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Journal articles on the topic 'Goats – Embryos'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Karakas, K., H. Alkan, G. Onur, D. Ozen, M. Kaymaz, and H. Izgur. "154 EVALUATION OF EMBRYO RECOVERY RATE AND EMBRYO TRANSFER IN ANGORA GOATS." Reproduction, Fertility and Development 27, no. 1 (2015): 168. http://dx.doi.org/10.1071/rdv27n1ab154.

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The aims of this study were to compare the embryo recovery rate in Angora goats based on application timing; at the beginning (September – October; Group 1) and end (December; Group 2) of the breeding season and to evaluate the viability and survivability of fresh or vitrified-thawed embryos when transferred. For this purpose, nine Angora goats were used as donors and thirthy Angora goats were used as recipients. Donor goats were synchronized and superovulated with traditional protocol and were mated with fertile bucks. At the 156th hour of the mating, embryos were collected surgically and eva

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2

Maia, Ana Lucia R. S., Aline M. Arrais, Lucia Prellwitz, et al. "Embryo development is impaired in goats that are treated for hydrometra and subsequently subjected to superovulation." Veterinary Record 187, no. 10 (2020): e88-e88. http://dx.doi.org/10.1136/vr.105906.

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BackgroundReproductive efficiency after hydrometra (HD) treatment is usually unsatisfactory.MethodsTo identify mechanisms involved in low reproductive efficiency of HD-treated goats, pluriparous dairy goats treated for HD (n=10, HD) or with no reproductive disorders (n=11, control: CONT) were induced to oestrus and superovulated. Goats were mated with fertile bucks and seven days after oestrus, non-surgical embryo recovery was performed. Embryos were evaluated and gene expression was performed.ResultsThere were no differences (P>0.05) in sexual behaviour parameters, superovulation response,

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3

Shin, S. T., S. K. Jang, H. S. Yang, et al. "182LAPAROSCOPIC EMBRYO TRANSFER IN KOREAN BLACK GOATS." Reproduction, Fertility and Development 16, no. 2 (2004): 213. http://dx.doi.org/10.1071/rdv16n1ab182.

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In this study, laparoscopic embryo transfer (ET) was conducted to overcome the disadvantages of laparotomic ET including invasiveness, adhesions and duration of surgery in Korean black goats (Capra hircus aegagrus). Transferred transgenic embryos were produced by DNA pronuclear microinjection of in vivo derived zygotes. Recipient goats were synchronized in estrus by using an intravaginal progesterone device CIDR® for 13 days and injection of 400IU PMSG 48hrs before CIDR® removal. Embryos were transferred on day 4 after CIDR® removal. Recipient goats were starved for 48hrs and suspended head do

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Pellerin, J. L., A. Ashraf, M. Oseikria, et al. "162 CAN CHLAMYDIA ABORTUS BE TRANSMITTED BY EMBRYO TRANSFER IN GOATS?" Reproduction, Fertility and Development 27, no. 1 (2015): 172. http://dx.doi.org/10.1071/rdv27n1ab162.

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Chlamydia abortus is a gram-negative obligate intracellular bacterium. Its lifecycle includes a resistant infectious form and a metabolically active non-infectious form. Chlamydia abortus infection results in abortion in goats; in nonpregnant animals the infection is usually subclinical. Chlamydia abortus presents a major zoonotic risk for pregnant women. The aim of this study was to investigate whether the embryonic zona pellucida (ZP) protects early embryo cells from infection and to test the efficacy of the washing protocol recommended by the IETS for bovine embryos. The study was performed

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Stoltzfus, M., J. Wayman, R. Stilz, and D. Bresnahan. "144 Sex ratio of in vitro-produced goat embryos." Reproduction, Fertility and Development 31, no. 1 (2019): 197. http://dx.doi.org/10.1071/rdv31n1ab144.

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Goats are important livestock species because they produce meat, milk, and fibre and are also easily maintainable on small farms. Although goats provide many products and consumption of goat meat is increasing in the United States, the industry lags compared with many species with regard to IVF techniques to enhance goat production. It has been demonstrated in other species that male IVF embryos tend to develop faster than those of females. This may be due to increased tolerance of male embryos to inadequate conditions, particularly, glucose concentrations in culture media. However, the sex ra

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6

Alsaleh, A., J. L. Pellerin, C. Roux, M. Larrat, G. Chatagnon, and F. Fieni. "166 CAN COXIELLA BURNETII BE TRANSMITTED BY GOAT EMBRYO TRANSFER?" Reproduction, Fertility and Development 25, no. 1 (2013): 231. http://dx.doi.org/10.1071/rdv25n1ab166.

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Coxiella burnetii, an obligate intracellular bacterium of worldwide distribution, is responsible for Q fever. Detection of significant bacterial loads in flushing media and tissue samples (oviducts and uterine horns) from the genital tracts of nonpregnant goats is a risk factor for in utero infection and transmission during embryo transfer (Alsaleh et al. 2011 CIMID 34, 355–360). The aim of this study was to investigate (1) whether cells of early goat embryos isolated from in vivo fertilized goats interact with C. burnetii in vitro, (2) whether the embryonic zona pellucida (ZP) protects early

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Freitas, Vicente J. F., Irina A. Serova, Lyudmila E. Andreeva, et al. "The comparison of two embryo donor breeds for the generation of transgenic goats by DNA pronuclear microinjection." Animal Production Science 54, no. 5 (2014): 564. http://dx.doi.org/10.1071/an13069.

