Dissertations / Theses on the topic 'Gluconobacter'
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McKibben, Laura Ann. "Characterization of plasmids in Gluconobacter /." This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-08142009-040503/.
Full textMcKibben, Ann Laura. "Characterization of plasmids in Gluconobacter." Thesis, Virginia Tech, 1992. http://hdl.handle.net/10919/44232.
Full textVan, Wyk Nathan. "Analysis of dextrin dextranase from Gluconobacter oxydans." Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/2619.
Full textDextran is a high value glucose polymer used in medicine and an array of laboratory techniques. It is synthesised by lactic-acid bacteria from sucrose but has also reportedly been produced by Gluconobacter oxydans (G. oxydans) from a range of maltooligosaccharides (MOS) via the action of dextrin dextranase (DDase). In this study the presence of DDase is investigated in two G. oxydans strains (ATCC 621H and ATCC 19357) and shown to be present in the ATCC 19357 strain, but not in the ATCC 621H strain. The enzyme was partially purified from the ATCC 19357 strain, and its kinetic properties investigated. The partially purified protein was also digested with trypsin, and de novo peptide sequences obtained from it. Several attempts were made to obtain the gene coding for the DDase. These include amplifying an open reading frame from the G. oxydans genome coding for a glycosyltransferase with the approximate molecular weight of the DDase, using the peptide sequences obtained from the partially purified protein to design degenerate PCR primers and the production of a genomic DNA library for functional screening in E. coli. None of these approaches led to the successful isolation of the extracellular DDase sequence.
Burnley, Leigh-Emma. "Heavy Metal Resistance in the Genus Gluconobacter." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/35993.
Full textMaster of Science
Prust, Christina. "Entschlüsselung des Genoms von Gluconobacter oxydans 621H - einem Bakterium von industriellem Interesse." [S.l.] : [s.n.], 2004. http://webdoc.sub.gwdg.de/diss/2004/prust/prust.pdf.
Full textHoffmeister, Marc. "Untersuchungen zur Physiologie des Essigsäurebakteriums Gluconobacter oxydans 621H." [S.l.] : [s.n.], 2006. http://webdoc.sub.gwdg.de/diss/2006/hoffmeister.
Full textSwartwood, Suzanne Christine. "The evolution of hydrogen sulfide by Gluconobacter species." Thesis, This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-02132009-171359/.
Full textEdwards, Deborah Elizabeth. "Diversity of limited oxidations accomplished by gluconobacter oxydans." Thesis, Virginia Tech, 1990. http://hdl.handle.net/10919/42065.
Full textGluconobacter oxydans is characterized by the ability to carry out rapid, single-step oxidations of many different hydroxyl-containing compounds. These oxidations are believed to be catalyzed by the membrane-bound NAD(P)-independent dehydrogenases. Experiments were designed to use G. oxydans ATCC strain 621 to determine the contribution of these dehydrogenases in whole-cell oxidations and to determine the range of substrates that can be oxidized by the membrane fraction of these cells when grown on a single substrate. My first hypothesis was that the membranes would accomplish these oxidations at the same rate as an equivalent number of whole cells. Oxidative activity data obtained from using both oxygen uptake and tetranitroblue tetrazolium assays, however, did not support this hypothesis. I attribute this to the probability that the membranes were damaged during isolation of the membrane fraction and, therefore, were unable to exhibit full oxidative potential. My second hypothesis was that the membranes from cells grown on one substrate would oxidize many other substrates. Potassium fenicyanide was used to assay the oxidative activity of the membrane fraction of cells grown on glycerol. Of 41 substrates tested all were significantly oxidized. I concluded from these data, therefore, that the enzyme(s) responsible for the oxidation of these substrates are synthesized constitutively. Unfortunately, one cannot draw any conclusions as to whether or not these enzymes are highly substrate-specific. I speculate that one or a few enzymes show a broad range of substrate specificity, as it would be inefficient for the cell to consecutively synthesize more than forty different substrate-specific enzymes for substrates it may never encounter.
