Academic literature on the topic 'Granulosa and cumulus cells'

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Journal articles on the topic "Granulosa and cumulus cells"

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Feng, Wei Guo, and Zhi Fang Pan. "The Effect of Granulosa Cells Apoptosis on the Cumulus Expansion and the Developmental Competence of Bovine Oocytes." Advanced Materials Research 997 (August 2014): 251–54. http://dx.doi.org/10.4028/www.scientific.net/amr.997.251.

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In this study, we used the well in the well (WIW) culture system to study the effect of granulosa cells apoptosis on the cumulus expansion and the developmental competence of bovine oocytes. Results: The apoptosis of granulosa cells affect the cumulus expansion of bovine oocytes significantly. Especially when the percentage of granulosa cells apoptosis exceed 40%, the cumulus expansion was worse. The cumulus expansion affect the oocyte developmental competence of bovine oocytes significantly. The developmental competence of bovine oocyte increases with the increasing of cumulus expansion.
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Pogrmic-Majkic, Kristina, Gordana Kosanin, Dragana Samardzija Nenadov та ін. "Rosiglitazone increases expression of steroidogenic acute regulatory protein and progesterone production through PPARγ–EGFR–ERK1/2 in human cumulus granulosa cells". Reproduction, Fertility and Development 31, № 11 (2019): 1647. http://dx.doi.org/10.1071/rd19108.

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The mechanism by which rosiglitazone (ROSI: a thiazolidinedione (TZD)) affects steroid production in undifferentiated human granulosa cells is not known. In this study, cultured human cumulus granulosa cells were exposed to ROSI and pharmacological inhibitors of the extracellular signal-regulated kinase 1/2 (ERK1/2), epidermal growth factor receptor (EGFR) and peroxisome proliferator-activated receptor gamma (PPARγ) signalling pathways. Expression of progesterone biosynthetic enzymes, PPARγ and PPARα, progesterone production and ERK1/2 activation were analysed. After 48h, 30μM ROSI increased S
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Salustri, Antonietta, Antonella Camaioni, and Cristina D'Alessandris. "Endocrine and paracrine regulation of cumulus expansion." Zygote 4, no. 04 (1996): 313–15. http://dx.doi.org/10.1017/s0967199400003312.

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In a Graafian follicle, granulosa cells are classified into two principal cell subpopulations: cumulus cells, which are closely associated with the oocyte to form the cumulus cell-oocyte complex (COC), and mural granulosa cells, which are organised as a stratified epithelium at the periphery of the follicle. Following the preovulatory gonadotropin surge, cumulus cells lose contact with mural granulosa cells and start to synthesise and secrete a large amount of hyaluronan (HA), a glycosaminoglycan with high molecular weight and large hydrodynamic domains (Salustriet al., 1992). Proteins derived
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Ünal, Murat Serkant, and Cihan Kabukçu. "Isolation of Human Cumulus Granulosa Cells." Van Medical Journal 29, no. 1 (2022): 84–89. http://dx.doi.org/10.5505/vtd.2022.20805.

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Bryja, Artur, Wojciech Pieńkowski, Katarzyna Stefańska, et al. "Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture." Medical Journal of Cell Biology 8, no. 4 (2020): 152–57. http://dx.doi.org/10.2478/acb-2020-0019.

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Abstract The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture. These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells. Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone. Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis w
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Chaffin, Charles L., Young S. Lee, Catherine A. VandeVoort, Bela G. Patel, and Keith E. Latham. "Rhesus Monkey Cumulus Cells Revert to a Mural Granulosa Cell State After an Ovulatory Stimulus." Endocrinology 153, no. 11 (2012): 5535–45. http://dx.doi.org/10.1210/en.2012-1733.

