Academic literature on the topic 'GUVs'

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Journal articles on the topic "GUVs"

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Mushenheim, Peter C., Joel S. Pendery, Douglas B. Weibel, Saverio E. Spagnolie, and Nicholas L. Abbott. "Straining soft colloids in aqueous nematic liquid crystals." Proceedings of the National Academy of Sciences 113, no. 20 (2016): 5564–69. http://dx.doi.org/10.1073/pnas.1600836113.

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Liquid crystals (LCs), because of their long-range molecular ordering, are anisotropic, elastic fluids. Herein, we report that elastic stresses imparted by nematic LCs can dynamically shape soft colloids and tune their physical properties. Specifically, we use giant unilamellar vesicles (GUVs) as soft colloids and explore the interplay of mechanical strain when the GUVs are confined within aqueous chromonic LC phases. Accompanying thermal quenching from isotropic to LC phases, we observe the elasticity of the LC phases to transform initially spherical GUVs (diameters of 2–50 µm) into two disti
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Carvalho, Denise, Ana Rodrigues, Vera Faustino, Diana Pinho, Elisabete Castanheira, and Rui Lima. "Microfluidic Deformability Study of an Innovative Blood Analogue Fluid Based on Giant Unilamellar Vesicles." Journal of Functional Biomaterials 9, no. 4 (2018): 70. http://dx.doi.org/10.3390/jfb9040070.

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Blood analogues have long been a topic of interest in biofluid mechanics due to the safety and ethical issues involved in the collection and handling of blood samples. Although the current blood analogue fluids can adequately mimic the rheological properties of blood from a macroscopic point of view, at the microscopic level blood analogues need further development and improvement. In this work, an innovative blood analogue containing giant unilamellar vesicles (GUVs) was developed to mimic the flow behavior of red blood cells (RBCs). A natural lipid mixture, soybean lecithin, was used for the
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Garten, Matthias, Lars D. Mosgaard, Thomas Bornschlögl, Stéphane Dieudonné, Patricia Bassereau, and Gilman E. S. Toombes. "Whole-GUV patch-clamping." Proceedings of the National Academy of Sciences 114, no. 2 (2016): 328–33. http://dx.doi.org/10.1073/pnas.1609142114.

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Studying how the membrane modulates ion channel and transporter activity is challenging because cells actively regulate membrane properties, whereas existing in vitro systems have limitations, such as residual solvent and unphysiologically high membrane tension. Cell-sized giant unilamellar vesicles (GUVs) would be ideal for in vitro electrophysiology, but efforts to measure the membrane current of intact GUVs have been unsuccessful. In this work, two challenges for obtaining the “whole-GUV” patch-clamp configuration were identified and resolved. First, unless the patch pipette and GUV pressur
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Souissi, Mariem, Julien Pernier, Olivier Rossier, et al. "Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls." International Journal of Molecular Sciences 22, no. 12 (2021): 6335. http://dx.doi.org/10.3390/ijms22126335.

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Giant unilamellar vesicles (GUV) are powerful tools to explore physics and biochemistry of the cell membrane in controlled conditions. For example, GUVs were extensively used to probe cell adhesion, but often using non-physiological linkers, due to the difficulty of incorporating transmembrane adhesion proteins into model membranes. Here we describe a new protocol for making GUVs incorporating the transmembrane protein integrin using gel-assisted swelling. We report an optimised protocol, enumerating the pitfalls encountered and precautions to be taken to maintain the robustness of the protoco
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Sych, Taras, Thomas Schubert, Romain Vauchelles, et al. "GUV-AP: multifunctional FIJI-based tool for quantitative image analysis of Giant Unilamellar Vesicles." Bioinformatics 35, no. 13 (2018): 2340–42. http://dx.doi.org/10.1093/bioinformatics/bty962.

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Abstract Motivation Giant Unilamellar Vesicles (GUVs) are widely used synthetic membrane systems that mimic native membranes and cellular processes. Various fluorescence imaging techniques can be employed for their characterization. In order to guarantee a fast and unbiased analysis of imaging data, the development of automated recognition and processing steps is required. Results We developed a fast and versatile Fiji-based macro for the analysis of digital microscopy images of GUVs. This macro was designed to investigate membrane dye incorporation and protein binding to membranes. Moreover,
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Litschel, Thomas, and Petra Schwille. "Protein Reconstitution Inside Giant Unilamellar Vesicles." Annual Review of Biophysics 50, no. 1 (2021): 525–48. http://dx.doi.org/10.1146/annurev-biophys-100620-114132.