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The aim of the present study was to compare two breeds as embryo donors to produce transgenic goats for the production of human granulocyte colony-stimulating factor. Ten Canindé and 11 Saanen goats were used as donors and received a hormonal treatment for oestrus synchronisation. The superovulation was induced with a total administration of 4.4 mg/kg bodyweight NIH-FSH-P1, given in decreasing doses over 3 days. Donors also received 100 μg of GnRH and they were hand-mated at 36 and 48 h after progestagen removal. Embryo recovery was performed by oviduct flushing at 72 h after progestagen remov

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8

Martinez, A. C., R. C. M. Tramontini, M. A. Saito Jr, C. O. Abreu, and C. R. Alcalde. "153 IN VITRO CULTIVATION OF CAPRINE EMBRYOS PRODUCED IN VIVO." Reproduction, Fertility and Development 22, no. 1 (2010): 235. http://dx.doi.org/10.1071/rdv22n1ab153.

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Independent of embryo stage, when grade III embryos are transferred, they usually provide poor pregnancy rates. The aim of this study was to compare the effect of different in vitro cultivation media on the development of in vivo derived grade III caprine embryos. Twelve adult crossbreed goats from the Animal Breeding and Reproduction Laboratory of Maringá State University were used for this experiment. The goats were not pregnant or lactating. After embryo collection, embryos were classified regarding their development and quality, according to the International Embryo Transfer Society manual

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9

Hall, J., Q. Meng, B. R. Sessions, et al. "29 EFFECT OF EMBRYO CULTURE LENGTH ON PRODUCTION OF CLONED TRANSGENIC GOATS." Reproduction, Fertility and Development 25, no. 1 (2013): 162. http://dx.doi.org/10.1071/rdv25n1ab29.

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The yield of blastocysts and hatched blastocysts using in vitro production (IVP) in goats are still low. The development of caprine embryos is frequently arrested at the 8- to 16-cell stage, indicating suboptimal culture conditions (Jimenez-Macedo et al. 2005 Theriogenology 64, 1249–1262). Our goal was to produce transgenic goats by somatic cell nuclear transfer (SCNT) and further determine whether the length of embryo culture has an effect on development to term. We compared the efficiency of transferring single-cell embryos 12 h post-activation to transferring 4- to 8-cell embryos cultured f

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10

Batt, PA, ID Killeen, and AW Cameron. "Use of single or multiple injections of FSH in embryo collection programmes in goats." Reproduction, Fertility and Development 5, no. 1 (1993): 49. http://dx.doi.org/10.1071/rd9930049.

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The yield of embryos from goats treated with single injections of FSH combined with low doses of PMSG was compared with that from goats treated with conventional superovulatory regimens based on multiple doses of FSH. In one experiment, 101 female goats were assigned to 10 groups. One group received six injections of FSH-P (FSH-P control); a second group received eight injections of Ovagen (Ovagen control). The remaining eight groups conformed to a 2x2x2 factorial design in which goats received a single injection of a low or high dose of either FSH-P or Ovagen in combination with a low or high

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Amorim, L. S., C. A. A. Torres, E. A. M. Amorim, et al. "101 EFFECT OF DIETARY UREA ON EMBRYONIC VIABILITY AND DEVELOPMENT OF TOGGENBURG GOATS." Reproduction, Fertility and Development 21, no. 1 (2009): 151. http://dx.doi.org/10.1071/rdv21n1ab101.

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Urea supplementation in the diet of ruminants may alter the pH and protein concentration in different tissues and may affect the embryo quality and viability. Imbalances in nitrogen metabolism during the periods of oocyte growth and/or fertilization may dispose the zygote, pre-implantation embryo, and surviving conceptus to developmental errors. The aim of this research was to investigate the effect of feeding urea to goats on embryo quality. Eighteen Toggenburg goats, 48.6 ± 7.9 kg of BW, 2.9 ± 0.5 BCS, and 34.3 ± 20.8 months of age, were allocated randomly to 2 treatments: T1 (control, n = 8

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De-Chi, W., H. Jan-Chi, L. Neng-Wen, et al. "255 SONIC HEDGEHOG PROMOTES IN VITRO OOCYTE MATURATION AND EMBRYO DEVELOPMENT IN GOATS." Reproduction, Fertility and Development 27, no. 1 (2015): 217. http://dx.doi.org/10.1071/rdv27n1ab255.