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Abstract Follicular somatic cells (mural granulosa cells and cumulus cells) and the oocyte communicate through paracrine interactions and through direct gap junctions between oocyte and cumulus cells. Considering that mural and cumulus cells arise through a common developmental pathway and that their differentiation is essential to reproductive success, understanding how these cells differ is a key aspect to understanding their critical functions. Changes in global gene expression before and after an ovulatory stimulus were compared between cumulus and mural granulosa cells to test the hypothe
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Watson, Laura N., David G. Mottershead, Kylie R. Dunning, Rebecca L. Robker, Robert B. Gilchrist, and Darryl L. Russell. "Heparan Sulfate Proteoglycans Regulate Responses to Oocyte Paracrine Signals in Ovarian Follicle Morphogenesis." Endocrinology 153, no. 9 (2012): 4544–55. http://dx.doi.org/10.1210/en.2012-1181.

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In the ovarian follicle, oocyte-secreted factors induce cumulus-specific genes and repress mural granulosa cell specific genes to establish these functionally distinct cell lineages. The mechanism establishing this precise morphogenic pattern of oocyte signaling within the follicle is unknown. The present study investigated a role for heparan sulphate proteoglycans (HSPG) as coreceptors mediating oocyte secreted factor signaling. In vitro maturation of cumulus oocyte complexes in the presence of exogenous heparin, which antagonizes HSPG signaling, prevented cumulus expansion and blocked the in
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Pacella-Ince, Leanne, Deirdre L. Zander-Fox, and Michelle Lane. "Mitochondrial SIRT5 is present in follicular cells and is altered by reduced ovarian reserve and advanced maternal age." Reproduction, Fertility and Development 26, no. 8 (2014): 1072. http://dx.doi.org/10.1071/rd13178.

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Women with reduced ovarian reserve or advanced maternal age have an altered metabolic follicular microenvironment. As sirtuin 5 (SIRT5) senses cellular metabolic state and post-translationally alters protein function, its activity may directly impact on oocyte viability and pregnancy outcome. Therefore, we investigated the role of SIRT5 in relation to ovarian reserve and maternal age. Women (n = 47) undergoing routine IVF treatment were recruited and allocated to one of three cohorts based on ovarian reserve and maternal age. Surplus follicular fluid, granulosa and cumulus cells were collected
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Alyoshina, N. M., V. R. Rousanova, L. A. Malchenko, et al. "Analysis of the Ovarian Marker Genes Expression Revealed the Antagonistic Effects of Serotonin and Androstenedione on the Functional State of Mouse Granulosa Cells in Primary Culture." Онтогенез 54, no. 3 (2023): 183–95. http://dx.doi.org/10.31857/s0475145023030023.

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Primary culture of granulosa cells is a prerequisite for a complete study of the normal functioning of the ovary and its pathologies. In this work, we selected the optimal protocol for obtaining a primary culture of mouse granulosa cells in the most functionally active state and revealed the effects of androstenedione and serotonin on the expression of ovarian markers that reflect the functional status of granulosa cells. The morpho-functional analysis of the ovary after PMSG stimulation revealed that 48 hours after PMSG stimulation is the optimal time for obtaining granulosa cells in the most
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Hosoe, M., K. Kaneyama, K. Ushizawa, S. Akagi, J. Noguchi, and T. Takahashi. "233 TEMPOROSPATIAL EXPRESSION PROFILES OF GROWTH AND DIFFERENTIATION FACTOR-9 AND BONE MORPHOGENETIC PROTEIN-15 GENES IN THE BOVINE OVARY." Reproduction, Fertility and Development 18, no. 2 (2006): 225. http://dx.doi.org/10.1071/rdv18n2ab233.

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Bone morphogenetic protein (BMP) family proteins regulate the folliculogenesis and ovulation rate in mammals. Of the members of BMP family, growth and differentiation factor-9 (GDF-9) and BMP-15 are oocyte-derived proteins that play critical roles in follicular development. In the present study, we characterized the temporospatial expression patterns of GDF-9 and BMP-15 in bovine ovaries. Bovine ovaries were obtained from local abattoir. Cumulus–oocyte complexes (COCs) and mural granulosa cells were collected by aspiration of follicles 2–5 mm in diameter. Follicular aspiration was done the day
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Dissertations / Theses on the topic "Granulosa and cumulus cells"

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De, Cesaro Matheus Pedrotti. "Regulação do sistema peptídeos natriuréticos nas células da granulosa e do cumulus na foliculogênese em bovinos." Universidade Federal de Santa Maria, 2017. http://repositorio.ufsm.br/handle/1/11736.

