Relevant bibliographies by topics / GroESL

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  2. Dissertations / Theses
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Academic literature on the topic 'GroESL'

Author: Grafiati
Published: 7 September 2021
Last updated: 18 February 2022

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Journal articles on the topic "GroESL"

1

Bittner, Alycia N., Amanda Foltz, and Valerie Oke. "Only One of Five groEL Genes Is Required for Viability and Successful Symbiosis in Sinorhizobium meliloti." Journal of Bacteriology 189, no. 5 (2006): 1884–89. http://dx.doi.org/10.1128/jb.01542-06.

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ABSTRACT Many bacterial species contain multiple copies of the genes that encode the chaperone GroEL and its cochaperone, GroES, including all of the fully sequenced root-nodulating bacteria that interact symbiotically with legumes to generate fixed nitrogen. In particular, in Sinorhizobium meliloti there are four groESL operons and one groEL gene. To uncover functional redundancies of these genes during growth and symbiosis, we attempted to construct strains containing all combinations of groEL mutations. Although a double groEL1 groEL2 mutant cannot be constructed, we demonstrate that the quadruple groEL1 groESL3 groEL4 groESL5 and groEL2 groESL3 groEL4 groESL5 mutants are viable. Therefore, like E. coli and other species, S. meliloti requires only one groEL gene for viability, and either groEL1 or groEL2 will suffice. The groEL1 groESL5 double mutant is more severely affected for growth at both 30°C and 40°C than the single mutants, suggesting overlapping functions in stress response. During symbiosis the quadruple groEL2 groESL3 groEL4 groESL5 mutant acts like the wild type, but the quadruple groEL1 groESL3 groEL4 groESL5 mutant acts like the groEL1 single mutant, which cannot fully induce nod gene expression and forms ineffective nodules. Therefore, the only groEL gene required for symbiosis is groEL1. However, we show that the other groE genes are expressed in the nodule at lower levels, suggesting minor roles during symbiosis. Combining our data with other data, we conclude that groESL1 encodes the housekeeping GroEL/GroES chaperone and that groESL5 is specialized for stress response.
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Bittner, Alycia N., and Valerie Oke. "Multiple groESL Operons Are Not Key Targets of RpoH1 and RpoH2 in Sinorhizobium meliloti." Journal of Bacteriology 188, no. 10 (2006): 3507–15. http://dx.doi.org/10.1128/jb.188.10.3507-3515.2006.

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ABSTRACT Among the rhizobia that establish nitrogen-fixing nodules on the roots of host plants, many contain multiple copies of genes encoding the sigma factor RpoH and the chaperone GroEL/GroES. In Sinorhizobium meliloti there are two rpoH genes, four groESL operons, and one groEL gene. rpoH1 mutants are defective for growth at high temperature and form ineffective nodules, rpoH1 rpoH2 double mutants are unable to form nodules, and groESL1 mutants form ineffective nodules. To explore the roles of RpoH1 and RpoH2, we identified mutants that suppress both the growth and nodulation defects. These mutants do not suppress the nitrogen fixation defect. This implies that the functions of RpoH1 during growth and RpoH1/RpoH2 during the initiation of symbiosis are similar but that there is a different function of RpoH1 needed later during symbiosis. We showed that, unlike in Escherichia coli, overexpression of groESL is not sufficient to bypass any of the RpoH defects. Under free-living conditions, we determined that RpoH2 does not control expression of the groE genes, and RpoH1 only controls expression of groESL5. Finally, we completed the series of groE mutants by constructing groESL3 and groEL4 mutants and demonstrated that they do not display symbiotic defects. Therefore, the only groESL operon required by itself for symbiosis is groESL1. Taken together, these results suggest that GroEL/GroES production alone cannot explain the requirements for RpoH1 and RpoH2 in S. meliloti and that there must be other crucial targets.
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Karunakaran, Karuna P., Yasuyuki Noguchi, Timothy D. Read, et al. "Molecular Analysis of the Multiple GroEL Proteins of Chlamydiae." Journal of Bacteriology 185, no. 6 (2003): 1958–66. http://dx.doi.org/10.1128/jb.185.6.1958-1966.2003.

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ABSTRACT Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid sequences of the three groEL genes with those of other organisms showed high homology only for groEL1, although comparison of critical amino acid residues that are required for polypeptide binding of the Escherichia coli chaperonin GroEL revealed substantial conservation in all three chlamydial GroELs. This was further supported by three-dimensional structural predictions. All three genes are expressed constitutively throughout the developmental cycle of Chlamydia trachomatis, although groEL1 is expressed at much higher levels than are groEL2 and groEL3. Transcription of groEL1, but not groEL2 and groEL3, was elevated when HeLa cells infected with C. trachomatis were subjected to heat shock. Western blot analysis with polyclonal antibodies raised against recombinant GroEL1, GroEL2, and GroEL3 demonstrated the presence of the three proteins in C. trachomatis elementary bodies, with GroEL1 being present in the largest amount. Only C. trachomatis groEL1 and groES together complemented a temperature-sensitive E. coli groEL mutant. Complementation did not occur with groEL2 or groEL3 alone or together with groES. The role for each of the three GroELs in the chlamydial developmental cycle and in disease pathogenesis requires further study.
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Chen, Hsiao-Jan, Jui-Chang Tsai, Tsung-Chain Chang, et al. "PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences." Journal of Medical Microbiology 57, no. 4 (2008): 432–38. http://dx.doi.org/10.1099/jmm.0.47628-0.

