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Journal articles on the topic 'Granulosa and cumulus cells'

Author: Grafiati

Published: 17 June 2021

Last updated: 29 July 2025

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1

Feng, Wei Guo, and Zhi Fang Pan. "The Effect of Granulosa Cells Apoptosis on the Cumulus Expansion and the Developmental Competence of Bovine Oocytes." Advanced Materials Research 997 (August 2014): 251–54. http://dx.doi.org/10.4028/www.scientific.net/amr.997.251.

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In this study, we used the well in the well (WIW) culture system to study the effect of granulosa cells apoptosis on the cumulus expansion and the developmental competence of bovine oocytes. Results: The apoptosis of granulosa cells affect the cumulus expansion of bovine oocytes significantly. Especially when the percentage of granulosa cells apoptosis exceed 40%, the cumulus expansion was worse. The cumulus expansion affect the oocyte developmental competence of bovine oocytes significantly. The developmental competence of bovine oocyte increases with the increasing of cumulus expansion.

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Pogrmic-Majkic, Kristina, Gordana Kosanin, Dragana Samardzija Nenadov та ін. "Rosiglitazone increases expression of steroidogenic acute regulatory protein and progesterone production through PPARγ–EGFR–ERK1/2 in human cumulus granulosa cells". Reproduction, Fertility and Development 31, № 11 (2019): 1647. http://dx.doi.org/10.1071/rd19108.

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The mechanism by which rosiglitazone (ROSI: a thiazolidinedione (TZD)) affects steroid production in undifferentiated human granulosa cells is not known. In this study, cultured human cumulus granulosa cells were exposed to ROSI and pharmacological inhibitors of the extracellular signal-regulated kinase 1/2 (ERK1/2), epidermal growth factor receptor (EGFR) and peroxisome proliferator-activated receptor gamma (PPARγ) signalling pathways. Expression of progesterone biosynthetic enzymes, PPARγ and PPARα, progesterone production and ERK1/2 activation were analysed. After 48h, 30μM ROSI increased S

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3

Salustri, Antonietta, Antonella Camaioni, and Cristina D'Alessandris. "Endocrine and paracrine regulation of cumulus expansion." Zygote 4, no. 04 (1996): 313–15. http://dx.doi.org/10.1017/s0967199400003312.

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In a Graafian follicle, granulosa cells are classified into two principal cell subpopulations: cumulus cells, which are closely associated with the oocyte to form the cumulus cell-oocyte complex (COC), and mural granulosa cells, which are organised as a stratified epithelium at the periphery of the follicle. Following the preovulatory gonadotropin surge, cumulus cells lose contact with mural granulosa cells and start to synthesise and secrete a large amount of hyaluronan (HA), a glycosaminoglycan with high molecular weight and large hydrodynamic domains (Salustriet al., 1992). Proteins derived

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4

Ünal, Murat Serkant, and Cihan Kabukçu. "Isolation of Human Cumulus Granulosa Cells." Van Medical Journal 29, no. 1 (2022): 84–89. http://dx.doi.org/10.5505/vtd.2022.20805.

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Bryja, Artur, Wojciech Pieńkowski, Katarzyna Stefańska, et al. "Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture." Medical Journal of Cell Biology 8, no. 4 (2020): 152–57. http://dx.doi.org/10.2478/acb-2020-0019.

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Abstract The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture. These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells. Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone. Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis w

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Chaffin, Charles L., Young S. Lee, Catherine A. VandeVoort, Bela G. Patel, and Keith E. Latham. "Rhesus Monkey Cumulus Cells Revert to a Mural Granulosa Cell State After an Ovulatory Stimulus." Endocrinology 153, no. 11 (2012): 5535–45. http://dx.doi.org/10.1210/en.2012-1733.

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Abstract Follicular somatic cells (mural granulosa cells and cumulus cells) and the oocyte communicate through paracrine interactions and through direct gap junctions between oocyte and cumulus cells. Considering that mural and cumulus cells arise through a common developmental pathway and that their differentiation is essential to reproductive success, understanding how these cells differ is a key aspect to understanding their critical functions. Changes in global gene expression before and after an ovulatory stimulus were compared between cumulus and mural granulosa cells to test the hypothe

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Watson, Laura N., David G. Mottershead, Kylie R. Dunning, Rebecca L. Robker, Robert B. Gilchrist, and Darryl L. Russell. "Heparan Sulfate Proteoglycans Regulate Responses to Oocyte Paracrine Signals in Ovarian Follicle Morphogenesis." Endocrinology 153, no. 9 (2012): 4544–55. http://dx.doi.org/10.1210/en.2012-1181.

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In the ovarian follicle, oocyte-secreted factors induce cumulus-specific genes and repress mural granulosa cell specific genes to establish these functionally distinct cell lineages. The mechanism establishing this precise morphogenic pattern of oocyte signaling within the follicle is unknown. The present study investigated a role for heparan sulphate proteoglycans (HSPG) as coreceptors mediating oocyte secreted factor signaling. In vitro maturation of cumulus oocyte complexes in the presence of exogenous heparin, which antagonizes HSPG signaling, prevented cumulus expansion and blocked the in

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Pacella-Ince, Leanne, Deirdre L. Zander-Fox, and Michelle Lane. "Mitochondrial SIRT5 is present in follicular cells and is altered by reduced ovarian reserve and advanced maternal age." Reproduction, Fertility and Development 26, no. 8 (2014): 1072. http://dx.doi.org/10.1071/rd13178.

