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1

Moller, Abraham G., and Chun Liang. "Determining virus-host interactions and glycerol metabolism profiles in geographically diverse solar salterns with metagenomics." PeerJ 5 (January 10, 2017): e2844. http://dx.doi.org/10.7717/peerj.2844.

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Solar salterns are excellent model ecosystems for studying virus-microbial interactions because of their low microbial diversity, environmental stability, and high viral density. By using the power of CRISPR spacers to link viruses to their prokaryotic hosts, we explored virus-host interactions in geographically diverse salterns. Using taxonomic profiling, we identified hosts such as archaeal Haloquadratum, Halorubrum, and Haloarcula and bacterial Salinibacter, and we found that community composition related to not only salinity but also local environmental dynamics. Characterizing glycerol me
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Akpolat, Can, Ana Beatriz Fernández, Pinar Caglayan, Baris Calli, Meral Birbir, and Antonio Ventosa. "Prokaryotic Communities in the Thalassohaline Tuz Lake, Deep Zone, and Kayacik, Kaldirim and Yavsan Salterns (Turkey) Assessed by 16S rRNA Amplicon Sequencing." Microorganisms 9, no. 7 (2021): 1525. http://dx.doi.org/10.3390/microorganisms9071525.

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Prokaryotic communities and physico-chemical characteristics of 30 brine samples from the thalassohaline Tuz Lake (Salt Lake), Deep Zone, Kayacik, Kaldirim, and Yavsan salterns (Turkey) were analyzed using 16S rRNA amplicon sequencing and standard methods, respectively. Archaea (98.41% of reads) was found to dominate in these habitats in contrast to the domain Bacteria (1.38% of reads). Representatives of the phylum Euryarchaeota were detected as the most predominant, while 59.48% and 1.32% of reads, respectively, were assigned to 18 archaeal genera, 19 bacterial genera, 10 archaeal genera, an
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3

Dyall-Smith, Mike L., Friedhelm Pfeiffer, Kathrin Klee, et al. "Haloquadratum walsbyi : Limited Diversity in a Global Pond." PLoS ONE 6, no. 6 (2011): e20968. http://dx.doi.org/10.1371/journal.pone.0020968.

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4

Elevi Bardavid, Rahel, and Aharon Oren. "Dihydroxyacetone metabolism in Salinibacter ruber and in Haloquadratum walsbyi." Extremophiles 12, no. 1 (2007): 125–31. http://dx.doi.org/10.1007/s00792-007-0114-x.

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5

Burns, David G., Peter H. Janssen, Takashi Itoh, et al. "Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain." International Journal of Systematic and Evolutionary Microbiology 57, no. 2 (2007): 387–92. http://dx.doi.org/10.1099/ijs.0.64690-0.

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Strains C23T and HBSQ001 were isolated from solar salterns and are novel square-shaped, aerobic, extremely halophilic members of the domain Archaea and family Halobacteriaceae. Cells stained Gram-negative and grew optimally in media containing 18 % salts at around neutral pH. Mg2+ is not required. The DNA G+C content of both isolates was 46.9 mol% and DNA–DNA cross-hybridization showed a relatedness of 80 %. Their 16S rRNA gene sequences showed only 2 nucleotide differences (99.9 % identity) and phylogenetic tree reconstructions with other recognized members of the Halobacteriaceae indicated t
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6

Bolhuis, Henk, Ana Belén Martín-Cuadrado, Riccardo Rosselli, Lejla Pašić, and Francisco Rodriguez-Valera. "Transcriptome analysis of Haloquadratum walsbyi: vanity is but the surface." BMC Genomics 18, no. 1 (2017): 510. https://doi.org/10.1186/s12864-017-3892-2.