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This thesis aims to contribute to the knowledge of the regulation and function of the natriuretic peptide (NP) system during follicular dominance, ovulation, oocyte meiosis resumption and cumulus cells expansion in cattle. In the first study, the NP system was characterized in bovine COC. Moreover, NPPA and NPPC increased cGMP levels in cumulus cells and oocyte after 3 hours of culture, preventing the increase of cAMP in oocyte in the presence of forskolin. In the second study, using in vivo experimental models, none of the three NPs were detected in the granulosa cells during follicular
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Pastorello, Marilia. "HORMONE RECEPTORS AND STEROIDOGENIC ENZYMES IN OOCYTES, CUMULUS AND GRANULOSA CELLS, FOLLICULAR FLUID HORMONE MILIEU, AND FOLLICLE BLOOD PERFUSION IN MARES." OpenSIUC, 2021. https://opensiuc.lib.siu.edu/theses/2810.

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Ovulatory follicle development and associated oocyte maturation involve complex coordinated molecular and cellular mechanisms not fully understood. This study addresses, for the first time in any species, the relationships among diameter, vascularization, follicular-fluid factors, and gene expression for follicle growth, steroidogenesis, angiogenesis and apoptosis in granulosa/cumulus cells and oocytes during different stages from the beginning of largest/ovulatory follicle to impending ovulation. Follicle dynamics was tracked and follicle-wall blood flow evaluated daily through transrectal ul
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Semplici, Bianca. "Bitter taste receptors: new players in female and male fertility." Doctoral thesis, Università di Siena, 2021. http://hdl.handle.net/11365/1148668.

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The mammalian taste perception provides valuable information about the nature and quality of food. Taste transduction involves the interaction of molecules (i.e. tastants) with taste receptor (TASRs) whose expression isn’t limited to the oral cavity; indeed, many studies showed how these receptors can be found also in extraoral location, wherein their function appears less obvious and remains to be understood in most cases. The expression of these receptors outspreads also to brain, respiratory tract, urinary bladder and testis, where some of these receptors are expressed in different st
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Joharatnam, Jalini. "Insulin signalling in granulosa cells." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9784.

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Polycystic ovary syndrome (PCOS) is characterised by hyperandrogenism and insulin resistance. Granulosa cells in PCOS demonstrate impaired insulin-induced glucose uptake and lactate accumulation, suggesting a post receptor, signalling pathway-specific impairment of insulin action. Gonadotrophins are also important in the regulation of glucose metabolism by of granulosa cells. The first objective of this project was to use KK1 cells an immortalised mouse granulosa cell line, to characterise insulin, androgen and FSH signalling as well as glucose metabolism. Cell lysates were subjected to wester
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Paton, Anne Claire. "Apoptosis of human granulosa-lutein cells." Thesis, King's College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243501.

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Assidi, Mourad. "Oocyte competence and cumulus cells gene expression." Thesis, Université Laval, 2010. http://www.theses.ulaval.ca/2010/27679/27679.pdf.

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Quinn, Michael Corwin James, and n/a. "Gene expression profiling of human granulosa cells." University of Otago. Department of Anatomy & Structural Biology, 2005. http://adt.otago.ac.nz./public/adt-NZDU20070501.144002.

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Human granulosa cells play an important role in the follicle, providing the oocyte with nutrients and growth factors to ensure successful ovulation. Normal granulosa cell functioning is thus crucial to human fertility. By studying their transcriptome, the mechanisms underpinning follicle development and infertility will be better understood. In this study, granulosa cells were retrieved at the time of oocyte removal for in vitro fertilization (IVF). Cells were purified through a combination of a Percoll gradient to remove red blood cells and positive selection of granulosa cell aggregations.
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Bains, Harvinder. "The oestrogen receptor in porcine granulosa cells." Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288793.

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Kuran, Mehmet. "Studies on bovine granulosa cells in culture." Thesis, University of Aberdeen, 1995. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU543200.