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The signalling of the Hh family peptides is mediated through a cell surface receptor system consisting of 2 proteins: patched (Ptc) and smoothened (Smo). In the absence of Hh ligand, the Hh receptor Ptc represses Smo, whereas in the presence of Hh, the suppression of Smo is lifted, leading to the activation of downstream transcriptional factors (Gli1, Gli2, and Gli3) in vertebrates. Previous studies have examined Sonic hedgehog (Shh) signalling pathways in developing and adult mouse ovaries and concluded that the Shh signalling pathway may be involved in granulosa cell proliferation and oocyte

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Lehloenya, K. C., N. A. Mpebe, and A. Gonzalez-Bulnes. "318 EFFECT OF BREED AND FOLLICULAR STATUS ON RESPONSE TO SUPEROVULATION IN SOUTH AFRICAN GOATS." Reproduction, Fertility and Development 25, no. 1 (2013): 306. http://dx.doi.org/10.1071/rdv25n1ab318.

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This study evaluated the effect of breed and follicular status on quantity and quality of embryos recovered following superovulation in South African goats. Mature, nonlactating Boer (n = 10) and unimproved indigenous (n = 10) goats were used in this trial. Oestrous cycles of does were synchronised with controlled internal drug release (CIDR®; Pfizer Animal Health, New York, NY, USA) dispensers inserted for 9 days and injected with prostaglandin at CIDR insertion. All does were superovulated with pFSH, administered in 7 dosages, at 12 h intervals, starting from 48 h before CIDR removal. Ultras

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Buzzell, N., S. Blash, K. Miner, et al. "94 Impact of number of embryos transferred on the number of offspring produced in a commercial transgenic founder production operation." Reproduction, Fertility and Development 31, no. 1 (2019): 173. http://dx.doi.org/10.1071/rdv31n1ab94.

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The production of transgenic founder dairy goats (cross-bred Saanens, Alpines, Toggenburgs, and Nubians) involves the collection, microinjection, and transfer of numerous embryos into suitable recipient goats to ultimately produce a transgenic founder(s). The objective of this study was to determine the most efficient number of microinjection embryos to transfer to suitable recipients for transgenic founder generation. This is critically important in a commercial production program, as it impacts the goal for the number of embryos collected from donors, number of recipients utilised, and, henc

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15

Deng, Mingtian, Zifei Liu, Caifang Ren, Shiyu An, Yongjie Wan, and Feng Wang. "Highly methylated Xist in SCNT embryos was retained in deceased cloned female goats." Reproduction, Fertility and Development 31, no. 5 (2019): 855. http://dx.doi.org/10.1071/rd18302.

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X (inactive)-specific transcript (Xist) is crucial in murine cloned embryo development, but its role in cloned goats remains unknown. Therefore, in this study we examined the expression and methylation status of Xist in somatic cell nuclear transfer (SCNT) embryos, as well as in ear, lung, and brain tissue of deceased cloned goats. First, the Xist sequence was amplified and a differentially methylated region was identified in oocytes and spermatozoa. Xist methylation levels were greater in SCNT- than intracytoplasmic sperm injection-generated female 8-cell embryos. In addition, compared with n

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16

Gibbons, A. E., F. Pereyra-Bonnet, and M. I. Cueto. "92 CRYOTIPS: A SIMPLE VITRIFICATION TECHNIQUE FOR SHEEP AND GOAT EMBRYOS." Reproduction, Fertility and Development 22, no. 1 (2010): 205. http://dx.doi.org/10.1071/rdv22n1ab92.

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Vitrification procedure for embryos has not been used massively because there is not a standard protocol according to species. We evaluated reproductive efficiency of vitrified embryos in plastic tips of micropipettes. Estrous cycles of 36 Criolla goats (donors, n = 10; recipients, n = 26) and 48 Merino sheep (donors, n = 10; recipients, n = 38) were synchronized by sponges containing 60mgof MAP (Progespon®). At sponge removal (goats, Day 18; sheep, Day 14), recipients received 200 UI of eCG (Novormon®, Syntex, Argentina). Sheep and goat donors were superovulated with a total of 80 (igpFSH (Fo

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Cueto, M. I., J. Fernandez, M. M. Bruno-Galarraga, F. Pereyra-Bonnet, and A. Gibbons. "196 Fertilization Rate in Superovulated Criolla Goats Following Artificial Insemination or Natural Mating." Reproduction, Fertility and Development 30, no. 1 (2018): 238. http://dx.doi.org/10.1071/rdv30n1ab196.

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The Criolla breed is a local genetic resource, distributed in Patagonia, Argentina, whose primary production is meat. In the Criolla goat, efforts were made to locate productively superior males and to conserve their genetic material. Studies were carried on the feasibility of obtaining superior offspring through the implementation of embryo transfer programs. We assessed the fertilization rate and embryo production following AI with frozen semen or natural mating in Criolla goats subjected to a superovulation program. During the breeding season (May, 41° S), 26 Criolla goats were treated for

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NAGASHIMA, Hiroshi, Kanji MATSUI, Toru SAWASAKI, and Yasuhiko KANO. "Nonsurgical Collection of Embryos in Shiba Goats." Experimental Animals 36, no. 1 (1987): 51–56. http://dx.doi.org/10.1538/expanim1978.36.1_51.

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19

Yuan, Yu-Guo, Liyou An, Baoli Yu, et al. "Expression of Recombinant Human Alpha-Lactalbumin in the Milk of Transgenic Goats Using a Hybrid Pomoter/Enhancer." Journal of Analytical Methods in Chemistry 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/281031.

