd thesis, Universität Potsdam, 2003. http://opus.kobv.de/ubp/volltexte/2005/91/.
Full textThe aim of this PhD-work was to investigate major mechanisms of excitation-secretion coupling in the salivary gland of the cockroach Periplaneta americana (L.). This salivary gland is innervated by dopaminergic and serotonergic fibres (Baumann et al., 2002). The two transmitters stimulate different processes in the gland: Dopamine (DA) stimulates the p-cells of the acini and the salivary duct cells, whereas 5-HT (serotonin) activates the p- and the c-cells of the acini, but not the salivary duct cells. Final saliva is completely protein-free after dopamine stimulation. It contains proteins, which are secreted by the c-cells of the acini, after a 5-HT-stimulation (Just & Walz, 1996). In the first part of my work I measured the electrolytic composition of the final saliva by capillary electrophoretic analysis and measured the rates of fluid secretion, in order to answer the following questions: 1.) Which transporters affect the production of primary saliva and its modification? 2.) What is the function of the transport-active salivary duct cells for the modification of the primary saliva? Electrolytic composition of the DA- and 5-HT-stimulated final saliva is not significantly different from each other, and is hypoosmotic to the Ringer used. Salivary duct cells are stimulated by DA and modify the primary saliva by a netto ion-reabsorption. My experiments also show that the duct cells, which are unstimulated during a 5-HT-stimulation of the gland, modify the primary saliva. In the next series of experiments I investigated the effects of ouabain, an inhibitor of the Na+-K+-ATPase, and bumetanide, an inhibitor of the NKCC on the rates of fluid secretion and the electrolytic composition of the final saliva. I found, that the activity of the Na+-K+-ATPase is important for the modification of DA-stimulated primary saliva during its flow through the stimulated duct system. In contrast, it is not important for modification of the 5-HT-stimulated primary saliva. Inhibition of the Na+-K+-ATPase does not affect rates of DA-stimulated fluid secretion, but it increases the rates of 5-HT-stimulated fluid secretion. Activity of the NKCC is important for both secretory processes: the ion and the fluid secretion. Inhibition of the NKCC results in a significant drop in the rates of fluid secretion after DA- and 5-HT-stimulation, as well as a drop in electrolytic concentrations in the saliva. In the second part of my work, I tried to measure changes in the intracellular ionic concentrations (Ca2+, Na+, and K+) within the acinar cells during a DA- or 5-HT-stimulation. The experiments should be performed by ratiometric imaging. Measurements with the Ca2+-sensitive dye Fura-2 did not show any global increase in the intracellular Ca2+-concentration in the p-cells of the acini. Problems concerning a bad loading of the cells, a strong autofluorescence which changed during the time course of the stimulation, as well as changes in the cell volume were the reason, that no measurements using Na+- or K+-sensitive dyes were performed. In the third part of my work I investigated the intracellular signalling pathways, which activate protein secretion after 5-HT-stimulation of the gland. A modified Bradford Assay was used for measuring the protein content in the final saliva. In a dose-response curve I showed that rates of protein secretion are dependent on the 5-HT-concentrations used to stimulate the glands. In another set of experiments I increased the intracellular concentrations of Ca2+, cAMP and / or cGMP, and measured the protein content in the final saliva. An increase in the intracellular Ca2+-concentration activates only a low rate of protein secretion. After an increase in the intracellular cAMP-concentration a much higher rate of protein secretion can be activated, which is not significantly different from the 5-HT stimulated rate of protein secretion. The cAMP-stimulated protein secretion can be further increased by a simultaneous rise in the intracellular Ca2+-concentration. In contrast, cGMP does not activate protein secretion. Therefore I propose the expression of an adenylyl cyclase activating 5-HT-receptor in the basolateral membrane of the protein secreting c-cells.
Lamers, Marcelo Lazzaron. "Concentração elevada de glicose e interação célula-matriz extracelular: efeitos sobre a homeostase de glândulas salivares, adesão e migração celular." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-11112008-131435/.
