Academic literature on the topic 'HC-Pro'

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Journal articles on the topic "HC-Pro"

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Stenger, Drake C., Brock A. Young, Feng Qu, T. Jack Morris, and Roy French. "Wheat streak mosaic virus Lacking Helper Component-Proteinase Is Competent to Produce Disease Synergism in Double Infections with Maize chlorotic mottle virus." Phytopathology® 97, no. 10 (2007): 1213–21. http://dx.doi.org/10.1094/phyto-97-10-1213.

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The tritimovirus Wheat streak mosaic virus (WSMV) and the machlomovirus Maize chlorotic mottle virus (MCMV) each cause systemic chlorosis in infected maize plants. Infection of maize with both viruses produces corn lethal necrosis disease (CLND). Here, we report that complete deletion of the WSMV helper component-proteinase (HC-Pro) coding region had no effect on induction of CLND symptoms following coinoculation of maize with WSMV and MCMV. We further demonstrated that elevation of virus titers in double infections, relative to single infections, also was independent of WSMV HC-Pro. Thus, unl
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Jamous, Rana M., Kajohn Boonrod, Marc W. Fuellgrabe, Mohammed S. Ali-Shtayeh, Gabi Krczal, and Michael Wassenegger. "The helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Hua Enhancer 1 methyltransferase activity in vitro." Journal of General Virology 92, no. 9 (2011): 2222–26. http://dx.doi.org/10.1099/vir.0.031534-0.

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The helper component-proteinase (HC-Pro) is a multifunctional protein found among potyviruses. With respect to its silencing suppressor function, small RNA binding appears to be the major activity of HC-Pro. HC-Pro could also exhibit other suppressor activities. HC-Pro may inhibit the Hua Enhancer 1 (HEN1) activity. There is indirect evidence showing that either transient or stable expression of HC-Pro in plants results in an increase of non-methylated small RNAs. Here, we demonstrated that recombinant Zucchini yellow mosaic virus (ZYMV) HC-Pro inhibited the methyltransferase activity of HEN1
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Maia, I. G., A. L. Haenni, and F. Bernardi. "Potyviral HC-Pro: a multifunctional protein." Journal of General Virology 77, no. 7 (1996): 1335–41. http://dx.doi.org/10.1099/0022-1317-77-7-1335.

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Lim, Hyoun Sub, Tae Seok Ko, Houston A. Hobbs, et al. "Soybean mosaic virus Helper Component-Protease Alters Leaf Morphology and Reduces Seed Production in Transgenic Soybean Plants." Phytopathology® 97, no. 3 (2007): 366–72. http://dx.doi.org/10.1094/phyto-97-3-0366.

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Transgenic soybean (Glycine max) plants expressing Soybean mosaic virus (SMV) helper component-protease (HC-Pro) showed altered vegetative and reproductive phenotypes and responses to SMV infection. When inoculated with SMV, transgenic plants expressing the lowest level of HC-Pro mRNA and those transformed with the vector alone initially showed mild SMV symptoms. Plants that accumulated the highest level of SMV HC-Pro mRNA showed very severe SMV symptoms initially, but after 2 weeks symptoms disappeared, and SMV titers were greatly reduced. Analysis of SMV RNA abundance over time with region-s
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Boonrod, Kajohn, Marc W. Füllgrabe, Gabi Krczal, and Michael Wassenegger. "Analysis of the autoproteolytic activity of the recombinant helper component proteinase from zucchini yellow mosaic virus." Biological Chemistry 392, no. 10 (2011): 937–45. http://dx.doi.org/10.1515/bc.2011.097.

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AbstractThe multifunctional helper component proteinase (HC-Pro) of potyviruses contains an autoproteolytic function that, together with the protein 1 (P1) and NIa proteinase, processes the polyprotein into mature proteins. In this study, we analysed the autoproteolytic active domain of zucchini yellow mosaic virus (ZYMV) HC-Pro. SeveralEscherichia coli-expressed MBP:HC-Pro:GFP mutants containing deletions or point mutations at either the N- or C-terminus of the HC-Pro protein were examined. Our results showed that amino acids essential for the proteolytic activity of ZYMV HC-Pro are distinct
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Stenger, Drake C., Roy French, and Frederick E. Gildow. "Complete Deletion of Wheat Streak Mosaic Virus HC-Pro: a Null Mutant Is Viable for Systemic Infection." Journal of Virology 79, no. 18 (2005): 12077–80. http://dx.doi.org/10.1128/jvi.79.18.12077-12080.2005.