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The sequence diversity of groESL genes among Streptococcus bovis group isolates was analysed, including five reference strains and 36 clinical isolates. Phylogenetic analysis of the groES and groEL sequences showed that the isolates that belonged to the same species or subspecies usually clustered together. The intergenic spacer region between groES and groEL was variable in size (67–342 bp) and sequence and appeared to be a unique marker for species or subspecies determination. Sequence similarities of the groESL genes among species and subspecies ranged from 84.2 to 99.0 % in groES, and from 88.0 to 99.0 % in groEL. Based on the sequences determined, a Streptococcus bovis group-specific PCR assay was developed, which may provide an alternative means of distinguishing the bovis group from other viridans streptococci. Restriction digestion of the amplicon with AclI further differentiated the species and subspecies.
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Gahan, Cormac G. M., James O'Mahony, and Colin Hill. "Characterization of the groESL Operon inListeria monocytogenes: Utilization of Two Reporter Systems (gfp and hly) for Evaluating In Vivo Expression." Infection and Immunity 69, no. 6 (2001): 3924–32. http://dx.doi.org/10.1128/iai.69.6.3924-3932.2001.

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ABSTRACT The ability of intracellular pathogens to sense and adapt to the hostile environment of the host is an important factor governing virulence. We have sequenced the operon encoding the major heat shock proteins GroES and GroEL in the gram-positive food-borne pathogenListeria monocytogenes. The operon has a conserved orientation in the order groES groEL. Upstream ofgroES and in the opposite orientation is a gene encoding a homologue of the Bacillus subtilis protein YdiL, while downstream of groEL is a gene encoding a putative bile hydrolase. We used both reverse transcriptase-PCR (RT-PCR) and transcriptional fusions to the UV-optimized Aequorea victoria green fluorescent protein (GFPUV) to analyze expression of groESL under various environmental stress conditions, including heat shock, ethanol stress, and acid shock, and during infection of J774 mouse macrophage cells. Strains harboring GFPUV transcriptional fusions to the promoter region ofgroESL demonstrated a significant increase in fluorescence following heat shock that was detected by both fluorimetry and fluorescence microscopy. Using both RT-PCR and GFP technology we detected expression of groESL following internalization by J774 cells. Increased intracellular expression of dnaK was also determined using RT-PCR. We have recently described a system which utilizes L. monocytogenes hemolysin as an in vivo reporter of gene expression within the host cell phagosome (C. G. M. Gahan and C. Hill, Mol. Microbiol. 36:498–507, 2000). In this study a strain was constructed in which hemolysin expression was placed under the control of the groESL promoter. In this strain hemolysin expression during infection also confirms transcription from the groESL promoter during J774 and murine infection, albeit at lower levels than the known virulence factorplcA.
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Susin, Michelle F., Humberto R. Perez, Regina L. Baldini, and Suely L. Gomes. "Functional and Structural Analysis of HrcA Repressor Protein from Caulobacter crescentus." Journal of Bacteriology 186, no. 20 (2004): 6759–67. http://dx.doi.org/10.1128/jb.186.20.6759-6767.2004.

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ABSTRACT A large number of bacteria regulate chaperone gene expression during heat shock by the HrcA-CIRCE system, in which the DNA element called CIRCE serves as binding site for the repressor protein HrcA under nonstress conditions. In Caulobacter crescentus, the groESL operon presents a dual type of control. Heat shock induction is controlled by a σ32-dependent promoter and the HrcA-CIRCE system plays a role in regulation of groESL expression under physiological temperatures. To study the activity of HrcA in vitro, we purified a histidine-tagged version of the protein, and specific binding to the CIRCE element was obtained by gel shift assays. The amount of retarded DNA increased significantly in the presence of GroES/GroEL, suggesting that the GroE chaperonin machine modulates HrcA activity. Further evidence of this modulation was obtained using lacZ transcription fusions with the groESL regulatory region in C. crescentus cells, producing different amounts of GroES/GroEL. In addition, we identified the putative DNA-binding domain of HrcA through extensive protein sequence comparison and constructed various HrcA mutant proteins containing single amino acid substitutions in or near this region. In vitro and in vivo experiments with these mutated proteins indicated several amino acids important for repressor activity.
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Tsugawa, Hitoshi, Humie Ito, Miho Ohshima, and Yoshio Okawa. "Cell adherence-promoted activity of Plesiomonas shigelloides GroEL." Journal of Medical Microbiology 56, no. 1 (2007): 23–29. http://dx.doi.org/10.1099/jmm.0.46766-0.

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Previously, it has been demonstrated that the invasion of Caco-2 cells by Plesiomonas shigelloides induces apoptotic cell death. Therefore, the attachment to and colonization of eukaryotic intestinal host cells by P. shigelloides are important steps in causing pathogenicity. In this study, the participation of P. shigelloides GroEL in the attachment of P. shigelloides was examined. The groESL operon of P. shigelloides was isolated by PCR. The nucleotide sequence of the groESL operon of P. shigelloides revealed two ORFs of 294 nucleotides for groES and 1647 nucleotides for groEL. Cell fractionation and immunostaining experiments suggested that the GroEL of P. shigelloides was associated with the bacterial cell surface. The expression of the groEL gene was upregulated during the attachment and apoptosis-induction stages, and the expression of the protein was also induced during the attachment stage. Furthermore, GroEL efficiently promoted the attachment of P. shigelloides to Caco-2 cells, as measured by a FACSCalibur flow cytometer. These results demonstrated that GroEL has a positive influence on the attachment of P. shigelloides to Caco-2 cells.
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Baldini, Regina Lúcia, Marcelo Avedissian, and Suely Lopes Gomes. "The CIRCE Element and Its Putative Repressor Control Cell Cycle Expression of the Caulobacter crescentus groESLOperon." Journal of Bacteriology 180, no. 7 (1998): 1632–41. http://dx.doi.org/10.1128/jb.180.7.1632-1641.1998.