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To improve nutrient content of goat milk, we describe the construction of a vector (pBLAC) containing a hybrid goatβ-lactoglobulin (BLG) promoter/cytomegalovirus (CMV) enhancer. We also describe the generation of transgenic goats expressing rhLA by somatic cell nuclear transfer (SCNT). Of 334 one-cell stage embryos derived from three transgenic cell lines and 99 embryos derived from non-transgenic (NT) cells surgically transferred to the oviducts of 37 recipients, two recipients delivered two kids (2%) from the non-transfected line and five recipients delivered six kids (1.8%) from transgenic

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Batt, PA, DK Gardner, and AW Cameron. "Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro." Reproduction, Fertility and Development 3, no. 5 (1991): 601. http://dx.doi.org/10.1071/rd9910601.

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The effect of oxygen concentration and the source of protein in culture medium on the development of 2- to 4-cell goat embryos in vitro was investigated. Embryos were collected from superovulated Angora-Cashmere-cross goats 48 h after ovulation and cultured for 6 days in synthetic oviduct fluid (SOF) medium under one of two oxygen concentrations (20% or 7%) and in the presence of one of five protein sources; Miles bovine serum albumin (Miles BSA), Commonwealth Serum Laboratory bovine serum albumin (CSL BSA), goat serum (GS), fetal calf serum (FCS) and human serum (HS). In the presence of 20% o

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Feltrin, C., N. Mohamad-Fauzi, S. Gaudencio Neto, et al. "30 EFFECT OF THE CYTOPLAST SOURCE AND KARYOPLAST TYPE ON THE DEVELOPMENT OF HANDMADE CLONED EMBRYOS IN GOATS." Reproduction, Fertility and Development 24, no. 1 (2012): 127. http://dx.doi.org/10.1071/rdv24n1ab30.

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The aim of this study was to compare the effect of 2 donor cell types (bone marrow-derived mesenchymal stem cells, BM-MSC and skin fibroblast cells, SFC) and the source of oocytes (in vivo- and in vitro-matured goat oocytes) on the developmental capacity of handmade cloned goat embryos, following our procedures adapted from cattle (Ribeiro et al., 2009, Cloning Stem Cells 11, 377–386). In vivo- and in vitro-matured oocytes obtained postmortem from 36 superovulated and 90 nonstimulated goats were used as cytoplasts for cloning, after 26 h from the induction of ovulation or 20 h from the onset o

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Wells, D. N., M. C. Berg, S. A. E. Cole, et al. "31 EFFECT OF ACTIVATION METHOD ON IN VIVO DEVELOPMENT FOLLOWING SOMATIC CELL NUCLEAR TRANSFER IN GOATS." Reproduction, Fertility and Development 24, no. 1 (2012): 127. http://dx.doi.org/10.1071/rdv24n1ab31.

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The effects of activation method and timing between fusion and activation in goat somatic cell nuclear transfer (NT) were investigated. In vivo-ovulated oocytes were surgically flushed from donors 54 to 62 h after CIDR withdrawal in the breeding season and enucleated after brief ultraviolet exposure. Transfected fibroblasts and epithelial cells from 4 clonal strains were serum-starved for 4 days before NT. Two direct current electric pulses (2 kV cm–1 each for 10 μs) were used to induce fusion and simultaneous activation. Forty-five minutes after successful fusion, reconstructs received a seco

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Baldassarre, H., K. M. Rao, N. Neveu, et al. "Laparoscopic ovum pick-up followed by in vitro embryo production for the reproductive rescue of aged goats of high genetic value." Reproduction, Fertility and Development 19, no. 5 (2007): 612. http://dx.doi.org/10.1071/rd07024.

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The efficiency of laparoscopic ovum pick-up (LOPU) followed by in vitro embryo production (IVEP) in the propagation of aged goats with poor reproductive performance was evaluated in the present study. Follicular development was stimulated in donor goats with 80 mg follicle-stimulating hormone and 300 IU equine chorionic gonadotrophin administered 36 h before LOPU. In addition, goats were heat synchronised with intravaginal sponges containing 60 mg medroxyprogesterone acetate for 10 days and a luteolytic injection of 125 µg cloprostenol 36 h before sponge removal and LOPU. Following in vitro ma

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Yong, Zhang, and Li Yuqiang. "Nuclear-Cytoplasmic Interaction and Development of Goat Embryos Reconstructed by Nuclear Transplantation: Production of Goats by Serially Cloning Embryos1." Biology of Reproduction 58, no. 1 (1998): 266–69. http://dx.doi.org/10.1095/biolreprod58.1.266.

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Selgrath, J. P., M. A. Memon, T. E. Smith, and K. M. Ebert. "Collection and transfer of microinjectable embryos from dairy goats." Theriogenology 34, no. 6 (1990): 1195–205. http://dx.doi.org/10.1016/s0093-691x(05)80018-4.

