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Journal articles on the topic 'GLRaV'

Author: Grafiati

Published: 4 June 2021

Last updated: 2 February 2022

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1

Türkmen, Yağmur, and Filiz Ertunç. "Determination of Grapevine Leafroll Diseases Infection in Turkey." Turkish Journal of Agriculture - Food Science and Technology 7, no. 11 (2019): 1947. http://dx.doi.org/10.24925/turjaf.v7i11.1947-1953.2913.

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During the growing seasons between 2009 and 2010, totally 213 shoot and leaf samples were collected from the vineyards in Manisa, Denizli, Nevşehir, Elazığ, İzmir, Ankara, Çanakkale, Tekirdağ, Edirne and Kırklareli, which are commercially important viticulture production areas of Turkey. All grapevine samples were serologically investigated for the presence of Grapevine leafroll-associated viruses (GLRaVs) using GLRaV-1, GLRaV-2, GLRaV-3, GLRaV 4-9, GLRaV-6 and GLRaV-7 DAS-ELISA kits. In 143 of total, (67.14%) single or multiple infections were detected. GLRaV4-9, -7, -3, -1, -2 and -6 were de

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Tsai, C. W., J. Chau, L. Fernandez, D. Bosco, K. M. Daane, and R. P. P. Almeida. "Transmission of Grapevine leafroll-associated virus 3 by the Vine Mealybug (Planococcus ficus)." Phytopathology® 98, no. 10 (2008): 1093–98. http://dx.doi.org/10.1094/phyto-98-10-1093.

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Grapevine leafroll disease is caused by grapevine leafroll-associated viruses (GLRaVs). Within this virus complex, GLRaV-3 is the predominant species in the world. Several GLRaVs have been shown to be transmitted from vine to vine by mealybugs although a detailed characterization of transmission biology is lacking. The introduction of the vine mealybug (Planococcus ficus) in California and other regions of the world may result in increasing disease incidence of established GLRaVs. We studied the characteristics of GLRaV-3 transmission by the vine mealybug. Our results indicate that the vine me

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3

Štrukelj, Melita, Jaka RAZINGER, Barbara GRUBAR, et al. "Fiziološki odziv žlahtne vinske trte Vitis vinifera L. na okužbo z zvijanjem listov vinske trte povezanih virusov (GLRaV-1 in GLRaV-1 + GLRaV-3)." Acta agriculturae Slovenica 107, no. 2 (2016): 519. http://dx.doi.org/10.14720/aas.2016.107.2.22.

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Grapevine leafroll disease is one of the most severe viral diseases of grapevine caused by Grapevine leafroll-associated viruses (GLRaVs). Physiological processes were monitored on grapevines with single (GLRaV-1) and mixed (GLRaV-1 and GLRaV -3) viral infection under greenhouse conditions from June to September, in vegetation period 2014. In the mid of the season (July) negative effects of the virus infections on physiological processes were more severe in mixed than in single infection. The net-photosynthesis (Pn) of the leaves infected with GLRaV-1 and GLRaV-3 reached only a half of the Pn

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Poojari, S., J. Boulé, N. DeLury, et al. "Epidemiology and Genetic Diversity of Grapevine Leafroll-Associated Viruses in British Columbia." Plant Disease 101, no. 12 (2017): 2088–97. http://dx.doi.org/10.1094/pdis-04-17-0497-re.

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Grapevine leafroll disease (GLD) is a complex associated with one or more virus species belonging to the family Closteroviridae. The majority of viruses in this complex are vectored by one or more species of mealybugs (Pseudococcidae) and/or scale insects (Coccidae). Grape-growing regions of British Columbia (BC), including Okanagan, Similkameen, and Fraser valleys and Kamloops (BC central interior), Vancouver, and Gulf islands, were surveyed during the 2014 and 2015 growing seasons for the presence of four major grapevine leafroll-associated viruses, including Grapevine leafroll-associated vi

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5

Liu, M. H., M. J. Li, H. H. Qi, et al. "Occurrence of Grapevine Leafroll-Associated Viruses in China." Plant Disease 97, no. 10 (2013): 1339–45. http://dx.doi.org/10.1094/pdis-01-13-0048-re.

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To characterize the prevalence of viruses associated with grapevine leafroll disease in China, 249 grapevine (Vitis spp.) samples (86 popular cultivars and a rootstock) from 19 provinces and regions were collected and tested for Grapevine leafroll-associated virus 1 (GLRaV-1), GLRaV-2, GLRaV-3, GLRaV-4, and GLRaV-4 strain 5 by SYBR Green real-time reverse-transcription polymerase chain reaction (RT-PCR), and RT-PCR and sequencing. GLRaV-3 was found in 100% of the samples while GLRaV-1, GLRaV-2, and GLRaV-4 were detected in 24.9% (62/249), 15.3% (38/249), and 0.80% (2/249) of the samples, respe

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6

Kumar, Priyadarsini, and Stanley Meizel. "Identification and spatial distribution of glycine receptor subunits in human sperm." REPRODUCTION 136, no. 4 (2008): 387–90. http://dx.doi.org/10.1530/rep-08-0223.

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The human sperm surface glycine receptor (GLR) plays a role in an important fertilization event, the sperm acrosome reaction. Here, by western blot analysis, we report the presence of GLRA1, GLRA2, GLRA3, and GLRB subunits in human sperm. Immunolocalization studies showed that the GLRA1 and GLRA2 subunits are present in the equatorial region, the GLRA3 subunit in the flagellar principal piece, and the GLRB subunit in the acrosomal region of sperm. This first demonstration of isoforms of the sperm GLRA subunit and of a differential spatial distribution of the α and β subunits on the surface of

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7

Good, Xin, and Judit Monis. "Partial Genome Organization, Identification of the Coat Protein Gene, and Detection of Grapevine leafroll-associated virus-5." Phytopathology® 91, no. 3 (2001): 274–81. http://dx.doi.org/10.1094/phyto.2001.91.3.274.