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Giant unilamellar vesicles (GUVs) have gained great popularity as mimicries for cellular membranes. As their sizes are comfortably above the optical resolution limit, and their lipid composition is easily controlled, they are ideal for quantitative light microscopic investigation of dynamic processes in and on membranes. However, reconstitution of functional proteins into the lumen or the GUV membrane itself has proven technically challenging. In recent years, a selection of techniques has been introduced that tremendously improve GUV-assay development and enable the precise investigation of p
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Karal, Mohammad Abu Sayem, Tawfika Nasrin, Marzuk Ahmed, et al. "A new purification technique to obtain specific size distribution of giant lipid vesicles using dual filtration." PLOS ONE 16, no. 7 (2021): e0254930. http://dx.doi.org/10.1371/journal.pone.0254930.

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A new purification technique is developed for obtaining distribution of giant unilamellar vesicles (GUVs) within a specific range of sizes using dual filtration. The GUVs were prepared using well known natural swelling method. For filtration, different combinations of polycarbonate membranes were implemented in filter holders. In our experiment, the combinations of membranes were selected with corresponding pore sizes–(i) 12 and 10 μm, (ii) 12 and 8 μm, and (iii) 10 and 8 μm. By these filtration arrangements, obtained GUVs size distribution were in the ranges of 6−26 μm, 5–38 μm and 5–30 μm, r
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Gaul, Vinnie, Sergio G. Lopez, Barry R. Lentz, Niamh Moran, Robert J. Forster та Tia E. Keyes. "The lateral diffusion and fibrinogen induced clustering of platelet integrin αIIbβ3 reconstituted into physiologically mimetic GUVs". Integrative Biology 7, № 4 (2015): 402–11. http://dx.doi.org/10.1039/c5ib00003c.

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A novel method for reconstitution of Integrin α<sub>IIb</sub>β<sub>3</sub> into GUVs with unrestricted lipid composition is described and the lateral diffusion and phase partitioning of the integrin on activation and ligand binding in biomimetic GUVs compositions is studied in GUVs with biomimetic formulations.
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Sarkar, Malay Kumar, Mohammad Abu Sayem Karal, Marzuk Ahmed, et al. "Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles." PLOS ONE 16, no. 5 (2021): e0251690. http://dx.doi.org/10.1371/journal.pone.0251690.

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Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique under different osmotic pressures (Π), and estimated the membrane tension due to Π. Two categories of GUVs were used in this study. One was prepared with a mixture of dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) and cholesterol (chol) for obtaining more biological relevance while other with a mixture of DOPG and DOPC,
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Jennings, Christopher S., Jeremy S. Rossman, Braeden A. Hourihan, Ross J. Marshall, Ross S. Forgan, and Barry A. Blight. "Immobilising giant unilamellar vesicles with zirconium metal–organic framework anchors." Soft Matter 17, no. 8 (2021): 2024–27. http://dx.doi.org/10.1039/d0sm02188a.

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A straightforward procedure for immobilising giant unilamellar vesicles (GUVs) using zircomium metal-organic frameworks as the anchroing medium is presented. Using this method GUVs can be immoblised and visualised for hours.
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Dissertations / Theses on the topic "GUVs"

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Manzini, Mariana Canale. "Efeito da carga dos lipídios na interação do BP100 em modelos de membrana." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-28032012-085459/.

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Os peptídeos antimicrobianos (AMPs) são uma alternativa promissora aos antibiróticos. Os AMPs, compostos de menos de 80 aminoácidos, apresentam caráter anfipático o que lhes dá a capacidade de agir em membranas lipídicas. Podem formar poros na membrana causando morte da célula. Seu espectro de ação é variado, sendo seus alvos bactérias até células de mamíferos. Os AMPs são encontrados em todos os seres vivos, desde bactérias, plantas, insetos e mamíferos, apresentam diferentes estruturas, composição de aminoácidos e agem por diversos mecanismos. Dois peptídeos antimicrobianos têm sido estudado
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Karamdad, Kaiser. "Development of microfluidic platforms to construct giant unilamellar vesicles (GUVs) for the biophysical study of lipid membranes." Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/56915.

