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Relevant bibliographies by topics / Habitat manipulation and transplantation

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Academic literature on the topic 'Habitat manipulation and transplantation'

Author: Grafiati

Published: 4 June 2021

Last updated: 5 February 2022

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Journal articles on the topic "Habitat manipulation and transplantation"

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Nemoy, P., E. Spanier, N. Kashtan, A. Israel, and D. L. Angel. "Plasticity of marine sponge habitat preferences with regard to light and water motion: the example of Batzella inops (Topsent, 1891) in submerged caves." Marine and Freshwater Research 69, no. 11 (2018): 1784. http://dx.doi.org/10.1071/mf18001.

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This study examined the effects of environmental conditions on the distribution of marine sponges. We measured the abundance of the sponge Batzella inops (Topsent, 1891) in two contrasting habitats: inside submerged caves and on the surfaces of submerged boulders. We hypothesised that caves are a preferred habitat for B. inops over the boulder surfaces, and tested this by descriptive (quadrate sampling) and manipulative (reciprocal transplantation) experiments. In addition, we tested B. inops in situ for the presence of photosynthetic activity. We found that B. inops is more abundant inside the caves (mean ± s.e.m., 1.2 ± 0.6individualsm–2) than on the outside boulder surfaces (0.15 ± 0.19individualsm–2). We also detected photosynthetic activity in B. inops in both habitats. The results of transplantation experiments suggested that the sponge prefers the transfer from inside to outside the cave rather than vice versa. Therefore, we conclude that although B. inops is more abundant in sheltered habitats, such as submerged caves, adult individuals of this sponge can survive transfer to exposed conditions. Altogether, our findings point to the plasticity of B. inops habitat preferences and may aid further research into conservation or mariculture of this and possibly other sponge species.

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Atkinson, Kerry-Lynn, and Christian Lacroix. "Evaluating reintroduction methods for the Gulf of Saint Lawrence aster (Symphyotrichum laurentianum) on Prince Edward Island." Botany 91, no. 5 (May 2013): 293–99. http://dx.doi.org/10.1139/cjb-2012-0074.

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The Gulf of St. Lawrence aster (SLA; Symphyotrichum laurentianum (Fernald) G.L. Nesom) is an annual plant species endemic to the Gulf of St. Lawrence region. Owing to the dynamic nature of the environment that the SLA inhabits, severe and major threats to both the aster and its habitat exist. The Committee on the Status of Endangered Wildlife in Canada listed the species as threatened in Canada in 2004. This status was assigned based on the species' limited distribution, fluctuating population size, and continued pressures on its habitat. Surveys have revealed that both site and population numbers have been further and drastically reduced on Prince Edward Island. In 2007, only one populated site of 482 individuals remained. It is possible that this species has been extirpated from Prince Edward Island. Recovery of this species on Prince Edward Island is feasible. Promising results related to seeding and the transplantation of greenhouse-grown seedlings at four in situ sites demonstrated that SLA plantlets have the potential to serve as seed stock to re-establish populations. Over the 2 years of the transplantation experiment, the pooled overall survivorship was 52.8%. Specific site manipulations that were tested may also increase the potential survivorship of the transplants and facilitate second-generation germination.

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Putman, Breanna J., and Rulon W. Clark. "Habitat Manipulation in Hunting Rattlesnakes (CrotalusSpecies)." Southwestern Naturalist 60, no. 4 (December 2015): 374–77. http://dx.doi.org/10.1894/0038-4909-60.4.374.

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Sliva, Lucie, and D. Dudley Williams. "Responses of Hyporheic Meiofauna to Habitat Manipulation." Hydrobiologia 548, no. 1 (October 2005): 217–32. http://dx.doi.org/10.1007/s10750-005-5445-y.

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Borkakati, Rudra N., D. K. Saikia, and M. R. Venkatesh. "Habitat manipulation for managing insect pests of Brinjal." Indian Journal of Entomology 81, no. 4 (2019): 717. http://dx.doi.org/10.5958/0974-8172.2019.00184.6.

