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1

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, and Snehasis Jana. "Comparative Physicochemical Evaluation of Biofield Treated Phosphate Buffer Saline and Hanks Balanced Salt Medium." American Journal of BioScience 3, no. 6 (2015): 267–77. https://doi.org/10.11648/j.ajbio.20150306.20.

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Phosphate buffer saline (PBS) has numerous biological and pharmaceutical applications. Hank buffer salt (HBS) has been used as a medium for tissue culture applications. This research study was aimed to investigate the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of the PBS and HBS. The study was executed in two group’s i.e. control and treated. The control group was kept aside as control and treated group had received the biofield energy treatment. The control and treated samples were further characterized by X-ray diffraction (XRD), differenti
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2

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Mishra, and Snehasis Jana. "Comparative Physicochemical Evaluation of Biofield Treated Phosphate Buffer Saline and Hanks Balanced Salt Medium." American Journal of BioScience 3, no. 6 (2015): 267–77. https://doi.org/10.5281/zenodo.192643.

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Phosphate buffer saline (PBS) has numerous biological and pharmaceutical applications. Hank buffer salt (HBS) has been used as a medium for tissue culture applications. This research study was aimed to investigate the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of the PBS and HBS. The study was executed in two group’s i.e. control and treated. The control group was kept aside as control and treated group had received the biofield energy treatment. The control and treated samples were further characterized by X-ray diffraction (XRD),
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3

Chinnapen, Himani, Laurent Boissel, Thomas Bickett, et al. "Abstract 890: NK-92® (aNK™) whole cell lysate exerts potent cytotoxic and anti-proliferative activity on tumor cells." Cancer Research 85, no. 8_Supplement_1 (2025): 890. https://doi.org/10.1158/1538-7445.am2025-890.

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Lysates from human cells represent biofluids that are used in the biotechnology field for a number of applications, such as biomarker identification and antibody detection. Lysate from human blood platelets is widely used in the clinical setting to control bleeding. We hypothesized that whole cell lysate prepared from the natural killer cell line NK-92® or ‘activated’ NK (aNK™) has the potential as an immunotherapy modality for local treatment of cancer lesions, as it would be expected to contain an abundance of cytotoxic granule-associated serine proteases (granzymes), the pore-forming cytoly
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4

Kumar Trivedi, Mahendra. "Comparative Physicochemical Evaluation of Biofield Treated Phosphate Buffer Saline and Hanks Balanced Salt Medium." American Journal of BioScience 3, no. 6 (2015): 267. http://dx.doi.org/10.11648/j.ajbio.20150306.20.

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5

Tanaka, J., T. Watanabe, N. Nakamura, and K. Sobue. "Morphological and biochemical analyses of contractile proteins (actin, myosin, caldesmon and tropomyosin) in normal and transformed cells." Journal of Cell Science 104, no. 2 (1993): 595–606. http://dx.doi.org/10.1242/jcs.104.2.595.

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The expression and intracellular distribution of four contractile proteins (actin, myosin, caldesmon and tropomyosin) in normal fibroblasts and their transformed counterparts by Rous or avian sarcoma virus were compared. By analyzing the isoformal expression of actin, caldesmon and tropomyosin using two-dimensional gel electrophoresis, only tropomyosin showed significant alteration in its isoformal expression accompanied by transformation. Morphological study revealed that in normal cells, myosin, caldesmon and tropomyosin were distributed periodically along stress fibers, but were excluded fr
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6

Sato, Atsushi, Hachidai Aizawa, Tetsuhiro Tsujino, et al. "Fluorescent Cytochemical Detection of Polyphosphates Associated with Human Platelets." International Journal of Molecular Sciences 22, no. 3 (2021): 1040. http://dx.doi.org/10.3390/ijms22031040.

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Polyphosphate (polyP) is released from activated platelets and activates the intrinsic coagulation pathway. However, polyP may also be involved in various pathophysiological functions related to platelets. To clarify these functions, we established a cytochemical method to reproducibly visualize polyP in platelets. Platelets obtained from healthy non-smoking donors were suspended in phosphate-buffered saline and quickly immobilized on glass slides using a Cytospin. After fixation and membrane permeabilization, platelets were treated with 4′,6- diamidino-2-phenylindole (DAPI) and examined using
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7

Strauch, Bettina, Martin Zimmer, and Rik Tjallingii. "Lab scale salt caverns – first results on construction and investigation techniques." Advances in Geosciences 49 (October 2, 2019): 149–54. http://dx.doi.org/10.5194/adgeo-49-149-2019.

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Abstract. Salt caverns from solution mining in bedded or domal saline structures are of increasing importance as temporary subsurface storage space to buffer fluctuating renewables and secure a stable energy supply. To assure the integrity of caverns during operation and long term abandonment, knowledge of geochemical rock–water interactions in the transition zone between cavity and salt rock is necessary. Due to the inaccessibility of cavern walls, a set of lab-based experiments were performed in hand-sized specimens by creating cm-sized cavities using fresh water injection and brine removal.
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Thowsen, Irene Matre, Tore Reikvam, Trude Skogstrand, et al. "Genetic Engineering of Lymphangiogenesis in Skin Does Not Affect Blood Pressure in Mouse Models of Salt-Sensitive Hypertension." Hypertension 79, no. 11 (2022): 2451–62. http://dx.doi.org/10.1161/hypertensionaha.122.19777.