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This thesis presents the design, development and application of several platforms through which to generate giant vesicles for biophysical and mechanical membrane studies. There has been a growing focus on manufacturing model membrane systems with improved throughput and structural properties in recent years. GUVs are a popular model membrane system for studying lipid membrane-associated phenomena due to their inherent similarity to biological cells. Traditional methods to construct vesicles offer little control over nuanced membrane properties such as asymmetry and patterning, which has paved
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Fagla-Amoussou, Akouavi Balbine. "Etude des interactions polluants aromatiques polycycliques (HAP)-récepteurs adrénergiques-phospholipides membranaires dans le tissu adipeux." Thesis, Vandoeuvre-les-Nancy, INPL, 2010. http://www.theses.fr/2010INPL080N/document.

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L'obésité est une maladie définie par une accumulation de masse grasse dans le tissu adipeux ayant des conséquences néfastes pour la santé. Les causes de l’obésité sont multiples. Dans un travail récent, il y a été démontré le rôle de la pollution environnementale dans la prise de poids. Dans ce travail, les hypothèses selon lesquelles les récepteurs adrénergiques situés à la surface des cellules adipeuses seraient le siège de l’action des polluants aromatiques polycycliques ont été vérifiées par le dosage de plusieurs agonistes et antagonistes spécifiques et non spécifiques en présence ou non
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Checchia, Robert Garcia. "Estudo de propriedades biofísicas de membrana sob estresse oxidativo e a interação com proteínas formadoras de poros." Universidade de São Paulo, 2019. http://www.teses.usp.br/teses/disponiveis/43/43134/tde-21032019-171115/.

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Neste trabalho investigamos efeitos de fotoirradiação e toxinas sob membranas celulares miméticas. Foram utilizadas, como modelo de membranas lipídicas, vesiculas unilamelares gigantes (GUVs) compostas pro lipídeos oxidados e não oxidados observadas por microscopia ótica de contraste de fase. Inicialmente estudamos a foto-resposta de membranas compostas por POPC e POPG dispersas em solução contendo azul de metileno (MB). Na sequência, estudamos o efeito de toxinas formadoras de poros, Esticolisina I (ST I) e Esticolisina II (ST II), em membranas contendo lipídeos oxidados e não oxidados. Os
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Domingues, Tatiana Moreira [UNIFESP]. "Síntese, estudos conformacionais e do mecanismo de ação da gomesina." Universidade Federal de São Paulo (UNIFESP), 2010. http://repositorio.unifesp.br/handle/11600/10093.

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Made available in DSpace on 2015-07-22T20:50:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-01-27. Added 1 bitstream(s) on 2015-08-11T03:25:32Z : No. of bitstreams: 1 Publico-00399a.pdf: 1787027 bytes, checksum: 6cb684251c1894e3685b611ace5c9056 (MD5). Added 1 bitstream(s) on 2015-08-11T03:25:32Z : No. of bitstreams: 2 Publico-00399a.pdf: 1787027 bytes, checksum: 6cb684251c1894e3685b611ace5c9056 (MD5) Publico-00399b.pdf: 1285455 bytes, checksum: 52050426f68cebcd4b7e00081ce49a38 (MD5). Added 1 bitstream(s) on 2015-08-11T03:25:32Z : No. of bitstreams: 3 Publico-00399a.pdf: 17870
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Valentino, Fabrice. "Systèmes biomimétiques pour l'étude du changement de forme cellulaire." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCC208/document.

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Le transport intracellulaire met en jeu des vésicules et nécessite ainsi des modifications de la membrane plasmique. En particulier, des nanotubes de membrane de quelques dizaines de nanomètres peuvent se former. Nous avons mis en place un système biomimétique à base de liposomes pour décrypter les mécanismes de changement de forme membranaire, en particulier sous l’action du cytosquelette d’actine. La physique des tubes de membrane est bien connue, notamment la force nécessaire au maintien de ce type de tube, qui dépend de l’élasticité de courbure du liposome et de sa tension de membrane impo
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Aimon, Sophie. "Study of a voltage-gated ion channel reconstituted in Giant Unilamellar Vesicles." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2011. http://tel.archives-ouvertes.fr/tel-00736743.

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Il est difficile d'étudier in vivo le rôle de la membrane dans l'excitabilité des cellules car les paramètres pertinents (composition et état mécanique de la membrane, densité de canaux...) sont activement régulés par la cellule elle-même et donc difficilement ajustables expérimentalement. J'ai donc développé une méthode pour reconstituer un canal voltage-dépendant dans une membrane où ces paramètres peuvent être contrôlés. Pour cela j'ai exprimé, purifié et marqué KvAP, un canal potassique voltage-dépendant. J'ai ensuite adapté une méthode existante pour le reconstituer dans des Vésicules Uni
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Maximino, Mateus Dassie [UNESP]. "Interação entre modelos de membrana biológica e poluentes emergentes." Universidade Estadual Paulista (UNESP), 2018. http://hdl.handle.net/11449/153668.

