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Rip, Diane. "Sample preparation methods and molecular based detection for the rapid isolation and identification of Listeria monocytogenes in food samples." Thesis, University of the Western Cape, 2006. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1346_1255007553.
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Listeria monocytogenes is a Gram-positive bacterium responsible for listeriosis, a food-borne disease, which may result in severe illness and possible death. The importance of L. monocytogenes as a food-borne pathogen has been recognized since the 1980's when a correlation between the cunsumption of contaminated foodstuffs and human listeriosis outbreaks was observed. Listeriosis occurs with the ingestion of contaminated foods. The aim of this study involved developing DNA based methods to aid the food industry for the fast detection of L. monocytogenes in food products. Therefore assays were developed in such a way that they will have potential applications in the food idustry.
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Jarvis, Sandra Marie. "The application of immunology to food science, two studies : production of monoclonal antibodies (Mabs) specific for an enteropathogenic E. coli (EPEC) ; development of an enzyme-linked immunosorbent assay (ELISA) for [Beta]-N-acetylglucosaminidase (NAGase)." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/28947.
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Two hybridoma clones, labelled 4D10 C1 and 2H4 H12, produced monoclonal antibodies which recognized the outer membrane of an enteropathogenic Escherichia coli (EPEC) 0142:K86:H6 in an enzyme-linked immunosorbent assay (ELISA) and the whole cell in an immunofluorescence assay. Large scale production of the monoclonal antibodies was accomplished through ascites production in balb/c mice. Purification of the ascites fluid was achieved by gel filtration and ion exchange chromatography. Isotyping of the purified fractions showed 4D10 C1 to be an IgG2 and 2H4 H12 an IgM. These monoclonal antibodies were screened by immunofluorescence assay against several pathogenic and nonpathogenic
strains of E.coli in addition to other Enterobacteriaciae. Results of the screening showed these antibodies to be specific for the E.coli serotype to which they were raised. Minimal cross-reactivity with other Enterobacteriaceae was observed.
In a separate and concurrent project, the use of an ELISA capable of detecting ß-N-acetylglucosaminidase (NAGase) was examined. White Leghorn hens were injected with commercially prepared bovine NAGase. Eggs were collected and the immunoglobulin fraction separated from the egg yolk by polyethylene glycol precipitation followed by ion exchange on a DEAE-Sephacel column. The use of the purified immunoglobulins was examined in a sandwich, double-sandwich and a competitive ELISA. A statistically significant standard curve for the detection of NAGase was successfully derived using a double-sandwich ELISA when rabbit immunoglobulin was used to coat the microwell plates. This assay was used to measure the NAGase concentration in press juice and fish extract of fresh and frozen salmon muscle samples. The ratio of the NAGase concentration in the press juice to the total NAGase concentration was compared. No significant difference was found between the calculated concentration ratios of the fresh muscle samples and samples frozen for 1 week at -20°C.Land and Food Systems, Faculty of
Graduate
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Qin, Yanyan. "Health risk assessment of POPs and heavy metals in Hong Kong residents based on their concentrations in selected food items and different human tissues (blood plasma and adipose tissues)." HKBU Institutional Repository, 2010. http://repository.hkbu.edu.hk/etd_ra/1234.
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Chan, Kit Yan. "Dietary exposure, human body loadings, and health risk assessment of persistent organic pollutants at two major electronic waste recycling sites in China." HKBU Institutional Repository, 2008. http://repository.hkbu.edu.hk/etd_ra/943.
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Shange, Nompumelelo. "Contamination of game carcasses during harvesting and slaughter operations at a South African abattoir." Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/98112.
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Thesis (MSc Food Sc)--Stellenbosch University, 2015.ENGLISH ABSTRACT: The consumption of game meat and its by-products is increasing locally and internationally. The increase in consumption requires research that is focused on the microbiological quality of game meat. The harvesting and slaughter process of springbok carcasses revealed the presence of bacterial contamination. Swab samples taken after skinning portrayed a presence of Escherichia coli (E. coli) and Enterobacteriaceae. Springbok carcasses swabbed after chilling indicated aerobic bacteria, Clostridium spp. and lactic acid bacteria. In contrast, swab samples taken at the evisceration’s incision area tend to be lower in counts when compared to swab samples taken after skinning and after chilling. Bacterial contamination was linked to poor hygienic practices during the harvesting and slaughter process. Results showed a need for the investigation of the slaughter process. To evaluate the slaughter process’s impact on the microbial quality of game carcasses, black wildebeest (Connochaetes gnou) carcasses were sampled throughout the slaughter process. Before skinning, aerobic bacteria, Enterobacteriaceae, and E. coli were enumerated from hide samples, counts ranged from 0.92 to 7.84 log cfu/g. after skinning, bacterial counts ranged from 0.93 to 6.12 log cfu/g and further decreased after chilling. Clostridium spp. counts increased after skinning, however, statistical analysis detected no significant differences between counts. Salmonella spp. was not detected. The results indicate that bacterial contamination does occur during the slaughter process. Hygienic status during the production of game meat products was also determined. Bacterial counts from raw game meat ranged from 2.37 to 5.37 log cfu/g. Counts as high as 6.16 log cfu/g were enumerated from retail products. Aerobic plate counts (APC) from ≤ 2.62 log cfu/cm2 to ≤ 6.3log cfu/cm2 were enumerated from surfaces, hands and equipment during production. Results highlighted the inefficiency of cleaning procedures and revealed that contaminated meat can allow for bacterial contamination. To determine if muscle pH influences colour stability and microbial spoilage of game meat, normal (n=6) and dark, firm and dry (DFD) (n=6) black wildebeest Longissimus thoracis et lumborum (LTL) muscles were studied. pH affected colour, as initial (day 0) L*,a*,b*,C* and Hab values from Normal pH samples were significantly higher than values reported for DFD samples. Initial APC and Enterobacteriaceae counts from samples with Normal pH were not significantly different from counts reported for DFD samples. Initial contamination was linked to the harvesting and slaughter process. Further refrigeration (5±1ºC) for 12 days in an aerobic environment and analyses of samples every third day revealed that pH did not affect lightness and brownness as L* and b* values for DFD samples did not significantly differ overtime, the same trend was seen for samples with Normal pH. Normal pH samples showed a significant increase in a* and C* values until day 12, whilst Hab values decreased until the 12th day. The same trend was seen for a* and C* values for DFD samples until the 9th day as on the 12th day values increased. Similarly, Hab values for DFD samples decreased until the 9th day, then increased on the 12th day. Using the microbial spoilage limit of 6 log cfu/g, it was seen that DFD meat reached this limit earlier than samples with Normal pH. Overall, the study provides baseline information on the microbiological quality of game meat harvested in South Africa and slaughtered at a South African abattoir.
AFRIKAANSE OPSOMMING: Die plaaslike en internasionale verbruik van wildsvleis en wildsvleisprodukte is aan’t toeneem. Hierdie toename in verbuik vereis navorsing wat gefokus is op die mikrobiese kwaliteit van wildsvleis. Die oes-en slagproses van springbok karkasse het die teenwoordigheid van bakteriese kontaminasie aan die lig gebring. Monsters geneem met ʼn depper na afslag van karkasse het ʼn teenwoordigheid van Escherichia coli (E. coli) getoon. Springbok karkasse wat getoets is na verkoeling het hoë vlakke van die aërobiese bakterium Clostridium spp. en van melksuurbakterieë getoon. In teenstelling hiermee is getalle laer rondom die ontweidings insnyding. Bakteriese kontaminasie was gekoppel aan swak higiëne gedurende die oes- en slagproses. Hierdie resultate het ʼn ondersoek van die slagproses aangemoedig. Om die impak van die slagproses op die mikrobiese kwaliteit van wildskarkasse te evalueer, is monsters regdeur geneem van swartwildebees (Connochaetes gnou). Getalle van aërobiese bakterieë, Enterobacteriaceae, en E. coli was bepaal op vel monsters voor afslag; getalle het gewissel tussen 0.92 en 7.84 log cve/g. Getalle van bakterieë na afslag het gewissel tussen 0.93 en 6.12 log cfu/g, en het verder afgeneem na verkoeling. Clostridum spp. het toegeneem na afslag, maar statistiese analises het geen beduidende verskille getoon nie. Monsters het negatief getoets vir Salmonella spp. Die resultate toon aan dat bakteriese kontaminasie wel plaasvind gedurende die slagproses. Die higiëniese status gedurende die produksie van wildsvleis is ook vasgestel. Bakteriegetalle van rou wildsvleis het gewissel tussen 2.37 log cve/g en 5.37 log cve/g. Getalle van handelsprodukte het getalle getoon van soveel as 6.16 log cve/g. Aërobiese plaat telling tussen ≤2.62 cve/cm2 en ≤ 6.3log cve/cm2 is vasgestel vanaf oppervlakte, hande en toerusting gedurende produksie. Resultate beklemtoon die ondoeltreffendheid van skoonmaakprosedures en wys dat aangetaste vleis bakteriese kontaminasie kan toelaat. Om te bepaal of die kleurstabiliteit en mikrobiese bederf van wildsvleis geaffekteer word deur spiere se pH, is normale (n=6) en donker, ferm, en droë (DFD) (n=6) Longissimus thoracis et lumborum (LTL) spiere van die swartwildebees bestudeer. Kleur was geaffekteer deur vleis pH, siende dat die aanvanklike waardes (dag 0) vir L*, a*, b*, C* en Hab aansienlik hoër was vir monsters met normale pH as DFD monsters. Aanvanklike getalle van aërobiese plaat telling en Enterobacteriaceae telling van monsters met Normale pH het nie beduidend verskil van DFD monsters nie. Aanvanklike besmetting was gekoppel aan die oes- en slagproses. Verdere verkoeling (5±1ºC) vir 12 dae in ʼn aërobiese omgewing en analise van monsters wys dat pH nie ligtheid en bruinheid affekteer nie; waardes vir L* en b* vir DFD monsters het nie beduidend verskil oor tyd nie. Dieselfde geld vir monsters met Normale pH. Monsters met Normale pH het ʼn beduidende toename in a* en C* getoon tot en met dag 12, terwyl waardes vir Hab afgeneem het tot en met dag 12. Dieselfde patroon is waargeneem by waardes vir a* en C* vir DFD monsters tot en met dag 9, terwyl dit toegeneem het op die 12de dag. Soortgelyk het Hab waardes vir DFD monsters afgeneem tot n met dag 9, en toegeneem op die 12de dag. Dit is ook gevind dat DFD vleis die limiet vir mikrobiese bederf (6 log cve/g) vroeër bereik as monsters met Normale pH. Die studie voorsien basis inligting oor die mikrobiese kwaliteit van wildsvleis wat geoes is in Suid Afrika, en geslag is by Suid Afrikaanse slagpale.