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<strong>Background: </strong> <i>Haloquadratum walsbyi</i> dominates saturated thalassic lakes worldwide where they can constitute up to 80-90% of the total prokaryotic community. Despite the abundance of the enigmatic square-flattened cells, only 7 isolates are currently known with 2 genomes fully sequenced and annotated due to difficulties to grow them under laboratory conditions. We have performed a transcriptomic analysis of one of these isolates, the Spanish strain HBSQ001 in order to investigate gene transcription under light and dark conditions.<strong>Results: </strong>Despite a potent
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7

Dyall-Smith, Mike, and Friedhelm Pfeiffer. "Global Distribution and Diversity of Haloarchaeal pL6-Family Plasmids." Genes 15, no. 9 (2024): 1123. http://dx.doi.org/10.3390/genes15091123.

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Australian isolates of Haloquadratum walsbyi, a square-shaped haloarchaeon, often harbor small cryptic plasmids of the pL6-family, approximately 6 kb in size, and five examples have been previously described. These plasmids exhibit a highly conserved gene arrangement and encode replicases similar to those of betapleolipoviruses. To assess their global distribution and recover more examples for analysis, fifteen additional plasmids were reconstructed from the metagenomes of seven hypersaline sites across four countries: Argentina, Australia, Puerto Rico, and Spain. Including the five previously
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8

Sublimi Saponetti, Matilde, Fabrizio Bobba, Grazia Salerno, Alessandro Scarfato, Angela Corcelli, and Annamaria Cucolo. "Morphological and Structural Aspects of the Extremely Halophilic Archaeon Haloquadratum walsbyi." PLoS ONE 6, no. 4 (2011): e18653. http://dx.doi.org/10.1371/journal.pone.0018653.

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9

Yalala, Vaishnavi R., Abigeal K. Lynch, and Kenneth V. Mills. "Conditional Alternative Protein Splicing Promoted by Inteins from Haloquadratum walsbyi." Biochemistry 61, no. 4 (2022): 294–302. http://dx.doi.org/10.1021/acs.biochem.1c00788.

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10

Yalala, Vaishnavi, Angel Chavez, Abigeal K. Lynch, and Kenneth V. Mills. "Protein splicing and tryptophan fluorescence of salt‐dependent inteins from Haloquadratum walsbyi." FASEB Journal 34, S1 (2020): 1. http://dx.doi.org/10.1096/fasebj.2020.34.s1.04643.

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11

Lobasso, Simona, Patrizia Lopalco, Rita Vitale, et al. "The Light-Activated Proton Pump Bop I of The Archaeon Haloquadratum walsbyi." Photochemistry and Photobiology 88, no. 3 (2012): 690–700. http://dx.doi.org/10.1111/j.1751-1097.2012.01089.x.

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12

Cassidy, Jennifer, and Francesca Paradisi. "Haloquadratum walsbyi Yields a Versatile, NAD+/NADP+ Dual Affinity, Thermostable, Alcohol Dehydrogenase (HwADH)." Molecular Biotechnology 60, no. 6 (2018): 420–26. http://dx.doi.org/10.1007/s12033-018-0083-6.

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13

Selivanova, Е. А., А. S. Balkin, Yu А. Khlopko, V. Ya Kataev, and А. О. Plotnikov. "The genome of a new <i>Halorubrum distributum</i> strain ICIS4 isolated from the culture of a microalga <i>Dunaliella salina</i>." Microbiology 93, no. 4 (2024): 444–50. https://doi.org/10.31857/s0026365624040075.

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The complete genome sequence of a new strain of the haloarchaeaon Halorubrum distributum ICIS4 was revealed and analyzed. The strain was isolated from a culture of a carotenogenic microalga Dunaliella salina maintained in laboratory conditions for a long period of time. The genome (3.32 Mb) contained 3236 protein-coding genes. Of the 2817 groups of homologous genes, 11 were unique to this strain. In the genome, the genes were revealed, which were responsible for utilization of glycerol and starch and for synthesis of vitamins, pigments, and siderophores. These genes may be involved in formatio
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14

Fu, Hsu-Yuan, Yung-Ning Chang, Ming-Jin Jheng, and Chii-Shen Yang. "Ser262 determines the chloride-dependent colour tuning of a new halorhodopsin from Haloquadratum walsbyi." Bioscience Reports 32, no. 5 (2012): 501–9. http://dx.doi.org/10.1042/bsr20120054.