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The genome of Grapevine leafroll-associated virus-5 (GLRaV-5) was cloned, and the sequence of 4766 nt was determined. Degenerate oligonucleotide primers designed from the conserved closterovirus heat shock 70 protein (HSP 70) homologue were used to obtain viral-specific sequences to anchor the cloning of the viral RNA with a genomic walking approach. The partial nucleotide (nt) sequence of GLRaV-5 showed the presence of four open reading frames (ORF A through D), potentially coding for the HSP 70 homologue (ORF A); a 51-kDa protein of unknown function with similarity to GLRaV-3 p55 (ORF B); th

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8

Jarugula, S., M. J. Soule, A. Rowhani, and R. A. Naidu. "First Report of Grapevine leafroll-associated virus-9 in Washington State Vineyards." Plant Disease 92, no. 3 (2008): 485. http://dx.doi.org/10.1094/pdis-92-3-0485c.

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Grapevine leafroll disease (GLD) has been recognized as one of the major constraints to the production of wine grapes in Washington State. At least nine distinct Grapevine leafroll-associated viruses (GLRaV-1 to -9) have been detected in grapevines showing GLD symptoms in grape-growing areas of several countries. Previous studies documented the presence of GLRaV-1, -2, and -3 in Washington State (3). We initiated a program to test grapevine cultivars with GLD symptoms for the occurrence of the other GLRaVs. Leaf samples were collected from individual grapevines of red-berried grapevine cultiva

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9

Han, J., M. A. Ellis, and F. Qu. "First Report of Grapevine leaf roll-associated virus-2 and -3 in Ohio Vineyards." Plant Disease 98, no. 2 (2014): 284. http://dx.doi.org/10.1094/pdis-03-13-0276-pdn.

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Grapevine leaf roll-associated viruses (GLRaVs) are a group of nine closely related viruses belonging to the Closteroviridae family that cause grapevine leaf roll disease in vineyards across the world (3). Within the continental United States, GLRaVs have been reported in the states of California, Michigan, Missouri, New York, Oregon, Washington, and Wisconsin, but not in Ohio (2,3). During 2012, grapevines with typical leaf roll symptoms were reported by owners of several Ohio vineyards. The symptoms included small, red leaves and downwardly rolled leaf margins, accompanied by tiny grape clus

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10

Engel, E. A., P. Escobar, C. Montt, S. Gómez-Talquenca, and P. D. T. Valenzuela. "First Report on the Occurrence of Grapevine leafroll-associated virus 7 and 9 in Chilean Grapevines." Plant Disease 92, no. 8 (2008): 1252. http://dx.doi.org/10.1094/pdis-92-8-1252c.

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Grapevine is one of the oldest horticultural crops and represents a highly valuable agricultural commodity. So far, nine distinct Grapevine leafroll-associated viruses (GLRaVs) within the Closteroviridae family have been found to be associated with grapevine leafroll disease (3). Previous studies have demonstrated a high incidence of GLRaV-1, -2, and -3 in Chile (2). To determine if other GLRaVs were present, 21 dormant cane samples were screened with a comprehensive 70-mer oligonucleotide microarray designed to simultaneously detect all grapevine viruses with total or partial genomic sequence

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11

Morales, R. Z., and J. Monis. "First Detection of Grapevine leafroll associated virus-7 in California Vineyards." Plant Disease 91, no. 4 (2007): 465. http://dx.doi.org/10.1094/pdis-91-4-0465b.

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Grapevine leafroll is one of the most important viral diseases in grapevine and occurs wherever grapevines are grown. At least nine different Grapevine leafroll associated viruses (GLRaVs) belonging to different genera within the Closteroviridae family have been reported. Typical leafroll symptoms include downward rolling and interveinal reddening or yellowing of leaves, reduced fruit size, sugar content, and yield. Our laboratory has detected the presence of GLRaV-7 in many grapevine cuttings collected in California vineyards. During the 2005-2006 fall/winter testing season, symptomatic and a

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12

Le Maguet, J., M. Beuve, E. Herrbach, and O. Lemaire. "Transmission of Six Ampeloviruses and Two Vitiviruses to Grapevine by Phenacoccus aceris." Phytopathology® 102, no. 7 (2012): 717–23. http://dx.doi.org/10.1094/phyto-10-11-0289.

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Grapevine leafroll disease is caused by grapevine leafroll-associated viruses (GLRaVs). These viruses are common in vineyards worldwide and often associated with vitiviruses that are involved in the rugose wood complex of grapevine. Ten mealybug species are known as vectors of one or several of these grapevine viruses, including the apple mealybug Phenacoccus aceris which is widespread in Holarctic regions and able to transmit Grapevine leafroll-associated virus-1 and -3 (GLRaV-1 and -3). Our aim was to characterize the transmission features of leafroll viruses by Phenacoccus aceris in order t

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13

Jones, T. J., F. Westover, and M. Nita. "First Report of Grapevine leafroll-associated virus-2 and -3 in Texas Vineyards." Plant Disease 98, no. 11 (2014): 1592. http://dx.doi.org/10.1094/pdis-06-14-0619-pdn.