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Background: Recent studies have indicated that sodium storage is influenced by macrophages that secrete VEGF-C (vascular endothelial growth factor) during salt stress thus stimulating lymphangiogenesis, thereby acting as a buffer against increased blood pressure (BP). We aimed to explore the role of dermal lymphatics in BP and sodium homeostasis. Our hypothesis was that mice with reduced dermal lymphatic vessels were more prone to develop salt-sensitive hypertension, and that mice with hyperplastic vessels were protected. Methods: Mice with either hypoplastic (Chy), absent (K14-VEGFR3 [vascula
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9

Moriguchi, Keiichi, and Kei-Ichi Hirai. "Phagocytosis and hydrogen peroxide production of Eosinophils in the rats spontaneously infected with Mycoplasma pulmonis." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 314–15. http://dx.doi.org/10.1017/s0424820100159126.

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The ability of hydrogen peroxide (H2O2) production was cytochemically compared in eosinophils (EP) between specific pathogen free (SPF) and spontaneously Mycoplasma pulmonis-infected male rats (Wistar strain).Peritoneal cells including EP (PEP) were harvested with a cold Hanks' balanced salt solution containing 0.1% glucose (HBSSG). Simultaneously, blood granulocytes with eosinophils (BEP) were isolated from the same animals by a Ficoll-Hypaque method. Cells were incubated with latex particles in HBSSG for 60-90 min. Thereafter, cells were washed and transferred into a cerium (Ce) medium consi
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10

Smitley, D. R., T. W. Davis, K. A. Kearns, and C. A. Stevens. "European Chafer Larvae Control, Belmont, Mich., 1990." Insecticide and Acaricide Tests 16, no. 1 (1991): 263. http://dx.doi.org/10.1093/iat/16.1.263.

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Abstract Curative treatments: A grid of 3 × 3 ft plots separated by 2 ft wide buffer strips was established at Blythefield Country Club. Six replications of 11 treatments were applied on 20 Aug between 9:00 and 11:00 AM. Temperature at application was approximately 70°F with a slight rainfall (0.21 inches) about 9:30 AM followed by sunny weather conditions. The plots were established on an irrigated rough with sandy soil. Irrigation was applied approximately every other day depending on rainfall. Insect parasitic nematodes were applied while the grass was wet with dew and then hand irrigated w
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11

M. Roshdy, Amira, Nesreen H. Abou - Baker, and Omaima A. Sharaf. "Production Physiology and Biochemical properties of Crude Thermophile Protease by Mild - Halophilic Bacillus thuringiensis aizawai HD 865." Journal of Modern Agriculture and Biotechnology 3 (October 25, 2024): 8. http://dx.doi.org/10.53964/jmab.2024008.

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Objective: The current study aims to enrich the research library of Bacillus thuringensis, the most famous bacteria as a biopesticide, with a product that may enhance its ability as a biopesticide as well as its many other applications, namely the heat-tolerant alkaline protease. Methods: The strain Bacillus thuringiensis aizawai HD 865, obtained from HD Culture Collection was grown on a liquid growth medium of nutrient broth and yeast extract, and colonies were counted on agar plates. The zygote is then transferred to a skim milk environment to estimate the enzymatic activity under the differ
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12

Smitley, D. R., T. W. Davis, K. A. Kearns, and C. M. Papp. "European Chafer Larvae Control, Michigan, 1991." Insecticide and Acaricide Tests 17, no. 1 (1992): 319. http://dx.doi.org/10.1093/iat/17.1.319.

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Abstract A grid of 3 ft × 3 ft plots separated by 1 ft wide buffer strips was established at Blythefield County Club in Belmont, MI. The plots were placed on irrigated rough with sandy soil. Each insecticide treatment was replicated six times. Granular insecticides were applied with modified “salt” shakers. Insecticides were mixed with 1500 ml of water and applied at a rate of 137 ml/9 ft2 (175 gal/ acre) with a single nozzle, hand-held wand C02 sprayer from R&D Sprayers. The application was made at 50 psi through an 8003 flat fan nozzle. Several of the NTN 33893 treatments were applied in
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13

Gavin, Muriel, Carol L. Wells, and Stanley L. Erlandsen. "High-resolution SEM studies of the attachment of Giardia trophozoites to the microvillous border of cultured intestinal epithelial cells." Proceedings, annual meeting, Electron Microscopy Society of America 51 (August 1, 1993): 370–71. http://dx.doi.org/10.1017/s0424820100147697.

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Trophozoites or the free-swimming form of the intestinal protozoan, Giardia, resides within the host small intestine where they attach to the microvillous border by means of a grasping organelle, the ventral adhesive disc. Several mechanisms have been proposed to explain trophozoite attachment including contractile activity, microtubular coiling-uncoiling, hydryodynamic activity of the ventral flagella, and activated lectin binding.We have developed a model for investigating the mechanism of attachment of Giardia trophozoites to the microvillous border of cultured intestinal epithelial cells.
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14

Marunaka, Yoshinori. "The Proposal of Molecular Mechanisms of Weak Organic Acids Intake-Induced Improvement of Insulin Resistance in Diabetes Mellitus via Elevation of Interstitial Fluid pH." International Journal of Molecular Sciences 19, no. 10 (2018): 3244. http://dx.doi.org/10.3390/ijms19103244.

