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Coats, Vanessa, Christa R. Schwintzer, and John D. Tjepkema. "Truncated hemoglobins in Frankia CcI3: effects of nitrogen source, oxygen concentration, and nitric oxide." Canadian Journal of Microbiology 55, no. 7 (July 2009): 867–73. http://dx.doi.org/10.1139/w09-042.
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Frankia strain CcI3 produces 2 truncated hemoglobins, HbN and HbO. Using ion-exchange chromatography, we characterized the expression of the relative amounts of HbN and HbO in −N (nitrogen-fixing) cultures and +N (nitrogen-supplemented) cultures. The −N cultures maintained an approximately constant ratio of HbO to HbN throughout the life of the culture, with HbO constituting 80%–85% of the total hemoglobin produced. In contrast, in +N cultures, HbN was observed to increase over time and HbO decreased. Total hemoglobin as a fraction of total protein was approximately constant throughout the growth phase in −N cultures, while it decreased somewhat in +N cultures. Subjecting −N cultures to a NO generator resulted in increased production of HbN, relative to the controls. Nitrite accumulated in +N cultures, but not in −N cultures. This suggests that the greater amount of HbN in +N cultures might be due to NO produced by the reduction of nitrite. The effects of O2 concentration were determined in +N cultures. Cultures grown in 1% O2 produced about 4 times more HbO than cultures grown in 20% O2. Overall, these results provide evidence for a role of HbN in NO oxidation and for a role of HbO in adaptation to low oxygen concentrations.
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Mura, David. "HBO." WSQ: Women's Studies Quarterly 47, no. 1-2 (2019): 261. http://dx.doi.org/10.1353/wsq.2019.0021.
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Sexton, Max. "HBO Effect." CINEJ Cinema Journal 6, no. 1 (September 14, 2017): 135–39. http://dx.doi.org/10.5195/cinej.2017.148.
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Osinski, Steve. "HBO EDUCATION." American Journal of Nursing 102, no. 4 (April 2002): 14. http://dx.doi.org/10.1097/00000446-200204000-00009.
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Addis, Victoria. "Deadwood, HBO." Middle West Review 4, no. 2 (2018): 155–59. http://dx.doi.org/10.1353/mwr.2018.0038.
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Noble, Randolph, and Richard Grossman. "Therapeutic HBO." Journal of Burn Care & Rehabilitation 9, no. 6 (November 1988): 581–83. http://dx.doi.org/10.1097/00004630-198811000-00001.
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Nelson, Robin. "HBO PREMIUM." New Review of Film and Television Studies 5, no. 1 (April 2007): 25–40. http://dx.doi.org/10.1080/17400300601140159.
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Rockswold, Sarah B., Gaylan L. Rockswold, Janet M. Vargo, Carla A. Erickson, Richard L. Sutton, Thomas A. Bergman, and Michelle H. Biros. "Effects of hyperbaric oxygenation therapy on cerebral metabolism and intracranial pressure in severely brain injured patients." Journal of Neurosurgery 94, no. 3 (March 2001): 403–11. http://dx.doi.org/10.3171/jns.2001.94.3.0403.
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Object. Hyperbaric oxygenation (HBO) therapy has been shown to reduce mortality by 50% in a prospective randomized trial of severely brain injured patients conducted at the authors' institution. The purpose of the present study was to determine the effects of HBO on cerebral blood flow (CBF), cerebral metabolism, and intracranial pressure (ICP), and to determine the optimal HBO treatment paradigm. Methods. Oxygen (100% O2, 1.5 atm absolute) was delivered to 37 patients in a hyperbaric chamber for 60 minutes every 24 hours (maximum of seven treatments/patient). Cerebral blood flow, arteriovenous oxygen difference (AVDO2), cerebral metabolic rate of oxygen (CMRO2), ventricular cerebrospinal fluid (CSF) lactate, and ICP values were obtained 1 hour before and 1 hour and 6 hours after a session in an HBO chamber. Patients were assigned to one of three categories according to whether they had reduced, normal, or raised CBF before HBO. In patients in whom CBF levels were reduced before HBO sessions, both CBF and CMRO2 levels were raised 1 hour and 6 hours after HBO (p < 0.05). In patients in whom CBF levels were normal before HBO sessions, both CBF and CMRO2 levels were increased at 1 hour (p < 0.05), but were decreased by 6 hours after HBO. Cerebral blood flow was reduced 1 hour and 6 hours after HBO (p < 0.05), but CMRO2 was unchanged in patients who had exhibited a raised CBF before an HBO session. In all patients AVDO2 remained constant both before and after HBO. Levels of CSF lactate were consistently decreased 1 hour and 6 hours after HBO, regardless of the patient's CBF category before undergoing HBO (p < 0.05). Intracranial pressure values higher than 15 mm Hg before HBO were decreased 1 hour and 6 hours after HBO (p < 0.05). The effects of each HBO treatment did not last until the next session in the hyperbaric chamber. Conclusions. The increased CMRO2 and decreased CSF lactate levels after treatment indicate that HBO may improve aerobic metabolism in severely brain injured patients. This is the first study to demonstrate a prolonged effect of HBO treatment on CBF and cerebral metabolism. On the basis of their data the authors assert that shorter, more frequent exposure to HBO may optimize treatment.
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Shyu, Kou-Gi, Bao-Wei Wang, and Hang Chang. "Hyperbaric oxygen activates discoidin domain receptor 2 via tumour necrosis factor-α and the p38 MAPK pathway to increase vascular smooth muscle cell migration through matrix metalloproteinase 2." Clinical Science 116, no. 7 (March 2, 2009): 575–83. http://dx.doi.org/10.1042/cs20080215.
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DDR2 (discoidin domain receptor 2) regulates collagen turnover mediated by SMCs (smooth muscle cells) in atherosclerosis. HBO (hyperbaric oxygen) has been used in medical practice; however, the molecular mechanism of the beneficial effects of HBO is poorly understood. Furthermore, the effect of HBO on DDR2 has not been reported previously. In the present study, we investigated the cellular and molecular mechanisms of DDR2 regulation by HBO in VSMCs (vascular SMCs). Cells were exposed to 2.5 ATA (atmosphere absolute) of oxygen in a hyperbaric chamber. DDR2 protein (3.63-fold) and mRNA (2.34-fold) expression were significantly increased after exposure to 2.5 ATA HBO for 1 h. Addition of SB203580 and p38 MAPK (mitogen-activated protein kinase) siRNA (small interfering RNA) 30 min before HBO inhibited the induction of DDR2 protein. HBO also significantly increased DNA–protein binding activity of Myc/Max. Addition of SB203580 and an anti-TNF-α (tumour necrosis factor-α) monoclonal antibody 30 min before HBO abolished the DNA–protein binding activity induced by HBO. HBO significantly increased the secretion of TNF-α from cultured VSMCs. Exogenous addition of TNF-α significantly increased DDR2 protein expression, whereas anti-TNF-α and anti-(TNF-α receptor) antibodies blocked the induction of DDR2 protein expression. HBO significantly increased VSMC migration and proliferation, whereas DDR2 siRNA inhibited the migration induced by HBO. HBO increased activated MMP2 (matrix metalloproteinase 2) protein expression, and DDR2 siRNA abolished the induction of activated MMP2 expression induced by HBO. In conclusion, HBO activates DDR2 expression in cultured rat VSMCs. HBO-induced DDR2 is mediated by TNF-α and at least in part through the p38 MAPK and Myc pathways.
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Wu, Zong-Sheng, Jing-Jou Lo, Sheng-Hua Wu, Chau-Zen Wang, Rong-Fu Chen, Su-Shin Lee, Chee-Yin Chai, and Shu-Hung Huang. "Early Hyperbaric Oxygen Treatment Attenuates Burn-Induced Neuroinflammation by Inhibiting the Galectin-3-Dependent Toll-Like Receptor-4 Pathway in a Rat Model." International Journal of Molecular Sciences 19, no. 8 (July 27, 2018): 2195. http://dx.doi.org/10.3390/ijms19082195.
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Hyperbaric oxygen (HBO) treatment has been proven to decrease neuroinflammation in rats. This study aimed to determine the potential mechanism underlying the anti-inflammatory effects of HBO treatment on burn-induced neuroinflammation in rats. Thirty-six adult male Sprague-Dawley (SD) rats were randomly assigned to the following six groups (n = 6 per group): (1) sham burn with sham HBO treatment; (2) sham burn with HBO treatment; (3) burn with one-week sham HBO treatment; (4) burn with two-week sham HBO treatment; (5) burn with one-week HBO treatment; and (6) burn with two-week HBO treatment. SD rats that received third-degree burn injury were used as a full-thickness burn injury model. Subsequently, we analyzed the expression of proteins involved in the galectin-3 (Gal-3)-dependent Toll-like receptor-4 (TLR-4) pathway through enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC) analysis, and Western blotting. A behavior test was also conducted, which revealed that HBO treatment significantly suppressed mechanical hypersensitivity in the burn with HBO treatment group compared to the burn with sham HBO treatment group (p < 0.05). ELISA results showed that tumor necrosis factor α (TNF-α) and interleukin 1 beta (IL-1β) levels in the dorsal horn of the spinal cord and the skin significantly decreased in the burn with HBO treatment group compared with the burn with sham HBO treatment group (p < 0.05). Western blotting results demonstrated that HBO treatment significantly reduced the expression of Gal-3 and TLR-4 in the dorsal horn of the spinal cord in the burn with HBO treatment group compared with the burn with sham HBO treatment group (p < 0.05). IHC analysis showed that the expression of Gal-3, TLR-4, CD68 and CD45 in the dorsal horn of the spinal cord was significantly lower in the burn with HBO treatment group than in the burn with sham HBO treatment group (p < 0.05), and the expression of CD68 and macrophage migration inhibitory factor (MIF) in the right hind paw skin was significantly lower. The expression of vimentin and fibroblast growth factor in the right hind paw skin was significantly higher after HBO treatment (p < 0.05). This study proved that early HBO treatment relieves neuropathic pain, inhibits the Gal-3-dependent TLR-4 pathway, and suppresses microglia and macrophage activation in a rat model.
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Lee, Ying-Sheng, Chung-Ching Chio, Ching-Ping Chang, Liang-Chao Wang, Po-Min Chiang, Kuo-Chi Niu, and Kuen-Jer Tsai. "Long Course Hyperbaric Oxygen Stimulates Neurogenesis and Attenuates Inflammation after Ischemic Stroke." Mediators of Inflammation 2013 (2013): 1–13. http://dx.doi.org/10.1155/2013/512978.