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Cawthorn, Donna-Maree. "Detection, identification and live/dead differentiation of the emerging pathogen Enterobacter sakazakii from infant formula milk and the processing environment." Thesis, Stellenbosch : Stellenbosch University, 2007. http://hdl.handle.net/10019.1/21603.
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Thesis (MSc)--University of Stellenbosch, 2007.ENGLISH ABSTRACT: The World Health Organisation (WHO) estimates that at least 75% of infants receive infant formula milk (IFM) either entirely or in conjunction with breast milk during the first four months after birth. The presence of the emerging pathogen Enterobacter sakazakii in IFM has been associated with rare but fatal cases of neonatal infections and deaths. There is thus a need for accurate methods for the rapid detection of E. sakazakii in foods. At present, the methods used to detect and identify this micro-organism are inadequate, controversial and contradictory. The aim of this study was to determine the most suitable method for E. sakazakii detection after evaluation of the currently available methods. A further aim was to optimise a polymerase chain reaction (PCR) method for the detection of only viable E. sakazakii cells utilising the DNA-intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA). The Food and Drug Administration (FDA) method for E. sakazakii detection was utilised to select 50 isolates from IFM and 14 from the environment, regardless of colony appearance. These isolates were identified by sequencing a 1.5 kilobase (kb) fragment of the 16S ribosomal DNA (rDNA) and by using the National Centre for Biotechnological Information (NCBI) database to confirm the closet known relatives. Seven of the 50 (14%) IFM isolates and six of the 14 (43%) environmental isolates were identified as E. sakazakii. The methods that were evaluated for accuracy in detecting and identifying these E. sakazakii isolates included yellow pigment production on tryptone soy agar (TSA), chromogenic Druggan-Forsythe-Iversen (DFI) and Enterobacter sakazakii (ES) agars and PCR using six different species-specific primer pairs described in the literature. The suitability of the FDA method was lowered by the low sensitivity, specificity and accuracy (87%, 71% and 74%, respectively) of using yellow pigment production for E. sakazakii identification. DFI and ES agars were shown to be sensitive, specific and accurate (100%, 98% and 98%, respectively) for the detection of E. sakazakii. The specificity of the PCR amplifications was found to vary between 8% and 92%, with Esakf and Esakr being the most accurate of the primer pairs evaluated. The current FDA method for E. sakazakii detection requires revision in the light of the availability of more sensitive, specific and accurate detection methods. Based on the results obtained in this study, a new method is proposed for the detection of E. sakazakii in food and environmental samples. This proposed method replaces the culturing steps on violet red bile glucose agar (VRBGA) and TSA with culturing on chromogenic DFI or ES agar. For identification and confirmation of presumptive E. sakazakii isolates, the oxidase test, yellow pigment production and API biochemical profiling is replaced by DNA sequencing and/or species-specific PCR with the most accurate primer pair (Esakf and Esakr). The amendments to the current FDA method will reduce the time to detect E. sakazakii from approximately 7 days to 4 days and should prove to be more sensitive, specific and accurate for E. sakazakii detection. In this study, a novel PCR-based method was developed which was shown to be capable of discriminating between viable and dead E. sakazakii cells. This was achieved utilising the irreversible binding of bacterial DNA to photo-activated PMA or EMA in order to prevent PCR amplification from the dead cells. At concentrations of 50 and 100 μg.ml-1, PMA completely inhibited PCR amplification from dead cells, while causing no significant inhibition of the PCR amplification from viable cells. EMA was equally effective in preventing PCR amplification from dead cells, however, it also inhibited PCR amplification from viable cells. PMA-PCR in particular, will be useful for assessing the efficacy of processing techniques, as well as for monitoring the resistance, survival strategies and stress responses of E. sakazakii. This will be an important step in the efforts to eliminate E. sakazakii from food and food production environments.
AFRIKAANSE OPSOMMING: Die Wêreld Gesondheidsorganisasie (WGO) beraam dat ten minste 75% van alle babas net baba formule melk (BFM) of BFM in kombinasie met moedersmelk in die eerste vier maande na geboorte kry. Die teenwoordigheid van die voortkomende patogeen Enterobacter sakazakii in BFM is al geassosieer met skaars maar noodlottige gevalle van neonatale infeksies en sterftes. Akkurate metodes word dus benodig vir die vinnige deteksie van E. sakazakii in voedsel. Die metodes wat huidiglik gebruik word vir die deteksie en identifikasie van hierdie mikroörganisme is onvoldoende, kontroversieël en teenstrydig. Die doel van hierdie studie was om die beste metode vir die deteksie van E. sakazakii te bepaal, na 'n evaluasie van die metodes wat huidiglik beskikbaar is. 'n Verdere doel was om 'n polimerase ketting reaksie (PKR) metode vir die deteksie van slegs lewensvatbare E. sakazakii selle te optimiseer deur gebruik te maak van die DNSbindende kleurstowwe, etidium mono-asied (EMA) en propidium mono-asied (PMA). Die Voedsel en Medisyne Administrasie (VMA) se metode vir E. sakazakii deteksie is gebruik om, ongeag van die kolonie kleur, 50 isolate vanuit BFM en 14 isolate vanuit die omgewing te kies. Hierdie isolate is geïdentifiseer deur die DNS volgorde van 'n 1.5 kilo-basis (kb) fragment van die 16S ribosomale DNS (rDNS) te bepaal en die Nationale Sentrum vir Biotegnologiese Informasie (NSBI) databasis te gebruik om die mees verwante spesie te bevestig. Sewe van die 50 (14%) BFM isolate en ses van die 14 (43%) omgewings isolate is geïdentifiseer as E. sakazakii. Die metodes wat geëvalueer is in terme van akkuraatheid vir deteksie en identifikasie van hierdie E. sakazakii isolate het PKR met ses verskillende spesie-spesifieke peiler pare soos beskryf in die literatuur, geel-pigment produksie op triptoon soja agar (TSA) en chromogeniese Druggan-Forsythe-Iversen (DFI) en Enterobacter sakazakii (ES) agars ingesluit. Die geskiktheid van die VMA metode is verlaag deur die lae sensitiwiteit, spesifisiteit en akkuraatheid (87%, 71% en 74% onderskeidelik) van geel pigment produksie vir E. sakazakii identifikasie. Chromogeniese DFI en ES agars was sensitief, spesifiek en akkuraat (100%, 98% en 98% onderskeidelik) vir die identifikasie van E. sakazakii. Die spesifisiteit van die PKR produkte het gewissel tussen 8% en 92%, en Esakf en Esakr is as die akkuraatste geëvalueerde peiler paar geidentifiseer. Die huidige VMA metode vir E. sakazakii deteksie vereis hersiening aangesien meer sensitiewe, spesifieke en akkurate deteksiemetodes voortdurend beskikbaar word. 'n Nuwe metode, gebaseer op die resultate van hierdie studie, word voorgestel vir die deteksie van E. sakazakii in voedsel- en omgewingsmonsters. Die voorgestelde metode vervang die kwekingsstap op violet rooi gal glukose agar (VRGGA) en TSA deur kweking op chromogeniese DFI of ES agars. Verder word die oksidase toets, geel pigment produksie en API biochemiese profiele van vermoeidelike E. sakazakii isolate vervang deur DNS volgorde bepaling en/of spesie-spesifieke PKR met die mees spesifieke peiler paar (Esakf and Esakf) vir die identifikasie en bevestiging van E. sakazakii. Die voorgestelde wysigings van die VMA metode sal die tydsduur van E. sakazakii identifikasie van 7 dae na 4 dae verminder, en behoort ook meer sensitief, spesifiek en akkuraat te wees vir die deteksie van E. sakazakii. 'n Nuwe PKR-gebaseerde metode wat tussen lewensvatbare en dooie E. sakazakii selle kan onderskei is in hierdie studie ontwikkel. Dit is bereik deur die onomkeerbare binding van bakteriële DNS aan lig-geaktiveerde EMA of PMA om die PKR amplifisering van dooie selle te voorkom. Konsentrasies van 50 en 100 μg.ml-1 PMA het PKR amplifikasie heeltemal geïnhibeer, terwyl geen inhibisie van lewensvatbare selle bespeur kon word nie. EMA was ook suksesvol in die voorkoming van die PKR amplifikasie van dooie selle, alhoewel daar ook 'n mate van DNS inhibisie was tydens die amplifikasie van lewensvatbare selle. PMA-PKR kan ook van nut wees vir die assessering van die doeltreffendheid van prosesseringstegnieke, en ook vir die waarneming van die weerstandigheid, oorlewingsstrategieë en stresresponse van E. sakazakii. Dit sal 'n belangrike stap wees in pogings om E. sakazakii van voedsel en voedsel produksieomgewings te elimineer.
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Nhlapo, Nthabiseng. "Hygiene and nutritional content of the National School Nutrition Programme in Bloemfontein, South Africa." Thesis, [Bloemfontein?] : Central University of Technology, Free State, 2013. http://hdl.handle.net/11462/180.
