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Journal articles on the topic 'H9c2 cell differentiation'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Kankeu, Cynthia, Kylie Clarke, Delphi Van Haver, et al. "Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a ‘function follows form’ model of differentiation." Molecular Omics 14, no. 3 (2018): 181–96. http://dx.doi.org/10.1039/c8mo00036k.

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2

Sucharov, Carmen C., Stephen Langer, Michael Bristow, and Leslie Leinwand. "Shuttling of HDAC5 in H9C2 cells regulates YY1 function through CaMKIV/PKD and PP2A." American Journal of Physiology-Cell Physiology 291, no. 5 (2006): C1029—C1037. http://dx.doi.org/10.1152/ajpcell.00059.2006.

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YY1 is a transcription factor that can activate or repress transcription of a variety of genes and is involved in several developmental processes. YY1 is a repressor of transcription in differentiated H9C2 cells and in neonatal cardiac myocytes but an activator of transcription in undifferentiated H9C2 cells. We now present a detailed analysis of the functional domains of YY1 when it is acting as a repressor or an activator and identify the mechanism whereby its function is regulated in the differentiation of H9C2 cells. We show that histone deacetylase 5 (HDAC5) is localized to the cytoplasm

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3

Lama, Stefania, Vincenzo Monda, Maria Rosaria Rizzo, et al. "Cardioprotective Effects of Taurisolo® in Cardiomyoblast H9c2 Cells under High-Glucose and Trimethylamine N-Oxide Treatment via De Novo Sphingolipid Synthesis." Oxidative Medicine and Cellular Longevity 2020 (November 12, 2020): 1–11. http://dx.doi.org/10.1155/2020/2961406.

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In addition to high plasma glucose, increased levels of trimethylamine N-oxide (TMAO) have been found in obese subjects, where are considered as a novel risk factor for cardiovascular diseases. The present study aimed to investigate the effect of a novel nutraceutical formulation based on grape polyphenols (registered as Taurisolo®) in counteracting TMAO- and high glucose (HG)-induced cytotoxicity in cardiomyoblast H9c2 cells. Cell damage was induced with HG (HG-H9c2) and HG+TMAO (THG-H9c2); both experimental cell models were, thus, incubated for 72 h in the presence or absence of Taurisolo®.

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4

Ashrafi, Elham, Milica Radisic, and Janet A. W. Elliott. "Systematic cryopreservation study of cardiac myoblasts in suspension." PLOS ONE 19, no. 3 (2024): e0295131. http://dx.doi.org/10.1371/journal.pone.0295131.

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H9c2 myoblasts are a cell line derived from embryonic rat heart tissue and demonstrate the ability to differentiate to cardiac myotubes upon reduction of the serum concentration (from 10% to 1%) and addition of all-trans retinoic acid in the growth medium. H9c2 cells are increasingly being used as an easy-to-culture proxy for some functions of cardiomyocytes. The cryobiology of cardiac cells including H9c2 myoblasts has not been studied as extensively as that of some cell types. Consequently, it is important to characterize the cryobiological response and systematically develop well-optimized

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5

Zevolis, Evangelos, Anastassios Philippou, Athanasios Moustogiannis, Antonios Chatzigeorgiou, and Michael Koutsilieris. "The Effects of Mechanical Loading Variations on the Hypertrophic, Anti-Apoptotic, and Anti-Inflammatory Responses of Differentiated Cardiomyocyte-like H9C2 Cells." Cells 11, no. 3 (2022): 473. http://dx.doi.org/10.3390/cells11030473.

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Cardiomyocytes possess the ability to respond to mechanical stimuli by adapting their biological functions. This study investigated cellular and molecular events in cardiomyocyte-like H9C2 cells during differentiation as well as the signalling and gene expression responses of the differentiated cells under various mechanical stretching protocols in vitro. Immunofluorescence was used to monitor MyHC expression and structural changes during cardiomyoblast differentiation. Moreover, alterations in the expression of cardiac-specific markers, cell cycle regulatory factors, MRFs, hypertrophic, apopt

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6

Chen, Lena, Catherine S. W. Choi, Juan C. Sanchez-Arias, Laura T. Arbour, and Leigh Anne Swayne. "Ankyrin-B p.S646F undergoes increased proteasome degradation and reduces cell viability in the H9c2 rat ventricular cardiomyoblast cell line." Biochemistry and Cell Biology 98, no. 2 (2020): 299–306. http://dx.doi.org/10.1139/bcb-2019-0082.