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Several studies have provided evidence with regard to the neuroprotection benefits of hyperbaric oxygen (HBO) therapy in cases of stroke, and HBO also promotes bone marrow stem cells (BMSCs) proliferation and mobilization. This study investigates the influence of HBO therapy on the migration of BMSCs, neurogenesis, gliosis, and inflammation after stroke. Rats that sustained transient middle cerebral artery occlusion (MCAO) were treated with HBO three weeks or two days. The results were examined using a behavior test (modified neurological severity score, mNSS) and immunostaining to evaluate the effects of HBO therapy on migration of BMSCs, neurogenesis, and gliosis, and expression of neurotrophic factors was also evaluated. There was a lower mNSS score in the three-week HBO group when compared with the two-day HBO group. Mobilization of BMSCs to an ischemic area was more improved in long course HBO treatments, suggesting the duration of therapy is crucial for promoting the homing of BMSCs to ischemic brain by HBO therapies. HBO also can stimulate expression of trophic factors and improve neurogenesis and gliosis. These effects may help in neuronal repair after ischemic stroke, and increasing the course of HBO therapy might enhance therapeutic effects on ischemic stroke.
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Lin, Tara L., Amanda Wise, Abigale Berry, Joseph Fontes, Partha Kasturi, Brea C. Lipe, and Omar S. Aljitawi. "Hyperbaric Oxygen Increases Sensitivity to Chemotherapy in Acute Leukemia." Blood 126, no. 23 (December 3, 2015): 1399. http://dx.doi.org/10.1182/blood.v126.23.1399.1399.
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Abstract Introduction: Chemotherapy resistance remains a significant challenge in the treatment of adults with Acute Myeloid Leukemia (AML). Although new targets and novel agents are under clinical investigation, the majority of AML patients are treated with conventional induction chemotherapy consisting of an anthracycline and cytarabine, with little modification in over 40 years. Insights into the biology of resistance to cytarabine and anthracyclines may suggest novel strategies in the treatment of AML. Hyperbaric oxygen (HBO) treatment is commonly used in the treatment of decompression sickness from scuba diving, wound healing and infections. 100% oxygen is administered at increased pressure. Limited published data suggest a role for HBO to cause in vitro apoptosis of leukemia cell lines, but no mechanism has been described. We examined the role of HBO in AML sensitivity to chemotherapy, reactive oxygen species (ROS) generation, glycolytic pathway activation and gene expression. Methods: Human AML cell lines HL-60 and MV-411 were treated with escalating doses of cytarabine or daunorubicin as single agents followed by HBO treatment for 2 hours or normobaric conditions. HBO treatment consisted of exposure to 100% oxygen at 2.5 atmosphere absolutes for a total of 2 hours in a specifically designed in vitro HBO chamber. At 24 hours after chemotherapy dosing, leukemia cells were analyzed for proliferation using Cell Titer 96 Aqueous MTS assay (Promega). Apoptosis was measured using Annexin V-FITC and analyzed by flow cytometry. In order to understand potential mechanisms of HBO activity on leukemia cells, studies of HBO-treated cells without use of chemotherapy were also performed. CellROX Oxidative Stress assay (Life Technologies) was performed at 24 hours. CellROX Green and Deep Red reagents measured ROS level in the DNA and cytoplasm. RNA was isolated and real-time PCR was performed using CFX384 Real-time PCR system using primer sets specific for human hexokinase2 and phosphofructokinase to examine the effects of HBO on glycolysis. Gene expression profiling was performed using the Human Transcriptome Array 2.0 (Affymetrix) and examined differences in gene expression following HBO treatment of HL-60 cells and bone marrow from an AML patient. Results: HBO-treated leukemia cells had increased sensitivity to cytarabine (Table 1, Figure 1) (MV411 response to cytarabine: IC50 2649nM without HBO treatment, 1921nM with HBO, p=0.02; HL60 response to cytarabine: linear response without HBO, IC50 1625nM with HBO, p<0.035). Decreased proliferation was also seen with HBO-treated cells in response to daunorubicin but was not statistically significant (Table 1). Similarly, apoptosis from cytarabine in HL-60 cells was increased with HBO treatment (p<0.02), but the results with daunorubicin were not significant. Significant increases in ROS levels were observed at 24 hours post-HBO for both cell lines when compared to non-treated cells (HL60 DNA p<0.0001, HL60 cytoplasm p<0.01, MV411 DNA p<0.01, MV411 cytoplasm p<0.01). Glycolysis pathway components human hexokinase 2 and phosphofructokinase were upregulated in HL60 and MV411 cells treated with HBO compared to controls. Gene expression profiling showed that approximately 200 genes in coding regions were upregulated in HBO-treated HL-60 cells; specific piRNA clusters were downregulated in both AML cell line HL60 and a bone marrow sample from a patient with relapsed AML when treated with HBO. Conclusions: HBO treatment significantly increases the sensitivity to cytarabine AML cell lines in vitro. Increased ROS, upregulation of glycolysis-related enzymes and changes in gene expression profiling provide insight into the potential mechanisms of HBO enhanced chemotherapy sensitivity. Given the safety and commonplace use of HBO for other indications, and the significant challenge of chemotherapy resistance, it is important to pursue further studies to understand the biology of HBO treatment in AML. Table 1. IC50 (nM) of chemotherapy agents in AML cell lines with and without co-treatment with HBO. Cytarabine Daunorubicin no HBO HBO p-value no HBO HBO p-value MV411 2649 1921 0.0214 709.1 641.1 ns HL60 * 1625 0.0346 639 406.5 ns * linear response, no IC50 available Figure 1. Increased sensitivity to cytarabine in AML cell lines following treatment with HBO. Figure 1. Increased sensitivity to cytarabine in AML cell lines following treatment with HBO. Disclosures No relevant conflicts of interest to declare.
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Juskovic, Aleksandar, Marina Nikolic, Biljana Ljujic, Aleksandar Matic, Vladimir Zivkovic, Ksenija Vucicevic, Zoran Milosavljevic, et al. "Effects of Combined Allogenic Adipose Stem Cells and Hyperbaric Oxygenation Treatment on Pathogenesis of Osteoarthritis in Knee Joint Induced by Monoiodoacetate." International Journal of Molecular Sciences 23, no. 14 (July 12, 2022): 7695. http://dx.doi.org/10.3390/ijms23147695.
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The beneficial effects of HBO in inflammatory processes make it an attractive type of treatment for chronic arthritis. In addition, the effects of combination therapy based on adipose stem cells and HBO on OA progression have not been fully investigated. The current study explored the efficacy of intra-articular injection of allogeneic adipose-derived mesenchymal stem cells (ADMSCs) combined with hyperbaric oxygenation treatment (HBO) in a rat osteoarthritis (OA) model. The rat OA model was induced by intra-articular injection of monoiodoacetate (MIA) and 7 days after application of MIA rats were divided into five groups: healthy control (CTRL), osteoarthritis (OA), ADMSCs (ADS), the HBO+ADS21day and HBO+ADS28day groups. A single dose of 1 × 106 allogeneic ADMSCs suspended in sterile saline was injected into the knee joint alone or in combination with HBO treatment. Rats were sacrificed at 3 or 4 weeks after MIA injection. Treatment outcomes were evaluated by radiographic, morphological and histological analysis and by specific staining of articular cartilage. We also measured the level of inflammatory and pro/antioxidative markers. We confirmed that combined treatment of ADMSCs and HBO significantly improved the regeneration of cartilage in the knee joint. Rtg score of knee joint damage was significantly decreased in the HBO+ADS21day and HBO+ADS28day groups compared to the OA. However, the positive effect in the HBO+ADS28day group was greater than the HBO+ADS21day group. The articular cartilage was relatively normal in the HBO+ADS28day group, but moderate degeneration was observed in the HBO+ADS21day compared to the OA group. These findings are in line with the histopathological results. A significantly lower level of O2−. was observed in the HBO+ADS28day group but a higher NO level compared to the HBO+ADS21day group. Moreover, in the HBO+ADS28day group significantly higher concentrations of IL-10 were observed but there was no significant difference in proinflammatory cytokine in serum samples. These results indicate that a single intra-articular injection of allogeneic ADMSCs combined with HBO efficiently attenuated OA progression after 28 days with greater therapeutic effect compared to alone ADMSCs or after 3 weeks of combined treatment. Combined treatment might be an effective treatment for OA in humans.
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Li, Chaozheng, Yonggang Yang, Chi Ma, and Yufang Liu. "Effect of amino group on the excited-state intramolecular proton transfer (ESIPT) mechanisms of 2-(2′-hydroxyphenyl)benzoxazole and its amino derivatives." RSC Advances 6, no. 6 (2016): 5134–40. http://dx.doi.org/10.1039/c5ra23261a.
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The electronic density redistributes and it migrates in opposite directions for HBO when compared to those of 5A-HBO and 6A-HBO. The amino group in the HBO framework can change the behavior of the intramolecular hydrogen bonds.
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Han, Guang, Lu Li, and Ling-xin Meng. "Effects of Hyperbaric Oxygen on Pain-Related Behaviours and Nitric Oxide Synthase Expression in a Rat Model of Neuropathic Pain." Pain Research and Management 18, no. 3 (2013): 137–41. http://dx.doi.org/10.1155/2013/147043.
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BACKGROUND: Neuropathic pain is complex, and a satisfactory therapeutic method of treatment has yet to be developed; therefore, finding a new and effective therapeutic method is an important issue in the field of neuropathic pain.OBJECTIVE: To determine the effects of hyperbaric oxygen (HBO) on pain-related behaviours and nitric oxide synthase (NOS) expression in a rat model of neuropathic pain.METHODS: Forty male Sprague Dawley rats were randomly divided into five groups (eight rats per group) including control, sham operation, sciatic nerve with chronic constriction injury (CCI), HBO pretreatment (pre-HBO) and HBO post-treatment (post-HBO) groups. Pain-related behaviours and NOS expression in the spinal cord were compared among the five groups.RESULTS: Compared with the CCI group, the mechanical withdrawal threshold was significantly increased and thermal withdrawal latency was significantly extended in the pre-HBO and post-HBO groups (all P<0.05). After CCI, expression of spinal neuronal NOS and inducible NOS were increased. Expression of spinal neuronal NOS and inducible NOS were significantly decreased in the pre-HBO and post-HBO groups compared with the CCI group (all P<0.05). Spinal eNOS expression changed very little.DISCUSSION: HBO has been used as an effective and noninvasive method for the treatment of spinal cord injuries and high-altitude sickness, and in immunosuppression and stem-cell research; however, it has yet to be applied to the treatment of neuropathic pain. The present study indicated that HBO effectively increased mechanical withdrawal threshold and thermal withdrawal latency, demonstrating that HBO has therapeutic effects on neuropathic pain.CONCLUSION: HBO inhibits pain in rats with CCI through the regulation of spinal NOS expression.
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Sula, B., C. Ekinci, H. Uçak, D. Uçmak, ZM Akkurt, A. Aktaş, M. Arica, and E. Deveci. "Effects of hyperbaric oxygen therapy on rat facial skin." Human & Experimental Toxicology 35, no. 1 (March 4, 2015): 35–40. http://dx.doi.org/10.1177/0960327115575758.