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Thesis (M. Tech. (Environmental Health)) -- Central University of technology, Free State, 2013Malnutrition and concomitant infections are major contributing factors to child morbidity and mortality in developing countries such as South Africa. Globally, children benefiting from school feeding programmes are generally from communities with low socio-economic statuses. The meals provided through feeding schemes, such as the National School Nutrition Programme (NSNP) in South Africa, are aimed at significantly supplementing the beneficiaries’ daily energy and nutrient requirements. The possible nutrient deficiency of meals provided through such programmes compromises the nutritional well-being of children, promotes malnutrition and renders children more susceptible to infectious diseases. Furthermore, illness, particularly caused by diarrhoeal and infectious diseases, is a major factor contributing to child malnutrition as the human body is unable to efficiently digest foods and absorb nutrients during illness. Therefore, the microbiological safety of the foods served to children via feeding schemes is essential. In an effort to contribute towards the safety and wholesomeness of foodstuffs served through the NSNP, the present study was conducted with a view to assess the nutritional quality and safety of the foodstuffs. The knowledge, attitudes and practises (KAP) of food handlers and NSNP representatives/committee members at the schools were also investigated in order to assess origins of potential food contamination. Data collected via nutritional analyses of meals served to school children were compared to the nutrient-based standards set by the United Kingdom as guidelines for an average school lunch. The carbohydrate, energy, calcium and zinc contents of the school meals were below the standards, the majority of the meals met the protein and iron standards and all meals complied with the standards for lipid and vitamin C contents. During the microbiological analysis study, preparation surfaces yielded higher counts of all detected organisms (total coliforms, Escherichia coli, Staphylococcus aureus and yeasts and moulds) compared to the hands of food handlers. Therefore possible sources of contamination may be foodstuffs, animal pests and environmental elements such as dust. However, significant differences of surface microbial counts could not be established (P > 0.05) and thus cross-contamination may have resulted among surfaces, possibly augmented by shortfalls in cleaning regimes. The majority of the participants of the questionnaire survey reportedly washed their hands and cleaned all surfaces several times during the day with water and detergent. In addition, a disinfectant was used by some of the food preparers during cleaning. Furthermore, participants stated that their aprons were washed daily. These claims were in agreement with the findings of the surface microbial study with regards to the hands, however, they did not match the findings of the preparation surfaces which contained the highest counts of total coliforms, E. coli and S. aureus of the three surfaces analysed, and aprons responsible for the highest yeast and mould counts. Improper storage and food preparation methods, such as the application of high temperatures, excessive exposure to UV light and oxygen, and high moisture conditions, may result in the deterioration of nutrients. Storage conditions which may permit pest infestation may also increase the risk of food-related illnesses and risk could be further elevated by application of improper cleaning and sanitation practices. In order for the NSNP to operate effectively, it is essential that the national and provincial Departments of Basic Education, which are responsible for the overall administration of the NSNP, and schools’ personnel function in an integrated manner through support structures and effective communication. Infrastructural limitations (lack of proper kitchen facilities and ventilation) and shortage of resources while administering the NSNP were the main challenges observed during the present study. Due to these hurdles, adhering to food safety practices and maintaining nutritional quality of foods may be a challenge. Continuous training in personal and general hygiene is also a necessity in preserving food safety. Furthermore, nutritional quality of foodstuffs may be preserved through proper storage practices and application of preparation methods which minimize loss of nutrients.
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Palcich, Gabriela. "Ocorrência de Enterobacter sakazakii no ambiente de lactários de Maternidades da Grande São Paulo." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/9/9131/tde-31012018-174253/.
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Enterobacter sakazakii é um bacilo Gram-negativo, pertencente à família Enterobacterieceae. Este microrganismo vem ganhando a atenção das autoridades de saúde pública ao redor do mundo, não tanto pela morbidade, que é baixa, mas pela elevada taxa de mortalidade que varia de 40-80%. O patógeno afeta principalmente recém-nascidos de baixo peso e bebês com até seis meses de idade. Em comum, estas crianças têm o fato de serem alimentadas com fórmula infantil desidratada, a base de leite. Em nosso país ainda não existem muitos estudos sobre a ocorrência deste patógeno em fórmulas infantis, nem no ambiente de preparo das mesmas. O objetivo deste trabalho foi avaliar as condições de produção de mamadeiras para recém-nascidos em maternidades da Grande São Paulo, além de determinar a população de E. sakazakii em fórmulas infantis desidratadas e reidratadas. A população de Enterobacteriaceae e a presença de E. sakazakii também foram avaliadas em amostras ambientais, de utensílios e mão de manipuladores. Avaliou se ainda o comportamento do patógeno em fórmula infantil reidratada simulando as condições de oferecimento aos bebês. Coletou-se amostras de três hospitais maternidades diferentes (A-escola/B-público/C-particular) e analisou-se a presença de E. sakazakii usando método ISO. Para fórmulas desidratadas e reidratadas usou-se a mesma metodologia e a técnica de número mais provável (NMP). A população de Enterobacteriaceae foi determinada usando-se PetrifilmTM 3M. E. sakazakii foi detectada em duas amostras do Hospital A (na sobra da mamadeira que voltou do berçário e de uma amostra da lata lacrada da fórmula infantil desidratada. A população nesta amostra foi de 0,03 NMP/100g.) No Hospital B, foi detectada em apenas uma amostra (na esponja de lavagem das mamadeiras contaminadas). No Hospital C, E. sakazakii não foi detectada nas amostras analisadas. Quanto à população de Enterobacteriaceae nos lactários, observou-se uma variação, sendo que as amostras colhidas no hospital C foram as que apresentaram populações mais elevadas. As cepas de E. sakazakii isoladas apresentaram comportamento similar àquele da cepa padrão, ocorrendo um aumento de 2 log na população do patógeno quando simulou-se as condições de serviço das fórmulas, via naso-gástrica, aos bebês nos berçários.Enterobacter sakazakii is a bacillus belonging to the Enterobacteriaceae family. It is considered an opportunistic pathogen that has been gaining attention from health authorities all over the world. While morbidity associated with this bacterium is low, mortality rates can range from 40-80%. The pathogen affects mainly low-birth-weight neonates (first 28 days), but babies less than 6 month old are also at risk. Powdered infant formula has been incriminated as the possible source of the microorganism to the infected babies. ln Brazil, as in several other countries, there is scarce information regarding the incidence of E. sakazakii in powdered infant formula, in reconstituted formula, and in milk kitchens areas in hospitals. The objective of this study was to evaluated the presence of E. sakazakii in the environment, utensils, handlers, powdered and rehydrated infant formula from milk kitchens from different maternity wards in Sao Paulo, Brazil. Moreover, it was evaluated the behavior of the pathogen in rehydrated infant formula. Samples were collected from 3 hospitals maternities (A-school/B-public/C-particular) and analyzed for E. sakazakii using the ISO method. For formula (powdered or rehydrated) the MPN technique was used. Enterobacteriaceae population was determined using PetrifilmTM 3M. E. sakazakii was found in one unopened formula can collected from Hospital A (0,03 MPN/100g), although the pathogen could not be detected in other cans from the same lot. E. sakazakii was also found in leftovers from one nursing bottle from the same hospital and from one cleaning sponge from Hospital B. E. sakazakii was not detected in none of the samples from Hospital C. A variation in Enterobacteriaceae population in milk kitchens was observed. Samples collected in Hospital C presented the highest population. Isolated strains of E. sakazakii presented similar behavior to standard strains, When spiked in rehidrated infant formula. A 2 log increase in the population of the pathogen was observed when simulating the conditions of formula administration to the babies by naso-gastric tubing.
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Costa, Estela, and University of Lethbridge Faculty of Arts and Science. "Modulation of the immune system in the mammalian intestine as an alternate explanation for the action of antimicrobial growth promoters / Estela Costa." Thesis, Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2010, 2010. http://hdl.handle.net/10133/2627.
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The novel hypothesis that antimicrobial growth promoters (AGP) function by
modulating the mammalian immune system was tested. Sampling methods to
characterize the mucosa-associated microbiota of the murine intestine by terminal
restriction fragment length polymorphism (T-RFLP) analysis indicated that direct plug
extraction was superior to wash methods. Using T-RFLP analysis, non-therapeutic
administration of chlortetracycline (CTC) and sulfamethazine to beef cattle did not affect
the composition of bacterial communities associated with intestinal mucosa and in
digesta, with exception of those associated with mucosa of the proximal jejunum.
Similarly, oral administration of non-therapeutic concentrations of CTC did not affect the
mucosa-associated microbiota of the murine intestine. Oral administration of nontherapeutic
concentrations of CTC prevented weight loss, reduced pathologic changes,
modulated transcription levels of inflammatory cytokines in C. rodentium-infected mice,
and did not consistently affect the colonic microbiota. These findings support the
hypothesis that AGP primarily function by modulating the intestinal immune system.xiv, 160 leaves ; 29 cm
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Spooner, Ashley. "The mercury contamination in fish and the subsequent public health effects." [Denver, Colo.] : Regis University, 2006. http://165.236.235.140/lib/ASpooner2007.pdf.
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Senior Honors thesis--Regis University, Denver, Colo., 2007."May 2006"--T.p. Date of submission on Certification of Authorship page: 4/16/07. Title from PDF title page (viewed on June 26, 2007). Includes bibliographical references.
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Pillsbury, Laura Anne. "Food cultures, total diet studies and risk management implications for global food policy and public health /." Connect to this title, 2008. http://scholarworks.umass.edu/theses/157/.
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Li, Chun-wai, and 李震維. "Systematic review on fast food consumption and adolescent." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45173035.
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Zhao, Yinge. "Risk assessment of perfluorinated compound (PFC) contamination and their effects on animal reproductive health." HKBU Institutional Repository, 2012. https://repository.hkbu.edu.hk/etd_ra/1416.
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Xu, Weiguang. "Risk assessment of dietary exposure to PCDD/PCDFs, DL-PCBs and NDL-PCBs of Hong Kong residents." HKBU Institutional Repository, 2012. https://repository.hkbu.edu.hk/etd_ra/1407.
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Tsang, Hin Long. "Persistent organic pollutants in foodstuffs and human samples from Hong Kong." HKBU Institutional Repository, 2008. http://repository.hkbu.edu.hk/etd_ra/873.