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Apollonio, Marco, Marco Festa-Bianchet, Franco Mari, Elisabetta Bruno, and Maurizio Locati. "Habitat Manipulation Modifies Lek Use in Fallow Deer." Ethology 104, no. 7 (April 26, 2010): 603–12. http://dx.doi.org/10.1111/j.1439-0310.1998.tb00095.x.

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Baine, M., and J. Side. "Habitat modification and manipulation as a management tool." Reviews in Fish Biology and Fisheries 13, no. 2 (2003): 187–99. http://dx.doi.org/10.1023/b:rfbf.0000019480.95010.67.

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Nickerson, Peter, J??rg Steiger, Xin Xiao Zheng, Alan W. Steele, Wolfgang Steurer, Prabir Roy-Chaudhury, and Terry B. Strom. "MANIPULATION OF CYTOKINE NETWORKS IN TRANSPLANTATION." Transplantation 63, no. 4 (February 1997): 489–94. http://dx.doi.org/10.1097/00007890-199702270-00001.

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Hossain, Z., G. M. Gurr, and S. D. Wratten. "Habitat manipulation for lucerne: a renaissance for strip-cutting?" Proceedings of the New Zealand Plant Protection Conference 50 (August 1, 1997): 545. http://dx.doi.org/10.30843/nzpp.1997.50.11394.

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Szendrei, Z., M. Kramer, and D. C. Weber. "Habitat manipulation in potato affects Colorado potato beetle dispersal." Journal of Applied Entomology 133, no. 9-10 (December 2009): 711–19. http://dx.doi.org/10.1111/j.1439-0418.2009.01429.x.

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Dissertations / Theses on the topic "Habitat manipulation and transplantation"

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Priddis, Edmund R. "Niche Separation Along Environmental Gradients as a Mechanism to Promote the Coexistence of Native and Invasive Species." Diss., CLICK HERE for online access, 2007. http://contentdm.lib.byu.edu/ETD/image/etd2199.pdf.

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Dellen, Anton van. "Cortical transplantation and manipulation in studies of dvelopment and disease." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.404275.

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Palermo, Gianpiero D. "Manipulation of development by nuclear transfer." Monash University, Institute of Reproduction and Development, 2004. http://arrow.monash.edu.au/hdl/1959.1/9693.

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Gok, Muhammed Asim. "Solid Organ Assessment and Manipulation for Transplantation from Non Heart Beating Donors." Thesis, University of Newcastle upon Tyne, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.484819.

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Whilst kidney transplantation is accepted as a cost effective treabnent ofend stage renal failure, the shortage in the kidney donor pool has recently become critical. Historical series ofNHBD renal transplants have been associated with high rates ofallograft failure and dysfunction due to prolonged warm and cold ischaemia. Machine preservation of NHBD kidneys provides a useful tool to test viability pre-transplant and this PhD thesis was directed at improving the existing Newcastle NHBD programme. Newcastle machine preservation is described in which viability criteria is used to reduced primary non function and discard rates. The sequential allograft failures were critically reviewed to develop the new 'IO-point' criteria. Case failures have become a necesSary painful learning experience that allowed improvements in the selection and screening criteria. The long term function ofNHBD renal transplants are presented to illustrate how the function is affected by warm ischaemic injury and delayed graft function. The early dysfunction ofNHBD kidney transplants was found to be temporary and improved with time. Kidney perfusate GST evaluation is an established criteria in the assessment of viability ofNHBD kidneys. In the search for novel biomarkers, perfusate Ala-AP and FABP have been assessed in relation to GST. The novel biomarkers measured a different aspect of allograft injury and comparable results to GST suggest that these could be developed as an adjunct to perfusate GST. The introduction ofstreptokinase pre-flush of the NHBD is illustrated in a porcine and a subsequent human clinical model. The effect on procurement, machine preservation and transplantation has been evaluated. Thrombolytic therapy ofNHB donor facilitated the clearing ofpremorbid intravascular thrombus, thereby improving the preservation and thus the viability of the NHBD kidneys. Allograft dysfunction is a standard feature of ischaemia reperfusion injury. A clinical model ofkidney transplantation is described to illustrate the biochemical and clinical effects of ischaemia reperfusion. An exaggerated response was observed in NHBD kidney transplants that could be attributed to the warm ischaemia insult at time of cardiac arrest.

