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Journal articles on the topic 'Glutathion peroxydase'

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Published: 4 June 2021
Last updated: 22 June 2024

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1

Ouled-Haddou, Hakim, Kahia Messaoudi, Roggiero Lopes Dos Santos, Candice Carola, Yohann Demont, Alexis Caulier, Pascal Vong, et al. "A New Role of Glutathion Peroxydase 4 in Human Erythroblast Enucleation." Blood 134, Supplement_1 (November 13, 2019): 938. http://dx.doi.org/10.1182/blood-2019-122161.

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The Gluthation peroxidase (GPX) enzymes are part of the protective system against lipid peroxydation that includes prevention of oxydation and reduction of already oxidized lipid through enzymatic reactions catalyzed by GSH. GPX4 is one of the five GPX able to incorporate selenium. It is also the only GPX able to directly reduce in the membrane the oxidized fatty acids and cholesterol. Recent reports identified GPX4 as the central inhibitor of ferroptosis, a process during which iron-induced peroxydation of membrane lipids causes a specific cell death that can be reverted by lipophilic antioxydants or by iron chelators. GPX4 has recently been involved during mice erythropoiesis: GPX4-/-mice present a hemolytic anemia and a high apoptotic rate in spleen erythroid progenitors. Although transcriptomics and proteomics found it expressed in human erythroid precursors, its role during human erythropoiesis has not been described. Using an in-vitroerythroid differentiation protocol from CD34+cells obtained from apheresis, we confirmed that GPX4 expression was induced at RNA and protein level during differentiation. RSL3, a specific GPX4 inhibitor, didn't affect early steps of erythropoiesis (i.eclonogenic potential and progenitor amplification) nor the early maturation of erythroid precursors (assessed by sequential CD49d/CD235/CD71 staining) but led to a significant decrease in the enucleation rate as assessed by Hoechst staining using flow cytometry (74%±9 DMSO versus 35%±6 RSL3, p<0.01) and by cytology after MGG staining (58%±10 DMSO versus 27% RSL3±5, p<0.05). This effect was not related to ferroptosis since (i) FIN56 and erastin, two other ferroptosis activators, didn't impact enucleation and (ii) the enucleation defect was not reverted by tocopherol or ferrostatin. Of note, tocopherol reverted lipid peroxydation at cell surface, as shown by Bodipy-C11 staining, showing that enucleation defect and lipid peroxydation were not directly related. These data argue for a specific GPX4 role in the enucleation process, independently to its well-described function in ferroptosis control. Using Western Blot, we observed that RSL3 exposure induced a strong GPX4 depletion in erythroid progenitors while it didn't affect GPX1, another member of the GPX family expressed in erythroid cells. In order to confirm that enucleation defect was related to GPX4 knockdown, we used an Sh-RNA strategy that allowed a 62%±6 GPX4 decrease at RNA level and a 46%±5 at protein level. We observed a significant defect in terminal enucleation in the cells transduced with shGPX4-lentiviruses in comparison with sh-Scramble (59%±5 Sh-Scr versus 39%±6 Sh-Gpx4, p<0.05). Isopentylpyrophosphate, an intermediate component of cholesterol synthesis that also acts as a substract of selenoprotein synthesis, restored partially both GPX4 expression and erythroblast enucleation. We investigated then whether GPX4-knock-down affected quantitatively or qualitatively the membrane lipid content, which was shown to be involved in the enucleation process. Addition of Cholesterol to RSL3 in the medium partially restored the enucleation rate. However, lipidomics failed to show any significant difference in the total membrane lipid content (and particularly in the cholesterol content) after RSL3 exposure in comparison to DMSO. Since cholesterol is particularly abundant in the lipid rafts, we investigated whether the lipid distribution was qualitatively altered within the cell membrane. We observed a disruption of membrane lipid rafts when cells were exposed to RSL3, as shown by a 60%±12 decrease in the mean cholera toxin fluorescence intensity. GPX4 presence in lipid rafts was confirmed using immunofluorescence showing their co-localization at cell surface in human primary erythroblasts. Since lipid rafts play a role in the contractile ring that separates pyrenocyte from reticulocyte, we evaluated the myosin-light chain phosphorylation using flow cytometry and Western Blot and found it drastically decreased in GPX4-knockdown conditions. In summary, we identified GPX4 as a new actor of human terminal erythroid differentiation, independently to its function in ferroptosis control. We described its interaction at cell surface with lipid rafts that are required for the assembly of the contractile ring and cytokinesis leading to the nucleus extrusion. Disclosures No relevant conflicts of interest to declare.
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2

Mami-Soualem, Z., N. Brixi, C. Beghdad, and M. Belarbi. "Effet antioxydant et antihyperglycémiant du seigle (Secale cereale L.) et du sorgho (Sorghum bicolor L.) chez le rat Wistar rendu diabétique." Phytothérapie 16, S1 (September 5, 2018): S273—S283. http://dx.doi.org/10.3166/phyto-2018-0013.

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Le changement des habitudes alimentaires au cours de ces dernières années a abouti à l’apparition des maladies dites de civilisation, parmi elles le diabète sucré. Des approches diétothérapeutiques privilégient la consommation des produits céréaliers sous la forme la plus complète possible. Ce travail a pour but de tester l’efficacité des régimes expérimentaux préparés à base des grains complets des deux céréales, le sorgho (Sorghum bicolor L.) et le seigle (Secale cereale L.), sur la correction de l’hyperglycémie et des marqueurs du stress oxydant associés au diabète chez le rat mâle Wistar. Les résultats montrent que les rats diabétiques, soumis au régime seigle pendant quatre semaines, présentent une diminution significative de la glycémie qui atteint les 53,95 % à la fin de l’expérimentation, ce qui fait que leur taux en glycémie se rapproche de celui des normoglycémiques. Le sorgho blanc présente, d’une part, une diminution significative de la peroxydation lipidique intracellulaire et, d’autre part, une augmentation de l’activité de la glutathion peroxydase, la glutathion réductase ainsi que la vitamine C. Ces approches méthodologiques peuvent, en améliorant la connaissance de l’importance des céréales (fibres alimentaires solubles et des polyphénols) dans l’évolution du diabète, aboutir à des recommandations et à une éducation nutritionnelle du diabétique.
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3

Accominotti, M., P. Dutey, C. Lahet, and JJ Vallon. "Évolution des taux de sélénium et de glutathion peroxydase sanguins de sportifs de haut niveau." Science & Sports 6, no. 3 (September 1991): 165–72. http://dx.doi.org/10.1016/s0765-1597(05)80249-7.

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4

Anggraeni, Ratna, Arif Dermawan, and F. Febri Wisudawan. "The Association of Glutathion Peroxydase-1 Serum and Sensorineural Hearing Lossin MDR TB Patients with Kanamycin Therapy." Indian Journal of Public Health Research & Development 11, no. 2 (February 1, 2020): 2032. http://dx.doi.org/10.37506/v11/i2/2020/ijphrd/195130.