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Blood contains powerful pH-buffering molecules such as hemoglobin (Hb) and albumin, while interstitial fluids have little pH-buffering molecules. Thus, even under metabolic disorder conditions except severe cases, arterial blood pH is kept constant within the normal range (7.35~7.45), but the interstitial fluid pH under metabolic disorder conditions becomes lower than the normal level. Insulin resistance is one of the most important key factors in pathogenesis of diabetes mellitus, nevertheless the molecular mechanism of insulin resistance occurrence is still unclear. Our studies indicate that
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15

Jenkins, Jill A., Bruce E. Eilts, Amy M. Guitreau, Chester R. Figiel, Rassa O. Draugelis-Dale, and Terrence R. Tiersch. "Sperm quality assessments for endangered razorback suckers Xyrauchen texanus." REPRODUCTION 141, no. 1 (2011): 55–65. http://dx.doi.org/10.1530/rep-10-0153.

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Flow cytometry (FCM) and computer-assisted sperm motion analysis (CASA) methods were developed and validated for use with endangered razorback suckersXyrauchen texanuscollected (n=64) during the 2006 spawning season. Sperm motility could be activated within osmolality ranges noted during milt collections (here 167–343 mOsm/kg). We hypothesized that sperm quality of milt collected into isoosmotic (302 mOsm/kg) or hyperosmotic (500 mOsm/kg) Hanks' balanced salt solution would not differ. Pre-freeze viabilities were similar between osmolalities (79%±6 (s.e.m.) and 76%±7); however, post-thaw value
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16

Tatzber, Franz, Willibald Wonisch, Gyula Balka, et al. "Coating with Hypertonic Saline Improves Virus Protection of Filtering Facepiece Manyfold—Benefit of Salt Impregnation in Times of Pandemic." International Journal of Environmental Research and Public Health 18, no. 14 (2021): 7406. http://dx.doi.org/10.3390/ijerph18147406.

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Recently, as is evident with the COVID-19 pandemic, virus-containing aerosols can rapidly spread worldwide. As a consequence, filtering facepieces (FFP) are essential tools to protect against airborne viral particles. Incorrect donning and doffing of masks and a lack of hand-hygiene cause contagion by the wearers’ own hands. This study aimed to prove that hypertonic saline effectively reduces the infectious viral load on treated masks. Therefore, a hypertonic salt solution´s protective effect on surgical masks was investigated, specifically analyzing the infectivity of aerosolized Alphacoronav
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17

Rogstam, Annika, Sara Linse, Anders Lindqvist, Peter James, Ludwig Wagner, and Tord Berggård. "Binding of calcium ions and SNAP-25 to the hexa EF-hand protein secretagogin." Biochemical Journal 401, no. 1 (2006): 353–63. http://dx.doi.org/10.1042/bj20060918.

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Secretagogin is a hexa EF-hand protein, which has been identified as a novel potential tumour marker. In the present study, we show that secretagogin binds four Ca2+ ions (log K1=7.1±0.4, log K2=4.7±0.6, log K3=3.6±0.7 and log K4=4.6±0.6 in physiological salt buffers) with a [Ca2+]0.5 of approx. 25 μM. The tertiary structure of secretagogin changes significantly upon Ca2+ binding, but not upon Mg2+ binding, and the amount of exposed hydrophobic surface in secretagogin increases upon Ca2+ binding, but not upon Mg2+ binding. These properties suggest that secretagogin belongs to the ‘sensor’ fami
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18

Wang, Cheng, Xiao Liu, Di Mei, et al. "Local pH and oxygen concentration at the interface of Zn alloys in Tris-HCl or HEPES buffered Hanks’ balanced salt solution." Corrosion Science 197 (April 2022): 110061. http://dx.doi.org/10.1016/j.corsci.2021.110061.

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19

Smitley, D. R., and T. W. Davis. "European Chafer Larvae Control, Michigan, 1992." Insecticide and Acaricide Tests 18, no. 1 (1993): 343–44. http://dx.doi.org/10.1093/iat/18.1.343a.

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Abstract A grid of 3 ft X 3ft plots separated by 1 ft wide buffer strips was used for this test, at a golf course in Belmont, MI. The plots were located on irrigated rough with sandy soil. Each treatment was replicated six times. Granular insecticides were applied with modified “salt” shakers. Insecticides were mixed with 1500 ml of water and applied at a rate of 137 ml/9 ft2 (175 gal/acre) with a single nozzle, hand-held wand C02 sprayer from R&D Sprayers. Insecticide applications were made at 50 psi through an 8003 flat fan nozzle. The nematode treatments were applied at 10 psi through a
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20

Alatas, Fikri, Morisa Aprilliana, and Dolih Gozali. "THE PREPARATION AND SOLUBILITY OF LORATADINE-FUMARIC ACID BINARY MIXTURE." Asian Journal of Pharmaceutical and Clinical Research 10, no. 1 (2016): 331. http://dx.doi.org/10.22159/ajpcr.2017.v10i1.15400.