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Ankyrin-B (AnkB) is scaffolding protein that anchors integral membrane proteins to the cardiomyocyte cytoskeleton. We recently identified an AnkB variant, AnkB p.S646F (ANK2 c.1937 C>T) associated with a phenotype ranging from predisposition for cardiac arrhythmia to cardiomyopathy. AnkB p.S646F exhibited reduced expression levels in the H9c2 rat ventricular-derived cardiomyoblast cell line relative to wildtype AnkB. Here, we demonstrate that AnkB is regulated by proteasomal degradation and proteasome inhibition rescues AnkB p.S646F expression levels in H9c2 cells, although this effect is n

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7

Pagano, M., S. Naviglio, A. Spina, et al. "Differentiation of H9c2 cardiomyoblasts: The role of adenylate cyclase system." Journal of Cellular Physiology 198, no. 3 (2004): 408–16. http://dx.doi.org/10.1002/jcp.10420.

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8

Giacomo, Viviana di, Monica Rapino, Silvia Sancilio та ін. "PKC-δ signalling pathway is involved in H9c2 cells differentiation". Differentiation 80, № 4-5 (2010): 204–12. http://dx.doi.org/10.1016/j.diff.2010.06.002.

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9

Sumi, Daigo, Kazusa Abe, and Seiichiro Himeno. "Arsenite retards the cardiac differentiation of rat cardiac myoblast H9c2 cells." Biochemical and Biophysical Research Communications 436, no. 2 (2013): 175–79. http://dx.doi.org/10.1016/j.bbrc.2013.05.069.

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10

Hu, Wei-Syun, Wei-Yu Liao, Chin-Hsien Chang, and Tung-Sheng Chen. "Paracrine IGF-1 Activates SOD2 Expression and Regulates ROS/p53 Axis in the Treatment of Cardiac Damage in D-Galactose-Induced Aging Rats after Receiving Mesenchymal Stem Cells." Journal of Clinical Medicine 11, no. 15 (2022): 4419. http://dx.doi.org/10.3390/jcm11154419.

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Aging is one of the causative agents associated with heart failure. Cell-based therapies show potential in the treatment of cardiac aging due to the characteristics of stem cells, including differentiation and the paracrine effect. This study aimed to investigate in detail the mechanism related to biomolecules released from mesenchymal stem cells in the treatment of cardiac aging. In vitro and in vivo models were designed to explore the above hypothesis. Experimental results from the in vitro model indicated that the elevation of oxidative stress, the expression of aging marker p53, and the su

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11

Bregant, Elisa, Giovanni Renzone, Renata Lonigro, et al. "Down-regulation of SM22/transgelin gene expression during H9c2 cells differentiation." Molecular and Cellular Biochemistry 327, no. 1-2 (2009): 145–52. http://dx.doi.org/10.1007/s11010-009-0052-2.

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12

Rocca, Carmine, Anna De Bartolo, Maria Concetta Granieri, et al. "The Antioxidant Selenoprotein T Mimetic, PSELT, Induces Preconditioning-like Myocardial Protection by Relieving Endoplasmic-Reticulum Stress." Antioxidants 11, no. 3 (2022): 571. http://dx.doi.org/10.3390/antiox11030571.

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Oxidative stress and endoplasmic reticulum stress (ERS) are strictly involved in myocardial ischemia/reperfusion (MI/R). Selenoprotein T (SELENOT), a vital thioredoxin-like selenoprotein, is crucial for ER homeostasis and cardiomyocyte differentiation and protection, likely acting as a redox-sensing protein during MI/R. Here, we designed a small peptide (PSELT), encompassing the redox site of SELENOT, and investigated whether its pre-conditioning cardioprotective effect resulted from modulating ERS during I/R. The Langendorff rat heart model was employed for hemodynamic analysis, while mechani

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13

van den Eijnde, Stefan M., Maurice J. B. van den Hoff, Chris P. M. Reutelingsperger, et al. "Transient expression of phosphatidylserine at cell-cell contact areas is required for myotube formation." Journal of Cell Science 114, no. 20 (2001): 3631–42. http://dx.doi.org/10.1242/jcs.114.20.3631.

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Cell surface exposure of phosphatidylserine (PS) is shown to be part of normal physiology of skeletal muscle development and to mediate myotube formation. A transient exposure of PS was observed on mouse embryonic myotubes at E13, at a stage of development when primary myotubes are formed. The study of this process in cell cultures of differentiating C2C12 and H9C2 myoblasts also reveals a transient expression of PS at the cell surface. This exposure of PS locates mainly at cell-cell contact areas and takes place at a stage when the structural organization of the sarcomeric protein titin is in

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14

Comelli, Marina, Rossana Domenis, Elena Bisetto, et al. "Cardiac differentiation promotes mitochondria development and ameliorates oxidative capacity in H9c2 cardiomyoblasts." Mitochondrion 11, no. 2 (2011): 315–26. http://dx.doi.org/10.1016/j.mito.2010.12.007.