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Begum, Mahmuda. "Habitat manipulation to enhance biological control of lightbrown apple moth (Epiphyas postvittana) /." Connect to full text, 2004. http://hdl.handle.net/2123/690.

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Tan, Peng Hong. "Genetic manipulation of endothelium and dendritic cells : potential strategies to induce transplantation tolerance." Thesis, Imperial College London, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.411403.

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Taghizadeh-Afshari, Fardad. "Manipulation of integrins to enhance the integration and migration of cells for transplantation." Thesis, University of Cambridge, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611822.

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Cohn, E. "The manipulation, introduction and ecology of field layer communities in broadleaved woodlands." Thesis, University of Wolverhampton, 1994. http://hdl.handle.net/2436/96288.

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Begum, Mahmuda. "Habitat manipulation to enhance biological control of light brown apple moth (Epiphyas Postvittana)." University of Sydney. Rural Management, 2004. http://hdl.handle.net/2123/690.

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Trichogramma carverae Oatman and Pinto is mass-released for biological control of the leafroller pest, light brown apple moth, Epiphyas postvittana (Walker) in Australian vineyards. Parasitoid performance can, however, be constrained by a lack of suitable adult food and no information is available on the effect of nectar on the parasitism and longevity of T. carverae. To address this, the effect of alyssum, Lobularia maritima (L.) flowers on E. postvittana parasitism was studied in a vineyard experiment with and without releases of T. carverae. Egg parasitoid activity was assessed with E. postvittana egg �sentinel cards� and no parasitism was recorded in plots without T. carverae releases. Where T. carverae were released, there was no significant enhancement of parasitism by the presence of L. maritima flowers. Three hypotheses were subsequently tested to account for the lack of an effect: (i) T. carverae does not benefit from L. maritima nectar, (ii) T. carverae was feeding on nectar from other flowering plants (weeds) present in the vineyard, (iii) T. carverae was feeding on sugars from ripe grapes. A growth-cabinet experiment using potted L. maritima plants with and without flowers did not support hypothesis one. No parasitism was recorded after day two for T. carverae caged without flowers whilst parasitism occured until day eight in the presence of flowers. A laboratory experiment with common vineyard weeds (Trifolium repens, Hypochoeris radicata, Echium plantagineum) as well as L. maritima did not support hypothesis one but gave partial support to hypothesis two. Survival of T. carverae was enhanced to a small but statistically significant extent in vials with intact flowers of L. maritima, white clover (T. repens) and catsear (H. radicata) but not in vials with flowering shoots of these species from which flowers and flowering buds had been removed. Paterson�s curse (E. plantagineum) flowers had no effect on T. carverae survival. In a laboratory study, punctured grapes significantly enhanced T. carverae survival compared with a treatment without grapes, supporting hypothesis three. Trichogramma carverae performance in the field experiment was probably also constrained by relatively cool and wet weather. Further work on the enhancement of T. carverae efficacy by L. maritima and other carbohydrate sources is warranted. Greenhouse and field experiments were conducted to investigate whether T. carverae benefit from different groundcover plant species. Ten T. carverae adults (<24h after eclosion) were caged with different groundcover species and a control with no plant materials. Epiphyas postvittana egg sentinel cards were used to measure parasitism and longevity was recorded visually. Survival and realised parasitism of T. carverae was significantly higher in L. maritima than in Brassica juncea, Coriandrum sativum, shoots of these species from which flowers had been removed and nil control treatments. A similar experiment with Fagopyrum esculentum (with- and without-flowers) and a control treatment showed that survival was significantly higher in intact F. esculentum than in without-flower and control treatments. There was no significant treatment effect on parasitism in the early stages of that experiment, though parasitism was recorded in the presence of F. esculentum flowers for 12 days, compared with 6 days in other treatments. Higher parasitism was observed in intact Borago officinalis than in the flowerless shoot, water only and no plant material control treatments in a third experiment. There was no significant treatment effect on parasitism. Fitted exponential curves for survival data differed significantly in curvature in the first, second and third experiments but the slope was a non-significant parameter in the second and third experiments. In a second series of laboratory experiments, one male and one female T. carverae were caged with groundcover species to investigate male and female longevity and daily fecundity. Both male and female longevity in F. esculentum and L. maritima treatments were significantly higher than on shoots of these species from which flowers had been removed, and than in the control treatments. Daily fecundity was significantly greater in the intact L. maritima treatment than in all other treatments. Fitted exponential curves for daily fecundity differed significantly in position and slope but not in curvature. There was no significant treatment effect on longevity or