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Women with reduced ovarian reserve or advanced maternal age have an altered metabolic follicular microenvironment. As sirtuin 5 (SIRT5) senses cellular metabolic state and post-translationally alters protein function, its activity may directly impact on oocyte viability and pregnancy outcome. Therefore, we investigated the role of SIRT5 in relation to ovarian reserve and maternal age. Women (n = 47) undergoing routine IVF treatment were recruited and allocated to one of three cohorts based on ovarian reserve and maternal age. Surplus follicular fluid, granulosa and cumulus cells were collected

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9

Alyoshina, N. M., V. R. Rousanova, L. A. Malchenko, et al. "Analysis of the Ovarian Marker Genes Expression Revealed the Antagonistic Effects of Serotonin and Androstenedione on the Functional State of Mouse Granulosa Cells in Primary Culture." Онтогенез 54, no. 3 (2023): 183–95. http://dx.doi.org/10.31857/s0475145023030023.

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Primary culture of granulosa cells is a prerequisite for a complete study of the normal functioning of the ovary and its pathologies. In this work, we selected the optimal protocol for obtaining a primary culture of mouse granulosa cells in the most functionally active state and revealed the effects of androstenedione and serotonin on the expression of ovarian markers that reflect the functional status of granulosa cells. The morpho-functional analysis of the ovary after PMSG stimulation revealed that 48 hours after PMSG stimulation is the optimal time for obtaining granulosa cells in the most

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10

Hosoe, M., K. Kaneyama, K. Ushizawa, S. Akagi, J. Noguchi, and T. Takahashi. "233 TEMPOROSPATIAL EXPRESSION PROFILES OF GROWTH AND DIFFERENTIATION FACTOR-9 AND BONE MORPHOGENETIC PROTEIN-15 GENES IN THE BOVINE OVARY." Reproduction, Fertility and Development 18, no. 2 (2006): 225. http://dx.doi.org/10.1071/rdv18n2ab233.

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Bone morphogenetic protein (BMP) family proteins regulate the folliculogenesis and ovulation rate in mammals. Of the members of BMP family, growth and differentiation factor-9 (GDF-9) and BMP-15 are oocyte-derived proteins that play critical roles in follicular development. In the present study, we characterized the temporospatial expression patterns of GDF-9 and BMP-15 in bovine ovaries. Bovine ovaries were obtained from local abattoir. Cumulus–oocyte complexes (COCs) and mural granulosa cells were collected by aspiration of follicles 2–5 mm in diameter. Follicular aspiration was done the day

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Hosoe, M., K. Ushizawa, K. G. Hayashi, and T. Takahashi. "187 DIFFERENTIAL EXPRESSIONS OF GROWTH AND DIFFERENTIATION FACTOR 9 AND BONE MORPHOGENETIC PROTEIN 15 GENES IN OVARIES OF THE CALF AND COW." Reproduction, Fertility and Development 23, no. 1 (2011): 195. http://dx.doi.org/10.1071/rdv23n1ab187.

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It has been reported that prepubertal calf oocytes are less developmentally competent than those obtained from cows. The bone morphogenetic protein (BMP) family of proteins regulate folliculogenesis and the ovulation rate in mammals. Of the members of the BMP family, growth and differentiation factor 9 (GDF9) and BMP15 are oocyte-derived proteins that play critical roles in granulosa cell proliferation and differentiation. In the present study, we characterised the gene expression of bovine GDF9 and BMP15 in calf and adult cow ovaries. The ovaries obtained from 4 calves at 9 to 11 months old a

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12

Shen, Xiangju, Takashi Miyano, and Seishiro Kato. "Promotion of follicular antrum formation by pig oocytes in vitro." Zygote 6, no. 1 (1998): 47–54. http://dx.doi.org/10.1017/s0967199400005074.

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SummaryPig oocyte–cumulus–granulosa cell complexes (OCG complexes) from pig early antral follicles reorganise an antrum under the stimulation of FSH. The purpose of this study was to examine the role of the oocytes in antrum formation. In the first experiment, oocyte–cumulus complexes were removed from pig OCG complexes, and the antrum formation of parietal granulosa cells themselves (PGs) was examined. Antrum formation by sham-operated OCG complexes (OC/G complexes), in which the connections between the oocyte–cumulus complexes and the parietal granulosa cells had been disrupted, was also exa

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Marques, A., P. Santos, G. Antunes, A. Chaveiro та F. Moreira da Silva. "Effect of α-tocopherol on in vitro maturation of bovine cumulus-oocyte complexes". Canadian Journal of Animal Science 88, № 3 (2008): 463–67. http://dx.doi.org/10.4141/cjas07139.