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Uddin, G. M. Saleh. "Groundwater contamination by arsenic in Bangladesh : causes, consequences and solutions." Title page, table of contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09ENV/09envu18.pdf.
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Klingenberg, Sanette. "Standards for the hand hygiene of food handlers / Sanette Klingenberg." Thesis, North-West University, 2008. http://hdl.handle.net/10394/3734.
Full textAbstract:
Globally, investigations into food-borne illnesses show that the majority of cases involve poor hand hygiene of the food handler. The challenge of providing safe food therefore requires new strategies for evaluating cross-contamination of pathogenic micro-organisms on the food handler's hands, which might be detrimental or hazardous to the health of the patient Although food-borne diseases may be multifactorial in aetiology, no standards or evaluation systems, such as an occupational health surveillance programme, are available to monitor and ensure that food is free of pathogens. The formulation and implementation of standards may contribute to ensuring that food handlers comply with hand hygiene practices during food handling. Such practices guarantee that food reaching the patient is safe.
The objectives in this research project originated from the occupational health practice and gave direction of the empirical research project. The literature was reviewed to discover what is currently known concerning the food handlers' hand hygiene during food handling and food-borne illnesses and the theoretical framework gave direction and guidance to the survey design of the empirical research, which was quantitative, explorative, descriptive and contextual in nature. The food handlers from the food preparation sections of the four major healthcare services in Potchefstroom, in the North West Province, South Africa, were the target population and the sampling method was all-inclusive (n=110). Eighty (75.47%) food handlers participated in the research project.
The design entailed three steps. The first was conducted with a questionnaire, to identify the food handlers' compliance with hand hygiene during food handling. The second step involved determining the prevalence of Escherichia coli and Staphylococcus aureus on the food handlers' hands. The results were used for the formulation of standards for the hand hygiene of food handlers.
Finally, recommendations for practice, education and research were made. The implementation of these recommendations could contribute knowledge to the body of nursing and promote good hand hygiene practices in the healthcare service.Thesis (M.Cur.)--North-West University, Potchefstroom Campus, 2009.
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Ali, Haroon. "Protein-phenolic interactions in food." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=32748.
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Our objective was to investigate the mode of interaction between selected food proteins and phenolic compounds. Bovine serum albumin (BSA), bovine beta-lactoglobulin, and soybean glycinin were used with the following phenolic compounds; 3,4,5-trihydroxybenzoic acid (gallic acid), 3,4-dihydroxy cinnamic acid (caffeic acid), p -hydroxycinnamic acid (courmaric acid), and 5,7-dihydroxy 4-methoxy isoflavone (biochanin A). The interaction was investigated using incubation temperatures of 35°, 45° and 55°C at pH 5, 7 and 9. Native and SDS-polyacrylamide gel electrophoresis (PAGE), differential scanning calorimetry (DSC), and Fourier transform infrared (FTIR) spectroscopy were used to identify protein-phenol interactions. Certain phenolic compounds combined with BSA and prevented protein aggregation. In general, the thermal stability of the proteins increased as a result of interaction with phenolic compounds; the most pronounced effect was observed with beta-lactoglobulin in the presence of gallic acid at pH 7. The interaction of the phenols with the proteins resulted in changes in protein secondary structure. (Abstract shortened by UMI.)
19
殷子敬 and Tsz-king Eric Yan. "Studies on food-derived antihypertensive peptides." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B3122541X.
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Ho, King-yan, and 何景欣. "Ecological and human health risks associated with organotin contamination in the marine environment of Hong Kong and Shenzhen, China." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208427.
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Organotin compounds (OTs), in particular tributyltin (TBT) and triphenyltin (TPT), have caused widespread adverse effects on marine organisms ever since their wide application as biocides in 1960s. For instance, TBT can induce the abnormal development of imposex in marine gastropods, and inhibit development and growth in oysters. A mandatory global ban on the use of OT-based antifouling systems, therefore, has been enacted by the International Maritime Organization to minimize their environmental impacts since September 2008. As a result, it is anticipated to see a reduction of OT contamination in the marine environment around the world. This study, therefore, primarily aims to test the hypothesis that there is a reduction of both OT contamination and its associated adverse impact to a common biomonitor, the rock shell Reishia clavigera along the coast in Hong Kong and Shenzhen, China through a series of field-based investigations.
Before the field study, a method was successfully developed to simultaneously quantify the concentrations of six common OTs (i.e., mono-BT, di-BT and TBT; mono-PT, di-PT and TPT) in molluscan tissues using gas chromatography-mass spectrometry. These six compounds coexist in marine environments. They are highly toxic to marine organisms and are able to induce imposex in gastropods like R. clavigera.
First, a territory-wide survey on tissue concentrations of the OTs and imposex status in R. clavigera collected from 28 coastal sites of Hong Kong was conducted in 2010. The results indicated that all female R. clavigera suffered from imposex, and all rock shells contained high TPT concentrations. A probabilistic ecological risk assessment revealed that 17.6% of R. clavigera across all sites were at risk due to exposure to TPT, whereas the risk associated with TBT was relatively low. The same species collected from Dapeng Bay and Daya Bay, Shenzhen also exhibited high tissue concentrations of TPT. Second, R. clavigera transplanted from relatively clean sites to polluted sites for six months showed increases in both imposex status and tissue concentration of OTs, confirming the association between OT contamination and imposex development. Third, a 25-month population dynamics study of R. clavigera showed a limited recruitment of the species in the polluted sites.
To verify if the observed OT contamination was widespread to other marine species in Hong Kong waters, a survey on the tissue concentrations of OTs in 11 local seafood species was conducted. Among them, the tongue sole Paraplagusia blochii had the highest tissue concentration of total OTs, with TPT as the most abundant residue. Potential health risk for consuming this benthic fish species was identified as both hazard quotient of TPT and hazard index exceeded unity.
The overall results demonstrate that the coastal marine environments in this region are still heavily contaminated with OTs especially TPT, and there is no obvious sign of recovery of R. clavigera populations. Hopefully, with further scientific investigations, both Hong Kong and China governments will take appropriate management actions to control the use and release of these priority pollutants with a view to safeguarding the marine ecosystem and human health in this region.HKU 3 Minute Thesis Award, 2nd Runner-up (2013)
published_or_final_version
Biological Sciences
Doctoral
Doctor of Philosophy
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李惟鍵 and Wai-kin Robin Dominic Lee. "Determination of the optimal conditions and additives for the preservation of epigallocatechin gallate in bottled tea drinks." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41712419.
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Phasha, Mmolawa Cynthia. "Health and safety aspects of the use of products from urine-diversion toilets." Diss., Pretoria : [s.n.], 2005. http://upetd.up.ac.za/thesis/available/etd-02262007-183119.
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Shao, Dingding. "Human risk assessment based on mercury contamination in food and environmental martrix at two regions in Guangdong and Zhejiang Provinces." HKBU Institutional Repository, 2011. http://repository.hkbu.edu.hk/etd_ra/1269.
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Brieger, Katharine K. "PCB-Contaminated Food in the Canadian Arctic: Interactions between Environmental Policy, Cultural Values, and the Healthcare System." Scholarship @ Claremont, 2011. http://scholarship.claremont.edu/pomona_theses/95.
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Current Canadian policies do not adequately prevent water contamination or mitigate the effects of existing polychlorinated biphenyl pollution. There is conflict between the Canadian government and the Inuit people over how to approach the pollution problem, arising from a clash of cultural values and traditions. Legislation related to healthcare is an inherently sensitive issue and some measures that seem acceptable to westernized societies are not appropriate for the Inuit. The purpose of this thesis is to answer (a) why polychlorinated biphenyls (PCBs) are in the Arctic, (b) what the effects of PCBs are on the Arctic people, and (c) how healthcare policy can address the issue.
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Luyt, Stanley Arthur. "A study to assess the changes in hygiene of food premises following a specific health education programme." Thesis, Cape Technikon, 1992. http://hdl.handle.net/20.500.11838/800.
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Thesis (Masters Diploma in Technology (Public Health)) -- Cape Technikon, Cape Town, 1992In order to evaluate a health education programme for food handlers at a meat plant, a bakery/confectionery and a catering premises, changes in hygiene were assessed by the bacteriological analysis of swabs for hygiene indicator organisms from food contact surfaces. In this evaluation three phases were established on the basis of bacteriological assessment prior to, during and after the education programme. The first phase involved the establishment of a base line for hygiene indicator organisms prior to the education programme by taking 5 sets of bacteriological swabs over a two month period at each of the three premises, each swab set consisting of 14 swabs of food contact surfaces making a total of 210 swabs. During this time the food hygiene educational needs of the employees were assessed and on this basis a set of three video taped presentations were produced relating respectively to personal hygiene, environmental hygiene and food handling practices. The second phase consisted of the implementation of a health education programme involving consecutive tutorial sessions at one month intervals during which the video taped programme was presented. At this stage a further 5 sets of bacteriological swabs was taken at each of the premises. The third phase involved the assessment of hygiene shortly after completion of the education programme by taking a final 5 sets of bacteriological swabs of food contact surfaces at each of the premises over a further two month period. Statistically significant reductions in a number of the indicator organisms were observed during the course of the study.
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Rodgers, Svetlana. "Development of a bio-preservation method for extended shelf-life cook-chill systems." Thesis, View thesis, 2003. http://handle.uws.edu.au:8081/1959.7/23237.
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Extended shelf-life cook-chill meals can pose a potential risk of botulism if they are subjected to a temperature abuse. Spores of group II non-proteolytic Clostridium botulinum can survive the mild heat treatment typically given to these products and can grow at refrigeration temperatures. To circumvent this safety issue, existing preservation methods can either affect the sensory properties of these foods or damage their image. Therefore, additional natural preservation hurdles are needed. Thus, the aim of this study was to develop a novel bio-preservation method based on the principle of antibiosis between protective cultures (PCs) and C. botulinum. Consequently, the objectives were to select effective anti-botulinal cultures and study their inhibition pattern in microbiological media and foods, identify the conditions for effective inhibition and the nature of the antibiosis. This research demonstrates for the first time that the bacteriocinogenic protective cultures inoculated at high levels had an anti-botulinal effect in a range of commercial cook-chill products, which supported active growth of non-proteolytic C. botulinum. The protocol for commercial application of the protective cultures was developed.