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ABSTRACT A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate that WSMV HC-Pro is dispensable for replication and movement, two essential processes that ar
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Dombrovsky, Aviv, Natan Gollop, Songbi Chen, Nor Chejanovsky, and Benjamin Raccah. "In vitro association between the helper component–proteinase of zucchini yellow mosaic virus and cuticle proteins of Myzus persicae." Journal of General Virology 88, no. 5 (2007): 1602–10. http://dx.doi.org/10.1099/vir.0.82769-0.

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Potyviruses, as typical non-persistently transmitted viruses, are carried within the stylets of aphids. Cuticle proteins (CuPs), which are a major component of the insect cuticle, were examined for in vitro binding to the potyviral helper component–proteinase (HC–Pro). Proteins in 8 M urea extracts from Myzus persicae were separated by SDS-PAGE, electroblotted onto membranes and identified as CuPs by using specific antibodies to M. persicae CuP. Blotted M. persicae protein extracts were overlaid with two HC–Pros, differing by the presence of K or E in the KLSC domain. The HC–Pro with KLSC, kno
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Goytia, Elisa, Lourdes Fernández-Calvino, Belén Martínez-García, Dionisio López-Abella, and Juan José López-Moya. "Production of plum pox virus HC-Pro functionally active for aphid transmission in a transient-expression system." Journal of General Virology 87, no. 11 (2006): 3413–23. http://dx.doi.org/10.1099/vir.0.82301-0.

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Potyviruses are non-persistently transmitted by aphid vectors with the assistance of a viral accessory factor known as helper component (HC-Pro), a multifunctional protein that is also involved in many other essential processes during the virus infection cycle. A transient Agrobacterium-mediated expression system was used to produce Plum pox virus (PPV) HC-Pro in Nicotiana benthamiana leaves from constructs that incorporated the 5′ region of the genome, yielding high levels of HC-Pro in agroinfiltrated leaves. The expressed PPV HC-Pro was able to assist aphid transmission of purified virus par
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Wu, Hui-Wen, Shih-Shun Lin, Kuan-Chun Chen, Shyi-Dong Yeh, and Nam-Hai Chua. "Discriminating Mutations of HC-Pro of Zucchini yellow mosaic virus with Differential Effects on Small RNA Pathways Involved in Viral Pathogenicity and Symptom Development." Molecular Plant-Microbe Interactions® 23, no. 1 (2010): 17–28. http://dx.doi.org/10.1094/mpmi-23-1-0017.

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Helper component-proteinase (HC-Pro), the gene-silencing suppressor of Potyvirus spp., interferes with microRNA (miRNA) and short-interfering RNA (siRNA) pathways. Our previous studies showed that three mutations of highly conserved amino acids of HC-Pro, R180I (mutation A), F205L (B), and E396N (C), of Zucchini yellow mosaic virus (ZYMV) affect symptom severity and viral pathogenicity. The mutant ZYMV GAC (ZGAC) with double mutations, R180I/E396N, induces transient leaf mottling in host plants followed by recovery. This mutant confers complete cross protection against subsequent infection by
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Jin, Yongsheng, Dongyuan Ma, Jiangli Dong, et al. "HC-Pro Protein of Potato Virus Y Can Interact with Three Arabidopsis 20S Proteasome Subunits In Planta." Journal of Virology 81, no. 23 (2007): 12881–88. http://dx.doi.org/10.1128/jvi.00913-07.