Master of Science
Pontes, Simone Gomes. "Produção de Dihidroxiacetona por células de Gluconobacter Oxydans a partir do Glicerol." Niterói, 2017. https://app.uff.br/riuff/handle/1/3400.
Full textMade available in DSpace on 2017-04-19T16:57:00Z (GMT). No. of bitstreams: 1 Pontes, Simone Gomes [Dissertação, 2012].pdf: 1302242 bytes, checksum: 7aab3cecd1b771d41f103c491d846579 (MD5)
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A dihidroxiacetona (DHA) é uma molécula constituída por três carbonos e não tóxica, utilizada como insumo para as indústrias de cosméticos, fármacos e química fina. É produzida industrialmente por fermentação, utilizando a bactéria Gluconobacter oxydans. Esse processo tem como principal limitação a inibição do crescimento tanto pelo substrato – glicerol – quanto pelo produto – DHA e, por tal, estudos recentes descrevem propostas para melhoria do processo. Sendo a conversão de glicerol a DHA realizada por uma única enzima em uma etapa, o presente trabalho considera que tal processo se enquadra nas definições de uma biotransformação, ou seja, a utilização de um catalisador biológico com o propósito de converter um substrato a um produto estruturalmente similar, através de modificações específicas e utilizando um número limitado de etapas enzimáticas. Dessa forma, neste estudo foram avaliados comparativamente a secagem de células em acetona e, em um segundo momento, a utilização de células de Gluconobacter oxydans previamente crescidas, para a produção de DHA a partir de glicerol. Objetivando contornar o principal problema do processo, que é a inibição do crescimento microbiano pelo substrato e pelo produto, foram testadas duas linhagens. A utilização de células secas em acetona se mostrou possível, porém os resultados não foram reprodutíveis e células previamente crescidas por 24 horas passaram a ser usadas nos experimentos de biotransformação. O pH e a temperatura de reação foram selecionados a partir de um planejamento delineamento composto central rotacional como sendo de 34ºC e pH de 4,5, para G. oxydans CCT 0552 e de 26ºC e pH de 4,5 para G. oxydans CCT 0174. A linhagem G. oxydans CCT 0552 se mostrou mais adequada à oxidação de glicerol à DHA, com aumento do acúmulo de DHA no meio reacional com o tempo (2,1 g/g biomassa) e com a produtividade constante (0,45 g/g biomassa). Foi constatada perda de atividade nas células estocadas por congelamento, o que leva à necessidade de selecionar um melhor método de conservação das células para a utilização na produção
The dihydroxyacetone (DHA) is a non-toxic molecule consisting of three carbons, used in the cosmetics, pharmaceuticals and fine chemicals industry. The DHA is industrially produced by fermentation, using the bacteria Gluconobacter oxydans. The main bottleneck of this process is the growth inhibition by the substrate – glycerol – and the product – DHA. This problem leads recent studies to describe proposals for improving the process. As the conversion of glycerol to DHA is performed by a single enzyme in one step, this study considers that this process fits in the definitions of biotransformation, in other words, the use of a biological catalyst in order to convert a substrate for a structurally similar products, by speficic modifications, and using a limited number of enzymatic steps. Thus, this study were assessed by comparison with drying of cells in acetone and in second stage, the use of previously grown cells of Gluconobacter oxydans for the production of DHA from glycerol. The use of dried cells proved to be possible, but the results were not reproducible and the biotransformation experiments were done with previously grown cells of 24 hours age. . The best pH and temperature for the reaction were selected from a central composite design as being 34o C and pH 4.5 for G. oxydans CCT 0552 and 26o C and pH 4.5 for G. oxydans CCT 0174. The strain G. oxydans CCT 0552 was more suitable for the oxidation of glycerol to DHA, with increased accumulation of DHA in the reaction media (2,1 g/g biomass) and constant productivity (0,45 g/g biomass). Loss of activity was observed in cells stored by freezing, which leads to the need to select a best method of preserving cells for the production
Brookman, Lori L. "Characterization of plasmids among the three species of Gluconobacter." Diss., This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-06062008-170132/.
