Relevant bibliographies by topics / H2- producing conditions

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Academic literature on the topic 'H2- producing conditions'

Author: Grafiati
Published: 10 March 2023

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Journal articles on the topic "H2- producing conditions"

1

Conrad, R., B. Schink, and T. J. Phelps. "Thermodynamics of H2-consuming and H2-producing metabolic reactions in diverse methanogenic environments under in situ conditions." FEMS Microbiology Letters 38, no. 6 (1986): 353–60. http://dx.doi.org/10.1111/j.1574-6968.1986.tb01748.x.

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2

Strąpoć, Dariusz, Flynn W. Picardal, Courtney Turich, et al. "Methane-Producing Microbial Community in a Coal Bed of the Illinois Basin." Applied and Environmental Microbiology 74, no. 8 (2008): 2424–32. http://dx.doi.org/10.1128/aem.02341-07.

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ABSTRACT A series of molecular and geochemical studies were performed to study microbial, coal bed methane formation in the eastern Illinois Basin. Results suggest that organic matter is biodegraded to simple molecules, such as H2 and CO2, which fuel methanogenesis and the generation of large coal bed methane reserves. Small-subunit rRNA analysis of both the in situ microbial community and highly purified, methanogenic enrichments indicated that Methanocorpusculum is the dominant genus. Additionally, we characterized this methanogenic microorganism using scanning electron microscopy and distribution of intact polar cell membrane lipids. Phylogenetic studies of coal water samples helped us develop a model of methanogenic biodegradation of macromolecular coal and coal-derived oil by a complex microbial community. Based on enrichments, phylogenetic analyses, and calculated free energies at in situ subsurface conditions for relevant metabolisms (H2-utilizing methanogenesis, acetoclastic methanogenesis, and homoacetogenesis), H2-utilizing methanogenesis appears to be the dominant terminal process of biodegradation of coal organic matter at this location.
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3

Sakai, Sanae, Hiroyuki Imachi, Yuji Sekiguchi, Akiyoshi Ohashi, Hideki Harada, and Yoichi Kamagata. "Isolation of Key Methanogens for Global Methane Emission from Rice Paddy Fields: a Novel Isolate Affiliated with the Clone Cluster Rice Cluster I." Applied and Environmental Microbiology 73, no. 13 (2007): 4326–31. http://dx.doi.org/10.1128/aem.03008-06.

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ABSTRACT Despite the fact that rice paddy fields (RPFs) are contributing 10 to 25% of global methane emissions, the organisms responsible for methane production in RPFs have remained uncultivated and thus uncharacterized. Here we report the isolation of a methanogen (strain SANAE) belonging to an abundant and ubiquitous group of methanogens called rice cluster I (RC-I) previously identified as an ecologically important microbial component via culture-independent analyses. To enrich the RC-I methanogens from rice paddy samples, we attempted to mimic the in situ conditions of RC-I on the basis of the idea that methanogens in such ecosystems should thrive by receiving low concentrations of substrate (H2) continuously provided by heterotrophic H2-producing bacteria. For this purpose, we developed a coculture method using an indirect substrate (propionate) in defined medium and a propionate-oxidizing, H2-producing syntroph, Syntrophobacter fumaroxidans, as the H2 supplier. By doing so, we significantly enriched the RC-I methanogens and eventually obtained a methanogen within the RC-I group in pure culture. This is the first report on the isolation of a methanogen within RC-I.
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Posewitz, M. C., P. W. King, S. L. Smolinski, et al. "Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii." Biochemical Society Transactions 33, no. 1 (2005): 102–4. http://dx.doi.org/10.1042/bst0330102.