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5

Anggraeni, Ratna, Arif Darmawan, and F. Febri Wisudawan. "The Association of Glutathion Peroxydase‑1 Serum and Sensorineural Hearing Lossin MDR TB Patients with Kanamycin Therapy." Indian Journal of Public Health Research & Development 11, no. 1 (January 1, 2020): 1102. http://dx.doi.org/10.37506/v11/i1/2020/ijphrd/193986.

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6

Remigi, M., S. Barbier, and P. Kaminsky. "Corrélation entre le stade clinique et les taux sériques de glutathion peroxydase et de zinc dans la démence d'Alzheimer." La Revue de Médecine Interne 17 (January 1996): 486s. http://dx.doi.org/10.1016/s0248-8663(97)81119-8.

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7

Adli, D. E. H., K. Kahloula, M. Slimani, M. Brahmi, and M. Benreguieg. "Effets prophylactiques de l’huile essentielle de Syzygium aromaticum chez les rats wistar en développement coexposés au plomb et au manganèse." Phytothérapie 16, S1 (December 2018): S1—S7. http://dx.doi.org/10.3166/phyto-2019-0149.

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L’objectif de cette étude est d’évaluer les modifications du statut oxydatif au niveau cérébral, induites par la coexposition au plomb (0,2 %) et au manganèse (4,79 g/l) chez des jeunes rats wistar durant la période de gestation et de lactation, et de tester l’efficacité de l’huile essentielle de clou de girofle (HEC), Syzygium aromaticum, pour rétablir ou non les effets néfastes de ces deux métaux, et cela par une injection intrapéritonéale de 0,1 ml d’HEC/kg et par jour durant une période de 21 jours. La caractérisation de cette huile essentielle par chromatographie en phase gazeuse couplée à la spectrométrie de masse indique que les composants majeurs sont : eugénol (80,83 %), acétate d’eugényle (10,48 %) et β-caryophyllène (7,21 %). La coexposition chronique a permis d’observer une perturbation dans les activités enzymatiques antioxydantes (superoxyde-dismutase, glutathion-peroxydase et catalase) chez les rats intoxiqués comparés aux rats témoins. En effet, l’étude histologique au niveau du cortex cérébral et du cervelet a montré des lésions très marquées traduites par une dégénérescence des cellules nerveuses et l’activation des microglies. Par ailleurs, l’administration d’HEC a rétabli l’activité des différentes enzymes antioxydantes avec une nette amélioration de l’architecture tissulaire cérébrale chez les rats intoxiqués et traités par HEC.
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8

P. O., Atte, Zahraddeen D., Adulrashid M., and Daudu O. M. "Effect of Taurine and Sex on oxidative status of West African dwarf Sheep." Nigerian Journal of Animal Production 49, no. 6 (September 11, 2023): 19–23. http://dx.doi.org/10.51791/njap.v49i6.3840.

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Several studies have been carried out on the oxidative status of West African Dwarf (WAD) sheep as influenced by series of antioxidants. However, not much is documented on the use of taurine as an antioxidant. Thirty two WAD sheep involving both sexes (16 ewes and 16 rams) with an average weight of 12kg were fed taurine supplemented diets and evaluated in a Completely Randomized Design involving four dietary treatments with four replicates of two animals each containing 0, 0.5, 1 and 1.5% levels of iinclusion of taurine. The study was carried out during the wet (July-October) and dry (January-April) seasons. Blood sample was collected and the following oxidative parameters were analyzed; catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST) and glutathione peroxidase (GPX). The result shows CAT activity increases significantly (P<0.05) with 1.5% inclusion level having the highest activity (107.08U/) while the control had the least (100.81U/mL). The least value of SOD (5.14U/mL) was recorded in animals fed the 0% dietary taurine while animals fed 1.5% had the highest activity (9.87U/mL). GST activity increases significantly (P<0.05) from 1.03 U/mL (0%) to 4.68U/mL (1.5%). The GPX varied from 1.14U/mL (0%) to 2.89U/mL (1.5%). CAT (108.61U/mL) is significantly (P<0.05) higher in ram compared to ewe (99.59U/mL). SOD (7.87U/mL) activity was higher in the ewe compared to ram (6.51U/mL). In addition, GST activity was higher in ram (3.06U/mL) compared to ram (1.95U/mL. GPX activity was not influenced by sex. It was concluded from this study that taurine supplemented diet had better antioxidant defense mechanism and significant effect on the sex of investigated WAD sheep. It was recommended that diet of WAD sheep could be supplemented with taurine for higher oxidative stability and protection against tissue damage. Plusieurs études ont été menées sur le statut oxydatif des moutons West African Dwarf (WAD) tel qu'il est influencé par une série d'antioxydants. Cependant, peu de choses sont documentées sur l'utilisation de la taurine comme antioxydant. Trente-deux moutons WAD impliquant les deux sexes (16 brebis et 16 béliers) d'un poids moyen de 12 kg ont été nourris avec des régimes enrichis en taurine et évalués dans un plan complètement randomisé impliquant quatre traitements alimentaires avec quatre répétitions de deux animaux contenant chacun 0, 0,5, 1 et 1,5% niveaux d'inclusion de taurine. L'étude a été réalisée pendant les saisons humides (juillet-octobre) et sèches (janvier-avril). Un échantillon de sang a été prélevé et les paramètres oxydatifs suivants ont été analysés ; catalase (CAT), superoxyde dismutase (SOD), glutathion-S-transférase (GST) et glutathion peroxydase (GPX). Le résultat montre que l'activité CAT augmente de manière significative (P<0,05) avec un niveau d'inclusion de 1,5 % ayant l'activité la plus élevée (107,08 U/) alors que le contrôle en avait le moins (100,81 U/mL). La plus faible valeur de SOD (5,14U/mL) a été enregistrée chez les animaux nourris avec la taurine alimentaire à 0 %, tandis que les animaux nourris avec 1,5 % avaient l'activité la plus élevée (9,87U/mL). L'activité GST augmente significativement (P<0,05) de 1,03 U/mL (0 %) à 4,68 U/mL (1,5 %). Le GPX variait de 1,14U/mL (0 %) à 2,89U/mL (1,5 %). Le CAT (108,61U/mL) est significativement (P<0,05) plus élevé chez le bélier que chez la brebis (99,59U/mL). L'activité de SOD (7,87U/mL) était plus élevée chez la brebis que chez le bélier (6,51U/mL). De plus, l'activité de la GST était plus élevée chez le bélier (3,06 U/mL) que chez le bélier (1,95 U/mL. L'activité de la GPX n'était pas influencée par le sexe. Il a été conclu de cette étude que l'alimentation enrichie en taurine avait un meilleur mécanisme de défense antioxydant et un effet significatif. Il a été recommandé que le régime alimentaire des moutons WAD soit complété par de la taurine pour une meilleure stabilité oxydative et une protection contre les lésions tissulaires.
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9

Oderinwale, O. A., B. O. Oluwatosin, M. O. Onagbesan, E. O. Adekunle, A. Y. Shuaibu, S. D. Amosu, A. J. Adeyemo, O. M. Kuye, J. O. Olalere, and I. T. Ajewole. "Effects of dietary inclusion of turmeric (Curcuma longa L.) powder on oxidative stress and cortisol concentration in goats during pregnancy and onset of postpartum." Nigerian Journal of Animal Production 48, no. 6 (January 18, 2022): 374–90. http://dx.doi.org/10.51791/njap.v48i6.3325.