Full textKosciow, Konrad [Verfasser]. "Untersuchungen zur Erweiterung des Substratspektrums von Gluconobacter oxydans / Konrad Kosciow." Bonn : Universitäts- und Landesbibliothek Bonn, 2018. http://d-nb.info/1188732609/34.
Full textKallnik, Verena [Verfasser]. "Untersuchungen zur Polyoloxidation in Gluconobacter oxydans und Thermotoga maritima / Verena Kallnik." Bonn : Universitäts- und Landesbibliothek Bonn, 2013. http://d-nb.info/1044971584/34.
Full textMalherbe, Christiaan Johannes. "Control analysis of mixed populations of gluconobacter oxydans and saccharomyces cerevisiae." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5396.
Full textENGLISH ABSTRACT: In the last decade a need arose to find a theoretical framework capable of gaining a quantitative understanding of ecosystems. Control analysis was proposed as a suitable candidate for the analysis of ecosystems with various theoretical applications being developed, i.e. trophic control analysis (TCA) and ecological control analysis (ECA). We set out to test the latter approach through experimental means by applying techniques akin to enzyme kinetics of biochemistry on a simple ecosystem between Saccharomyces cerevisiae and Gluconobacter oxydans. However, this exercise was far more complex than we originally expected due to the extra metabolic activities presented by both organisms. Nevertheless, we derived suitable kinetic equations to describe the metabolic behaviour of both organisms, with regards to the activities of interest to us, from pure culture experiments. We developed new techniques to determine ethanol and oxygen sensitivity of G. oxydans based on its obligately aerobic nature. These parameters were then used to build a simple kinetic model and a more complex model incorporating oxygen limited metabolism we observed at higher cell densities of G. oxydans. Our models could predict both situations satisfactorily for pure cultures and especially the more complex model could describe the lack of linearity observed between metabolic activity and cell density at higher cell densities of G. oxydans. Mixed populations of S. cerevisiae and G. oxydans reached quasi-steady states in terms of ethanol concentration and acetate flux, which was a positive indication for the application of control analysis on the ecosystem. However, the theoretical models based on parameters derived from pure culture experiments did not predict mixed culture steady states accurately. Careful analysis showed that these parameters were mostly under-estimated for G. oxydans and overestimated for S. cerevisiae. Hence, we calculated the kinetic parameters for mixed population assays directly from the experimental data obtained from mixed cultures. We could calculate the control coefficients directly from the experimental data of mixed population studies and compare it with those from theoretical models based on 3 different parameter sets. Our analysis showed that the yeast had all the control over the acetate flux while control over the steady-state ethanol was shared. The strength of our approach lies in designing our experiments with a control analysis approach in mind, but we have also shown that even for simple ecosystems this approach is non-trivial. Despite the various experimental challenges, this approach was very rewarding due to the extra information obtained especially regarding control structure with regards to the steady-state ethanol concentration.
