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1

Egan, Siobhon L., Manuel Ruiz-Aravena, Jill M. Austen, Xavier Barton, Sebastien Comte, David G. Hamilton, Rodrigo K. Hamede, et al. "Blood Parasites in Endangered Wildlife-Trypanosomes Discovered during a Survey of Haemoprotozoa from the Tasmanian Devil." Pathogens 9, no. 11 (October 23, 2020): 873. http://dx.doi.org/10.3390/pathogens9110873.

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The impact of emerging infectious diseases is increasingly recognised as a major threat to wildlife. Wild populations of the endangered Tasmanian devil, Sarcophilus harrisii, are experiencing devastating losses from a novel transmissible cancer, devil facial tumour disease (DFTD); however, despite the rapid decline of this species, there is currently no information on the presence of haemoprotozoan parasites. In the present study, 95 Tasmanian devil blood samples were collected from four populations in Tasmania, Australia, which underwent molecular screening to detect four major groups of haemoprotozoa: (i) trypanosomes, (ii) piroplasms, (iii) Hepatozoon, and (iv) haemosporidia. Sequence results revealed Trypanosoma infections in 32/95 individuals. Trypanosoma copemani was identified in 10 Tasmanian devils from three sites and a second Trypanosoma sp. was identified in 22 individuals that were grouped within the poorly described T. cyclops clade. A single blood sample was positive for Babesia sp., which most closely matched Babesia lohae. No other blood protozoan parasite DNA was detected. This study provides the first insight into haemoprotozoa from the Tasmanian devil and the first identification of Trypanosoma and Babesia in this carnivorous marsupial.
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2

Rekha, B., G. Ponnudurai, and N. Rani. "Detection of haemoprotozoan parasites of sheep in ticks." Indian Journal of Small Ruminants (The) 27, no. 1 (2021): 135–38. http://dx.doi.org/10.5958/0973-9718.2021.00019.2.

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3

Velusamy, R., N. Rani, G. Ponnudurai, and P. Anbarasi. "Prevalence of intestinal and haemoprotozoan parasites of small ruminants in Tamil Nadu, India." Veterinary World 8, no. 10 (October 2015): 1205–9. http://dx.doi.org/10.14202/vetworld.2015.1205-1209.

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4

Aktas, Munir, and Sezayi Ozubek. "A survey of canine haemoprotozoan parasites from Turkey, including molecular evidence of an unnamed Babesia." Comparative Immunology, Microbiology and Infectious Diseases 52 (June 2017): 36–42. http://dx.doi.org/10.1016/j.cimid.2017.05.007.

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5

Holmes, P. H. "Pathophysiology of parasitic infections." Parasitology 94, S1 (January 1987): S29—S51. http://dx.doi.org/10.1017/s0031182000085814.

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SUMMARYParasites can have a wide range of pathophysiological effects on the host. This review describes those associated with some parasites of major importance in man and animals. Haemoprotozoan diseases such as trypanosomiasis and malaria are primarily associated with anaemia. Such anaemias have a complex aetiology involving various mechanisms responsible for red cell destruction as well as possible defects in red cell production. In addition to these haematological effects these diseases are associated with marked disturbances in heart function and the nervous, immune and urinary systems. The other major groups of parasitic diseases are those associated with the gastrointestinal tract. The most advanced studies have been conducted on the pathophysiology of gastrointestinal nematode parasites of sheep and have revealed significant effects on feed intake, gastrointestinal function, and protein and energy metabolism. Similar studies have yet to be conducted in other hosts and parasitic diseases. There is also a need to examine in greater detail the factors which can modulate pathophysiological responses by the host to parasitic infections.
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M’ghirbi, Youmna, Amaia Ros-García, Pilar Iribar, Adel Rhaim, Ana Hurtado, and Ali Bouattour. "A molecular study of tick-borne haemoprotozoan parasites (Theileria and Babesia) in small ruminants in Northern Tunisia." Veterinary Parasitology 198, no. 1-2 (November 2013): 72–77. http://dx.doi.org/10.1016/j.vetpar.2013.08.005.

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7

Opara, M. N., T. S. Adewumi, B. R. Mohammed, S. S. Obeta, M. K. Simon, O. C. Jegede, and R. I. S. Agbede. "Investigations on the Haemoprotozoan Parasites of Nigerian Local Breed of Dogs in Gwagwalada Federal Capital Territory (FCT) Nigeria." Research Journal of Parasitology 12, no. 1 (December 15, 2016): 1–7. http://dx.doi.org/10.3923/jp.2017.1.7.