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15

Branco, Ana F., Sandro L. Pereira, Ana C. Moreira, Jon Holy, Vilma A. Sardão, and Paulo J. Oliveira. "Isoproterenol Cytotoxicity is Dependent on the Differentiation State of the Cardiomyoblast H9c2 Cell Line." Cardiovascular Toxicology 11, no. 3 (2011): 191–203. http://dx.doi.org/10.1007/s12012-011-9111-5.

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16

Wu, Fang, Feng Wang, Qian Yang, et al. "Upregulation of miRNA-23a-3p rescues high glucose-induced cell apoptosis and proliferation inhibition in cardiomyocytes." In Vitro Cellular & Developmental Biology - Animal 56, no. 10 (2020): 866–77. http://dx.doi.org/10.1007/s11626-020-00518-6.

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AbstractMaternal hyperglycemia potentially inhibits the development of the fetal heart by suppressing cardiomyocyte proliferation and promoting apoptosis. Different studies have indicated that miRNAs are key regulators of cardiomyocyte proliferation, differentiation, and apoptosis and play a protective role in a variety of cardiovascular diseases. However, the biological function of miRNA-23a in hyperglycemia-related cardiomyocyte injury is not fully understood. The present study investigated the effect of miRNA-23a-3p on cell proliferation and apoptosis in a myocardial injury model induced by

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17

Hou, Qi, and Yi-Te Hsu. "Bax translocates from cytosol to mitochondria in cardiac cells during apoptosis: development of a GFP-Bax-stable H9c2 cell line for apoptosis analysis." American Journal of Physiology-Heart and Circulatory Physiology 289, no. 1 (2005): H477—H487. http://dx.doi.org/10.1152/ajpheart.00879.2004.

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The proapoptotic protein Bax plays an important role in cardiomyocytic cell death. Ablation of this protein has been shown to diminish cardiac damage in Bax-knockout mice during ischemia-reperfusion. Presently, studies of Bax-mediated cardiac cell death examined primarily the expression levels of Bax and its prosurvival factor Bcl-2 rather than the localization of this protein, which dictates its function. Using immunofluorescence labeling, we have shown that in neonatal rat cardiomyocytes and in H9c2 cardiomyoblasts, Bax translocates from cytosol to mitochondria upon the induction of apoptosi

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18

Borger, Moritz, Clarissa von Haefen, Christoph Bührer, and Stefanie Endesfelder. "Cardioprotective Effects of Dexmedetomidine in an Oxidative-Stress In Vitro Model of Neonatal Rat Cardiomyocytes." Antioxidants 12, no. 6 (2023): 1206. http://dx.doi.org/10.3390/antiox12061206.

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Preterm birth is a risk factor for cardiometabolic disease. The preterm heart before terminal differentiation is in a phase that is crucial for the number and structure of cardiomyocytes in further development, with adverse effects of hypoxic and hyperoxic events. Pharmacological intervention could attenuate the negative effects of oxygen. Dexmedetomidine (DEX) is an α2-adrenoceptor agonist and has been mentioned in connection with cardio-protective benefits. In this study, H9c2 myocytes and primary fetal rat cardiomyocytes (NRCM) were cultured for 24 h under hypoxic condition (5% O2), corresp

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19

Reis-Mendes, Ana, Marisa Alves, Félix Carvalho, Fernando Remião, Maria Lourdes Bastos, and Vera Marisa Costa. "Pixantrone, a new anticancer drug with the same old cardiac problems? An in vitro study with differentiated and non-differentiated H9c2 cells." Interdisciplinary Toxicology 11, no. 1 (2018): 13–21. http://dx.doi.org/10.2478/intox-2018-0002.

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Abstract Pixantrone (PIX) is an anticancer drug approved for the treatment of multiple relapsed or refractory aggressive B-cell non-Hodgkin’s lymphoma. It is an aza-anthracenedione synthesized to have the same anticancer activity as its predecessors, anthracyclines (e.g. doxorubicin) and anthracenediones (e.g. mitoxantrone), with lower cardiotoxicity. However, published data regarding its possible cardiotoxicity are scarce. Therefore, this work aimed to assess the potential cytotoxicity of PIX, at clinically relevant concentrations (0.1; 1; and 10 µM) in both non-differentiated and 7-day diffe

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20

Apostolova, Margarita D., Iordanka A. Ivanova, and M. George Cherian. "Signal transduction pathways, and nuclear translocation of zinc and metallothionein during differentiation of myoblasts." Biochemistry and Cell Biology 78, no. 1 (2000): 27–37. http://dx.doi.org/10.1139/o99-070.