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Light is an important environmental signal for all organisms on earth because it is essential for physiological signalling and the regulation of most biological systems. Halophiles found in salt-saturated ponds encode various archaeal rhodopsins and thereby harvest various wavelengths of light either for ion transportation or as sensory mediators. HR (halorhodopsin), one of the microbial rhodopsins, senses yellow light and transports chloride or other halides into the cytoplasm to maintain the osmotic balance during cell growth, and it exists almost ubiquitously in all known halobacteria. To d
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15

Oh, Dickson, Kate Porter, Brendan Russ, David Burns, and Mike Dyall-Smith. "Diversity of Haloquadratum and other haloarchaea in three, geographically distant, Australian saltern crystallizer ponds." Extremophiles 14, no. 2 (2009): 161–69. http://dx.doi.org/10.1007/s00792-009-0295-6.

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16

Cuebas-Irizarry, Mara, Ricardo Irizarry-Caro, Carol López-Morales, Keyla Badillo-Rivera, Carlos Rodríguez-Minguela, and Rafael Montalvo-Rodríguez. "Cloning and Molecular Characterization of an Alpha-Glucosidase (MalH) from the Halophilic Archaeon Haloquadratum walsbyi." Life 7, no. 4 (2017): 46. http://dx.doi.org/10.3390/life7040046.

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17

Couto-Rodríguez, Ricardo L., and Rafael Montalvo-Rodríguez. "Temporal Analysis of the Microbial Community from the Crystallizer Ponds in Cabo Rojo, Puerto Rico, Using Metagenomics." Genes 10, no. 6 (2019): 422. http://dx.doi.org/10.3390/genes10060422.

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The Cabo Rojo solar salterns are a hypersaline environment located in a tropical climate, where conditions remain stable throughout the year. These conditions can favor the establishment of steady microbial communities. Little is known about the microbial composition that thrives in hypersaline environments in the tropics. The main goal of this study was to assess the microbial diversity present in the crystallizer ponds of Cabo Rojo, in terms of structure and metabolic processes across time using metagenomic techniques. Three samplings (December 2014, March and July 2016) were carried out, wh
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18

Leoni, Claudia, Caterina Manzari, Hai Tran, et al. "Identification of an Amylomaltase from the Halophilic Archaeon Haloquadratum walsbyi by Functional Metagenomics: Structural and Functional Insights." Life 12, no. 1 (2022): 85. http://dx.doi.org/10.3390/life12010085.

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Amylomaltases are prokaryotic 4-α-glucanotransferases of the GH77 family. Thanks to the ability to modify starch, they constitute a group of enzymes of great interest for biotechnological applications. In this work we report the identification, by means of a functional metagenomics screening of the crystallization waters of the saltern of Margherita di Savoia (Italy), of an amylomaltase gene from the halophilic archaeon Haloquadratum walsbyi, and its expression in Escherichia coli cells. Sequence analysis indicated that the gene has specific insertions yet unknown in homologous genes in prokar
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19

Qiu, Xing-Xing, Mei-Lin Zhao, Dong Han, Wen-Jiao Zhang, Mike L. Dyall-Smith, and Heng-Lin Cui. "Taxonomic study of the genera Halogeometricum and Halosarcina : transfer of Halosarcina limi and Halosarcina pallida to the genus Halogeometricum as Halogeometricum limi comb. nov. and Halogeometricum pallidum comb. nov., respectively." International Journal of Systematic and Evolutionary Microbiology 63, Pt_10 (2013): 3915–19. http://dx.doi.org/10.1099/ijs.0.055038-0.