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8

Mehta, H. K., Mahima, ., RK Bagherwal, and R. Chaurasia. "Clinical and Ultrasonographic Alterations in Canine Babesiosis." INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY 15, no. 03 (March 9, 2020): 30–31. http://dx.doi.org/10.21887/ijvsbt.15.3.8.

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Canine babesiosis is a worldwide tick borne disease. Dogs with fever, in appetence and enlarged lymph nodes were screened for the presence of haemoprotozoan parasites during the one year period of study at T.V.C.C., C.V.Sc., Mhow (M.P. , India ). Based on the stained peripheral blood smears examination, dogs were found to be affected with babesiosis. Clinical examination of the dogs revealed ticks over the body, highest frequency (75.00%) of petechial/epistaxis followed by ticks (72.22%) on the body, recumbency (69.44%), dullness (66.66%), dehydration (63.88%), pale mucous membrane (50.00%) . Sonographic changes in dogs infected with babesiosis revealed hepatomegaly with architectural changes, hypo-echoic changes in the liver parenchyma in 7 dogs, splenomegaly with disturbed architecture and multiple lesions was observed in 9 dogs, cystitis in 5 dogs , distended gall bladder in 3 dogs and in 4 dogs Kidneys structures were not clear in image and no demarcation noticed between cortex and medulla.
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9

Ogbaje, C. I., O. C. Nwosu, and C. D. Onah. "Modification of giemsa stain technique for better diagnosis of haemoprotozoan parasites and prevalence of bovine babesiosis in Makurdi Metropolis Major Abattoir." Nigerian Journal of Parasitology 40, no. 2 (October 1, 2019): 272. http://dx.doi.org/10.4314/njpar.v40i2.25.

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10

Gebisa, Gelaye, Kibiru Beriso, Biruk Bogale, Oda Gizaw, and Dawit Chala. "Bovine Trypanosomosis and Its Vectors in Three Selected Districts of Buno Bedele Zone of Oromia Region, Ethiopia." Veterinary Medicine International 2020 (July 25, 2020): 1–8. http://dx.doi.org/10.1155/2020/1571947.

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Trypanosomosis is one of the most economically challenging diseases affecting mammals, and it is a serious haemoprotozoan disease caused by different species of unicellular eukaryotic parasite of the genus trypanosome. The study was conducted to access the prevalence of bovine trypanosomosis, its associated risk factors, and vector density on cattle reared in three selected districts, namely, Chewaka, Dabo Hana, and Meko districts. Blood was collected from a total of 1046 cattle of age groups extending from 1 to 6 years. The buffy coat technique was used to check the presence of parasites from sampled blood, and the trypanosome species were identified using Giemsa-stained thin blood films. The packed cell volume of sampled blood was determined using the haematocrit. A total of 160 traps were deployed to study the entomological survey. Generally, 3.44% of the studied animal was infected with trypanosomosis, and T. vivax was the dominant species of trypanosomosis in the study areas. Significant differences (P < 0.05) were observed due to associated factor viz. body condition and anaemic status of the animal; however, insignificant differences were also recorded between different districts, age group, and sex. The mean PCV value of parasitaemic and aparasitaemic animals was 22.22 ± 0.92 and 26.18 ± 0.16, respectively, and significant difference was P < 0.05. An overall of 1.82 flies per trap per day was recorded from the study areas, and among the total caught vectors, 81.4% of it was G. tachinoides and the rest was G. morsitans. Therefore, the veterinarians have to continue providing the appropriate medication/treatment for the infected animals per appropriate recommendation, and Bedele NTTICC has to take more measures to control the density and distribution of tsetse flies in Dabo Hana district than the others due to high flies per trap per day observed in Dabo Hana district.
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11

Winiarczyk, Dagmara, Michał Bartnicki, Beata Abramowicz, Paweł Łyp, Jacek Madany, Stanisław Winiarczyk, and Łukasz Adaszek. "Utility of urinary markers in the assessment of renal dysfunction in canine babesiosis." Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere 45, no. 02 (2017): 84–88. http://dx.doi.org/10.15654/tpk-160490.