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ABSTRACT The groESL operon is under complex regulation inCaulobacter crescentus. In addition to strong induction after exposure to heat shock, under physiological growth conditions, its expression is subject to cell cycle control. Transcription and translation of the groE genes occur primarily in predivisional cells, with very low levels of expression in stalked cells. The regulatory region of groESL contains both a ς32-like promoter and a CIRCE element. Overexpression ofC. crescentus ς32 gives rise to higher levels of GroEL and increased levels of the groESL transcript coming from the ς32-like promoter. Site-directed mutagenesis in CIRCE has indicated a negative role for thiscis-acting element in the expression of groESLonly at normal growth temperatures, with a minor effect on heat shock induction. Furthermore, groESL-lacZ transcription fusions carrying mutations in CIRCE are no longer cell cycle regulated. Analysis of an hrcA null strain, carrying a disruption in the gene encoding the putative repressor that binds to the CIRCE element, shows constitutive synthesis of GroEL throughout theCaulobacter cell cycle. These results indicate a negative role for the hrcA gene product and the CIRCE element in the temporal control of the groESL operon.
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Kumar, C. M. Santosh, Garima Khare, C. V. Srikanth, Anil K. Tyagi, Abhijit A. Sardesai, and Shekhar C. Mande. "Facilitated Oligomerization of Mycobacterial GroEL: Evidence for Phosphorylation-Mediated Oligomerization." Journal of Bacteriology 191, no. 21 (2009): 6525–38. http://dx.doi.org/10.1128/jb.00652-09.

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ABSTRACT The distinctive feature of the GroES-GroEL chaperonin system in mediating protein folding lies in its ability to exist in a tetradecameric state, form a central cavity, and encapsulate the substrate via the GroES lid. However, recombinant GroELs of Mycobacterium tuberculosis are unable to act as effective molecular chaperones when expressed in Escherichia coli. We demonstrate here that the inability of M. tuberculosis GroEL1 to act as a functional chaperone in E. coli can be alleviated by facilitated oligomerization. The results of directed evolution involving random DNA shuffling of the genes encoding M. tuberculosis GroEL homologues followed by selection for functional entities suggested that the loss of chaperoning ability of the recombinant mycobacterial GroEL1 and GroEL2 in E. coli might be due to their inability to form canonical tetradecamers. This was confirmed by the results of domain-swapping experiments that generated M. tuberculosis-E. coli chimeras bearing mutually exchanged equatorial domains, which revealed that E. coli GroEL loses its chaperonin activity due to alteration of its oligomerization capabilities and vice versa for M. tuberculosis GroEL1. Furthermore, studying the oligomerization status of native GroEL1 from cell lysates of M. tuberculosis revealed that it exists in multiple oligomeric forms, including single-ring and double-ring variants. Immunochemical and mass spectrometric studies of the native M. tuberculosis GroEL1 revealed that the tetradecameric form is phosphorylated on serine-393, while the heptameric form is not, indicating that the switch between the single- and double-ring variants is mediated by phosphorylation.
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Inokuma, Hisashi, Kaori Fujii, Masaru Okuda, et al. "Determination of the Nucleotide Sequences of Heat Shock Operon groESL and the Citrate Synthase Gene (gltA) of Anaplasma (Ehrlichia) platys for Phylogenetic and Diagnostic Studies." Clinical and Vaccine Immunology 9, no. 5 (2002): 1132–36. http://dx.doi.org/10.1128/cdli.9.5.1132-1136.2002.

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ABSTRACT The 1,670-bp nucleotide sequence of the heat shock operon groESL and the 1,236-bp sequence of the citrate synthase gene (gltA) of Anaplasma (Ehrlichia) platys were determined. The topology of the groEL- and gltA-based phylogenetic tree was similar to that derived from 16S rRNA gene analyses with distances. Both groESL- and gltA-based PCRs specific to A. platys were also developed based upon the alignment data.
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Dissertations / Theses on the topic "GroESL"

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Baldini, Regina Lúcia. "Expressão do operon de choque térmico groESL durante o ciclo celular de Caulobacter crescentus." Universidade de São Paulo, 1999. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-30052008-113113/.

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Caulobacter crescentus é uma bactéria Gram-negativa de vida livre cujo ciclo celular depende de eventos de diferenciação celular. A célula pré-divisional assimétrica dá origem a duas células filhas morfológica e funcionalmente distintas: a célula-talo e a célula móvel. A expressão do operon groESL é regulada por choque térmico e durante o ciclo celular a temperaturas normais, sendo a transcrição máxima na célula pré-divisional, com níveis baixos na célula-talo. Numa linhagem superexpressando &#963;32, os níveis do mRNA e da proteína GroEL estão aumentados, indicando que a transcrição ocorre a partir de um promotor ativado por &#963;32. A região regulatória também apresenta uma sequência repetida invertida, CIRCE, em que mutações de ponto aumentam a transcrição apenas a temperaturas normais de crescimento, indicando o papel inibitório desse elemento. Fusões de transcrição groESL-/acZ com mutações em CIRCE deixam de apresentar regulação temporal, bem como a síntese de GroEL numa linhagem hrcA-, em que o gene codificando o provável repressor que se liga em CIRCE está interrompido. Estes resultados indicam que o sistem CIRCE/HrcA está envolvido com a regulação da expressão de groESL durante o ciclo celular. Tentativas de se construir uma linhagem com groEL interrompido não tiveram sucesso, indicando ser este um gene essencial em todas as temperaturas. Um mutante condicional de groESL foi construído por recombinação homóloga e, em condições restritivas, o crescimento é inibido, os níveis de DnaK aumentam e as células se tomam filamentosas, porém não foi observada lise celular. As proteínas essenciais que são dependentes de GroEL/GroES para atingirem sua conformação funcional ainda não foram determinadas.<br>Caulobacter crescentus is an aquatic free-living Gram-negative bacterium whose cell cycle depends on cell differentiation. The asymmetrical predivisional cell gives rise to two morphologically and functionally different daughter cells: the swarmer cell an the stalked cell. The expression of the groESL operon is induced by heat shock and is cell cycle controlled at normal temperatures, with maximal transcription in the predivisional cell and very low levels in the stalked cell. In this work, it was demonstrated that, in a strain overexpressing &#963;32, the levels of groESL transcripts and the synthesis of GroEL are increased, confirming that this factor is responsible for the transcriptional activation of the &#963;32 -like promoter of this operon, that also presents a inverted repeat called CIRCE in its regulatory region. groESL-lacZ transcription fusions with point mutations in CIRCE indicated a negative role of this cis-acting element only at normal growth temperatures, with a minor effect on heat shock induction. In addition, the expression of these fusions are no longer cell-cycle regulated, as well as GroEL synthesis in a strain which does not have the HrcA protein, the putative repressor that binds CIRCE, indicating that the CIRCE-HrcA system are involved in cell cycle regulation of groESL in C. crescentus. It was also shown that groEL is an essential gene at normal growth temperatures, since a strain with groEL disrupted is not viable. A conditional mutant was obtained by homologous recombination and in restrictive conditions growth is inhibited, DnaK levels are increased and the cells become filamentous, but no celllysis was observed. The proteins that require GroEL/GroES for proper folding have not been identified yet.
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Manakova, Elena. "Study of structure and function of GroESL chaperonin system using small angle scattering." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963646605.