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Introduction: We used immunohistochemistry to investigate the histopathologic effects of hyperbaric oxygen (HBO) on the facial skin of rats. Material and methods: Rats in the HBO group ( n = 6) were placed in a 20 L HBO chamber at 2.5 atmospheres absolute at 25–26°C with 100% oxygen for 90 min/day for 7 days. Following euthanasia, sections of facial skin were removed for examination. Results: Epidermal hyperplasia and degeneration, basal-cell hypertrophy, subepithelial fibrosis, and increased connective tissue were observed in the HBO group. E-cadherin expression was reduced in the epidermis, hair follicles, and sebaceous glands in HBO-treated rats relative to control animals. HBO treatment was associated with vimentin immunoreactivity in fibroblasts, endothelial cells, and the bulbus pilorum of a subset of hair follicles. It also resulted in increased type IV collagen expression within the connective tissue in the hair follicles and sebaceous glands. Conclusion: The HBO group demonstrated epidermal hyperplasia and degeneration, basal-cell hypertrophy, and subepithelial fibrosis. In addition, HBO decreased E-cadherin expression, which suggests that HBO may impair intracellular adhesion. Expression of vimentin and type IV collagen was also observed in the dermis. Increased connective tissue, hemorrhage, and mononuclear cell infiltration were observed in the dermis of HBO-treated animals. Thus, HBO has effects on the structures of the epidermis and dermis.
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Rocha, Flaviana Soares, Layra Gabriella Pereira de Rezende, Danyella Carolyna Soares dos Reis, Gustavo Davi Rabelo, Letícia de Souza Castro Filice, Darceny Zanetta-Barbosa, and Paula Dechichi. "Influência da oxigenação hiperbárica no reparo ósseo e organização do colágeno após radioterapia." Research, Society and Development 9, no. 11 (November 20, 2020): e4339119948. http://dx.doi.org/10.33448/rsd-v9i11.9948.
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O presente estudo teve como objetivo avaliar os efeitos da HBO no reparo ósseo após radiação ionizante, por meio da histomorfometria, tomografia computadorizada e microscopia de polarização. Vinte ratos Wistar machos foram usados. Uma dose de radiação (30 Gy) foi administrada na perna esquerda em todos os animais, e após 30 dias, defeitos ósseos foram criados em ambos os fêmures. Em seguida, 10 animais receberam sessões diárias de HBO (2,5 ATA por 90 minutos), e todos os animais foram sacrificados 5 ou 7 dias após a cirurgia. Os fêmures foram separados em 4 grupos (n = 5) para cada intervalo de tempo de eutanásia: Controle (fêmur direito: não irradiado e não HBO), RXT (fêmur esquerdo: irradiado e não HBO), HBO (fêmur direito: não-irradiado com HBO), RXT + HBO (fémur esquerdo: irradiado com HBO). A cortical óssea e os defeitos foram avaliados. Os dados foram analisados por meio dos testes de Kolmogorov-Smirnov, teste t não pareado e ANOVA com correção de Bonferroni. Valores maiores de HU e neoformação óssea foram observados nos grupos Controle e HBO, com melhora do reparo. O grupo HBO apresentou birrefringência de colágeno predominantemente amarela/laranja/vermelha na área do defeito. A HBO melhorou a reparação óssea em condições fisiológicas, com aumento dos vasos sanguíneos e formação óssea. No entanto, não foi eficiente em melhorar o reparo e a orientação do colágeno no osso após altas doses de radiação ionizante.
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Savilov, P. N. "On the question of studying hyperoxic sanogenesis SARS-CoV-2-associated pneumonia." Marine Medicine 8, no. 1 (April 11, 2022): 7–19. http://dx.doi.org/10.22328/2413-5747-2022-8-1-7-19.
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The article is devoted to the analysis of literature data on the use of hyperbaric oxygenation (HBO) in patients with COVID-19, complicated by the development of SARS-CoV-2-associated pneumonia to build a hypothesis about possible mechanisms of therapeutic action of hyperbaric oxygen (HBO2) in this pathology. The expediency of using «soft» (1.3–2.0 attacks, 40–60 min) HBO modes in SARS-CoV-2-associated pneumonia is substantiated. Several possible mechanisms of elimination of HBO2 violation of lung gas exchange function in SARS-CoV-2-associated pneumonia are considered. Firstly, hyperoxic stimulation of diaphragm contraction. Secondly, the inhibitory effect of HBO2 on the development of interstitial and alveolar edema in the lungs. Thirdly, elimination of HBO2 stimulating effect of thrombin and fibrinogen on contractility of pulmonary capillary endotheliocytes. Fourth, regulation of HBO2 metabolism of fibronectin, thromboplastin, von Willibrant factors, and platelet activation factor in the wall of pulmonary capillaries. As a result, its thrombogenic activity increases in this pathology.
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ETLÝK, Ö., V. SAÐMANLIGÝL, Ý. PÝÞKÝN, and A. Tomur. "THE EFFECTS OF CYCLOPHOSPHAMIDE AND ITS UROPROTECTIVE AGENTS, MESNA AND HYPERBARIC OXYGEN, ON URINARY BLADDER MOTILITY IN GUINEA PIGS." Acta Veterinaria Hungarica 47, no. 4 (November 1, 1999): 451–60. http://dx.doi.org/10.1556/avet.47.1999.4.5.
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The aim of this research was to observe the effects of cyclophosphamide and its uroprotective agents, mesna and hyperbaric oxygen (HBO), on the motility of urinary bladder muscle in guinea pigs. In the experimental groups, mesna and cyclophosphamide were intraperitoneally injected at a dose of 21.5 mg/kg and 68.1 mg/kg, respectively. For the combination of mesna and cyclophosphamide, one dose of mesna was injected 20 min before cyclophosphamide administration and three additional injections of mesna were repeated every three hours. A total of 8 HBO exposures were performed at 2.8 ATA for 90 min twice daily for another experimental group. In the HBO and cyclophosphamide combined group 5 HBO exposures were given prophylactically before cyclophosphamide. The combination of mesna, HBO and cyclophosphamide was administered by the same procedure. The contractions obtained in response to acetylcholine (ACh, 10–4M) in the control group were reduced using cyclophosphamide and HBO individually, but not by mesna. However, the contractions belonging to the various combinations of these three agents were not different from those seen in the control group. On the other hand, the combinations of cyclophosphamide, mesna and HBO showed higher responses to ACh than the groups in which cyclophosphamide and HBO were used individually, while the responses elicited by the cyclophosphamide and HBO combination were greater than those seen in the group treated with HBO only.
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Nygaard, Rachel M., and Frederick W. Endorf. "517 Hyperbaric Oxygen and Mortality in Burns with Inhalation Injury: a Study of the National Burn Repository." Journal of Burn Care & Research 42, Supplement_1 (April 1, 2021): S105. http://dx.doi.org/10.1093/jbcr/irab032.168.
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Abstract Introduction Mortality in burn injury is primarily influenced by three factors: age, percent burn (%TBSA), and presence of inhalation injury. Numerous modalities have been tried in an attempt to treat those patients with burns and inhalation injury, including the use of hyperbaric oxygen (HBO). The aim of our study was to find the national prevalence of HBO for burns with inhalation injury, and whether HBO influenced mortality in these often severely injured patients. Methods This retrospective study used the National Burn Repository (NBR) to identify hospital admissions of patients with both cutaneous burns and inhalation injuries. After applying exclusion criteria, a total of 13,044 patients were identified. Variables included in the multivariate regression analysis included: age, sex, race, payer, mechanism of burn injury, TBSA group, total procedure number, mechanical ventilator days, and treatment with HBO. The main outcome variable was mortality. Results Of the 13,044 patients, 67 had HBO therapy. The HBO patients were older (mean age 51.7 years vs. 42.8 years, p< 0.001) but had smaller burns and thus a similar Baux score (66.6 vs. 65.2, p=0.661). The HBO patients had a higher mortality (29.9% vs. 17.5%, p= 0.01). On multivariate regression analysis, HBO was an independent predictor of mortality (OR= 2.484, p= 0.004). Other significant predictors of mortality included age, Black race, Medicaid or uninsured patients, and %TBSA. Conclusions The use of HBO for patients with burns and inhalation injury is uncommon in this database. It is unclear whether that reflects low prevalence or if individual centers do not all impute HBO into the NBR. For those patients in this database, HBO is an independent predictor of mortality. It can be difficult to determine the severity of inhalation injury in the NBR, so those patients receiving HBO could theoretically have more severe inhalation injury.
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Abdelhakim, Haitham, Sajjad Akbar Bhatti, Amy Rose Cantilena, Tara L. Lin, Siddhartha Ganguly, Anurag K. Singh, Sunil Abhyankar, et al. "Retrospective Analysis of Post-Transplant Complications in Autologous Hematopoietic Cell Transplantation Patients Receiving Hyperbaric Oxygen Therapy." Blood 128, no. 22 (December 2, 2016): 5816. http://dx.doi.org/10.1182/blood.v128.22.5816.5816.
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Abstract High-dose chemotherapy and autologous hematopoietic cell transplantation (Auto-HCT) is an established therapy for patients with multiple myeloma and lymphomas. Post-transplant complications during the neutropenia phase include pancytopenia requiring transfusions and G-CSF support, mucositis and infectious complications. Previously, we have demonstrated that hyperbaric oxygen therapy (HBO) given day 0 prior to transplant reduced erythropoietin (EPO) levels and improved engraftment in umbilical cord blood transplantation. Subsequently, we conducted a pilot trial to study HBO effects EPO levels and engraftment in Auto-HCT setting. Herein, we review HBO effects on Auto-HCT post-transplant complications. Methods: Charts of patient who underwent HBO treatment prior to Auto-HCT on our pilot study and matched historic controls were retrospectively reviewed for post-transplant transfusion requirements, G-CSF support, mucositis and infectious complications Results: Nineteen patients completed HBO therapy and were compared to 118 patients in our historic cohort. Basic patient characteristics are listed in table-1. Average days of G-CSF use was 6.2 days in our HBO cohort compared to 9.16 days in the historic cohort (P=0.0007). Average number of transfused packed red cells was 2.1 and 2.58 units (P=0.22), while average number of platelet unit transfusions was 2.5 and 3.2 (P=0.17) in the HBO and historic cohorts, respectively. Mucositis incidence was 26.3% in the HBO cohort compared to 59.3% in controls (P=0.008). Similarly, the rate of neutropenic fever was 47% in the HBO cohort vs. 65% in controls (P=0.11). The rate of admissions, on the other hand, was 31.5% in the HBO cohort compared to 23.7% in controls (P=0.31) with an average length of hospital stay of 6.6 days in the HBO cohort compared to 7.68 days in controls (P=0.31). Conclusions: HBO therapy prior to Auto-HCT is associated with lower G-CSF use and lower incidence of mucositis in our retrospective study. Prospective studies are needed to determine HBO effects on Auto-HCT post-transplant complications. Disclosures Ganguly: Onyx: Speakers Bureau; Seattle Genetics: Speakers Bureau; Janssen: Research Funding.