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SummaryObjective: Canine babesiosis is a common and clinically significant tick-borne disease caused by haemoprotozoan parasites of the genus Babesia. Acute renal failure is considered to be one of the most prevalent complications of canine babesiosis. This complication leads to a decrease in the glomerular filtration rate and in consequence causes azotemia and uremia. The objective of this study was to assess the localization and extent of renal damage in dogs infected with Babesia canis using an urinary marker for glomerular (urinary immunoglobulin G, uIgG), proximal tubular dysfunction (urinary retinol binding protein, uRBP) and distal tubular dysfunction (urinary Tamm-Horsfal protein, uTHP). Material und methods: In 10 dogs naturally infected with B. canis and 10 healthy control dogs the levels of urinary biomarkers were measured using commercially available ELISA tests. Results: Higher concentrations of uIgG, uRBP and uTHP were found in the urine of all dogs with babesiosis than in those from the control group. This indicates that in the course of the disease, the glomeruli as well as the renal tubules become damaged. Conclusion and clinical relevance: The study results allow a better understanding of the pathogenesis of canine babesiosis. However, in order to fully determine the extent and the nature of the damage to the kidneys of the infected dogs, it is advisable to conduct additional histopathological examinations of these organs.
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12

Adisu, Alemitu. "Study on Bovine Trypanosomosis and Associated Risk Factors in Chora District of Oromia Region, Ethiopia." Clinical Research and Clinical Trials 2, no. 3 (November 15, 2020): 01–06. http://dx.doi.org/10.31579/2693-4779/016.

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Bovine Trypanosomosis is one of the most parsimoniously challenging diseases in influencing mammals like cattle and it is a grave haemoprotozoan disease triggered by different species of unicellular eukaryotic parasite of the genus trypanosome. This study was conducted to assess the prevalence of bovine trypanosomosis, and the influence of associated risk factors on cattle reared in Chora district of Buno Bedele Zone. A Blood sample was collected from a total of 401 cattle of age ranges from 1 to 6 years. The presence of parasites from sampled blood was examined using a method of Buffy Coat Technique and a technique of Giemsa-stained thin blood films was used to identify the existing trypanosome species in the study area. The Packed Cell Volume (PCV) of collected blood samples was determined using haematocrit. A total of 50 traps were deployed to study the entomological survey. In general, 2.99 % of the studied cattle were infected with Trypanosomosis and the T.Vivax (58.33%) was the dominant species of trypanosomosis and followed by T. congulense (41.67%) in the study area. Statistically significant differences (P<0.05) were observed due to associated risk factors viz. Body Condition and age of cattle, whereas sampling sites and sex did not significantly affect the prevalence of Bovine Trypanosomosis. The mean PCV value of Parasitaemic and Aparasitaemic animals were 21.37 ± 0.19 and 27.89 ± 0.16, respectively and they were statistically different at (P< 0.05). Overall of 0.80 flies per trap per day was recorded from the study area and among the caught flies of Glossina species, 10%, 54% and 36% of them were, G.tachinoides, G.pallidipes and G.fuscipes, respectively. Therefore, the veterinarian’s has to continue providing the appropriate medication/treatment for the infected animals per appropriate recommendation and Bedele NTTICC has to take more focus to control the density and distribution of tsetse flies as well as biting flies for the transmission of high incidence of T. Vivax through mechanical ways of transmission of the disease.
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13

Sunday Idoko, Idoko, Sharon Tirosh-Levy, Monica Leszkowicz Mazuz, Babagana Mohammed Adam, Bello Sikiti Garba, Daniel Wesley Nafarnda, and Amir Steinman. "Genetic Characterization of Piroplasms in Donkeys and Horses from Nigeria." Animals 10, no. 2 (February 18, 2020): 324. http://dx.doi.org/10.3390/ani10020324.

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Equine piroplasmosis (EP) is a tick-borne disease of equids, caused by the two haemoprotozoal parasites: Theileria equi and Babesia caballi. Nigeria constitutes a major crossroads of animal transport in West Africa and may serve as a factor in EP dissemination in the region. The study aim was to characterize EP parasites in donkeys and horses in northern Nigeria using a molecular approach. Blood was collected from 57 donkeys and 47 horses. EP infection was detected and characterized by polymerase chain reaction (PCR). Twenty five donkeys (43.8%) were infected with T. equi, five (8.8%) with B. caballi, three (5.3%) with dual infections. Four horses (8.5%) were infected by T. equi and none by B. caballi. Four of the five known T. equi 18S rRNA genotypes (A, B, C and D) were identified. Theileria equi ema-1 and ema-2 genes were amplified in only 2 and 10 samples, respectively, showing no genetic variation. All B. caballi isolates were classified as rap-1 genotype A1. Twenty-two (42.3%) of the donkeys were positive for anti-T. equi antibodies and 29 (55.8%) were positive for anti-B. caballi antibodies, using immunofluorescence antibody test (IFAT). The study results demonstrate high genetic variation within T. equi parasites, suggesting that donkeys may be reservoirs of EP parasites in West Africa.
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14

Jenkins, Cheryl. "Bovine theileriosis in Australia: a decade of disease." Microbiology Australia 39, no. 4 (2018): 215. http://dx.doi.org/10.1071/ma18067.