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Members of the haloarchaeal genera Halosarcina and Halogeometricum (family Halobacteriaceae ) are closely related to each other and show 96.6–98 % 16S rRNA gene sequence similarity. This is higher than the accepted threshold value (95 %) to separate two genera, and a taxonomic study using a polyphasic approach of all four members of the two genera was conducted to clarify their relationships. Polar lipid profiles indicated that Halogeometricum rufum RO1-4T, Halosarcina pallida BZ256T and Halosarcina limi RO1-6T are related more to each other than to Halogeometricum borinquense CGMCC 1.6168T. P
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20

Tully, Benjamin J., Joanne B. Emerson, Karen Andrade, et al. "De NovoSequences ofHaloquadratum walsbyifrom Lake Tyrrell, Australia, Reveal a Variable Genomic Landscape." Archaea 2015 (2015): 1–12. http://dx.doi.org/10.1155/2015/875784.

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Hypersaline systems near salt saturation levels represent an extreme environment, in which organisms grow and survive near the limits of life. One of the abundant members of the microbial communities in hypersaline systems is the square archaeon,Haloquadratum walsbyi. Utilizing a short-read metagenome from Lake Tyrrell, a hypersaline ecosystem in Victoria, Australia, we performed a comparative genomic analysis ofH. walsbyito better understand the extent of variation between strains/subspecies. Results revealed that previously isolated strains/subspecies do not fully describe the complete reper
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Chen, Yong, Fengfeng Zhou, Guojun Li, and Ying Xu. "MUST: A system for identification of miniature inverted-repeat transposable elements and applications to Anabaena variabilis and Haloquadratum walsbyi." Gene 436, no. 1-2 (2009): 1–7. http://dx.doi.org/10.1016/j.gene.2009.01.019.

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22

Gómez-Villegas, Patricia, Javier Vigara, and Rosa León. "Characterization of the Microbial Population Inhabiting a Solar Saltern Pond of the Odiel Marshlands (SW Spain)." Marine Drugs 16, no. 9 (2018): 332. http://dx.doi.org/10.3390/md16090332.

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The solar salterns located in the Odiel marshlands, in southwest Spain, are an excellent example of a hypersaline environment inhabited by microbial populations specialized in thriving under conditions of high salinity, which remains poorly explored. Traditional culture-dependent taxonomic studies have usually under-estimated the biodiversity in saline environments due to the difficulties that many of these species have to grow at laboratory conditions. Here we compare two molecular methods to profile the microbial population present in the Odiel saltern hypersaline water ponds (33% salinity).
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23

Peters, Danielle L., Bassel Akache, Wangxue Chen, and Michael J. McCluskie. "In Silico Prophage Analysis of Halobacterium salinarum ATCC 33170." Applied Microbiology 4, no. 2 (2024): 607–19. http://dx.doi.org/10.3390/applmicrobiol4020042.

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The extremophile Halobacterium salinarum is an aerobic archaeon that has adapted to thrive in high-salt environments such as salted fish, hypersaline lakes, and salterns. Halophiles have garnered significant interest due to their unique interactions with bacteriophages known as haloarchaeophages. Studies have identified and characterized prophages in halophilic archaea, such as Haloferax volcanii, Haloquadratum walsbyi, and Haloarcula marismortui. Still, an investigation has yet to be conducted into the presence of prophage elements on Halobacterium salinarum ATCC 33170. This is of particular
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24

Creamean, Jessie M., Julio E. Ceniceros, Lilyanna Newman, et al. "Evaluating the potential for Haloarchaea to serve as ice nucleating particles." Biogeosciences 18, no. 12 (2021): 3751–62. http://dx.doi.org/10.5194/bg-18-3751-2021.

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Abstract. Aerosols play a crucial role in cloud formation. Biologically derived materials from bacteria, fungi, pollen, lichen, viruses, algae, and diatoms can serve as ice nucleating particles (INPs), some of which initiate glaciation in clouds at relatively warm freezing temperatures. However, determining the magnitude of the interactions between clouds and biologically derived INPs remains a significant challenge due to the diversity and complexity of bioaerosols and limited observations of such aerosols facilitating cloud ice formation. Additionally, microorganisms from the domain Archaea
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Leoni, Claudia, Mariateresa Volpicella, Bruno Fosso, et al. "A Differential Metabarcoding Approach to Describe Taxonomy Profiles of Bacteria and Archaea in the Saltern of Margherita di Savoia (Italy)." Microorganisms 8, no. 6 (2020): 936. http://dx.doi.org/10.3390/microorganisms8060936.