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Almzaiel, Anwar J., Richard Billington, Gary Smerdon, and A. John Moody. "Hyperbaric oxygen enhances neutrophil apoptosis and their clearance by monocyte-derived macrophages." Biochemistry and Cell Biology 93, no. 4 (August 2015): 405–16. http://dx.doi.org/10.1139/bcb-2014-0157.
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Neutrophil apoptosis and clearance by macrophages are essential for wound healing. Evidence suggests that hyperbaric oxygen (HBO) exposure may enhance neutrophil apoptosis, but HBO effects leading to neutrophil clearance by macrophages are still unclear. In the current study, bovine neutrophils and monocyte-derived macrophages (MDMΦ) were co-cultured under HBO (97.9% O2, 2.1% CO2 at 2.4 atm absolute (ATA)) (1 atm = 101.325 kPa), hyperbaric normoxia (8.8% O2 at 2.4 ATA), normobaric hyperoxia (95% O2, 5% CO2), normoxia (air), and normobaric hypoxia (5% O2, 5% CO2). Phagocytosis of fresh and 22 h aged neutrophils by MDMΦ was increased after HBO pre-treatment, assessed using flow cytometry and light microscopy. Enhanced clearance of neutrophils was accompanied by an increase in H2O2 levels following HBO pre-treatment with upregulation of IL-10 (anti-inflammatory cytokine) mRNA expression in LPS-stimulated MDMΦ that had ingested aged neutrophils. TNF-α (pro-inflammatory cytokine) gene expression did not change in LPS-stimulated MDMΦ that had ingested fresh or aged neutrophils after HBO, pressure, and hyperoxia. These findings suggest that HBO-activated MDMΦ participate in the clearance of apoptotic cells. Uptake of neutrophils by MDMΦ exposed to HBO may contribute to resolution of inflammation, because HBO induced up-regulation of IL-10 mRNA expression.
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da Rocha, Fernando, Djalma Fagundes, Jefferson Pires, and Fernanda Salim da Rocha. "Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats." Indian Journal of Plastic Surgery 45, no. 03 (September 2012): 453–58. http://dx.doi.org/10.4103/0970-0358.105941.
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ABSTRACT Objective: Our aim is to investigate the role of HBO (hyperbaric oxygen), NAC (N-acetylcysteine), and HBO plus NAC on the necrosis area of random rat′s skin flaps of a modified McFarlane flap design. Materials and Methods: Thirty-two male Wistar rats were randomly divided into four groups: G-S (sham: n = 8), G-NAC (NAC: n = 8), G-HBO (HBO: n = 8), and G-HN (HBO plus NAC: n = 8). A rectangular skin flap (2 × 8 cm 2 ) was dissected from the muscular dorsal layer, preserving the cranial pedicle. Polyethylene film was placed over the muscular layer and an interrupted 3.0 nylon suture was employed to fix the flap into the original place. On the eighth day, full-thickness biopsy samples (2 × 1 cm 2 ) were collected from the proximal, middle, and cranial areas of the skin flap, and in a site away from the flap labelled as the control area. Results: The measurements of necrotic areas in the groups were 18.3% in G-S, 24.3% in G-NAC, 12.6% in G-HBO, and 14.9% in G-HN. Significant difference was observed between the groups G-HBO and G-HN as well as G-NAC. Conclusion: HBO is associated with reduced area of necrosis of skin flap. The G-NAC group was associated with poor results when examined in isolation. The association between HBO and NAC did not produce favourable results with respect to the use of HBO alone. These findings suggest that the diffusion of oxygen through the interstitial space was the determining factor of more favourable results of HBO.
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Zanetti, Claude L. "History of HBO." Chest 95, no. 3 (March 1989): 706. http://dx.doi.org/10.1378/chest.95.3.706-b.
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Niefeldt, Alexander, and Andreas Kanstinger. "HBO für Anfänger." intensiv 24, no. 02 (March 7, 2016): 74–79. http://dx.doi.org/10.1055/s-0041-111125.
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Huang, Lixin, Maheshkumar P. Mehta, Anil Nanda, and John H. Zhang. "The role of multiple hyperbaric oxygenation in expanding therapeutic windows after acute spinal cord injury in rats." Journal of Neurosurgery: Spine 99, no. 2 (September 2003): 198–205. http://dx.doi.org/10.3171/spi.2003.99.2.0198.
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Object. Hyperbaric oxygenation (HBO) therapy has been reported to improve neurological recovery after spinal cord injury (SCI). In the present study, the authors examined whether multiple HBO therapy can expand the therapeutic window after acute SCI. Methods. Seventy rats were randomly assigned to seven groups: sham surgery; SCI without treatment; single HBO treatment beginning at 30 minutes, 3 hours, and 6 hours after SCI; and multiple HBO treatments starting at 6 and 24 hours postinjury. Mild SCI was induced by adjusting the height of a weight drop (10 g) to 6.25 mm above the exposed spinal cord. A single HBO administration was performed at 2.82 ata for 1 hour. The multiple HBO treatment modality was performed once daily for 1 week. All rats underwent behavioral testing with the Basso-Beattie-Breshnahan locomotor rating scale twice a week. Rats were killed on Day 42 postinjury and specimens comprising the lesioned area were histopathologically examined. Those rats that received single HBO intervention beginning at 30 minutes and 3 hours and those that received multiple HBO treatment starting at 6 hours following injury made significantly greater neurological recoveries than those in the nontreatment SCI group. These rats also retained more sparing tissue than controls. Conclusions. The results of this study demonstrate that multiple HBO treatments can expand the therapeutic window for acute SCI to 6 hours after injury.
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Yan, Wenjun, Zongping Fang, Qianzi Yang, Hailong Dong, Yan Lu, Chong Lei, and Lize Xiong. "Sirt1 Mediates Hyperbaric Oxygen Preconditioning-Induced Ischemic Tolerance in Rat Brain." Journal of Cerebral Blood Flow & Metabolism 33, no. 3 (January 9, 2013): 396–406. http://dx.doi.org/10.1038/jcbfm.2012.179.
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Our previous studies have shown that hyperbaric oxygen preconditioning (HBO-PC) induces tolerance to cerebral ischemia/reperfusion (I/R). This study aimed to investigate whether SirT1, a class III histone deacetylase, is involved in neuroprotection elicited by HBO-PC in animal and cell culture models of ischemia. Rats were subjected to middle cerebral artery occlusion for 120 minutes after HBO-PC (once a day for 5 days). Primary cultured cortical neurons were exposed to 2 hours of HBO-PC after 2 hours of oxygen–glucose deprivation (OGD). We showed that HBO-PC increased SirT1 protein and mRNA expression, promoted neurobehavioral score, reduced infarct volume, and improved morphology at 24 hours and 7 days after cerebral I/R. Neuroprotection of HBO-PC was attenuated by SirT1 inhibitor EX527 and SirT1 knockdown by short interfering RNA (siRNA), whereas it was mimicked by SirT1 activator resveratrol. Furthermore, HBO-PC enhanced SirT1 expression and cell viability and reduced lactate dehydrogenase release 24 hours after OGD/re-oxygenation. The neuroprotective effect of HBO-PC was emulated through upregulating SirT1 and, reversely, attenuated through downregulating SirT1. The modulation of SirT1 was made by adenovirus infection carrying SirT1 or SirT1 siRNA. Besides, SirT1 increased B-cell lymphoma 2 (Bcl-2) expression and decrease cleaved caspase 3. These results indicate that SirT1 mediates HBO-PC-induced tolerance to cerebral I/R through inhibition of apoptosis.
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Horie, Masaki, Mitsuhiro Enomoto, Manabu Shimoda, Atsushi Okawa, Shumpei Miyakawa, and Kazuyoshi Yagishita. "Enhancement of satellite cell differentiation and functional recovery in injured skeletal muscle by hyperbaric oxygen treatment." Journal of Applied Physiology 116, no. 2 (January 15, 2014): 149–55. http://dx.doi.org/10.1152/japplphysiol.00235.2013.
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Recently, the use of hyperbaric oxygen (HBO) treatments by elite athletes to accelerate recovery from muscle injuries has become increasingly popular. However, the mechanism of promoting muscle regeneration under HBO conditions has not yet been defined. In this study, we investigated whether HBO treatments promoted muscle regeneration and modulated muscle regulatory factor expression in a rat skeletal muscle injury model. Muscle injury was induced by injecting cardiotoxin (CTX) into the tibialis anterior (TA) muscles. As the HBO treatment, rats were placed in an animal chamber with 100% oxygen under 2.5 atmospheres absolute for 2 h/day, 5 days/wk for 2 wk. We then performed histological analyses, measured the maximum force-producing capacity of the regenerating muscle fibers, and performed quantitative RT-PCR analysis of muscle regulatory factor mRNAs. The cross-sectional areas and maximum force-producing capacity of the regenerating muscle fibers were increased by HBO treatment after injury. The mRNA expression of MyoD, myogenin, and IGF-1 increased significantly in the HBO group at 3 and 5 days after injury. The number of Pax7+/MyoD+, Pax7−/MyoD+, and Pax7+/BrdU+-positive nuclei was increased by HBO treatment. In this study, we demonstrated that HBO treatment accelerated satellite cell proliferation and myofiber maturation in rat muscle that was injured by a CTX injection. These results suggest that HBO treatment accelerates healing and functional recovery after muscle injury.
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Chin, Chun-Shih, Tsai-Yun Lee, Yi-Wen Chen, and Ming-Feng Wu. "Idiopathic Sudden Sensorineural Hearing Loss: Is Hyperbaric Oxygen Treatment the Sooner and Longer, the Better?" Journal of Personalized Medicine 12, no. 10 (October 5, 2022): 1652. http://dx.doi.org/10.3390/jpm12101652.
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(1) Background: We aimed to evaluate hearing benefits from hyperbaric oxygen (HBO) therapy in patients with Idiopathic Sudden Sensorineural Hearing Loss (ISSHL). (2) Methods: We performed a retrospective analysis of chart reviews on patients with ISSHL between Jan 2016 and Dec 2021. All patients were referred to receive HBO therapy by the department of Ear, Nose and Throat (ENT). Hearing gain was assessed based on pure-tone audiometry (PTA). Data were analyzed for 102 patients after 1 to 5 therapy sessions, and for 46 patients after 6 to 10 therapy sessions. (3) Results: After 1–5 HBO sessions, patients (N = 102) showed an improvement in 45 (44.1%) of the patients (p < 0.000). Also, improvements were found with patients showing different grades of ISSHL: 11 (26.8%) with slight-moderate, 11 (40.7%) with severe, and 23 (67.6%) with profound ISSHL. Significant treatment effects were found at different affected frequencies, especially the low frequency range. After 6–10 HBO sessions, patients (N = 46) showed similar treatment effects as after 1–5 HBO sessions, but no additional improvement. Moreover, patients who received HBO treatment within 12 days showed improvement effects 6.484 times greater (p < 0.000) compared with those who received treatment after 13 days. (4) Conclusions: The improvement of HBO therapy on ISSHL was significant after 1–5 sessions, with larger improvements for those suffering more serious symptoms. Further adding more HBO treatment sessions to 6–10, no further improvement was found. Patients starting HBO therapy within 12 days of ISSHL showed 6.484 times greater improvements compared with those starting HBO therapy later.