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Van Soom, A., A. E. Wrathall, A. Herrler, and H. J. Nauwynck. "Is the zona pellucida an efficient barrier to viral infection?" Reproduction, Fertility and Development 22, no. 1 (2010): 21. http://dx.doi.org/10.1071/rd09230.

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Although the transfer of embryos is much less likely to result in disease transmission than the transport of live animals, the sanitary risks associated with embryo transfer continue to be the subject of both scientific investigations and adaptations of national and international legislation. Therefore, the implications are important for veterinary practitioners and livestock breeders. In vivo-derived and in vitro-produced embryos are widely used in cattle and embryos from other species, such as sheep, goats, pigs and horses, are also currently being transferred in fairly significant numbers.

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Melican, D., R. Butler, N. Hawkins, et al. "58ESTRUS SYNCHRONIZATION OF DAIRY GOATS UTILIZED AS RECIPIENTS FOR CAPRINE NUCLEAR TRANSFER EMBRYOS." Reproduction, Fertility and Development 16, no. 2 (2004): 151. http://dx.doi.org/10.1071/rdv16n1ab58.

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The timing of estrus synchrony between donor and recipient does is an important consideration in an embryo transfer program. Experiments were conducted to determine the optimal time of estrus synchrony between donor and recipient dairy goats used in a commercial nuclear transfer (NT) program. Donor and recipient synchronization was achieved by implanting either a 3-mg norgestomet ear implant (Crestar®, Intervet Int. B.V., Boxmeer, Holland) or a 300-mg progesterone vaginal implant (CIDR-G®, Pharmacia and Upjohn Ltd. Co., Auckland, NZ) on Day 0. A single 5mg intramuscular injection of prostaglan

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Wang, Bin, H. Baldassarre, J. Pierson, F. Cote, K. M. Rao, and C. N. Karatzas. "The in vitro and in vivo development of goat embryos produced by intracytoplasmic sperm injection using tail-cut spermatozoa." Zygote 11, no. 3 (2003): 219–27. http://dx.doi.org/10.1017/s0967199403002260.

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The objective of this study was to assess the efficacy of a novel intracytoplasmic sperm injection (ICSI) procedure, as well as the in vitro and in vivo developmental competence of goat embryos produced by ICSI. Oocyte-cumulus complexes recovered by LOPU from donors stimulated with gonadotrophins were matured in vitro. Fresh goat semen was used for ICSI following Percoll gradient washing. Tail-cut spermatozoa were microinjected into the ooplasm of goat oocytes using a piezo micropipette-driving system (PiezoDrill). In order to assess developmental competence, the ICSI-derived zygotes were cult

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Buzzell, N., S. Blash, K. Miner, et al. "194 Superovulation response does not affect embryo development of pronuclear microinjected embryos in the goat." Reproduction, Fertility and Development 31, no. 1 (2019): 221. http://dx.doi.org/10.1071/rdv31n1ab194.

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Superovulation of donor animals is essential in the production of transgenic founder goats generated through microinjection. There can be a marked variable response to the exogenous hormones used for superovulation. The objective of this study was to examine how the superovulatory response of individual goats affected the ability of the fertilized, microinjected embryos to develop into offspring. The donors were superovulated using a progesterone implant on Day 0, a prostaglandin injection at Day 7, 2 injections ~12h apart of 32 to 36mg of FSH on Day 12 to 15, progesterone implant removal on D

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El-Gayar, M., A. N. Al Yacoub, M. Gauly, and W. Holtz. "72 SUPERIOR PREGNANCY RATES WITH THE TRANSFER OF OPEN PULLED STRAW-VITRIFIED V. CONVENTIONALLY CRYOPRESERVED EMBRYOS IN GOATS." Reproduction, Fertility and Development 23, no. 1 (2011): 141. http://dx.doi.org/10.1071/rdv23n1ab72.

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Two separate studies were conducted in the course of which goat embryos were cryopreserved either by the open pulled straw (OPS) procedure of (Vajta et al. 1998 Mol. Reprod. Dev. 51, 53–58) or by conventional slow freezing. Embryos of each doe were assigned to both methods. A total of 94 morphologically intact blastocysts, collected by transcervical flushing from superovulated pluriparous Boer goat does, were involved. For OPS-vitrification the method of Vajta et al. (1998) was slightly modified (El-Gayar et al. 2008 Cryobiology 57, 191–194). It involves exposure of the embryos to a medium con

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Jia, Ruoxin, Guomin Zhang, Yixuan Fan, et al. "MBD1 and MeCP2 expression in embryos and placentas from transgenic cloned goats." Zygote 25, no. 4 (2017): 462–71. http://dx.doi.org/10.1017/s0967199417000284.

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SummaryDNA methylation is an important form of epigenetic regulation in mammalian development. Methyl-CpG-binding domain protein 1 (MBD1) and methyl-CpG-binding domain protein 2 (MeCP2) are two members of the MBD subfamily of proteins that bind methylated CpG to maintain the silencing effect of DNA methylation. Given their important roles in linking DNA methylation with gene silencing, this study characterized the coordinated mRNA expression and protein localization of MBD1 and MeCP2 in embryos and placentas and aimed to analysis the effects of MBD1 and MeCP2 on transgenic cloned goats. Our re

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Matsas, D., V. Huntress, H. Levine, et al. "90 PRESERVATION OF HERITAGE LIVESTOCK BREEDS: INTEGRATED PROGRAM TO CRYOPRESERVE GERMPLASM FROM TENNESSEE MYOTONIC GOATS." Reproduction, Fertility and Development 17, no. 2 (2005): 195. http://dx.doi.org/10.1071/rdv17n2ab90.