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This study includes investigations into bovine granulosa cell differentiation, the response of these cells to gonadotrophin (follicle-stimulating hormone (FSH), luteinizing hormone (LH) and pregnant mares' serum gonadotrophin (PMSG)) stimulation, and the neutralizing capacity of a monoclonal antibody to PMSG (APMSG) on the response of gonadotrophin-stimulated bovine granulosa cells using a serum-free culture system. The parameters measured were cell number (mug DNA), cytoplasmic:nuclear ratio (mug protein/mug DNA), cell size (mum diameter) and cell steroidogenesis (progesterone, ng/mug DNA; oe
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Mattice, David Frederick. "Gonadotropic regulation of steroidogenesis in rat granulosa cells." Thesis, University of Ottawa (Canada), 1987. http://hdl.handle.net/10393/5220.

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Books on the topic "Granulosa and cumulus cells"

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Yan, Zhaoping. Expression and regulation of vascular endothelial growth factor in human granulosa cells in vitro. [s.n.], 1994.

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A, Gewirtz David, Holt Shawn Edan, and Grant Steven, eds. Apoptosis and senescence in cancer chemotherapy and radiotherapy. 2nd ed. Humana, 2006.

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A, Gewirtz David, Holt Shawn E, and Grant Steven, eds. Apoptosis and senescence in cancer chemotherapy and radiotherapy. Humana Press, 2007.

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Wyne, Kathleen L. Regulation of apolipoprotein E synthesis in rat ovarian granulosa cells. 1989.

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Siemann, Dietmar W. Tumor Microenvironment. Wiley & Sons, Incorporated, John, 2011.

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Siemann, Dietmar W. Tumor Microenvironment. Wiley & Sons, Incorporated, John, 2011.

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Siemann, Dietmar W. Tumor Microenvironment. Wiley & Sons, Incorporated, John, 2010.

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Tumor microenvironment. Wiley, 2011.

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X. Yuan, J. Wang*, and L. Li. GATA4 overexpression protects goose granulosa cells from the cytotoxic effects of 25-hydroxycholesterol. Verlag Eugen Ulmer, 2019. http://dx.doi.org/10.1399/eps.2019.282.

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Maier, Richard M. Effect of blood monocytes and lymphocytes on progesterone secretion by porcine granulosa cells IN VITRO. 1988.

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Book chapters on the topic "Granulosa and cumulus cells"

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Mehlmann, Lisa M. "Signaling for Meiotic Resumption in Granulosa Cells, Cumulus Cells, and Oocyte." In Oogenesis. Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-826-3_12.

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Fragouli, Elpida, and Dagan Wells. "Transcriptomic Analysis of Cumulus and Granulosa Cells as a Marker of Embryo Viability." In Human Gametes and Preimplantation Embryos. Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-6651-2_17.

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Mao, Jian, and Lin Liu. "Generation of iPS Cells from Granulosa Cells." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/7651_2014_135.

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Faustini, Massimo, Giulio Curone, Maria L. Torre, and Daniele Vigo. "Bioencapsulation of Oocytes and Granulosa Cells." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8600-2_9.

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Faustini, Massimo, Stella Agradi, Daniele Vigo, Maria L. Torre, and Giulio Curone. "Bioencapsulation of Oocytes and Granulosa Cells." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3609-1_10.

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Mao, Jian, and Lin Liu. "Erratum to: Generation of iPS Cells from Granulosa Cells." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/7651_2015_308.

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Fitz, T. A., M. M. Marr, T. L. Walden, W. A. Schmidt, and C. A. Winkel. "Gap Junctions in Transformed Rat Granulosa Cells." In Growth Factors and the Ovary. Springer US, 1989. http://dx.doi.org/10.1007/978-1-4684-5688-2_27.

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Chen, T. T., K. Gao, M. A. Shelton, and D. D. Hatmaker. "Coordinated Receptor Regulation in Ovarian Granulosa Cells." In Advances in Experimental Medicine and Biology. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4684-5395-9_46.

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Artini, Paolo Giovanni. "Gene Expression in Cumulus Cells and Oocyte Quality." In ISGE Series. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-23865-4_5.