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Theileriosis refers to the clinical disease caused by organisms from the genus Theileria, tick-borne haemoprotozoans infecting a diverse range of mammalian hosts. In Australia, Theileria spp. have been identified in both domestic and wildlife species but the bovine parasite, Theileria orientalis, has received the most attention due to the emergence and spread of clinical disease over the past 12 years, particularly in cattle herds on the east coast. At an estimated $20million per annum, the burden to cattle production is significant but despite over a decade of disease, there are still no effective chemotherapeutic treatments or vaccines available in Australia. Recent insights from genome sequencing studies reveal species level diversity within T. orientalis, which may help direct efforts at disease control.
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15

Famuyide, I. M., M. I. Takeet, A. O. Talabi, and E. B. Otesile. "Molecular Detection and Identification of Piroplasms in Semi-Intensively Managed Cattle from Abeokuta, Nigeria." Folia Veterinaria 64, no. 4 (December 1, 2020): 1–8. http://dx.doi.org/10.2478/fv-2020-0031.

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AbstractPiroplasmosis is a tick-borne haemolytic disease caused by different species of the Babesia and Theileria genera. Data on the prevalence of bovine piroplasms and their genetic diversity are scanty in Nigeria. Hence, this study reported the detection of some piroplasms in the blood of cattle in Abeokuta, Nigeria by the polymerase chain reaction (PCR). Blood samples were collected from 252 cattle and subjected to DNA extraction followed by PCR amplification of the partial region of 18S rRNA of the haemoprotozoans. Selected positive amplicons were unidirectionally sequenced and compared to the reference sequences from the Genbank. A total of 220 (87.3 %) cattle were positive for Theileria velifera and/or Babesia bigemina. The T. velifera was detected only in 163 (64.7 %) cattle, while 7 (2.8 %) cattle had a single infection with B. bigemina. Fifty cattle (19.8 %) had mixed infections with both parasites. There were no significant differences in piroplasm infections between the ages of cattle for both parasites. There were no significant differences in infection rates between the sexes for T. velifera, while the males had a significantly higher (P < 0.05) rate of infection for B. bigemina than the female cattle. The molecular detection of Babesia and Theileria species of cattle are reported for the first time in cattle in Abeokuta, Nigeria. This study, which confirmed the endemic nature of the parasites in cattle in the study area, stresses their importance in livestock health and production in Nigeria.
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16

OKUBO, K., N. YOKOYAMA, N. TAKABATAKE, M. OKAMURA, and I. IGARASHI. "Amount of cholesterol in host membrane affects erythrocyte invasion and replication by Babesia bovis." Parasitology 134, no. 5 (May 2006): 625–30. http://dx.doi.org/10.1017/s0031182006001910.

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SUMMARYCholesterol is a major component of the erythrocyte membrane. In the present study, we investigated the effects of cholesterol reduction in host bovine erythrocytes (RBC) on the growth of Babesia bovis, a major bovine haemoprotozoon. An in vitro growth assay with bovine RBC that had been prepared by pre-treatment with a cholesterol depletion agent (methyl-β-cyclodextrin, MCD) showed that the culture with 5 mm MCD-treated RBC inhibited the growth of B. bovis significantly as compared with that with the control RBC. In further experiments, the treatment with 5 mm MCD was proved to suppress both activities of the parasite, erythrocyte invasion and replication within the infected RBC. In contrast, a slight reduction in the membrane cholesterol by 1 mm MCD treatment promoted both their growth and erythrocyte invasion activity. These results indicate that erythrocyte invasion and replication by B. bovis are affected by the amount of cholesterol in the host erythrocyte membrane.
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17

Idowu, Emmanuel T., Oluwayomi O. Adeyemi, tephen C. Ezenwanne, Olubunmi A. Otubanjo, and Morakinyo B. Ajayi. "Eimeria Species and Haemosporidia of Domestic Chickens and Guinea Fowls Sold at Selected Poultry Markets in Lagos, Nigeria." Pan African Journal of Life Sciences 2, no. 1 (June 1, 2019): 67–72. http://dx.doi.org/10.36108/pajols/9102/20(0120).