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The genetic diversity of common commercial livestock has diminished precipitously due to intensive selection by agribusiness. Rare and/or heritage breeds of domestic livestock are thought to be of low commercial value, yet such breeds contain unique genes that impart valuable traits including disease and parasite resistance, efficient feed conversion, high fecundity, and unique food and fiber qualities. These irreplaceable genetics can be preserved by the establishment of a comprehensive germplasm preservation program whereby the complete genetics of a given unimproved breed can be reestablish

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Baldassarre, Hernan, Bin Wang, Melanie Gauthier, Nathalie Neveu, Anthoula Lazaris, and Costas N. Karatzas. "Effect of GnRH injection timing in the production of pronuclear-stage zygotes used for DNA microinjection." Zygote 12, no. 3 (2004): 257–61. http://dx.doi.org/10.1017/s0967199404002849.

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This study was aimed at developing a hormonal treatment protocol in order to optimize the proportion of pronuclear-stage embryos to be used for DNA microinjection in a goat transgenic founder production programme. A total of 46 adult BELE® and 47 adult standard goats (1–5 years old) were used as donors and recipients, respectively. They were heat-synchronized using intravaginal sponges containing 60 mg medroxyprogesterone acetate for 10 days with an injection of 125 μg cloprostenol on the morning of the eighth day. Recipients were injected with 400 IU eCG at the time of sponge removal while do

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Yong, Zhang, Qian Jufen, Wang Jianchen, Wang Guangya, and Ma Baohua. "Bisection of embryos and production of monozygotic twins in goats." Theriogenology 29, no. 1 (1988): 338. http://dx.doi.org/10.1016/0093-691x(88)90166-5.

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Singh, Mahipal, Ki-Eun Park, William Tyler та ін. "PSXIII-40 Superovulation and oocyte recovery rates in CIDR synchronized goats treated with PGF2α, FSH and GnRH during breeding season". Journal of Animal Science 97, Supplement_3 (2019): 367. http://dx.doi.org/10.1093/jas/skz258.732.

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Abstract Embryo transfer is an advanced technology and has great potential in increasing desired livestock without physical boundaries. Multiple embryos can be produced in-vitro or in-vivo using semen from desired males. Early stage embryos (zygotes) are also ideal tools for genome-editing to alter the desired traits. However, the small number of mature oocytes produced each cycle is a major limitation of this technology. Scientists have used drugs to stimulate follicles to increase the number of oocytes/embryos. Extra oocytes/embryos produced can be cryopreserved or shipped to distant locatio

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Rodríguez-Alvarez, Lleretny, José Cox, Felipe Navarrete, et al. "Elongation and gene expression in bovine cloned embryos transferred to temporary recipients." Zygote 17, no. 4 (2009): 353–65. http://dx.doi.org/10.1017/s0967199409005486.

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SummaryElongated embryos provide a unique source of information about trophoblastic differentiation, gene expression and maternal-embryonic interactions; however they are difficult and costly to obtain, especially elongated cloned embryos. One alternative is their production in heterologous temporary recipients such as sheep and goats. We aimed to produce elongated bovine cloned embryos using heterologous transfer to temporary recipients. Day-7 cloned cattle blastocysts were transferred to the uteri of ewes and goats and recovered as elongated structures at day 17. We evaluated elongation, len

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Zang, Xupeng, Chen Zhou, Wenjing Wang, et al. "Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats." Biomolecules 11, no. 3 (2021): 472. http://dx.doi.org/10.3390/biom11030472.

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Endometrial receptivity represents one of the leading factors affecting the successful implantation of embryos during early pregnancy. However, the mechanism of microRNAs (miRNAs) to establish goat endometrial receptivity remains unclear. This study was intended to identify potential miRNAs and regulatory mechanisms associated with establishing endometrial receptivity through integrating bioinformatics analysis and experimental verification. MiRNA expression profiles were obtained by high-throughput sequencing, resulting in the detection of 33 differentially expressed miRNAs (DEMs), followed b

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Monniaux, Danielle, Gérard Baril, Anne-Lyse Laine, et al. "Anti-Müllerian hormone as a predictive endocrine marker for embryo production in the goat." REPRODUCTION 142, no. 6 (2011): 845–54. http://dx.doi.org/10.1530/rep-11-0211.