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Avedissian, Marcelo. "Regulação dos genes groES e groEl em Caulobacter crescentus." Universidade de São Paulo, 1996. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-03022014-162407/.

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Os genes de choque térmico groES e groEL de Caulobacter crescentus foram isolados utilizando-se os genes homólogos de E.coli como sonda e por sequenciamento demonstrou-se que estes genes estão organizados na forma de um operon em um fragmento de DNA de aproximadamente 2,5 kb, contendo também sua região regulatória. \"Northern blots\" de RNA total de células crescidas a 300C ou submetidas a choque térmico mostraram a presença de um único RNA de tamanho aproximado de 2,3kb, altamente induzido por choque térmico, permanecendo em altos níveis mesmo após longos períodos de choque térmico. Amostras de RNA total de células sincronizadas, de diferentes estágios do ciclo celular de Caulobacter, foram também analisadas mostrando que os níveis do mRNA groESL variam durante o ciclo, apresentando um máximo na célula prédivisional. Análises através de \"Western blot\" mostraram uma pequena variação nos níveis da proteína GroEL ao longo do ciclo celular, sendo os tempos 60 e 120 minutos, respectivamente, os pontos de mínimo e máximo acúmulo da proteína concordando com os resultados obtidos em \"N orthern blots\". O mesmo tipo de análise foi feito com extratos totais obtidos a partir uma população mista de células crescidas a 300C e submetidas a choque térmico, observando-se o acúmulo da proteína até 60 minutos depois do choque térmico, com aumento da ordem de 5 vezes nos níveis de GroEL, níveis estes que diminuem lentamente a partir deste ponto. Os inícios de transcrição foram determinados em experimentos de \"primer extension\" utilizando-se RNA total de células incubadas 300C e de células submetidas a diferentes condições de choque térmico. Dois possíveis sítios de início de transcrição foram determinados nas posições -119 e -88 do ATG da metionina iniciadora de groES, sendo as regiões -10 e -35 dos promotores correspondentes (P 1 e P2) identificadas. Somente a transcrição iniciando a partir de P2, que apresenta características de um promotor transcrito pelo &#963;32, aumenta durante o choque térmico. Fusões de transcrição com o vetor repórte placZ/290 e a região 5\' regulatória do operon groESL foram construídas para identificar as sequências responsáveis pelo controle por choque térmico e pelo controle temporal. Fusões de transcrição contendo deleções na região 5\' do operon mostraram que sequências a montante do promotor P2 não são necessárias para a indução por choque térmico ou para o controle temporal. Fusões de transcrição contendo mutações sítio-dirigidas na repetição invertida, encontrada a 3\' do promotor P2, antes do gene groES, revelaram que este elemento, conhecido como CIRCE, regula negativamente a expressão de groESL a 300C e mutações neste elemento levam à perda do controle temporal deste operon.<br>The heat shock genes groES and groEL of Caulobacter crescentus were isolated using the homologous genes of E.colí as a probe. DNA sequence analysis has shown that these genes are organized as an operan in a fragment of about 2.5kb, which includes the 5\' regulatory region. Northern blot analysis of total RNA from cells grown at 300C or heat shocked treated has shown the presence of a single mRNA species for groESL, of approximately 2.3kb in size, which presented increased leveis even after long periods of heat shock. Samples of total RNA from synchronized cells, corresponding to different stages of the Caulobaaer cell cycle, were also analysed, showing that the amount of groESL mRNA varies during the cycle, with maximum leveis in predivisional cells. Western blot analysis of GroEL leveis in Caulobaaer has shown that the amount of the protein decreases during the first 60 minutes of C.crescentus cell cycle and then starts to increase again. These results corroborate the data obtained with Northern blot analysis. A similar experiment was performed after exposing a mixed population of C.crescentus cells to different times of heat shock at 400C. Western blot of extracts of these cells showed a fivefold increase in the leveis of GroEL after 60 minutes of heat shock, which then begins to decrease. Primer extension experiments were performed using total RNA from cells incubated at normal growth temperature or after heat shock treatment. Two possible transcription start sites were determined at positions -119 and -88 from the ATG of the groES initiator methionine and the -10 and -35 regions of the corresponding promoters (P 1 and P2) were identified. Only trancription initiating from the P2 promoter, which has caracteristics of a &#963;32 promoter, I ncreases during heat shock .Transcription fusions with the reporter vector placZ/290 and the 5\' regulatory region of the groESL operan were contructed in order to identify the sequences responsible for heat shock and cell cycle contral. Deletion analysis in the 5\' region of the operon showed that no sequences upstream of the P2 promoter are necessary for heat shock induction or for temporal contral. Site-directed mutagenesis in the inverted repeat found 3\' of the P2 promoter, in front of the groES gene, revealed that this element, also known as CIRCE, negatively regulates groESL expression at 300C and mutations in it lead to loss of temporal control of this heat shock operon.
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Kohler, Reto Jurg. "Studies on mechanism and applications of the GroEL/groES molecular chaperone system." Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298826.

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Kad, Neil M. "Probing the functional and conformational dynamics of the chaperonins GroEL and GroES." Thesis, University of Bristol, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265326.