AFRIKAANSE OPSOMMING: In die afgelope dekade het daar ’n behoefte ontstaan na ‘n teoretiese raamwerk om tot ‘n kwantitatiewe begrip van ekosisteme te kom. As kandidaat vir so tipe raamwerk is kontrole analise voorgestel gepaardgaande met die ontwikkeling van verskeie teoretiese toepassings, i.e. trofiese kontrole analise en ekologiese kontrole analise. In hierdie tesis het ons laasgenoemde aanslag eksperimenteel ondersoek op ‘n eenvoudige ekosisteem, tussen Saccharomyces cerevisiae en Gluconobacter oxydans, deur gebruik te maak van tegnieke vanuit ensiemkinetika van biochemie. Hierdie strategie was egter baie meer kompleks as wat oorspronklik verwag is as gevolg van verdere metabolise aktiwiteite aanwesig in beide organismes. Ons het egter steeds daarin geslaag om kinetiese vergelykings af te lei, vanuit suiwer kulture, wat die metaboliese gedrag van beide organismes beskryf vir die aktiwiteite van belang vir ons studie. Ons het nuwe tegnieke, gebaseer op die aerobiese natuur van G. oxydans, ontwikkel om die sensitiwiteit van G. oxydans vir etanol en suurstof te bepaal. Hierdie parameters is gebruik om eers ’n eenvoudige model en toe ‘n meer gevorderde model, wat die suurstof-beperkte metabolisme van G. oxydans by hoër biomassa te beskryf, op te stel. Beide modelle was baie effektief in die voorspelling van die situasies waarvoor hulle ontwikkel is vir die suiwer kulture waar veral die meer gevorderde model die gebrek aan ‘n linieêre verband tussen die metabolisme van G. oxydans en biomassa by hoër biomassa kon beskryf. ’n Bemoedigende aanduiding dat kontrole analise toegepas kon word op die ekosisteem was dat mengkulture van S. cerevisiae en G. oxydans het quasi-bestendige toestande bereik het in terme van etanol konsentrasies en asetaat-fluksie. Die teoretiese modelle gebaseer op die parameters afgelei vanaf suiwer kulture kon egter nie die bestendige toestande in mengkulture akkuraat voorspel nie. Nadere ondersoek het aangedui dat die parameters meesal onderskat is vir G. oxydans en oorskat is vir S. cerevisiae. Gevolglik het ons die kinetiese parameters vir mengkulture direk van eksperimentele data van die mengkulture bereken. Verder kon ons die kontrole koeffisiente ook direk vanaf die eksperimentele data van mengkulture bereken en vergelyk met dié bereken vanuit die teoretiese modelle gebaseer op drie verskillende paremeter-stelle. Ons analise het gewys dat die gis alle beheer op die asetaat-fluksie uitoefen en dat die beheer oor die etanol-konsnetrasie gedeel is tussen die twee organismes. Die krag van ons aanslag lê daarin dat die eksperimente ontwerp is met ‘n kontrole analise in gedagte, maar ons het ook bewys dat hierdie aanslag selfs vir eenvoudige ekosisteme nie triviaal is nie. Ten spyte van die eksperimentele uitdagings, was die aanslag baie waardevol as gevolg van die ekstra inligting verkry met spesifieke klem op die kontrole-struktuur met betrekking tot die etanol konsentrasie by bestendige toestand.
Koslik, Heike. "Herstellung und Eigenschaften der Fructosyltransferasen aus Rahnella aquatilis ATCC 33071 und Gluconobacter cerinus." Clausthal-Zellerfeld Papierflieger, 2009. http://d-nb.info/997006889/04.
Full textZahid, Nageena [Verfasser]. "Osmotic stress response in the industrially important bacterium Gluconobacter oxydans / Nageena Zahid." Bonn : Universitäts- und Landesbibliothek Bonn, 2017. http://d-nb.info/1130704564/34.
Full textNassif, Lana Amine. "The Production of 2-Keto-L-Gulonic Acid by Different Gluconobacter Strains." Thesis, Virginia Tech, 1997. http://hdl.handle.net/10919/30590.
Full textMaster of Science
Sainz, Perez Florencia. "Selection and optimization of acetic acid bacteria for d-gluconic acid production." Doctoral thesis, Universitat Rovira i Virgili, 2016. http://hdl.handle.net/10803/401541.