Full textIn this study we evaluated the effects of DM on two different systems: the rat parotid gland and in vitro cultured cells. Extracellular matrix composition and the migratory behavior of cells exposed to a high glucose concentration (HG) were evaluated, respectively. In the parotid, DM led to an increase in collagens III, IV and V, laminin and fibronectin, through a TGFb2-dependent mechanism. In cultured cells, HG impaired cell polarization, reduced migration velocity and directionality, reduced the persistence and stability of protrusive cellular processes, as well as adhesion maturation. These effects were related to Rac1 GTPase activation, dependent on the oxidative stress promoted by HG. This study suggests, for the first time, that: 1) salivary hypofunction in DM might involve the thickening of capillary and parenchyma basal lamina, through mechanisms already described in other target organs for diabetic complications and 2) that glucose directly impairs cell migration, and this effect may contribute to the chronic wound healing observed in diabetic patients.
Ramos, Anderson Daniel. "Imunomodulação da encefalomielite autoimune experimental pelo extrato da glândula salivar de Aedes aegypti." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-12122014-124408/.
Full textThe saliva of hematophagous insects has molecules that can modulate the host immune system. Based on the literature about activities found in Aedes aegypti saliva, we investigate if SGE of this species could modulate EAE. We have immunized C57BL/6 mice with MOG35-55, and carried out a treatment with SGE. The treatment with SGE reduced the incidence of disease and caused a delay onset of clinical signs making them softer. We have observed that modulation occured in the induction phase of immune response, not in effector phase. In fact, SGE can suppress the disease by four ways: 1) decreasing the expression of MHCII, CD80 and CD86 in dendritic cells and decreasing the production of cytokines responsible for Th1/Th17 response induction; 2) inducing cells producing IL-10 in vivo; 3) inducing apopotosis in naive T lymphocytes; 4) inducing cells Th2 producing IL-4 e IL-5. We came to the conclusion that SGE can act in supressing symptoms during the course of EAE and inhibiting the beggining of autoimmune response.
Souza, Gabriely Köerich. "Morfologia de ovos, glândulas salivares e sistemas digestivo e reprodutor de Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)." Universidade Federal de Viçosa, 2012. http://locus.ufv.br/handle/123456789/3961.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
The bronze bug Thaumastocoris peregrinus Carpintero & Dellapé (Hemiptera: Thaumastocoridae) is a pest of Corymbia and Eucalyptus species and there are no effective strategies for its control in commercial plantations. Biological data of insects of the family Thaumastocoridae are scarce, mainly with species of the subfamily Xylastodorinae. Morphological studies of eggs and digestive and reproductive systems can provide important information to control T. peregrinus. This study described the morphology of eggs, salivary glands, alimentary canal and reproductive systems of males and females T. peregrinus. Thirty females and fifteen males of T. peregrinus with different ages were obtained from a mass rearing facility in a room with temperature at 25 ± 2ºC, 70 ± 10% RH and 12 h photophase and prepared for analysis in a light microscopy. Eggs of T. peregrinus were obtained from the mass rearing facility, coated with gold and analyzed in a scanning electron microscope. Thaumastocoris peregrinus has a pair of bilobed principal salivary glands located between the segments of the meso- and metathorax. The wall of the lobes is formed by a single layer of cubic cells with some of them binucleate. Each gland has a main duct formed by a single layer of flattened cells. The alimentary canal of T. peregrinus has a short faringe followed by a long and thin esophagus that ends in a simple proventriculus and a short estomodeal valve. The midgut of T. peregrinus comprises an anterior dilated region without gastric cecae, a long and narrow middle region and a short and dilated posterior one. The hindgut is composed by a transition region with the midgut where the insertion of two pairs of Malpighian tubules occurs, a short ileum and a rectum with a folded epithelial wall, thick cuticle and flattened cells. The male reproductive system of T. peregrinus is formed by a pair of testa with three large and globular follicles isolated by a peritoneal sheath and two pairs of tubular mesadenian accessory glands well developed. The female reproductive system of T. peregrinus is formed by a pair of ovaries with two meroistic telotrophic ovarioles each. The ovarioles open into two long lateral oviducts that join into a short common oviduct. The common oviduct ends in a large bursa copulatrix with many folds and both are lined by a thick cuticle. Eggs with embryos at the stages of anatrepsis, katatrepsis and also after the dorsal closure were observed in the reproductive tract of T. Peregrinus females. Eggs of T. peregrinus are oval-shaped and flattened dorso-ventrally with a strong depression in the center and black colored. The chorion of the egg is divided into exochorion, endochorion, air layer and an inner chorionic layer. The outer surface of the exochorion lacks perforations or pores and showed shallow and elongated depressions that were more circular in the posterior region of the egg. The opercular opening is located laterally in the eggs of T. peregrinus with the neck region showing around 27 circular and concave projections that appear to be aeromicropyles, reduced chorionic rim and a narrow sealing bar internally. The operculum is elliptical and flattened and has the same texture as the rest of the corium, except for the smooth texture of the outer opercular region. Thaumastocoris peregrinus has digestive and reproductive systems similar to other species of Thaumastocoridae, but the external characteristics of the eggs of this species differ from others of the same family. Studies of a larger number of species can contribute to the knowledge and importance of the structures evaluated for identifications at the level of subfamily, genus or species and to understand the phylogenetic relationships of Thaumastocoridae.