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Oxidative stress is a chemical stress caused by imbalance between the production of free radicals and the ability of organisms to absorb their excess. It is extremely dangerous because affected animals may not exhibit physical symptom(s). It induces a stress with cellular damage that if not adequately restored by antioxidant makes the organism sensitive to serious degenerative disorders. Against this background, a study on oxidative stress in Kalahari Red (KR), West African Dwarf (WAD) and KalaWAD goats fed graded levels of turmeric (Curcuma longa L.) powder (TP) during pregnancy and after kidding was evaluated. Forty five goats comprising of 15 goats/breed and divided into five goats per/breed/treatment were used. Dietary treatments which included concentrate diet (CD) as TM-0; CD+2g/kg TP as TM-2; and CD+5g/kg TP as TM-5 were fed for 162days. Blood samples were collected from the goats at the beginning of the experiment; 1st and 2nd trimesters; and within 24hrs after kidding for determination of antioxidant enzymes activities like Superoxide dismutase (SOD); Glutathione peroxidase (GSH-Px); Glutathione (GSH); Thiobarbituric reactive substances (TBARS) and Cortisol spectrophotometrically. Results obtained revealed that WAD goats had highest (p<0.05) reduction by 3.57u/mL for TBARS. The KR goats had increment (p<0.05) by 1.56u/mL for GSH-Px and decrease (p<0.05) by 1.78ng/mL for cortisol concentration. SOD was increased (p<0.05) by 0.50u/ml for KalaWAD goats. TP inclusion at 2g/kg improved (p<0.05) values for SOD (1.21u/mL) and GSH-Px (0.85u/mL), while GSH (2.28u/mL) was improved by TP inclusion at 5g/kg. KalaWAD goats fed TM-2 had highest (p<0.05) reduction in TBARS value by 2.70u/mL, whereas KalaWAD goats fed TM-5 had improved (p<0.05) value for SOD (1.91u/mL). KR goats fed TM-2 and TM-5 had improvement and reduction in the values of GSH-Px by 5.41u/mL and cortisol concentration by 2.93ng/mL respectively. It is concluded that breeds of goat, turmeric powder inclusion and their interaction influenced oxidative stress and cortisol concentration. Le stress oxydatif est un stress chimique causé par un déséquilibre entre la production de radicaux libres et la capacité des organismes à absorber leur excès. C'est extrêmement dangereux car les animaux affectés peuvent ne pas présenter de symptôme(s) physique(s). Il induit un stress avec des dommages cellulaires qui, s'il n'est pas correctement restauré par des antioxydants, rend l'organisme sensible à de graves troubles dégénératifs. Dans ce contexte, une étude sur le stress oxydatif chez les chèvres Kalahari Red (KR), West African Dwarf (WAD) et Kala WAD nourries à des niveaux gradués de poudre de curcuma (Curcuma longa L.) (TP) pendant la grossesse et après la mise bas a été évaluée. Quarante-cinq chèvres comprenant 15 chèvres/race et divisées en cinq chèvres par/race/traitement ont été utilisées. Traitements diététiques qui comprenaient un régime concentré (RC) en tant que TM-0 ; CD+2g/kg TP comme TM-2 ; et CD+5g/kg de TP sous forme de TM-5 ont été nourris pendant 162 jours. Des échantillons de sang ont été prélevés sur les chèvres au début de l'expérience; 1er et 2e trimestres ; et dans les 24 heures après la blague pour la détermination des activités des enzymes antioxydantes comme le superoxyde dismutase (SOD) ; Glutathion peroxydase (GSH-Px); glutathion (GSH); Substances réactives thiobarbituriques (TBARS) et cortisol par spectrophotométrie. Les résultats obtenus ont révélé que les chèvres WAD présentaient la réduction la plus élevée (p<0,05) de 3,57u/mL pour les TBARS. Les chèvres KR ont augmenté (p<0,05) de 1,56u/mL pour le GSH-Px et diminué (p<0,05) de 1,78ng/mL pour la concentration de cortisol. La SOD a été augmentée (p<0,05) de 0,50 u/ml pour les chèvres KalaWAD. L'inclusion de TP à 2g/kg a amélioré (p<0,05) les valeurs de SOD (1,21u/mL) et de GSH-Px (0,85u/mL), tandis que le GSH (2,28u/mL) a été amélioré par l'inclusion de TP à 5g/kg. Les chèvres KalaWAD nourries au TM-2 présentaient la réduction la plus élevée (p<0,05) de la valeur TBARS de 2,70u/mL, tandis que les chèvres KalaWAD nourries au TM-5 avaient une valeur améliorée (p<0,05) pour la SOD (1,91u/mL). Les chèvres KR nourries avec TM-2 et TM-5 ont eu une amélioration et une réduction des valeurs de GSH-Px de 5,41u/mL et de la concentration de cortisol de 2,93ng/mL respectivement. Il est conclu que les races de chèvres, l'inclusion de poudre de curcuma et leur interaction ont influencé le stress oxydatif et la concentration de cortisol.
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Bogdanova, I. A., and A. M. Gerasimov. "Influence of mechanical injury on glutathione redox-system state in bone marrow of rats." N.N. Priorov Journal of Traumatology and Orthopedics 2, no. 1-2 (December 28, 1995): 44–46. http://dx.doi.org/10.17816/vto99593.

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Dynamics of activity of glutathione: dehydroascorbate oxyreductase, glutathione peroxydase, glutathione reductase was studied in bone marrow of rats with closed bone fractures during different terms after injury infliction. On day 30 of traumatic disease the activity of glutathione: dehydroascorbate oxyreductuse, calculated jn 1 mg DNA, decreased on 27% that increased the sensitiveness of bone marrow cells to the deficit of ascorbinic acid. The detected stability of glutathione reductase showed the possibility of glutathione application in pharmcotherapy of traumatic disease.
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11

Ademu, L. A., G. James, J. R. Rimamfenten, and E. E. Oko. "Trimethyl glycine (TMG) influences on blood biochemistry and organ indices of heat stressed growing rabbits." Nigerian Journal of Animal Production 49, no. 5 (May 26, 2023): 127–37. http://dx.doi.org/10.51791/njap.v49i5.3771.