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ABSTRACTObjective: Loratadine (LOR) is a biopharmaceutics classification system Class II drug that has low solubility and high permeability, so its absorptionrate is determined by the dissolution rate. The aim of this study was to prepare an LOR-fumaric acid (LOR-FUM) binary mixture and to investigate itsinfluence on the solubility and dissolution rate of LOR.Methods: LOR-FUM binary mixture was prepared by the wet grinding method in an equimolar ratio by the addition of two drops of methanol.Characterization of LOR-FUM binary mixture was conducted by the construction of the phase solubility, p
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21

ШОЛИДОДОВ, М. Р., А. Р. САЙДЕНЦАЛЬ, Л. К. АЛТУНИНА, et al. "Preparation of Deep Eutectic Solvents for Making an Oil-Displacing Composition Based on Tetrahydroxy Alcohol, Carbamide, Quaternary Ammonium Salt, and a Surfactants." Химия в интересах устойчивого развития 32, no. 3 (2024): 343–50. http://dx.doi.org/10.15372/khur2024565.

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Синтезированы и исследованы бинарные системы глубоких эвтектических растворителей (ГЭР): “четырехатомный спирт – соль четвертичного аммониевого основания” (ГЭР1), “четырехатомный спирт – карбамид” (ГЭР2), “соль четвертичного аммониевого основания – карбамид” (ГЭР3), и тройная система “четырехатомный спирт – соль четвертичного аммониевого основания – карбамид” (ГЭР4). Эвтектические составы бинарных систем характеризуются существенно более низкими температурами застывания/плавления по сравнению с исходными компонентами. Самое низкое значение температуры среди бинарных смесей наблюдается для эвте
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22

Momin, Riyaz, Harshita Gupta, Rutu Panchal, and Priti J. Mehta. "Development of Pramipexole Hydrochloride Nanocrystals and their Characterization based on In vitro Dissolution Studies." Current Nanomedicine 10, no. 4 (2020): 391–404. http://dx.doi.org/10.2174/2468187310999201023151231.

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Background/Objectives: There are numerous unavoidable hurdles encountered by scientists to achieve an ideal drug delivery. Among them, the high-water solubility of a therapeutic molecule has been observed as a chief pausing factor that diminishes the biological stay and shortens the half-life of a drug. The ramification of this occurs that patients have to take medications multiple times in a single day to maintain the drug-plasma concentration. These consequences lead to poor pharmacological responses and ultimately do not add any significant outcomes in the betterment of patient’s health. A
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23

Ryuman, Norihiro, Nobuo Watanabe, and Takao Arai. "S-Nitrosation of Cellular Proteins by NO Donors in Rat Embryonic Fibroblast 3Y1 Cells: Factors Affecting S-Nitrosation." Oxidative Medicine and Cellular Longevity 2011 (2011): 1–8. http://dx.doi.org/10.1155/2011/450317.

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The mechanism of protein S-nitrosation in cells is not fully understood. Using rat 3Y1 cells, we addressed this issue. Among S-nitrosothiols and NO donors tested, only S-nitrosocysteine (CysNO) induced S-nitrosation when exposed in Hanks' balanced salt solution (HBSS) and not in serum-containing general culture medium. In HBSS, NO release from CysNO was almost completely abolished by sequestering metal ions with a metal chelator without affecting cellular S-nitrosation. In contrast, L-leucine, a substrate of L-type amino acid transporters (LATs), significantly inhibited S-nitrosation. The abse
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24

Mouat, M. F., A. C. Cantrell, and K. L. Manchester. "Membrane potential of rat hepatoma cells in culture: Influence of factors affecting amino acid transport." Bioscience Reports 15, no. 4 (1995): 173–84. http://dx.doi.org/10.1007/bf01540451.

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The effect has been studied of various media, hormones and of amino acids on the membrane potential of rat hepatoma cells in culture measured by microelectrode impalement. Cells in Eagle's minimal essential medium plus 5% serum had a value which varied daily from about 5–8 mV, inside negative. The membrane potential of rat hepatocytes was measured to be 8.7 ± 0.2mV, inside negative. The membrane potential of the hepatoma cells was decreased by insulin and increased by glucagon. Membrane potential was unaffected by change of medium to Hanks' or Earle's balanced salt solutions or deprivation of
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25

Kayesh, Ruhul, Vishakha Tambe, Chao Xu, and Wei Yue. "Differential Preincubation Effects of Nicardipine on OATP1B1- and OATP1B3-Mediated Transport in the Presence and Absence of Protein: Implications in Assessing OATP1B1- and OATP1B3-Mediated Drug–Drug Interactions." Pharmaceutics 15, no. 3 (2023): 1020. http://dx.doi.org/10.3390/pharmaceutics15031020.

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Impaired transport activity of hepatic OATP1B1 and OATP1B3 due to drug–drug interactions (DDIs) often leads to increased systemic exposure to substrate drugs (e.g., lipid-lowering statins). Since dyslipidemia and hypertension frequently coexist, statins are often concurrently used with antihypertensives, including calcium channel blockers (CCBs). OATP1B1/1B3-related DDIs in humans have been reported for several CCBs. To date, the OATP1B1/1B3-mediated DDI potential of CCB nicardipine has not been assessed. The current study was designed to assess the OATP1B1- and OATP1B3-mediated DDI potential
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26

Ould-Mohamed, Souhila, Sofia Gambaro, Ana Laura Ramirez-Ledesma, Carlo Paternoster, and Diego Mantovani. "Effects of Different CO2 Concentrations and Degradation Media on Static Corrosion of Commercially Pure Zinc." Crystals 13, no. 5 (2023): 753. http://dx.doi.org/10.3390/cryst13050753.