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Introduction: Parasitic diseases are a major setback to sustainable poultry production. This study determined the occurrence of Eimeria species and haemosporidia among domestic chickens and helmeted guinea fowls in live-bird markets in Lagos State, Nigeria. Methods: Blood samples and intestinal contents at three distinct segments of the gut were collected from 60 domestic chickens and guinea fowls each. Wet smears of intestinal contents were microscopically examined for oocysts typical of Eimeria species while thin films of blood were Giemsa-stained for the demonstration of protozoa. Results: Results revealed that 19(31.7%) and 21(35%) chickens and guinea fowls w er e positive for Eimeria spp. infection respectively. There was no significant difference (P>0.05) in Eimeria spp. infections between the chicken breeds nor between sexes of both birds. Oocysts of Eimeria were mostly recovered from the caeca and small intestines of the guinea fowls and chickens respectively. Domestic chickens were infected with three different haemoprotozoa: Plasmodium spp. (23.3%), Leucocytozoon spp. (6.7%) and Haemoproteus spp. (3.3%); while Plasmodium spp. (15%) and Haemoproteus spp. (3.3%) were the only blood protozoa infecting guinea fowls. The infection rates of haemosporidia between the breeds of chickens and the sexes of both birds did not differ significantly (P>0.05). Conclusion: Eimeria parasites and haemosporidia are prevalent among chickens and guinea fowls sold in Lagos State. To prevent severe economic losses in the future, appropriate control measures should be designed and implemented.
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18

Madruga, Claudio R., Cássia R. B. Leal, Alda M. T. Ferreira, Flábio R. Araújo, Ana L. V. Bonato, Raul H. Kessler, Maria A. M. Schenk, and Cleber O. Soares. "Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regions of Brazil." Pesquisa Veterinária Brasileira 22, no. 4 (October 2002): 153–60. http://dx.doi.org/10.1590/s0100-736x2002000400005.

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A molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2.
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19

Maharana, B. R., Binod Kumar, A. Prasad, T. K. Patbandha, N. R. Sudhakar, J. P. Joseph, and B. R. Patel. "Prevalence and asssessment of risk factors for haemoprotozoan infectionsin cattle and buffaloes of South-West Gujarat, India." Indian Journal of Animal Research, OF (May 19, 2016). http://dx.doi.org/10.18805/ijar.10268.

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A total of 480 suspected blood samples were collected from cattle (n=254) and buffaloes (n=226) during the study period from September 2011 to December 2014 for determination of prevalence of haemoprotozoan parasites from South Western Gujarat (Junagadh). The conventional optical microscopy of Giemsa stained blood smears revealed that 37% of cattle and 38.93% of buffaloes were infected with haemoprotozoan parasites including Anaplasma marginale, Babesia bigemina, Trypanosoma evansi and Theileria annulata. The animals infected with haemoprotozoan diseases showed lower total erythrocyte count (TEC), haemoglobin (Hb), packed cell volume (PCV), mean corpuscular haemoglobin concentration (MCHC) and higher total leukocyte count (TLC) and mean corpuscular volume (MCV). Incidence of haemoprotozoan infection between the breeds differed significantly (P<0.05). Haemoprotozoan infections were recorded significantly higher (P<0.001) in monsoon season followed by summer and being lowest in winter season in cattle. Over all prevalence in female animals were higher than male counterparts. In both the species, over all higher prevalence of babesiosis was recorded in comparison to other haemoprotozoan diseases and the difference being statistically non-significant (p>0.05). The prevalence of B. bigemina in bovines was associated with various risk factors namely age, season and breed (p£0.05). Sex wise females recorded apparently higher infection rates than males. The multivariate logistic regression models showed that the risk of babesiosis was significantly higher in rainy season (OR=5.18, P=0.003) followed by summer (OR=3.9, P=0.019) compared to winter season. Conversely, in buffaloes, the risk of babesiosis was significantly higher in summer season (OR=9.0, P=0.004) followed by rainy (OR=7.43, P=0.008) compared to winter season. The risk for anaplasmosis in buffaloes increased by 3.46 times (OR=3.64, p=0.027) in non descriptive breed compared to well defined breed.
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Wahab, Mohammed, Shahnaz Ali, and Nawzad Abdulrahman. "Identification of Blood Protozoa in See-See Partridges (Ammoperdix Griseogularis) in Garmian Area/Kurdistan Region-Iraq." Al-Anbar Journal of Veterinary Sciences 14, no. 1 (June 30, 2021). http://dx.doi.org/10.37940/ajvs.2021.14.1.5.

