Academic literature on the topic 'Guinea pigs Anatomy'

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Journal articles on the topic "Guinea pigs Anatomy"

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Goksu, Nebil, Nalan Karademir, Rifki Haziroglu, Ismet Bayramoglu, Yusuf Kemaloglu, and Necmettin Akyeldiz. "Anatomy of the Guinea Pig Temporal Bone." Annals of Otology, Rhinology & Laryngology 101, no. 8 (August 1992): 699–704. http://dx.doi.org/10.1177/000348949210100814.

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The middle ear of guinea pigs has long been used for experimental studies, but no detailed information about its temporal bone anatomy is available. In 18 adult guinea pigs, the temporal bone, eustachian tube, and inner ear anatomy, in addition to the anatomy of the middle ear, were investigated under the dissection microscope. In addition to properties of the eardrum, ossicles, air cell system, and cochlea previously described, the appearance of Huschke's foramen and the crista stapedis in an adult guinea pig ear, the structure of the eustachian tube, the architecture of the internal auditory canal, and the communication of the mastoid cells with the tympanic bulla are described. Differences and similarities among guinea pigs, other experimental animals, and humans are discussed to show the advantages and disadvantages of the guinea pig ear for experimentation.
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Aharinejad, S., P. Franz, H. Sinzinger, and W. Firbas. "Esophageal Prostaglandins in Guinea Pigs and Rats." Cells Tissues Organs 139, no. 1 (1990): 66–69. http://dx.doi.org/10.1159/000146980.

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Yarto-Jaramillo, Enrique. "Respiratory System Anatomy, Physiology, and Disease: Guinea Pigs and Chinchillas." Veterinary Clinics of North America: Exotic Animal Practice 14, no. 2 (May 2011): 339–55. http://dx.doi.org/10.1016/j.cvex.2011.03.008.

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Legendre, Loic. "Anatomy and Disorders of the Oral Cavity of Guinea Pigs." Veterinary Clinics of North America: Exotic Animal Practice 19, no. 3 (September 2016): 825–42. http://dx.doi.org/10.1016/j.cvex.2016.04.006.

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Maxeiner, Stephan, Selina Gebhardt, Frederick Schweizer, Agnes E. Venghaus, and Gabriela Krasteva-Christ. "Of mice and men – and guinea pigs?" Annals of Anatomy - Anatomischer Anzeiger 238 (November 2021): 151765. http://dx.doi.org/10.1016/j.aanat.2021.151765.

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Alp, H., B. Durgun, S. Müftüoğlu, S. Inan, and E. Aşan. "Ultrastructural Changes of Epidermal Langerhans Cells in Pregnant Guinea Pigs." Cells Tissues Organs 149, no. 2 (1994): 100–103. http://dx.doi.org/10.1159/000147563.

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de Faria, Marcelo, Adriana Gradela, Ana Santos, Ítalo Lopes, Vanessa Inoue, and Bárbara Brito. "Participation of the Intercostal Nerves to the Innervation of the Diaphragm Muscle in Cavia porcellus." Journal of Morphological Sciences 36, no. 01 (March 2019): 024–27. http://dx.doi.org/10.1055/s-0039-1683406.

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Introduction The diaphragm is the leading respiratory muscle. It is innervated mainly by the diaphragmatic nerve, and, in some species, by the delicate fibers of the intercostal nerves. In guinea pigs, there is no description of these anatomic structures that innerve this important muscle. This study aimed to analyze the participation of the intercostal nerves to the innervations of the diaphragm of guinea pigs of both sexes. Materials and Methods We studied 40 guinea pigs (Cavia porcellus) of both sexes. We fixed and dissected the diaphragm of the specimens used in the experiment to assess the path of the intercostals nerves in both the body antimeres. Results The diaphragm was innervated by the intercostal nerve pairs 6 through 12, and, less frequently, by the 8th nerve (38/40 = 95%), followed by the 7th (36/40 = 90%) and subsequently by the 9th (32/40 = 80%). The 12th nerve presented the lowest frequency (2/20 = 10%) in both genders. All nerve pairs displayed similar occurrence compared with the gender and the antimeric disposition. The only exception was the 9th nerve, which presented a significant variation of the occurrence, both in relation to gender and antimeric disposition. From a statistic point of view, all nerves were independent. We observed no correlation between the gender and their position. Conclusions We shall conclude that the diaphragm of guinea pigs is innervated by the 6th through 12th pairs of intercostal nerves, with the 7th, 8th, and 9th being the primary providers. There is no interference of the variables gender or antimeric disposition on the behavior of the intercostal nerves of guinea pigs as refers to their origin and participation to the innervations of the diaphragm.
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Lohse, C. L., K. L. Cox, A. T. W. Cheung, and J. A. Negulesco. "Effects of Growth and Maturation on Biliary Structures of Guinea Pigs." Cells Tissues Organs 128, no. 3 (1987): 177–83. http://dx.doi.org/10.1159/000146336.