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Zn alloys have lately captivated the attention of the scientific community as possible materials for cardiovascular applications, showing a corrosion behavior and mechanical properties in between of those of Mg and Fe alloys. To better understand the different aspects of the interaction of Zn with body fluids, the basic corrosion pattern and the degradation products’ formation were investigated considering the effect of CO2 amount in the atmosphere and different pseudo-physiological media; that is Hanks’ balanced salt (HSS), Dulbecco’s phosphate-buffered saline (PSS) and physiological saline s
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27

Popov, Sergey, Nikita Paderin, Elizaveta Chistiakova, and Dmitry Ptashkin. "Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin Cross-Linked with Fe3+ Ions." International Journal of Molecular Sciences 24, no. 2 (2023): 1248. http://dx.doi.org/10.3390/ijms24021248.

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The study aims to investigate the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa ex vivo. The adhesivity of the FeP hydrogel, which was cross-linked by Fe3+ cations, exceeded that of hydrogels cross-linked by Ca2+, Zn2+, and Al3+ cations. The concentration of the cross-linking cation failed to influence the adhesion of the pectin hydrogel to the serosa. The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations. Fe3+ cations form a harder and more elastic
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28

Wells, John, and Wm V. Baird. "Alterations in Gene Expression during Exposure of Bermudagrass to Low, Non-lethal Temperatures." HortScience 32, no. 3 (1997): 534E—535. http://dx.doi.org/10.21273/hortsci.32.3.534e.

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Temperature is a limiting factor for plant growth. Warm-season turfgrasses can experience winter-kill when grown in the “transition zone.” On the other hand, when properly cold-acclimated, these same plants can withstand otherwise lethal temperatures. As part of our investigations into the biochemistry and molecular biology of cold acclimation in bermudagrass, total RNA from crowns (rhizome buds) isolated at different timepoints before and after chilling temperature exposure, was isolated by salt-buffer/phenol extraction, followed by LiCl precipitation and DNAse treatment. Differential display
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29

Wirantari, I. Dewa Ayu Putri. "Sistem Pemerintahan Ulu-Apad Berdasarkan Perda No 4 Tahun 2019 di Provinsi Bali Dalam Penanganan Covid-19 Studi Kasus : Desa Songan Kintamani." Ethics and Law Journal: Business and Notary 2, no. 1 (2024): 238–42. http://dx.doi.org/10.61292/eljbn.126.

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The Ulu-Apad leadership system in Pekraman village, which can be classified as a leadership system in the Bali Aga village, can be interpreted that Ulu is the head or leader, while Apad is the support, or buffer, so if it is interpreted that the Ulu - Apad system is a hand-in-hand leadership system. , which starts from the main to the end, means that this leadership system has a form and function that is very different from the pekraman village leadership system in general in Bali. The relationship between the Ulu-apad system and the Bali Provincial Regulation No. 4 of 2019 was based on the co
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Golob, Jonathan L., Martha M. DeMeules, Tillie Loeffelholz, et al. "2844. Butyrogenic Bacteria After Acute Graft vs. Host Disease Associate with the Development of Steroid Refractory GVHD." Open Forum Infectious Diseases 6, Supplement_2 (2019): S68. http://dx.doi.org/10.1093/ofid/ofz359.149.

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Abstract Background Steroid refractory acute graft- vs. -host-disease (GVHD) after hematopoietic cell transplantation (HCT) is highly morbid with limited treatment options. Murine studies show protection from GVHD with butyrate exposure but direct exposure of stem/progenitor cells to butyrate inhibits colonic stem cell proliferation. Methods Stool samples were collected weekly in a cohort of HCT recipients (n = 210) undergoing allogeneic transplant, and underwent 16S rRNA sequencing to determine the number and relative abundance of butyrogens. Dissociated primary human colonoid cell aggregates
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Yamada, Yusuke, Kentaro Yano, and Shunichi Fukuzumi. "Photocatalytic Hydrogen Evolution Using 9-Phenyl-10-methyl-acridinium Ion Derivatives as Efficient Electron Mediators and Ru-Based Catalysts." Australian Journal of Chemistry 65, no. 12 (2012): 1573. http://dx.doi.org/10.1071/ch12294.

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Photocatalytic hydrogen evolution has been performed by photoirradiation (λ > 420 nm) of a mixed solution of a phthalate buffer and acetonitrile (MeCN) (1 : 1 (v/v)) containing EDTA disodium salt (EDTA), [RuII(bpy)3]2+ (bpy = 2,2′-bipyiridine), 9-phenyl-10-methylacridinium ion (Ph–Acr+–Me), and Pt nanoparticles (PtNPs) as a sacrificial electron donor, a photosensitiser, an electron mediator, and a hydrogen-evolution catalyst, respectively. The hydrogen-evolution rate of the reaction system employing Ph–Acr+–Me as an electron mediator was more than 10 times higher than that employing a conve
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Krump, Eric, Serge Picard, Joseph Mancini, and Pierre Borgeat. "Suppression of Leukotriene B4 Biosynthesis by Endogenous Adenosine in Ligand-activated Human Neutrophils." Journal of Experimental Medicine 186, no. 8 (1997): 1401–6. http://dx.doi.org/10.1084/jem.186.8.1401.