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Susin, Michelle Fernanda. "Análise funcional das proteínas HrcA, GroES/GroEL e DnaK/DnaJ em Caulobacter crescentus." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-14062016-171416/.

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O operon groESL de C. crescentus apresenta dupla regulação. A indução deste operon por choque térmico é dependente do fator sigma de choque térmico &#963;32. A temperaturas fisiológicas, a expressão de groESL apresenta regulação temporal durante o ciclo celular da bactéria e o controle envolve a proteína repressora HrcA e o elemento CIRCE (controlling inverted repeat of chaperonin expression). Para estudar a atividade da proteína repressora in vitro, produzimos e purificamos de E. coli a HrcA de C. creseentus contendo uma cauda de histidinas e a ligação especifica ao elemento CIRCE foi analisada em ensaios de migração retardada em gel de poliacrilamida (EMRGP). A quantidade de DNA retardada pela ligação a HrcA aumentou significativamente na presença de GroES/GroEL, sugerindo que estas proteínas modulam a atividade de HrcA. Corroboração desta modulação foi obtida analisando fusões de transcrição da região regulatória de groESL com o gene lacZ, em células de C. crescentus produzindo diferentes quantidades de GroES/EL. HrcA contendo as substituições Pro81 AJa e Arg87Ala, aminoácidos que se localizam no domínio putativo de ligação ao DNA da proteína, mostraram ser deficientes na ligação a CIRCE, tanto in vitro como in vivo. Em adição, HrcA Ser56Ala expressa na mesma célula juntamente com a proteína selvagem produziu um fenótipo dominante-negativo, indicando que a HrcA de C. crescentus liga-se a CIRCE como um oligômero, provavelmente um dímero. As tentativas de obtenção de mutantes nulos para os genes groESL ou dnaKJ falharam, indicando que as proteínas GroES/GroEL e DnaK/DnaJ são essenciais em C. crescentus, mesmo a temperaturas normais. Foram então construídas no laboratório as linhagens mutantes condicionais SG300 e SG400 de C. crescentus, onde a expressão de groESL e de dnaKJ, respectivamente, está sob controle de um promotor induzido por xilose (PxyIX). Estas linhagens foram caracterizadas quanto á sua morfologia em condições permissivas ou restritivas, assim como quanto à capacidade de sobrevivência frente a vários tipos de estresse. As células da linhagem SG300, exauridas de GroES/GroEL, são resistentes ao choque térmico a 42°C e são capazes de adquirir alguma termotolerância. Entretanto, estas células são sensíveis aos estresses oxidativo, salino e osmótico. As células da linhagem SG400, exauridas de DnaKlJ, são sensíveis ao choque térmico, à exposição a etanol e ao congelamento, e são incapazes de adquirir termotolerância. Além disso, tanto as células exauridas de GroES/GroEL quanto as exauridas de DnaK/DnaJ apresentam problemas na sua morfologia. As células de SG300 exauridas de GroES/GroEL formam filamentos longos que possuem constrições fundas e irregulares. As células de SG400 exauridas de DnaK/DnaJ são apenas um pouco mais alongadas que as células pré-divisionais selvagens e a maioria das células não possuem septo. Estas observações indicam bloqueio da divisão celular, que deve ocorrer em diferentes estágios em cada linhagem.<br>In Caulobacter crescentus, the groESL operon presents a dual type of control. Heat shock induction of the operon is dependent on the heat shock sigma factor &#963;-32. At physiological temperatures, groESL expression is cell cycle regulated and the control involves the repressor protein HrcA and the element CIRCE (controlling inverted repeat of chaperonin ~xpression). To study the activity of HrcA in vitro, we produced and purified from E. coli a histidine-tagged version of the protein, and specific binding to the CIRCE element was analyzed in electrophoretic mobility shift assays (EMSA). The amount of retarded DNA increased significantly in the presence of GroES/GroEL, suggesting that these proteins modulate HrcA activity. Further evidence of this modulation was obtained using lacZ transcription fusions with the groESL regulatory region in C. crescentus cells producing different amounts of GroES/GroEL. The mutants proteins HrcA Pro81Ala and HrcA Arg87Ala, that contain amino acid substitutions in the putative DNA-bindíng domain of the protein, were found to be deficient in binding to CIRCE in vitro and in vivo. Furthermore, HrcA Ser56Ala expressed together with the wild type protein within the same cell, produced a dominant-negative phenotype, indicating that C. crescentus HrcA binds to CIRCE in an oligomeric form, most likely as a dimer. Attempts to obtain null mutants for groESL or dnaKJ were unsuccessful indicating the importance of GroES/GroEL and DnaK/lDnaJ to the survival of C. crescentus cells. Conditional mutants were then constructed in our laboratory in which groESL and dnaKJ expression is under the control ofaxylose inducible promoter (PxyIX) , giving rise to strains SG300 and SG400, respectively. These strains were characterized in regard to their morphology under permissive and restrictive conditions, as well as their viability under different types of environmental stresses. SG300 cells depleted of GroES/GroEL are resistant to heat shock at 42°C and can acquire some thermotolerance, but they are sensitive to oxidative, saline and osmotic stresses. SG400 cells depleted of DnaKlJ are quite sensitive to heat shock, ethanol and freezing, and are unable of acquiring thermotolerance. Cells depleted of either GroES/EL or DnaKlJ also present morphological problems. SG300 cells depleted of GroES/EL form long and pinched filaments. SG400 cells depleted of DnaKlJ are only somewhat more elongated than wild-type predivisional cells and most cells do not present septum. These observations indicate a cell division arrest, which should occur at different stages in each strain.
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Tang, Yun-Chi. "Structural Features of the GroEL-GroES Nano-Cage Required for Rapid Folding of Encapsulated Protein." Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-73047.

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DE, CROUY CHANEL AXELLE. "Interaction entre les machines chaperons dnak/dnaj/grpe et groel/groes et leurs proteines substrats." Paris 7, 1997. http://www.theses.fr/1997PA077103.