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This study determined: the effects of α-tocopherol on apoptotic and necrotic levels of cumulus cells after in vitro maturation of bovine oocytes; whether exposure to α-tocopherol facilitates the development of bovine enclosed oocytes to metaphase II; and the effects of this antioxidant on apoptotic and necrotic levels of granulosa cells cultured in vitro. In conclusion, supplementation with α-tocopherol on in vitro maturation of bovine oocytes has a detrimental effect on the ability of oocytes to reach metaphase II, increasing the number of apoptotic and necrotic cumulus cells of bovine cumulu

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14

Cotterill, Matthew, Sally L. Catt, and Helen M. Picton. "Characterisation of the cellular and molecular responses of ovine oocytes and their supporting somatic cells to pre-ovulatory levels of LH and FSH during in vitro maturation." REPRODUCTION 144, no. 2 (2012): 195–207. http://dx.doi.org/10.1530/rep-12-0031.

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The response of Graafian follicles to pre-ovulatory surge levels of FSH and LH in vivo triggers the terminal differentiation of granulosa cells and oocyte maturation. In polyovular species, the LH-driven signalling uses the epidermal growth factor (EGF)-like ligands AREG, EREG and BTC to promote oocyte maturation and cumulus expansion. This experimental series used a physiologically relevant ovine in vitro maturation (IVM) system to evaluate the impact of exposure to pre-ovulatory levels (100 ng/ml) of LH and FSH on ovine cumulus cell expression of EGF-like ligands in vitro. The serum-free she

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15

Mattioli, M., B. Barboni, and L. Gioia. "Activation of protein kinase A and protein kinase C mediates the depolarising effect of LH in ovine cumulus-corona cells." Journal of Endocrinology 150, no. 3 (1996): 445–56. http://dx.doi.org/10.1677/joe.0.1500445.

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Abstract We have studied the changes in membrane potential induced by LH in cumulus and granulosa cells isolated from sheep antral follicles. The investigation was carried out by using a non-invasive technique based on the use of a membrane potential sensitive probe, bis-oxonol. The membrane potential of mural granulosa cells was totally unaffected by LH, while that of cumulus or corona cells showed a marked depolarisation, starting 2–3 min after the addition of the hormone and plateauing after 5–10 min. None of the cells tested reacted to FSH. In the second part of the experiment the role of

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16

Esencan, Ecem, Mauro Cozzolino, Gizem Imamoglu, and Emre Seli. "Mitochondrial Stress Response Gene Clpp Is Not Required for Granulosa Cell Function." Antioxidants 10, no. 1 (2020): 1. http://dx.doi.org/10.3390/antiox10010001.

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Mitochondrial unfolded protein response (UPRmt) is a highly conserved mechanism, which is activated upon cellular or metabolic stress and aims to help cells maintain homeostasis. CLPP (caseinolytic peptidase P) plays a crucial factor for UPRmt; it promotes the degradation of unfolded mitochondrial proteins. Global germline deletion of Clpp in mice results in female infertility and accelerated follicular depletion. Here, we asked whether CLPP is necessary for granulosa/cumulus cell function. Clppflox/flox mice were generated and crossbred with Cyp19a1-Cre mice to generate mice with granulosa/cu

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Kawashima, Ikkou, Tetsuji Okazaki, Noritaka Noma, Masahide Nishibori, Yasuhisa Yamashita, and Masayuki Shimada. "Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro." REPRODUCTION 136, no. 1 (2008): 9–21. http://dx.doi.org/10.1530/rep-08-0074.

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In this study, we collected follicular fluid, granulosa cells, and cumulus cells from antral follicles at specific time intervals following equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) treatment of gilts. The treatment with eCG increased the production of estrogen coordinately with up-regulated proliferation of granulosa and cumulus cells. eCG also induced the expression ofLHCGRandPGRin cumulus cells and progesterone accumulation was detected in follicular fluid prior to the LH/hCG surge. Moreover, progesterone and progesterone receptor (PGR) were critical for FSH

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Ikeda, S., K. Saeki, H. Imai, and M. Yamada. "Abilities of cumulus and granulosa cells to enhance the developmental competence of bovine oocytes during in vitro maturation period are promoted by midkine; a possible implication of its apoptosis suppressing effects." Reproduction 132, no. 4 (2006): 549–57. http://dx.doi.org/10.1530/rep.1.01066.

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We previously reported that when midkine (MK), a heparin-binding growth differentiation factor was used inin vitromaturation (IVM) culture of bovine cumulus-enclosed oocytes (CEOs), their developmental competence to the blastocyst stage afterin vitrofertilization (IVF) was enhanced and the effect of MK might be mediated by its action upon mural granulosa cells and cumulus cells that closely surround the oocyte. In the present study, when denuded oocytes (DOs) were matured in IVM medium with or without MK (200 ng/ml) in the presence or absence of isolated cumulus cell masses and subjected to IV

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Su, You-Qiang, Koji Sugiura, Qinglei Li, Karen Wigglesworth, Martin M. Matzuk, and John J. Eppig. "Mouse Oocytes Enable LH-Induced Maturation of the Cumulus-Oocyte Complex via Promoting EGF Receptor-Dependent Signaling." Molecular Endocrinology 24, no. 6 (2010): 1230–39. http://dx.doi.org/10.1210/me.2009-0497.