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Haemosporidians are intracellular protozoan parasites found inside the blood cells and tissues of avian hosts; they are almost worldwide distributed and occur in a variety of avian species, including see-see partridge birds. Blood samples were collected from 63 see-see partridges during a period extended from 19th November 2018 to 15th March of 2019 in the Garmian area/Iraqi Kurdistan region to detect and investigate the infection rates of haemosporidian parasites by microscopic examination using Giemsa stained smears. The results of the study showed that out of the total samples numbers 63 only 43 were found infected with haemoprotozoan parasites composing the overall infection rate of 68,25%, from 32 birds which were infected with Plasmodium spp. at a rate 50,79%, and 8 birds were infected with Haemoproteus spp. at a rate of 12,69%., the results also revealed that the rates of infection with these protozoan parasites vary substantially among the sampled locations. The present study showed that two haemosporidian genera (Haemoproteus spp. and Plasmodium spp.) were detected in see-see partridges in Kurdistan region-Iraq for the first time, and this type of bird is considered as a new host record for these haemoprotozoa in this area.
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21

Mushi, E. Z., M. G. Binta, R. G. Chabo, R. Ndebele, and R. Panzirah. "Parasites of domestic pigeons (Columba livia domestica) in Sebele, Gaborone, Botswana : short communication." Journal of the South African Veterinary Association 71, no. 4 (July 10, 2000). http://dx.doi.org/10.4102/jsava.v71i4.726.

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The following parasites were found in apparently healthy pigeons kept in Sebele: a haemoprotozoan, Haemoproteus columbae (80 %); endoparasite metazoan nematodes, Ascaridia columbae(30 %) and Dispharynx spiralis(10 %); a cestode, Raillietina sp. (80 %) and coccidian oocysts (40 %); 2 ectoparasites, namely the pigeon fly, Pseudolynchia canariensis (50 %) and the louse, Columbicola columbae (30 %). The pigeons also had high antibody titres, (1:256) to the protozoan parasite Toxoplasma gondii (100 %). The latter infection in these domestic pigeons has public health implications.
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22

Hasan, Al Mahmud, Md Shahadat Hossain, Anita Rani Dey, and Mohammad Zahangir Alam. "Prevalence of malaria parasites in indigenous chickens and ducks in selected districts of Bangladesh." Journal of the Bangladesh Agricultural University 15, no. 2 (December 29, 2017). http://dx.doi.org/10.3329/jbau.v15i2.35072.

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Avian haematozoans are important pathogens of birds causing asymptomatic to fatal infections. The present study was undertaken to investigate the prevalence and associated risk factors of malaria parasite infections in domestic chickens and ducks in three localities namely Mymensingh, Tangail and Netrokona districts in Bangladesh. In total, 474 blood smears (266 chickens and 208 ducks) were screened for haemoprotozoan infection during the period from March, 2016 to February, 2017. Blood samples were collected from wing vein by needle puncture. The parasites were identified from Giemsa stained thin blood smears based on morphological features using standard keys. Malaria protozoa was found in 60 birds (12.7%), of which 35 were chickens (13.2%) and 25 were ducks (12.0%). Two species of blood protozoa were identified namely Leucocytozoon spp. (10.5%) and Plasmodium spp. (2.1%). The prevalence of haemoprotozoa was found high among the adults (13.9%) while it was 11.2% among young birds. Female (15.2%) was 1.46 times more susceptible than male (10.9%).The prevalence rate was highest in Netrokona (18.9%) followed by Tangail (12.2%) and Mymensingh (8.7%). In this study, the prevalence rate was highest in rainy season (18.3%), moderate in summer (10%) and less in winter season (9.3%). Present study revealed that these haemorpotozoa are capable of infecting indigenous domestic chicken and duck. Further studies should focus on characterization of the malaria parasites of indigenous chickens and ducks in Bangladesh.J. Bangladesh Agril. Univ. 15(2): 260-265, December 2017
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23

James Harris, D., Ali Halajian, Joana L. Santos, Lourens H. Swanepoel, Peter John Taylor, and Raquel Xavier. "Diversity of haemoprotozoan parasites infecting the wildlife of South Africa." Folia Parasitologica 65 (October 2, 2018). http://dx.doi.org/10.14411/fp.2018.015.