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Les molecules chaperons forment une classe de proteine qui fixent selectivement les polypeptides naissants, non replies, mal replies, ou agreges, en reconnaissant des regions hydrophobes exposees par les proteines depliees. Cette propriete est la base de l'implication des machines chaperons (dnak/dnaj/grpe) et (groel/groes) dans des processus cellulaires tels que le repliement, l'adressage, la renaturation des proteines, et le controle des interactions proteine-proteine. Les chaperons fonctionnent en collaboration avec leur cochaperon. Nous avons etudie l'interaction entre les machines chaperons d'e. Coli et leurs substrats proteiques et l'effet produit par la presence des cochaperons. Groes diminue la specificite de groel pour les acides amines hydrophobes et augmente celle pour les acides amines hydrophiles. Dnaj attenue les sites hydrophobes de dnak cependant qu'il renforce le site arg/lys. Par ailleurs, dnaj augmente significativement l'interaction entre dnak et les peptides en helice. Au contraire des hsp90 qui semblent interagir avec de nombreuses proteines natives, dnak et groel interagissent principalement avec des proteines depliees. Cependant, nous avons montre que dnak et groel interagissent plus frequemment qu'on ne le suppose avec les proteines natives. L'affinite de dnak est en correlation avec l'hydrophobicite de surface des proteines natives. Nous avons etudie l'interaction entre dnak et les proteines membranaires, et nous avons montre que dnak interagit fortement avec les proteines membranaires solubilisees et beaucoup moins avec celles inserees dans les membranes. Enfin, dans une etude in vitro, nous avons montre que dnaj presente une activite proteine disulfure isomerase. Et nous presentons en plus, un travail portant sur l'expression et l'etat d'oxydoreduction des proteines d'e. Coli dans des mutants redox et notamment des mutants de dnaj.
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Castanié-Cornet, Marie-Pierre. "Etude fonctionnelle et rôle physiologique des protéines chaperons GroES et GroEL de la bactérie Eschericchia coli." Toulouse 3, 1997. http://www.theses.fr/1997TOU30262.

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Les chaperons moleculaires forment une famille de proteines dont le role est d'aider d'autres polypeptides a se replier, sans pour autant intervenir dans leur structure finale et dans leur fonction. Chez e. Coli, la chaperonine groel, 60kda, fonctionne avec son cochaperon, groes pour promouvoir via l'hydolyse d'atp, le repliement de substrats partiellement denatures ; ces deux proteines sont necessaires a la vie cellulaire a toutes temperatures. Nous avons realise une etude structurale et fonctionnelle d'une proteine groel tronquee pour 141 acides amines dans sa partie carboxy-terminale. Cette proteine est apparue capable, d'une part de former des dimeres in vivo et in vitro, et d'autre part, d'interagir avec groes de maniere beaucoup plus forte que la proteine sauvage. Elle ne possede cependant plus d'activite atpase mais peut encore fixer des substrats denatures, et ce malgre l'absence de cavite centrale. Dans un deuxieme temps, nous nous sommes interesses a la place des proteines groe dans la physiologie cellulaire, ainsi qu'a l'identification de leurs cibles. Nous avons construit une souche deletee pour l'operon groe, et possedant ces genes sur un plasmide sous controle d'un promoteur inductible. En situation de carence en proteines groe, divers phenotypes ont ete observes, similaires a ceux causes par une mutation groe thermosensible. Cette souche ne nous a pas permis d'isoler des suppresseurs multicopies pouvant pallier a la carence en proteines groe. Une autre approche, basee sur la suppression du phenotype thermosensible de mutants groe, a donc ete utilisee pour rechercher des cibles critiques ou des partenaires des proteines groe. De cette maniere, nous avons identifie un suppresseur multicopie de la thermosensibilite associee a une mutation groe : le gene rof ; il code pour une petite proteine de fonction inconnue, qui copurifie avec la proteine rho, un facteur de terminaison de la transcription. Le mecanisme de la suppression mediee par rof est actuellement en cours d'investigation. De plus, nous avons entrepris une recherche de suppresseurs chromosomiques de la thermosensibilite liee a des mutations groe. Pour l'instant, nous avons identifie les regions du chromosomes ou se situent ces mutations suppressives et l'identification precise des loci impliques devrait permettre la mise en evidence de certaines cibles critiques, ou de partenaires, des chaperons groe.
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Sbai, Meryem. "Intervention des chaperonines DNAK/DNAJ et des Chaperonines GROES/GROEL dans la biogenèse des ribosomes d'Escherichia Coli." Paris 7, 1999. http://www.theses.fr/1999PA07GA05.

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Books on the topic "GroESL"

1

Y groes naidd. Gomer, 2012.

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Grosse, John. Great Grosse blessings: Grosse segen. J. Grosse, 2008.

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Henkel & Grosse: 100 Jahre Leidenschaft für/100 years of passion for Grossé + Bijoux Christian Dior. Arnoldsche Art Publishers, 2010.

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Grouse and grouse hunting. Nick Lyons Books, 1987.

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Steen, Johan Buch. Ryper: Rypeliv og rypejakt. Gyldendal, 1994.

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Bojinović, Miloš. Gromil polje. Knj. zadruga, 2003.

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Schreiber, Wolfgang. Grosse Dirigenten. Piper, 2005.

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Sharpe, Tom. The Gropes. Hutchinson, 2009.

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Koncsik, Imre. Grosse Vereinheitlichung? Verlag Dr. Kovač, 2000.

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Grosse Passivhäuser. W. Kohlhammer, 2004.

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Book chapters on the topic "GroESL"

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Ishii, Noriyuki. "GroEL and the GroEL-GroES Complex." In Subcellular Biochemistry. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-46503-6_17.

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Liu, Han, and Peter A. Lund. "The Roles of GroES as a Co-Chaperone for GroEL." In Networking of Chaperones by Co-Chaperones. Springer New York, 2007. http://dx.doi.org/10.1007/978-0-387-49310-7_7.