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Trimethyl glycine has been supplemented in livestock diets for decades due its role as an essential osmo-protectant, protecting proteins, enzymes, inhibiting cellular apoptosis and reducing energy expenditure, under osmotic stress. A study was conducted to determine the effect of trimethyl glycine (TMG) on blood biochemistry and organ indices of heat-stressed growing rabbits. Thirty rabbits were randomly allotted to three experimental treatments. Each treatment was replicated five times with two rabbits per replicate in a completely randomized design. A one-week adjustment period was given before commencement of the feeding trial. Trimethyl glycine was supplemented at 0, 1.0 and 1.5%. Results indicated that temperature humidity index (THI) in the afternoon was higher than THI in the morning by 27.16%, indicating the absence of heat stress in the morning and the presence of very severe heat stress in the afternoon. There were significant (P<0.05) differences observed in malondialdehyde (MDA) and catalase (CAT) levels. No significant differences (P>0.0) across treatments were observed for superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity. There were significant (P<0.05) differences observed in aspartate transaminase and alanine transaminase. Other parameters such as globulin, albumin, total protein, low-density lipoprotein, high-density lipoprotein, very low-density lipoprotein, total cholesterol, triglycerides and alkaline phosphatase were not significant (P>0.05). There were significant (P<0.05) differences observed in white blood cell and basophils levels. Kidney and liver weights were also significantly (P<0.05) affected. It can be concluded that TMG had positive effects on blood biochemistry and organ indices of growing heat-stressed rabbits and is recommended up to 1.5% in heat-stressed growing rabbit diet. La triméthyl glycine est supplémentée dans l’alimentation du bétail depuis des décennies en raison de son rôle d’osmo-protecteur essentiel, protégeant les protéines, les enzymes, inhibant l’apoptose cellulaire et réduisant la dépense énergétique, sous stress osmotique. Une étude a été menée pour déterminer l’effet de la triméthyl glycine (TMG) sur la biochimie sanguine et les indices d’organes de lapins en croissance stressés par la chaleur. Trente lapins ont été répartis au hasard dans trois traitements expérimentaux. Chaque traitement a été répété cinq fois avec deux lapins par répétition dans une conception entièrement randomisée. Une période d’ajustement d’une semaine a été accordée avant le début de l’essai d’alimentation. La triméthyl glycine a été complétée à 0, 1,0 et 1,5 %. Les résultats ont indiqué que l’indice d’humidité de la température (IHT) de l’après-midi était supérieur à celui du matin de 27,16 %, indiquant l’absence de stress thermique le matin et la présence d’un stress thermique très sévère l’après-midi. Des différences significatives (P < 0,05) ont été observées dans les niveaux de malondialdéhyde (MDA) et de catalase (CAT). Aucune différence significative (P>0,0) entre les traitements n’a été observée pour l’activité de la superoxyde dismutase (SOD) et de la glutathion peroxydase (GPx). Il y avait des différences significatives (P<0,05) observées dans l’aspartate transaminase et l’alanine transaminase. D’autres paramètres tels que la globuline, l’albumine, les protéines totales, les lipoprotéines de basse densité, les lipoprotéines de haute densité, les lipoprotéines de très basse densité, le cholestérol total, les triglycérides et la phosphatase alcaline n’étaient pas significatifs (P>0,05). Des différences significatives (P < 0,05) ont été observées dans les taux de globules blancs et de basophiles. Le poids des reins et du foie était également significativement (P<0,05) affecté. On peut conclure que le TMG a eu des effets positifs sur la biochimie sanguine et les indices d’organes des lapins en croissance stressés par la chaleur et est recommandé jusqu’à 1,5 % dans l’alimentation des lapins en croissance stressés par la chaleur.
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12

Yarosh, Oleg Aleksandrovich. "The role of neurotransmitter systems separate the hemispheres of the brain in action the new antiepileptic compounds AGB-31." Reviews on Clinical Pharmacology and Drug Therapy 11, no. 2 (June 15, 2013): 31–35. http://dx.doi.org/10.17816/rcf11231-35.

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Compound AGB-31, a monocarbamate derivative, is shown to possess a high antiepileptic activity. The mechanisms of antiepileptic action are connected with significant increase in glutamic acid decarboxylase activity in the left hemisphere of the brain, with trend of the glutamate content decrease in the left hemisphere and the tendency to increase GABA in both hemispheres. AGB-31 significantly (more than 3-fold) increases syntase nitric oxide activity in the left hemisphere and has a tendency to reduce the NO content in both hemispheres. AGB-31 significantly (by 63.4%), reduced glutathione peroxydase activity in the right hemisphere without changing it in the left, with a tendency to increase the activity of glutathione reductase in both hemispheres.
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13

Ayeni, A. O., M. Adegbenro, O. E. Dick, E. S. Oyedokun, I. O. Olasehinde, and J. O. Agbede. "Performance, haemato-biochemical indices and antioxidants status of broiler chickens fed diets supplemented with avocado-bamboo composite leaf mix." Nigerian Journal of Animal Production 49, no. 1 (February 25, 2022): 293–303. http://dx.doi.org/10.51791/njap.v49i1.3428.

