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Dissertations / Theses on the topic 'Gonadotrophin secretion'

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Published: 4 June 2021

Last updated: 10 February 2022

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1

Honaramooz, Ali. "Neuroendocrinology of gonadotrophin secretion in prepubertal heifers." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ37889.pdf.

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2

Durnin, Anne Theresa. "Secretory heterogeneity among anterior pituitary gonadotrophs." Thesis, University of Oxford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358632.

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3

Wood, Sara C. "Ovarian regulation of pituitary gonadotrophin secretion in domestic ruminants." Thesis, University of Reading, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333527.

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4

Buckler, Helen Margaret. "Gonadotrophin, inhibin and sex steroid secretion in disorders of ovulation." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305188.

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5

Dodson, S. E. "Reproductive endocrinology of the heifer from birth to the peripubertal period." Thesis, University of Nottingham, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376401.

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6

Lewis, C. E. "Factors involved in control of gonadotrophin secretion by the anterior pituitary gland." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375271.

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7

Forsdike, Rachel Anne. "In utero development of sexually dimorphic gonadotrophin-releasing hormone (GnRH) secretion in sheep." Thesis, University of Cambridge, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620917.

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8

Castillo, R. J. "Bioassay and isolation of bovine ovarian inhibin and its effects on gonadotrophin secretion." Thesis, University of Reading, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233158.

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9

Evans, Neil Price. "The regulation of gonadotrophin secretion following divergent selection for pituitary responsiveness to GnRH." Thesis, University of Edinburgh, 1991. http://hdl.handle.net/1842/19730.

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Divergent selection based on the LH response to a 5μg dose of GnRH, has created two lines of sheep which differ in their ability to release gonadotrophins in response to a GnRH challenge in both male and female lambs. Significant correlated between line differences have also been reported in female reproductive performance. The aim of this project was to investigate the regulation of gonadotrophin secretion in animals from the two lines, and to elucidate the primary site of the selected difference/s. Physiological studies of adult ewes and prepubertal ram lambs demonstrated that despite similar peripheral steroid concentrations, endogenous and exogenously stimulated gonadotrophin secretion differed significantly between the two lines. Mean LH and FSH concentrations in the prepubertal male lambs were significantly higher in the High line than the Low line, due to the secretion of LH pulses of significantly greater amplitude by the High line ram lambs. Similarly, higher amplitude LH pulses were observed in the High line ewes during the follicular phase of the oestrous cycle. The age related changes in basal LH secretion in the ram lambs and the observation of significant differences in LH pulse amplitude in the adult ewes during the follicular phase of the oestrous cycle, when progesterone negative feedback is reduced, indicate that the effects of the between line difference in the regulation of endogenous LH secretion are regulated by gonadal negative feedback. However studies in prepubertal ram lambs demonstrated that the primary site of the selected difference was at the level of the hypothalamo/pituitary gland complex. Studies of the regulation of LH secretion by the hypothalamo/pituitary gland complex demonstrated that the High line lambs appeared to secrete significantly less GnRH than the Low line and that the pituitary glands of the High line were 5 fold more sensitive to GnRH than the Low line. Pituitary sensitivity encompasses a large number of variables, including gonadotrophe and GnRH receptor number, the intracellular events which follow receptor activation and the amount of releasable LH stored in the pituitary gland, the individual or combined effects of which could result in differences in pituitary sensitivity. Pituitary gonadotrophe number/size was studied indirectly as a function of pituitary gland weight. The pituitary glands obtained from the High line tended to be heavier than those obtained from the Low line, however this difference was not statistically significant. The pituitary glands of the High line were also found to contain significantly more GnRH receptors/mg of protein than the Low line. The importance of this difference with regard to pituitary sensitivity was questioned, however, following the demonstration that the between line difference in the magnitude of the LH response was maintained <i>in vitro</i> following either GnRH stimulated LH release or the direct stimulation of both the Ca^2+-calmodulin and Protein Kinase C second messenger systems. Examination of the pituitary stores of LH in the two lines demonstrated that the 5μg dose of GnRH used in the selection programme stimulated a maximal release of LH in both lines, and that the High line stored significantly greater quantities of releasable LH compared with the Low line. The results also indicated that the two lines may differ in their ability to synthesise LH in response to GnRH stimulation.

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10

Lujan, Marla Elaine. "The effects of stimulating endogenous corticotrophin-releasing hormone on gonadotrophin secretion in rhesus monkeys." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ63331.pdf.