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Adenosine (Ado) has been shown to suppress several functional responses of human polymorphonuclear leukocytes (PMNs). The current study investigated whether endogenous Ado regulates the biosynthesis of leukotriene (LT)B4 in ligand-stimulated PMNs. Measurements of Ado in PMN resuspended in Hanks' buffered salt solution (HBSS) or plasma showed a cell concentration– and time–dependent accumulation of the nucleoside. The removal of endogenous Ado with either Ado deaminase or the blockade of its action by the Ado A2a receptor antagonist, 8-(3-chlorostyryl) caffeine, markedly increased LTB4 biosynth
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Nuanualsuwan, Suphachai, and Dean O. Cliver. "Infectivity of RNA from Inactivated Poliovirus." Applied and Environmental Microbiology 69, no. 3 (2003): 1629–32. http://dx.doi.org/10.1128/aem.69.3.1629-1632.2003.

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ABSTRACT During inactivation of poliovirus type 1 (PV-1) by exposure to UV, hypochlorite, and heat (72°C), the infectivity of the virus was compared with that of its RNA. DEAE-dextran (1-mg/ml concentration in Dulbecco's modified Eagle medium buffered with 0.05 M Tris, pH 7.4) was used to facilitate transfecting PV-1 RNA into FRhK-4 host cells. After interaction of PV-1 RNA with cell monolayer at room temperature (21 to 22°C) for 20 min, the monolayers were washed with 5 ml of Hanks balanced salt solution. The remainder of the procedure was the same as that for the conventional plaque techniqu
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Hernandez, Josefino G. "Nasal Saline Irrigation for Sinonasal Disorders." Philippine Journal of Otolaryngology-Head and Neck Surgery 22, no. 1-2 (2007): 37–39. http://dx.doi.org/10.32412/pjohns.v22i1-2.807.

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Nasal saline irrigation is a simple easy-to-do procedure that has been used to treat sinus and nasal conditions for many years. Wingrave in his 1902 paper “The Nature of Discharges & Douches” discussed cleansing of the nasal passages as an important method of treating different nasal illnesses in the last part of the 19th century.1 Nasal saline irrigation has been used for such sinonasal conditions as atrophic rhinitis, rhinosinusitis and allergic rhinitis, among infants with nasal problems and patients who have undergone endoscopic sinus surgery. Past recommendations for use were based on
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Yang, Chen-Hsun, Hung-Ying Chang, and Peng-Wei Chu. "Comparing the Corrosion Behavior and Microstructure of a WE43 Mg Alloy in Simulated Body Fluids Under Different CO2 Conditions." ECS Meeting Abstracts MA2024-01, no. 18 (2024): 1227. http://dx.doi.org/10.1149/ma2024-01181227mtgabs.

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With the low density and high specific strength, magnesium (Mg) alloys are lightweight alloys with various applications. In addition, combined with biocompatibility, similar mechanical properties to human bones, and unique biodegradability, Mg alloys are promising biodegradable implant materials for orthopedic surgeries. However, the degradation rates of Mg alloys are too fast in physiological environments, which limits the clinical applications of Mg implants. Therefore, understanding the corrosion behavior in physiological environments is crucial for developing biodegradable Mg alloys. Durin
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Garland, Allan, Jonathan Necheles, Steven R. White, et al. "Activated eosinophils elicit substance P release from cultured dorsal root ganglion neurons." American Journal of Physiology-Lung Cellular and Molecular Physiology 273, no. 5 (1997): L1096—L1102. http://dx.doi.org/10.1152/ajplung.1997.273.5.l1096.

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This study was performed to test the hypothesis that activated eosinophils or their secretory products can directly stimulate sensory neurons to release their neuropeptides. Neurons derived from neonatal rat dorsal root ganglia (DRG), which synthesize and store sensory neuropeptides, were placed in primary cell culture and were exposed to eosinophils or their bioactive mediators. The resultant release of substance P (SP) was measured by enzyme-linked immunosorbent assay and was expressed as a percent (mean ± SE) of total neuronal SP content. Eosinophils were isolated from human volunteers with
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Agarwal, Aditi, Dinesh Rao, Sunil Panwar, and Smitha Naik. "An in vitro evaluation of St. Thomas cardioplegia solution as storage media to preserve periodontal ligament cell viability." Journal of Indian Society of Pedodontics and Preventive Dentistry 43, no. 1 (2025): 152–57. https://doi.org/10.4103/jisppd.jisppd_518_24.

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Background: The best treatment for an avulsed tooth is immediate replantation. If this is not possible, an efficient storage medium is required for the maintenance of the viability of the periodontal ligament (PDL) cells. Various synthetic and natural solutions are used as storage media for avulsed teeth. Thus, the purpose of the study was to evaluate and compare the efficacy of St. Thomas cardioplegia solution, Hanks Balanced Salt Solution (HBSS), and normal saline in maintaining the periodontal cell viability of avulsed teeth. Materials and Methods: Fifty-nine mature premolars extracted for
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S. Groeger, T. Brandau, and J. Meyle. "Development of a slow-release device for pocket disinfection." GSC Biological and Pharmaceutical Sciences 21, no. 1 (2022): 188–96. http://dx.doi.org/10.30574/gscbps.2022.21.1.0198.