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Richards, JoAnne S., Richard C. Kurten, Susan L. Fitzpatrick, Ria B. Oonk, and Winona L. Wong. "Diverse Mechanisms Regulating Gene Expression in Granulosa Cells." In Follicle Stimulating Hormone. Springer New York, 1992. http://dx.doi.org/10.1007/978-1-4684-7103-8_11.

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Conference papers on the topic "Granulosa and cumulus cells"

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Marynowicz, Weronika, Paulina Glód, Justyna Gogola-Mruk, Dawid Maduzia, and Anna Ptak. "703 Changes in the energy metabolism of the cumulus granulosa cells in patients with premature ovarian insufficiency." In ESGO 2024 Congress Abstracts. BMJ Publishing Group Ltd, 2024. http://dx.doi.org/10.1136/ijgc-2024-esgo.470.

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Nur Kasman, AA Muhammad, Budi Santoso, Widjiati Widjiati, et al. "The Viability Differences on Oocyte Vitrification Surrounded by Cumulus Cells and Oocyte Vitrification not Surrounded by Cumulus Cells after In Vitro Maturation." In ICBBE '20: 2020 7th International Conference on Biomedical and Bioinformatics Engineering. ACM, 2020. http://dx.doi.org/10.1145/3444884.3444912.

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Cai, Xiaohui, Huan Shen, Qun Lu, Hongjing Han, Cheng Shi, and Yanbing Wang. "The Effect of Mono-(2-Ethylhexyl) Phthalate on Human Granulosa Cells." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5516194.

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Zeringue, H. C., D. J. Beebe, and M. B. Wheeler. "Removal of Cumulus Cells from Mammilian Oocytes in a Microfluidic System." In 2000 Solid-State, Actuators, and Microsystems Workshop. Transducer Research Foundation, Inc., 2000. http://dx.doi.org/10.31438/trf.hh2000.10.

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Gupta, Chanchal, Yash Chhabra, Trimbak Chapekar, Subrata Sinha, and Kalpana Luthra. "Abstract A37: Differential response of LH and hCG on ovarian granulosa cells." In Abstracts: AACR International Conference on Frontiers in Cancer Prevention Research‐‐ Dec 6–9, 2009; Houston, TX. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1940-6207.prev-09-a37.

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Savita, Pannu, and Khullar Harsha. "Two interesting cases of granulosa cell tumor: A case report." In 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685326.

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Introduction: Granulosa cell tumor (GCT) is an ovarian malignancy that arise from granulosa cells of the ovary. This tumour is a type of the sex cord-gonadal stromal tumour. GCT have good prognosis in comparison with other epithelial tumors. Methodology: Two cases of granulosa cell tumors were diagnosed in sir Ganga ram hospital, Rajendernagar, New Delhi in December 2015 and January 2016. The patient’s age, clinical manifestations, radiological and histopathological findings were evaluated. One was in perimenopausal age group and other case was in postmenopausal age group. The clinical manifes
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Savita, Pannu, and Khullar Harsha. "Two interesting cases of granulosa cell tumor: A case report." In 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685309.

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Introduction: Granulosa cell tumor (GCT) is an ovarian malignancy that arise from granulosa cells of the ovary. This tumour is a type of the sex cord-gonadal stromal tumour. GCT have good prognosis in comparison with other epithelial tumors. Methodology: Two cases of granulosa cell tumors were diagnosed in sir Ganga ram hospital, Rajender Nagar, New Delhi in December 2015 and January 2016. The patient’s age, clinical manifestations, radiological and histopathological findings were evaluated. One was in perimenopausal age group and other case was in postmenopausal age group. The clinical manife
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Paolanti, Marina, Marco Mameli, Emanuele Frontoni, et al. "Automatic Classification of Human Granulosa Cells in Assisted Reproductive Technology using vibrational spectroscopy imaging." In 2020 25th International Conference on Pattern Recognition (ICPR). IEEE, 2021. http://dx.doi.org/10.1109/icpr48806.2021.9412544.