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11

Mann, George Edward. "The role of inhibin and oestradiol in the control of gonadotrophin secretion in the ewe." Thesis, University of Edinburgh, 1991. http://hdl.handle.net/1842/19978.

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The main aims of the studies described in this thesis were firstly to determine the source of inhibin from the ovary of the sheep, and secondly to investigate the physiological role of inhibin in the control of gonadotrophin production, particularly that of FSH. The source of ovarian inhibin production was investigated by measuring inhibin secretion directly from the ovary <i>in vivo</i>, and by individual follicles <i>in vitro</i>. Inhibin secretion did not differ between animals at different stages of the luteal and follicular phase of the oestrous cycle. The secretion rate of inhibin was unaffected by the presence or absence of luteal tissue leading to the suggestion that, in the sheep, the corpus luteum does not produce significant quantities of inhibin. The results of these studies indicated that, like oestradiol, the majority of inhibin is produced by large (≥3mm) antral follicles. However, while most oestradiol was secreted by the large oestrogenic follicle(s), a significant amount of inhibin was also produced by large non-oestrogenic atretic follicles and by small antral follicles. A series of experiments involving passive immunisation against inhibin and/or oestradiol were then undertaken to investigate the relative importance of these two hormones in the control of gonadotrophin production. Peripheral LH concentrations were unaffected by immunisation against inhibin, and in a further experiment administration of inhibin in 'steroid stripped' ovine follicular fluid was shown to have no effect on the timing or magnitude of the oestradiol benzoate-induced LH surge in ovariectomised ewes, or on the concentration of LH following acute ovariectomy. In the passive immunisation studies, injection of antibodies to inhibin or oestradiol resulted in a highly significant, though transitory rise in the peripheral plasma concentration of FSH during both the luteal and follicular phases of the oestrus cycle, while combined immunisation against both hormones resulted in a significantly larger rise in FSH concentration of similar size to that seen following acute ovariectomy. Furthermore, treatment with physiological quantities of inhibin or oestradiol was found to partly prevent the rise in FSH concentration seen following acute ovariectomy, while a combined treatment with both hormones completely prevented this rise. Finally, immunisation against inhibin or oestradiol was shown to cause a large increase in the number of follicles per ovary, resulting in an increase in ovarian inhibin secretion following immunisation against oestradiol, and an increase in ovarian oestradiol secretion following immunisation against inhibin. These results indicate that inhibin plays an important physiological role in the control of FSH secretion during both the luteal and follicular stages of the sheep oestrous cycle, and suggest that inhibin and oestradiol act together in the control of FSH secretion.

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12

Chairil, Redna Auroret. "The effect of chicken luteinizing-hormone releasing-hormone (LHRH) -I and -II on gonadotrophin secretion in domestic fowl." Thesis, University of Reading, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307005.

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13

Law, Andrew Stephen. "The role of follicular fluid proteins in the control of gonadotrophin secretion and follicular development in the heifer." Thesis, University of Edinburgh, 1991. http://hdl.handle.net/1842/19914.

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Previous studies in this and other laboratories have, to date, failed to result in the development of a commercially useful technique for the reliable induction of twinning in cattle. The use of methods known to be efficacious in sheep have not resulted in repeatable effects in the cow. It would appear that the cow differs from the sheep in some important aspect of the control of the reproductive processes, particularly those governing follicular growth and dominance. The aim of these studies was to clarify the effects of follicular fluid proteins on follicular growth and gonadotrophin secretion. A previous study in this laboratory (Price, 1987) demonstrated that LH and FSH concentrations were grossly elevated following immunisation against a porcine follicular fluid preparation. We sought to confirm this observation and extend our understanding of the underlying processes involved. Heifers immunised against an ovine follicular fluid preparation displayed abnormally elevated peripheral FSH and LH concentrations. However, no difference in the response of heifers to an exogenous bolus of GnRH during the luteal phase of the oestrous cycle was observed. Analysis of the patterns of gonadotrophin secretion during the luteal and follicular phases of the cycle suggested that the immunised animals were unresponsive to endogenous oestradiol concentrations. Following ovariectomy, differences between immunised and control heifers were abolished, but the differences between treatment groups were restored following the insertion of a s.c. oestradiol implant, confirming our hypothesis. It would appear that the normal functioning of the oestradiol-mediated negative feedback control of gonadotrophin is dependent on the action of a follicular fluid protein. Having demonstrated the existence of such a follicular protein, it was of interest to examine the effects of the direct administration of follicular fluid proteins on gonadotrophin secretion and ovulation rate. Treatment failed to reduce peripheral FSH concentrations, although a significant dose-dependenthypersecretion of FSH was observed following the cessation of treatment. In addition, treatment with bovine or ovine follicular fluid proteins led to a significant increase in LH pulse amplitude. These effects on gonadotrophin secretion again occurred in the absence of any change in oestradiol concentrations, suggesting that the effects of treatment were most probably mediated via direct pituitary or hypothalamic effects. Collectively, we have demonstrated that follicular fluid proteins are important components in the control of the reproductive system of the cow. Such proteins are involved in the oestradiol-mediated negative feedback regulation of FSH and LH, the determination of LH pulse amplitude and the regulation of follicular growth. This latter role may represent the mechanism by which follicular dominance is effected and may present a useful target for future research attempting to develop techniques for the induction of twinning in cattle.