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Luyten, E., K. M. Vlassak, C. Verreth, and J. Vanderleyden. "Characterization of a GroESL Homologue in Rhizobium sp. BR816: A Possible Role in the Host Range Extension of Various Rhizobium Strains to Leucaena leucocephala." In Biological Nitrogen Fixation for the 21st Century. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5159-7_118.

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Rosen, Elisheva. "Grotesk." In Ästhetische Grundbegriffe. J.B. Metzler, 2001. http://dx.doi.org/10.1007/978-3-476-00517-5_29.

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Rosen, Elisheva, Jörg W. Rademacher, and Maria Kopp. "Grotesk." In Ästhetische Grundbegriffe. J.B. Metzler, 2010. http://dx.doi.org/10.1007/978-3-476-00521-2_29.

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Baneyx, François, and Anthony A. Gatenby. "GroEL-Mediated Protein Folding." In Protein Folding. American Chemical Society, 1993. http://dx.doi.org/10.1021/bk-1993-0526.ch010.

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Weiss, Peter A. M. "Großes Kind." In Sectio Caesarea und assoziierte Fragen. Springer Vienna, 1994. http://dx.doi.org/10.1007/978-3-7091-6633-8_14.

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Baum, H. "Blutbild, großes." In Springer Reference Medizin. Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_587.

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Baum, H. "Blutbild, großes." In Lexikon der Medizinischen Laboratoriumsdiagnostik. Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_587-1.

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Peck, Stewart B., Carol C. Mapes, Netta Dorchin, et al. "Grouse Locusts." In Encyclopedia of Entomology. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_1208.

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Conference papers on the topic "GroESL"

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Пичкур, Е. Б., С. С. Кудрявцева, А. В. Моисеенко, И. А. Ярошевич та Т. Б. Станишнева-Коновалова. "Структура асимметричного GroEL-GroES комплекса по данным криоэлектронной микроскопии". У XXVIII Российская конференция по электронной микроскопии и VI школа молодых учёных "Современные методы электронной, зондовой микроскопии и комплементарные методы в исследованиях наноструктур и наноматериалов". Crossref, 2020. http://dx.doi.org/10.37795/rcem.2020.78.77.006.

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Noschinski, L., E. Bensmann, and M. Braun. "Großes axilläres Lipom." In Wissenschaftliche Abstracts zur 40. Jahrestagung der Deutschen Gesellschaft für Senologie e.V. (DGS) Interdisziplinär. Kommunikativ. Digital. Georg Thieme Verlag KG, 2021. http://dx.doi.org/10.1055/s-0041-1730140.

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Wetzel, Markus, and Francisco Vazquez. "Stadthöfe Hamburg – Innovative Structural Design between Water, Heritage and Modern Lifestyle." In IABSE Congress, New York, New York 2019: The Evolving Metropolis. International Association for Bridge and Structural Engineering (IABSE), 2019. http://dx.doi.org/10.2749/newyork.2019.2530.

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&lt;p&gt;Along the Stadthausbrücke between the roads of Neuer Wall and Grosse Bleichen, behind historic facades, a new quarter with a gross floor space of approx. 45.000 m² has been put under construction for retail, office and residential use. The high complexity of the planning and construction task results from the many constraints caused by the tight inner-city situation. In particular, the small scale and nested courtyard-like arrangement of the objects, the boundary development and development of the Alsterfleet, a former waterbody, as well as the demanding requirements of heritage protection were especially challenging.&lt;/p&gt;
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Paes Leme, Renato, and Inbal Talgam-Cohen. "Gross Substitutes." In EC '18: ACM Conference on Economics and Computation. ACM, 2018. http://dx.doi.org/10.1145/3219166.3277558.

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Dias, Thyago, Robson De Melo, and Nivaldi Calonego Junior. "Análise do Algoritmo Round-Robin para balanceamento de cargas em Redes Definidas por Software." In Escola Regional de Informática de Mato Grosso. Sociedade Brasileira de Computação - SBC, 2019. http://dx.doi.org/10.5753/eri-mt.2019.8585.

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O algoritmo Round-Robin é amplamente usado para o escalonamento de processo, podendo ser aplicado para o escalonamento de requisições em redes SDN. Este artigo discute os resultado obtidos por meio de simulação do balanceamento de carga em redes SDN, usando o algoritmo Round-Robin. Os resultados obtidos mostram a eficácia do balanceamento de carga no ambiente SDN. No cenário em que existiu congestionamento de tráfego na rede o ambiente SDN com balanceamento de carga atuou 109% mais rápido em comparação ao ambiente não SDN sem balanceamento de carga.
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Melo, Thadeu, and Karin Komati. "Análise Exploratória de Dados Industriais da Fabricação de Placas de Média Densidade." In Escola Regional de Informática de Mato Grosso. Sociedade Brasileira de Computação - SBC, 2019. http://dx.doi.org/10.5753/eri-mt.2019.8586.

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Este artigo descreve a análise exploratória de dados do processo de fabricação de placas MDF de uma empresa no estado do Espírito Santo. O objetivo é avaliar quais dos atributos do processo estão correlacionados com a propriedade de resistência à tração perpendicular, que é um dos requisitos da norma ABNT 15316-2. Após analisar dados de quatro meses de produção, verificou-se que a preocupação é com a produção de duas espessuras de placas, para 9mm e 25mm. O método de correlação de Pearson, indicou um subconjunto de cinco atributos que mais afetam o resultado, dentre as 23 iniciais: dosagem de resina, energia, velocidade e fator de prensagem e umidade da fibra. Diferente do que outros trabalhos indicam, nem sempre a alta dosagem de resina garante a qualidade do produto final, pelo menos para a placa de 9mm.
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Martins, Ernane, and Luís Manuel Gouveia. "Aprendizagem Móvel na Produção Científica Indexada ao Scopus nos Anos de 2016 e 2017." In Escola Regional de Informática de Mato Grosso. Sociedade Brasileira de Computação - SBC, 2019. http://dx.doi.org/10.5753/eri-mt.2019.8587.