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Grapevine leafroll disease (GLD), caused by the grapevine leafroll-associated viruses (GLRaVs, family Closteroviridae) is an important disease in all grapevine-growing regions of the world (2). It negatively affects vine vigor, fruit yield, and grape quality (e.g., sugar accumulation) (3). Typical disease symptoms include downward rolling of grape leaves accompanied by interveinal reddening in red-fruited varieties and interveinal chlorosis in white-fruited varieties (2). The state of Texas currently has over 275 commercial vineyards and acreage under grape production is expanding. Currently,

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14

Monis, Judit, and Richard K. Bestwick. "Serological Detection of Grapevine Associated Closteroviruses in Infected Grapevine Cultivars." Plant Disease 81, no. 7 (1997): 802–8. http://dx.doi.org/10.1094/pdis.1997.81.7.802.

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Western blot immunoassay and enzyme-linked immunosorbent assay using different monoclonal antibodies (MAb) and polyclonal antisera (PA) revealed mixed infections of serologically related and unrelated grapevine leafroll associated viruses (GLRaVs) and grapevine corky bark associated virus (GCBaV) in symptomatic grapevines. A PA designated rootstock-scion incompatibility (RSI)-24 kDa, grapevine corky bark PA, and GLRaV-2b MAb reacted to polypeptides of approximately 24 kDa isolated from grapevines exhibiting rootstock-scion incompatibility, leafroll, and corky bark disease symptoms, suggesting

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15

Pei, G. Q., Y. F. Dong, Z. P. Zhang, and X. D. Fan. "First Report of Grapevine leafroll-associated virus 4 and 5 in Grapevines in China." Plant Disease 94, no. 1 (2010): 130. http://dx.doi.org/10.1094/pdis-94-1-0130a.

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Grapevine leafroll disease (GLD) is one of the most important diseases of grapevines worldwide. Nine serologically distinct viruses in the Closteroviridae family are associated with GLD. Previous studies reported that Grapevine leafroll-associated virus (GLRaV) -1, -2, -3, and -7 were present in grapevines in China with GLRaV-1 and -3 the predominant viruses associated with GLD (1). To confirm if GLRaV-4 and -5 were also present in China, 36 dormant canes from individual vines of 29 cultivars that showed GLD leaf symptoms during the growing season were collected from the germplasm collection p

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16

Song, Yashu, Robert H. Hanner, and Baozhong Meng. "Probing into the Effects of Grapevine Leafroll-Associated Viruses on the Physiology, Fruit Quality and Gene Expression of Grapes." Viruses 13, no. 4 (2021): 593. http://dx.doi.org/10.3390/v13040593.

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Grapevine leafroll is one of the most widespread and highly destructive grapevine diseases that is responsible for great economic losses to the grape and wine industries throughout the world. Six distinct viruses have been implicated in this disease complex. They belong to three genera, all in the family Closteroviridae. For the sake of convenience, these viruses are named as grapevine leafroll-associated viruses (GLRaV-1, -2, -3, -4, -7, and -13). However, their etiological role in the disease has yet to be established. Furthermore, how infections with each GLRaV induce the characteristic dis

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17

Engel, E. A., P. F. Escobar, P. A. Rivera, and P. D. T. Valenzuela. "First Report on the Occurrence of Grapevine leafroll-associated virus 5 in Chilean Grapevines." Plant Disease 94, no. 8 (2010): 1067. http://dx.doi.org/10.1094/pdis-94-8-1067a.

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Grapevine leafroll is one of the most widespread and economically damaging viral diseases of grapevines. At least eight distinct Grapevine leafroll-associated viruses (GLRaVs), all members of the Closteroviridae family, have been associated with this disease (4). GLRaV-5 was recently reported in vineyards from Argentina (2). To determine if GLRaV-5 was present in Chilean grapevines, in addition to the previously reported GLRaV-1, -2, -3, -4, -7, and -9 (1), 45 dormant cane samples from 12 different cultivars were collected from different geographic regions of Chile and screened by reverse tran

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18

Escobar, P. F., N. Fiore, P. D. T. Valenzuela, and E. A. Engel. "First Detection of Grapevine leafroll-associated virus 4 in Chilean Grapevines." Plant Disease 92, no. 10 (2008): 1474. http://dx.doi.org/10.1094/pdis-92-10-1474c.

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Grapevine leafroll is one of the most widespread and economically relevant viral diseases of grapevines. At least nine distinct Grapevine leafroll-associated viruses (GLRaVs), all members of the Closteroviridae family, have been associated with this disease in grapevine. Grapevine leafroll-associated virus 4 (GLRaV-4), currently classified as a Closteroviridae member under the Ampelovirus genus, was initially described in California. To determine if GLRaV-4 was present in Chilean grapevines, in addition to the previously reported GLRaV-1, -2, -3, -7, and -9 (1,2), 35 dormant cane samples from

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19

Tsai, Chi-Wei, Adib Rowhani, Deborah A. Golino, Kent M. Daane, and Rodrigo P. P. Almeida. "Mealybug Transmission of Grapevine Leafroll Viruses: An Analysis of Virus–Vector Specificity." Phytopathology® 100, no. 8 (2010): 830–34. http://dx.doi.org/10.1094/phyto-100-8-0830.

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To understand ecological factors mediating the spread of insect-borne plant pathogens, vector species for these pathogens need to be identified. Grapevine leafroll disease is caused by a complex of phylogenetically related closteroviruses, some of which are transmitted by insect vectors; however, the specificities of these complex virus–vector interactions are poorly understood thus far. Through biological assays and phylogenetic analyses, we studied the role of vector-pathogen specificity in the transmission of several grapevine leafroll-associated viruses (GLRaVs) by their mealybug vectors.