O percevejo bronzeado Thaumastocoris peregrinus Carpintero & Dellapé (Hemiptera: Thaumastocoridae) é praga de espécies de Corymbia e Eucalyptus e não existem estratégias eficientes para seu controle em plantios comerciais. Dados biológicos de insetos da família Thaumastocoridae são escassos, sendo, principalmente,com espécies da subfamília Xylastodorinae. Estudos morfológicos dos ovos e sistemas digestivo e reprodutor podem fornecer informações para o controle de T. peregrinus. Este estudo descreveu a morfologia dos ovos, das glândulas salivares, do canal alimentar e dos sistemas reprodutores de machos e fêmeas de T. peregrinus. Trinta fêmeas e quinze machos de T. peregrinus com diferentes idades foram obtidos de criação massal em sala climatizada à temperatura de 25 ± 2oC, 70 ± 10% de UR e fotofase de 12 horas e preaparados para análise em microscópio de luz. Ovos de T. peregrinus foram obtidos da criação massal, metalizados com ouro e analisados em microscópio eletrônico de varredura. Thaumastocoris peregrinus possui um par de glândulas salivares principais bilobadas entre os segmentos do meso e metatórax. As paredes dos lóbulos são formadas por uma camada simples de células cúbicas sendo algumas binucleadas. Cada glândula possui um ducto principal formado por uma camada simples de células achatadas. O canal alimentar de T. peregrinus é formado por uma faringe curta seguida por um esôfago longo e fino terminando em um proventrículo simples e uma válvula estomodeal curta. O intestino médio de T. peregrinus é composto por uma região anterior dilatada e sem cecos gástricos, uma região mediana longa e estreita e outra posterior curta e dilatada. O intestino posterior é composto por uma região de transição com o intestino médio onde ocorre a inserção de dois pares de túbulos de Malpighi, um curto íleo e um reto com parede epitelial com grande quantidade de dobras, cutícula espessa e células achatadas. O sistema reprodutor masculino de T. peregrinus é formado por um par de testículos com três folículos grandes e globulares isolados por uma bainha peritoneal e dois pares de glândulas acessórias mesadênias tubulares e bem desenvolvidas. O sistema reprodutor feminino de T. peregrinus é formado por um par de ovários com dois ovaríolos cada do tipo meroístico telotrófico. Os ovaríolos abrem-se em dois ovidutos laterais longos que se unem em um oviduto comum curto. O oviduto comum termina em uma bursa copulatrix ampla e com diversas dobras e ambos são revestidos por uma cutícula espessa. Ovos com embriões nos estágios de anatrepsis, catatrepsis e após o fechamento dorsal foram observados no trato reprodutivo de fêmeas de T. peregrinus. A forma dos ovos de T. peregrinus é oval e achatada dorso-ventralmente com forte depressão no centro e coloração preta. O cório do ovo é dividido em exocório, endocório, camada de ar e camada coriônica interna. A superfície externa do exocório apresentou depressões pouco profundas e alongadas ou com formato mais circular na região posterior do ovo sem perfurações ou poros. A abertura opercular é localizada lateralmente no ovo de T. peregrinus com a região do colo apresentando em torno de 27 projeções circulares côncavas que parecem ser aeromicrópilas, borda corial reduzida e, internamente, uma faixa de vedação estreita. O opérculo é elíptico e achatado e apresenta textura semelhante ao restante do cório, exceto pela textura lisa da região mais externa. Thaumastocoris peregrinus possui sistemas digestivos e reprodutor semelhantes a outras espécies de Thaumastocoridae, mas as características externas dos ovos diferiram das demas espécies dessa família. Estudos de um maior número de espécies poderão contribuir para o conhecimento e importância das estruturas avaliadas para identificações em nível de subfamília, gênero ou espécie e se compreender as relações filogenéticas de Thaumastocoridae.