parasitism when a male and female were caged with intact B. juncea, B. officinalis or without-flower of these species, nor in the treatment with no plant materials. No parasitism was observed in a survey of naturally occurring egg parasitoids on two sites close to Orange and Canowindra in New South Wales, illustrating the importance of mass releases of T. carverae in biological control of E. postvittana. In an experiment on the Canowindra site, parasitism was significantly higher on day one and day two after T. carverae release when with-flower treatments were compared with without-flower treatments. Parasitism was significantly higher in the F. esculentum treatment than in C. sativum, L. maritima, vegetation without-flowers and control treatments on these dates. On day five, parasitism was higher in C. sativum than in all other treatments. There was no significant increase in parasitism in a second experiment conducted on the Orange site. Coriandrum sativum, F. esculentum and L. maritima appear to be suitable adult food sources for T. carverae and offer some scope for habitat manipulation in vineyards The adults of many parasitoid species require nectar for optimal fitness but very little is known about flower recognition. Flight cage experiments showed that the adults of T. carverae benefited from L. maritima bearing white flowers to a greater extent than was the case for light pink, dark pink or purple flowered cultivars, despite all cultivars producing nectar. Survival and realised parasitism on non-white flowers were no greater than when the parasitoids were caged on L. maritima shoots from which flowers had been removed. The possibility that differences between L. maritima cultivars were due to factors other than flower colour, such as nectar quality, was excluded by dyeing white L. maritima flowers by placing the roots of the plants in 5% food dye (blue or pink) solution. Survival of T. carverae was lower on dyed L. maritima flowers than on undyed white flowers. Mixing the same dyes with honey in a third experiment conducted in the dark showed that the low level of feeding on dyed flowers was unlikely to be the result of olfactory or gustatory cues. Flower colour appears, therefore, to be a critical factor in the choice of plants used to enhance biological control, and is likely to also be a factor in the role parasitoids play in structuring invertebrate communities. Provision of nectar producing plants to increase the effectiveness of biological control is one aspect of habitat manipulation, but care needs to be taken to avoid the use of plant species that may benefit pest species. Greenhouse experiments were conducted to investigate whether the adult E. postvittana and larvae benefit from nectar producing groundcover species. Newly emerged E. postvittana adults were caged with different groundcover species and a honey-based artificial adult diet. The longevity of male and female E. postvittana when caged with shoots of borage (B. officinalis) and buckwheat (F. esculentum) bearing flowers was as long as when fed a honey-based artificial diet. This effect was not evident when caged with shoots of these plants from which flowers had been removed. Longevity was significantly lower than in the artificial diet treatment when caged with coriander (C. sativum) or alyssum (L. maritima) irrespective of whether flowers were present or not. There was no significant treatment effect on the lifetime fecundity of E. postvittana. A second experiment with mustard (B. juncea) (with- and without-flowers), water only and honey-based artificial adult diet showed no significant treatment effects on the longevity of male and female E. postvittana or on the lifetime fecundity of E. postvittana. The anomalous lack of a difference between the water and honey-based diet treatments precludes making conclusions on the value of B. juncea for E. postvittana. Two greenhouse experiments were conducted to evaluate the effects of groundcover species on the larval development of E. postvittana. In the first experiment, larval mortality was significantly higher in C. sativum, and L. maritima than in B. juncea, B. officinalis and white clover (T. repens) a known host of E. postvittana. Coriandrum sativum and L. maritima extended the larval period. In B. juncea and B. officinalis, mortality did not differ from that in T. repens. In F. esculentum, larval mortality was significantly higher than in T. repens. A short larval period was observed on B. juncea, B. officinalis and F. esculentum. Fitted exponential curves for larval mortality differed significantly in curvature between plant treatments. Similarly, successful pupation was significantly lower in C. sativum, F. esculentum and L. maritima than in T. repens. The percentage of successful pupation in B. juncea and B. officinalis did not differ from F. esculentum and T. repens. Fitted exponential curves for pupation differed significantly in curvature. A similar trend was observed in a second experiment with potted plants. The overall results suggest that C. sativum and L. maritima denied benefit to E. postvittana adults and larvae, so could be planted as vineyard groundcover with minimal risk of exacerbating this pest. Overall results suggest that T. carverae require nutrients to reach their full reproductive potential and flowers provide such nutrients. Lobularia maritima and C. sativum may be considered �selective food plants� for T. carverae whereas F. esculentum appears to be a �non-selective food plant�; both T. carverae and E. postvittana benefited from it. Fruits such as grapes can be used as food resources in habitat manipulation and this merits further research. This result also suggests that within species flower colour is an important factor for flower selection in habitat manipulation.