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Recently, we demonstrated the relationship between anti-Müllerian hormone (AMH) circulating concentrations, ovarian follicles, and embryo production in cattle. However, they have not yet been established in a species with a seasonal breeding activity. Thus, goats were subjected to repeated in vivo embryo production during the breeding season, at the end of the breeding season, and at the end of the anestrus season. Embryo production after FSH treatment was highly repeatable for each goat. Plasma AMH concentrations, measured before the first FSH treatment, were highly correlated with the number

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Souza-Fabjan, Joanna M. G., Lucas F. L. Correia, Ribrio I. T. P. Batista, Yann Locatelli, Vicente J. F. Freitas, and Pascal Mermillod. "Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats." Animals 11, no. 3 (2021): 873. http://dx.doi.org/10.3390/ani11030873.

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Reproductive seasonality may have a considerable influence on the efficiency of assisted reproductive technologies in seasonal species. This study evaluated the effect of season on cleavage, blastocyst rates and quality of in vitro produced (IVP) goat embryos. In total, 2348 cumulus–oocyte complexes (COCs) were recovered from slaughterhouse ovaries and subjected to the same IVP system throughout 1.5 years (49 replicates). The odds ratio (OR) among seasons was calculated from values of cleavage and blastocyst rates in each season. Cleavage rate was lower (p < 0.05) in spring (anestrus), in c

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Neto, L. M. Freitas, M. H. B. Santos, C. R. Aguiar Filho, et al. "Reliability of ultrasound for early sexing of goat fetuses derived from natural mating and from fresh, frozen and vitrified embryo transfer." Reproduction, Fertility and Development 22, no. 2 (2010): 489. http://dx.doi.org/10.1071/rd09155.

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The aim of the present study was to identify the migration period of the genital tubercle and its later differentiation into external genital structures in fetuses derived from natural mating and fetuses from fresh, frozen and vitrified embryo transfer. A transrectal ultrasound with a double-frequency linear transducer (6.0 and 8.0 MHz) was used to monitor 123 goat fetuses, which were allocated to one of four groups: fetuses originating from controlled natural mating (G1, n = 32) and fetuses derived from fresh (G2, n = 34), frozen (G3, n = 30) and vitrified (G4, n = 27) embryo transfer. The tr

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Gonz´lez-Bulnes, Antonio, David T. Baird, Bruce K. Campbell, et al. "Multiple factors affecting the efficiency of multiple ovulation and embryo transfer in sheep and goats." Reproduction, Fertility and Development 16, no. 4 (2004): 421. http://dx.doi.org/10.1071/rd04033.

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This review offers an overview of the basic characteristics of in vivo embryo technologies, their current status, the main findings and the advances gained in recent years, and the outstanding subjects for increasing their efficiency. The use of superovulation and embryo transfer procedures remains affected by a high variability in the ovulatory response to hormonal treatment and by a low and variable number of transferable embryos and offspring obtained. This variability has been classically identified with both extrinsic (source, purity of gonadotrophins and protocol of administration) and i

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Leoni, Giovanni Giuseppe, Sara Succu, Valentina Satta, et al. "In vitro production and cryotolerance of prepubertal and adult goat blastocysts obtained from oocytes collected by laparoscopic oocyte-pick-up (LOPU) after FSH treatment." Reproduction, Fertility and Development 21, no. 7 (2009): 901. http://dx.doi.org/10.1071/rd09015.

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This study compares the developmental capacity and cryotolerance of embryos produced from oocytes of stimulated prepubertal and adult Sarda goats. Twelve prepubertal and 13 adult goats were each given 110 and 175 IU FSH, respectively, and cumulus–oocyte complexes (COCs) were collected by laparoscopic oocyte-pick-up (LOPU). After in vitro maturation, fertilisation and culture (IVMFC), blastocysts were vitrified, warmed and blastocoel re-expansion and gene expression were evaluated. Prepubertal goats produced a higher COCs number than adults (mean ± s.e.m., 89.67 ± 5.74 and 26.69 ± 3.66, respect

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García-León, R. A., E. Flórez-Solano, and B. D. Santiago-Bayona. "Designing a stretcher artificial insemination, collection and transfer of embryos goats." Journal of Physics: Conference Series 1409 (November 2019): 012011. http://dx.doi.org/10.1088/1742-6596/1409/1/012011.

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Pereira, P. V., L. Correia, R. Batista, et al. "82 Invitro embryo production outcomes in adult goats is affected by season." Reproduction, Fertility and Development 33, no. 2 (2021): 149. http://dx.doi.org/10.1071/rdv33n2ab82.

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In seasonal breeders, reproductive seasonality can have a substantial influence on the efficiency of assisted reproductive technologies. This study assessed seasonal effects on cleavage and blastocyst rates, as well as on quality of invitro-produced (IVP) goat embryos over 18 months. In total, 2348 (autumn: 811, spring: 404, summer: 639, and, winter: 494) cumulus–oocyte complexes (COC) were recovered from slaughterhouse ovaries during 49 replicates (autumn: 17, spring: 7, summer: 15, and, winter: 10) and matured in TCM-199 with 10ng mL−1 epidermal growth factor and 100µM cysteamine for 22h at

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Pereira, P. V., L. Correia, R. Batista, et al. "82 Invitro embryo production outcomes in adult goats is affected by season." Reproduction, Fertility and Development 33, no. 2 (2021): 149. http://dx.doi.org/10.1071/rdv33n2ab82.