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Lin, Pao-Yuan, Pei-Hsun Sung, Sheng-Ying Chung, Shan-Ling Hsu, Wen-Jung Chung, Jiunn-Jye Sheu, Shu-Kai Hsueh, Kuan-Hung Chen, Re-Wen Wu, and Hon-Kan Yip. "Hyperbaric Oxygen Therapy Enhanced Circulating Levels of Endothelial Progenitor Cells and Angiogenesis Biomarkers, Blood Flow, in Ischemic Areas in Patients with Peripheral Arterial Occlusive Disease." Journal of Clinical Medicine 7, no. 12 (December 14, 2018): 548. http://dx.doi.org/10.3390/jcm7120548.
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Background: This study tested the hypothesis that hyperbaric oxygen (HBO) therapy enhanced the circulating levels of endothelial progenitor cells (EPCs), soluble angiogenesis factors, and blood flow in ischemic areas in patients with peripheral arterial occlusive disease (PAOD). Methods: In total, 57 consecutive patients with PAOD undergoing the HBO therapy (3 atmospheres (atm) for 2 h each time) were prospectively enrolled into the present study. Venous blood sampling was performed to assess the circulating levels of EPCs and soluble angiogenesis factors prior to and during five sessions of HBO therapy. Additionally, skin perfusion pressure (SPP), an indicator of blood flow in ischemic areas, was measured by moorVMS-PRES. Results: The results demonstrated that the circulating levels of EPCs (cluster of differentiation (CD)34+/CD133+/CD45dim, CD31+/CD133+/CD45dim, CD34+) and soluble angiogenesis factors—vascular endothelial growth factor/stromal cell-derived factor 1/hepatocyte growth factor/fibroblast growth factor (VEGF/SDF-1α/HGF/FGF) were significantly increased post-HBO therapy as compared to pre-HBO therapy (all p < 0.01). Additionally, Matrigel assay showed that the angiogenesis was significantly increased in post-HBO therapy as compared to prior to therapy (p < 0.001). Furthermore, SPP was significantly increased in the ischemic area (i.e., plantar foot and mean SPP of the ischemic foot) in post-HBO therapy as compared to pre-HBO therapy (all p < 0.01). Importantly, the HBO therapy did appear to result in complications, and all the patients were uneventfully discharged without amputation. Conclusions: HBO therapy augmented circulating levels of EPCs and angiogenesis factors, and improved the blood flow in the ischemic area.
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Smolle, Christian, Joerg Lindenmann, Lars Kamolz, and Freyja-Maria Smolle-Juettner. "The History and Development of Hyperbaric Oxygenation (HBO) in Thermal Burn Injury." Medicina 57, no. 1 (January 8, 2021): 49. http://dx.doi.org/10.3390/medicina57010049.
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Background and Objectives: Hyperbaric oxygenation (HBO) denotes breathing of 100% oxygen under elevated ambient pressure. Since the initiation of HBO for burns in 1965, abundant experimental and clinical work has been done. Despite many undisputedly positive and only a few controversial results on the efficacy of adjunctive HBO for burn injury, the method has not yet been established in clinical routine. Materials and Methods: We did a retrospective analysis of the literature according to PRISMA—guidelines, from the very beginning of HBO for burns up to present, trying to elucidate the question why HBO is still sidelined in the treatment of burn injury. Results: Forty-seven publications (32 animal experiments, four trials in human volunteers and 11 clinical studies) fulfilled the inclusion criteria. Except four investigators who found little or no beneficial action, all were able to demonstrate positive effects of HBO, most of them describing less edema, improved healing, less infection or bacterial growth and most recently, reduction of post-burn pain. Secondary enlargement of burn was prevented, as microvascular perfusion could be preserved, and cells were kept viable. The application of HBO, however, concerning pressure, duration, frequency and number of treatment sessions, varied considerably. Authors of large clinical studies underscored the intricate measures required when administering HBO in severe burns. Conclusions: HBO unquestionably has a positive impact on the pathophysiological mechanisms, and hence on the healing and course of burns. The few negative results are most likely due to peculiarities in the administration of HBO and possibly also to interactions when delivering the treatment to severely ill patients. Well-designed studies are needed to definitively assess its clinical value as an adjunctive treatment focusing on relevant outcome criteria such as wound healing time, complications, length of hospital stay, mortality and scar quality, while also defining optimal HBO dosage and timing.
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Smolle, Christian, Joerg Lindenmann, Lars Kamolz, and Freyja-Maria Smolle-Juettner. "The History and Development of Hyperbaric Oxygenation (HBO) in Thermal Burn Injury." Medicina 57, no. 1 (January 8, 2021): 49. http://dx.doi.org/10.3390/medicina57010049.
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Background and Objectives: Hyperbaric oxygenation (HBO) denotes breathing of 100% oxygen under elevated ambient pressure. Since the initiation of HBO for burns in 1965, abundant experimental and clinical work has been done. Despite many undisputedly positive and only a few controversial results on the efficacy of adjunctive HBO for burn injury, the method has not yet been established in clinical routine. Materials and Methods: We did a retrospective analysis of the literature according to PRISMA—guidelines, from the very beginning of HBO for burns up to present, trying to elucidate the question why HBO is still sidelined in the treatment of burn injury. Results: Forty-seven publications (32 animal experiments, four trials in human volunteers and 11 clinical studies) fulfilled the inclusion criteria. Except four investigators who found little or no beneficial action, all were able to demonstrate positive effects of HBO, most of them describing less edema, improved healing, less infection or bacterial growth and most recently, reduction of post-burn pain. Secondary enlargement of burn was prevented, as microvascular perfusion could be preserved, and cells were kept viable. The application of HBO, however, concerning pressure, duration, frequency and number of treatment sessions, varied considerably. Authors of large clinical studies underscored the intricate measures required when administering HBO in severe burns. Conclusions: HBO unquestionably has a positive impact on the pathophysiological mechanisms, and hence on the healing and course of burns. The few negative results are most likely due to peculiarities in the administration of HBO and possibly also to interactions when delivering the treatment to severely ill patients. Well-designed studies are needed to definitively assess its clinical value as an adjunctive treatment focusing on relevant outcome criteria such as wound healing time, complications, length of hospital stay, mortality and scar quality, while also defining optimal HBO dosage and timing.
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Wilson, Judy R., and Jeremiah Campbell. "Hyperbaric Oxygen Attenuates Aortic Vasoconstriction after Simulated Microgravity in Rats." Sports and Exercise Medicine – Open Journal 7, no. 1 (December 31, 2021): 6–11. http://dx.doi.org/10.17140/semoj-7-183.
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Background The hindlimb suspended (HLS) rat model has been used in land-based research to evaluate effects of simulated microgravity. Previous research demonstrated that 2-4 weeks of HLS reduced vasoconstrictive responses of aortic, mesenteric, and femoral arterial rings to phenylephrine (PHE) while acute exposure to hyperoxia amplified constrictive responses to PHE. The purpose of this study was to determine if hyperbaric oxygen treatment (HBO) during HLS would reverse the attenuation of the vasoconstrictive response. Methods Five-month-old male Sprague Dawley rats were randomly divided into aging controls (AC), AC-HBO, HLS, and HLS-HBO. Groups receiving HBO (AC-HBO; HLS-HBO) were placed in a cage that was fitted for the animal hyperbaric chamber to maintain HLS. HBO groups received 24 treatments, once a day, 6 d/week using a wound care protocol. The chamber was flushed with 100% oxygen, compressed over 10 min to 2.5 atmospheres absolute (ATA) (22.5 psig), a 90-minute treatment, then a 10 min decompression. After 28 d of HLS, animals were sacrificed under isoflurane anesthesia and thoracic aorta segments isolated. Relaxation of aortic rings was measured in response to acetylcholine (ACh) and sodium nitroprusside (SNP) after pre-constriction with PHE (3×10-7). Constriction of aortic rings was also determined in response to increasing concentrations of PHE. All drugs were administered cumulatively in vessel baths at 10-10-10-4 M. Data were analyzed using four-parameter (i.e., minimum, maximum, EC50, slope) nonlinear regression, and groups compared using 2×2 ANOVA with HBO and HLS as main effects. Results Responses to ACh and SNP were not affected by HLS or HBO. However, in response to PHE, there was a decrease in maximum vasoconstriction in HLS compared to controls (44.7±7.3% vs 82.4±6.0%, respectively, p≤0.05) and in HBO compared to controls (48.5±6.5% vs 78.6±6.8%, p≤0.05). Conclusion These results indicate that PHE-induced constriction of thoracic aorta is decreased after HLS. HBO did not reverse HLS-induced reductions in contractile responses; instead, HBO independently reduced PHE-stimulated constriction of aortic segments. This suggests that HBO may be useful in conditions where constriction is enhanced, such as diabetes.
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Biemans, Harm J. A., Hans Mariën, Erik Fleur, Tanya Beliaeva, and Jan Harbers. "Students’ experiences with different learning pathways to higher professional bachelor programmes." International Journal for Research in Vocational Education and Training 7, no. 1 (April 29, 2020): 1–20. http://dx.doi.org/10.13152/10.13152/ijrvet.7.1.1.
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Context: In the Dutch educational system, different learning pathways to higher professional bachelor (or HBO) programmes have been created: the regular VET route, the general secondary education route, and continuing learning pathways (such as the Green Lyceum or GL) that combine characteristics of these two traditional routes and that are specifically designed for students who combine a relatively high cognitive level with an affinity for practical, vocation-oriented assignments. Approach: The present study aimed to compare the experiences of students coming from these three different learning pathways to HBO. 62 former GL students, 127 former middle-management VET (or MBO) students, and 81 former regular general secondary education (or HAVO) students completed an online questionnaire on their experiences in their first HBO study year and their scores on the various scales were compared. Findings: Students from the three groups were equally satisfied with their current HBO programme. The same pattern was found for perceived study success in their HBO programme. Former GL students, however, mentioned that they had been better prepared in terms of development of study skills needed in HBO. Compared with the MBO route to HBO, former GL students felt better prepared in terms of the theoretical subjects addressed in their previous educational programme. In this regard, their level of theoretical preparation was comparable to that of former HAVO students. When the vocation-oriented aspects of education were concerned, however, the GL appeared to be comparable with the MBO route to HBO (in the HAVO curriculum, vocation-oriented preparation for HBO is absent). Moreover, former HAVO students scored lower than former GL and former MBO students with respect to the extent to which they felt that they had received support in choosing a particular HBO programme in their previous educational programme.Conclusion: The GL seems to combine the advantages of MBO and HAVO programmes for this particular group of students: theoretical preparation for HBO at HAVO level combined with practical preparation at MBO level, systematic career orientation and guidance, and development of study skills required in HBO. In other words, such continuing learning pathways can be a curriculum design solution for specific student groups to promote their transition to HBO.