Oliveira, Elaine Cyreno. "Adesão e atividade de protease são reguladas pelo peptídeo derivado da laminina AG73, sindecan-1 e integrina 1 em linhagem celular derivada de carcinoma adenóide cístico." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-09022010-105201/.
Full textWe studied induction of MMP activity by b1-laminin peptide AG73 in adenoid cystic carcinoma cell line (CAC2). Cells grown inside AG73-enriched laminin-111 exhibited pseudocystics spaces. MMP inhibitor decreased those spaces, suggesting MMPs action. Cells grown on AG73 showed a dose-dependent increase of MMP9 secretion. MMP9 siRNAi decreased remodeling in 3D culture. We searched for AG73 receptors regulating MMP9 activity. CAC2 grown on AG73 exhibited colocalization of syndecan-1 and b1 integrin. Syndecan-1 siRNA or siRNA b1 integrin showed reduction in adhesion to AG73 and in remodeling and protease activities. Double-knockdown explored syndecan-1 and 1 integrin cooperation and showed decrease in adhesion to AG73 and in MMP activity. Receptors characterization was made by affinity chromatography followed by mass spectrometry through AG73-affinity columns and showed putative receptors, like b1 and aV integrins. We suggest that AG73 peptide regulates adhesion and MMP secretion in CAC2 cells through syndecan-1 and b1 integrin.
Miguel, M?rcia Cristina da Costa. "Express?o imuno-histoqu?mica das integrinas a2?1, a3?1 e a5?1 em adenoma pleom?rfico de gl?ndula salivar menor e maior e carcinoma aden?ide c?stico." Universidade Federal do Rio Grande do Norte, 2005. http://repositorio.ufrn.br:8080/jspui/handle/123456789/17167.
Full textConselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
Pleomorphic adenoma and adenoid cystic carcinoma (ACC) consist benign and malignant neoplasm from salivary gland, respectively. These neoplasms share some characteristics, such as cellular origin and considerable production of extracellular matrix, however, with distinct biological behavior. The aim of the present study was to compare the expression of D2E1, D3E1 e D5E1 integrins in pleomorphic adenoma from minor and major salivary glands and ACCs. Furthermore, it was investigated possible differences in the expression of these integrins according to histological subtypes of ACC. Fourteen cases of pleomorphic adenoma from major salivary gland, fourteen cases from minor salivary gland and ten cases of ACC were selected. It was taken into consideration the presence or absence, localization and intensity of integrin immunoexpression. The cases of pleomorphic adenoma were grouped in order to compare the expression between the distinct neoplasms. It was observed a highly significant difference (p<0,0001) in relation to D2E1 integrin between the neoplasms since pleomorphic adenoma showed a pronounced immunostaining. It was not possible to perform statistical tests considering the D2E1 integrin expression; nevertheless, it could be observed a tendency of higher staining in pleomorphic adenoma. For comparative reasons the cases ACCs were divided in two groups: solid and tubular/cribriform. It was not detected significant differences in regard to D2E1 integrin; and statistical analysis could not be realized in relation to D3E1 and D5E integrin expression. However, it was also verified a tendency of absence or reduced expression in the solid subtype. It can be concluded that the reduced D2E1 integrin expression observed in CACs may be related to a lesser degree of cell differentiation in this neoplasm and the reduced D5E1 integrin expression can be associated with aggressive biological behavior. Moreover, the absence and/or reduced expression of the studied integrins in solid ACC suggests a role in pathogenesis and more aggressive biological behavior of this histological subtype
O adenoma pleom?rfico e o carcinoma aden?ide c?stico (CAC) representam neoplasias de gl?ndula salivar benigna e maligna, respectivamente, as quais compartilham algumas caracter?sticas como a mesma origem celular e uma marcante presen?a de matriz extracelular, apresentando, por?m, comportamentos biol?gicos distintos. O prop?sito desta pesquisa consistiu em comparar a express?o das integrinas D2E1, D3E1 e D5E1 em adenomas pleom?rficos de gl?ndula salivar menor e maior e CACs. Al?m disso, procurou investigar se havia diferen?as na express?o destas integrinas entre os subtipos histopatol?gicos do CAC. Foram selecionados 14 casos de adenoma pleom?rfico de gl?ndula salivar maior, 14 casos de gl?ndula salivar menor e 10 casos de CACs. Analisou-se a presen?a ou aus?ncia, localiza??o e intensidade de marca??o das integrinas. Os dois grupos de adenomas pleom?rficos foram reunidos em um s? para fazer a compara??o entre os dois tumores. Verificou-se que houve