Full textEn esta tesis doctoral, se realizó la selección de bacterias acéticas (BA) para llevar a cabo una fermentación selectiva de D-glucosa en ácido D-glucónico, sin consumo de fructosa. Se seleccionaron tres cepas de BA, dos de ellas pertenecientes al género Gluconobacter y otra del género Acetobacter, siendo las cepas de Gluconobacter las mejor preparadas para esta oxidación. Se midió la actividad de las deshidrogenasas de membrana que participan en la oxidación completa de la D-glucosa. Además, se secuenciaron los genes que codifican para estas enzimas y se utilizaron para construir árboles filogenéticos, obteniendo como resultado que los géneros Acetobacter y Gluconobacter quedan separados en diferentes grupos. También se estudiaron los requerimientos nutricionales de las BA, centrándose principalmente en la utilización de nitrógeno. El crecimiento de estas bacterias fue mejor en los medios con una combinación completa de aminoácidos y de amonio. Las cepas seleccionadas se secuenciaron para mejorar los protocolos de seguimiento de estas bacterias durante el proceso
In this PhD thesis, the selection of Acetic acid bacteria (AAB) was carried out to perform a selective fermentation of D-glucose into D-gluconic acid, without consuming fructose. Three AAB strains were selected, two of them belonging to the Gluconobacter genus and another to Acetobacter, being the Gluconobacter strains better prepared for this oxidation. The activities of the membrane-bound dehydrogenases involved in the complete oxidation of D-glucose were measured. Furthermore, the genes encoding for these enzymes were sequenced and used to construct phylogenetic trees, obtaining as a result that Acetobacter and Gluconobacter genera grouped into different clusters. The nutritional requirements, mainly focused on nitrogen, for AAB were also studied. The growth of these bacteria was better in media with a complete combination of amino acids and ammonium. The selected strains were sequenced to improve the monitoring protocols for these bacteria in the process.
Bremus, Christoph. "Untersuchungen zur Bildung der Vitamin C-Vorstufe 2-Keto-L-Gulonsäure mit Gluconobacter oxydans." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=982171625.
Full textMacauley-Patrick, Susan E. "Physiological studies on the biotransformation of D-sorbitol to L-sorbose by 'Gluconobacter suboxydans'." Thesis, University of Strathclyde, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288751.
Full textAnriany, Yuda Adha. "The nature of sorbital (a primary) and sorbose (a secondary) dehydrogenases of Gluconobacter species." Thesis, This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-06082009-170814/.
Full textBoerman, Patrice Anne. "The role of respiration-dependent proton translocation in the acid tolerance of Gluconobacter oxydans." Thesis, Virginia Tech, 1988. http://hdl.handle.net/10919/43835.
Full textMaster of Science
Oliveira, Carolina Corado da Silva. "An?lise comparativa dos genes de reparo do DNA em Gluconacetobacter diazotrophicus e Gluconobacter oxydans." Universidade Federal do Rio Grande do Norte, 2009. http://repositorio.ufrn.br:8080/jspui/handle/123456789/16765.
Full textGluconacetobacter diazotrophicus ? uma alfa-proteobact?ria Gram-negativa, tolerante a meios ?cidos, fixadora de nitrog?nio atmosf?rico e foi a primeira bact?ria diazotr?fica endof?tica isolada da cana-de-a??car. Por sua vez, Gluconobacter oxydans, tamb?m alfa-proteobact?ria Gram-negativa, possui a capacidade de oxidar incompletamente alco?is e carboidratos. Ambas de interesse biotecnol?gico e industrial, essas bact?rias tiveram seus genomas seq?enciados completamente em 2007. Desta forma, foi de interesse desse trabalho analisar e comparar os genes de reparo do DNA devido sua import?ncia na manuten??o da integridade gen?mica. Sendo assim, as vias de reparo presentes nos dois organismos foram identificadas, utilizando como base uma terceira alfa-proteobact?ria, a Caulobacter crescentus, cujos genes de reparo foram descritos por um trabalho anterior e tamb?m os genes bem estabelecidos para o reparo do DNA em Escherichia coli. Para esse estudo, um banco de dados contendo ort?logos para os genes de reparo de DNA encontrados nos organismos foi criado e an?lises comparativas por similaridade usando o pacote Blast e o software Clustal foram feitas. Este estudo demonstrou que as principais vias de reparo ao DNA reparos por excis?o, reparo direto, reparo recombinacional e reparo pelo sistema SOS est?o presentes nos organismos analisados, demonstrando, na maioria das vezes, boa similaridade com E. coli. Interessantemente, foram encontradas duplica??es g?nicas nos quais uma das c?pias estava presente no cromossomo e a outra, no plasm?deo, como no caso de UvrD, DnaE e Ssb, possivelmente caracterizando eventos de transfer?ncia lateral. Por fim, uma grande novidade foi a identifica??o de ort?logos para RecB em G. diazotrophicus e G. oxydans e de ort?logos duplicados de RecD em G. diazotrophicus. At? o momento, n?o havia sido relatada a presen?a de membros da via de inicia??o RecBCD do reparo recombinacional em alfaproteobact?rias
Munaretto, Francesco. "Evaluation of microencapsulated silicone oils as oxygen carriers in the production of dihydroxyacetone by Gluconobacter oxydans." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=24067.