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Hickman, Janice Mary. "The usefulness of Phacelia tanacetifolia strips as a resource for aphidophagous hoverflies (Diptera: syrphidae) on arable land." Thesis, University of Southampton, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241989.

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Books on the topic "Habitat manipulation and transplantation"

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Frezza, T. Assessing fish habitat supply and potential responses to habitat manipulation in small Canadian Shield lakes. Burlington, Ont: Fisheries and Oceans Canada, Great Lakes Laboratory for Fisheries and Aquatic Sciences, 2002.

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Frezza, T. Assessing fish habitat supply and potential responses to habitat manipulation in small Canadian Shield lakes. [Ottawa?]: Fisheries and Oceans, 2002.

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Gowan, Charles. Trout responses to habitat manipulation in streams at individual and population scales. Fort Collins, Colo: Colorado Divison of Wildlife, Fish Research Section, 1995.

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Gurr, G. M., S. D. Wratten, and M. A. Altieri, eds. Ecological engineering for pest management: advances in habitat manipulation for arthropods. Wallingford: CABI, 2003. http://dx.doi.org/10.1079/9780851999036.0000.

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Kantrud, Harold A. Effects of vegetation manipulation on breeding waterfowl in prairie wetlands: A literature review. Washington, D.C: United States Dept. of the Interior, Fish and Wildlife Service, 1986.

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Società italiana di embryo transfer. Congress. Embryonic development and manipulation in animal production. Edited by Lauria A, Gandolfi F, Biochemical Society (Great Britain), and International Symposium on Embryonic Technology in Domestic Species (1992 : Milan, Italy). London: Portland Press on behalf of the Biochemical Society, 1992.

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A, Lauria, Gandolfi F, and International Symposium on Embryonic Technology in Domestic Species (1992 : Milan, Italy), eds. Embryonic development and manipulation in animal production: Trends in research and applications : proceedings of the 1st Congress of the Italian Society of Embryo Transfer and the International Symposium on Embyonic Technology in Domestic Species, Milan. London: Portland Press, 1992.

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Gurr, Geoff. Ecological Engineering for Pest Management: Advances in Habitat Manipulation for Arthropods. CSIRO Publishing, 2004.