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Padilla, C. V., E. Cretazzo, I. Hita, N. López, V. Padilla, and L. Velasco. "First Report of Grapevine leafroll-associated virus 5 in Spain." Plant Disease 94, no. 12 (2010): 1507. http://dx.doi.org/10.1094/pdis-07-10-0508.

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Grapevine leafroll-associated viruses (GLRaVs) cause significant reductions in yield and quality in the wine industry worldwide. At least nine different GLRaVs have been found in different regions of the world. In the process of virus indexing of candidate grapevine clones for certification, which includes grafting of scions onto rootstocks, we observed strong leafroll symptoms 1 year after grafting with one vine of cv. Estaladina in Castilla y León, Spain and one vine of cv. Tempranillo in La Rioja, Spain, collected in 2008 and 2007, respectively. Both vines tested positive by real-time rever

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Velasco, Leonardo, Josefina Bota, Rafael Montero, and Enrico Cretazzo. "Differences of Three Ampeloviruses' Multiplication in Plant May Explain Their Incidences in Vineyards." Plant Disease 98, no. 3 (2014): 395–400. http://dx.doi.org/10.1094/pdis-04-13-0433-re.

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Grapevine leafroll ampeloviruses have been recently grouped into two major clades, one for Grapevine leafroll associated virus (GLRaV) 1 and 3 and another one grouping GLRaV-4 and its variants. In order to understand biological factors mediating differential ampelovirus incidences in vineyards, quantitative real-time polymerase chain reactions were performed to assess virus populations in three grapevine varieties in which different infection status were detected: GLRaV-3 + GLRaV-4, GLRaV-3 + GLRaV-4 strain 5, and GLRaV-4 alone. Specific primers based on the RNA-dependent RNA polymerase (RdRp)

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Kuniyuki, Hugo, Jorge Albeto Marques Rezende, José Osmar Gaspar, and Valdir Atsushi Yuki. "Detecção do Grapevine leafroll-associated virus 5 no Estado de São Paulo." Summa Phytopathologica 34, no. 4 (2008): 366–67. http://dx.doi.org/10.1590/s0100-54052008000400014.

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O enrolamento da folha da videira ("grapevine leafroll") é uma doença atribuída a pelo menos nove vírus sorologicamente distintos, Grapevine leafroll-associated viruses 1 a 9 e designados GLRaV-1 a GLRaV-9. No Brasil, já é conhecida a existência do GLRaV-1, GLRaV-2, GLRaV-3 e GLRaV-6. Neste trabalho, foi demonstrada a ocorrência do GLRaV-5 em amostras de videiras cultivadas no Estado de São Paulo, mediante teste de Biotina-ELISA. O vírus foi detectado com baixa incidência nas cultivares avaliadas, exceto na 'Cardinal', que apresentou 100% de infecção. Este é o primeiro relato da ocorrência do

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23

Risovannaya, Valentina, Vitalii Volodin, Yakov Volkov, Elena Stranishevskaya, and Svitlana Goryslavets. "Mixed infecting of grapevine with viruses in the commercial vineyards of the Crimean Peninsula." BIO Web of Conferences 25 (2020): 06005. http://dx.doi.org/10.1051/bioconf/20202506005.

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Viral infections cause the reduction of yields and efficiency of vine cultivation in all viticultural regions of the world. Simultaneous infecting with several viruses, the so-called mixed infection, can significantly increase the severity of symptoms. The article presents the results of study of mixed infecting of grapevine with viruses in the vineyards of the Crimean Peninsula during 2011-2020. More than 800 grapevine samples of 153 varieties selected in 23 vineyards of the Crimea were analyzed over the entire period of study. Presence of phytopathogenic viruses in the samples was determined

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Bendel, Nele, Anna Kicherer, Andreas Backhaus, et al. "Detection of Grapevine Leafroll-Associated Virus 1 and 3 in White and Red Grapevine Cultivars Using Hyperspectral Imaging." Remote Sensing 12, no. 10 (2020): 1693. http://dx.doi.org/10.3390/rs12101693.

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Grapevine leafroll disease (GLD) is considered one of the most widespread grapevine virus diseases, causing severe economic losses worldwide. To date, six grapevine leafroll-associated viruses (GLRaVs) are known as causal agents of the disease, of which GLRaV-1 and -3 induce the strongest symptoms. Due to the lack of efficient curative treatments in the vineyard, identification of infected plants and subsequent uprooting is crucial to reduce the spread of this disease. Ground-based hyperspectral imaging (400–2500 nm) was used in this study in order to identify white and red grapevine plants in

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Reynard, Jean-Sébastien, Pierre H. H. Schneeberger, Jürg Ernst Frey, and Santiago Schaerer. "Biological, Serological, and Molecular Characterization of a Highly Divergent Strain of Grapevine leafroll-associated virus 4 Causing Grapevine Leafroll Disease." Phytopathology® 105, no. 9 (2015): 1262–69. http://dx.doi.org/10.1094/phyto-12-14-0386-r.

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The complete genome sequence of a highly divergent strain of Grapevine leafroll-associated virus 4 (GLRaV-4) was determined using 454 pyrosequencing technology. This virus, designated GLRaV-4 Ob, was detected in Vitis vinifera ‘Otcha bala’ from our grapevine virus collection at Agroscope. The GLRaV-4 Ob genome length and organization share similarities with members of subgroup II in the genus Ampelovirus (family Closteroviridae). Otcha bala was graft-inoculated onto indicator plants of cultivar Gamay to evaluate the biological properties of this new strain, and typical leafroll symptoms were i

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Fuchs, M., T. E. Martinson, G. M. Loeb, and H. C. Hoch. "Survey for the Three Major Leafroll Disease-Associated Viruses in Finger Lakes Vineyards in New York." Plant Disease 93, no. 4 (2009): 395–401. http://dx.doi.org/10.1094/pdis-93-4-0395.