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14

Rekik, M. "Effect of rams and pretreatment with progesterone or melatonin upon gonadotrophin secretion, follicular development and reproductive performance of anoestrous adult ewes." Thesis, University of Reading, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383421.

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15

Kaplan, Hilton. "The control of prolactin secretion and the role of gonadotrophin releasing hormone in the production of concordant secretory spikes of luteinizing hormone and prolactin in the luteal phase of the menstrual cycle." Master's thesis, University of Cape Town, 1988. http://hdl.handle.net/11427/27203.

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The control of prolactin secretion is a complex interaction of peptides and neurotransmitters acting either in an inhibitory or stimulating way to effect final secretion of this hormone from the lactotrope cell in the anterior hypothalamus. These factors may act either directly on the lactotrope cell or indirectly by changing either dopamine restraint of prolactin secretion or by modulating peptide substances or neurotransmitters higher up in the hypothalamus. Gonadal steroids may also modulate the effect of peptides or dopamine at the level of the lactotrope. Prolactin's major role in the female rat is one of milk production post - partum, nurturing the young. It probably also has other physiological functions and may play a part in the menstrual cycle although this is controversial. Certainly, pulsatile secretion of prolactin during the menstrual cycle is well established and in the luteal phase this is concomitant with the secretion of luteinizing hormone. Theories explaining the synchronous surges seen during this phase of the menstrual cycle have been proposed and GnRH has been implicated in the genesis of the concordance of these secretory spikes. Using a potent GnRH antagonist an experiment was undertaken to establish the role of GnRH by blocking this hypothalamic peptide and observing the effect that this had on luteinizing hormone, prolactin and follicle stimulating hormone. In the first part of the thesis the control of prolactin secretion is reviewed. In the following section, an experiment was performed using a potent GnRH antagonist. A dose response curve was established for the antagonist action on LH. Then a twice maximum dose of this peptide was administered to three subjects in the midluteal phase of the menstrual cycle and the response of LH, prolactin and FSH was measured. The results indicate that although the GnRH antagonist significantly blocked LH secretory peaks, this action was not observed for either prolactin or FSH. This result is perhaps at variance with previous data which suggested that GnRH was responsible for concordant secretory spikes of LH and prolactin in the midluteal phase of the menstrual cycle.

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16

Wormald, Patricia J. "GnRH and neuropeptide regulation of gonadotropin secretion from cultured human pituitary cells." Doctoral thesis, University of Cape Town, 1988. http://hdl.handle.net/11427/27168.

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Gonadotropin-releasing hormone (GnRH) and its superactive analogues are currently being used in the treatment of a number of endocrine disorders, such as endometriosis, precocious puberty, infertility and prostatic cancer. Selection of these analogues for clinical use have been previously based on their activities in animal models. This thesis has therefore investigated the binding characteristics of the human GnRH receptor, in comparison to those of the rat receptor, as well as the activities of a number of GnRH analogues for stimulating luteinising hormone (LH) and follicle stimulating hormone (FSH) secretion from cultured human pituitary cells. The establishment of a human pituitary bioassay system has further made possible the investigation of the direct regulatory roles of GnRH and other neuropeptides in man. To date, such studies in man have been performed in vivo and are thus complicated by the simultaneous interactions of numerous modulators.