Full textHoffmann, Juliane J. [Verfasser]. "Etablierung und Optimierung eines Produktionssystems zur Synthese des alternativen Süßstoffs 5-Ketofruktose in Gluconobacter-Stämmen / Juliane J. Hoffmann." Bonn : Universitäts- und Landesbibliothek Bonn, 2021. http://d-nb.info/1238687474/34.
Full textPeriadnadi. "Vorkommen und Stoffwechselleistungen von Bakterien der Gattungen Acetobacter und Gluconobacter während der Weinbereitung unter Berücksichtigung des Zucker-Säure-Stoffwechsels." [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=96992965X.
Full textSiemen, Anna [Verfasser]. "Identifizierung und Charakterisierung neuartiger Oxidoreduktasen in Gluconobacter oxydans und Produktion des potentiellen Süßstoffes 5-Keto-D-Fruktose / Anna Siemen." Bonn : Universitäts- und Landesbibliothek Bonn, 2018. http://d-nb.info/1160594457/34.
Full textBurger, Christian [Verfasser], Dirk [Akademischer Betreuer] Weuster-Botz, Wolfgang [Gutachter] Liebl, and Dirk [Gutachter] Weuster-Botz. "Ganzzellbiokatalyse mit rekombinanten Gluconobacter oxydans Stämmen / Christian Burger ; Gutachter: Wolfgang Liebl, Dirk Weuster-Botz ; Betreuer: Dirk Weuster-Botz." München : Universitätsbibliothek der TU München, 2019. http://d-nb.info/1196293104/34.
Full textVanLare, Ian Judd. "Isolation and characterization of the (NAD(P)-independent)polyol dehydrogenase from the plasma-membranes of gluconobacter oxydans ATCC strain 621." Diss., Virginia Tech, 1997. http://hdl.handle.net/10919/40249.
Full textJunker, Anja [Verfasser], Wolfgang [Akademischer Betreuer] Liebl, and Matthias A. [Akademischer Betreuer] Ehrmann. "Untersuchung des Zentralstoffwechsels von Gluconobacter oxydans durch die Etablierung eines markerfreien Deletionssystems / Anja Junker. Gutachter: Matthias A. Ehrmann. Betreuer: Wolfgang Liebl." München : Universitätsbibliothek der TU München, 2012. http://d-nb.info/1021499064/34.
Full textHerweg, Elena Ruth [Verfasser], Jochen [Akademischer Betreuer] Büchs, and Uwe [Akademischer Betreuer] Deppenmeier. "Characterization of Gluconobacter oxydans strains and development of a production process for 5-Ketofructose / Elena Ruth Herweg ; Jochen Büchs, Uwe Deppenmeier." Aachen : Universitätsbibliothek der RWTH Aachen, 2019. http://d-nb.info/1192308727/34.