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Vineyards in the Finger Lakes region in New York were surveyed for the three major viruses associated with leafroll disease, i.e., Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2), and Grapevine leafroll-associated virus 3 (GLRaV-3). Target viruses were detected in nearly two-thirds (68%, 65 of 95) of the vineyard blocks surveyed by enzyme-linked immunosorbent assay. Single infections by GLRaV-1, GLRaV-2, and GLRaV-3 occurred in 10% (113 of 1,124), 3% (36 of 1,124), and 15% (173 of 1,124) of the samples tested, respectively, whereas mixed infecti

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Rowhani, Adib, Jerry K. Uyemoto, and Deborah A. Golino. "A Comparison Between Serological and Biological Assays in Detecting Grapevine Leafroll Associated Viruses." Plant Disease 81, no. 7 (1997): 799–801. http://dx.doi.org/10.1094/pdis.1997.81.7.799.

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The efficacy of the serological procedure enzyme-linked immunosorbent assay (ELISA) for detecting grapevine leafroll associated viruses (GLRaV types -1, -2, -3, and -4) was compared with indexing on Vitis vinifera L. cv. Cabernet Franc. Results of the biological assays confirmed the infectious nature of all grapevine sources testing positive by ELISA for GLRaV-1 (9 sources), GLRaV-2 (14 sources), and GLRaV-4 (14 sources), and the noninfectious nature of ELISA-negative grapevines (75 sources). However, among 57 sources testing positive by ELISA for GLRaV-3, or 24 sources with multiple infection

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Akbaş, B., B. Kunter, and D. Ilhan. "Influence of leafroll on local grapevine cultivars in agroecological conditions of Central Anatolia region." Horticultural Science 36, No. 3 (2009): 97–104. http://dx.doi.org/10.17221/27/2008-hortsci.

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Leafroll is one of the most important virus diseases of vineyards of Central Anatolia region. Grapevine leafroll associated viruses GLRaV-1 and GLRaV-3 are the most frequently encountered viruses related with leafroll disease of grapes. The effect of leafroll disease on yield and fruit quality was studied. The disease caused in the average 31.22% decrease of berry weights and 28.72% reduction in total soluble solids, while titratable acidity was increased by 24.39%. The reaction of local cultivars to mixed infections of GLRaV-1 and GLRaV-3 was evaluated and Parmak, Kara dirmit, Kara gevrek and

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Mahfoudhi, N., N. Habili, S. A. Masri, and M. H. Dhouibi. "First Report on the Occurrence of Grapevine leafroll-associated viruses 5 and 9 in Tunisian Grapevines." Plant Disease 91, no. 10 (2007): 1359. http://dx.doi.org/10.1094/pdis-91-10-1359a.

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Grapevine leafroll disease is one of the most important diseases that occurs in cultivated grapevines in the world. So far, nine serologically distinct viruses of the family Closteroviridae have been isolated from diseased vines (3). A previous study (4) has shown that Grapevine leafroll-associated viruses (GLRaV) -1, -2, and -3 are present in Tunisian grapevines and GLRaV-3 is the predominant virus associated with leafroll disease. A survey was conducted in table grapes to identify other viruses associated with this disease. Samples of dormant canes were collected and screened by indirect Bio

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Jones, Taylor, and Mizuho Nita. "Gill's mealybug, Ferrisia gilli, can Transmit Grapevine Leafroll-associated Virus-3 after a 24-hour Acquisition Time." International Journal of Phytopathology 9, no. 2 (2020): 139–44. http://dx.doi.org/10.33687/phytopath.009.02.3385.

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Grapevine leafroll disease (GLD) is a virus disease present in all grapevine-growing regions of the world. Mealybugs and scale insects have been reported as vectors of some grapevine leafroll-associated viruses belongs to the ampeloviruses (family Closteroviridae) in particular with grapevine leafroll-associated virus-1 (GLRaV-1) and GLRaV-3. Both grape (Pseudococcus maritimus) and Gill’s mealybugs are commonly present in Virginia vineyards, but we have limited information on acquisition and transmission of GLRaV-3 by Gill's mealybug (Ferrisia Gilli). We conducted acquisition and transmission

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Fuchs, M., P. Marsella-Herrick, G. M. Loeb, T. E. Martinson, and H. C. Hoch. "Diversity of Ampeloviruses in Mealybug and Soft Scale Vectors and in Grapevine Hosts from Leafroll-Affected Vineyards." Phytopathology® 99, no. 10 (2009): 1177–84. http://dx.doi.org/10.1094/phyto-99-10-1177.

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The occurrence and diversity of Grapevine leafroll-associated virus 1 (GLRaV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) in the soft scales Parthenolecanium corni and Pulvinaria innumerabilis and in the mealybug Pseudococcus maritimus was determined in leafroll-affected vineyards in the Finger Lakes region of New York. Groups of 1 to 4 specimens were collected under loose grapevine bark and tested by reverse-transcription polymerase chain reaction (RT-PCR) for segments of the second diverged copy of the GLRaV-1 coat protein gene or GLRaV-3 heat-shock protein 70-homologue gene. Virus

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FAJARDO, THOR V. M., GILMAR B. KUHN, MARCELO EIRAS, and OSMAR NICKEL. "Detecção de Closterovirus em videira e caracterização parcial de um isolado do Grapevine leafroll-associated virus 3." Fitopatologia Brasileira 27, no. 1 (2002): 58–64. http://dx.doi.org/10.1590/s0100-41582002000100009.