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Salgado, Rodolfo Jr. "Bubble guts enterprizes." Thesis, University of Iowa, 2012. https://ir.uiowa.edu/etd/2976.

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Akca, Uljana. "Hegel och Guds död." Thesis, Södertörns högskola, Institutionen för kultur och kommunikation, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-16268.

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In this study, I will discuss Hegel’s words on the death of God as they are stated in his Phenomenology of Spirit and Faith and Knowledge. I will present a reading of what I consider to be the two different usages of these words, and relate their meanings to each other. Focus lies on what Hegel refers to as the “unhappy consciousness” and its experience of a disenchantment of the world, and on Christianity as the answer to this position. The discussion will be grounded on Hegel’s dialectical understanding of the concept of God. The divine and the human nature, must, according to him, be graspe
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Books on the topic "GUVs"

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Lutz, Robert A. Guts. John Wiley & Sons, Ltd., 2003.

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stellwag, nevaeh alicen marie, ed. guts. scholastic, 2019.

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Guns. Wayland, 1988.

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Sim, Dave. Guys. Aardvark-Vanaheim Inc., 1997.

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Laymon, Richard. Guts. Learning Development Aids, 1996.

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Guns. Bookwright Press, 1988.

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Nylen, Robert. Guts. Random House Publishing Group, 2009.

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McBain, Ed. Guns. Curley Pub., 1992.

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McBain, Ed. Guns. Curley Pub, 1992.

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Nocera, Joseph. Good Guys and Bad Guys. Penguin Group USA, Inc., 2008.

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Book chapters on the topic "GUVs"

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Rols, Marie-Pierre. "Molecular Transmembrane Transport with Giant Unilamellar Vesicles (GUVs)." In Handbook of Electroporation. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-26779-1_84-1.

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Rols, Marie-Pierre. "Molecular Transmembrane Transport with Giant Unilamellar Vesicles (GUVs)." In Handbook of Electroporation. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-32886-7_84.

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Gooch, Jan W. "Gums." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_5725.

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Wagner, Michael. "Guns." In The English Chartered Trading Companies, 1688–1763. Routledge, 2018. http://dx.doi.org/10.4324/9780429464935-2.

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Kaushik, Mrinal, and Prashanth Reddy Hanmaiahgari. "Guns." In Essentials of Aircraft Armaments. Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-2377-4_2.

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Bode, Erasmus. "Guss." In Konstruktionsatlas. Vieweg+Teubner Verlag, 1996. http://dx.doi.org/10.1007/978-3-663-16320-6_5.

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BeMiller, J. N. "Gums." In Natural Products of Woody Plants. Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74075-6_27.

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Bouza, Anthony V. "Guns." In How to Stop Crime. Springer US, 1993. http://dx.doi.org/10.1007/978-1-4899-6483-0_7.

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de Oliveira, Weslley Felix, Priscilla Barbosa Sales Albuquerque, Priscila Marcelino dos Santos Silva, Luana Cassandra Breitenbach Barroso Coelho, and Maria Tereza dos Santos Correia. "Gums." In Polysaccharides of Microbial Origin. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-35734-4_4-1.

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Bonato, Anthony, and Richard Nowakowski. "Good guys versus bad guys." In The Student Mathematical Library. American Mathematical Society, 2011. http://dx.doi.org/10.1090/stml/061/09.

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Conference papers on the topic "GUVs"

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Yoshitani, Takuya, and Masahito Yamazaki. "Water permeability of lipid membranes of GUVs and its dependence on actin cytoskeletons inside the GUVs." In 2008 International Symposium on Micro-NanoMechatronics and Human Science. IEEE, 2008. http://dx.doi.org/10.1109/mhs.2008.4752436.

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Rong, Xi, Kenneth M. Pryse, Jordan A. Whisler, et al. "Confidence Intervals for Estimation of the Concentration and Brightness of Multiple Diffusing Species." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80921.