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ABSTRACT The multifunctional protein helper component proteinase (HC-Pro) is thought to interfere with the activity of the 20S proteasome; however, no sites of interaction have been identified for either protein. Here, we first show that the Potato virus Y (PVY) HC-Pro protein can interact with three Arabidopsis 20S proteasome subunits (PAA, PBB, and PBE), using a yeast two-hybrid system and the bimolecular fluorescence complement assay. In addition, yeast two-hybrid analysis of the interaction between several mutant subunits of the 20S proteasome and PVY HC-Pro confirmed that residues 81 to 1
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Dissertations / Theses on the topic "HC-Pro"

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Frangioni, Desiré Spada dos Santos [UNESP]. "Análise molecular da proteína HC-Pro (Helper Component-Proteinase) e seu papel na relação patógeno-hospedeiro." Universidade Estadual Paulista (UNESP), 2006. http://hdl.handle.net/11449/105388.

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Made available in DSpace on 2014-06-11T19:34:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-11-29Bitstream added on 2014-06-13T19:24:02Z : No. of bitstreams: 1 frangioni_dss_dr_botfca.pdf: 2302780 bytes, checksum: a3bab8cdfaa8a760d70900c13de09d8d (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>O gênero Potyvirus é um dos maiores dentre os vírus de planta com genoma composto por RNA. O genoma dos potyvirus codifica
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Frangioni, Desiré Spada dos Santos 1968. "Análise molecular da proteína HC-Pro (Helper Component-Proteinase) e seu papel na relação patógeno-hospedeiro /." Botucatu : [s.n.], 2006. http://hdl.handle.net/11449/105388.

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Resumo: O gênero Potyvirus é um dos maiores dentre os vírus de planta com genoma composto por RNA. O genoma dos potyvirus codifica um único polipeptídeo que é processado por três proteinases virais que originam todas as proteínas necessárias para complementar o ciclo da infecção. A proteína HC-Pro (Helper Component proteinase) é uma dessas proteínas multifuncionais que está envolvida na amplificação do genoma, transmissão por afídeo, movimento sistêmico e local, supressão do silenciamento gênico e proteólise. Nesse trabalho, foi realizada a substituição funcional de parte da região codificador
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Pereira, Tiago Campos. "Estudo da atividade de supressão da proteina HC-Pro e analise do perfil de expressão de PTGS em cana-de-açucar." [s.n.], 2002. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317226.

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Orientador : Paulo Arruda<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-08-03T12:02:30Z (GMT). No. of bitstreams: 1 Pereira_TiagoCampos_M.pdf: 5014451 bytes, checksum: d2047dfc11426a4b6bf79cbe548a81ec (MD5) Previous issue date: 2002<br>Mestrado
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URCUQUI, INCHIMA SILVIO. "Caracterisation des domaines de la proteine facteur assistant-proteinase (hc-pro) des potyvirus impliques dans l'interaction avec l'arn et dans l'homodimerisation." Paris 6, 1999. http://www.theses.fr/1999PA066508.

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Le genome des potyvirus (famille potyviridae), est constitue d'un arn simple brin de polarite positive. Il code pour une polyproteine qui subit une maturation proteolytique grace a trois proteinases virales, p1, la proteine facteur assistant-proteinase (hc-pro) et nia. Les potyvirus sont transmis par des pucerons, processus qui implique la capside virale et hc-pro. La proteine hc-pro est aussi impliquee dans la replication du genome viral. Il a recemment ete montre que cette proteine presente une activite de fixation a l'arn. Il a egalement ete suggere que la forme active de hc-pro correspond
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De, Godoy Maia Ivan. "Aspects moleculaires de la transmission du virus y de la pomme de terre (pvy) : caracterisation de la proteine facteur assistant (hc-pro)." Paris 11, 1996. http://www.theses.fr/1996PA112021.

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Les potyvirus sont transmis par des pucerons selon le mode non persistant ; deux proteines d'origine virale sont directement impliquees dans ce processus, la proteine de capside (cp) et la proteine facteur assistant de la transmission (hc-pro). Afin de mettre en evidence une activite eventuelle de fixation de la proteine hc-pro aux acides nucleiques, le gene codant pour l'hc-pro du virus y de la pomme de terre (pvy) a ete clone et sequence. Cette proteine a ensuite ete exprimee dans escherichia coli sous forme d'une proteine de fusion avec la proteine de fixation au maltose et purifiee par chr
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Jamoos, Rana [Verfasser], and Goetz [Akademischer Betreuer] Reustle. "Analysis of the interaction between the helper component proteinase (HC-Pro) of Zucchini yellow mosaic virus (ZYMV) and the plant RNA methyltransferase Hua enhancer 1 (HEN1) / Rana Jamoos. Betreuer: Goetz Reustle." Hohenheim : Kommunikations-, Informations- und Medienzentrum der Universität Hohenheim, 2012. http://d-nb.info/1027354203/34.