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Abstract LH triggers the maturation of the cumulus-oocyte complex (COC), which is followed by ovulation. These ovarian follicular responses to LH are mediated by epidermal growth factor (EGF)-like growth factors produced by granulosa cells and require the participation of oocyte-derived paracrine factors. However, it is not clear how oocytes coordinate with the EGF receptor (EGFR) signaling to achieve COC maturation. The aim of the present study was to test the hypothesis that oocytes promote the expression of EGFR by cumulus cells, thus enabling them to respond to the LH-induced EGF-like pept

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Salamone, D., M. Catala, A. Gibbons, F. Pereyra Bonnet, and M. Cueto. "70 NON-PLATED GRANULOSA AND CUMULUS CELLS AND FIRST PASSAGE FIBROBLASTS AS NUCLEUS DONOR FOR GOAT CLONING." Reproduction, Fertility and Development 18, no. 2 (2006): 143. http://dx.doi.org/10.1071/rdv18n2ab70.

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Different types of somatic cells have been used as nucleus donors for cloning. Most of them were previously cultured in vitro as a monolayer through several plate passages. The experiment reported here was conducted to study the potential usages of granulosa and cumulus cells for cloning without previous culture as a monolayer. A first-plate-passage fibroblast was also used. Oocytes were aspirated by laparoscopy from Criolla goats and matured in TCM-199 + 5% FCS at 39°C for 24 h. Matured oocytes were denuded by vortexing for 3 min in TL HEPES with 1 mg/mL bovine testis hyaluronidase. Metaphase

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Tas Tuna, Ayca, Havva Kocayigit, Gurkan Demir, et al. "Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats." Journal of Anesthesiology and Reanimation Specialists' Society 31, no. 1 (2023): 50–54. http://dx.doi.org/10.54875/jarss.2023.88942.

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Objective: We aimed to evaluate the potential apoptotic effects of propofol, dexmedetomidine and medetomidine that were used during oocyte retrieval on cumulus cells in rat ovulation induction model. Methods: After the ovulation induction model was created, rats were received dexmedetomidine in Group D, propofol in Group P, medetomidine in Group M. Oocytes collection was performed 10 minutes after the administration of study drugs. For Caspase-3 immunohistochemical evaluation, the staining level was scored in five randomly selected areas and the areas with the highest score were determined. Im

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Russell, D. L. "026. The cumulus matrix in ovulation: inert packaging or active delivery vehicle for the oocyte?" Reproduction, Fertility and Development 17, no. 9 (2005): 69. http://dx.doi.org/10.1071/srb05abs026.

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Preovulatory follicles respond to the LH-surge with a cascade of molecular events. The ovulatory signal initially impinges on the mural granulosa layers triggering rapid tissue morphogenesis and ultimately terminal differentiation of these cells. Mural granulosa cells transiently produce a suite of transcriptional regulators, EGF-like ligands as well the extracellular matrix (ECM) proteoglycan, versican and the protease ADAMTS-1. These act in concert with permissive oocyte signals to induce and organise a complex hyaluronan (HA) rich ECM surrounding the cumulus cells and oocyte. This expanded

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Koike, Hiroshi, Miyuki Harada, Akari Kusamoto, et al. "Notch Signaling Induced by Endoplasmic Reticulum Stress Regulates Cumulus-Oocyte Complex Expansion in Polycystic Ovary Syndrome." Biomolecules 12, no. 8 (2022): 1037. http://dx.doi.org/10.3390/biom12081037.

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Endoplasmic reticulum (ER) stress activated in granulosa cells contributes to the pathophysiology of polycystic ovary syndrome (PCOS). In addition, recent studies have demonstrated that Notch signaling plays multiple roles in the ovary via cell-to-cell interactions. We hypothesized that ER stress activated in granulosa cells of antral follicles in PCOS induces Notch signaling in these cells, and that activated Notch signaling induces aberrant cumulus-oocyte complex (COC) expansion. Expression of Notch2 and Notch-target transcription factors was increased in granulosa cells of PCOS patients and

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Varani, Simona, Julia A. Elvin, Changning Yan, et al. "Knockout of Pentraxin 3, a Downstream Target of Growth Differentiation Factor-9, Causes Female Subfertility." Molecular Endocrinology 16, no. 6 (2002): 1154–67. http://dx.doi.org/10.1210/mend.16.6.0859.

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Abstract The ovulatory process is tightly regulated by endocrine as well as paracrine factors. In the periovulatory period, extensive remodeling of the follicle wall occurs to allow the extrusion of the oocyte and accompanying cumulus granulosa cells. Growth differentiation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15) are secreted members of the TGFβ superfamily that are expressed beginning in the oocyte of small primary follicles and through ovulation. Besides its critical role as a growth and differentiation factor during early folliculogenesis, GDF-9 also acts as a paracrine

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Sršeň, Vlastimil, Jaroslav Kalous, Eva Nagyova, Peter šutovský, W. Allan King, and Jan Motlik. "Effects of follicle-stimulating hormone, bovine somototrophin and okadaic acid on cumulus expansion and nuclear maturation of Blue fox (Alopex lagopus) oocytes in vitro." Zygote 6, no. 4 (1998): 299–309. http://dx.doi.org/10.1017/s0967199498000252.