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17

Lo, Angelina Ching-Man. "Testosterone action on gonadotropin-II secretion from goldfish pituitary cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/mq22628.pdf.

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18

Willis, Heather June. "Noradrenergic and opioidergic regulation of gonadotropin secretion in the female pig." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq21652.pdf.

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19

Chandolia, Ramesh Kumar. "Early gonadotropin secretion and sexual maturation in bull calves and ram lambs." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq23981.pdf.

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20

Morton, Stephanie. "Effects of continuous treatment with gonadotropin-releasing hormone during the anovulatory season on gonadotropin secretion, follicular dynamics and ovulation in the mare." Texas A&M University, 2004. http://hdl.handle.net/1969.1/1551.

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Objectives were to determine if low-dose, continuous infusion of GnRH from Fall to Spring, would prevent seasonal anovulation in mares. Twenty Quarter Horse mares, ages 18 mo to 24 yrs, were stratified by age and body condition score and assigned randomly to either a saline control (n = 9) or GnRH (n = 11) treatment group. Treatments were instituted between September 23 and October 9, 2002. Gonadotropinreleasing hormone was delivered in 0.9% physiological saline via Alzet osmotic minipumps (Model 2004) placed sc at the base of the neck, with Silastic sham pumps placed in control mares. Pumps were inserted on day 3 following ovulation or during the follicular phase if ovulation had not occurred. Delivery rate of GnRH was 2.5 ug/h (60 ug/d) for the first 60 d, followed by 5.0 ug/h (120 ug/d) thereafter, with all pumps replaced every 30 d. By December 1, all mares had become anovulatory and remained anovulatory until February. Mean serum concentrations of LH were not affected by treatment in anovulatory mares. In contrast, control mares that exhibited ovulatory cycles after treatment onset had higher (P < 0.05) mean concentrations of LH during all phases of the estrous cycle except diestrus. Mean serum concentrations of FSH were not affected by treatment, but were lower (P < 0.05) from November though January relative to all other months in anovulatory mares. Interovulatory intervals in mares that cycled temporarily did not differ between groups. Ovulatory control mares had slightly larger (P < 0.10) follicles overall than GnRH-treated mares; however, ovulatory follicle diameters for control and GnRH-treated mares did not differ. Ovulatory control mares had higher (P < 0.10) mean concentrations of progesterone during metestrus and late diestrus. In a subgroup of control (n =5) and GnRH-treated (n = 5) mares, total releasable pools of LH in response to 1 mg GnRH did not differ between groups. Ovulation resumed in 3 control and 3 GnRH-treated mares by March 30. Results indicate that continuous infusion of native GnRH at the doses employed herein is not sufficient to maintain ovulatory cycles during the anovulatory season.

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21

RENARD, ALABEURTHE FRANCINE. "Pseudo-puberte precoce engendree par une secretion tumorale cerebrale de gonadotrophine chorionique : a propos d'un cas de teratome pluritissulaire." Reims, 1989. http://www.theses.fr/1989REIMM031.

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22

曾美好 and May-ho Tsang. "Dopaminergic regulation of gonadotropin-releasing hormone (GnRH) secretion and gene expression in a GnRH neuronal cell line." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1995. http://hub.hku.hk/bib/B31213698.

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23

Tsang, May-ho. "Dopaminergic regulation of gonadotropin-releasing hormone (GnRH) secretion and gene expression in a GnRH neuronal cell line /." Hong Kong : University of Hong Kong, 1995. http://sunzi.lib.hku.hk/hkuto/record.jsp?B17095219.

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24

Billiard, Julia. "New stimulators and a new mechanism of regulated secretion in pituitary gonadotropes /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/10544.

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25

Fletcher, Patrick Allen. "Modeling electrical spiking, bursting and calcium dynamics in gonadotropin releasing hormone (GnRH) secreting neurons." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/2574.