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Kehoe, Sarah. "The effect of a micronutrient-rich food supplement on women's health and nutrient status." Thesis, University of Southampton, 2012. https://eprints.soton.ac.uk/376892/.
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Givan, Ethan. "Selection of Pathogen Surrogates and Fresh Produce Safety: Implications for Public Health and Irrigation Water Quality Policy." TopSCHOLAR®, 2015. http://digitalcommons.wku.edu/theses/1545.
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Foodborne illness continues to be a substantial public health issue in the United States, with fresh produce being one of the leading causes of outbreaks. Understanding routes of contamination of fresh produce and how pathogens survive on plant surfaces is paramount in improving food safety and reducing risk to public health. The objectives of this study were to select environmental E.coli isolates as pathogen surrogates of Salmonella typhimurium and E.coli O157:H7, assess lettuce plant contamination by spray irrigation water, and evaluate a common industry quality control (QC) E.coli strain (ATCC 25922). Selections of E.coli surrogates were made utilizing biofilm and leaf attachment data from lab scale assays. Five surrogates were found to be similar in biofilm formation and leaf attachment capabilities of the pathogens, while the common QC strain was significantly different than Salmonella in both biofilm formation and leaf attachment (p < 0.05). Persistence of surrogates, pathogens and the QC strain on lettuce plants was assessed in greenhouse scale experiments, where it was found that all isolates were above detection levels for 22 days. Die-off rates were calculated for all isolates, with the QC strain having the greatest rate of die-off in the first experiment (k = -4.52) and the second greatest in the second experiment (-2.82) while the pathogens and selected surrogates had statistically similar and lower rates of die-off. Based on this information, current policies concerning the sampling and management of irrigation waters and crops for microbial safety may be insufficient. It is recommended that sampling methods and frequencies be adjusted for irrigation waters and fresh produce, and the use of projected die-off rates not be used for the determination of time intervals needed before a crop is safe to harvest.
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Feliciano, Lizanel. "Shelf-life Extension of Seafood Using Sanitized Ice." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1252965039.
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Sharma, Kuhuk. "Assessment of heavy metal contamination and restoration of soil food web structural complexity in urban vacant lots in two post-industrial cities." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1406232323.
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Wei, Xi. "Environmental screening of endocrine-disrupting chemicals and biological characterization of their effects on reproductive health." HKBU Institutional Repository, 2011. http://repository.hkbu.edu.hk/etd_ra/1225.
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Webster, Jacqui. "Drop the salt! Engaging the food industry and influencing the policy agenda." Thesis, The University of Sydney, 2010. https://hdl.handle.net/2123/29161.
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Background: The physiological daily requirement for salt is low (1-2g) and most populations
around the world are eating much more than this. Excess salt consumption
progressively increases blood pressure levels throughout life, and this is one of the
biggest contributors to cardiovascular diseases worldwide. The evidence in support
of the adverse effect of salt on human health is very strong and widely accepted.
Fortunately there is now increasingly firm evidence demonstrating the health benefits
that can be achieved through salt reduction as well as broad consensus that, for most
countries, implementing a population-wide salt reduction program would be a highly
cost effective way of improving population health. For decades, Australia has been at
the forefront of clinical academic research defining the role of salt in high blood
pressure. However, while there have been repeated calls for action to reduce salt
intakes, there has been little by way of a sustained and coordinated effort to translate
the scientific evidence into a co-ordinated salt reduction program. The work done for
this thesis describes the development, implementation and interim evaluation of the
first concerted effort to establish a national salt reduction initiative in Australia
between 2007 and 2010.
Methods: A review of the available literature and different national approaches to reducing
population salt intake was undertaken in parallel with a series of consultations with
stakeholders in Australia and internationally to inform a broad understanding of what
might be feasible in Australia. Once the initial research and consultation was
complete, implementation strategies were developed. Baseline data were established
for 2008 through the development of a branded food composition database
documenting the salt content of more than 7000 Australian processed foods and
repeated surveys of consumer knowledge and practices related to salt. Programs of
work to engage the food industry and government were prioritized and underpinned
by a targeted media and communications strategy. A series of interim evaluations of
the program were conducted, including an independent stakeholder analysis to
evaluate views on program impact.
Main outcomes: The limited available studies on salt consumption in Australia showed average salt
intakes were way in excess of physiological requirements. While government targets
for recommended individual salt intakes had been in place for some time, there was
no program in place to try to reduce dietary salt in Australia, with only sporadic
industry efforts to reduce salt in selected foods. Baseline surveys on consumer
knowledge and behaviour confirmed individuals were taking little action to reduce
their salt intakes. There was strong stakeholder support for a co-ordinated salt
reduction initiative in Australia. Based on these findings The ‘Drop the Salt!’
campaign was developed to reduce population salt intake through strategies targeting
industry, government and consumers. Leadership was endowed in the Australian
Division of World Action on Salt and Health, a Non-Governmental organisation
supported by a network of professional experts. Based on the experience from other
countries strategies to engage the food industry to refonnulate foods and to advocate
for government leadership were prioritised.
The initial baseline assessment of sodium levels in foods from the branded food
composition database demonstrated the potential for reductions in salt for most food
categories, supporting the need for a sector-wide program. The food groups with the
highest mean sodium content were sauces and spreads (1,283mg/ 100g) followed by
processed meats (846mg/ 100g). Cereal and cereal products (206mg/ 100g) and fruits
and vegetables (21 1mg/ 100g) had the lowest mean sodium content. Good progress
has since been made with the food industry strategy with more than 20 large
companies making commitments or developing detailed action plans to reduce salt in
foods. The media and communications strategy has elevated the importance of salt
amongst stakeholders and the Drop the Salt! campaign has been identified as having
influenced the priorities of the Federal govemment’s Food and Health Dialogue. This
body is leading a national program of food reformulation focussing initially on salt.
However, with targets established for only two food categories so far, in comparison
to about 80 each in the UK and the US, progress is slow. Baseline assessment
showed only a minority of people taking any action to reduce their salt intakes and
changes in consumer behaviour have not been demonstrated. There have been no
improvements to food labelling, although a comprehensive review is currently
underway.
Conclusions and discussion: With only limited resources it has been possible to design and implement a
coordinated five year national salt reduction initiative in Australia drawing on
experience from other countries. A solid baseline from which to monitor progress has
been established and interim evaluations have demonstrated significant progress in a
short timeframe particularly in relation to engaging the food industry in efforts to
reduce salt in foods. Whilst there has also been some progress with government
action, the lack of strong government commitment and leadership is still a significant
barrier to effective program implementation. This is reflected in the progress of the
Food and Health Dialogue which, at the current rate, will take about twenty years to
establish salt targets for all food categories.
The Drop the Salt! campaign has been credited with forging new collaborations
between stakeholders and salt reduction has offered an achievable entry to food
reformulation for many companies and facilitated dialogues across health and
industry sectors that were not previously engaged. While it is not possible to
absolutely attribute cause and effect for all policy outcomes, and an obvious
limitation of this research is that it did not provide a historical context for salt
reduction activities in Australia, the research does nevertheless illustrate the
important role that a small advocacy group can play in influencing the public health
agenda. With the food industry lobbying hard to prevent new regulation and
government complying, advocacy organisations such as the Australian Division of
World Action on Salt and Health will continue to have a central role in holding both
to account. The very large health gains projected from a very modest investment in
centrally implemented salt reduction strategies mean that this is not an issue that can
be allowed once again to fall off the public health agenda.
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Shum, Cheuk-wai, and 沈卓慧. "Systematic review on attention deficit hyperactivity disorder (ADHD) and food additives in children." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48425333.
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Attention-deficit hyperactivity disorder (ADHD) is a developmental disorder of inattention, impulsivity and hyperactivity. It is the most commonly diagnosed psychosocial problems in childhood with continuing impact into adulthood. This systematic review aims to identify the role of food additives, specifically artificial food coloring, in ADHD and its symptoms. PubMed, Sciencdirect, Cochrane Library and Google Scholar databases (1991-April 2012) were searched, which was supplemented by manual searches of the reference lists. Seven randomized or quasicontrolled trials of elimination diet were summarized. Six of these studies showed artificial food coloring was positively associated with ADHD and its symptoms. This review provides some evidence that artificial food coloring plays a role in ADHD and its symptoms. However, the studies reviewed only showed behavioral improvement after eliminating certain type of artificial food coloring, which does not imply artificial food colorings cause ADHD. Additionally, small sample size, inconsistent definition of artificial food colouring, subjectively reported ADHD using various assessment tools, and possibly publication bias limit the generalizability of the findings. If causal, reducing the intake of artificial food coloring could provide an alternative treatment of ADHD in the future. Understanding the mechanism underlying the association may provide insights into the developmental origins of ADHD.published_or_final_version
Public Health
Master
Master of Public Health
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Wagner, Jana Louise. "The relationship of parent and child food choices : influences of a supermarket intervention /." Diss., This resource online, 1990. http://scholar.lib.vt.edu/theses/available/etd-07282008-135613/.
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Leung, Wing-ying, and 梁詠瑩. "Neighborhood deprivation and fast food outlet on adult obesity." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45172912.
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Tam, Chi-ian, and 譚子欣. "Is menu labeling the next step for promoting healthy eating in Hong Kong?" Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45173916.
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JÃnior, Edmilson Pinto de Albuquerque. "The influence of aspects related to health and the environment on the intention of buying organic food." Universidade Federal do CearÃ, 2012. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14402.