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In seasonal breeders, reproductive seasonality can have a substantial influence on the efficiency of assisted reproductive technologies. This study assessed seasonal effects on cleavage and blastocyst rates, as well as on quality of invitro-produced (IVP) goat embryos over 18 months. In total, 2348 (autumn: 811, spring: 404, summer: 639, and, winter: 494) cumulus–oocyte complexes (COC) were recovered from slaughterhouse ovaries during 49 replicates (autumn: 17, spring: 7, summer: 15, and, winter: 10) and matured in TCM-199 with 10ng mL−1 epidermal growth factor and 100µM cysteamine for 22h at

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Matsas, D., V. Huntress, H. Levine, G. Saperstein, and E. W. Overstrom. "108CRYOPRESERVATION OF GERMPLASM FROM HERITAGE BREEDS OF DOMESTIC LIVESTOCK: SEASONAL EFFECTS ON SUPEROVULATION RESPONSE AND EMBRYO PRODUCTION." Reproduction, Fertility and Development 16, no. 2 (2004): 176. http://dx.doi.org/10.1071/rdv16n1ab108.

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There is an urgent need to preserve the genetic diversity of rare breeds of domestic livestock. However, it is unknown if conventional superovulation methods are effective in unimproved breeds. The establishment of germplasm cryostorage banks requires the ability to produce viable preimplantation stage embryos. To our knowledge, we describe here the first successful production and cryopreservation of embryos from Gulf Coast Native (GCN) sheep and Tennessee Myotonic (TM) goats. Mature GCN ewes and TM does were synchronized (prostaglandin F2α, 7.5–10mg i.m.) and all TM does, and some GCN ewes, w

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Abonyi, F. O., O. E. Ikejiofor, and K. I. Idika. "Foetal wastage in food animals slaughtered at Nsukka Municipal abattoir, Nigeria." Nigerian Journal of Animal Production 41, no. 1 (2021): 205–12. http://dx.doi.org/10.51791/njap.v41i1.2706.

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This study was conducted to evaluate foetal/pregnancy losses following slaughtering of food animals at Nsukka Municipal Abattoir Enugu State, Nigeria over a period of three months (April to June). The pregnancy status of each animal was determined tentatively by visual observation and by palpation of the exposed uterus after slaughtering. Attempts were made to recover embryos from dams with structures suggestive of pregnancy by flushing. Foetuses were recovered from slaughtered cattle, goats and pigs. The ages of recovered foetuses were determined and grouped according to the stage of gestatio

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Fu, M. Z., G. Li та Z. Q. Zhou. "Polymorphisms of LHβ and GnRHR genes and their association with the number of embryos recovered in goats". Animal Production Science 54, № 8 (2014): 987. http://dx.doi.org/10.1071/an13076.

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The objective of the present study was to explore a predictor of superovulation response on the basis of associations between the number of embryos recovered and gene polymorphism. Variation in the goat LHβ and GnRHR genes was investigated using polymerase chain reaction–single-strand conformational polymorphism and DNA sequencing. Two single nucleotide polymorphisms (SNPs) were identified in the 5′-UTR of LHβ gene (A59C, P1 locus) and in the Exon 2 of GnRHR gene (T177A, P6 locus). At the P1 locus in both breeds, the frequencies of one allele were 0.46 and 0.51, respectively. At the P6 locus,

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Apimeteeumrong, M., A. Thuangsanthia, N. Leingchaloen, et al. "25 THE EFFECTS OF OOCYTE SOURCES AND ACTIVATION PROTOCOLS ON THE DEVELOPMENT OF CLONED GOAT EMBRYOS." Reproduction, Fertility and Development 19, no. 1 (2007): 131. http://dx.doi.org/10.1071/rdv19n1ab25.

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The objective of this study was to compare the development to the morula and blastocyst stages, after either cycloheximide (CHX) or ethanol (ETOH) activation, in somatic nuclear transfer (NT) goat embryos derived from 2 sources of oocytes. In vivo- and in vitro-matured oocytes were obtained from FSH-stimulated goats (Native, Saanen, and Native-Saanen crossbred goats). Gonadotropin treatment was performed with a modified program of a previous report (Reggio et al. 2001 Biol. Reprod. 64, 849-856). In vivo-matured oocytes were flushed from the oviduct of donor goats by exposing the reproductive t

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Cognié, Yves, Nati Poulin, Yann Locatelli, and Pascal Mermillod. "State-of-the-art production, conservation and transfer of in-vitro-produced embryos in small ruminants." Reproduction, Fertility and Development 16, no. 4 (2004): 437. http://dx.doi.org/10.1071/rd04029.

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Today, although not efficient enough to replace multiple ovulation and embryo transfer, in vitro embryo production for small ruminants is a platform for new reproductive technologies, such as embryo sexing, transgenesis and cloning. The in vitro embryo-production system developed for sheep and goats is more efficient now than 15 years ago, but could still be improved. Laparoscopic collection of oocytes in live animals treated with gonadotrophin indicates a promising future for the application of this technology to genetic improvement programmes. Oocyte maturation in defined medium with epiderm

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