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O enrolamento da folha da videira (Vitis spp.) é uma doença causada por até oito vírus, Grapevine leafroll-associated virus (GLRaV) 1 a 8, sorologicamente distintos e associados ao floema de videiras infetadas. Neste trabalho, foram detectados GLRaV-1 e -3 por DAS-ELISA em 6,9 e 14,7% das amostras analisadas, respectivamente, e provenientes de duas importantes regiões vitícolas do Brasil (Serra Gaúcha e Vale do São Francisco). Os GLRaV-2, -5 e -7 não foram detectados. O GLRaV-3 também foi detectado por dot-ELISA e western blot, observando-se a provável proteína capsidial com cerca de 36 kDa. U

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Soltani, Nourolah, Rongbin Hu, Darrell D. Hensley, David L. Lockwood, Keith L. Perry, and M. R. Hajimorad. "A Survey for Nine Major Viruses of Grapevines in Tennessee Vineyards." Plant Health Progress 21, no. 3 (2020): 157–61. http://dx.doi.org/10.1094/php-03-20-0018-rs.

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Despite the significance of grape production to the fruit industry in Tennessee (TN), no published information has been available on viruses affecting grapevines in the state. Hence, a survey was conducted during the 2016 and 2017 growing seasons to determine the status of nine major viruses of grapevines in TN vineyards by taking advantage of classical serological assays and confirmatory nucleic acid-based diagnostic approaches. A total of 349 samples from 23 grapevine cultivars mostly displaying viral-like symptoms were collected from 23 commercial vineyards. All samples were assayed by DAS-

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Thompson, Brandon D., Jennifer Dahan, Jungmin Lee, Robert R. Martin, and Alexander V. Karasev. "A Novel Genetic Variant of Grapevine leafroll-associated virus-3 (GLRaV-3) from Idaho Grapevines." Plant Disease 103, no. 3 (2019): 509–18. http://dx.doi.org/10.1094/pdis-08-18-1303-re.

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Grapevine leafroll-associated virus-3 (GLRaV-3) is a major constraint on profitable grapevine cultivation. The virus is transmitted efficiently by mealybugs and soft scale insects, or through vegetative propagation by cuttings, and is present worldwide, wherever grapevines are grown. GLRaV-3 exists as a complex of genetic variants currently classified in several phylogenetic groups that can differ from each other by as much as 30% in nucleotide sequence of the whole genome. In the course of the GLRaV-3 testing of wine grapes in southern Idaho, plants of two grapevine cultivars were found to ha

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Porotikova, Elena, Uliana Terehova, Vitalii Volodin, Eugeniya Yurchenko, and Svetlana Vinogradova. "Distribution and Genetic Diversity of Grapevine Viruses in Russia." Plants 10, no. 6 (2021): 1080. http://dx.doi.org/10.3390/plants10061080.

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Viral diseases can seriously damage the vineyard productivity and the quality of grape and wine products. Therefore, the study of the species composition and range of grapevine viruses is important for the development and implementation of strategies and tactics to limit their spread and increase the economic benefits of viticulture. In 2014–2019, we carried out a large-scale phytosanitary monitoring of Russian commercial vineyards in the Krasnodar region, Stavropol region and Republic of Crimea. A total of 1857 samples were collected and tested for the presence of Grapevine rupestris stem pit

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Tomazic, Irma, Zora Korosec-Koruza, and Natasa Petrovic. "Sanitary status of slovenian indigenous grapevine cultivar Refosk." OENO One 39, no. 1 (2005): 19. http://dx.doi.org/10.20870/oeno-one.2005.39.1.908.

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<p style="text-align: justify;">Sanitary status of visually selected and nonselected indigenous grapevine cultivar 'Refosk' (Vitis vinifera L. cv. 'Refosk') was evaluated. Nine viruses were tested by ELISA: Nepoviruses Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV), Closteroviruses Grapevine leafroll associated viruses 1 (GLRaV-1), 2 (GLRaV-2), 3 (GLRaV-3), and 6 (GLRaV-6), Grapevine fleck virus (GFkV) and Vitiviruses Grapevine virus A (GVA) and Grapevine virus B (GVB). All viruses except ArMV and GVB were detected. The vines of cv. ‘Refosk’ passing the visual selection we

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Jarugula, Sridhar, Olufemi J. Alabi, Robert R. Martin, and Rayapati A. Naidu. "Genetic Variability of Natural Populations of Grapevine leafroll-associated virus 2 in Pacific Northwest Vineyards." Phytopathology® 100, no. 7 (2010): 698–707. http://dx.doi.org/10.1094/phyto-100-7-0698.

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Genetic variability of field populations of Grapevine leafroll-associated virus 2 (GLRaV-2) in Pacific Northwest (PNW) vineyards was characterized by sequencing the entire coat protein (CP) and a portion of the heat-shock protein-70 homolog (HSP70h) genes. Phylogenetic analysis of CP and HSP70h nucleotide sequences obtained in this study and corresponding sequences from GenBank revealed segregation of GLRaV-2 isolates into six lineages with virus isolates from PNW distributed in ‘PN’, ‘H4’, and ‘RG’ lineages. An estimation of the ratio of nonsynonymous substitutions per nonsynonymous site to s

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Adiputra, Jati, Sandya R. Kesoju, and Rayapati A. Naidu. "The Relative Occurrence of Grapevine leafroll-associated virus 3 and Grapevine red blotch virus in Washington State Vineyards." Plant Disease 102, no. 11 (2018): 2129–35. http://dx.doi.org/10.1094/pdis-12-17-1962-re.