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The meiotic competence and meiosis resumption of Blue fox (Alopex lagopus) oocytes from anoestrous animals were followed. Oocyte–cumulus complexes (OCC) were cultured in modified TC 199 medium with or without FSH, recombinant bovine somatotrophin (bST) and okadaic acid (OA). The results showed that oocytes less than 100 μm in diameter did not achieve germinal vesicle breakdown (GFBD) by 72 h of culture, which indicates their meiotic incompetence. Oocytes larger than 100 µm in diameter underwent GVBD after 48 h of culture (27%) and reached metaphase II (MII) after 72 and 96 h (20% and 27%) in c

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Kordus, Richard J., and Holly A. LaVoie. "Granulosa cell biomarkers to predict pregnancy in ART: pieces to solve the puzzle." Reproduction 153, no. 2 (2017): R69—R83. http://dx.doi.org/10.1530/rep-16-0500.

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Cumulus and mural granulosa cells of the ovarian follicle surround and interact with the developing oocyte. These follicular cells reflect the oocyte’s overall health and may indicate subsequent developmental competence of embryos. Biomarkers of granulosa cells associated with individual oocytes could potentially be used in assisted reproduction to indicate which embryos have the best chance of implanting in the uterus and completing gestation. In this review, we have performed a comprehensive assessment of the recent literature for human cumulus and mural granulosa cell mRNA biomarkers as the

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Pacella, L., D. Zander-Fox, T. Hussein, T. Fullston, and M. Lane. "131. SIRT3 IN OVARIAN CELLS IS ALTERED BY MATERNAL AGE AND OVARIAN RESERVE." Reproduction, Fertility and Development 22, no. 9 (2010): 49. http://dx.doi.org/10.1071/srb10abs131.

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Maternal age and reduced AMH levels affect the follicular environment and consequently oocyte viability. The Sirtuin family of protein deacetylases are able to regulate various cellular functions involved in the ageing process in other tissues. In particular, SIRT3 is related to longevity in several cell types and regulates mitochondrial function, however, its presence and role in ovarian cells remains unknown. This study therefore, investigated the presence of SIRT3 in granulosa and cumulus cells, from patients undergoing IVF, and determined the impact of maternal age and low AMH on SIRT3 lev

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Szołtys, M., Z. Tabarowski, and A. Pawlik. "Apoptosis of postovulatory cumulus granulosa cells of the rat." Anatomy and Embryology 202, no. 6 (2000): 523–29. http://dx.doi.org/10.1007/s004290000122.

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Chan, Carina C. W., Vincent W. S. Liu, Estella Y. L. Lau, William S. B. Yeung, Ernest H. Y. Ng, and Pak-Chung Ho. "Mitochondrial DNA deletion in granulosa and cumulus oophorus cells." Fertility and Sterility 85, no. 3 (2006): 780–82. http://dx.doi.org/10.1016/j.fertnstert.2005.08.042.

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Caillaud, Maud, та Nadine Gérard. "In vivo and in vitro effects of interleukin-1β on equine oocyte maturation and on steroidogenesis and prostaglandin synthesis in granulosa and cumulus cells". Reproduction, Fertility and Development 21, № 2 (2009): 265. http://dx.doi.org/10.1071/rd08046.

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We analysed the effect of interleukin-1 on oocyte maturation and on steroid and prostaglandin production by equine granulosa and cumulus cells. In Experiment 1, interleukin-1β (IL-1β) was injected into the growing dominant follicle, which was punctured 38 h later. Follicular fluid was assayed for steroids and prostaglandin-F2α (PGF2α). Granulosa cells were analysed for 3β-hydroxysteroid dehydrogenase (3β-HSD), progesterone receptor (PR), cyclooxygenase 1 and 2 (Cox 1 and Cox 2) and steroidogenic acute regulatory protein (StAR) mRNAs. In Experiment 2, cumulus–oocyte complexes (COCs) were collec

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Khurchabilig, Atchalalt, Akane Sato, Shiori Ashibe, Asuka Hara, Rika Fukumori та Yoshikazu Nagao. "Expression levels of FSHR, IGF1R, CYP11al and HSD3β in cumulus cells can predict in vitro developmental competence of bovine oocytes". Zygote 28, № 5 (2020): 425–31. http://dx.doi.org/10.1017/s0967199420000283.

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SummaryThe efficiency of in vitro embryo production technologies would be improved by the development of suitable non-invasive biomarkers that allow the selection of good quality cumulus–oocyte complexes (COCs). The present study used whole, single oocyte culture to investigate whether the expression levels of follicle-stimulating hormone receptor (FSHR), insulin-like factor 1 receptor (IGF1R) and three steroidogenesis-related enzymes (CYP11al, CYP19al and HSD3β) in cumulus cells reflected the developmental competence of COCs. Cumulus cells were collected from single COCs before maturation cul

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Yuningsih, Tita, Budi Wiweko, Arief Boediono, Ina Gunawati, and Nining Handayani. "Utilisation of Oocyte Diameter as a Non-invasive Indicator of Oocyte and Embryo Quality: Investigating Protein Kinase B Protein Expression in Cumulus–Granulosa Cells and Embryo Morphokinetics." Journal of Human Reproductive Sciences 17, no. 3 (2024): 185–92. http://dx.doi.org/10.4103/jhrs.jhrs_67_24.