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Ghiz, Adam F., Alec N. Salt, John E. DeMott, Miriam M. Henson, O. William Henson, and Sally L. Gewalt. "Quantitative anatomy of the round window and cochlear aqueduct in guinea pigs." Hearing Research 162, no. 1-2 (December 2001): 105–12. http://dx.doi.org/10.1016/s0378-5955(01)00375-6.

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Zeng, L., M. Takeya, X. Ling, A. Nagasaki, and K. Takahashi. "Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species." Journal of Histochemistry & Cytochemistry 44, no. 8 (August 1996): 845–53. http://dx.doi.org/10.1177/44.8.8756757.

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We examined interspecies reactivities of eight anti-human monocyte/macrophage monoclonal antibodies (MAbs), Am-3K, PM-2K, X4, X14, Ber-MAC3, GHI/61, EBM/11, and KP1, with various animal tissues including rats, guinea pigs, rabbits, cats, dogs, goats, pigs, bovines, horses, and monkeys. All MAbs recognized monkey macrophages. Pig macrophages were detected by most MAbs except for EBM/11 and KP1. Of the eight antibodies, AM-3K showed the widest interspecies reactivity. It reacted with macrophages of all animal species examined, except for rats. Western blot analysis revealed a similarity in the antigens recognized by AM-3K among guinea pigs, rabbits, and humans. Other anti-human MAbs demonstrated distinct reactive patterns against macrophages in animals. The immunostaining patterns of all of these MAbs in animal tissues were similar to those found in humans, although some MAbs, such as AM-3K, EBM/11, and X4, displayed more restricted reactivity in animals than in humans. These results indicate that some anti-human monocyte/macrophage MAbs are also available for immunohistochemical detection of monocyte/macrophages in animal tissues. Among them, AM-3K is considered to be the most useful MAb for identifying macrophages in various tissues of animals.
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Dissertations / Theses on the topic "Guinea pigs Anatomy"

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Kardegar, Nadia. "Electrical Brain Stimulation and Depressive-like Behavior in Guinea Pigs." Wright State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=wright1342408797.

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Coussons, Peter John. "An investigation of the specificity of guinea pig liver transglutaminase towards protein substrates." Thesis, University of Stirling, 1991. http://hdl.handle.net/1893/21845.