diferen?a estat?stica altamente significativa (p<0,0001) para a integrina D2E1 entre os dois tumores, apresentando o adenoma pleom?rfico, uma marca??o mais intensa para esta integrina. Em rela??o ? integrina D5E1 n?o foi poss?vel a realiza??o de testes estat?sticos, ficando patente, por?m, que houve uma tend?ncia da referida integrina ser mais intensamente expressa no adenoma pleom?rfico. Para an?lise comparativa, os CACs foram subdivididos em 2 grupos: s?lido e tubular/cribriforme. Para a integrina D2E1 observou-se que n?o houve diferen?a estatisticamente significativa e em rela??o ? D3E1 e D5E1 n?o foi poss?vel a realiza??o do teste estat?stico; no entanto, tamb?m foi verificada uma clara tend?ncia para os casos do subtipo s?lido apresentarem express?o ausente ou reduzida das integrinas avaliadas. Concluiu-se que a reduzida express?o da integrina D2E1 observada nos CACs, pode estar relacionada com a menor diferencia??o das c?lulas deste tumor e ? poss?vel que a reduzida express?o da D5E1, possa estar implicada em seu comportamento mais agressivo. Al?m disso, sugere-se que a aus?ncia e/ou redu??o da express?o das integrinas pesquisadas nos casos do subtipo s?lido, pode desempenhar algum papel na patog?nese e no comportamento biol?gico mais agressivo deste subtipo tumoral
Fernandes, K?tia Roberta. "Biologia, Diagn?stico morfol?gico e molecular da infec??o experimental e natural de Babesia equi (Laveran, 1901) em Boophilus microplus (Canestrini, 1887)." Universidade Federal Rural do Rio de Janeiro, 2007. https://tede.ufrrj.br/jspui/handle/tede/819.
Full textCoordena??o de Aperfei?oamento de Pessoal de N?vel Superior
The aim of the present study was to evaluate the natural and experimental infection of B. equi in nymphs and adult Boophilus microplus using optical microscopy and molecular analysis. The experimental infection was observed in nymphs, males and females of B. microplus fed on equines chronically infected with B. equi and in non fed larvae and eggs. For the experiments two equines, of not defined breed, proven to be infected with B. equi were used. The animals were infested with B. microplus larvae of generation F4 obtained from a laboratory maintained population, known to be free of Babesia spp. infection. Daily collections of ticks were realized using as starting point the ecdisys to nymph state. After the collection the hemolymph was examined and the intestine and salivary glands were dissected, smashed on glass slides for microscopy, methanol treated and stained with Giemsa. There were dissected 860 specimens (432 nymphs, 280 females and 148 males). The dissected salivary glands were divided into two parts. The first one was smashed on microscopy glass slides, stained and examined by optical microscopy for morphologic analyses. From the second part was realized DNA extraction and PCR with specific primers for the 18S rRNA. On optical microscopy of nymph hemolymph was possible to be observed the presence of kinetes with claviform aspect characteristic for the genus Babesia. In the salivary glands of B. microplus nymphs the morphology and the sequence of developmental stages of B. equi were observed initiating on day 4 after ecdysis, being possible to see in acinary cells the formation of sporoblasts and ovoid sporozoites measuring 1.5 μm of diameter and elongated sporozoites with 3.8 to 5.2μm of length and 0.8 to 1.5 μm of width. The PCR confirmed the presence of B. equi in DNA samples extracted from salivary glands of nymphs, male and female ticks as well as in larvae and eggs. To evaluate the natural infection were collected nymphs and adult B. microplus from two equines naturally infested by these ticks and naturally infected with B. equi. These horses were originated from Seropedica city in the state of Rio de Janeiro. There were dissected 324 specimens (145 nymphs, 138 females and 41 males). The proceedings with the salivary glands were identical to the previously described for the experimental infection. Of the salivary glands submitted to PCR, 70% showed to be infected with B. equi. Microscopical analysis of the salivary glands revealed the presence of sporoblast stages and the development of elongated sporozoites in acinary glands. The morphologic, morphometric and molecular analysis confirmed the experimental and natural infection of the salivary glands of nymphs and adult B. microplus with B. equi. The results of the present study show the ability of B. equi to develop in this tick species. The detection of B. equi DNA in eggs and larvae also suggests the possibility of transovarian transmission in B. microplus. The results allow to consider the tick B. microplus as a potential biologic vector of B. equi in horses from the studied region.