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Alexová, Martina. "Význam sportu pro propagaci města v cestovním ruchu na příkladu ledního hokeje." Master's thesis, Vysoká škola ekonomická v Praze, 2011. http://www.nusl.cz/ntk/nusl-116529.

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Main goal of this thesis is to find out, whether the fact, that in Znojmo used to be played the highest ice-hockey league in the Czech republic, led to the economic development of this region and to the development of tourism in this region. The theory part is focused on the theories which refer to this problem, particularly the theories of tourism and theories of economy of sport. The practice part at first analyses the Znojmo region and the ice-hockey club in Znojmo. The next chapter compares the budgets of the ice-hockey club in the different leagues, which the club used to join. The next c
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Kalebe-Nyamongo, Chipiliro Florence. "Elite attitudes towards the poor and pro-poor policy in Malawi." Thesis, University of Birmingham, 2012. http://etheses.bham.ac.uk//id/eprint/3398/.

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This thesis uses the theory of ‘social consciousness’ to analyse elite perceptions of poverty in Malawi,and identify the circumstances under which elites are willing to mobilize resources for poverty reduction. ‘Social Consciousness theory’ stipulates that pro-poor policies in European welfare states developed as a result of ideological and pragmatic concern about the negative impact of poverty on elite welfare. This study shows however that although elites in Malawi have a deep understanding and appreciation of the extent and severity of poverty and are linked with the poor through strong soc
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Bureš, Michal. "Návrh komunikační kampaně pro slavný český hokejový klub." Master's thesis, Vysoké učení technické v Brně. Fakulta podnikatelská, 2020. http://www.nusl.cz/ntk/nusl-416902.

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This master thesis analyzes the sport marketing practices of a successful ice-hockey club in the Czech Republic. After the theoretical definition of the terms, the thesis discusses the surrounding environment of the selected club and evaluates existing marketing activities. That creates a foundation for the following in-person interviews with visitors of the selected match and mainly for a quantitative survey researching fans' opinions on the marketing communications of the club. The output of the thesis is a proposed club’s communication campaign for the upcoming season.
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Moc, Filip. "Vestavěný systém pro automatizaci chovné stanice akvarijních ryb." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2018. http://www.nusl.cz/ntk/nusl-385996.

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This master's thesis is concerned about embedded system for automatic water renewal in aquariums. Besides water renewal system also controls lights and ventilation in aquarium fish breeding station. In this report you will find informtions about technology relevant to automation of aquarium fish breeding station. Also you will find here informations about design and implementation of my specific final working solution.
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Book chapters on the topic "HC-Pro"

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"HC-Pro." In Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_7385.