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Microorganisms inhabiting saline environments are an interesting ecological model for the study of the adaptation of organisms to extreme living conditions and constitute a precious resource of enzymes and bioproducts for biotechnological applications. We analyzed the microbial communities in nine ponds with increasing salt concentrations (salinity range 4.9–36.0%) of the Saltern of Margherita di Savoia (Italy), the largest thalassohaline saltern in Europe. A deep-metabarcoding NGS procedure addressing separately the V5-V6 and V3-V4 hypervariable regions of the 16S rRNA gene of Bacteria and Ar
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Shaffer, Justin P., Louis-Félix Nothias, Luke R. Thompson, et al. "Standardized multi-omics of Earth’s microbiomes reveals microbial and metabolite diversity." Nature Microbiology 7, no. 12 (2022): 2128–50. http://dx.doi.org/10.1038/s41564-022-01266-x.

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AbstractDespite advances in sequencing, lack of standardization makes comparisons across studies challenging and hampers insights into the structure and function of microbial communities across multiple habitats on a planetary scale. Here we present a multi-omics analysis of a diverse set of 880 microbial community samples collected for the Earth Microbiome Project. We include amplicon (16S, 18S, ITS) and shotgun metagenomic sequence data, and untargeted metabolomics data (liquid chromatography-tandem mass spectrometry and gas chromatography mass spectrometry). We used standardized protocols a
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Elevi Bardavid, Rahel, and Aharon Oren. "Sensitivity of Haloquadratum and Salinibacter to antibiotics and other inhibitors: implications for the assessment of the contribution of Archaea and Bacteria to heterotrophic activities in hypersaline environments." FEMS Microbiology Ecology 63, no. 3 (2008): 309–15. http://dx.doi.org/10.1111/j.1574-6941.2007.00433.x.

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Cui, Heng-Lin, Xin Yang, Xia Gao, and Xue-Wei Xu. "Halobellus clavatus gen. nov., sp. nov. and Halorientalis regularis gen. nov., sp. nov., two new members of the family Halobacteriaceae." International Journal of Systematic and Evolutionary Microbiology 61, no. 11 (2011): 2682–89. http://dx.doi.org/10.1099/ijs.0.025841-0.

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Four halophilic archaeal strains, designated TNN18T, TBN12, TNN28T and TBN19, were isolated from brines sampled from two artificial marine solar salterns in eastern China. Strains TNN18T and TNN28T were isolated from the Tainan marine solar saltern, whereas strains TBN12 and TBN19 were from the Taibei marine solar saltern. Colonies of the four strains were red-pigmented and their cells were pleomorphic, motile, Gram-reaction-negative rods. Strains TNN18T and TBN12 were able to grow at 25–50 °C (optimum 37 °C), in 10–30 % (w/v) NaCl (optimum 15 %), with 0–1.0 M MgCl2 (optimum 0.05 M) and at pH
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Dyall-Smith, Mike, and Friedhelm Pfeiffer. "The PL6-Family Plasmids of Haloquadratum Are Virus-Related." Frontiers in Microbiology 9 (May 23, 2018). http://dx.doi.org/10.3389/fmicb.2018.01070.

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Lynch, Abigeal Kathleen, Shirley Amunya, Alvin Gomez, Julie Reitter, and Kenneth Mills. "Differential protein splicing of salt‐dependent inteins from Haloquadratum walsbyi." FASEB Journal 32, S1 (2018). http://dx.doi.org/10.1096/fasebj.2018.32.1_supplement.655.18.