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Phytogenic feed additives have been suggested to augment nutrient utilization in the gastrointestinal tract by enhancing production of digestive secretions and enzymatic activity. Such effects on gastrointestinal morphology have been postulated to increase the nutrient digestibility in poultry. However, research on avocado—bamboo composite mix on broiler chickens are limited, this thus forms the focus of this study.A six week feeding trial was conducted to evaluate the growth, haemato-biochemical indices and antioxidants status of broiler chickens fed varying levels of composite leaf mix (CLM). The avocado and bamboo leaves used were manually harvested, air-dried and milled to produce leaf meal. The two leaf meals were then mixed in ratio 1:1 to form the CLM. The CLM was included in broiler basal diets at 0, 4, 8 and 12g/kg levels designated diets I – IV, respectively. Ninety six day-old Cobb broiler chicks were randomly allotted to four treatments of three replicates with eight chicks each. At the end of the feeding trial, the result revealed that average final weight of chicken fed diets II (2533.33±66.67g) and III (2406.55±30.59g) were similar but higher than those fed the control (2216.67±29.83g). The same pattern was observed for total weight gain. The feed conversion ratio (FCR) was not affected although birds fed diet II had the best FCR (2.08±0.06).No significant (P>0.05) difference was observed in all the haematological indices save mean cell volume and mean cell haemoglobin which was highest in birds fed 4g/kg CLM supplemented diet (149.87±24.73fL and 49.95±8.25 pg/cell, respectively) as compared to 111.32±16.56fL and 37.12±5.55pg/cell in those supplemented 12g/kg CLM. The same pattern was repeated in percentage lymph .Among the serum biochemistry parameters measured, only Aspartate transaminase was affected and highest in birds fed diet supplemented with 4g/kg CLM (102.52±5.88µ/L) and lowest in those fed 12g/kg CLM (99.33±5.13µ/L). The superoxide dismutase, catalase and Glutathione peroxidase increased with increased CLM supplementation. The growth, haematological, serum biochemical and antioxidant parameters obtained from this study suggested that the CLM has no adverse effect on the health status of broiler chickens but on the other hand improved the growth, blood serum and serum antioxidant profiles. Des additifs alimentaires phytogéniques ont été suggérés pour augmenter l'utilisation des nutriments dans le tractus gastro-intestinal en améliorant la production de sécrétions digestives et l'activité enzymatique. De tels effets sur la morphologie gastro-intestinale ontété postulés pour augmenter la digestibilité des nutriments chez la volaille. Cependant, les recherches sur le mélange composite avocat-bambou sur les poulets de chair sont limitées, c'est donc l'objet de cette étude. Un essai d'alimentation de six semaines a été mené pour évaluer la croissance, les indices hémato-biochimiques et le statut en antioxydants de poulets à griller nourris à des niveaux variables de mélange de feuilles composites (MFC). Les feuilles d'avocat et de bambou utilisées ont été récoltées manuellement, séchées à l'air et broyées pour produire de la farine de feuilles. Les deux farines de feuilles ont ensuite été mélangées dans un rapport 1:1 pour former le MFC. Le MFC a été inclus dans les régimes de base des poulets de chair à des niveaux de 0, 4, 8 et 12 g/kg désignés régimes I à IV, respectivement. Quatre-vingt-seize poussins de chair Cobb âgés d'un jour ont été répartis au hasard dans quatre traitements de trois répétitions avec huit poussins chacun. À la fin de l'essai d'alimentation, le résultat a révélé que le poids final moyen des poulets nourris avec les régimes II (2533,33 ± 66,67 g) et III (2406,55 ± 30,59 g) était similaire mais supérieur à celui des poulets nourris avec le témoin (2216,67 ± 29,83 g). La même tendance a été observée pour le gain de poids total. Le taux de conversion alimentaire (TCA) n'a pas été affecté bien que les oiseaux nourris avec le régime II aient eu le meilleur TCA (2,08 ± 0,06). Aucune différence significative (P>0,05) n'a été observée dans tous les indices hématologiques, sauf le volume cellulaire moyen et l'hémoglobine cellulaire moyenne qui le plus élevé chez les oiseaux nourris avec 4 g/kg de régime supplémenté en MFC (149,87 ± 24,73 fLet 49,95 ± 8,25 pg/cellule, respectivement) par rapport à 111,32 ± 16,56 fLet 37,12 ± 5,55 pg/cellule chez ceux supplémentés en 12 g/kg de MFC. Le même schéma a été répété en pourcentage de lymphe. Parmi les paramètres biochimiques sériques mesurés, seule l'aspartate transaminase était affectée et la plus élevée chez les oiseaux nourris avec un régime supplémenté de 4 g/kg de MFC (102,52 ± 5,88 µ/L) et la plus faible chez ceux nourris avec 12 g/kg de MFC (99,33 ± 5,13 µ/L). La superoxyde dismutase, la catalase et la glutathion peroxydase ont augmenté avec l'augmentation de la supplémentation en MFC. Les paramètres de croissance, hématologiques, biochimiques sériques et antioxydants obtenus à partir de cette étude suggèrent que le MFC n'a pas d'effet néfaste sur l'état de santé des poulets de chair mais améliore en revanche les profils de croissance, de sérum sanguin et d'antioxydants sériques.
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Dubois, M., M. Croset, A. F. Prigent, G. Némoz, and M. Lagarde. "Cyclic nucleotide phosphodiesterase and glutathione peroxydase activities in heart of rats fed diets containing different oils." Journal of Molecular and Cellular Cardiology 22 (June 1990): S20. http://dx.doi.org/10.1016/0022-2828(90)92003-w.

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15

Mostafa, E. M., and A. M. A. Hassan. "The ameliorative effect of selenium on Azolla caroliniana grown under UV-B stress." Résumés des conférences 95, no. 1 (February 2, 2015): 20–26. http://dx.doi.org/10.7202/1031954ar.

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Exposure ofAzollaplants to UV-B radiation for 6 h resulted in a decrease in biomass and relative growth rate (RGR), which coincided with an increase in doubling time (DT) as compared with the control. Also, the protein content decreased. On the other hand, hydrogen peroxyde (H2O2) and malondialdehyde (MDA) accumulated significantly in UV-treatedAzollaplants. Conversely, the addition of selenium (Se) at 1 ppm resulted in a significant increase in biomass and protein content of untreated and UV-treatedAzollaplants, and a significant reduction in both H2O2and MDA. Moreover, the addition of Se to UV-treated and untreatedAzollaplants resulted in a significant increase in total ascorbate and total glutathione (GSH) contents compared with the control and UV-stressedAzollaplants. Also, glutathione redox potential (GSH/TG) increased significantly in UV-treatedAzollaplants in the presence of Se. There also was a significant increase (38%) in ascorbate peroxidase (APX) activity in UV-treated plants compared with the control. APX activity in the presence of Se did not change significantly compared with the control. Glutathione reductase (GR) activity increased significantly in UV-treatedAzolla, while glutathione peroxidase (GSH-PX) activity did not. On the other hand, both GSH-PX and GR activity in untreated and UV-treatedAzollaplants were significantly enhanced by the application of Se to the nutrient media at a concentration of 1 ppm. Therefore, we can conclude that Se protectsAzollaplants from UV-B stress.
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Marconnet, P., F. Tessier, H. Hida, and A. Favier. "Human Muscular Glutathione Peroxydase Activity Changes with Acute and Chronic Exercise in Young Healthy Subjects Supplemented with Selenium." Clinical Science 87, s1 (January 1, 1994): 79–80. http://dx.doi.org/10.1042/cs087s079a.

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17

Herbette, Stéphane, Denis Tourvieille de Labrouhe, Joël R. Drevet, and Patricia Roeckel-Drevet. "Transgenic tomatoes showing higher glutathione peroxydase antioxidant activity are more resistant to an abiotic stress but more susceptible to biotic stresses." Plant Science 180, no. 3 (March 2011): 548–53. http://dx.doi.org/10.1016/j.plantsci.2010.12.002.

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18

Nganou-Gnindjio, C. N., J. F. Tsasse Fokou, J. R. Nkeck, C. A. Pieme, and M. S. Doualla. "Investigation of malondialdehyde, catalase, glutathione peroxydase and FRAP as biomarker for oxidative stress linked to hyperuricemia in a sub-Saharan population with heart failure." Archives of Cardiovascular Diseases Supplements 15, no. 1 (January 2023): 122. http://dx.doi.org/10.1016/j.acvdsp.2022.10.236.

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19

Serviddio, Gaetano, Nicola Di Venosa, Antonio Federici, Donato D'Agostino, Tiziana Rollo, Filomena Prigigallo, Emanuele Altomare, Tommaso Fiore, and Gianluigi Vendemiale. "Brief hypoxia before normoxic reperfusion (postconditioning) protects the heart against ischemia‐reperfusion injury by preventing mitochondria peroxyde production and glutathione depletion." FASEB Journal 19, no. 3 (March 2005): 354–61. http://dx.doi.org/10.1096/fj.04-2338com.

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20

AUROUSSEAU, B. "Les radicaux libres dans l’organisme des animaux d’élevage : conséquences sur la reproduction, la physiologie et la qualité de leurs produits." INRAE Productions Animales 15, no. 1 (February 12, 2002): 67–82. http://dx.doi.org/10.20870/productions-animales.2002.15.1.3688.