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Geoff, Gurr, Wratten Stephen D, and Altieri Miguel A, eds. Ecological engineering for pest management: Advances in habitat manipulation for arthropods. Ithaca, N.Y: Comstock Pub. Associates, 2004.

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Ecological Engineering For Pest Management: Advances In Habitat Manipulation For Arthropods. Cornell University Press, 2004.

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Book chapters on the topic "Habitat manipulation and transplantation"

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Cho, Christina, and Miguel-Angel Perales. "Graft manipulation." In Clinical Manual of Blood and Bone Marrow Transplantation, 66–72. Chichester, UK: John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781119095491.ch8.

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Lees, Jason R., Agnes M. Azimzadeh, Yaozhong Ding, Jiangnan Xu, and Jonathan S. Bromberg. "Current Pathways for Immune Manipulation." In Textbook of Organ Transplantation, 219–38. Oxford, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118873434.ch17.

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Webber, Allison B., and Flavio Vincenti. "Future Pathways for Immune Manipulation." In Textbook of Organ Transplantation, 239–49. Oxford, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118873434.ch18.

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Russell, L. D., M. Nagano, and R. L. Brinster. "Spermatogonial Transplantation." In Testicular Function: From Gene Expression to Genetic Manipulation, 41–57. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-662-03671-6_3.

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Adair, Jennifer E., Grant D. Trobridge, and Hans-Peter Kiem. "Genetic Manipulation of Hematopoietic Stem Cells." In Thomas’ Hematopoietic Cell Transplantation, 78–99. Chichester, UK: John Wiley & Sons, Ltd, 2016. http://dx.doi.org/10.1002/9781118416426.ch8.

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Bhat, Sunil, and Arun Singh Danewa. "Ex Vivo Manipulation of Stem Cell Product." In Contemporary Bone Marrow Transplantation, 1–16. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-319-64938-2_15-1.

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Bhat, Sunil, and Arun Singh Danewa. "Ex Vivo Manipulation of Stem Cell Product." In Contemporary Bone Marrow Transplantation, 483–98. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-36358-1_15.

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Ho, Vincent T. "Graft Manipulation: T Cell Depletion and Tumor Cell Purging." In Hematopoietic Stem Cell Transplantation, 653–86. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-438-4_27.

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Schlatt, S., A. G. Schepers, and V. Schönfeldt. "Germ Cell Culture, Genetic Manipulation and Transplantation." In Testis, Epididymis and Technologies in the Year 2000, 69–86. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-662-04050-8_6.

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Van Zant, G., and H. J. Deeg. "Genetic Manipulation of Hematopoietic Stem Cells." In A Guide to Blood and Marrow Transplantation, 87–91. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-18248-8_10.

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Conference papers on the topic "Habitat manipulation and transplantation"

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Kimura, Y., M. Gel, B. Techaumnat, O. Kurosawa, K. Tsuda, H. Oana, H. Kotera, T. Tada, and M. Washizu. "A Novel Cell Manipulation Device for Cytoplasmic Transplantation." In 2008 International Symposium on Micro-NanoMechatronics and Human Science (MHS). IEEE, 2008. http://dx.doi.org/10.1109/mhs.2008.4752449.

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Wang, Xuefeng, Na Li, Yaowei Liu, Mingzhu Sun, and Xin Zhao. "Pipelined batch-operation process of nuclear transplantation based on micro-manipulation system." In 2016 IEEE International Conference on Robotics and Biomimetics (ROBIO). IEEE, 2016. http://dx.doi.org/10.1109/robio.2016.7866518.

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Tongnunui, Prasert, Prasert Tongnunui, Woraporn Tarangkoon, Woraporn Tarangkoon, Parichat Hukiew, Parichat Hukiew, Patcharee Kaeoprakan, et al. "SEAGRASS RESTORATION: AN UPDATE FROM TRANG PROVINCE, SOUTHWESTERN THAILAND." In Managing risks to coastal regions and communities in a changing world. Academus Publishing, 2017. http://dx.doi.org/10.21610/conferencearticle_58b431687e149.