Full textOstermann, Steffen Verfasser], Marco [Akademischer Betreuer] [Oldiges, Lars Mathias [Akademischer Betreuer] Blank, and Miriam [Akademischer Betreuer] Rosenbaum. "Globale und lokale Untersuchung des Stoffwechsels von Gluconobacter oxydans mit Hilfe von Isotopen / Steffen Ostermann ; Marco Oldiges, Lars Mathias Blank, Miriam Agler-Rosenbaum." Aachen : Universitätsbibliothek der RWTH Aachen, 2016. http://d-nb.info/1130590402/34.
Full textOstermann, Steffen [Verfasser], Marco [Akademischer Betreuer] Oldiges, Lars Mathias [Akademischer Betreuer] Blank, and Miriam [Akademischer Betreuer] Rosenbaum. "Globale und lokale Untersuchung des Stoffwechsels von Gluconobacter oxydans mit Hilfe von Isotopen / Steffen Ostermann ; Marco Oldiges, Lars Mathias Blank, Miriam Agler-Rosenbaum." Aachen : Universitätsbibliothek der RWTH Aachen, 2016. http://d-nb.info/1130590402/34.
Full textKranz, Angela [Verfasser], Michael [Akademischer Betreuer] Bott, and Michael [Gutachter] Bott. "High-resolution genome and transcriptome analysis of Gluconobacter oxydans 621H and growth-improved strains by next-generation sequencing / Angela Kranz ; Gutachter: Michael Bott ; Betreuer: Michael Bott." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2018. http://d-nb.info/1169393349/34.
Full textKiefler, Ines Verfasser], Michael [Akademischer Betreuer] [Gutachter] [Bott, and Ulrich [Gutachter] Schulte. "Strain development of Gluconobacter oxydans: Complementation of non-functional metabolic pathways and increase of carbon flux / Ines Kiefler ; Gutachter: Michael Bott, Ulrich Schulte ; Betreuer: Michael Bott." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2016. http://d-nb.info/1113747854/34.
Full textPham-Schönwetter, Oliver [Verfasser], Harald [Akademischer Betreuer] Gröger, and Harald [Gutachter] Gröger. "Enzymatischer Abbau von Nitroaromaten mit einer En-Reduktase aus Gluconobacter oxidans und Nitroreduktasen NfsA und NfsB aus Escherichia coli im Vergleich / Oliver Pham-Schönwetter ; Gutachter: Harald Gröger ; Betreuer: Harald Gröger." Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2019. http://d-nb.info/1201161541/34.
Full textPeters, Björn [Verfasser], Wolfgang [Akademischer Betreuer] Liebl, Armin [Akademischer Betreuer] Ehrenreich, Dirk [Akademischer Betreuer] Weuster-Botz, and Rudi F. [Akademischer Betreuer] Vogel. "Entwicklung eines Systems zur Expression von membranständigen Dehydrogenasen aus einem Metagenom von Essigsäurebakterien in Gluconobacter oxydans / Björn Peters. Gutachter: Wolfgang Liebl ; Dirk Weuster-Botz ; Rudi F. Vogel. Betreuer: Wolfgang Liebl ; Armin Ehrenreich." München : Universitätsbibliothek der TU München, 2013. http://d-nb.info/1046404822/34.
Full textClaret, Carole. "Métabolismes oxydatif et fermentaire du glycérol chez les bactéries : étude physiologique et cinétique de sa conversion en dihydroxyacétone et en 1,3-propanediol." Toulouse, INSA, 1992. http://www.theses.fr/1992ISAT0034.
Full textBarbe, Jean-Christophe. "La combinaison du dioxyde de soufre dans les moûts et vins issus de raisins botrytisés : rôle des bactéries acétiques." Bordeaux 2, 2000. http://www.theses.fr/2000BOR20745.