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Vineyard surveys were conducted for three consecutive seasons in eastern Washington State, the major grapevine-growing region in the state, to document the occurrence of Grapevine leafroll-associated virus 3 (GLRaV-3) and Grapevine red blotch virus (GRBV). The majority of samples were collected from red-berried wine grape (Vitis vinifera) cultivars exhibiting symptoms of or suspected for grapevine leafroll (GLD) and red blotch (GRBD) diseases. A limited number of samples from white-berried cultivars were collected randomly due to the lack of visual symptoms. Samples were collected from a total

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Kumar, S., S. D. Sawant, I. S. Sawant, K. Prabha, R. K. Jain, and V. K. Baranwal. "First Report of Grapevine leafroll-associated virus 1 Infecting Grapevines in India." Plant Disease 96, no. 12 (2012): 1828. http://dx.doi.org/10.1094/pdis-07-12-0647-pdn.

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Viticulture, one of the most remunerative farming enterprises of India, is seriously affected by leafroll disease, which accounts for 62% of the losses in grape production worldwide due to viral diseases (4). Grapevine leafroll-associated virus 3 and 1 (GLRaV-3 and GLRaV-1) of the family Closteroviridae are the two most common viruses associated with the leafroll disease of grapevine (1). GLRaV-3 was previously confirmed in India through RT-PCR, cloning, and sequencing (2). A survey was conducted during 2010 and 2011 in the Nashik and Pune regions of western India and reddening of interveinal

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Mahfoudhi, N., M. Digiaro, and M. H. Dhouibi. "Transmission of Grapevine Leafroll Viruses by Planococcus ficus (Hemiptera: Pseudococcidae) and Ceroplastes rusci (Hemiptera: Coccidae)." Plant Disease 93, no. 10 (2009): 999–1002. http://dx.doi.org/10.1094/pdis-93-10-0999.

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Grapevine leafroll associated virus-3 (GLRaV-3) and Grapevine leafroll associated virus-5 (GLRaV-5), two members of the genus Ampelovirus associated with grapevine leafroll disease, were transmitted by the mealybug Planococcus ficus and the soft scale insect Ceroplastes rusci from infected to healthy vines under experimental conditions. The efficiencies of transmission of GLRaV-3 and GLRaV-5 by P. ficus were 23.3 and 8.3%, respectively, and by C. rusci were 3.3 and 1.7%, respectively. Juvenile instars of P. ficus were more efficient in transmission of the viruses than adult females. This is th

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Cabaleiro, C., and A. Segura. "Field Transmission of Grapevine Leafroll Associated Virus 3 (GLRaV-3) by the Mealybug Planococcus citri." Plant Disease 81, no. 3 (1997): 283–87. http://dx.doi.org/10.1094/pdis.1997.81.3.283.

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The spatial distributions of grape plants with symptoms of grapevine leafroll in nine vineyards in the Galician wine-growing region Rías Baixas (northwestern Spain) were analyzed. In five, significant aggregations of diseased plants and/or disease gradients toward one of the borders of the plots constituted indirect evidence of vector transmission by a nonflying vector. From 1991 to 1995, vines from two vineyards were tested for grapevine leafroll associated virus 3 (GLRaV-3) by enzyme-linked immunosorbent assay. In one of these vineyards, which had been virus-free when planted, GLRaV-3 was de

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Komínek, P. "Selection of RNA isolation method for molecular detection of grapevine viruses." Plant Protection Science 38, SI 2 - 6th Conf EFPP 2002 (2017): 267–70. http://dx.doi.org/10.17221/10463-pps.

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Grapevines infected with Grapevine leafroll-associated virus-1 (GLRaV-1) and Grapevine leafroll-associated virus-3 (GLRaV-3) were selected. Total RNA was isolated from grapevine phloem tissue scrapped from dormant canes by three different methods: extraction with urea buffer followed with phenol-chloroform extraction, method using Concert<sup>TM</sup> reagent (Invitrogen) followed with chloroform-isopropylalcohol extraction, and procedure using RNeasy Plant Mini Kit (Qiagen). The highest yield of RNA was obtained using Concert<sup>TM</sup> reagent. If this RNA was used

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Vončina, Darko, Maher Al Rwahnih, Adib Rowhani, Mona Gouran, and Rodrigo P. P. Almeida. "Viral Diversity in Autochthonous Croatian Grapevine Cultivars." Plant Disease 101, no. 7 (2017): 1230–35. http://dx.doi.org/10.1094/pdis-10-16-1543-re.

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A survey was conducted on nine autochthonous grapevine cultivars grown along the Croatian coastal region. In total, 48 vines (44 from germplasm collection, 4 from vineyards) originating from 23 sites were tested for 26 viruses using molecular methods. Results revealed high infection rates with Grapevine leafroll-associated virus 3 (GLRaV-3); Grapevine virus A (GVA, both 91.7%); Grapevine fleck virus (GFkV, 87.5%); and Grapevine rupestris stem pitting-associated virus (GRSPaV, 83.3%). Other detected viruses were: Grapevine fanleaf virus (GFLV); Grapevine leafroll-associated viruses 1, 2, and st

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Hančević, Katarina, Pasquale Saldarelli, Mate Čarija, et al. "Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia." Plants 10, no. 1 (2020): 17. http://dx.doi.org/10.3390/plants10010017.