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Background: Oocyte diameter is crucial for initiating meiosis and supporting the maturation capability, a prerequisite for successful fertilisation and high-quality embryo development. While numerous studies have explored the correlation between oocyte diameter and embryo development capacity in animal models, limited research appears concerning human oocytes. Aim: This study aims to ascertain whether oocyte diameter can serve as a reliable predictor of oocyte and embryo quality, achieved through its correlation with protein kinase B (Akt) protein levels on cumulus–granulosa cells and blastocy

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Torrealday, Saioa, Maria D. Lalioti, Ozlem Guzeloglu-Kayisli, and Emre Seli. "Characterization of the Gonadotropin Releasing Hormone Receptor (GnRHR) Expression and Activity in the Female Mouse Ovary." Endocrinology 154, no. 10 (2013): 3877–87. http://dx.doi.org/10.1210/en.2013-1341.

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GnRH agonists (GnRHa) are increasingly used for fertility preservation in women undergoing gonadotoxic chemotherapy. However, the protective mechanisms of action for these compounds have not yet been elucidated. In this study, we aimed to determine whether GnRHa have a direct effect on ovarian granulosa cells. GnRH receptor (GnRHR) expression was determined in mouse somatic and gonadal tissues including granulosa/cumulus cells and oocytes using quantitative RT-PCR and immunohistochemistry. Granulosa cells were isolated from mouse ovaries primed with pregnant mare serum gonadotropin. Response t

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Chen, Na, Yong Zhang, Yuhan Tian, Shumei Wu, Fei Gao, and Xiaolong Yuan. "Deciphering Cellular Heterogeneity and Communication Patterns in Porcine Antral Follicles by Single-Cell RNA Sequencing." Animals 13, no. 19 (2023): 3019. http://dx.doi.org/10.3390/ani13193019.

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The antral follicle stage is a critical period in mammalian oocyte maturation, marked by complex interactions between oocyte development and neighboring granulosa cells. Understanding the heterogeneity and communication patterns of granulosa cells within antral follicles is crucial for deciphering their roles in follicle development and oocyte maturation. Here, we employed single-cell RNA-sequencing to explore the molecular and cellular characteristics of porcine antral follicles. Our analysis revealed distinct subpopulations within mural and cumulus granulosa cells, indicating diverse cellula

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Cannon, Jennifer D., Mary Cherian-Shaw, and Charles L. Chaffin. "Proliferation of Rat Granulosa Cells during the Periovulatory Interval." Endocrinology 146, no. 1 (2005): 414–22. http://dx.doi.org/10.1210/en.2004-0581.

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Granulosa cell proliferation during luteinization and terminal differentiation has historically been assumed to decline rapidly after an ovulatory stimulus. In contrast, terminal differentiation in other cell types has recently been associated with a transient increase in proliferation, suggesting that this may occur in the ovarian follicle. The goal of the current study was to test the hypothesis that an ovulatory stimulus to rats results in additional granulosa cell proliferation before cell cycle arrest. Immature rats were given a single injection of pregnant mare serum gonadotropin (PMSG)

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Kang, Woojin, Eri Ishida, Mitsuyoshi Amita, et al. "Trehalose Suppresses Lysosomal Anomalies in Supporting Cells of Oocytes and Maintains Female Fertility." Nutrients 14, no. 10 (2022): 2156. http://dx.doi.org/10.3390/nu14102156.

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Supporting cells of oocytes, i.e., cumulus cells, control oocyte quality, which determines fertilization success. Therefore, the transformation of mature and immature cumulus cells (MCCs and ICCs, respectively) into dysmature cumulus cells (DCCs) with dead characteristics deteriorates oocyte quality. However, the molecular basis for this transformation remains unclear. Here, we explored the link between autophagic decline and cumulus transformation using cumulus cells from patients with infertility, female mice, and human granulosa cell-derived KGN cell lines. When human cumulus cells were lab

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Schuetz, AW, DG Whittingham, and R. Snowden. "Alterations in the cell cycle of mouse cumulus granulosa cells during expansion and mucification in vivo and in vitro." Reproduction, Fertility and Development 8, no. 6 (1996): 935. http://dx.doi.org/10.1071/rd9960935.

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The cell cycle characteristics of mouse cumulus granulosa cells were determined before, during and following their expansion and mucification in vivo and in vitro. Cumulus-oocyte complexes (COC) were recovered from ovarian follicles or oviducts of prepubertal mice previously injected with pregnant mare serum gonadotrophin (PMSG) or a mixture of PMSG and human chorionic gonadotrophin (PMSG+hCG) to synchronize follicle differentiation and ovulation. Cell cycle parameters were determined by monitoring DNA content of cumulus cell nuclei, collected under rigorously controlled conditions, by flow cy

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Zhou, Cheng-Jie, Sha-Na Wu, Jiang-Peng Shen, et al. "The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice." PeerJ 4 (March 3, 2016): e1761. http://dx.doi.org/10.7717/peerj.1761.

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Cumulus cells are a group of closely associated granulosa cells that surround and nourish oocytes. Previous studies have shown that cumulus cells contribute to oocyte maturation and fertilization through gap junction communication. However, it is not known how this gap junction signaling affectsin vivoversusin vitromaturation of oocytes, and their subsequent fertilization and embryonic development following insemination. Therefore, in our study, we performed mouse oocyte maturation and insemination usingin vivo- orin vitro-matured oocyte-cumulus complexes (OCCs, which retain gap junctions betw

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Stavros, Sofoklis, Anastasios Potiris, Despoina Mavrogianni, et al. "Exploring the Potential Impact of SERPINE Gene Expression in Cumulus Cells During Fertility Treatments: A Single Center Study." Clinics and Practice 15, no. 5 (2025): 83. https://doi.org/10.3390/clinpract15050083.