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The specificity of guinea pig liver transglutamlnase was Investigated by the determination of modification sites within polypeptides and proteins of known sequence and (In some cases) folded structure. It was shown that some globular proteins have substrate properties for transglutamlnase In conformations which resemble their native states. Novel substrate sites for transglutamlnase were determined within the following proteins: 1) bovine B-Iactoglobulln. 2) the Hls3~ Gln388 mutant form of yeast phosphoglycerate kinase. 3) bovine B-caseln. 4) porcine pepsin. Despite the high exposure of many glutamlnyl residues within these proteins only a small fraction of these residues were observed to be reactive towards transglutamlnase. This Is taken to Indicate that features such as the chemical nature of the amino acid side chains In the local vicinity of unreactive glutamlnyl residues strongly determine the specificity of transglutamlnase. When structural models were available for substrates of transglutamlnase, the local secondary structure associated with substrate sites could be assessed. When no such models were available computer based methods were used to predict the local secondary structures associated with these sites. This approach allows substrate sites to be classified according to their local conformational preference Into conformationally flexl ble (type A substrates) and more conformationally restricted (type B substrates). Since diverse amino acid sequences are observed to surround the reactive glutamlnyl residues of many of the non-physiological substrates of transglutamlnase, It was assumed that the glutamlnyl residues within these sequences were probably reactive due to having; 1) a favourable stereochemistry during modification by transglutaminase 2) a lack of Inhibitory features. In order to determine why some exposed glutamlnyl residues were reactive and others not, It was necessary to find features which were present In unreactive sequences but absent In reactive ones. Through the use of this approach an "anti-consensus sequence" motif was Identified. This was based on the observation that exposed glutamlnyl residues which were unreactive towards transglutamlnase often have charged residues within their surrounding sequences. The distribution of allowed/disallowed residues within substrate sequences, together with what Is known concerning the conformational preference of transglutamlnase for its substrates was built Into a preliminary set of "rules'·. These rules may provide a basis for understanding the observed specificity of transglutamlnase. The application of these rules to a number of model systems has resulted In the correct prediction of both reactive and unreactive glutamlnyl side chains within a number of proteins. The demonstration of the substrate properties of the Hls3~ln388 mutant of phosphoglycerate kinase Illustrates the feasibility of Introducing a novel substrate site for transglutamlnase Into a protein using recombinant DNA technology.
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Whitmer, Deborah Lou. "Spatial and temporal characterizatioin of intercellular calcium waves in longitudinal smooth muscle of guinea pig ileum and distal colon /." abstract and full text PDF (free order & download UNR users only), 2005. http://0-wwwlib.umi.com.innopac.library.unr.edu/dissertations/fullcit/3209122.

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Thesis (Ph. D.)--University of Nevada, Reno, 2005.
"December, 2005." Includes bibliographical references (leaves 194-212). Online version available on the World Wide Web. Library also has microfilm. Ann Arbor, Mich. : ProQuest Information and Learning Company, [2005]. 1 microfilm reel ; 35 mm.
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譚銓株 and Chuen-chu Tam. "Hormonal effects of the lateral prostate and seminal vesicle of the guinea pig: an ultrastructural, morphometricand cytochemical study." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1989. http://hub.hku.hk/bib/B3123169X.

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Mazzuoli, Gemma <1979&gt. "Mechanosensitivity in the myenteric plexus of the guinea pig ileum." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2009. http://amsdottorato.unibo.it/2029/.