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24

Patel, M. D., Niranjan Kumar, P. H. Rathod, K. K. Tyagi, and L. M. Sorathiya. "Incidence and Haematological Changes in Haemoprotozoan Infections in Bovines of South Gujarat." INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY 13, no. 02 (November 15, 2017). http://dx.doi.org/10.21887/ijvsbt.v13i02.10054.

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A study was conducted to record the incidence, risk factors and haematological changes during haemoprotozoan infections of bovines over a period from January, 2014 to December, 2016. A total of 193 suspected cases (141 cattle and 52 buffaloes) presented at Teaching Veterinary Clinical Complex and Livestock Research Station, Navsari Agricultural University, Navsari, Gujarat, were examined for the presence of haemoprotozoan parasites by Giemsa staining technique. Total 46 (23.83%) cases (23 each of cattle and buffaloes) were found positive for presence of haemoparasite infection. The effect of source of sample, season and species on incidence of haemoprasitic infections was significant whereas effect of year was non-significant. Higher incidence was observed in rainy (34.92) followed by winter (25.42 %) and summer (12.68%) season. Significantly higher incidence of Anaplasmosis (8.29%) was observed followed by Babesiosis (6.74%), Theileriosis (3.63%) and Trypanosomosis and Ehrlichiosis (2.59% each). All positive cases showed comparatively lower values of haemoglobin (Hb.), packed cell volume (PCV) and total erythrocyte count (TEC) whereas values of total leukocyte count (TLC) and differential leukocyte count (DLC) were within the normal range.
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25

Goodswen, Stephen J., Paul J. Kennedy, and John T. Ellis. "Predicting Protein Therapeutic Candidates for Bovine Babesiosis Using Secondary Structure Properties and Machine Learning." Frontiers in Genetics 12 (July 23, 2021). http://dx.doi.org/10.3389/fgene.2021.716132.

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Bovine babesiosis causes significant annual global economic loss in the beef and dairy cattle industry. It is a disease instigated from infection of red blood cells by haemoprotozoan parasites of the genus Babesia in the phylum Apicomplexa. Principal species are Babesia bovis, Babesia bigemina, and Babesia divergens. There is no subunit vaccine. Potential therapeutic targets against babesiosis include members of the exportome. This study investigates the novel use of protein secondary structure characteristics and machine learning algorithms to predict exportome membership probabilities. The premise of the approach is to detect characteristic differences that can help classify one protein type from another. Structural properties such as a protein’s local conformational classification states, backbone torsion angles ϕ (phi) and ψ (psi), solvent-accessible surface area, contact number, and half-sphere exposure are explored here as potential distinguishing protein characteristics. The presented methods that exploit these structural properties via machine learning are shown to have the capacity to detect exportome from non-exportome Babesia bovis proteins with an 86–92% accuracy (based on 10-fold cross validation and independent testing). These methods are encapsulated in freely available Linux pipelines setup for automated, high-throughput processing. Furthermore, proposed therapeutic candidates for laboratory investigation are provided for B. bovis, B. bigemina, and two other haemoprotozoan species, Babesia canis, and Plasmodium falciparum.
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26

Mushahary, Dipanjali, Kanta Bhattacharjee, Prabhat Sarmah, Dilip Deka, Shantanu Tamuly, and Sanjana Bora. "Molecular Detection and Characterization of Haemoprotozoan and Haemorickettsial Parasites of Cattle in the Indo-Bhutan Border Districts of Assam, India." International Journal of Livestock Research, 2021, 1. http://dx.doi.org/10.5455/ijlr.20201019074125.

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27

Kuleš, Josipa, Ivana Rubić, Blanka Beer Ljubić, Petra Bilić, Renata Barić Rafaj, Mirna Brkljačić, Richard Burchmore, David Eckersall, and Vladimir Mrljak. "Combined Untargeted and Targeted Metabolomics Approaches Reveal Urinary Changes of Amino Acids and Energy Metabolism in Canine Babesiosis With Different Levels of Kidney Function." Frontiers in Microbiology 12 (September 17, 2021). http://dx.doi.org/10.3389/fmicb.2021.715701.