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Background/Objectives: Cumulus cells have been proposed to be indicators of oocyte quality. In this study, oocyte cumulus cells were analyzed for SERPINE gene expression. High SERPINE gene expression in cumulus cells is associated with reduced oocyte maturity. However, high mRNA levels in granulosa cells are associated with follicles that result in pregnancy. This study aimed to evaluate SERPINE gene expression in cumulus cells across different ovarian stimulation protocols and its potential impact on follicle number, oocyte maturity, and embryo quality. Methods: The sample of the study consis

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Dehghan, Zeinab, Samira Mohammadi-Yeganeh, and Mohammad Salehi. "MiRNA-155 regulates cumulus cells function, oocyte maturation, and blastocyst formation." Biology of Reproduction 103, no. 3 (2020): 548–59. http://dx.doi.org/10.1093/biolre/ioaa098.

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Abstract Numerous oocytes are retrieved during in vitro fertilization from patients with polycystic ovary syndrome (PCOS). The poor quality of these oocytes leads to lower fertilization and decreases in cleavage and implantation. MiR-155 is one of the microRNA (miRNA) that is increased in serum and granulosa cells of PCOS patients. In this study, we investigate the effects of miR-155 expression and its target genes on oocyte maturation and embryo development. We used the calcium phosphate protocol to transfect vectors that contained miR-155 or miR-off 155 and alone eGFP into cumulus oophorus c

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Wu, Linda Lin-Yan, Kylie R. Dunning, Xing Yang, et al. "High-Fat Diet Causes Lipotoxicity Responses in Cumulus–Oocyte Complexes and Decreased Fertilization Rates." Endocrinology 151, no. 11 (2010): 5438–45. http://dx.doi.org/10.1210/en.2010-0551.

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In obesity, accumulation of lipid in nonadipose tissues, or lipotoxicity, is associated with endoplasmic reticulum (ER) stress, mitochondrial dysfunction, and ultimately apoptosis. We have previously shown that obese women have increased triglycerides in follicular fluid; thus, the present study examined whether high-fat diet–induced obesity causes lipotoxicity in granulosa cells and the cumulus–oocyte complex (COC). Oocytes of mice fed a high-fat diet had dramatically increased lipid content and reduced mitochondrial membrane potential compared to those of mice fed a control diet. COCs from m

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Machado, M. F., V. M. Portela, C. A. Price, et al. "Regulation and action of fibroblast growth factor 17 in bovine follicles." Journal of Endocrinology 202, no. 3 (2009): 347–53. http://dx.doi.org/10.1677/joe-09-0145.

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Fibroblast growth factor 17 (FGF17) is a member of the FGF8 subfamily that appears to be relevant to folliculogenesis and oogenesis, as the prototype member FGF8 is an oocyte-derived protein that signals to cumulus cells. FGF8 has structural and receptor-binding similarities to FGF17, whose expression in the ovary has not been reported. In this study, we demonstrate localization of FGF17 protein to the oocyte of preantral follicles, and to the oocyte and granulosa cells of antral follicles. Real-time PCR demonstrated the presence of mRNA in oocytes and, to a lesser extent, in granulosa and the

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Eo, Jinwon, Kyuyong Han, Kenneth M Murphy, Haengseok Song, and Hyunjung Jade Lim. "Etv5, an ETS transcription factor, is expressed in granulosa and cumulus cells and serves as a transcriptional regulator of the cyclooxygenase-2." Journal of Endocrinology 198, no. 2 (2008): 281–90. http://dx.doi.org/10.1677/joe-08-0142.

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Etv4, Etv1, and Etv5 are members of Etv4 subfamily of E26 transformation-specific (Ets) transcription factors that are known to influence a host of biological processes. We previously showed that Etv5, expressed in Sertoli cells, plays a crucial role in maintaining spermatogonial stem cell niche in the mouse testis. However, it is not yet known whether Etv4 family members are expressed in the ovary or play any role in ovarian functions. Here, we show that Etv5 and Etv4 are expressed in mouse ovaries in granulosa and cumulus cells during folliculogenesis. Both Etv5 and Etv4 mRNAs are also detec

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Sasseville, M., L. J. Ritter, T. Nguyen, D. G. Mottershead, D. L. Russell, and R. B. Gilchrist. "134. EPIDERMAL GROWTH FACTOR RECEPTOR/MAPK3/1 PATHWAY CROSS-TALK ENABLES GROWTH DIFFERENTIATION FACTOR 9 TO SIGNAL THROUGH SMAD2/3 IN MOUSE GRANULOSA CELLS." Reproduction, Fertility and Development 21, no. 9 (2009): 53. http://dx.doi.org/10.1071/srb09abs134.