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The changes in subcellular localization of metallothionein during differentiation were studied in two myoblast cell lines, L6 and H9C2. Addition of insulin like growth factor-I or lowering foetal bovine serum to 1% can induce differentiation of myoblasts to myotubes. Metallothionein and zinc were localized mainly in the cytoplasm in myoblasts but were translocated into the nucleus of newly formed myotubes during early differentiation. In fully differentiated myotubes, metallothionein content was decreased with a cytoplasmic localization. Addition of an inhibitor of mitogen-activated protein ki

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21

Boccellino, Mariarosaria, Giovanni Galasso, Pasqualina Ambrosio, et al. "H9c2 Cardiomyocytes under Hypoxic Stress: Biological Effects Mediated by Sentinel Downstream Targets." Oxidative Medicine and Cellular Longevity 2021 (September 30, 2021): 1–10. http://dx.doi.org/10.1155/2021/6874146.

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The association between diabetes and cardiovascular diseases is well known. Related diabetes macro- and microangiopathies frequently induce hypoxia and consequently energy failure to satisfy the jeopardized myocardium basal needs. Additionally, it is widely accepted that diabetes impairs endothelial nitric oxide synthase (eNOS) activity, resulting in diminished nitric oxide (NO) bioavailability and consequent endothelial cell dysfunction. In this study, we analyzed the embryonic heart-derived H9c2 cell response to hypoxic stress after administration of a high glucose concentration to reproduce

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22

Fang, Yao-Ching, and Chi-Hsiao Yeh. "Inhibition of miR-302 Suppresses Hypoxia-Reoxygenation-Induced H9c2 Cardiomyocyte Death by Regulating Mcl-1 Expression." Oxidative Medicine and Cellular Longevity 2017 (2017): 1–9. http://dx.doi.org/10.1155/2017/7968905.

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MicroRNAs play important roles in cell proliferation, differentiation, and apoptosis, and their expression influences cardiomyocyte apoptosis resulting from ischemia-induced myocardial infarction. Here, we determined the role of miR expression in cardiomyocyte apoptosis during hypoxia and reoxygenation. The rat cardiomyocyte cell line H9c2 was incubated for 3 h in normal or hypoxia medium, followed by reoxygenation for 24 h and transfection with a miR-302 mimic or antagomir. The effect of miR-302 on myeloid leukemia cell-differentiation protein-1 (Mcl-1) expression was determined by western bl

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23

Giusti, Laura, Claudia Gargini, Francesca Ceccarelli, Michela Bacci, Paola Italiani та Maria R. Mazzoni. "Modulation of Endothelin-A Receptor, Gα Subunit, and RGS2 Expression during H9c2 Cardiomyoblast Differentiation". Journal of Receptors and Signal Transduction 24, № 4 (2004): 297–317. http://dx.doi.org/10.1081/rrs-200040331.

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24

van der Putten, HH, BJ Joosten, PH Klaren, and ME Everts. "Uptake of tri-iodothyronine and thyroxine in myoblasts and myotubes of the embryonic heart cell line H9c2(2-1)." Journal of Endocrinology 175, no. 3 (2002): 587–96. http://dx.doi.org/10.1677/joe.0.1750587.

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Uptake of tri-iodothyronine (T(3)) was compared with that of thyroxine (T(4)) in the embryonic heart cell line H9c2 (2-1). These cells propagate as myoblasts and form differentiated myotubes upon reduction of the serum concentration, as indicated by a 31-fold increase in creatine kinase activity. Protein and DNA content per well were around 2-fold higher in myotubes than in myoblasts. When expressed per well, T(3) and T(4) uptake were, compared with myoblasts, 1.9- to 2-fold and 3.1- to 4-fold higher in myotubes respectively. On the other hand, the characteristics of T(3) and T(4) uptake were

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25

Branco, Ana F., Sandro L. Pereira, Ana C. Moreira, Jon Holy, Vilma A. Sardão, and Paulo J. Oliveira. "Erratum to: Isoproterenol Cytotoxicity is Dependent on the Differentiation State of the Cardiomyoblast H9c2 Cell Line." Cardiovascular Toxicology 11, no. 3 (2011): 284. http://dx.doi.org/10.1007/s12012-011-9131-1.

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26

Ménard, Claudine, Sandrine Pupier, Dominique Mornet, Magali Kitzmann, Joël Nargeot, and Philippe Lory. "Modulation of L-type Calcium Channel Expression during Retinoic Acid-induced Differentiation of H9C2 Cardiac Cells." Journal of Biological Chemistry 274, no. 41 (1999): 29063–70. http://dx.doi.org/10.1074/jbc.274.41.29063.