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Biemans, Harm J. A., Hans Mariën, Erik Fleur, Tanya Beliaeva, and Jan Harbers. "Students’ experiences with different learning pathways to higher professional bachelor programmes." International Journal for Research in Vocational Education and Training 7, no. 1 (April 29, 2020): 1–20. http://dx.doi.org/10.13152/ijrvet.7.1.1.
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Context: In the Dutch educational system, different learning pathways to higher professional bachelor (or HBO) programmes have been created: the regular VET route, the general secondary education route, and continuing learning pathways (such as the Green Lyceum or GL) that combine characteristics of these two traditional routes and that are specifically designed for students who combine a relatively high cognitive level with an affinity for practical, vocation-oriented assignments. Approach: The present study aimed to compare the experiences of students coming from these three different learning pathways to HBO. 62 former GL students, 127 former middle-management VET (or MBO) students, and 81 former regular general secondary education (or HAVO) students completed an online questionnaire on their experiences in their first HBO study year and their scores on the various scales were compared. Findings: Students from the three groups were equally satisfied with their current HBO programme. The same pattern was found for perceived study success in their HBO programme. Former GL students, however, mentioned that they had been better prepared in terms of development of study skills needed in HBO. Compared with the MBO route to HBO, former GL students felt better prepared in terms of the theoretical subjects addressed in their previous educational programme. In this regard, their level of theoretical preparation was comparable to that of former HAVO students. When the vocation-oriented aspects of education were concerned, however, the GL appeared to be comparable with the MBO route to HBO (in the HAVO curriculum, vocation-oriented preparation for HBO is absent). Moreover, former HAVO students scored lower than former GL and former MBO students with respect to the extent to which they felt that they had received support in choosing a particular HBO programme in their previous educational programme.Conclusion: The GL seems to combine the advantages of MBO and HAVO programmes for this particular group of students: theoretical preparation for HBO at HAVO level combined with practical preparation at MBO level, systematic career orientation and guidance, and development of study skills required in HBO. In other words, such continuing learning pathways can be a curriculum design solution for specific student groups to promote their transition to HBO.
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Conconi, Maria Teresa, Silvia Baiguera, Diego Guidolin, Claudio Furlan, Anna M. Menti, Simonetta Vigolo, Anna S. Belloni, Pier Paolo Parnigotto, and Gastone G. Nussdorfer. "Effects of Hyperbaric Oxygen on Proliferative and Apoptotic Activities and Reactive Oxygen Species Generation in Mouse Fibroblast 3T3/J2 Cell Line." Journal of Investigative Medicine 51, no. 4 (July 2003): 227–32. http://dx.doi.org/10.1177/108155890305100424.
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Background Hyperbaric oxygen (HBO) therapy is widely used to treat problem wounds associated with pathologic conditions compromising blood supply and tissue oxygenation because increased tissue oxygen levels enhance collagen synthesis, cell proliferation, and angiogenesis. However, little is known about the dose of hyperoxia needed to achieve optimal therapeutic effects. Moreover, HBO, by enhancing the production of reactive oxygen species (ROS), may also exert cytotoxic effects. In vitro models are simplified systems that may aid the development of treatment protocols with HBO. Hence, we have investigated the effects of HBO on the growth and ROS production of the 3T3/J2 fibroblast cell line in relation to the pressure and the duration of exposure. Methods 3T3/J2 fibroblasts were plated (5 x 103 cells/cm2) on six-well microtiter plates in phosphate buffered saline (PBS), put in a compression chamber, and exposed to 100% oxygen at a pressure of 1.0 or 2.5 atmosphere absolute (ATA) for 15, 30, 60, or 120 minutes. Then the cells were incubated in Dulbecco's modified minimum essential medium (DMEM) for 24, 48, or 72 hours, and at the end of the post-HBO incubation period, their number was determined. In other experiments, cells were detached just after HBO exposure, seeded on 60 mm Petri dishes, and cultured for 10 days in DMEM, and the colony forming units were counted. The effects of HBO exposure (2.5 ATA) on the apoptotic rate of cultured cells were investigated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and enzyme-linked immunosorbent assays. To measure ROS production, 60 minutes before HBO exposure, 2',7'-dichlorofluorescin (DCF) diacetate (200 nmol/mL) was added to PBS, and after HBO exposure (2.5 ATA), cells were lysated, and fluorescence-emission intensity was measured and converted to μmol DCF/μg protein. Results At 1.0 ATA, all HBO exposures increased the proliferation rate of cultured fibroblasts and their clonal growth efficiency. At 2.5 ATA, 15-minute exposure to HBO was ineffective, whereas 30- and 60-minute exposures raised the proliferation rate and clonal growth efficiency. Conversely, a 120-minute exposure significantly decreased these parameters compared with control cultures. The exposure of cells to HBO at 2.5 ATA for 120 minutes raised the apoptotic rate of cultured fibroblasts, whereas shorter exposure times were ineffective. All exposure periods to HBO at 2.5 ATA enhanced ROS production from cultured fibroblasts. Conclusions Collectively, our findings allow us to conclude that (1) all of the exposure periods to HBO at 1.0 ATA or 30- and 60-minute periods at 2.5 ATA enhance cell growth, (2) 120-minute exposure to HBO at 2.5 ATA exerts a marked proapoptotic effect, and (3) no evident relationships occur between the effects of HBO on cell growth and ROS production.
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Cantilena, Amy Rose, Tara L. Lin, Siddhartha Ganguly, Leyla Shune, Anurag K. Singh, Sunil Abhyankar, Jonathan Mahnken, Dennis Allin, Joseph McGuirk, and Omar S. Aljitawi. "Impact of Hyperbaric Oxygen Treatment on Time to Transfusion Independency Post-UCB Transplant." Blood 126, no. 23 (December 3, 2015): 4333. http://dx.doi.org/10.1182/blood.v126.23.4333.4333.
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Abstract Background Limitations in umbilical cord blood (UCB) transplantations result from decreased cell numbers available for infusion at time of transplant. Delayed engraftment and higher rates of engraftment failure subsequently increase the need for post-transplant growth factor use and transfusion support. Hyperbaric oxygen (HBO) has been shown to improve engraftment in an animal model of UCB transplantation. These experiments proved sufficient to initiate a first-in-human trial of HBO for UCB transplantation. Objectives This study compared growth factor use and time to packed red blood cell (PRBC) and platelet transfusion independence between the HBO study population and historical UCB cases from the same institution. The effects of conditioning regimen and the number of cord units infused at time of transplant were analyzed. Study Design Subjects underwent HBO therapy at the University of Kansas Medical Center after reduced intensity conditioning (RIC) (n=9) or myeloablative conditioning (MAC) (n=6) regimens. Six hours following HBO therapy, they received single (n=8) or double (n=7) UCB units. Charts of HBO-treated patients and historical controls (n=44) were reviewed for post-transplant growth factor use and transfusion requirements. These were further stratified by preparative regimen and number of UCB units infused. Kaplan-Meier curves were compared between HBO and control subjects using log-rank tests as some observations were right-censored if the patient experienced relapse or expired within the first 100 days post-transplant. A small quantity was imputed to values of 0 to facilitate including these subjects in the analyses. Results By days +66 and +74 post-transplant, 100% of HBO-patients were PRBC and platelet independent, respectively. This compares to incomplete platelet (88.63%) and PRBC (86.36%) independence in the control cohort at day 100. Though time to transfusion independence (TTI) for PRBCs was consistently shorter in the HBO cohort, it was only statistically significant in the RIC setting and approached significance in the single cord setting (Table-1). TTI for platelets was shorter for the HBO cohort and approached statistical significance in the single cord setting. Similarly, the consecutive days of filgrastim support post-transplant were consistently fewer for HBO patients, with values approaching statistical significance in the MAC and single cord settings. Conclusions In vitro data with HBO and UCB showed improved rates of engraftment in animal models. Similarly, data in this small pilot study suggests that HBO facilitates less growth factor use and shorter TTI. However, these effects did not reach statistical significance in all settings, most likely due to small sample size of the HBO cohort. Further studies are needed to examine the effect of HBO on growth factor use, PRBC and platelet independence post-UCB transplantation. Table 1. A comparison of supportive transfusion means, between standard and HBO-UCB transplant recipients. These data are segregated for conditioning regimens and units of UCB infused for transplant. Group PRBC Units Platelet Units Days G-CSF Support TTI - PRBC TTI - Platelets HBO-total (n=15) 9 (p=0.30) 16.35 (p=0.31) 29.4 (p=0.08) 32.87 (p=0.07) 33.53 (p=0.11) Standard-Total (n=44) 9.29 17.14 35.02 56.09 54.8 HBO-Ablative (n=6) 5.43(p=0.23) 7.86 (p=0.16) 26.63 (p=0.07) 24 (p=0.53) 25.5 (p=0.45) Standard-Ablative (n=23) 11.93 22.29 35.65 66.13 67.78 HBO-RIC (n=9) 6.13 (p=0.66) 11.89 (p=0.78) 31.67 (p=0.44) 22.56 (p=0.02) 25.56 (p=0.11) Standard-RIC (n=21) 7.52 13.71 34.33 45.09 40.57 HBO-Single UCB (n=8) 5.43 (p=0.19) 7.87 (p=0.26) 26.63 (p=0.06) 24 (p=0.06) 25.5 (p=0.06) Standard-Single UCB (n=4) 10.25 24 36.2 74.4 70.2 HBO-Double UCB (n=7) 12.57 (p=0.87) 24.86 (p=0.95) 33.57 (p=0.70) 43 (p=0.74) 42.71 (p=0.95) Standard-Double UCB (n=40) 9.19 16.19 34.87 53.74 52.82 Disclosures No relevant conflicts of interest to declare.
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ZHANG, GUILAN, FEIBING XIONG, BAO ZHANG, GUOQING TANG, WENJU CHEN, LIANYING WANG, and YUBAI BAI. "EFFECT OF SOLVENT ON NONLINEAR REFRACTIVE INDEX OF 2-(2′-HYDROXYPHENYL) BENZOXAZOLE." Journal of Nonlinear Optical Physics & Materials 12, no. 03 (September 2003): 385–91. http://dx.doi.org/10.1142/s0218863503001511.