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Les phénomènes radicalaires sont omniprésents dans la vie des animaux domestiques. Les radicaux libres oxygénés en sont à l’origine : leur structure électronique déséquilibrée leur confère une grande réactivité sur les constituants tissulaires qui leur permet d’entrer en jeu dans le contrôle d’une grande variété de réactions d’oxydo-réduction indispensables à la vie et à la croissance. Ils sont formés de façon continue dans l’organisme sain, en particulier par fuite d’électrons à partir de la chaîne respiratoire et au cours de l’élimination des composés tissulaires peroxydés. Les stress de toutes natures augmentent leur flux, épuisent les réserves de l’organisme en composés protecteurs qui les capturent (en particulier les vitamines et le glutathion) et augmentent donc les besoins correspondants des animaux. Pour finir, ils peuvent conduire à l’altération des structures cellulaires. Les conséquences intéressent une grande variété de fonctions animales, de façon directe (immédiatement après une série de chocs) ou indirecte (affaiblissement à long terme de l’organisme). Les attaques radicalaires peuvent ainsi conduire à une diminution du pouvoir fécondant des spermatozoïdes, à une baisse de la fertilité des femelles, à des mortalités embryonnaires et à une baisse de la vitalité des nouveau-nés. Par ailleurs, elles peuvent ralentir la croissance des animaux et conduire à l’obtention de carcasses et de viandes de qualités inférieures au potentiel des animaux. Ces baisses de qualité sont caractérisées par une durée de conservation plus courte et par une diminution de la teneur des tissus en composés intéressants pour la santé de l’homme. Le plus grand soin doit donc être apporté à la conduite des animaux d’élevage, afin de respecter l’équilibre entre l’attaque et la défense de l’organisme des animaux domestiques. Documents à télécharger
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21

Vignon, Christine, Sebastien Lachot, Yves Levern, Beatrice Herault, Philippe Rosset, Franck Perrotin, Emmanuel Gyan, Jorge Domenech, and Olivier Herault. "Reactive Oxygen Species Level and GPX3 Expression in Human CD34+CD38− Progenitors Harvested From Cord Blood, Adult Bone Marrow and Peripheral Blood." Blood 120, no. 21 (November 16, 2012): 4743. http://dx.doi.org/10.1182/blood.v120.21.4743.4743.

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Abstract Abstract 4743 Redox signaling has emerged as an important regulator of hematopoietic stem cell (HSC) self-renewal and lifespan. It is established that murine HSCs have a low level of reactive oxygen species (ROS) which is correlated with stem cells properties (Ito K et al, Nat. Medicine, 2006; Jang YY and Sharkis SJ, Blood, 2007). Moreover, it was recently reported that Gpx3, a gene encoding for the ROS scavenger glutathione peroxydase 3, is a determinant of the self-renewal of HSCs (Herault O et al, J. Exp. Med., 2012). All these studies were performed in murine hematopoiesis and the objective of the present study was to quantify the ROS level and the GPX3 expression in human CD34+CD38- progenitor cells (vs CD34+CD38+ cells) harvested from bone marrow, cord blood and peripheral blood. Human bone marrow (BM) samples were obtained from patients (n=6) undergoing orthopedic surgery (Department of Orthopedic Surgery, University Hospital, Tours, France), ombilical cord bloods were obtained from women (n=5) after vaginal deliveries (Department of Gynecology and Obstetrics, University Hospital, Tours, France), and G-CSF mobilized peripheral blood stem cells were obtained by leukapheresis from patients (n=5) of the Department of Clinical Hematology (University Hospital, Tours, France). All samples were collected from patients informed and consenting following a procedure approved by the ethical committee. The intracellular H2O2 level was quantified by flow cytometry. The cells were incubated with 10 μM DCF-DA, 5 μL of APC-Cy7-conjugated anti-CD45 mAb (A20), 2.5 μL of PE-Cy7-conjugated anti-CD34 mAb (8G12) and 2.5 μL of APC-conjugated anti-CD38 mAb (HB7) at 37°C for 10 min and then analyzed. Neutrophils, lymphocytes, and monocytes were identified according to CD45/SSC gating. The subpopulation SSClowCD45intCD34+ has been split into two fractions according to the expression of CD38. The expression of GPX3 was measured by quantitative RT-PCR (vs. GAPDH) in CD34+CD38- and CD34+CD38+ FACS (MoFloTM, Beckman Coulter)-sorted cells using the same gating strategy as previously mentioned for the ROS measurement. We observed that among the different cell subpopulations, the CD34+CD38- fraction was the one which expressed the lowest level of ROS, which was higher in the CD34+CD38+ fraction in all analyses. This difference in marrow, cord blood and peripheral blood samples was on average (+/−ecm) 3.7+/−0.6, 4.0+/−2.3 and 1.3+/−0.1, respectively. Regarding the GPX3 expression in CD34+ cells, we found a high level in the marrow samples, a moderate level in the cord blood samples and a low level in the peripheral blood samples. The GPX3 expression in CD34+CD38- fraction from bone marrow, cord blood and peripheral blood was on average (+/−ecm) 4.6+/−1.2, 3.2+/−0.4 and 1.3+/−0.1 higher than in CD34+CD38+ cells, respectively. The ROS level and GPX3 expression observed in human CD34+CD38- progenitors from bone marrow and cord blood are in line with those found in mouse hematopoiesis. It's interresting to note that the mobilization process probably modify these parameters in peripheral blood progenitors. In summary, all these data suggest a key role of GPX3 in the human hematopoiesis and that ROS level could provide a good approach to functionally isolate primitive human HSCs from bone marrow and cord blood, but not from peripheral blood after G-CSF mobilization. Disclosures: No relevant conflicts of interest to declare.
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22

Leclerc, Joan, Debeissat Christelle, Socco-Lucca Marion, Ducrocq Elfi, Gouilleux Fabrice, Stasia Marie José, and Olivier Herault. "Influence of NADPH Oxidase Activity On the Reactive Oxygen Species Level in Human Leukemic Cells." Blood 120, no. 21 (November 16, 2012): 4801. http://dx.doi.org/10.1182/blood.v120.21.4801.4801.