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Sixteen grapevine cultivars from Mediterranean Croatia were surveyed for the presence of 10 of the most economically important grapevine viruses. The presence of Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Grapevine leafroll associated virus-1, -2, and -3 (GLRaV-1; GLRaV-2 and GLRaV-3), Grapevine virus A (GVA) and B (GVB), Grapevine fleck virus (GFkV), Grapevine rupestris stem pitting associated virus (GRSPaV), and Grapevine Pinot gris virus (GPGV) were tested by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). All 71 analy

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Lyu, M. D., M. J. Li, J. Li, X. M. Li, and Y. Q. Cheng. "First Report of Grapevine leafroll-associated virus 7 in Two Native Grape Varieties in China." Plant Disease 97, no. 1 (2013): 150. http://dx.doi.org/10.1094/pdis-08-12-0760-pdn.

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Grapevine leafroll disease (GLD) is one of the most economically important diseases of cultivated grapevines (Vitis vinifera), causing decrease in yield, as well as decreasing the sugar levels and increasing the acidity of the berries (1). There are currently at least 10 serologically distinct viruses, referred to as grapevine leafroll-associated viruses (GLRaVs), from the family Closteroviridae that are associated with leafroll disease (4). China is one of the world's leading grape producers, and nearly 75% of the vineyards in China are located in Xinjiang Uygur Autonomous Region, and Hebei,

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Lunden, S., and W. Qiu. "First Report of Grapevine leafroll-associated virus 2 in a Hybrid Grape ‘Vidal Blanc’ in Missouri." Plant Disease 96, no. 3 (2012): 462. http://dx.doi.org/10.1094/pdis-10-11-0834.

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Grapevine leaf roll disease (GLRD) is one of the most prevalent viral diseases in vineyards worldwide. At least 10 Grapevine leafroll-associated viruses (GLRaV-1 to -7, and -9, -10, and -11) are associated with GLRD. GLRaV-2 has a number of distinct isolates that are associated not only with GLRD, but also with graft incompatibility, young vine decline, and rootstock stem lesions. In the United States, GLRaV-2 isolates have been reported in the states of California (4), New York (1), Oregon, and Washington (3). A survey of grapevine viruses was conducted on seven grape cultivars, Norton, Chamb

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Fajardo, Thor V. M., Marcelo Eiras, Paula G. Schenato, Osmar Nickel, and Gilmar B. Kuhn. "Avaliação da variabilidade do Grapevine leafroll-associated virus 1 e 3 por análise de seqüências de nucleotídeos e polimorfismo conformacional de fita simples." Fitopatologia Brasileira 30, no. 2 (2005): 177–82. http://dx.doi.org/10.1590/s0100-41582005000200013.

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As principais espécies de vírus envolvidas na etiologia do enrolamento da folha da videira (Vitis spp.) são Grapevine leafroll-associated virus 1 e 3 (GLRaV-1 e -3). Neste estudo da variabilidade desses vírus, foram amplificados dois fragmentos de DNA (396 bp do GLRaV-1 e 602 bp do GLRaV-3) por RT-PCR, a partir de RNA total extraído de nervuras e pecíolos de videiras infetadas, utilizando-se dois pares de oligonucleotídeos. Os DNAs amplificados foram clonados e reamplificados, a partir dos clones recombinantes, e comparados quanto às diferenças conformacionais das fitas simples desnaturadas (S

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Martin, R. R., K. C. Eastwell, A. Wagner, S. Lamprecht, and I. E. Tzanetakis. "Survey for Viruses of Grapevine in Oregon and Washington." Plant Disease 89, no. 7 (2005): 763–66. http://dx.doi.org/10.1094/pd-89-0763.

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Grapevines (Vitis spp.) in Washington and Oregon were surveyed for the prevalence of key grapevine viruses. Samples collected from 1,522 vines in Washington were tested for Rupestris stem pitting associated virus (RSPaV), Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Tomato ringspot virus (ToRSV), and Grapevine leafroll associated virus-3 (GLRaV-3). Tests were also conducted for GLRaV-1 and -2 on 420 samples from Washington. Two hundred forty samples collected from wine grape vineyards in Oregon were tested for GLRaV-1, -2, and -3, and an additional 2,880 samples were collected f

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Xiao, Huogen, Caihong Li, Maher Al Rwahnih, Valerian Dolja, and Baozhong Meng. "Metagenomic Analysis of Riesling Grapevine Reveals a Complex Virome Including Two New and Divergent Variants of Grapevine leafroll-associated virus 3." Plant Disease 103, no. 6 (2019): 1275–85. http://dx.doi.org/10.1094/pdis-09-18-1503-re.

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The virome of a major white wine grape of cultivar Riesling showing decline and leafroll disease symptoms was analyzed through high-throughput sequencing (HTS) using total RNAs as templates and the Illumina HiSeq 2500 platform. Analysis of HTS data revealed the presence of five viruses and three viroids in the infected vine. These viruses are Grapevine leafroll-associated virus 1 (GLRaV-1) and GLRaV-3 (genus Ampelovirus, family Closteroviridae) and three viruses of the family Betaflexiviridae (namely, Grapevine virus A [GVA], Grapevine virus B, and Grapevine rupestris stem pitting-associated v

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Crnogorac, Ana, Stefano Panno, Ana Mandić, et al. "Survey of five major grapevine viruses infecting Blatina and Žilavka cultivars in Bosnia and Herzegovina." PLOS ONE 16, no. 1 (2021): e0245959. http://dx.doi.org/10.1371/journal.pone.0245959.

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The sanitary status of grapevines has not yet been considered sufficiently in vineyards throughout Bosnia and Herzegovina (BiH). An extensive survey of five major grapevine viruses in the country was carried out in 2019. A total of 630 samples from the two dominant autochthonous cultivars, named Žilavka and Blatina, were tested by DAS-ELISA for the presence of grapevine leafroll-associated viruses (GLRaV-1 and 3), grapevine fleck virus (GFkV), grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). Eighty-eight % of the samples were positive for at least one virus, and all five viruses

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