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27

Liu, Rongchen, Xiufang Gao, and Haiming Shi. "Impact of Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 on the Susceptibility of High Glucose-Treated Cardiomyocytes to Hypoxia/Reoxygenation." Journal of Biomaterials and Tissue Engineering 12, no. 10 (2022): 2006–13. http://dx.doi.org/10.1166/jbt.2022.3134.

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To estimate the effect of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on susceptibility of high glucose (HG)-treated cardiomyocytes (CMs) to hypoxia/reoxygenation (H/R). Forty healthy rats were assigned to the control, diabetes mellitus (DM), DM + ischemia/reperfusion injury (IRI) control, and DM IRI groups (n = 10 for each group). Rat CMs (H9C2) were subjected to HG and H/R treatments. LncRNA MALAT1 and cyclic-AMP responsive element modulator (CREM) mRNA levels were measured using quantitative polymerase chain reaction, and protein levels of CREM, myeloid differenti

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28

Navaee, Fatemeh, Philippe Renaud, Alexander Kleger, and Thomas Braschler. "Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix." International Journal of Molecular Sciences 24, no. 3 (2023): 2842. http://dx.doi.org/10.3390/ijms24032842.

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Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model. Rapid and specific coagulation with thrombin facilitates the gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. Our investigations revealed enhanced cardiogenic differentiation in the H9c2 myoblast cells when using the system in a co-culture with

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29

Hsu, Po-Shun, Shu-Ting Liu, Yi-Lin Chiu, and Chien-Sung Tsai. "The Functional Role of Myogenin in Cardiomyoblast H9c2 Cells Treated with High Glucose and Palmitic Acid: Insights into No-Rejection Heart Transplantation." International Journal of Molecular Sciences 24, no. 17 (2023): 13031. http://dx.doi.org/10.3390/ijms241713031.

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Various pathological alterations, including lipid-deposition-induced comparative cardiac lipotoxicity, contribute to cardiac aging in the failing heart. A decline in endogenous myogenin proteins can lead to the reversal of muscle cell differentiation and the creation of mononucleated muscle cells. Myogenin may be a specific regulator of adaptive responses to avoid pathological hypertrophy in the heart. Hence, it is important to understand the regulation of myogenin expression and functions in response to exposure to varied stresses. In this study, we first examined and verified the cytotoxic e

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30

Hong, Feng, Keun-ai Moon, Sam Soo Kim та ін. "Role of Phospholipase C-γ1 in Insulin-like Growth Factor I-Induced Muscle Differentiation of H9c2 Cardiac Myoblasts". Biochemical and Biophysical Research Communications 282, № 3 (2001): 816–22. http://dx.doi.org/10.1006/bbrc.2001.4644.

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31

Kageyama, Kan, Yoshito Ihara, Shinji Goto, et al. "Overexpression of Calreticulin Modulates Protein Kinase B/Akt Signaling to Promote Apoptosis during Cardiac Differentiation of Cardiomyoblast H9c2 Cells." Journal of Biological Chemistry 277, no. 22 (2002): 19255–64. http://dx.doi.org/10.1074/jbc.m112377200.

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32

Støle, Thea Parsberg, Marianne Lunde, Katja Gehmlich, Geir Christensen, William E. Louch, and Cathrine Rein Carlson. "Exploring Syndecan-4 and MLP and Their Interaction in Primary Cardiomyocytes and H9c2 Cells." Cells 13, no. 11 (2024): 947. http://dx.doi.org/10.3390/cells13110947.

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The transmembrane proteoglycan syndecan-4 is known to be involved in the hypertrophic response to pressure overload. Although multiple downstream signaling pathways have been found to be involved in this response in a syndecan-4-dependent manner, there are likely more signaling components involved. As part of a larger syndecan-4 interactome screening, we have previously identified MLP as a binding partner to the cytoplasmic tail of syndecan-4. Interestingly, many human MLP mutations have been found in patients with hypertrophic (HCM) and dilated cardiomyopathy (DCM). To gain deeper insight int

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33

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, and Snehasis Jana. "Elasticity Profile of Skin, Neuronal, Cardiac, and Skeletal Muscle Cells after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation." Journal of Biotechnology and Biomedical Science 2, no. 4 (2021): 19–29. http://dx.doi.org/10.14302/issn.2576-6694.jbbs-21-3819.