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Nonlinear refractive indexes n2 of 2-(2′-hydroxyphenyl) benzoxazole (HBO) in three species of solvent (cyclohexane, ethanol and dimethyl sulfoxide) have been determined by using the Z-scan technique. The experimental results show that the n2 of HBO is strongly dependent on the polarity of the solvent. Through the study on the absorption and fluorescence spectra of HBO in different solvents, we regard that the principal origin of the nonlinear refractive index of HBO is not the thermal effect because of absorption of incident light but the excited state intramolecular proton transfer of HBO under the incident light.
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Bertoletto, Paulo Roberto, José Carlos Chaves, Anna Tereza Negrini Fagundes, Ricardo Santos Simões, Celina Tizuko Fujiyama Oshima, Manuel de Jesus Simões, and Djalma José Fagundes. "Effect of different periods of hyperbaric oxygen on ischemia-reperfusion injury of rat small bowel." Acta Cirurgica Brasileira 23, no. 1 (February 2008): 11–15. http://dx.doi.org/10.1590/s0102-86502008000100003.
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PURPOSE: To determine whether hyperbaric oxygen (HBO) could effectively protect the small intestine mucosa against an ischemic insult, according to different periods of application. METHODS: The gut of 32 male rats was subjected to 60-min ischemia (clamping the mesenteric artery and vein); After they were further reperfused upon clamp opening during 60 min. Animal groups were as follows. GII = placed on HBO during the ischemia period; GIII = placed on HBO during reperfusion; GIV = treated with HBO throughout the ischemia-reperfusion period. Some animals (GI) did not receive HBO treatment at all and served as reference of ischemia-reperfusion injury (IR). HBO was carried out in a cylindrical acrylic chamber (2.0 ATA). Samples of small bowel were prepared for H.E staining for histological evaluations. RESULTS: The histological injury of mucosa was significantly less when HBO was administered during the ischemia period (17.6 ± 0.6) as compared with the IR (21.3 ± 1.8). HBO was not effective when applied during reperfusion (23.1 ± 2.1) or during the ischemia plus reperfusion period (18.7 ± 1.9). The thickness of the mucosa was preserved by HBO in ischemia (327.50 ± 30.23 µm) in comparison with the IR (172.79 ± 5.95 µm). In the periods of reperfusion (162.50 ± 6.05 µm) and ischemia plus reperfusion (296.49 ± 20.01 µm) the mucosa revealed a structural injury. CONCLUSION: Hyperbaric oxygen affects the ischemic insult of small bowel, being the favorable effect obtained when hyperbaric oxygen was administered early in the ischemic period.
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Hink, Jonas, Stephen R. Thom, Ulf Simonsen, Inger Rubin, and Erik Jansen. "Vascular reactivity and endothelial NOS activity in rat thoracic aorta during and after hyperbaric oxygen exposure." American Journal of Physiology-Heart and Circulatory Physiology 291, no. 4 (October 2006): H1988—H1998. http://dx.doi.org/10.1152/ajpheart.00145.2006.
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Accumulating evidence suggests that hyperbaric oxygen (HBO) stimulates neuronal nitric oxide (NO) synthase (NOS) activity, but the influence on endothelial NOS (eNOS) activity and vascular NO bioavailability remains unclear. We used a bioassay employing rat aortic rings to evaluate vascular NO bioavailability. HBO exposure to 2.8 atm absolute (ATA) in vitro decreased ACh relaxation. This effect remained unchanged, despite treatment with SOD-polyethylene glycol and catalase-polyethylene glycol, suggesting that the reduction in endothelium-derived NO bioavailability was independent of superoxide production. In vitro HBO induced contraction of resting aortic rings with and without endothelium, and these contractions were reduced by the NOS inhibitor Nω-nitro-l-arginine. In addition, in vitro HBO attenuated the vascular contraction produced by norepinephrine, and this effect was reversed by Nω-nitro-l-arginine, but not by endothelial denudation. These findings indicate stimulation of extraendothelial NO production during HBO exposure. A radiochemical assay was used to assess NOS activity in rat aortic endothelial cells. Catalytic activity of eNOS in cell homogenates was not decreased by HBO, and in vivo HBO exposure to 2.8 ATA was without effect on eNOS activity and/or vascular NO bioavailability in vitro. We conclude that HBO reduces endothelium-derived NO bioavailability independent of superoxide production, and this effect seems to be unrelated to a decrease in eNOS catalytic activity. In addition, HBO increases the resting tone of rat aortic rings and attenuates the contractile response to norepinephrine by endothelium-independent mechanisms that involve extraendothelial NO production.
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Eren, Esin, Furkan Yıldırım, Ozlem Giray, and Necat Yilmaz. "Hyperbaric Oxygen Treatment is Associated with Lipid Inflammatory Response Assessed Uding Serum Platelet Activating Factor." Polish Hyperbaric Research 67, no. 2 (June 1, 2019): 103–8. http://dx.doi.org/10.2478/phr-2019-0009.
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Abstract Hyperbaric oxygen (HBO) treatment is generally a relatively safe therapy for various conditions. However, there are some adverse side effects. For example HBO tratment has been reported to increase the production of free oxygen radicals(FRs). Furthermore, to our knowledge, no previous clinical research has been carried out to study the involvement of platelet-activating factor(PAF)as the lipid oxidative stressor in patients undergoing HBO treatment. A total of 45 patients included in this study were first given clinical assessment and laboratory measurements before starting HBO treatment and were named group baseline. After the HBO treatment, the same clinical and laboratory measurements from the same patients were repeated and this was named group sesion >20.As expected, long-term HBO treatment had no effect on oxLDL (oxidized low-density lipoprotein), a lipid oxidative stress(OS) marker. However, the mean PAF values in the second group showed a statistically significant increase compared to their pretreatment values, (P <0. 002).As this is a preliminary study, there is a need for more detailed investigations that demonstrate the association of HBO treatment with the lipid inflammatory response. Therefore, there is need for further clinical study for OS markers such as oxLDL in HBO treatment. Clinical prospective studies are required to confirm our laboratory findings.
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Dreval, A. V., V. A. Gubkina, S. O. Kiselev, R. S. Tishenin, G. S. Molchanov, and G. F. Cheskidov. "Induction and maintenance of remission of insulin-dependent diabetes mellitus in repeated courses of hyperbaric oxygenation." Problems of Endocrinology 45, no. 4 (August 15, 1999): 10–13. http://dx.doi.org/10.14341/probl11782.
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The efficacy of hyperbaric oxygenation (HBO) is studied in diabetics with newly detected or lasting for less than 1 year disease. The possibility of inducing and/or prolonging diabetes remission by HBO and the efficacy of repeated courses of HBO are evaluated. Fifty-three patients with insulin-dependent diabetes mellitus (IDDM) (28 men and 25 women) aged 15-39 years, suffering from the disease for up to 12 month without apparent complications, were administered intensive insulin therapy with human insulin preparations and 10-day HBO courses. HBO courses repeated every 4 months promoted compensation of carbohydrate metabolism by stimulating residual insulin secretion in patients with IDDM lasting for up to one year, the effect of HBO progressively decreasing with each course. Manifest positive effect of HBO persisted for 2 months. After the first course of HBO, remission of IDDM ensued in 41.5% cases. Patients aged over 25 years with intact insulin secretion on an empty stomach were more disposed to remission. Remissions were equally incident in patients with diabetes duration of up to 6 months and in those with diabetes duration of 6-12 months. A history of ketoacidotic coma episodes does not rule out the induction of a remission.
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Wang, Yong-Gang, Jiang Long, Dong-Chuan Shao, and Hai Song. "Hyperbaric oxygen inhibits production of CD3+ T cells in the thymus and facilitates malignant glioma cell growth." Journal of International Medical Research 46, no. 7 (May 22, 2018): 2780–91. http://dx.doi.org/10.1177/0300060518767796.
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Objective Hyperbaric oxygen (HBO) is an emerging complementary alternative medical approach in glioma treatment. However, its mode of action is unknown, so this was investigated in the present study. Methods We constructed an intracranial glioma model of congenic C57BL/6J mice. Glioma growth under HBO stimulation was assessed by bioluminescent imaging and magnetic resonance imaging. Flow cytometry assessed direct effects of HBO on reactive oxygen species (ROS) signaling of transplanted glioma cells and organs, and quantified mature T cells and subgroups in tumors, the brain, and blood. Results HBO promoted the growth of transplanted GL261-Luc glioma in the intracranial glioma mouse model. ROS signaling of glioma cells and brain cells was significantly downregulated under HBO stimulation, but thymus ROS levels were significantly upregulated. CD3+ T cells were significantly downregulated, while both Ti/Th cells (CD3+CD4+) and Ts/Tc cells (CD3+CD8+) were inhibited in tumors of the HBO group. The percentage of regulatory T cells in Ti/Th (CD3+CD4+) cells was elevated in the tumors and thymuses of the HBO group. Conclusion HBO induced ROS signaling in the thymus, inhibited CD3+ T cell generation, and facilitated malignant glioma cell growth in vivo in the intracranial glioma mouse model.
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Qin, Li, Chu Meihua, Guo Dadong, Wang Li, Wang Jinglin, Ding Xiaoyu, Bi Mingjun, and Zou Yong. "Efficacy of Combined XingZhi-YiNao Granules and Hyperbaric Oxygen Therapy for Cognition and Motor Dysfunction in Patients with Delayed Encephalopathy after Acute Carbon Monoxide Poisoning." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/1323297.
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Purpose. To investigate the efficacy of XingZhi-YiNao (XZYN) granules and hyperbaric oxygenation (HBO) for cognition and motor dysfunction in patients with delayed encephalopathy after acute carbon monoxide poisoning (DEACMP). Methods. Eighty-nine patients with DEACMP were randomly divided into control group (n=19), HBO group (n=32), and XZYN group (n=38). All patients received conventional treatment. HBO group received HBO therapy once daily. XZYN group received extra XZYN granules plus HBO treatment. The related indexes including activity of daily living (ADL) scale, Montreal cognitive assessment (MoCA) scale, and mini mental state examination (MMSE) scale were measured. Cerebral white matter injury, age related white matter changes (ARWMC) scale, and the amplitude and latency of P300 were assessed. Results. Compared with control group, the neurological function scores of ADL, MoCA, and MMSE in HBO and XZYN groups were significantly improved, the impairment degree of brain white matter and cognition function were obviously alleviated, the latencies of P300 were significantly shortened, and the amplitudes of P300 were evidently increased (P<0.05). Treatment efficacy of XZYN group was superior to that of HBO group (P<0.05). Conclusion. Combined XZYN granules and HBO can significantly improve cognition and motor functions in patients with DEACMP.
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Nguyen-Ba, Truong, Abdulrahman Alzahrani, Thierry Giordano, and Remi Vaillancourt. "On contractivity-preserving 2- and 3-step predictor-corrector series for ODEs." Journal of Modern Methods in Numerical Mathematics 8, no. 1-2 (January 4, 2017): 17. http://dx.doi.org/10.20454/jmmnm.2017.1130.