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FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgicoFood choices become more meaningful when it is taken into account that they are directly related to health. Likewise, these decisions already reflect aspects regarding environmental correction and it has been demonstrated a growing interest in the ecological responsibility behind consumed food. Organic food is considered healthy and environmentally friendly, then becoming relevant the investigation about these two constructs associated to the shopping intention of consumers. This study sought to investigate how the aspects related to the environmental awareness and the pursuing of health influence the shopping attitudes and intentions of organic food consumers in Cearà market. To this end, it was applied a survey research type and an integrated model was tested by means of the technique known as structural equation modeling (SEM) in order to observe the relations between the constructs concerning the environment, the health and the theory of planned behavior, which analyze the attitudes and intentions of consumers. The results indicate that there is a positive influence of the motivations related to health on the attitudes towards organics, the same occurring to these attitudes on the shopping intentions related to that type of food. Furthermore, it was observed that the aspects regarding environmental concerns do not influence the shopping intentions of organics in market under research. From a managerial point of view, the contributions from this study reside in the comprehension of the determining variables of the shopping behavior of an ascending market, what suggests basis to the development of strategies, by the agents of the productive chain, adequate to the analyzed market.
As escolhas alimentares tornam-se mais significativas quando se leva em conta que estÃo diretamente ligadas à saÃde. De maneira similar, essas decisÃes jà refletem tambÃm aspectos de correÃÃo ambiental e tem-se demonstrado um crescente interesse na responsabilidade ecolÃgica por trÃs dos alimentos consumidos. Os alimentos orgÃnicos sÃo propagados como alimentos saudÃveis e ecologicamente corretos, e tornou-se relevante investigar esses dois construtos associados Ãs intenÃÃes de compra de consumidores. Este estudo buscou investigar de que maneira os aspectos relativos à consciÃncia ambiental e à busca por saÃde influenciam as atitudes e intenÃÃes de compra do consumidor de alimentos orgÃnicos no mercado cearense. Para tal fim, empreendeu-se uma pesquisa do tipo survey e testou-se por intermÃdio da tÃcnica de modelagem de equaÃÃes estruturais (MEE) um modelo integrado no intuito de verificar as relaÃÃes entre os construtos relativos ao meio ambiente, à saÃde e à teoria do comportamento planejado, que analisa as atitudes e intenÃÃes dos consumidores. Os resultados indicam haver uma influÃncia positiva das motivaÃÃes ligadas à saÃde sobre as atitudes em relaÃÃo aos orgÃnicos, e destas nas intenÃÃes de compra desses alimentos. Verificou-se ainda que os aspectos ligados à preocupaÃÃo ambiental nÃo influenciam as intenÃÃes de compra de orgÃnicos no mercado pesquisado. Do ponto de vista gerencial, as contribuiÃÃes deste estudo residem na compreensÃo das variÃveis determinantes do comportamento de compra de um mercado em ascensÃo, o que sugere bases para o desenvolvimento de estratÃgias, por parte dos agentes da cadeia produtiva, adequadas ao mercado analisado.
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Larsson, Christel. "Young vegetarians and omnivores : Dietary habits and other health-related aspects." Doctoral thesis, Umeå universitet, Institutionen för kostvetenskap, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-5.
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In the middle of the 1990s many adolescents became vegetarians. There was concern among adults about whether these new young vegetarians got enough energy and nutrients from their dietary intake. The aim of this thesis was to investigate the prevalence of young vegetarians, the food and lifestyle habits, dietary intake and nutritional status of vegetarian and omnivorous adolescents. The prevalence of adolescents eating a vegetarian school lunch in 124 Swedish secondary schools was investigated by interviewing matrons. Information about prevalence of vegetarians, food and lifestyle habits, of 2041 15-year old students from Umeå, Stockholm and Bergen, was obtained by a questionnaire. The dietary intake and nutritional status of thirty 16-20 year-old vegans were compared with thirty age, sex and height matched omnivores. Five percent of the adolescents (16-20 years) in Sweden were found to eat vegetarian food at school lunch. In Umeå there was a significantly higher prevalence (15.6%) of 15-year-old vegetarians compared with Stockholm (4.8%) and Bergen (3.8%). It was also found that more females than males (15 years old) chose a vegetarian dietary regime. Even though the female vegetarians consumed vegetables significantly more often than the omnivores, the intake (32 times/month) was not as often as might be expected of a vegetarian population. The male vegetarians reported eating vegetables not even once a day (25 times/month). No difference in the consumption frequency of fruits/berries, alcoholic beverages, sweets/chocolates and fast foods was seen between vegetarians and omnivores. However, female vegetarians more often than female omnivores consumed dietary supplements. Furthermore, lifestyle characteristics of vegetarians were similar those of omnivores regarding exercise, use of alcohol and smoking habits. No significant difference in validity of reported energy expenditure or energy and protein intakes was found between vegans and omnivores. Young vegans (16-20 year-olds) were seen to have a higher calculated intake of vegetables, legumes, and dietary supplements and a lower intake of ice creams, cakes/cookies, and candies/chocolate than omnivores. The dietary intake was below the average requirements of riboflavin for 73% of the vegans, vitamin B12 for all vegans, vitamin D for 43% of the vegans, calcium for 77% of the vegans and selenium for all vegans and 43% of the omnivores. If intake of supplements was included the intake of e.g. calcium and selenium was still lower than the average requirements for 67% and 73% of the vegans respectively. Low iron stores were as prevalent among vegans as among omnivores (20% and 23% with low stores) and three vegans had low vitamin B12 concentrations in blood. The findings imply that food and lifestyle habits of young vegetarians are different than what previous studies of vegetarians have shown. There is a need for future research of the long-term health effects of being vegetarian.
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Van, der Merwe J. Debora. "Exposure to polyphenol-enriched rooibos (Aspalathus linearis) and honeybush (Cyclopia spp.) extracts : implications of metabolism for the oxidative status in rat liver." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71804.
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Thesis (PhD(FoodSc))--Stellenbosch University, 2012.ENGLISH ABSTRACT: Potential beneficial and/or adverse modulatory effects of polyphenol-enriched extracts of rooibos (Aspalathus linearis) and honeybush (Cyclopia spp.) on the antioxidant homeostasis in the liver were investigated. Phase II metabolism of aspalathin and mangiferin, the major polyphenols of rooibos and honeybush respectively, was assessed for potential glucuronidation and sulphation. Glucuronidation resulted in a loss of antioxidant activity for aspalathin and mangiferin in post-column HPLC-DAD-DPPH• and HPLC-DAD-ABTS•+ assays and also a decreased activity of iron chelating properties of mangiferin in the FRAP assay. Two independent studies for 28 and 90 days with polyphenol-enriched extracts (PEEs) of unfermented rooibos [Aspalathus linearis (PER)] and honeybush [Cyclopia. subternata (PECsub) and C. genistoides (PECgen)] in male Fischer rats were conducted to assess possible beneficial and/or adverse biological effects. PECgen was only included in the 28 day study. PEEs were characterised by in vitro antioxidant assays and HPLC analysis. The importance of detailed chemical characterization of rooibos and honeybush when investigating biological effects in vivo is clear as distinctive biological effects and major differences in compositions were evident. Biological parameters included were serum chemical parameters, activities of selected antioxidant enzymes, levels of glutathione and the modulation of expression of oxidative stress and antioxidant defense related genes in the liver. Sub-chronic (90 days) exposure of rats to PER and PECsub both adversely affected iron absorption significantly (P<0.05) and significantly (P<0.05) and markedly lowered levels of reduced glutathione (GSH) in the liver. The high levels of polyphenol intake were implicated and interaction with glutathione was postulated to occur via catechol o-quinone conjugations with GSH. This was also implicated in the significantly (P<0.05) increased activity of glutathione reductase (GR) following 28 days. These findings suggest that PEEs from rooibos and honeybush have the potential to alter the glutathione homeostasis, which could contribute to oxidative status in the liver. PECsub resulted in alterations in the liver biliary system which was manifested as significantly (P<0.05) increased serum total bilirubin (Tbili) and alkaline phosphatase (ALP), depending on the age of the rats, level of total polyphenols and duration of exposure. The expression of a number of oxidative stress and antioxidant defense related genes were differentially altered by the PEEs of rooibos and honeybush in rat liver and further indicated potential oxidative stress. Modulatory effects of PEEs on expression of 84 of these genes in rat liver were assessed with a quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) array and provided additional insights into the possible adverse and protective effects of rooibos and honeybush. Further investigation on total polyphenol dose levels and time of exposure in the application of PEEs of rooibos and honeybush as dietary supplements and functional foods is recommended and will also be of value in anticipated regulatory requirements for future substantiation of safety and efficacy.