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Lipid nanodomains in cell membranes are believed to play a significant role in replication of enveloped viruses such as bird flu and HIV and signaling mechanisms underlying pathological conditions such as cancer. However, the forces that govern the formation and availability of these “membrane rafts” are uncertain, and even their existence is questioned. The central challenge is that no suitable imaging modalities exist (Elson, et al., 2010). We are developing tools to characterize and visualize dynamics of lipid nanodomains on idealized systems called giant unilamellar vesicles (GUVs) using f
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Jiang, Yanfei, Guy M. Genin, Srikanth Singamaneni, and Elliot L. Elson. "Interfacial Phases on Giant Unilamellar Vesicles." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80942.

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Lipid nanodomains in cell membranes are believed to play a significant role in a number of critical cellular processes (Elson, et al., 2010). These include, for example, replication processes in enveloped viruses such as bird flu and HIV and signaling mechanisms underlying pathological conditions such as cancer. Due to the potential for developing new disease treatments through the control of these membrane rafts, the biophysics underlying their formation has been the subject of intense study, much of this focused on domain formation in giant unilamellar lipid vesicles (GUVs), a simplified mod
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Lianos, Dimitrious, and Jere Brown. "Hypervelocity coil guns, rail guns and light gas guns." In Space Programs and Technologies Conference and Exhibit. American Institute of Aeronautics and Astronautics, 1994. http://dx.doi.org/10.2514/6.1994-4627.

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Finin, Tim, and David Drager. "GUMS." In the workshop. Association for Computational Linguistics, 1986. http://dx.doi.org/10.3115/1077146.1077173.

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Adelsberger, Stephan, Anton Setzer, and Eric Walkingshaw. "Declarative GUIs." In PPDP '18: The 20th International Symposium on Principles and Practice of Declarative Programming. ACM, 2018. http://dx.doi.org/10.1145/3236950.3236962.

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Mynatt, Elizabeth D., and W. Keith Edwards. "Audio GUIs." In Conference companion. ACM Press, 1995. http://dx.doi.org/10.1145/223355.223441.

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Brandmueller, Felix, and Zhuying Li. "Guts Game." In CHI PLAY '17: The annual symposium on Computer-Human Interaction in Play. ACM, 2017. http://dx.doi.org/10.1145/3130859.3130866.

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Venerandi, Alessandro, Giovanni Quattrone, and Licia Capra. "Guns of Brixton." In Urb-IoT '16: The Second International Conference on IoT in Urban Space. ACM, 2016. http://dx.doi.org/10.1145/2962735.2962750.

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Ponce, William. "GUTs and Strings." In Third Latin American Symposium on High Energy Physics. Sissa Medialab, 2000. http://dx.doi.org/10.22323/1.005.0029.

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Reports on the topic "GUVs"

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Morrison, Walter F., John D. Knapton, and Melvin J. Bulman. Liquid Propellant Guns. Defense Technical Information Center, 1987. http://dx.doi.org/10.21236/ada188575.

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Bouton, Laurent, Paola Conconi, Francisco Pino, and Maurizio Zanardi. Guns and Votes. National Bureau of Economic Research, 2014. http://dx.doi.org/10.3386/w20253.

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Pogue, E. W., R. M. Boat, D. N. Holden, and J. R. Lopez. Electromagnetic targeting of guns. Office of Scientific and Technical Information (OSTI), 1996. http://dx.doi.org/10.2172/380324.

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Duggan, Mark. More Guns, More Crime. National Bureau of Economic Research, 2000. http://dx.doi.org/10.3386/w7967.

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Barro, Robert. New Classicals and Keynesians, or the Good Guys and the Bad Guys. National Bureau of Economic Research, 1989. http://dx.doi.org/10.3386/w2982.

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Gelfeld, Vicki, and Patty David. Girls and Guys Getaways: Infographic. AARP Research, 2015. http://dx.doi.org/10.26419/res.00113.002.

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Weigand, Timo. F-Theory Uplifts and GUTs. Office of Scientific and Technical Information (OSTI), 2010. http://dx.doi.org/10.2172/992460.

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M., Lemenih, and Kassa H. Gums and resins of Ethiopia. Center for International Forestry Research (CIFOR), 2011. http://dx.doi.org/10.17528/cifor/003359.

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Mulhollan, G. Common Sense Copper and RF Guns. Office of Scientific and Technical Information (OSTI), 2005. http://dx.doi.org/10.2172/839658.

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Blumenhagen, Ralph, Volker Braun, Thomas W. Grimm, and Timo Weigand. GUTs on Compact Type IIB Orientifolds. Office of Scientific and Technical Information (OSTI), 2008. http://dx.doi.org/10.2172/943484.

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