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The plasma membrane electrical activities of neurons that secrete gonadotropin releasing hormone (GnRH), referred to as GnRH neurons hereafter, have been studied extensively. A couple of mathematical models have been developed previously to explain different aspects of these activities including spontaneous spiking and responses to stimuli such as current injections, GnRH, thapsigargin (Tg) and apamin. The goal of this paper is to develop one single, minimal model that accounts for the experimental results reproduced by previously existing models and results that were not accounted for by these models. The latter includes two types of membrane potential bursting mechanisms and the associated calcium oscillations in the cytosol. One of them has not been reported in experimental literatures on GnRH neurons and is thus regarded as a model prediction. Other improvements achieved in this model include the incorporation of a more detailed description of calcium dynamics in a three dimensional cell body with the ion channels evenly distributed on the cell surface. Although the model is mainly based on data collected in cultured GnRH cell lines, we show that it is capable of explaining some properties of GnRH neurons observed in several of other preparations including mature GnRH neurons in hypothalamic slices. One potential explanation is suggested. A phenomenological reduction of this model into a simplified form is presented. The simplified model will facilitate the study of the roles of plasma membrane electrical activities on the pulsatile release of GnRH by these neurons when it is coupled with a model of pulsatile GnRH release based on the autoregulation mechanism.

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26

BERTHELIER-BIHOREAU, CLAIRE. "Analyse des mecanismes de couplage dans l'antehypophyse : fonctions de la cascade arachidonique et des flux calciques." Paris 6, 1988. http://www.theses.fr/1988PA066078.

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27

Bennani, Sanae. "Rôle des amines biogènes dans la régulation de la sécrétion de gonadotropine chez la truite arc-en-ciel : intéraction avec les stéroïdes sexuels." Rennes 1, 1995. http://www.theses.fr/1995REN10012.

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Dans le but d'approfondir l'etude des mecanismes impliques dans le controle steroidien du cycle reproducteur chez la truite arc-en-ciel femelle (oncorhynchus mykiss), nous nous sommes interesses au metabolisme des neuromediateurs aminergiques au niveau du cerveau anterieur et de l'hypophyse. Les taux de monoamines (dopamine, noradrenaline, serotonine) et de leurs principaux catabolites ont ete determines par chromatographie liquide haute performance couplee a une detection electrochimique, au niveau de diverses structures (telencephale, aire preoptique, hypothalamus, hypophyse) et dans differentes conditions physiologiques. Une etude realisee tout au long du cycle reproducteur a mis en evidence l'existence de variations importantes du metabolisme aminergique de l'axe hypothalamo-hypophysaire au cours de la periode periovulatoire, notamment une diminution du tumover dopaminergique avant ovulation, compatible avec les donnees de la litterature concernant une levee d'inhibition dopaminergique de la liberation de gonadotropine chez divers teleosteens. L'oestradiol et la testosterone exercent un retrocontrole positif sur la synthese de gonadotropine hypophysaire: ceci a pu etre mis en evidence au cours d'experimentations associant l'ovariectomie a un implant steroidien, chez la truite en debut ou en milieu de vitellogenese. Aucune variation des taux de gonadotropine circulante n'a pu par contre etre mise en evidence parallelement a l'augmentation des taux de peptide hypophysaire engendree par traitement par l'oestradiol: ceci suggere l'existence d'un retrocontrole inhibiteur de l'oestradiol sur la liberation de l'hormone. Une correlation positive est obtenue entre les taux d'oestradiol circulant et les taux de catecholamines hypothalamiques (dopamine et noradrenaline), suggerant que des voies catecholaminergiques pourraient etre impliquees dans le retrocontrole de l'oestradiol. Nous nous sommes interesses, dans un deuxieme temps, a l'etude des mecanismes de regulation de l'activite de la tyrosine hydroxylase (th) etape limitante de la synthese des catecholamines, ceci in vitro ou in vivo. Une inhibition competitive par la dopamine et une inhibition non competitive par les catecholoestrogenes ont ete mises en evidence, l'oestradiol s'averant inefficace. Nous avons egalement montre, chez des poissons immatures, en recrudescence ovarienne ou triploides, que les taux de catecholamines sont correles de facon differente a l'activite th in vitro et in vivo, selon la structure consideree: l'hypothalamus se caracterise ainsi par des taux eleves de neuromediateurs, associes a une faible activite th. L'etude du role de l'oestradiol dans la regulation de l'activite in vivo de l'enzyme a ete abordee chez des truites sexuellement immatures: une augmentation de l'activite th, suivie d'une augmentation des taux de catabolites dopaminergiques a ete observee dans le telencephale apres injection unique d'oestradiol ; aucune variation de l'activite enzymatique n'est pas contre decelee apres implant d'oestradiol (traitement de 3 semaines). Enfin, l'etude de la distribution neuroanatomique de la tyrosine hydroxylase a ete abordee par immunocytochimie, utilisant un anticorps monoclonal de souris. Les neurones th-immunoreactifs ont ete localises dans les bulbes olfractifs dans les parties ventrales et dorsales de l'aire ventrale du telencephale, dans les parois ventrales et laterales du recessus preoptique, dans le noyau preoptique parvocellulaire ainsi que dans divers noyaux hypothalamiques tels l'area pretectalis, le nucleus ventromedialis thalami, le nucleus posterioris periventricularis et le nucleus posterioris tuberi. Plusieurs neurones de type csf-contacting cell (cellules en contact avec le liquide cephalo-rachidien) ont ete localises dans le nucleus recessus lateralis et le nucleus saccus vasculosus