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Natural disasters may adversely affect coastal resources potentially leading to coastal habitat restorations that incorporate stakeholders and the general public. Appropriate methodologies for habitat restoration are developed to ensure the outcomes of this project. Currently, seagrass bed restoration by means of asexual and sexual propagation techniques have been used worldwide. However, the experience of seagrass (Enhalus acoroides) habitat restoration in Trang Province noted that to accomplish this project’s strategies involved the application of restoration techniques along with public and stakeholder participation. The application of asexual propagation, specifically the collection of single shoots from donor seagrasses and subsequent transplantation, is a convenient tool. However, from this project results, this process still has conceptual problems as from the large numbers of single shoots collected from donor seagrasses, the survival rate was relatively low. Furthermore, this process was complicated by conflicting interests between local communities near to the donor site and the project’s organizers. In order to reduce said conflicts, other techniques to balance stakeholder interests were instigated by this project, namely the development of both asexual and sexual propagation techniques. This project initiated a sexual propagation technique by the collection of wild seeds of Enhalus acoroides that were subsequently grown in the laboratory before natural habitat transplantation. This project results showed that seeds can be grown rapidly and can be cultured in large numbers. However, this development technique has a limit on rearing time because seedlings were found to be in decline after the third month of the experiment. These problems were compounded by a limiting factor that pushed the project’s organizers to decide to transplant seagrasses from the laboratory to the wild whether a time was seasonally suitable or unsuitable, the planting activity still done forward. This matter may have enhanced the low survival rate situation after seagrass transplantation to the wild. If there is a need to recover a seagrass bed, the above culture and transplantation methodologies should be used in conjunction with repeated periodic plantings until natural ecological function has been restored. In conclusion, further research should be instigated to improve the cultivation method for producing ready to plant seedlings and to improve methods of project operation.

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Tongnunui, Prasert, Prasert Tongnunui, Woraporn Tarangkoon, Woraporn Tarangkoon, Parichat Hukiew, Parichat Hukiew, Patcharee Kaeoprakan, et al. "SEAGRASS RESTORATION: AN UPDATE FROM TRANG PROVINCE, SOUTHWESTERN THAILAND." In Managing risks to coastal regions and communities in a changing world. Academus Publishing, 2017. http://dx.doi.org/10.31519/conferencearticle_5b1b9447ad58f1.23030316.

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Natural disasters may adversely affect coastal resources potentially leading to coastal habitat restorations that incorporate stakeholders and the general public. Appropriate methodologies for habitat restoration are developed to ensure the outcomes of this project. Currently, seagrass bed restoration by means of asexual and sexual propagation techniques have been used worldwide. However, the experience of seagrass (Enhalus acoroides) habitat restoration in Trang Province noted that to accomplish this project’s strategies involved the application of restoration techniques along with public and stakeholder participation. The application of asexual propagation, specifically the collection of single shoots from donor seagrasses and subsequent transplantation, is a convenient tool. However, from this project results, this process still has conceptual problems as from the large numbers of single shoots collected from donor seagrasses, the survival rate was relatively low. Furthermore, this process was complicated by conflicting interests between local communities near to the donor site and the project’s organizers. In order to reduce said conflicts, other techniques to balance stakeholder interests were instigated by this project, namely the development of both asexual and sexual propagation techniques. This project initiated a sexual propagation technique by the collection of wild seeds of Enhalus acoroides that were subsequently grown in the laboratory before natural habitat transplantation. This project results showed that seeds can be grown rapidly and can be cultured in large numbers. However, this development technique has a limit on rearing time because seedlings were found to be in decline after the third month of the experiment. These problems were compounded by a limiting factor that pushed the project’s organizers to decide to transplant seagrasses from the laboratory to the wild whether a time was seasonally suitable or unsuitable, the planting activity still done forward. This matter may have enhanced the low survival rate situation after seagrass transplantation to the wild. If there is a need to recover a seagrass bed, the above culture and transplantation methodologies should be used in conjunction with repeated periodic plantings until natural ecological function has been restored. In conclusion, further research should be instigated to improve the cultivation method for producing ready to plant seedlings and to improve methods of project operation.