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The enteric nervous system regulates autonomously from the central nervous system all the reflex pathways that control blood flow, motility, water and electrolyte transport and acid secretion. The ability of the gut to function in isolation is one of the most intriguing phenomenons in neurogastroenterology. This requires coding of sensory stimuli by cells in the gut wall. Enteric neurons are prominent candidates to relay mechanosensitivity. Surprisingly, the identity of mechanosensitive neurons in the enteric nervous system as well as the appropriate stimulus modality is unknown despite the evidence that enteric neurons respond to sustained distension. Objectives: The aim of our study was to record from mechanosensitive neurons using physiological stimulus modalities. Identification of sensory neurons is of central importance to understand sensory transmission under normal conditions and in gut diseases associated with sensorimotor dysfunctions, such as Irritable Bowel Syndrome. Only then it will be possible to identify novel targets that help to normalise sensory functions. Methods: We used guinea-pig ileum myenteric plexus preparations and recorded responses of all neurons in a given ganglion with a fast neuroimaging technique based on voltage sensitive dyes. To evoke a mechanical response we used two different kinds of stimuli: firstly we applied a local mechanical distortion of the ganglion surface with von Frey hair. Secondarily we mimic the ganglia deformation during physiological movements of myenteric ganglia in a freely contracting ileal preparation. We were able to reliably and reproducibly mimic this distortion by intraganglionic injections of small volumes of oxygenated and buffered Krebs solution using stimulus parameters that correspond to single contractions. We also performed in every ganglion tested, electrical stimulations to evoke fast excitatory postsynaptic potentials. Immunohistochemistry reactions were done with antibodies against Calbindin and NeuN, considered markers for sensory neurons. Results: Recordings were performed in 46 ganglia from 31 guinea pigs. In every ganglion tested we found from 1 to 21 (from 3% to 62%) responding cells with a median value of 7 (24% of the total number of neurons). The response consisted of an almost instantaneous spike discharge that showed adaptation. The median value of the action potential frequency in the responding neurons was 2.0 Hz, with a recording time of 1255 ms. The spike discharge lasted for 302 ± 231 ms and occurred only during the initial deformation phase. During sustained deformation no spike discharge was observed. The response was reproducible and was a direct activation of the enteric neurons since it remained after synaptic blockade with hexamethonium or ω-conotoxin and after long time perfusion with capsaicin. Muscle tone appears not to be required for activation of mechanosensory neurons. Mechanosensory neurons showed a response to mechanical stimulation related to the stimulus strength. All mechanosensory neurons received fast synaptic inputs. There was no correlation between mechanosensitivity and Calbindin-IR and NeuN-IR (44% of mechanosensitive neurones Calb-IR-/NeuN-IR-). Conclusions: We identified mechanosensitive neurons in the myenteric plexus of the guinea pig ileum which responded to brief deformation. These cells appear to be rapidly accommodating neurons which respond to dynamic change. All mechanosensitive neurons received fast synaptic input suggesting that their activity can be highly modulated by other neurons and hence there is a low stimulus fidelity which allows adjusting the gain in a sensory network. Mechanosensitivity appears to be a common feature of many enteric neurons belonging to different functional classes. This supports the existence of multifunctional enteric neurons which may fulfil sensory, integrative and motor functions.
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Vasconcelos, Carlos Augusto Carvalho de. "Aspectos descritivos e quantitativos da anatomia macroscópica e microscópica do nervo vestíbulo-coclear de cobaias." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/17/17140/tde-28092006-091016/.