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Abstract Abstract 4801 Redox metabolism plays an important role in self-renewal and differentiation of hematopoietic and leukemic cells. Reactive oxygen species (ROS) level is highly regulated. This regulation involves antioxydative enzymes and it has been recently described that leukemic stem cells (LSC) overexpress glutathione peroxydase 3 (Herault O et al, J. Exp. Med, 2012). This overexpression is associated with a decrease in ROS level and p38MAPK inactivation. ROS level in leukemic cells could be also regulated by the activity of ROS producers, such as NADPH oxidase, known to catalyze an electron transfer from NADPH to oxygen producing superoxides which could generate other downstream ROS. The expression of this enzymatic complex (NOX family, 6 isoforms) has been established in the plasma cell membrane of normal CD34+ hematopoietic progenitors (Piccoli C et al, Biochem. Biophys. Res. Commun., 2007). The aim of this study was to decipher the expression of NADPH oxydase components in various human acute myeloid leukemia (AML) Different leukemic cell lines were used according FAB classification: KG1a (MO/M1), KG1 (M1), HL60 (M2), Kasumi 1 (M2), NB4 (M3), ML2 (M4), THP1 (M5), U937 (M5), MV4–11 (M5), K562 (M6). The cells were cultured (2.105 cells/mL, 37°C in 95% humidified air and 5% CO2) in RPMI 1640 with 20mmoL/L L-glutamine supplemented with 10% FCS, 100 units/mL penicillin G, and 100mg/mL streptomycin. The expression of NOX1, NOX2, NOX3, NOX4, NOX5, DUOX1, DUOX2, P22phox and P40phox, P47phox, P67phox, NOXO1, NOXA1 was quantified by RT-qPCR (Universal Probe Library, Roche). NOX2 and its regulatory subunits expression was quantified by SDS-PAGE and western-blot experiments. The effects of diphenylene iodonium (DPI), a specific NOX inhibitor, were evaluated by ROS quantification using dichlorodihydrofluorescein diacetate (DCF-DA) staining followed by fluorimetry and flow cytometry analyses. The cells were washed twice in the physiological saline buffer (PBS) without calcium and magnesium, then incubated in PBS complemented with 0.5M MgCl2, 0.9M CaCl2, 20mM glucose (Picciocchi A et al, J. Biol. Chem., 2011) with or without 20μM DPI for 1 hour. The cells were distributed at 106cells per 200μL well in 96 wells plates. DCF-DA (10μM) was added to quantify the ROS level (flow cytometry) and to monitor ROS production kinetic (fluorimetry). NOX family genes expression showed that phagocyte oxidase NOX2 is expressed in all leukemic cell lines. Conversely the NOX2 isoforms were not expressed, or very weakly expressed in leukemic cell lines (NOX3 in KG1a; NOX4 in K562; DUOX1 in KG1a, KG1; DUOX2 in KG1a, KG1, HL60). P22phox, the second cytochrome b558 component was also expressed in all cell lines, this expression being higher than NOX2. The cytochrome b558 components were more expressed in differentiated leukemic cells (granulocytic and monocytic) than in undifferentiated cells (KG1a, KG1). NOX2 regulatory subunits were expressed in all leukemic cell lines, the lower level (especially P40phox, P47phox) being observed in KG1a. Proteins quantification confirmed RNA results. Cytochrome b558 components and regulatory subunits were expressed in all cell lines with a higher level in differentiated leukemias. Interestingly, the regulatory subunits were not observed in KG1a cells. Functional flow cytometry and fluorimetry studies revealed a decrease in ROS production in DPI exposed leukemic cell lines. This effect was higher in monocytic cell lines than in granulocytic and undifferentiated leukemias. In conclusion, NADPH oxidases are present in the AML cell membrane, and NOX contribution to the ROS level is higher in differentiated cells than in immature leukemias. Altogether these results suggest that NADPH oxidase is constitutively active in leukemic cells and influences the ROS level, suggesting a role in the pathophysiology of AML. Disclosures: No relevant conflicts of interest to declare.
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Vignon, Christine, Marie-Thérèse Georget, Yves Levern, Elfi Ducrocq, Marie-Christine Bernard, Marie-Hélène Estienne, Dominique Kerboeuf, et al. "Bone Marrow Mesenchymal Stromal Cells Regulate the Metabolism of H2O2 In Human Leukemic Cells." Blood 116, no. 21 (November 19, 2010): 1058. http://dx.doi.org/10.1182/blood.v116.21.1058.1058.

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Abstract Abstract 1058 Redox metabolism plays an important role in self-renewal and differentiation of hematopoietic cells and it has been recently established by the Guy Sauvageau's group (Institute for Research in Immunology and Cancer, Montréal, QC) that glutathione peroxydase 3 (GPx-3) promotes competitiveness of Hoxa9-Meis1 induced leukemic stem cells in which Gpx3 overexpression is concomitant of a decrease in H2O2 level and inactivation of p38 MAPK (Herault O et al, ASH annual meeting, 2010 - submitted). Leukemic cells located in the bone marrow (BM) are interacting with a microenvironment which plays a crucial role in the development and progression of leukemia. Mesenchymal stromal cells (MSCs) constitute a population of multipotential cells giving rise to the different hematopoietic microenvironmental cells (adipocytes, osteoblasts, chondrocytes, and vascular-smooth muscle-like hematopoietic supportive stromal cells). The aim of our study was to evaluate the effects of MSC-contact on the GPx-3/H2O2/p38 MAPK axis in human leukemic cells and to assess the cell cycle changes associated with the modification of H2O2 metabolism. BM MSCs were obtained from informed and consenting patients undergoing orthopedic surgery, following a procedure approved by the local ethical committee. Nucleated cells harvested from the iliac crest were seeded (5.104 cells per cm2) in α-MEM supplemented with 10% fetal calf serum (FCS), 20 mmol/l L-glutamine, and 100 units/mL penicillin G. Cells were incubated at 37°C in 95% humidified air and 5% CO2. When fully confluent, the layer of adherent cells was trypsinized (0.25% trypsin-EDTA), and the cells were resuspended in culture medium and seeded at 103 cells per cm2 (passage 1 - P1). BM MSCs were used at P2 in all experiments. The three-lineage mesenchymal differentiation of the BM MSCs used was systematically checked by culturing cells in adipogenic, chondrogenic, and osteogenic induction media as previously described (Delorme et al, Blood 2008, 111:2631). The human KG1a leukemic cell line (FAB M0/M1 CD34+ leukemic cells) was cultured in RPMI 1640 with 20 mmoL/L L-glutamine supplemented with 10% FCS, 100 units/mL penicillin G, and 100 mg/mL streptomycin. KG1a cells were seeded at 1.5 105 cells/cm2 and cocultured with MSCs for 72 h. We have defined two distinct localizations of leukemic cells relative to MSC layer: those in supernatant (non-adherent cells) and cells adhering on the surface of MSCs. The expression of antioxydative enzymes, H2O2 level, p38 MAPK activation (T180/Y182), cell cycle, proliferation and immunophenotype of these two cell fractions were evaluated at day 0 and day 3. The expression of SOD1, SOD2, SOD3, CAT, TXN, TXN2, GLRX, GLRX2, GLRX3, GLRX5, PRDX, PRDX2, PDRX3, PRDX4, PRDX5, PRDX6, GPX1, GPX2, GPX3, GPX4, GPX5, GPX6, GPX7 and GSR antioxydative genes and CDKN1A (p21CIP1) gene was quantified by qRT-PCR (Universal ProbeLibrary, Roche). SDS-PAGE and western-blot experiments were realized to quantify GPx-3 expression and p38 MAPK activation. Flow cytometry studies were performed: (a) to quantify H2O2 level by dichlorodihydrofluorescein diacetate (DCF-DA) staining; (b) to analyze the cell cycle by staining with 7-aminoactinomycin D (7AAD), Alexa Fluor®488-conjugated anti-human Ki67 and Alexa Fluor®488-conjugated anti-phospho-histone H3 (ser10) antibodies; (c) to track the cell divisions with carboxyfluorescein diacetate N-succinimidyl ester (CFSE). Supernatant of MSCs did not modify the GPx-3/H2O2/p38 MAPK axis in KG1a cells. Conversely, when compared with cells in the supernatant of MSCs, adhering KG1a cells were characterized by the exclusive overexpression of GPX3 antioxydative gene, the induction of GPx-3 production, a major decrease in H2O2 concentration and the inactivation of p38 MAPK. These effects were concomitant of cell cycle inhibition: increase in G0 phase, decrease in S and M phase, overexpression of CDKN1A and reduced mitotic activity (CFSE). Altogether these findings suggest that the bone marrow microenvironment plays a key role in the regulation of the oxidative metabolism of leukemic cells by promoting the inhibition of the H2O2/p38 MAPK axis via the induction of GPx-3. Modulation of the GPx-3/H2O2/p38 MAPK pathway by targeting of microenvironmental interactions in leukemia may have clinical relevance and it will be important to verify if these results can be extended in vivo to other models of human leukemias. Disclosures: No relevant conflicts of interest to declare.
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Nègre-Aminou, Pascale, Monique van Erck, Rick E. W. van Leeuwen, John G. Collard, and Louis H. Cohen. "Differential effect of simvastatin on various signal transduction intermediates in cultured human smooth muscle cells11Abbreviations: SMC, smooth muscle cells; S, simvastatin; ERK1/2, extracellular-regulated kinase 1/2; SAPK, stress-activated protein kinase; PDGF, platelet-derived growth factor; PMA, phorbol myristil acetate; POD, peroxydase; MAPK, mitogen-activated protein kinase; TBS, Tris-buffered saline; FPP, farnesyl pyrophosphate; GGPP, geranylgeranyl pyrophosphate; Mev, mevalonic acid lactone, LPA, lysophosphatidic acid; PKC, protein kinase C; GST, glutathione S-transferase; and PAK-CD, p21-activated kinase CRIB domain." Biochemical Pharmacology 61, no. 8 (April 2001): 991–98. http://dx.doi.org/10.1016/s0006-2952(01)00566-4.