AFRIKAANSE OPSOMMING: Die moontlike voordelige en/of nadelige modulerende effekte van polifenol-verrykde ekstrakte van rooibos (Aspalathus linearis) en heuningbos (Cyclopia spp.) op die antioksidant homeostasis in die lewer is ondersoek. Die fase II metaboliete van aspalatien en mangiferin, die hoof verbindings in rooibos en heuningbos onderskeidelik, is ondersoek t.o.v. glukuronidering en sulfonering. Glukuronidasie het gelei tot n verlies in antioksidant aktiwiteit van aspalatien en mangiferin soos bepaal in post-kolom HPLC-DAD-DPPH• en HPLC-DADABTS•+ toetse, asook verminderde interaksie met yster van mangiferin in die FRAP toets. Twee onafhanklike studies van 28 en 90 dae is onderneem met polifenol-verrykde ekstrakte (PVEs) van ongefermenteerde rooibos [Aspalathus linearis (PVR)] en heuningbos [Cyclopia. subternata (PVCsub) and C. genistoides (PVCgen)] in manlike Fisher rotte om die moontlike voordelige en/of nadelige biologiese effekte te ondersoek. PECgen was slegs in die 28 dae studie ingesluit. PVEs is gekarrakteriseer deur in vitro antioksidant en HPLC analises. Die belang van chemiese karaktirisering van rooibos en heuningbos tydens ondersoeke na biologiese aktiwiteit is duidelik aangesien verskeie en variërende biologiese aktiwiteite en verskille in die komposisie in die huidige studie gesien is. Die biologiese parameters wat ondersoek is om die effek van die PVEs te bepaal het serum kliniese parameters, aktiwiteit van geselekteerde ensieme, glutatioon en evaluering van die ekspressie van oksidatiewe en antioksidant verwante gene in die lewer, ingesluit. Sub-kroniese (90 dae) blootstelling van rotte aan PVR en PVCgen het yster absorpsie negatief beïnvloed. Die beduidende (P<0.05) verlaagde vlak van gereduseerde glutatioon in die lewer was toegeskryf aan die hoë vlakke van polifenole ingeneem tydens die studie en word moontlik veroorsaak deur n spesifieke katekol o-konjugasie van GSH. Hierdie interaksie was ook moontlik die oorsaak van n beduidende (P<0.05) toename in die aktiwiteit van glutatioon reduktase. Dié bevindinge het moontlike implikasies t.o.v die glutatioon homeostase en is n moontlike indikase dat PVEs van rooibos kan bydra tot oksidatiewe stres. PVCsub het veranderinge in die lewer gal-sisteem tot gevolg gehad aangesien daar n beduidende (P<0.05) verhoging in die serum totale bilirubin en alkalien fosfaat was. Hierdie veranderinge is beïnvloed deur die ouderdom, vlakke van die totale polifenole en die periode van blootstelling. Die uitdrukking van oksidatiewe en antioksidant verwante gene is op verskillende maniere beïnvloed deur die PVEs van rooibos en heuningbos in rot lewer and dien as n verdere indikasie van onderliggende oksidatiewe stres. Die modulerende effekte van PVEs op geenuitdrukking het gelei tot additionele insig aangaande die moontlike skadelike of beskermende eienskappe van PVEs vir gebruik as kruie produkte of dieet aanvullings. Die indikasies van moontlike oksidatiewe stres was duidelik van biologiese parameters en modulering van geenuitdrukking in die lewer, en vereis verdere ondersoek na die polifenool dosis en periode van toediening voordat PVEs van rooibos en heuningbos as funksionele voedsel produkte gebruik word. Hierdie ondersoek sowel as toekomstige ondersoeke in hierdie verband sal van waarde wees vir regulatoriese vereistes omtrent die veiligheid en effektiwiteit van rooibos en heuningbos kruie produkte.
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Vejnar, Sharon Trower. "Parents' perspectives and barriers regarding childhood overweight." CSUSB ScholarWorks, 2006. https://scholarworks.lib.csusb.edu/etd-project/3028.
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The purpose of this project was to explore parent perceptions about their children's weight and the perceived barriers to implementing healthy eating habits and patterns of physical activity for their children.
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De, Beer Dalene. "Antioxidant capacity of Pinotage wine as affected by viticultural and enological practices." Thesis, Stellenbosch: University of Stellenbosch, 2006. http://hdl.handle.net/10019.1/1300.
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Thesis (PhD (Food Science))--University of Stellenbosch, 2006.The aim of the study was to provide the South African wine industry with guidelines for the production of Pinotage wines with optimal total antioxidant capacity (TAC), while retaining sensory quality. The contribution of individual phenolic compounds to the wine TAC is important in this regard. The wine TAC was measured with the 2,2 -azino-di(3-ethylbenzo-thiazoline-sulphonic acid radical cation) (ABTS +) scavenging assay. The contributions of individual phenolic compounds to the wine TAC were calculated from their content in the wines and the Trolox equivalent antioxidant capacity (TEAC) of pure phenolic standards. The effects of climate region, vine structure, enological techniques (pre-fermentation maceration, juice/skin mixing, addition of commercial tannins, extended maceration) and maturation (oak barrels, alternative oak products, oxygenation) on the phenolic composition, TAC and sensory quality of Pinotage wines were also investigated. The TEAC values of quercetin-3-galactoside, isorhamnetin and peonidin-3-glucoside were reported for the first time. TEAC values observed for most compounds were much lower than those reported previously, although TEAC values for gallic acid, caftaric acid, caffeic acid and kaempferol were consistent with some previous reports. Caftaric acid and malvidin-3-glucoside were the largest contributors to the wine TAC. The contents of monomeric phenolic compounds and procyanidin B1, however, only explained a small amount (between 11 and 24%) of the wine TAC, with the remaining TAC attributed to oligomeric and polymeric phenolic compounds and other unknown compounds. Some synergy between different monomeric phenolic compounds was also demonstrated. All the viticultural and enological factors investigated affected the phenolic composition of Pinotage wines, while the wine TAC was only affected by some treatments. Changes in wine TAC could not always be explained by changes in phenolic composition as the contribution of oligomeric, polymeric and unknown compounds could not be assessed, but could play a large role. Differences in wine colour were also difficult to explain due to the large number of factors involved and the dark wine colour, which made objective measurements difficult. The concentration of vitisin A, an orange-red pyranoanthocyanin, was increased consistently as a result of prefermentation maceration treatments and affected the wine colour of oxygenated wines. Increased wine TAC was observed when cultivating Pinotage grapes on bush vines and in cooler climatic regions, compared to cultivation on trellised vines in warmer climatic regions. All the climatic regions and vine structure treatments, however, resulted in wines with good sensory quality. In terms of enological techniques, pumping-over, as opposed to punching-down and rotor treatments, is not recommended as a juice/skin mixing technique, due to reduced wine TAC, colour and sensory quality. Pre-fermentation maceration, addition of commercial tannin preparations, and oak maturation using traditional and alternative treatments, resulted in improved sensory quality, but with no change in wine TAC. However, optimisation of the tannin addition protocol may result in increased wine TAC if additions are made after fermentation or higher dosages are used. Oxygenation of Pinotage wine needs further investigation to optimise the protocol, as improvements to the wine colour and fullness were observed for some treatments, but loss of sensory quality and TAC were observed in most cases.
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Magwedere, Kudakwashe. "Veterinary public health aspects related with food-producing wildlife species in the domestic animal, human and environment interface." Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/80034.
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Thesis (PhD)--Stellenbosch University, 2013.ENGLISH ABSTRACT: The wildlife industry in Namibia continues to grow as the production and consumption of game meat increases. However, the health risks posed by the trade in wildlife and related by-products to livestock and humans have not been fully assessed. The main objective of this study was to investigate the potential health risks related to the increased consumption of game meat and relevant by-products by assessing the quality of game meat, as well as determine the role of game meat species in the transmission of zoonoses. The microbiological quality and safety of export game meat was assessed. No differences in the aerobic plate count (APC) were observed between the years (2009 and 2010), but the mean Enterobacteriaceae count was 1.33±0.69log10 cfu/cm2 compared to 2.93±1.50log10 cfu/cm2 between the years. Insignificant heterotrophic plate count (HPC) levels were detected in 9/23 field water samples, while faecal bacteria (coliforms, Clostridium perfringens and enterococci) were not isolated in all samples. Seven serogroups, with the exception of O26, were detected in exotic species. A white tailed deer sample had a serotype belonging to O45 which confirmed positive for stx1 gene. In springbok, 5/15 pools of faecal samples tested positive for the intimin gene. No Salmonella spp were isolated, and all E. coli isolates from the meat samples were negative for STEC virulence genes (i.e. stx1, stx2, eae and hlyA). A linear regression analysis was conducted on selected variables to identify the main predictors and their interactions affecting pH of meat 4 hours post-slaughter. In an increasing order of magnitude during winter time, the pH reached at 16-36hr post slaughter in springbok heart, liver, spleen, kidney and lungs was significantly higher than pH 6.0, while no significant differences were observed from the regulatory reference (pH 6.0) in the heart. There was a positive association between the pH of game meat 4hr post-slaughter, and liver congestion. The pH of game meat 4hr post slaughter, increased by 0.11 units per mL increase in liver congestion, and decreased by 0.04 units per minute increase in the shooting to bleeding interval, irrespective of the species. Worm eggs of strongylids, Strongyloides papillosus, Toxocara spp, Trichuris spp and coccidia were found in variable numbers in both springbok and gemsbok faeces, indicating a potential risk of transmission to other species in the ecosystem. On examination of carcasses, a novel parasite, Skjabinodera kuelzii, was identified and noted to be associated with inguinal fascia and renal fat, but the public health significance remains unclear. Nevertheless, S. kuelzii should be considered as of potential significance during routine game meat inspection. A total of 12 310 springbok were harvested from 26 commercial farms over a period of two years. Tissue samples (i.e. 60 livers, 41 kidneys and 52 hindquarter muscles) were collected from randomly selected healthy animals. The mean values (i.e. above the detection limit) of cadmium (Cd) and lead (Pb) were 0.10±0.05mg/kg and 1.04±0.21mg/kg in the liver, respectively; and 0.33±0.22mg/kg and 0.905±0.51mg/kg in the kidney of springbok, respectively. The levels of cadmium and lead in the hindquarter muscles were below the detection limit. Serum samples (n=1692) collected from sheep, goats and cattle from four presumably at-risk farms, and 900 springbok (Antidorcas marsupialis) serum samples collected from 29 mixed farming units, were screened for Brucella antibodies by using the Rose-Bengal test (RBT). Positive cases were confirmed by complement fixation test (CFT). To assess the prevalence of human brucellosis, 137 abattoir employees were tested for Brucella antibodies using the standard tube agglutination test (STAT), and the enzyme linked immunosorbent assay (ELISA). Cattle and sheep from all four farms were negative by RBT and CFT, but two of the four farms carried 26/42 and 12/285 seropositive goats, respectively. Post mortem examination of seropositive goats revealed no gross pathological lesions. Culture for brucellae from organs of seropositive animals was negative. None of the wildlife sera tested positive by either RBT or CFT. Occurrence of confirmed brucellosis in humans was linked to the consumption of unpasteurized goat milk, home-made goat cheese and coffee with raw milk and prior contact with goats. All abattoir employees (n=137) tested negative by STAT, but 3 were positive by ELISA. The three abattoir workers were clinically normal, and lacked historical connections with clinical cases. This study illustrates the importance of microbiological, parasitic and residue monitoring as critical components of a hazard analysis and critical control point based system for game meat. The study also provides the basis for increased integrated health research, surveillance and meat safety risk analysis.