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The present study aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on elasticity of skin, heart, muscle, and neuronal cells in the H9C2 (rat cardiomyocytes), C2C12 (mouse myoblast cells), HaCaT (human keratinocytes), and SH-SY5Y (human neuroblastoma cells) cell line in DMEM medium. The test formulation constit

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34

Trivedi, Mahendra Kumar, Dahryn Trivedi, Snehasis Jana, and Alice Branton. "Elasticity Profile of Skin, Neuronal, Cardiac, and Skeletal Muscle Cells after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation." Journal of Biotechnology and Biomedical Science 2, no. 4 (2021): 19–30. https://doi.org/10.5281/zenodo.10297956.

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The present study aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on elasticity of skin, heart, muscle, and neuronal cells in the H9C2 (rat cardiomyocytes), C2C12 (mouse myoblast cells), HaCaT (human keratinocytes), and SH-SY5Y (human neuroblastoma cells) cell line in DMEM medium. The test formulation constit

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35

Trivedi, Mahendra Kumar, Dahryn Trivedi, Snehasis Jana, and Alice Branton. "Elasticity Profile of Skin, Neuronal, Cardiac, and Skeletal Muscle Cells after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation." Journal of Biotechnology and Biomedical Science 2, no. 4 (2021): 19–30. https://doi.org/10.5281/zenodo.10297962.

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The present study aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on elasticity of skin, heart, muscle, and neuronal cells in the H9C2 (rat cardiomyocytes), C2C12 (mouse myoblast cells), HaCaT (human keratinocytes), and SH-SY5Y (human neuroblastoma cells) cell line in DMEM medium. The test formulation constit

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36

Trivedi, Mahendra Kumar, Dahryn Trivedi, Snehasis Jana, and Alice Branton. "Elasticity Profile of Skin, Neuronal, Cardiac, and Skeletal Muscle Cells after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation." Journal of Biotechnology and Biomedical Science 2, no. 4 (2021): 19–30. https://doi.org/10.14302/issn.2576-6694.jbbs-21-3819.

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The present study aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on elasticity of skin, heart, muscle, and neuronal cells in the H9C2 (rat cardiomyocytes), C2C12 (mouse myoblast cells), HaCaT (human keratinocytes), and SH-SY5Y (human neuroblastoma cells) cell line in DMEM medium. The test formulation constit

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37

Frati, Alessia, Barbara Ricci, Federica Pierucci, Silvia Nistri, Daniele Bani, and Elisabetta Meacci. "Role of Sphingosine Kinase/S1P Axis in ECM Remodeling of Cardiac Cells Elicited by Relaxin." Molecular Endocrinology 29, no. 1 (2015): 53–67. http://dx.doi.org/10.1210/me.2014-1201.

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Abstract The initiation and progression of heart failure is linked to adverse cardiac remodeling of the extracellular matrix (ECM) during disease mainly through the deregulation of myocardial metalloproteinases (MMPs). Relaxin (RLX), a peptide hormone acting as a physiological cardiac effector, is a key regulator of ECM remodeling in reproductive and nonreproductive tissues. Studying primary cultures of mouse cardiac muscle cells and rat H9c2 cardiomyoblasts, we have obtained evidence for a new signaling pathway activated by RLX to induce ECM remodeling that involves the bioactive sphingolipid

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38

Gao, Jin-meng, Xiao-wen Meng, Juan Zhang та ін. "Dexmedetomidine Protects Cardiomyocytes against Hypoxia/Reoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling". BioMed Research International 2017 (2017): 1–13. http://dx.doi.org/10.1155/2017/1674613.

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Objective. We previously reported that dexmedetomidine (DEX) offers cardioprotection against ischemia/reperfusion injury in rats. Here, we evaluated the role of toll-like receptors 4- (TLR4-) myeloid differentiation primary response 88- (MyD88-) nuclear factor-kappa B (NF-κB) signaling in DEX-mediated protection of cardiomyocytes usingin vitromodels of hypoxia/reoxygenation (H/R).Methods. The experiments were carried out in H9C2 cells and in primary neonatal rat cardiomyocytes. Cells pretreated with vehicle or DEX were exposed to hypoxia for 1 h followed by reoxygenation for 12 h. We analyzed

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Rocca, Carmine, Anna De Bartolo, Rita Guzzi, et al. "Palmitate-Induced Cardiac Lipotoxicity Is Relieved by the Redox-Active Motif of SELENOT through Improving Mitochondrial Function and Regulating Metabolic State." Cells 12, no. 7 (2023): 1042. http://dx.doi.org/10.3390/cells12071042.