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Zhou, Robin, Andrew Hughes, Jane L. Liesveld, and Michael R. King. "Nanoparticle-Coated Microtubes for the Manipulation of Cancer Cells." In ASME 2010 8th International Conference on Nanochannels, Microchannels, and Minichannels collocated with 3rd Joint US-European Fluids Engineering Summer Meeting. ASMEDC, 2010. http://dx.doi.org/10.1115/fedsm-icnmm2010-30168.

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The development of novel methods for the isolation of primary stem and progenitor cells is important for the treatment of blood cancers, tissue engineering, and basic research in the biomedical sciences. Our lab has previously shown that microtubes coated with P-selectin protein can be used to capture and enrich hematopoietic stem and progenitor cells from a mixture of cells perfused through the tube at physiologically-relevant shear stresses[1][2], and that using a surface coating of colloidal silica nanoparticles (12 nm diameter, 30% by weight SiO2) increased cell capture and decreased rolling velocity[3]. Here we show that 50 nm colloidal silica nanoparticle coatings may similarly increase cell capture, and that these protocols are effective for enrichment of human adult CD34-positive HSCs from primary apheresis and bone marrow aspirate samples. Future research may include long-term colony-forming assays to confirm stem cell activity of enriched cells, and transplantation in immune-deficient mice.

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Elfeky, R., RM Shah, MNM Unni, K. Rao, R. Chiesa, P. Amrolia, A. Worth, et al. "132 Improved survival and outcome of HLA-mismatched donor hematopoietic stem cell transplantation in children with primary immunodeficiencies using new graft manipulation strategies in the UK." In Great Ormond Street Hospital Conference 2018: Continuous Care. BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health, 2018. http://dx.doi.org/10.1136/goshabs.132.

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Yoshimori, Takashi, Masaki Fukagawa, and Hiroshi Takamatsu. "Effect of Cell-to-Surface Interaction on Freeze Tolerance and Osmotic Response of Cells." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192404.

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Cryopreservation of tissues and organs, including artificial organs, could be one of the important steps in the medical service that brings the progress in the tissue engineering to realization. In this case, high viability of cryopreserved cells is critical to recovery after transplantation. In contrast, in the cryosurgery, which is expected to expand its application as a minimally invasive treatment of cancer, malignant cells should be destructed completely to prevent from recurrence. The appropriate freeze-thaw protocol is therefore needed to be established for cryopreservation or cryosurgery depending on specific type of tissues and organs. Although it is determined empirically, the underlying mechanism of cell injury by freezing has been explored for a long time to give a scientific basis of the process. The experiments with a cell suspension showed that the cell injury during slow freezing to a relatively higher sub-zero temperature was attributed to the mechanical stress from the extracellular ice, while the effect of elevated concentration of solutes became more crucial to cell damage at lower temperatures [1]. However, there are some studies that indicates the difference in the freeze tolerance between cell suspensions and attached monolayers, some of which indicated higher susceptibility of monolayers to freezing than cell suspension [2] and the other suggested reverse [3,4]. The goal of our study is thus to validate the difference in freezing injury between isolated cells and tissues that are more important in aforementioned applications and clarify the mechanism. We used cells adhered to a surface as a first simple model of cells in tissues. The cells adhered on a surface at low number density were used to highlight the effect of cell-to-surface interaction without cell-to-cell interactions. In the present study we first demonstrate that the survival of cells adhered on a surface is lower than those in the suspension after a freeze-thaw manipulation. Then the osmotic response to concentration increase was examined to clarify if the extent of dehydration is different between these two types of cells. The cells were observed by a laser confocal scanning microscope that allows real-time 3-D observation.

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