Este trabalho teve como objetivo avaliar a infec??o natural e experimental de Babesia equi em ninfas e adultos de Boophilus microplus por meio de microscopia ?ptica e an?lise molecular. A infec??o experimental foi observada a partir de ninfas, machos, f?meas, ovos e larvas n?o alimentadas de B. microplus alimentados em equinos com infec??o cr?nica por B. equi. Para a realiza??o do experimento foram utilizados dois eq?inos, mesti?os, com infec??o por B. equi. Os animais foram infestados com larvas de B. microplus de gera??o F4 obtidas de col?nia mantida em laborat?rio e livres de infec??o por Babesia spp. A partir da ecdise para ninfa foram realizadas coletas di?rias. Ap?s a coleta foram realizados os exames de hemolinfa e extra??o do intestino e das gl?ndulas salivares os quais foram macerados em l?minas de vidro para microscopia, fixados em metanol e corados com Giemsa. As gl?ndulas salivares dissecadas foram divididas em duas partes. A primeira parte foi macerada em l?minas de vidro para microscopia, corada com corante Giemsa e observada em microsc?pio ?tico para an?lise morfol?gica. A segunda parte foi realizada a extra??o de DNA, sendo submetida a PCR com primers especif?cos para o gene 18S rRNA para B. equi. Foram dissecados 860 esp?cimes (ninfas= 432; f?meas= 280 e machos= 148). Na microscopia ?ptica foi poss?vel observar nas hemolinfas das ninfas a presen?a de cinetos com aspecto claviforme t?picos do g?nero Babesia. Nas gl?ndulas salivares, a morfologia e a seq??ncia dos est?gios de desenvolvimento de B. equi das ninfa s de B. microplus, foram observadas a partir do 4? dia ap?s ecdise, sendo poss?vel observar nos ?cinos celulares a forma??o de esporoblastos e de esporozo?tas ov?ides medindo 1,5 μm de di?metro e alongados medindo 3,8 a 5,2μm de comprimento e 0,8 a 1,5μm de largura. A rea??o em cadeia de polimerase (PCR) confirmou a presen?a de B. equi em DNA de gl?ndulas salivares extra?das de ninfas, machos, f?meas, assim como dos ovos e larva. A infec??o natural foi observada a partir de ninfas e adultos de B. microplus coletados de dois eq?inos naturalmente infestados e comprovadamente infectados por B. equi, procedentes do munic?pio de Serop?dica, Rio de Janeiro. Foram dissecados 324 esp?cimes (ninfas= 145, f?meas= 138 e machos= 41). O processamento das gl?ndulas salivares dissecadas foi semelhante ao descrito para infec??o experimental. Das gl?ndulas salivares submetidas a PCR, 70% apresentaram resultados positivos para B. equi. As an?lises por microscopia ?ptica das gl?ndulas salivares das ninfas e dos adultos revelaram a presen?a nos ?cinos celulares os est?gios de esporoblastos e o desenvolvimento de esporozo?tas alongados. As an?lises morfol?gicas, morfom?tricas e moleculares confirmaram a infec??o experimental e natural das gl?ndulas salivares de ninfas e adultos de B. microplus por B. equi. Os resultados deste estudo demonstram a capacidade de multiplica??o de B. equi em gl?ndulas salivares de ninfas e adultos de B. microplus. A detec??o de DNA de B. equi em ovos e larvas de B. microplus tamb?m sugere a possibilidade da transmiss?o transovariana nesta esp?cie de carrapato. Estes resultados sugerem que o carrapato B. microplus ? vetor biol?gico de B. equi na regi?o estudada.