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Liu, Ying, Vasu Punj, Hao Hong, Elena Enbom, Robert Maxson, and Louis Dubeau. "Abstract 2395: Effect of Brca1 knockout on gene expression in mouse ovarian granulosa cells." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-2395.

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Lv, Xiangmin, Chunbo He, Guohua Hua, Jixin Dong, John S. Davis, and Cheng Wang. "Abstract A37: Yes-associated protein 1 (YAP) in the growth and tumorigenesis of ovarian granulosa cells." In Abstracts: AACR Special Conference: Advances in Ovarian Cancer Research: Exploiting Vulnerabilities; October 17-20, 2015; Orlando, FL. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1557-3265.ovca15-a37.

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Reports on the topic "Granulosa and cumulus cells"

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Meidan, Rina, and Robert Milvae. Regulation of Bovine Corpus Luteum Function. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604935.bard.

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The main goal of this research plan was to elucidate regulatory mechanisms controlling the development, function of the bovine corpus luteum (CL). The CL contains two different sterodigenic cell types and therefore it was necessary to obtain pure cell population. A system was developed in which granulosa and theca interna cells, isolated from a preovulatory follicle, acquired characteristics typical of large (LL) and small (SL) luteal cells, respectively, as judged by several biochemical and morphological criteria. Experiments were conducted to determine the effects of granulosa cells removal
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Wolfenson, David, William W. Thatcher, Rina Meidan, Charles R. Staples, and Israel Flamenbaum. Hormonal and Nutritional Stretegies to Optimize Reproductive Function and Improve Fertility of Dairy Cattle during Heat Stress in Summer. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568773.bard.

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The BARD program includes two main parts. In the first, experiments were conducted to complete our understanding of the mechanisms responsible for the impairment of reproductive functions under heat stress. Experiments focused on follicular development and function, since results obtained in our previous BARD project indicate that the preovulatory follicle is susceptible to heat stress. The theca cells, sensitive to thermal stress, produced less androgen during the summer, as well as during the autumn. Similarly, luteinized theca cells obtained from cows in summer produced much less progestero
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Nikolov, Gueorgui, Georgi N. Georgiev, Elena Marinova, Milena Mourdjeva, and Rossitza Konakchieva. Up-regulation of MT1 and MT2 Receptors by In Vitro Melatonin and Modulation of Alpha-tubulin and Aromatase P450 Expression in Human Granulosa-lutein Cells. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, 2020. http://dx.doi.org/10.7546/crabs.2020.03.07.

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Techakumphu, Mongkol, Pranee Numchaisrika, Ratajuk Rungsiwiwat, Somchai Suwajanakorn, Kamtorn Pruksananonda, and Pramuan Virutamasen. Development of somatic cell nuclear transfer techique for biomedical and agricultural research : report. Chulalongkorn University, 2004. https://doi.org/10.58837/chula.res.2004.85.

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To develop somatic cell nuclear transfer by using the rabbit as a mode in biomedical and agricultural research. The experiments are described in nine chapters: Chapter 1: Introduction, Chapter 2: Superovulation and oocyte recipient production in rabbits, Chapter 3: A technique for adult and foetal fibroblast cell culture, for donor cell preparation in somatic nuclear transfer, Chapter 4: Preliminary studies on somatic cell nuclear transfer in rabbits, Chapter 5: In vitro development of rabbit cloned embryos using adult fibroblast cells, after post-activation treatment with cyelohexamide and 6-
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Wolfenson, David, William W. Thatcher, and James E. Kinder. Regulation of LH Secretion in the Periovulatory Period as a Strategy to Enhance Ovarian Function and Fertility in Dairy and Beef Cows. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586458.bard.

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The general research objective was to increase herd pregnancy rates by enhancing corpus luteum (CL) function and optimizing follicle development, in order to increase conception rate and embryo survival. The specific objectives were: to determine the effect of the duration of the preovulatory LH surge on CL function; to determine the function of LH during the postovulatory period on CL development; to optimize CL differentiation and follicle development by means of a biodegradable GnRH implant; to test whether optimization of CL development and follicle dynamics in timed- insemination protocol
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