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The eukaryotic green alga, Chlamydomonas reinhardtii, produces H2 under anaerobic conditions, in a reaction catalysed by an [FeFe]-hydrogenase. To identify genes that influence H2 production in C. reinhardtii, a library of 6000 colonies on agar plates was screened with sensitive chemochromic H2-sensor films for clones defective in H2 production. Two mutants of particular interest were fully characterized. One mutant, hydEF-1, is unable to assemble an active [FeFe]-hydrogenase. This is the first reported C. reinhardtii mutant that is not capable of producing any H2. The second mutant, sta7-10, is not able to accumulate insoluble starch and has significantly lowered H2-photoproduction rates in comparison with the wild-type. In hydEF-1, anaerobiosis induces transcription of the two reported C. reinhardtii hydrogenase genes, HydA1 and HydA2, indicating a normal transcriptional response to anaerobiosis. In contrast, the transcription of both hydrogenase genes in sta7-10 is significantly attenuated.
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5

Pham, Hanh Thi Kim, Anh Thi Ngoc To, and Anh Duong Tam Nguyen. "Collection of some microbial consortia producing hydrogen from anaerobic wastes." Science and Technology Development Journal 16, no. 1 (2013): 51–59. http://dx.doi.org/10.32508/stdj.v16i1.1396.

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The preparation of hydrogen-producing microbial consortia from three anaerobic digested sludges were carried out by four different pretreatment methods (heat – shock, acid, base and aeration treatment) as well as untreatment. The obtained microbial seeds have been estimated for their stability in fermentative hydrogen production by three consecutive batch fermentations under the same conditions of pH 6.5, room temperature and cultivation time and also investigated the H2 fermentation from different concentrations of glucose and xylose. Three microbial seeds have the most effective H2 production at 5 g/l of glucose or xylose after 48 h cultivation time. The sewage sludge pretreated at 80oC for 30 minutes shows the hydrogen yield of 1.27 mol/mol glucose and 0.82 mol/mol xylose. The sludge in the biogas tank pretreated at 60oC for 30 minutes has the hydrogen yield of 1.27 mol/mol glucose and 0.71 mol/mol xylose. The sludge of the Hoa Binh waste treatment plant pretreated at 60oC for 30 minutes presents the hydrogen yield of 1.31 mol/mol glucose and 0.66 mol/mol xylose.
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6

Subramanian, Venkataramanan, Alexandra Dubini, David P. Astling, et al. "ProfilingChlamydomonasMetabolism under Dark, Anoxic H2-Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach." Journal of Proteome Research 13, no. 12 (2014): 5431–51. http://dx.doi.org/10.1021/pr500342j.

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7

Bakonyi, Péter, Nándor Nemestóthy, and Katalin Bélafi-Bakó. "Comparative Study of VariousE. coliStrains for Biohydrogen Production Applying Response Surface Methodology." Scientific World Journal 2012 (2012): 1–7. http://dx.doi.org/10.1100/2012/819793.

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The proper strategy to establish efficient hydrogen-producing biosystems is the biochemical, physiological characterization of hydrogen-producing microbes followed by metabolic engineering in order to give extraordinary properties to the strains and, finally, bioprocess optimization to realize enhanced hydrogen fermentation capability. In present paper, it was aimed to show the utility both of strain engineering and process optimization through a comparative study of wild-type and genetically modifiedE. colistrains, where the effect of two major operational factors (substrate concentration and pH) on bioH2production was investigated by experimental design and response surface methodology (RSM) was used to determine the suitable conditions in order to obtain maximum yields. The results revealed that by employing the genetically engineeredE. coli(DJT 135) strain under optimized conditions (pH: 6.5; Formate conc.: 1.25 g/L), 0.63 mol H2/mol formate could be attained, which was 1.5 times higher compared to the wild-typeE. coli(XL1-BLUE) that produced 0.42 mol H2/mol formate (pH: 6.4; Formate conc.: 1.3 g/L).
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8

Wang, Yuan Yuan, Jian Bo Wang, Cheng Xiao Hu, and Yan Lin Zhang. "Effect of Various Pretreatment Methods of Inoculum on Biohydrogen Production." Advanced Materials Research 152-153 (October 2010): 902–8. http://dx.doi.org/10.4028/www.scientific.net/amr.152-153.902.