Lascane, Nelise Alexandre da Silva. "Expressão imuno-histoquímica das proteínas Jab1, p27, c-jun e c-fos no adenoma pleomórfico, adenocarcinoma polimorfo de baixo grau e carcinoma adenoide cístico das glândulas salivares." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/23/23141/tde-18032015-173058/.
Full textSalivary gland tumors comprise about 2 to 6.5% of the head and neck tumors. Among the salivary gland tumors, pleomorphic adenoma is the most common and benign tumor. Adenoid cystic carcinoma and polymorphous low-grade adenocarcinoma are the most frequent malignant tumors. Jab1 is one of many proteins which affects many stages of the tumorigenesis and regulates positively and negatively several pathways and/or proteins such as p27 and AP-1, the latter composed by c-jun and c-fos, which are mostly related to cell cycle and cell proliferation. The aim of this study was to evaluate the immunoexpression of the proteins Jab1, p27, c-jun and c-fos in pleomorphic adenoma, polymorphous low-grade adenocarcinoma and adenoid cystic carcinoma of the salivary glands. The semi-quantitative immunohistochemical analysis was performed in salivary gland tumors and in normal salivary gland according to the score 0 (no stained cells), 1 (> 0 <= 5% of stained cells), 2 (> 5 <= 50%) and 3 (> 50%). Nuclear immunostaining alone was considered for Jab1, c-jun and c-fos proteins and cytoplasmic and nuclear staining for p27. Results were analyzed in GraphPad Prisma 5.0 software using Kruskal-Wallis, Mann-Whitney and Chi-square tests and Spearman correlation test in which significancy level was p<0,05. Statistical analysis revealed that Jab1 expression was significant in pleomorphic adenoma and adenoid cystic carcinoma in relation to ducts and in polymorphous low-grade adenocarcinoma in relation to adenoid cystic carcinoma (p=0,0136, 0,0001 e 0,0344, respectively); the p27 nuclear expression was significant in pleomorphic adenoma and in polymorphous low-grade adenocarcinoma when compared to adenoid cystic carcinoma (p=0,0074 e 0,0004, respectively) and cytoplasmic immunostaining was significant in all groups when compared to acini; c-fos expression was significant in ducts if compared to pleomorphic adenoma, polymorphous low-grade adenocarcinoma and adenoid cystic carcinoma (p=0,0002, 0,0048 e 0,0352, respectively). Spearman correlation test to Jab1, p27, c-jun and c-fos in each lesion separately revealed significant correlation between Jab1 and p27 (r=0,371; p=0,020) and c-jun and c-fos (r=0,452; p=0,004) in pleomorphic adenoma. There was correlation between Jab1 and p27 (r=0,494; p=0,044) in polymorphous low-grade adenocarcinoma and between p27 and c-fos (r=0,513; p=0,035) in adenoid cystic carcinoma. In conclusion, tumorigenesis in pleomorphic adenoma, polymorphous low-grade adenocarcinoma and adenoid cystic carcinoma seems to be associated to expression of Jab1 and p27.
Hira, Priyesh Gunvant. "Buffering capacity of saliva, salivary flow rates and cortisol levels in patients with active caries." Thesis, 2013. http://hdl.handle.net/10539/12545.
Full textMendes, Susete Maria Oliveira. "Hipossalivação: da etiologia ao tratamento." Master's thesis, 2017. http://hdl.handle.net/10284/6114.
Full textThe dry mouth sensation can have a subjective nature, called xerostomia, or it can be associated to salivary gland hypofunction (hyposalivation) or a change in the composition of saliva. Despite their indiscriminate use, these are two different concepts, which are often underrated and subdiagnosed by the dentist. Patients with salivary gland hypofunction are subject to various complications, such as dysgeusia, pain, a burning sensation in the oral mucosa, dental caries and other infectious diseases, dysphagia and dysphonia, which are common complaints in the dental medicine appointment. So far, there hasn’t been a general consensus over the treatment options for a patient with hyposalivation, therefore further research is necessary in order to achieve a uniformization.