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Polyhexanide (polyhexamethylene biguanide) (PHMB) is a polymeric substance that is broadly used as disinfectant and antiseptic. It was shown to be suitable for clinical use in critically colonized or infected acute and chronic wounds since it exhibits a broad antimicrobial spectrum combined with good cell and tissue tolerability and low risk of contact sensitization. Furthermore a wound healing promoting effect was reported. The aim of this work was to develop a PHMB containing slow release device for sustainable disinfection of bacterial infected periodontal pockets and other colonized niches
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S., Groeger, Brandau T., and Meyle J. "Development of a slow-release device for pocket disinfection." GSC Biological and Pharmaceutical Sciences 21, no. 1 (2022): 188–96. https://doi.org/10.5281/zenodo.7649014.

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Polyhexanide (polyhexamethylene biguanide) (PHMB) is a polymeric substance that is broadly used as disinfectant and antiseptic. It was shown to be suitable for clinical use in critically colonized or infected acute and chronic wounds since it exhibits a broad antimicrobial spectrum combined with good cell and tissue tolerability and low risk of contact sensitization. Furthermore a wound healing promoting effect was reported. The aim of this work was to develop a PHMB containing slow release device for sustainable disinfection of bacterial infected periodontal pockets and other colonized niches
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Yamamoto, Takashi, and Yasuaki Einaga. "Molecularly Modified Diamond Electrodes." ECS Meeting Abstracts MA2025-01, no. 48 (2025): 2481. https://doi.org/10.1149/ma2025-01482481mtgabs.

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Molecular modification of the electrode surface is an important technique in various fields ranging from analytical chemistry to molecular electronics. For example, in the field of analytical chemistry, highly sensitive immunosensors have been reported where the electrode surfaces were modified with an antibody or a protein. In the field of molecular electronics, excellent rectifiers and organic light-emitting diodes have been achieved by immobilizing self-assembled monolayers on the electrode surface. Functional molecules can be modified via formation of C−C bonds when carbon materials are us
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Green, Michael, Lindsey Miles, and Stephen Hawley. "Discriminating between Cell Surface and Intracellular Plasminogen-binding Proteins: Heterogeneity in Profibrinolytic Plasminogen-binding Proteins on Monocytoid Cells." Thrombosis and Haemostasis 84, no. 11 (2000): 882–90. http://dx.doi.org/10.1055/s-0037-1614132.

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SummaryWhen plasminogen binds to cell surfaces, its activation is markedly enhanced compared to soluble plasminogen. Although several distinct molecules may contribute to plasminogen binding to a given cell type, the subset of plasminogen receptors responsible for enhancing plasminogen activation expose a carboxyl-terminal lysine on the cell surface and are sensitive to proteolysis by carboxypeptidase B (CpB). To distinguish this subset of plasminogen receptors from plasminogen-binding proteins that are not profibrinolytic, we treated intact U937 monocytoid cells and peripheral blood monocytes
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Anjum, Adeel, Moshin Masood, Mudasar Hussain, et al. "Isolation and Identification of Bacteria from the Oral Cavity of Monitor Lizard (Varanus bengalensis)." Advances in Applied Biological Research 1, no. 1 (2024): 32–39. https://doi.org/10.48165/aabr.2024.1.05.

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The current research was carried out in the Kasur district, Punjab, Pakistan with the duration of July 2021 to June 2022. The purpose of study was to isolate and identify the bacterial community in the oral cavity of monitor lizard (Varanus bengalensis). As monitor lizards are ectotherm reptiles so they spent most of their winter season from November to March in hibernation. So, sampling is done before and after the hibernation period. Ten specimens were captured from the study area by the direct encounter and also using hand nets and pitfall traps. The collected samples were transferred to th
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Ariga, Kotoko, Seiya Tsujimura, and Tsutomu Mikawa. "Evaluation of Enzyme Cascade Electrode Reactions at Nanoscale Area." ECS Meeting Abstracts MA2024-02, no. 54 (2024): 3675. https://doi.org/10.1149/ma2024-02543675mtgabs.

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Introduction Enzymes are promising catalysts with high selectivity and low environmental load. Especially, redox enzymes are attracting attentions because of their applicability to electrodes for biosensors and biofuel cells. For example, wearable lactate biosensors which can detect lactate in sweat and use the reaction to drive itself have been developed recently[1]. Various biosensors are already available today. However, most of them use single-enzyme electrodes. Compared to single-enzyme electrodes, multi-enzyme electrodes are expected to improve the performance of conventional biosensors
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Bagha, Pedram Sotoudeh, Carlo Paternoster, Mehrdad Khakbiz, Saeed Sheibani, Navid Gholami, and Diego Mantovani. "Surface Modification of an Absorbable Bimodal Fe-Mn-Ag Alloy by Nitrogen Plasma Immersion Ion Implantation." Materials 16, no. 3 (2023): 1048. http://dx.doi.org/10.3390/ma16031048.

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Recently, Fe-Mn-based alloys have been increasingly catching the attention of the scientific community, because of their tunable and outstanding mechanical properties, and suitable degradation behavior for biomedical applications. In spite of these assets, their corrosion rate (CR) is, in general, too low to satisfy the requirements that need to be met for cardiovascular device applications, such as stents. In fact, the CR is not always the same for all of the degradation stages of the material, and in addition, a finely tuned release rate, especially during the first steps of the corrosion pa
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45

Francis, Richard. "Assessment of liquid media requirements for storing and evaluating respiratory cilia motility." PeerJ 13 (March 27, 2025): e19191. https://doi.org/10.7717/peerj.19191.