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Canine babesiosis is a tick-borne disease with a worldwide distribution, caused by the haemoprotozoan parasites of the genus Babesia. One of the most prevalent complication is acute kidney injury, and an early diagnosis of altered kidney function remains a challenge for veterinary practice. The aim of this study was to assess the urine metabolic profile from dogs with babesiosis and different degree of kidney function using untargeted and targeted MS-based metabolomics approaches. In this study, 22 dogs naturally infected with Babesia canis and 12 healthy dogs were included. Untargeted metabolomics approach identified 601 features with a differential abundance between the healthy group and groups of dogs with babesiosis and different level of kidney function, with 27 of them identified as a match to known standards; while targeted approach identified 17 metabolites with significantly different concentrations between the groups. A pattern of significantly altered metabolites referring to the inflammatory host response, oxidative stress, and energy metabolism modulation in babesiosis was presented. Our findings have demonstrated that kidney dysfunction accompanying canine babesiosis was associated with changes in amino acid metabolism, energy metabolism, fatty acid metabolism, and biochemical pathways such as urea cycle and ammonia detoxication. These findings will enable the inclusion of urinary markers for the detection and monitoring of renal damage in babesiosis, as well as in other similar diseases.
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28

Masatani, Tatsunori, Shunpei Yoshihara, Atsuko Matsubara, Takafumi Gotoh, Hideyuki Takahashi, Tetsuya Tanaka, Masako Andoh, Yasuyuki Endo, and Tomohide Matsuo. "Dynamics of Theileria orientalis genotype population in cattle in a year-round grazing system." Acta Parasitologica 61, no. 2 (January 1, 2016). http://dx.doi.org/10.1515/ap-2016-0056.

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29

Minnaar, W. N., R. C. Krecek, and J. I. Rajput. "Helminth parasites of dogs from two resource-limited communities in South Africa : research communication." Journal of the South African Veterinary Association 70, no. 2 (July 12, 1999). http://dx.doi.org/10.4102/jsava.v70i2.761.

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Biological samples were collected from dogs in resource-limited communities in the North-West and Gauteng Provinces of South Africa to assess the prevalence of helminth parasitism. These samples included adhesive tape peri-anal skin swabs and fresh faecal samples for helminth examination, and thick and thin blood films (smears) and whole-blood samples in anticoagulant for examination of filarial nematode microfilariae and haemoprotozoa. The eggs of Ancylostoma caninum, Toxocara canis, Toxascaris leonina, Dipylidium caninum and taeniids were identified. None of the blood samples and smears tested positive for microfilariae of Dirofilaria immitis or Dipetalonema spp. or for haemoprotozoa. The adhesive tape swabs were negative for cestode eggs and segments. Most of the helminth parasites identified in this study are zoonotic and consequently are regarded as a public health hazard.
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30

Sivakumar, Thillaiampalam, Shiori Fujita, Bumduuren Tuvshintulga, Hemal Kothalawala, Seekkuge Susil Priyantha Silva, and Naoaki Yokoyama. "Discovery of a new Theileria sp. closely related to Theileria annulata in cattle from Sri Lanka." Scientific Reports 9, no. 1 (November 6, 2019). http://dx.doi.org/10.1038/s41598-019-52512-y.

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Abstract Theileria annulata is a haemoprotozoan parasite that causes a cancer-like illness known as tropical theileriosis in cattle. In the course of analyzing the genetic diversity of T. annulata in Sri Lanka, we observed that merozoite-piroplasm surface antigen (tams1) and surface protein (tasp)-like gene sequences obtained from bovine blood DNA samples, which were PCR-positive for T. annulata, were conserved but shared low identity with T. annulata GenBank sequences. Moreover, the 18S rRNA sequences from the Sri Lankan samples contained ten unique single-nucleotide polymorphisms compared with all known T. annulata sequences. The cytochrome b (cob) gene sequences isolated from the Sri Lankan samples were highly conserved and shared low identity scores with similarly conserved T. annulata sequences from GenBank. Phylogenetic analysis showed that the Sri Lankan tams1-like, tasp-like, 18S rRNA, and cob sequences clustered together and formed sister clades to the common ancestors of all known T. annulata and Theileria lestoquardi sequences. These findings demonstrated that the Sri Lankan cattle were not infected with T. annulata but with a new Theileria sp. (designated as Theileria sp. Yokoyama) closely related to T. annulata.
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