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31

Conroy, Owen R., Alvin V. Gomez, and Kenneth V. Mills. "The Variable Salt Dependence of Mini‐Inteins from Haloquadratum walsbyi." FASEB Journal 32, S1 (2018). http://dx.doi.org/10.1096/fasebj.2018.32.1_supplement.655.22.

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32

Lynch, Abigeal, Shirley Amunya, Alvin Gomez, Julie N. Reitter, and Kenneth Mills. "Differential protein splicing of salt‐dependent inteins from Haloquadratum walsbyi." FASEB Journal 33, S1 (2019). http://dx.doi.org/10.1096/fasebj.2019.33.1_supplement.633.18.

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33

Bolhuis, Henk, Ana Belén Martín-Cuadrado, Riccardo Rosselli, Lejla Pašić, and Francisco Rodriguez-Valera. "Transcriptome analysis of Haloquadratum walsbyi: vanity is but the surface." BMC Genomics 18, no. 1 (2017). http://dx.doi.org/10.1186/s12864-017-3892-2.

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Lynch, Abigeal Kathleen, Shirley Amunya, Julie Reitter, and Kenneth Mills. "Differential Protein Splicing of Salt Dependent Inteins from Haloquadratum Walsybi." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.606.13.

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Protein splicing is a self‐catalyzed, post‐translational process in which an intervening polypeptide (intein) catalyzes its own removal from the flanking polypeptides (exteins), as well as the ligation of the exteins. We are interested in the splicing of two inteins interrupting the cdc21 protein from Haloquadratum walsbyi (Hwa). Hwa is a halophilic archaeon. Because of this, we hypothesized that protein splicing would be salt dependent. The first intein, Cdc21a, does not splice on overexpression in E. coli, but can be induced to splice in vitro on incubation with 2.5 M sodium chloride at 28°C
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Ko, Ling-Ning, Guo Zhen Lim, Jui-Chien Chen, Ta Ko, Guan-Yi Li, and Chii-Shen Yang. "Rhodopsin from Haloquadratum walsbyi is a light-driven magnesium transporter." Nature Communications 16, no. 1 (2025). https://doi.org/10.1038/s41467-025-59795-y.

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Rosselli, Riccardo, Mario López-Pérez, Ana-Belen Martin-Cuadrado, Francisco Rodriguez-Valera, and Henk Bolhuis. "Differences in gene expression patterns between cultured and natural Haloquadratum walsbyi ecotypes." Frontiers in Microbiology 13 (November 10, 2022). http://dx.doi.org/10.3389/fmicb.2022.1044446.

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Solar crystallizer ponds are characterized by high population density with a relatively simple community structure in terms of species composition. The microbial community in the solar saltern of Santa Pola (Alicante, Spain), is largely dominated by the hyperhalophilic square archaeon Haloquadratum walsbyi. Here we studied metatranscriptomes retrieved from a crystallizer pond during the winter of 2012 and summer of 2014 and compared Hqr. walsbyi’s transcription patterns with that of the cultured strain Hqr. walsbyi HBSQ001. Significant differences were found between natural and the cultured gr
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Martin-Cuadrado, Ana-Belen, Lejla Pašić, and Francisco Rodriguez-Valera. "Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi." BMC Genomics 16, no. 1 (2015). http://dx.doi.org/10.1186/s12864-015-1794-8.

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Zenke, Ralf, Susanne von Gronau, Henk Bolhuis, Manuela Gruska, Friedhelm Pfeiffer, and Dieter Oesterhelt. "Fluorescence microscopy visualization of halomucin, a secreted 927 kDa protein surrounding Haloquadratum walsbyi cells." Frontiers in Microbiology 6 (March 30, 2015). http://dx.doi.org/10.3389/fmicb.2015.00249.

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D'Angelo, Anthony C., Vaishnavi R. Yalala, and Kenneth V. Mills. "A Directed Evolution Experiment to Improve Protein Splicing of Two Inteins From Haloquadratum walsbyi." FASEB Journal 36, S1 (2022). http://dx.doi.org/10.1096/fasebj.2022.36.s1.r4196.