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Influence of different pretreatment methods applied on anaerobic mixed inoculum was evaluated for selectively enriching the hydrogen (H2) producing mixed culture using glucose as substrate. The cumulative H2 yield and H2 production rate were found to be dependent on the type of pretreatment procedure adopted on the parent inoculum. They could be increased by appropriate pretreatment methods, including use of heat, alkaline or acidic conditions. Along with the processing temperature and time of heat pretreatment and alkaline of alkali pretreatment increasing, the H2 yield increased and then declined, but it declined and then increased as the acidity of acid pretreatment increasing. Among the studied pretreatment methods, the heat pretreatment methods procedure enabled higher H2 yield and the maximum H2 production rate, then were alkali and acid pretreatment methods. When the inoculum was heat-treated at 80°C for 30 min, the highest cumulative H2 yield was obtained at 2152.0 mL, which was 53.20% higher than the control, and the maximum H2 production rate was 178.0 mL h-1, which was 122.0% higher than that of the Ctrl (138.0mL h-1).
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9

Hartmann, L., D. Taras, B. Kamlage, and M. Blaut. "A new technique to determine hydrogen excreted by gnotobiotic rats." Laboratory Animals 34, no. 2 (2000): 162–70. http://dx.doi.org/10.1258/002367700780457617.

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A new system, that allowed the monitoring of hydrogen (H2) excretion by gnotobiotic rats without affecting their defined microbial status, was developed. The system consists of an isolator containing a chamber for an experimental animal, and a life-support system (LSS). with a sampling port outside the isolator connected to it. H2 accumulation in the system was measured by analysing a defined volume of gas after removal. H2 concentrations were determined with an electrochemical cell or by gas chromatography. To validate this technique, H2 excretion by germ-free (GF) and mono-associated rats fed a chemically defined diet was measured after oral application of lactulose. Mono-associated rats had been obtained by colonizing GF rats with a H2-producing Clostridium perfringens type A strain isolated from human faeces of a healthy volunteer. Application of 50 mg lactulose to the mono-associated rats resulted in a significant increase in H2 excretion. The net H2 excretion was 7.82±1.28 ml H2 in 12 h corresponding to a net maximal rate of 1.1±0.3 ml H2/h. In contrast, in experiments with GF rats, less than 0.13 ml H2 were detectable within 12 h. The technique presented is a useful tool for studying bacterial H2 metabolism in vivo under gnotobiotic conditions.
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10

Ipkawati, Nelda, Saktioto Saktioto, and Saktioto Saktioto. "PENENTUAN DENSITAS PLASMA HIDROGEN NONTERMAL PADA TEKANAN RENDAH." Komunikasi Fisika Indonesia 16, no. 1 (2019): 29. http://dx.doi.org/10.31258/jkfi.16.1.29-34.

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Before producing hydrogen plasma low pressure in experiment, it is necessary to know the density equilibrium process through a simulation. Hydrogen species densities of non-thermal plasma at low pressure is simulated using chemical kinetik model by Runge Kutta method. This simulation carried out to determine the equilibrium process of densities and reaction rates of hydrogen species in achieving equilibrium conditions. The equation used time-dependent continuity equation and Arrhenius form. The hydrogen species consist of electrons, H, H2, H+ and H2+. The results of show that electron density, H, H2, H+ and H2+ are respectively 1020,23m-3, 1019,69m-3, 1019,91m-3, 1019,39m-3 and 1018,43m-3 during of 23-24 ns. These describe that the density of each species of hydrogen very fast to achieve equilibrium conditions, while the value of the reaction rate obtained can be concluded that the value of the largest reaction rate is the impact ionization process with a value of 9.86x1052m-3 s-1and the smallest one is dissociation process with a value of 1.22x10-5m-3 s-1.
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