Full textHoffmeister, Marc. "Untersuchungen zur Physiologie des Essigsäurebakteriums Gluconobacter oxydans 621H." Doctoral thesis, 2006. http://hdl.handle.net/11858/00-1735-0000-0006-AC22-8.
Full textKrajewski, Vera [Verfasser]. "Modifikation des Glucosestoffwechsels in Gluconobacter oxydans / vorgelegt von Vera Krajewski." 2008. http://d-nb.info/992448352/34.
Full textVoss, Jörn. "Auswirkungen der Deletion membranständiger Dehydrogenasen auf Gluconobacter oxydans DSM 7145." Doctoral thesis, 2009. http://hdl.handle.net/11858/00-1735-0000-0006-AD7E-1.
Full textPai, Shun-Chung, and 白舜仲. "Purification and Properties of Ampicillin-synthesizing Enzyme from Gluconobacter oxydans." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/22658835225011330157.
Full textChao, Der Jiang, and 趙德江. "Cloning, expression, and complete nucleotid sequence of gluconobacter oxydans recA gene." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/81539653835534122649.
Full textHoffmeister, Marc [Verfasser]. "Untersuchungen zur Physiologie des Essigsäurebakteriums Gluconobacter oxydans 621H / vorgelegt von Marc Hoffmeister." 2006. http://d-nb.info/981834426/34.
Full textPrust, Christina. "Entschlüsselung des Genoms von Gluconobacter oxydans 621H - einem Bakterium von industriellem Interesse." Doctoral thesis, 2004. http://hdl.handle.net/11858/00-1735-0000-0006-AE2C-2.
Full textHsiao, Hui-chen, and 蕭惠真. "Improvement of Gluconobacter oxydans by using technique of conjugal mutation and gene recombination." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/02201132707801301239.
Full text東海大學
食品科學系
91
The objective of this study is to genetically modify a gluconate producing strain of Gluconobacter oxydans (Henneberg) De Ley (ATCC23771). First, the strain was mutated by conjugative transposon Tn5, and then screening higher yield mutants was performed. Some mutants with higher gluconate production were selected. Mutants, m3, m6 and m9 are approximately 30% higher than the parent strain. The wild type strain of G. oxydans is not saccharolytic. Among the industrial starch hydrolytic enzymes, amylopullulanase (apu) is a powerful one. The apu gene of plasmid TOEAN 1C was contructed in the plasmid pLKY203. The apu gene of this plasmid is successfully expressed by E. coli JM105. Howere, the apu gene is limitedly expressed by Gluconobacter oxydans ATCC23771.
El-Sayed, Mohamed Mostafa Hesham. "Lactose-utilization and gluconic acid production by genetically modified strains of Gluconobacter oxydans /." 2000. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=009195409&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Full textZHUANG, YU-LI, and 莊育梩. "Studies on the production of L-sorbose by immobilized cells of gluconobacter oxydans." Thesis, 1987. http://ndltd.ncl.edu.tw/handle/13179103670911918711.
Full textTsai, Shuo-Wen, and 蔡碩文. "Characterization and kinetics of the ampicillin-synthesizing enzyme from Gluconobacter oxydans CCRC 10383." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/60798184519134054204.
Full text國立臺灣大學
農業化學系
86
The ampicillin-synthesizing enzyme from Gluconobacter oxydans CCRC 10383 was used to synthesize ampicillin from 6-aminopenicillanic acid (6-APA) and phenylglycine methyl ester (PGM). In this study, spectrophotometric method was employed to determine the enzyme activity by detecting the formation of ampicillin. The optimum condition for the synthesis of ampicillin is pH 6.0 and 37 ℃. Under this optimum condition, 78 % of the added 6-APA was converted into ampicillin. The kinetic constants of this enzyme hav
Merfort, Marcel [Verfasser]. "Untersuchungen zur 5-Keto-D-Gluconat-Bildung mit Gluconobacter oxydans / vorgelegt von Marcel Merfort." 2006. http://d-nb.info/981097871/34.
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