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Bolhuis, Henk, Peter Palm, Andy Wende, et al. "The genome of the square archaeon Haloquadratum walsbyi : life at the limits of water activity." BMC Genomics 7, no. 1 (2006). http://dx.doi.org/10.1186/1471-2164-7-169.

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Reidy, Deirdre Ann. "Salt‐Dependent Protein Splicing of the Intein in the Haloquadratum walsbyi DNA Polymerase." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.606.12.

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Protein splicing is a post‐translational process by which an intervening polypeptide, or intein, can excise itself and ligate the flanking polypeptides, or exteins. Two extreme halophilic archaea, Halobacterium salinarum (Hsa) and Haloquadratum walsbyi (Hwa), have proteins with inteins. Previous work in the lab demonstrated that splicing of the Hsa DNA Polymerase II (Polll) intein is salt‐dependent. We attempted to compare the homologous PolII‐a intein in Hwa. However, the protein was insoluble when expressed in two different strains of E. coli and with addition of salt. Currently, we are atte
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Kheiri, Roohollah, Maliheh Mehrshad, Ahmad Ali Pourbabaee, Antonio Ventosa, and Mohammad Ali Amoozegar. "Hypersaline Lake Urmia: a potential hotspot for microbial genomic variation." Scientific Reports 13, no. 1 (2023). http://dx.doi.org/10.1038/s41598-023-27429-2.

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AbstractLake Urmia located in Iran is a hypersaline environment with a salinity of about 27% (w/v). Metagenomic analyses of water samples collected from six locations in the lake exhibited a microbial community dominated by representatives of the family Haloferacaceae (69.8%), mainly those affiliated to only two genera, Haloquadratum (59.3%) and Halonotius (9.1%). Similar to other hypersaline lakes, the bacterial community was dominated by Salinibacter ruber (23.3%). Genomic variation analysis by inspecting single nucleotide variations (SNVs) and insertions/deletions (INDELs) exhibited a high
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43

Oren, Aharon. "Novel insights into the diversity of halophilic microorganisms and their functioning in hypersaline ecosystems." npj Biodiversity 3, no. 1 (2024). http://dx.doi.org/10.1038/s44185-024-00050-w.

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AbstractOur understanding of the microbial diversity inhabiting hypersaline environments, here defined as containing &gt;100–150 g/L salts, has greatly increased in the past five years. Halophiles are found in each of the three domains of life. Many novel types have been cultivated, and metagenomics and other cultivation-independent approaches have revealed the existence of many previously unrecognized lineages. Syntrophic interactions between different phylogenetic lineages have been discovered, such as the symbiosis between members of the archaeal class Halobacteria and the ‘Candidatus Nanoh
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Zhang, Wanlin, Abigeal K. Lynch, Alvin O. Gomez, Julie N. Reitter, and Kenneth V. Mills. "A structural investigation and splicing study of inteins from Halobacteria." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.606.15.

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Protein splicing is a post‐translational modification by which a segment of protein, known as an intein, self‐catalyzes its excision from the flanking protein while simultaneously ligating the two flanking exteins. Inteins can be found in many types of archaea, such as halophiles, which specifically thrive in environments with high salt concentrations. The lab previously reported that the Halobacterium salinarum (Hsa) DNA polymerase II (Pol II) intein promotes conditional protein splicing at high concentrations of sodium chloride. To study the structure of the intein, we attempted to find cond
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45

Cusenza, Benvenuta Sonia, Giuseppe Scelfo, Gabriella Licata, et al. "First Insights Into the Biological and Physical–Chemical Diversity of Various Salt Ponds of Trapani, Sicily." Environmental Microbiology Reports 17, no. 2 (2025). https://doi.org/10.1111/1758-2229.70075.