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New optimal, contractivity-preserving (CP), \(d\)-derivative, 2- and 3-step, predictor-corrector, Hermite-Birkhoff-Obrechkoff series methods, denoted by \(HBO(d,k,p)\), \(k=2,3\), with nonnegative coefficients are constructed for solving nonstiff first-order initial value problems \(y'=f(t,y)\), \(y(t_0)=y_0\). The upper bounds \(p_u\) of order \(p\) of \(HBO(d,k,p)\), \(k=2,3\) methods are approximately 1.4 and 1.6 times the number of derivatives \(d\), respectively. Their stability regions have generally a good shape and grow with decreasing \(p-d\). Two selected CP HBO methods: 9-derivative 2-step HBO of order 13, denoted by HBO(9,2,13), which has maximum order 13 based on the CP conditions, and 8-derivative 3-step HBO of order 14, denoted by HBO(8,3,14), compare well with Adams-Cowell of order 13 in PECE mode, denoted by AC(13), in solving standard N-body problems over an interval of 1000 periods on the basis of the relative error of energy as a function of the CPU time. They also compare well with AC(13) in solving standard N-body problems on the basis of the growth of relative error of energy and 10000 periods of integration. The coefficients of selected HBO methods are listed in the appendix.
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Gregorevic, Paul, Gordon S. Lynch, and David A. Williams. "Hyperbaric oxygen improves contractile function of regenerating rat skeletal muscle after myotoxic injury." Journal of Applied Physiology 89, no. 4 (October 1, 2000): 1477–82. http://dx.doi.org/10.1152/jappl.2000.89.4.1477.
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There is growing interest in hyperbaric oxygen (HBO) as an adjunctive treatment for muscle injuries. This experiment tested the hypothesis that periodic inhalation of HBO hastens the functional recovery and myofiber regeneration of skeletal muscle after myotoxic injury. Injection of the rat extensor digitorum longus (EDL) muscle with bupivacaine hydrochloride causes muscle degeneration. After injection, rats breathed air with or without periodic HBO [100% O2 at either 2 or 3 atmospheres absolute (ATA)]. In vitro maximum isometric tetanic force of injured EDL muscles and regenerating myofiber size were unchanged between 2 ATA HBO-treated and untreated rats at 14 days postinjury but were ∼11 and ∼19% greater, respectively, in HBO-treated rats at 25 days postinjury. Maximum isometric tetanic force of injured muscles was ∼27% greater, and regenerating myofibers were ∼41% larger, in 3 ATA HBO-treated rats compared with untreated rats at 14 days postinjury. These findings demonstrate that periodic HBO inhalation increases maximum force-producing capacity and enhances myofiber growth in regenerating skeletal muscle after myotoxic injury with greater effect at 3 than at 2 ATA.
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Xiong-Ming, Du, Pan Zhao-E, Sun Jun-Ling, Zhou Zhong-Li, and Pang Bao-Yin. "Characterization of trait variations in progenies of Gossypium hirsutum transformed with the genomic DNA of Gossypium barbadense." Chinese Journal of Agricultural Biotechnology 2, no. 1 (April 2005): 39–44. http://dx.doi.org/10.1079/cjb200550.
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AbstractFour variant lines, HB1, HB2, HB3 and HB4, were derived from progenies of upland cotton (Gossypium hirsutum) cultivar Yumian 17 that had been transformed with genomic DNA from sea island cotton, Gossypium barbadense, using the pollen tube pathway (PTP) method. They showed significant differences from the wild-type recipient in terms of fibre quality, lint percentage, boll weight and other agronomic traits. The four variant lines also differed from the wild-type recipient in the amplification products of two SSR loci, indicating that the DNA of G. barbadense had been introduced into the genome of the recipient and was inherited stably. On the other hand, the agronomic traits of another three lines (HB5, HB6 and HB7) derived from the same transformation experiment were identical to those of the wild-type recipient, indicating that G. barbadense DNA had not integrated into the genome of these lines. A combination of the analyses on agronomic traits and SSR markers indicated that transgenic upland cotton strains produced by the PTP method could become homozygous in only a few generations and the foreign DNA introduced during the transformation experiments could be inherited stably in the transgenic progenies.
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Dong, Hailong, Lize Xiong, Zhenghua Zhu, Shaoyang Chen, Lichao Hou, and Takefumi Sakabe. "Preconditioning with Hyperbaric Oxygen and Hyperoxia Induces Tolerance against Spinal Cord Ischemia in Rabbits." Anesthesiology 96, no. 4 (April 1, 2002): 907–12. http://dx.doi.org/10.1097/00000542-200204000-00018.
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Background The aim of this study was to determine if the ischemic tolerance could be induced in the spinal cord by pretreatment with hyperbaric oxygen (HBO) and what components of HBO (hyperoxia, hyperbaricity, and combination of these two) were critical in the induction of tolerance against ischemic injury. Methods In experiment 1, 21 rabbits were randomly assigned to one of three groups (n = 7 each): animals in the control group received no HBO before spinal cord ischemia; animals in the HBO-1 and HBO-2 groups received HBO (2.5 atmosphere absolute [ATA], 100% O2) pretreatment 1 h/day for 3 and 5 days before ischemia, respectively. In experiment 2, 48 rabbits were randomly assigned to one of four groups (n = 12 each): the control group received no HBO (21% O2, 1 ATA, 1 h/day, 5 days) before spinal cord ischemia; the HB group received 1-h treatment in 21% O2 at 2.5 ATA each day for 5 days; the HO group received 1-h treatment in 100% oxygen at 1 ATA each day for 5 days; and the HBO group received HBO (2.5 ATA, 100% O2) treatment 1 h/day for 5 days. Twenty-four hours after the last treatment, spinal cord ischemia was induced by an infrarenal aorta clamping for 20 min. Forty-eight hours after reperfusion, hind-limb motor function and histopathology of the spinal cord were examined in a blinded fashion. Results In experiment 1, the neurologic outcome in the HBO-2 group was better than that of the control group (P = 0.004). The number of normal neurons in the anterior spinal cord in the HBO-2 group was more than that of the control group (P = 0.021). In experiment 2, the neurologic and histopathologic outcomes in the HBO group were better than that of the control group (P < 0.01). The histopathologic outcome in the HO group was better than that in the control group (P < 0.05). Conclusions Serial exposure to high oxygen tension induced ischemic tolerance in spinal cord of rabbits. Simple hyperbaricity (2.5 ATA, 21% O2) did not induce ischemic tolerance.
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Widiyanti, Prihartini, and Purnomo Suryohudoyo. "The role of hyperbaric oxygen to platelet aggregation in noninsulin-dependent diabetes mellitus (NIDDM)." Journal of Basic and Clinical Physiology and Pharmacology 32, no. 4 (June 25, 2021): 617–21. http://dx.doi.org/10.1515/jbcpp-2020-0481.
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Abstract Objectives Hyperglycemia in diabetes mellitus (DM) could cause rheological disorder, such as platelet aggregation and blood hyperviscosity. Hyperbaric oxygen (HBO) could decrease collagen as platelet aggregation agonist. This study aimed to explore the effect of HBO treatment to platelet aggregation parameters (latency time(LT), aggregation speed, aggregation index, and aggregation percentage) with the collagen aggregator in the noninsulin dependent diabetes mellitus (NIDDM). Methods The number of subjects in this study were 16 for each group normoxia normobaric (NONB) and HBO. NIDDM patients from DM polyclinic in Rumah Sakit Angkatan Laut (RSAL) Dr Ramelan Surabaya which was fulfilled inclusion criteria would receive HBO Therapy. Control Group/NONB were treated with NONB condition (20% O2 1 ATA) for 90 min and treatment group/HBO were treated with hyperoxia hyperbaric condition (100% O2 2.4 ATA) for 3 × 30 min with interval of 2 × 5 min for inhaling fresh air. Subject has been blood taken for platelet aggregation test before and after HBO Therapy. The length of treatment was 5 days for both condition (NONB and HBO). Results The data from both groups, NONB and HBO were tested first by normality test, homogenity test, correlation test, analysis of covariance, and paired t-test. Based on paired t-test, the decrease on platelet aggregation speed, aggregation index, and aggregation percentage after HBO treatment was showed significant difference on the LT and aggregation index while in aggregation speed and aggregation percentage was not significant. NONB group after 5 days was showed a significant difference on the aggregation speed and aggregation index while in LT and aggregation percentage was not significant. Conclusions The utilization of HBO 2.4 ATA 100% O2 3 × 30 min, once a day, for 5 days could decrease the platelet aggregation parameters (LT, aggregation speed, aggregation index, and aggregation percentage) in patients with NIDDM.
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Amin, Hesham M., Marco Cigada, Tawfic S. Hakim, and Enrico M. Camporesi. "Pulmonary mechanical and vascular responses after acute hyperbaric oxygen exposure." Canadian Journal of Physiology and Pharmacology 71, no. 8 (August 1, 1993): 592–96. http://dx.doi.org/10.1139/y93-083.
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Pulmonary mechanical and vascular responses were studied in Sprague–Dawley rats exposed to hyperbaric oxygen (HBO, 100% O2 at 2.8 atm (1 atm = 101.325 kPa) for 6 h). Two groups of animals were examined after HBO exposure: group HBO comprised rats examined immediately after exposure and group R consisted of rats left recovering while breathing air for 24 h before being studied. Both groups were compared with control rats, group C, not exposed to HBO. Pulmonary mechanical responses were measured by quantitating static lung compliance (Cs), wet to dry weight ratio (W/D) of lung tissue, and surface tension (ST) of broncho-alveolar lavage as a measurement of lung surfactant. Cs and W/D did not change significantly after exposure to HBO. ST was slightly lower in group R compared with group C (18.46 ± 1.46 vs. 22.47 ± 0.91 dynes/cm (1 dyne = 10 μN); p < 0.05). The effects of HBO on the pulmonary vasculature were studied in an isolated perfused lung preparation. Pulmonary vascular responses to hypoxia were recorded repeatedly over a period of 1 h. The percent increase in pulmonary vascular resistance (PVR) with hypoxia was blunted in group HBO and returned to the normal level in group R (8.2 ± 2.4% in group HBO vs. 44.2 ± 7.2% in group C and 42.9 ± 11.2% in group R; p < 0.001). In a subgroup of rats, to assess the vascular smooth muscle contractility, 0.1 mL of KCl (15 mmol/L) was added to the perfusate and the pulmonary vascular responses were also recorded. The vascular response to KCl was less in group R (86.6 ± 10.1% in R vs. 162.6 ± 12.2% in C and 179.0 ± 23.2% in HBO; p < 0.01). Our study shows that acute HBO exposure induces only minor pulmonary mechanical changes, while pulmonary vascular responses are markedly altered.Key words: hyperbaric oxygen, oxygen toxicity, lung mechanics, surfactant, pulmonary vasculature.