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O nervo vestíbulo-coclear da cobaia possui peculiaridades não encontradas em outros nervos periféricos. Não foram encontradas informações detalhadas sobre os aspectos morfométricos do VIII nervo craniano em cobaias adultas na literatura. A avaliação descritiva e quantitativa no presente estudo, evidencia informações que precedem o estudo das alterações que ocorrem em modelos experimentais de neuropatias do VIII nervo craniano e as doenças ou lesões que possam afetar o homem. Foram utilizadas 8 cobaias adultas, perfundidas com glutaraldeído a 2,5% em tampão cacodilato de sódio (0,025 M). Seus nervos direito e esquerdo (n=6, 4 nervos direito e 2 esquerdos) foram dissecados na região mediana do nervo e pós fixado com tetróxido de ósmio (OsO4) a 1% e incluídos em resina epóxi Poly/Bed 812®. Os fragmentos foram cortados em secções transversais semifinas seriadas (6 nervos) com uma espessura de 0,5 μm e corados com azul de toluidina para a microscopia de luz. Foram analisados os aspectos histológicos descritivos e topográficos do nervo vestíbulo-coclear em cobaias adultas, bem como os aspectos histométricos na parte mediana do nervo, no referente a densidade de fibras mielínicas, a distribuição dos diâmetros de tal tipo de fibras mielínicas, ao cálculo da razão G e o número de vasos sangüíneos encontrados dentro da área de cada nervo. O nervo coclear é envolvido pelo vestibular na porção inicial da junção de ambos. O conjunto, nervos coclear e vestibular e nervo vestíbulo-coclear têm a aparência bifurcada da letra Y, na horizontal com os ramos lateralizados, possuindo em média 5 mm de comprimento. É ricamente vascularizado e documenta uma completa interposição das fibras dos nervos coclear e vestibular, quando o fascículo do VIII nervo está completamente constituído. As suas fibras mielínicas dispõem-se paralelamente em seu sentido longitudinal e são circundadas por escasso tecido endoneural. Não foram evidencias fibras amielínicas no VIII nervo. A distribuição das fibras mielínicas no segmento mediano do VIII nervo craniano foi unimodal, havendo predomínio de fibras com 3,5 m de diâmetro. O número de fibras foi de 5.390 ± 1.504 fibras. O diâmetro das fibras variou de 1,5 a 6 m de diâmetro. Os axônios com 2,5 m de diâmetro foram predominantes quantitativamente: 6.757 ± 1.922 axônios. O diâmetro axonal variou entre 1 a 4,5 m. A densidade média das fibras do VIII nervo em seu segmento mediano foi de 41.474 ± 4384 fibras/mm2. O diâmetro fascicular foi de 0,77 ± 0,04 mm. A razão G varia de 0,2 a 0,9. O maior número de fibras tem razão G de 0,7 e 0,6, respectivamente, predominando as fibras com 0,7. Os resultados descritos neste trabalho científico são originais, contendo alguns resultados inéditos e pouco estudados na literatura científica sobre a anatomia e morfometria do VIII nervo craniano em cobaia. O nervo vestíbulo-coclear da cobaia é um nervo sensitivo aferente especial, e bem diferente em sua anátomo-fisiologia de todos os outros nervos sensitivos e motores encontrados nos mamíferos. A cobaia adulta é um excelente modelo experimental para o estudo do nervo vestíbulo-coclear, semelhantes aos de humanos.
The vestibulocochlear (VIIIth cranial nerve) nerve of guinea pigs have special features not common for peripheral nerves in general. There are no detailed reports on the morphometric characteristics of the VIII nerve in adult guinea pigs. This is a descriptive and qualitative study that shows normal parameters that are useful in experimental models of the VIIIth nerve neuropathy. Eight adult guinea pigs were perfused with 2.5% glutaraldehyde in isotonic cacodilate buffer (0.025M). The right (N=4) and left (N=2) nerves were dissected in the median region, post-fixed in 1% osmuin tetroxide and embedded in epoxy resin. Semithin (0.5 µm) serial transverse sections were stained with 1% toluidine blue for light microscopy study. The descriptive histology and the topographical and histometric aspects of the median region of the nerve were analyzed, including myelinated fiber density, myelinated fiber diameter distribution, g ratio and number of endoneural capillary vessels. The cochlear nerve is enveloped by the vestibular nerve when they join together. Both, the cochlear and the vestibular nerves join to form the vestibulocochlear nerve with a Y shape, with approximately 5 mm in length. The nerve is widely vascularized and presents a complete mixture of the vestibular and cochlear myelinated fibers when the VIII nerve fascicle is constituted. The myelinated fibers are longitudinally oriented and present few endoneural connective tissue in between. No unmyelinated fibers were evidenced in the VIII nerve. The myelinated fiber diameter distribution was unimodal, with a peak at 3.5, and intervals between 1,5 and 6 m. The average number of fibers was 5.390 ± 1.504. The myelinated axon diameter distribution was also unimodal, with a peak at 2.5 µm of diameter were predominant quantitatively: 6.757 ± 1.922, and intervals between 1 and 4.5 µm. The average myelinated fiber density was 41.474 ± 4384 mm2, spread in a fascicular diameter of 0.77 ± 0.04 mm. The g ratio values varied from 0.2 to 0.9 and most of the myelinated fibers showed g ratio values of 0.7. Our results are original and only few parameters studied here were described in the literature. The vestibulocochlear nerve of the guinea pigs is a special afferent nerve, differing from other peripheral nerves (sensitive and motor) in its main anatomic and physiological characteristics, compared to other nerves from mammals. The adult guinea pig is an excellent model for the vestibulocochlear nerve neuropathy studies once it is similar to the human nerve.
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Servière, Jacques. "La tonotopie du colliculus inferieur chez trois espèces de mammifères (chat, singe, cobaye) : étude anatomo-fonctionnelle par le 14c-2-désoxyglucose." Paris 6, 1986. http://www.theses.fr/1986PA066251.