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ABSTRACTThe salt ponds of Trapani, Sicily, represent an extreme and under‐explored ecosystem characterised by varying salinity gradients and environmental conditions. These ponds, integral to traditional salt extraction, include cold, driving, hot and crystallizer ponds, each hosting diverse microbial communities. This study aimed to explore the biological and physical–chemical diversity of 11 ponds during the salt production season in Trapani. We conducted comprehensive physical–chemical characterizations, including measurements of pH, conductivity, viscosity, density, organic carbon and ion
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46

Legault, Boris A., Arantxa Lopez-Lopez, Jose Carlos Alba-Casado, et al. "Environmental genomics of "Haloquadratum walsbyi" in a saltern crystallizer indicates a large pool of accessory genes in an otherwise coherent species." BMC Genomics 7, no. 1 (2006). http://dx.doi.org/10.1186/1471-2164-7-171.

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47

Mirete, Salvador, María Lamprecht-Grandío, Carolina González de Figueras, and José Eduardo González-Pastor. "Domain-specific osmoadaptation revealed by metatranscriptomic analysis in hypersaline environments." Scientific Reports 15, no. 1 (2025). https://doi.org/10.1038/s41598-025-04148-4.

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Abstract High-throughput RNA-seq enables the analysis of gene expression in complex, culture-independent microbial communities. In this study, we used this approach to explore the microbial adaptation to salinity changes in hypersaline environments using samples collected from the Santa Pola ponds (Alicante, Spain). Two metatranscriptomic experiments were conducted: (i) salt concentration from 20 to 30%, mimicking summer evaporation, and (ii) dilution from 30 to 25%, simulating rainfall. As a result, these two experiments revealed significant differences in the gene expression of several metab
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48

Gomez, Alvin, and Kenneth V. Mills. "Conditional protein splicing of inteins from extremophiles." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.606.19.

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Protein splicing is a post‐translational process that results in the removal of an intervening polypeptide, the intein, and the ligation of the flanking C‐ and N‐terminal polypeptides, the exteins, to form a mature protein. Although protein splicing occurs without the need for any cofactor, chaperone or energy source, the proper conformation of the active site may be influenced by environmental cues. Undergraduates in our lab have carried out experiments to study the structure and mechanism of inteins from halophiles and deep‐sea thermophiles. We have shown that an intein from Halobacterium sa
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Bustos-Caparros, Esteban, Tomeu Viver, Juan F. Gago, et al. "Ecological success of extreme halophiles subjected to recurrent osmotic disturbances is primarily driven by congeneric species replacement." ISME Journal, October 23, 2024. http://dx.doi.org/10.1093/ismejo/wrae215.

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Abstract To understand how extreme halophiles respond to recurrent disturbances, we challenged the communities thriving in salt-saturated (~36% salts) ~230 L brine mesocosms to repeated dilutions down to 13% (D13 mesocosm) or 20% (D20 mesocosm) salts each time mesocosms reached salt saturation due to evaporation (for 10 and 17 cycles, respectively) over 813 days. Depending on the magnitude of dilution, the most prevalent species, Haloquadratum walsbyi and Salinibacter ruber, either increased in dominance by replacing less competitive populations (for D20, moderate stress conditions), or severe
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50

García-Roldán, Alicia, la Haba Rafael de, Cristina Sánchez-Porro, and Anotnio Ventosa. "'Altruistic' cooperation among the prokaryotic community of Atlantic salterns assessed by metagenomics." Microbiological Research, October 5, 2024. https://doi.org/10.1016/j.micres.2024.127869.

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Hypersaline environments are extreme habitats with a limited prokaryotic diversity, mainly restricted to halophilicor halotolerant archaeal and bacterial taxa adapted to highly saline conditions. This study attempts toanalyze the taxonomic and functional diversity of the prokaryotes that inhabit a solar saltern located at theAtlantic Coast, in Isla Cristina (Huelva, Southwest Spain), and the influence of salinity on the diversity andmetabolic potential of these prokaryotic communities, as well as the interactions and cooperation among theindividuals within that community. Brine samples were ob
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