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25

"Sélénium plasmatique, teneur en cadmium et activité glutathion peroxydase chez des insuffisants rénaux chroniques." Nutrition Clinique et Métabolisme 2, no. 2 (January 1988): 101. http://dx.doi.org/10.1016/s0985-0562(88)80088-8.

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"Implication du déficit en sélénium et en glutathion peroxydase dans la pathologie de l'hémodialysé." Nutrition Clinique et Métabolisme 2, no. 2 (January 1988): 101. http://dx.doi.org/10.1016/s0985-0562(88)80090-6.

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27

"Détermination de la glutathion peroxydase érythrocytaire par une technique automatisée (Ransel Kit) chez des adultes sains." Nutrition Clinique et Métabolisme 2, no. 2 (January 1988): 95–96. http://dx.doi.org/10.1016/s0985-0562(88)80073-6.

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"Activité de la glutathion peroxydase dans les érythrocytes de nouveaux-nés alimentés par le lait maternel ou artificiel." Nutrition Clinique et Métabolisme 2, no. 2 (January 1988): 104. http://dx.doi.org/10.1016/s0985-0562(88)80100-6.

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"Concentration du sélénium du dialdéhyde malonique et de l'activité glutathion peroxydase dans le plasma d'enfants atteints de neuro, néphro et hépatoblastomes." Nutrition Clinique et Métabolisme 2, no. 2 (January 1988): 98. http://dx.doi.org/10.1016/s0985-0562(88)80083-9.

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"Cinétiques du sélénite de sodium injecté par voie intraveineuse chez le lapin: étude du sélénium et de la glutathion peroxydase plasmatiques." Nutrition Clinique et Métabolisme 2, no. 2 (January 1988): 109. http://dx.doi.org/10.1016/s0985-0562(88)80113-4.

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I, Popov, Manolov IV, Atanasova B, Vasilev V, Dimitrova V, Arabadjieva D, Velkova N, and Yonova D. "Plasma Levels of Selenium (SE) and Glutathion Peroxydase (GSH-PX) and their Relationship to Supplementation of Selenium in Patients with Chronic Renal Failure (CRF) on Hemodialysis (HD)." Archives of Nephrology and Urology 02, no. 01 (2019). http://dx.doi.org/10.26502/anu.2644-2833004.

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"Valeurs du sélénium plasmatique et érythrocytaire (Se), de la glutathion peroxydase (GSH-Px), du malonyldialdéhyde (MDA) et des hydroperoxydes lipidiques en fonction d'une supplémentation séléniée chez 12 enfants phénylcétonuriques." Nutrition Clinique et Métabolisme 2, no. 2 (January 1988): 107. http://dx.doi.org/10.1016/s0985-0562(88)80106-7.

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"Concentration du sélénium dans le sang total et activité érythrocytaire de la glutathion peroxydase chez des sujets sains supplémentés avec du sélénate, sélénite, L-sélénométhionine et de la levure riche en sélénium." Nutrition Clinique et Métabolisme 2, no. 2 (January 1988): 104. http://dx.doi.org/10.1016/s0985-0562(88)80098-0.

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Bouzidi, Nour Elaimane, Samir Borhane Grama, Aboubakeur Essedik Khelef, Duanpeng Yang, and Jian Li. "Inhibition of antioxidant enzyme activities enhances carotenogenesis in microalga Dactylococcus dissociatus MT1." Frontiers in Bioengineering and Biotechnology 10 (September 23, 2022). http://dx.doi.org/10.3389/fbioe.2022.1014604.

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Abstract:
Microalgal biotechnology has become a promising field of research for the production of valuable, sustainable and environmentally friendly byproducts, especially for carotenoids. Bulk accumulation of secondary carotenoids in microalgae are mostly induced by oxidative stress of cells. In this research, we investigated the effects of antioxidant enzyme activity inhibition on carotenogenesis in a microalga Dactylococcus dissociatus MT1. The activities of four major antioxidant enzyme families, namely superoxide dismutase (SOD), catalases (CAT), glutathione peroxydases (GPX) and ascorbate perxodases (APX), were inhibited by relevant inhibitors during the stressed cultivation of D. dissociatus to observe the effects on carotenogensis. A 91% decrease in activity was observed for CAT, comparing with controls without any inhibitors added, followed by 65%, 61%, and 47% for the enzymes SOD, APX, and GPX, respectively. Concomitantly, it was found that this partial inhibition had substantial influences on the accumulation of carotenoids, with the highest production levels obtained in CAT inhibition conditions and an increase of 2.6 times of carotenoid concentration observed, comparing with control cultivation conditions. We conclude that the modulation of antioxidant enzyme activities could lead to the overproduction of carotenoids in this microalgal cell culture, and we expect that this novel approach of optimizing carotenogenesis processes for D. dissociatus cell cultures could be transferrable to other cell culture systems and might have an important impact on the carotenoid production industry.
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