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28

Meikle, Ana. "Reproductive endocrinology of prepubertal and anestrous ewes : regulation of uterine sex steroid receptors by ovarian hormones and effects of estradiol on gonadotropin secretion and follicular growth /." Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 2001. http://epsilon.slu.se/avh/2001/91-576-5915-X.pdf.

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29

Jeanjean, Bruno. "Inhibition exercee par la substance p sur la secretion antehypophysaire de lh, induite par le gnrh, chez la ratte : role de l'estradiol-17beta et de la progesterone sur la secretion hypothalamique de la substance p, chez la guenon." Paris 6, 1988. http://www.theses.fr/1988PA066310.

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30

ARGYRIOU, ANASTASSIOS. "Etude de la steroidogenese dans le testicule de l'homme jeune." Paris 6, 1988. http://www.theses.fr/1988PA066026.

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31

Yvorra, Alain. "Croissance folliculaire et developpement du corps jaune chez le lezard vivipare, lacerta vivipara jacquin : evolution au cours du cycle sexuel et analyse des mecanismes de regulation." Paris 6, 1986. http://www.theses.fr/1986PA066270.

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32

CHEN, XING-LANG, and 陳杏亮. "Androgen-regulated gonadotropin secretion in anterior pituitary cells from male rats." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/46836776661628562770.

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33

Shiu, Li-Shiue, and 徐莉雪. "The response of pituitary hormones secretion after gonadotropin releasing hormone stimulation test in ovariohysterectomized rats." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/43679932081256498100.

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碩士<br>國立中興大學<br>獸醫學系暨研究所<br>100<br>Neutralization has been supposed as one of the main risk factors of obesity. Obesity associated with multiple endocrine alterations in the concentration of circulating hormones. Gonadotropin-releasing hormone (GnRH) is not only a primary regulator of gonadal function but also a possible factor which affects other pituitary cells out of gonadotrophs. Recently, it has been reported that GnRH might induce GH releasing, but the effects on GH and other pituitary hormones of GnRH still remained controversial. In this study, ovariohysterectomy (OHE) was performed in the 30 week-old female Sprague Dawley (SD) rats as experimental groups. Intact female rats were taken as control group. All rats were examined by GnRH stimulation test at different weeks (20, 24, and 30) after OHE. Evaluate the circulating GH, IGF-I, ACTH, TSH concentrations at different time points with commercial ELISA kits. In results, the levels of GH after stimulation in all OHE groups tended to lower than control but without significantly difference. In all groups, the levels of IGF-I at 30 min after stimulation were significantly lower than the basal IGF-I levels. The levels of ACTH at 30 min after GnRH administrating were significantly higher than the basal ACTH levels in all groups. Furthermore, both the basal and stimulated ACTH levels in all OHE groups were significantly higher than the control ones. The levels of TSH after stimulation in all OHE groups tended to higher than control ones but without significant difference. According to the results, GnRH administration could induce GH, ACTH and TSH secretion but reduce IGF-I levels in ovariohysterectomized rats. Whereas the effects of GnRH on GH secretion was not significant as we expected. The obesity after neutralization in female rats is probably accomplished with the elevation of IGF-I and ACTH.

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34

LI, XIAN-YANG, and 李顯揚. "Role of thyroid hormones in regulating the secretion of testosterone in response to gonadotropin and that of luteinizing hormone in response to gonadotropin-releasing hormone in male rats." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/56209860325763847608.

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Lin, Xiao-Qing, and 林曉清. "The studies of gonadotropins secretion and sex steroid hormones levels in athletic women and in men treated with clomphene citrate." Thesis, 1987. http://ndltd.ncl.edu.tw/handle/24899961412066104223.

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