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Cardiac lipotoxicity is an important contributor to cardiovascular complications during obesity. Given the fundamental role of the endoplasmic reticulum (ER)-resident Selenoprotein T (SELENOT) for cardiomyocyte differentiation and protection and for the regulation of glucose metabolism, we took advantage of a small peptide (PSELT), derived from the SELENOT redox-active motif, to uncover the mechanisms through which PSELT could protect cardiomyocytes against lipotoxicity. To this aim, we modeled cardiac lipotoxicity by exposing H9c2 cardiomyocytes to palmitate (PA). The results showed that PSEL

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Liu, Szu-Yu, Chia-Chang Huang, Ying-Ying Yang, et al. "Obeticholic acid treatment ameliorates the cardiac dysfunction in NASH mice." PLOS ONE 17, no. 12 (2022): e0276717. http://dx.doi.org/10.1371/journal.pone.0276717.

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Background Suppression of cardiac iinflammasome, which can be inhibited by Farnesoid X receptor (FXR) agonist, can ameliorate cardiac inflammation and fibrosis. Increased cardiac inflammasome decrease the abundance of regulatory T (Treg) cells and exacerbate cardiac dysfunction. Interaction between cardiomyocytes and Treg cells is involved in the development of nonalcoholic steatohepatitis (NASH)-related cardiac dysfunction. Aims This study evaluates whether the FXR agonist obeticholic acid (OCA) treatment improves NASH-associated cardiac dysfunction. Methods The in vivo and in vitro mechanism

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Park, Jei Hyoung, Kyoung Jin Nho, Ji Young Lee та ін. "Anti-Ischemic Effects of PIK3IP1 Are Mediated through Its Interactions with the ETA-PI3Kγ-AKT Axis". Cells 11, № 14 (2022): 2162. http://dx.doi.org/10.3390/cells11142162.

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Oxidative stress, caused by the accumulation of reactive oxygen species (ROS) during acute myocardial infarction (AMI), is one of the main factors leading to myocardial cell damage and programmed cell death. Phosphatidylinositol-3-kinase-AKT (PI3K-AKT) signaling is essential for regulating cell proliferation, differentiation, and apoptosis. Phosphoinositide-3-kinase (PI3K)-interacting protein 1 (PIK3IP1) is an intrinsic inhibitor of PI3K in various tissues, but its functional role during AMI remains unknown. In this study, the anti-ischemic role of PIK3IP1 in an in vitro AMI setting was evalua

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Alloatti, Giuseppe, Elisa Arnoletti, Eleonora Bassino, et al. "Obestatin affords cardioprotection to the ischemic-reperfused isolated rat heart and inhibits apoptosis in cultures of similarly stressed cardiomyocytes." American Journal of Physiology-Heart and Circulatory Physiology 299, no. 2 (2010): H470—H481. http://dx.doi.org/10.1152/ajpheart.00800.2009.

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Obestatin, a newly discovered peptide encoded by the ghrelin gene, induces the expression of genes regulating pancreatic β-cell differentiation, insulin biosynthesis, and glucose metabolism. It also activates antiapoptotic signaling pathways such as phosphoinositide 3-kinase (PI3K) and ERK1/2 in pancreatic β-cells and human islets. Since these kinases have been shown to protect against myocardial injury, we sought to investigate whether obestatin would exert cardioprotective effects. Both isolated perfused rat heart and cultured cardiomyocyte models of ischemia-reperfusion (I/R) were used to m

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Sugarman, Eliot, Ada Koo, Eigo Suyama, et al. "Identification of Inhibitors of Triacylglyceride Accumulation in Muscle Cells." Journal of Biomolecular Screening 19, no. 1 (2013): 77–87. http://dx.doi.org/10.1177/1087057113501198.

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Excess caloric consumption leads to triacylglyceride (TAG) accumulation in tissues that do not typically store fat, such as skeletal muscle. This ectopic accumulation alters cells, contributing to the pathogenesis of metabolic syndrome, a major health problem worldwide. We developed a 1536-well assay to measure intracellular TAG accumulation in differentiating H9c2 myoblasts. For this assay, cells were incubated with oleic acid to stimulate TAG accumulation prior to adding compounds. We used Nile red as a fluorescent dye to quantify TAG content with a microplate reader. The cell nuclei were co

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Felemban, Shatha G., Falguni S. Vyas, Lyndsey Durose, Alan J. Hargreaves, and John M. Dickenson. "Phenyl Saligenin Phosphate Disrupts Cell Morphology and the Actin Cytoskeleton in Differentiating H9c2 Cardiomyoblasts and Human-Induced Pluripotent Stem-Cell-Derived Cardiomyocyte Progenitor Cells." Chemical Research in Toxicology 33, no. 9 (2020): 2310–23. http://dx.doi.org/10.1021/acs.chemrestox.0c00100.