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Turcotte, Joanne Claire. "Projection from the estrogen receptor-rich region of the hypothalamus to other estrogen receptor-containing sites in the female guinea pig brain." 1996. https://scholarworks.umass.edu/dissertations/AAI9639044.

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Sexual behavior in female guinea pigs and rats is dependent upon circulating ovarian steroid hormones. The actions of these hormones on behavior are mediated by intracellular receptors located within interconnected brain regions. This complex neural network integrates somatosensory and hormonal information relevant to sexual behavior. An understanding of the anatomy of this network is important for understanding how behavior is generated. This dissertation investigated the neural projections from a region important for the induction of sexual behavior, the ventrolateral hypothalamus. Based on the steroid-sensitive neural network model described in rats, several experiments were designed to test predictions of this neural model in guinea pigs. In the first experiment, estrogen receptor- and estradiol-induced progestin receptor-containing cells were localized in the midbrain, a major projection site of the steroid receptor-rich region within the ventrolateral hypothalamus. In the second experiment, the anterograde tracer Phaseolus vulgaris-Leucoagglutinin, an anterograde tract-tracer, was deposited within the estrogen receptor-containing region of the ventrolateral hypothalamus. Projections from this area were found in most other estrogen receptor-containing sites, including the midbrain central gray, often closely associated with estrogen receptor-containing cells. The third experiment examined the distributions of substance P, a peptide found in ovarian steroid hormone receptor-containing cells in the ventrolateral hypothalamus and estrogen receptor-containing cells in the midbrain central gray. Substance P-immunoreactive boutons were found closely associated with some estrogen receptor-containing cells suggesting substance P modulation of ovarian steroid hormone receptor-containing cells. These connections, taken together with the hypothalamic projections closely associated with estrogen receptor-containing cells in the midbrain, support the idea that some estrogen receptor-containing cells may be directly linked. These studies provide information on the neural connections between estrogen receptor-containing regions and cells which may be important for regulating functions of the steroid hormone sensitive neural network.
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Books on the topic "Guinea pigs Anatomy"

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Gail, Saunders-Smith, ed. Pet guinea pigs up close. North Mankato, Minnesota: Capstone Press, a Capstone imprint, 2015.

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McCracken, Thomas. Color atlas of small animal anatomy: The essentials. Ames, Iowa: Blackwell Pub., 2008.

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A colour atlas of the anatomy of small laboratory animals. London: Wolfe Pub., 1992.

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A colour atlas of the anatomy of small laboratory animals. London: Saunders, 2002.

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Carlson, David, Thomas O. McCracken, and Robert A. Kainer. Color Atlas of Small Animal Anatomy: The Essentials. Wiley & Sons, Incorporated, John, 2013.

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Carlson, David, Thomas O. McCracken, and Robert A. Kainer. Color Atlas of Small Animal Anatomy: The Essentials. Wiley & Sons, Incorporated, John, 2013.

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Color Atlas Of Small Animal Anatomy The Essentials. Wiley-Blackwell, 2009.

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Carlson, David, Thomas O. McCracken, and Robert A. Kainer. Color Atlas of Small Animal Anatomy: The Essentials. Wiley & Sons, Incorporated, John, 2013.

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Rajtova, Viera, Jindrich Horak, and Peter Popesko. Colour Atlas of Anatomy of Small Laboratory Animals: Volume 1. Saunders Ltd., 2003.

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Rajtova, Viera, Jindrich Horak, and Peter Popesko. Colour Atlas of Anatomy of Small Laboratory Animals: Volume 2. Saunders Ltd., 2003.

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Book chapters on the topic "Guinea pigs Anatomy"

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Braitenberg, Valentino, and Almut Schüz. "Postnatal Changes, Possibly Due to Learning, in the Guinea Pig Cortex." In Anatomy of the Cortex, 131–39. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-662-02728-8_25.

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"Guinea pigs." In Clinical Anatomy and Physiology of Exotic Species, 197–208. Elsevier, 2005. http://dx.doi.org/10.1016/b978-070202782-6.50012-0.

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Alworth, Leanne C., and Stephen B. Harvey. "Anatomy, Physiology, and Behavior." In The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents, 955–66. Elsevier, 2012. http://dx.doi.org/10.1016/b978-0-12-380920-9.00039-0.

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Sohn, Joanne, and Marcelo A. Couto. "Anatomy, Physiology, and Behavior." In The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents, 195–215. Elsevier, 2012. http://dx.doi.org/10.1016/b978-0-12-380920-9.00008-0.

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Hargaden, Maureen, and Laura Singer. "Anatomy, Physiology, and Behavior." In The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents, 575–602. Elsevier, 2012. http://dx.doi.org/10.1016/b978-0-12-380920-9.00020-1.

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Murray, Kathleen A. "Anatomy, Physiology, and Behavior." In The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents, 753–63. Elsevier, 2012. http://dx.doi.org/10.1016/b978-0-12-380920-9.00027-4.

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