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O objetivo deste trabalho é conhecer os estudos sobre aprendizagem móvel realizados nos últimos anos indexados a base de dados Scopus. Para alcançar este objetivo buscou-se, através do método da revisão sistemática da literatura, traçar um panorama sobre a produção científica relacionada a este tema nos anos de 2016 e 2017, com a finalidade de permitir a visualização de possíveis lacunas a serem aprofundadas, possibilitando novas oportunidades de pesquisa. Como resultados são apresentadas as características das publicações selecionadas dentro do escopo determinado.
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Dos Santos, Suzani, Robson De Melo, and Nivaldi Calonego Junior. "Arcabouço de Vigilância Inteligente para Operações de Compras Eletrônicas no Contexto de Smart Cities." In Escola Regional de Informática de Mato Grosso. Sociedade Brasileira de Computação - SBC, 2019. http://dx.doi.org/10.5753/eri-mt.2019.8588.

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Intelligent surveillance systems aim to identify suspicious activity beyond the observation of people and environments, as computer networks and computer systems remain vulnerable to malicious actions. The intelligent surveillance framework (Arcabouço de Vigilância Inteligente - AVI) uses the cognitive construction of a user profile and the recognition of devices for electronic purchases, aiming to ensure the authenticity of transactions for computer systems in networked interconnected. The AVI assessment shows that it is effective with respect to user authenticity and that the smart surveillance model in purchasing operations matches the expectation of vulnerability reduction.
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Geraldes, Wendell Bento, Ernane Rosa Martins, and Ulisses Rodrigues Afonseca. "Avaliação da Usabilidade do Scratch utilizando o Método System Usability Scale (SUS)." In Escola Regional de Informática de Mato Grosso. Sociedade Brasileira de Computação - SBC, 2019. http://dx.doi.org/10.5753/eri-mt.2019.8589.

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A linguagem visual de programação Scratch foi criada em 2007 no MIT, tornando-se largamente utilizada no mundo inteiro. Este artigo tem como objetivo avaliar a usabilidade da linguagem em sua versão on-line disponível na internet. Para esta finalidade foi utilizado o método System Usability Scale (SUS), criado por John Brooke em 1986, que é considerado um dos questionários mais confiáveis e válidos para medir a usabilidade rercebida pelos usuários. A coleta de dados foi realizada através de um questionário on-line contendo 10 perguntas, com uma escala de 1 a 5, onde 1 significa Discordo totalmente e 5 Concordo totalmente. Os resultados obtidos demostraram uma avaliação positiva dos usuários em relação a linguagem Scratch.
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Silva, Matheus, and Thiago Ventura. "Classificação Morfológica de Galáxias Por Meio de Redes Neurais." In Escola Regional de Informática de Mato Grosso. Sociedade Brasileira de Computação - SBC, 2019. http://dx.doi.org/10.5753/eri-mt.2019.8590.

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This paper proposes the development of a convolutional neural network for the morphological classification of galaxies through optical images, classifying them into six distinct classes based on the Hubble Tuning Fork model. In order to automate the mass identification and separation of the huge volume of data generated in recent astronomical observatories, deep learning and data augmentation techniques are used to generate increased data variation and consequently improve network accuracy. Our model achieved an average precision of 88%.
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Reports on the topic "GroESL"

1

Bos, Ernst, and Theo Vogelzang. Groei versus groen : drie casestudy’s over de waarde van het stadsgroen in Amsterdam. Wageningen University & Research, Wetenschapswinkel, 2018. http://dx.doi.org/10.18174/443008.

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Dijkshoorn-Dekker, M. W. C., K. Soma, and A. T. Blaeij. Groene initiatieven in de stad : handelingsperspectief provincies voor het stimuleren van maatschappelijke betrokkenheid bij groen in de stad. Wageningen Economic Research, 2017. http://dx.doi.org/10.18174/424282.

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Davis, Steve, and John Haltiwanger. Gross Job Creation, Gross Job Destruction and Employment Reallocation. National Bureau of Economic Research, 1991. http://dx.doi.org/10.3386/w3728.

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Vijn, Marcel, and Marien Borgstein. De rol van Vereniging Buitenstad in het Almeerse groen : hoe kunnen burgers en groene organisaties samen de kwaliteit en de identiteit van het stadslandschap versterken? Wageningen University & Research, Wetenschapswinkel, 2016. http://dx.doi.org/10.18174/394446.

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Jursca, J., R. Draper, R. Bibby, and D. Wruck. White Paper on Soil Prep for Gross Alpha and Gross Beta. Office of Scientific and Technical Information (OSTI), 2021. http://dx.doi.org/10.2172/1759977.

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John Reisenauer. Sage-Grouse Lek Guideline Review Report. Office of Scientific and Technical Information (OSTI), 2011. http://dx.doi.org/10.2172/1023472.

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Berger, Matthew. Sharp-tailed Grouse Restoration; Colville Tribes Restore Habitat for Sharp-tailed Grouse, Annual Report 2001-2002. Office of Scientific and Technical Information (OSTI), 2003. http://dx.doi.org/10.2172/963046.

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Whitney, Richard. Sharp-tailed Grouse Restoration; Colville Tribes Restore Habitat for Sharp-tailed Grouse, Annual Report 2002-2003. Office of Scientific and Technical Information (OSTI), 2004. http://dx.doi.org/10.2172/963066.

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van Dam, Jan, Paulien Harmsen, Harriëtte Bos, and Richard Gosselink. Lignine : groene grondstof voor chemicaliën en materialen. Wageningen Food & Biobased Research, 2016. http://dx.doi.org/10.18174/398437.

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Hendriks, Kees, and Saskia Hommes. Groene daken in Tilburg : ervaringen, motieven en opschalingsmogelijkheden. Wageningen Environmental Research, 2016. http://dx.doi.org/10.18174/400304.

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