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Mucociliary clearance is critical for maintaining normal lung function. Respiratory cilia which drive mucociliary clearance are commonly studied by measuring cilia beat frequency (CBF). There is currently significant variation within the literature regarding what is a normal value for CBF, this may be due in part to the large variety of liquid media used to suspend, maintain, and image ciliated cells. This study aimed to conduct a thorough examination to assess how media choice influences respiratory cilia motility. To accomplish this, Adult C57/BL6 mouse trachea samples were incubated in eigh
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Cini, Renzo, Rebecca Pogni, Riccardo Basosi, et al. "Oxygen Radical Scavenger Activity, EPR, NMR, Molecular Mechanics and Extended-Hückel Molecular Orbital Investigation of the Bis(Piroxicam)Copper(II) Complex." Metal-Based Drugs 2, no. 1 (1995): 43–56. http://dx.doi.org/10.1155/mbd.1995.43.

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The oxygen radical scavenger activity (ORSA) of [CuII(Pir)2] (HPir = Piroxicam = 4-hydroxy -2- methyl -N-2- pyridyl -2H- 1,2-benzothiazine -3- carboxamide 1,1-dioxide) was determined by chemiluminescence of samples obtained by mixing human neutrophils (from healthy subjects) and [CuII(Pir)2(DMF)2] (DMF = N,N -dimethylformammide) in DMSO/GLY/PBS (2:1:2, v/v) solution (DMSO = dimethylsulfoxide, GLY = 1,2,3-propantriol, PBS = Dulbecco’s buffer salt solution). The ratio of the residual radicals, for the HPir (1.02·10−4M) and [CuII(Pir)2(DMF)2] (1.08·10−5M) /HPir (8.01·10−5M) systems was higher tha
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Slipetz, Deborah, Stephanie Buchanan, Cameron Mackereth та ін. "Sequestration and phosphorylation of the prostaglandin E2 EP4 receptor: dependence on the C-terminal tail33Abbreviations: PGE2, prostaglandin E2, GRK, G-protein coupled receptor kinase; PKA, protein kinase A; PKC, protein kinase C; PCR, polymerase chain reaction; DMEM, Dulbecco’s modified Eagle’s medium; PAS, protein A Sepharose; ECL, electrochemiluminescence; BCA, 2-bicinchoninic acid; IR, immunoreactivity; HBSS, Hanks’ buffered salt solution; TBS, Tris-buffered saline; cAMP, cyclic AMP; PMA, phorbol 12-myristate 13 acetate; and β2-AR, β2-adrenergic receptor." Biochemical Pharmacology 62, № 8 (2001): 997–1012. http://dx.doi.org/10.1016/s0006-2952(01)00742-0.

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48

Ortiz, I., M. Felix, H. Resende, C. Love, and K. Hinrichs. "120 Use of fixable dyes to analyze equine sperm membrane integrity and acrosome reaction after A23187 treatment." Reproduction, Fertility and Development 32, no. 2 (2020): 187. http://dx.doi.org/10.1071/rdv32n2ab120.

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Conventional IVF is not successful in the horse, and current work is focused on factors affecting sperm capacitation in this species. Challenges arise in assessing equine sperm incubated in media containing capacitation promotors, as some of these factors cause sperm head-to-head binding (aggregation). Our preliminary microscopic findings showed that sperm aggregates are largely of viable sperm, whereas nonviable sperm individualize. Thus, data obtained using technologies that analyse only individual cells and gate out aggregates, such as flow cytometry, may not accurately represent the study
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Ranaldi, Giulia, Iolanda Marigliano, Isabella Vespignani, Giuditta Perozzi, and Yula Sambuy. "The effect of chitosan and other polycations on tight junction permeability in the human intestinal Caco-2 cell line1 1Abbreviations: AP = apical; BL = basolateral; 2-DOG = 2-deoxyglucose; HBSS = Hanks balanced salt solution; FITC = fluorescein isothiocyanate; HEPES = N-2-hydroxyethyl piperazine-N-4-butanesulfonic acid; HMW = high molecular weight; LMW = low molecular weight; MES = morpholinoethane sulfonic acid; PBS+ = phosphate buffered saline; PEI = polyethylenimine; TEER = trans-epithelial electrical resistance; TRITC = tetramethylrodamine isothiocyanate." Journal of Nutritional Biochemistry 13, no. 3 (2002): 157–67. http://dx.doi.org/10.1016/s0955-2863(01)00208-x.

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Adeoti, Olatunde Micheal, Abidemi Hawawu Bello, Olajumoke Elisabeth Adedokun, Kafilat Adenike Komolafe, David Ademola Adesina, and Opeyemi Joy Olaoye. "Distinctive Molecular typing of 16S rRNA of Bacillus species isolated from farm settlement." International Journal of Immunology and Microbiology 1, no. 1 (2021): 10–15. http://dx.doi.org/10.55124/ijim.v1i1.55.

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Introduction: There are numerous methods of isolating and detecting organisms that are similar and closely related; one of the most reliable method is molecular typing of 16S rRNA. Apart from being omnipresent as a multigene family, or operons; it is evolutionarily stable; the 16S rRNA gene (1,500 bp) is large enough for informatics purposes.
 Materials and Method: This study employed molecular sequencing of 16S rRNA by Sanger method to reveal the specific organisms’ nucleotides and blasting (BLASTn) to show the similarities between the resulting organisms and existing organisms. The 16S
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