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Klett, Hagen, Lonny Jürgensen, Patrick Most, et al. "Delineating the Dynamic Transcriptome Response of mRNA and microRNA during Zebrafish Heart Regeneration." Biomolecules 9, no. 1 (2018): 11. http://dx.doi.org/10.3390/biom9010011.

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Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating these mechanisms would improve health in patients suffering from long-term consequences of ischemia. Therefore, we monitored the dynamic transcriptome response of both mRNA and microRNA in zebrafish at 1–160 days post cryoinjury (dpi). Using a control model of sham-operated and healthy fish, we extract

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Wu, Yingxu, Xiaojing Deng, Lan Ye, Wei Zhang, Hu Xu, and Boyu Zhang. "A TCF-Based Carbon Monoxide NIR-Probe without the Interference of BSA and Its Application in Living Cells." Molecules 27, no. 13 (2022): 4155. http://dx.doi.org/10.3390/molecules27134155.

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As toxic gaseous pollution, carbon monoxide (CO) plays an essential role in many pathological and physiological processes, well-known as the third gasotransmitter. Owning to the reducibility of CO, the Pd0-mediated Tsuji-Trost reaction has drawn much attention in CO detection in vitro and in vivo, using allyl ester and allyl ether caged fluorophores as probes and PdCl2 as co-probes. Because of its higher decaging reactivity than allyl ether in the Pd0-mediated Tsuji-Trost reaction, the allyl ester group is more popular in CO probe design. However, during the application of allyl ester caged pr

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Campero-Basaldua, Carlos, Jessica Herrera-Gamboa, Judith Bernal-Ramírez, et al. "The retinoic acid response is a minor component of the cardiac phenotype in H9c2 myoblast differentiation." BMC Genomics 24, no. 1 (2023). http://dx.doi.org/10.1186/s12864-023-09512-0.

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AbstractThe H9c2 myoblast cell line, isolated from the left ventricular tissue of rat, is currently used in vitro as a mimetic for skeletal and cardiac muscle due to its biochemical, morphological, and electrical/hormonal signaling properties. During culture, H9c2 cells acquire a myotube phenotype, where a critical component is the inclusion of retinoic acid (RA). The results from some authors on H9c2 suggested that thousands of genes respond to RA stimuli, while others report hundreds of genes responding to RA over different cell types. In this article, using a more appropriate experimental d

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Brock, Judith, and Marcel Hörning. "Optimization of H9c2 differentiation leads to calcium-active and striated cardiac cells without addition of retinoic acid." Frontiers in Cell and Developmental Biology 12 (November 22, 2024). http://dx.doi.org/10.3389/fcell.2024.1501540.

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As a reliable alternative to animal testing in cardiovascular research, it is crucial to improve differentiation of immortalized cell lines. In this study, we focused on optimizing the differentiation efficiency of the H9c2 cell line into cardiomyocytes using a high-throughput, automated image processing approach. While previous studies used protocols involving retinoic acid to enhance cardiac differentiation, we applied a simplified medium composition that results in higher differentiation rates. Along that line, we differentiated H9c2 cells into cardiomyocytes, which not only showed sarcomer

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Fatemeh, Navaee, Renaud Philippe, and Braschler Thomas. "Highly efficient cardiac differentiation and maintenance by thrombin-coagulated fibrin hydrogels enriched with decellularized porcine heart extracellular matrix." May 21, 2020. https://doi.org/10.5281/zenodo.3838400.

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We provide a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen for the formation of an <em>in-vitro</em> 3D cardiac cell culture model. Rapid and specific coagulation with thrombin allows gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. We show enhanced cardiogenic differentiation in the H9c2 myoblast cell line when using the system in co-culture with Nor-10 fibroblasts. Further enhancement of differentiation efficiency is obtained by 3D embedding. We then proceed with culture of rat n

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Santos, Ana Elisa Antunes dos, Jorge Luís Guadalupe, Juliano Douglas Silva Albergaria, et al. "Random cellulose acetate nanofibers: a breakthrough for cultivated meat production." Frontiers in Nutrition 10 (January 5, 2024). http://dx.doi.org/10.3389/fnut.2023.1297926.

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Overcoming the challenge of creating thick, tissue-resembling muscle constructs is paramount in the field of cultivated meat production. This study investigates the remarkable potential of random cellulose acetate nanofibers (CAN) as a transformative scaffold for muscle tissue engineering (MTE), specifically in the context of cultivated meat applications. Through a comparative analysis between random and aligned CAN, utilizing C2C12 and H9c2 myoblasts, we unveil the unparalleled capabilities of random CAN in facilitating muscle differentiation, independent of differentiation media, by exploiti

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