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Oocyte-secreted growth differentiation factor 9 (GDF9) plays a critical role throughout folliculogenesis. It has been shown to control many functions of granulosa cells, including gene expression, steroidogenesis and proliferation. This study investigates the cellular requirements that allow GDF9 to act on granulosa cells. Our results showed that GDF9 (20 ng/ml)-stimulated mouse granulosa cells 3H-thymidine incorporation was inhibited by a type 1 receptor Alk4/5/7 inhibitor (SB431542, 5 μM), by an epidermal growth factor (EGF) receptor inhibitor (AG1478, 5μM) and a MEK1 inhibitor (U0126, 10 μM

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Richards, J. "037. IMMUNE-LIKE MECHANISMS ASSOCIATED WITH OVULATION." Reproduction, Fertility and Development 21, no. 9 (2009): 10. http://dx.doi.org/10.1071/srb09abs037.

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Ovulation is the unique biological process by which a mature oocyte and surrounding somatic cells, the cumulus cell-oocyte complex (COC), are released from the surface of the ovary into the oviduct for transport and fertilization. Ovulation is similar to an inflammatory response: the follicles become hyperemic, produce prostaglandins and synthesize a hyaluronan-rich extracellular matrix. However, this view of ovulation may be too restrictive and need to be broadened to encompass the innate immune cell surveillance response system. This hypothesis is being proposed because ovarian granulosa cel

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Emori, Chihiro, Haruka Ito, Wataru Fujii, Kunihiko Naito, and Koji Sugiura. "Oocytes suppress FOXL2 expression in cumulus cells in mice†." Biology of Reproduction 103, no. 1 (2020): 85–93. http://dx.doi.org/10.1093/biolre/ioaa054.

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Abstract Cumulus cells and mural granulosa cells (MGCs) play distinct roles during follicular development, and normal development of these cell lineages is critical for the female fertility. Transcriptomic diversification between the two cell lineages is obviously a critical mechanism for their functional diversification; however, the transcriptional regulators responsible for this event have not been fully defined. In this study, we sought to identify key transcriptional regulators responsible for the differential gene expression between the two cell lineages. In silico analysis of transcript

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Buratini, J., and C. A. Price. "Follicular somatic cell factors and follicle development." Reproduction, Fertility and Development 23, no. 1 (2011): 32. http://dx.doi.org/10.1071/rd10224.

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Considerable attention is currently paid to oocyte-derived secreted factors that act upon cumulus and granulosa cells. Also important for follicle development are somatic cell-derived secreted factors. This is illustrated by the ability of granulosa cell-derived Kit ligand (KITL) to promote primordial follicle activation, and the loss of follicle development that accompanies KITL gene disruption. This review summarises our current understanding of somatic cell factors during both preantral and antral follicle growth, involving not only signalling from granulosa cells to the oocyte, but also si

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Ruvolo, Giovanni, Domenica Matranga, Maria Magdalena Barreca, and Liana Bosco. "AKT, p-AKT, ERK1/2 and p-ERK1/2 in Mural Granulosa Cells Are Not Correlated to Different Ovarian Stimulation Protocols in Patients Undergoing Assisted Reproductive Treatment." Life 14, no. 5 (2024): 554. http://dx.doi.org/10.3390/life14050554.

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(1) Background: In this paper we aim to study the relationship between the expression levels of molecules involved in apoptotic/survival pathways, considered as molecular markers of oocyte competence (i.e., AKT, p-AKT, ERK1/2, and p-ERK1/2) in mural granulosa cells (MGCs) and the administration of r-FSH alone or combined with exogenous r-LH, in ovarian stimulation protocol. Moreover, we aim to evaluate oocyte competence by comparing normally cleaved embryos that were transferred in the uterus, with embryos that were arrested during in vitro culture. (2) Methods: The study included 34 normo-res

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González-Fernández, L., M. J. Sánchez-Calabuig, M. G. Alves, et al. "Expanded equine cumulus–oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus–oocyte complexes." Reproduction, Fertility and Development 30, no. 2 (2018): 297. http://dx.doi.org/10.1071/rd16441.

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Equine cumulus–oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This difference could be related to divergent glucose metabolism. To test this hypothesis in the present study, eCOCs, cCOCs and expanded or compact mural granulosa cells (EC and CC respectively) were matured in vitro for 30 h, at which time maturation rate, glucose metabolism and the expression of genes involved in glucose transport, glycolysis, apoptosis and meiotic competence were determined. There were significant differences between eCOCs and cCOCs in maturation

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Li, Qinglei, Stephanie A. Pangas, Carolina J. Jorgez, Jonathan M. Graff, Michael Weinstein, and Martin M. Matzuk. "Redundant Roles of SMAD2 and SMAD3 in Ovarian Granulosa Cells In Vivo." Molecular and Cellular Biology 28, no. 23 (2008): 7001–11. http://dx.doi.org/10.1128/mcb.00732-08.

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ABSTRACT Transforming growth factor β (TGF-β) superfamily members are critical in maintaining cell growth and differentiation in the ovary. Although signaling of activins, TGF-βs, growth differentiation factor 9, and nodal converge preferentially to SMAD2 and SMAD3, the in vivo functions and redundancy of these SMADs in the ovary and female reproduction remain largely unidentified. To circumvent the deleterious phenotypic aspects of ubiquitous deletion of Smad2 and Smad3, a conditional knockout strategy was formulated to selectively inactivate Smad2, Smad3, or both Smad2 and Smad3 in ovarian g

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