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"Chung and Ohm triterpene alcohols including 4,4'-dimethylsterols, which is germ and aleurone fractions (Table 25). Germs are the substantially higher than those in corn oil and wheat germ richest source of lipids among all cereal grain fractions, oil [126,127,129]. even though they are relatively small fractions of grain Kuroda et al. [128] analyzed SE, S, SG, and ASG of kernels. The weight percentage of germ is 10-14% of corn, bran separately (Table 22). The 4-methylsterols and triter-8-12% of sorghum, 7% of oats, 2-4% of wheat and 1-2% pene alcohols with 4,4'-dimethylsterol were found along of rice kernel weights. with the 4-demethylsterols in SE and S but not in SG or Lipids are unevenly distributed in grain fractions, and ASG. The principal FA components of SE were linoleic lipid distribution differs among grains (Table 25). In corn (58.3%), oleic (30.4%), and palmitic (7.4%) acids, where-kernels, 73-85% of the lipid is distributed in the germ frac-as those of ASG were linoleic (42.5%), palmitic (29.9%), tions [137,138], whereas in rye, triticale, and wheat ker-and oleic (22.7%) acids [97]. The principal 4-demethyl-nels, 34-42% of the lipid is in the germ fraction [78]. The sterols of all flour sterol lipids (SE, S, SG, and ASG) and corn lipid distribution is quite similar despite the genetic bran oil were (3-sitosterol, campesterol, and stigmasterol differences in strains. The H51 is inbred; LG-11 is a three-(Table 22). The principal 4-monomethylsterols of bran oil way cross hybrid forage corn; both the waxy maize and and sterol lipids (SE and S) were gramisterol and citrosta-amylomaize are endosperm mutants. Amylomaize is also a dienol, and the principal 4,4'-dimethylsterols were 24-high-oil strain [9]. Price and Parsons [139] reported that methylenecycloartanol and cycloartenol. the hulless barley (Prilar) and the hulless oat (James) lipids Mahadevappa and Raina [129] reported the total sterol were distributed mainly in the bran-endosperm fractions lipid content as 149 mg in 100 g finger millet including 13 (Table 26). mg SE, 91 mg S, 25 mg SG, and 20 mg ASG. The major Among oat groat fractions, FL and TL were highest in FA, totaling 85-90%, were the same in both esterified the scutellum and BL were highest in embryonic axis sterols, but the proportions varied: palmitic, oleic, and (Table 27). Both red and white proso millet fractions con-linoleic acids comprised 24, 49, and 17% in SE and 43, 36, tained similar lipid contents except for the bran FL con-and 7% in ASG. All flour sterol lipids in finger millet con-tents, which were somewhat higher in the white than those tained 80-84% (3-sitosterol with the reminder being stig-in the red proso millets [33]. masterol [129]. The starch composition influences the lipid content of The 4-demethylsterols compose 87-98% of the total starch. High-amylose barley and corn starch contained sterols in both corn oil and wheat germ oil (Table 23). The higher FFA and LPL contents than waxy and normal types 4-demethylsterol contents were 1441 and 1425 mg in 100 (Table 28). Waxy-type starch contained lower lipid content g of corn oil and wheat germ oil, respectively [130]. The 13-than normal starchs of barley, corn, and rice (Table 28). sitosterol and campesterol are the major 4-demethylsterols in both corn oil and wheat germ oil. The major 4-B. Lipid Compositions in Various monomethylsterols are gramisterol and citrostadienol. In Grain Fractions addition, obtusifoliol is another major component in corn jor 4,4'-dimethylsterols are 24-methylenecy-Since the cereal lipid compositions are too complex to oil. The ma compare for all grains in one section, each will be dis-cloartanol and cycloartenol in corn and wheat germ oils. A cussed separately. substantial amount of 13-amyrin is present in wheat germ oil (Table 23). 1. Barley Long-term storage or heat treatment of flour [132] pro-The average compositions of NL and PL for two varieties, duces sitosterol oxides. The production of sitosterol oxides Kearney (winter type) and Prilar (spring type), are given in was investigated using wheat flour [132]. The 7-hydroxy-Table 29. In barley, like other cereal grains, NL are the ma-sitosterol of wheat flour lipid increased from 25.4 ppm af-jor class of NSTL (Table 3) and over one half of NL are TG ter 2 months storage to 245.0 ppm after storage of 36 (Table 29). The NL also contains 9.8% free sterols, 4.4% months (Table 24). SE, and 5.7% HC [139]. The two major classes of PL are PC and LPC (Table 29). The FA composition varies among lipid classes. The major FA is 18:2 for all classes except for IV. LIPIDS IN STRUCTURAL PARTS PG and PA. The "others" in Table 29 include relatively OF GRAINS small quantities of the other minor FA (12:0, 14:0, 16:1 A. Lipid Contents in Various and 20:0) [142,143]. Grain Fractions The NSL contents and compositions in hulless barley (Prilar) fractions and their FA compositions of NL, GL, Endosperms are the major fractions of all cereal grains, and PL are given in Table 30. The FA composition differs and yet their lipid contents are significantly lower than depending on the structural parts of the barley kernels." In Handbook of Cereal Science and Technology, Revised and Expanded. CRC Press, 2000. http://dx.doi.org/10.1201/9781420027228-45.

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