AFRIKAANSE OPSOMMING: Die Namibiese wildbedryf raak toenemend groter soos die produksie en verbruik van wildsvleis toeneem. Die verwante gesondheidsrisiko’s wat die gebruik van wildsvleis en verwante produkte vir mens en dier inhou, is nog nie volledig geassesseer nie. Die doelwit van die studie was om ondersoek in te stel na die potensiële gesondheidsrisiko's wat wildsvleis en verwante neweprodukte vir mens en dier inhou deur middel van die assessering van vleisgehalte en die bepaling van die rol van die wildsvleis spesies in die oordrag van soönoses. Die mikrobiologiese gehalte en veiligheid van uitvoer wildsvleis was geassesseer. Geen verskille in die aerobiese plaat telling (APC) vir monsters versamel tydens 2009 en 2010 is aangeteken nie. Die gemiddelde Enterobacteriaceae telling was 1.33± 0.69log10 cfu/cm2 in vergelyking met 2.93±1.50log10 cfu/cm2 tussen die jare. Onbeduidende heterotrofe plaattelling (HPC) vlakke is waargeneem in 9/23 water monsters, terwyl fekale bakterieë (d.i. kolivorme, Clostridium perfringens en enterokokke) nie in enige van die monsters geïsoleerd is nie. Sewe serogroepe, met die uitsondering van O26, is aangeteken vir die eksotiese spesies. Monsters verky van ʼn white tailed deer is as positief vir 'n serotipe van O45 getoets, en die teenwoordigheid van die stx1 geen is bevestig. In springbok het 5/15 poele van fekale monsters positief getoets vir die intimien geen. Geen Salmonella spp is geïsoleer nie en alle E. coli geïsoleer in die vleismonsters was negatief vir die Stec virulensie geen (d.i. stx1, stx2, EAE en hlyA). ʼn Liniêre regressie-analise is op geselekteerde veranderlikes wat as die belangrikste indikators kan dien, en enige moontlike interaksie wat die pH van wildsvleis 4 uur na-slag kan beïnvloed, uitgevoer. In 'n toenemende orde van grootte gedurende die winter tyd, die pH teen 16-36hr na slagting in springbok hart, lewer, milt, niere en longe was aansienlik hoër as die pH 6.0, terwyl geen beduidende verskille waargeneem is wanneer dit met die regulasie verwysingswaarde van die hart (pH 6.0) vergelyk is nie. Daar was 'n positiewe assosiasie tussen die pH van wildsvleis 4 uur na-slag en mate van aansameling in die lewer. Die pH van wildsvleis 4 uur na-slag, het toegeneem met 0.11 eenhede per mL toename in lewer aansameling en afgeneem met 0.04 eenhede per minuut toename in die skiet tot uitbloei interval, ongeag die spesie. Wurmeiers van rondewurms, Strongyloides papillosus, Toxocara spp, Trichuris spp en koksidia het in verskillende ladings in die mis van beide springbok en gemsbok ontlasting, voorgekom. Dit dui op 'n potensiële risiko van oordrag na ander spesies in die ekosisteem. Die voorkoms van ʼn nuwe parasiet, Skjabinodera kuelzii, in wildskarkasse is aangeteken en was geassosieer met inguinale fascia en renale vet, maar die openbare gesondheidsrisiko bly onduidelik. Daar word aanbeveel dat dié parasiet as ʼn potensiële risiko faktor tydens roetine vleisinspeksies beskou moet word. ʼn Totaal van 12 310 springbokke is oor 'n tydperk van twee jaar van 26 kommersiële plase geoes. Weefselmonsters (d.i. 60 lewers, 41 niere en 52 agterkwart spiere) is ewekansig versamel van gesonde diere. Die gemiddelde waardes (d.i. hoër as die opsporingslimiet) van kadmium (Cd) en lood (Pb) was 0.10 ± 0.05mg/kg en 1.04 ± 0.21mg/kg in die lewer onderskeidelik en 0.33 ± 0.22mg/kg en 0.905 ± 0.51mg/kg in die niere van springbok, onderskeidelik. Die vlakke van kadmium en lood in die agterkwart spiere was laer as die opsporingslimiet. Serum monsters (n=1692) is van skape, bokke en beeste van vier vermoedelik hoë risiko plase en springbok (Antidorcas marsupialis, n=900) van 29 gemengde boerdery sisteme versamel en getoets vir die teenwoordigheid van Brucella teenliggaampies deur middel van die Rose-Bengal-toets (RBT). Positiewe gevalle is bevestig deur die komplement binding toets (CFT). Die voorkoms van menslike brusellose is bepaal deur 137 abattoir werknemers te toets vir Brucella teenliggaampies deur gebruik te maak van die standaard buis agglutinasie toets (STAT) en die ensiembinding immunosorberende toets (ELISA). Beeste en skape van die vier hoë risiko plase het negatief getoets met die RBT en CFT metodes, maar bokke van twee van die vier plase het seropositief getoets (26/42 en 12/285 onderskeidelik). Nadoodse ondersoek van seropositief bokke het geen patologiese letsels aangedui nie. Die kultuur van orgaanmonsters van seropositief diere vir Brucellae was negatief. Die monsters versamel van wild het negatief getoets deur middel van die RBT en CFT toets metodes. Die voorkoms van brusellose in mense in die studie was geassosieer met die gebruik van ongepasteuriseerde melk, tuisgemaakte bokmelkkaas en koffie met ongepasteuriseerde melk, asook direkte kontak met bokke. Alle abattoir werknemers (n=137) het negatief getoets met die STAT metode, maar drie werknemers het positief getoets met die ELISA metode. Die drie abattoir werkers was klinies normaal en het nie vorige kontak met bevestigde kliniese gevalle gehad nie. Hierdie studie bevestig die belang van mikrobiologiese, parasitiese en residu monitering as kritieke komponente van 'n gevaar-analise en kritiese kontrolepunt gebaseerde stelsel vir die produksie en verbruik van wildsvleis. Die studie verskaf ʼn basis vir toekomstige navorsing gefokus op ʼn geïntegreerde benadering van mens- en diergesondheid, monitering en vleis veiligheid risiko-analises.
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Fudge, Julie Lynn. "Explaining Adolescent Behavior Intention to Consume Fast Food Using the Theory of Planned Behavior." Diss., North Dakota State University, 2013. https://hdl.handle.net/10365/27202.
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This study tested the utility of the theory of planned behavior (TPB) to explain adolescent fast-food consumption among 349 high school adolescents. Subjective norms were further investigated to identify how parents and friends influenced adolescent fast-food consumption. Study participants completed a paper-based questionnaire measuring adolescent attitude, subjective norms for parents and friends, perceived behavioral control, and behavioral intention. Path analysis revealed that TPB explained adolescent fast-food behavioral intention to consume fast food. The model identified parent subjective norms had the strongest relationship with adolescent behavioral intention to consume fast food. Parent norms differed across age and grade in high school. Older adolescents perceived more approval for eating fast food than younger adolescents. Theoretical and practical implications are discussed.
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Seed, Barbara. "Food security in Public Health and other government programs in British Columbia, Canada : a policy analysis." Thesis, City University London, 2011. http://openaccess.city.ac.uk/1173/.
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Public Health has re-emerged as a driver of food security in British Columbia. Food security policy, programs and infrastructure have been integrated into the Public Health sector and other areas of government, including the adoption of food security as a Core Public Health program. This policy analysis of the integration merges findings from forty-eight key informant interviews conducted with government, Civil Society, and food supply representatives involved in the initiatives, along with relevant documents and participant/direct observations. Findings were analyzed according to “contextual”, “diagnostic”, “evaluative” and “strategic” categories from the Ritchie and Spencer framework for Applied Policy Research. While Civil Society was the driver for food security in British Columbia, Public Health was the driver for the integration of food security into the government. Public Health held most of the power, and often determined the agenda and the players involved. While many interviewees heralded the accomplishments of the incorporation of food security into Public Health, stakeholders also acknowledged the relative insignificance of the food security agenda in relation to other “weightier”, competing agendas. Conflict between stakeholders over approaches to food insecurity/hunger existed, and it was only weakly included in the agenda. Looking to consequences of the integration, food security increased in legitimacy within the Public Health sector over the research period. Interviewees described a clash of cultures between Public Health and Civil Society occurring partly as a result of Public Health’s limited food security mandate and inherent top down approach. Marginalization of the Civil Society voice at the provincial level was one of the negative consequences resulting from this integration. A social policy movement toward a new political paradigm - “regulatory pluralism” - calls for greater engagement of Civil Society, and for all sectors to work together toward common goals. This integration of food security into the government exemplifies an undertaking on the cutting edge in progress toward this shift. Recommendations for stakeholders in furthering food security within the government were identified. These include the development of food security policy alternatives for current government agendas in British Columbia, with a focus on health care funding, Aboriginal health and climate change.
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Little, David C. "An evaluation of strategies for production of Nile tilapia (Oreochromis Niloticus L.) fry suitable for hormonal treatment." Thesis, University of Stirling, 1989. http://hdl.handle.net/1893/2112.
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Intensive methods for the mass production of Oreochromis niloticus (Chitralada strain) seed using concrete tanks, hapas within earthen ponds and earthen ponds were investigated. On the basis of these trials, the productivity and economic performance of various production strategies for hormonally sex-reversed Oreochromis fry (MT fry) were then compared and modelled for adoption in Central and Northeast Thailand. Regular disturbance and harvesting of seed after a short period of spawning opportunity (5-10 days) was found to increase seed production in concrete spawning tanks. Exchange of female broodfish increased synchrony of breeding. A change in conditioning and spawning environment had no effect on seed yield from spawning tanks and hapas (area =12.57 m2and 40m2 respectively). Seed wet weight, seed clutch size and weight was greater in female fish spawned in tanks than hapas. Females conditioned in hapas however produced heavier seed clutches of larger absolute and relative size than tank conditioned fish. Records of tagged females indicated considerable differences in the frequency of spawning; in hapas the distribution was normal wheras in tanks it was skewed. The evidence suggests that hierarchy is important in the control of reproduction and exerts it's strongest effect in clear water, densely stocked tanks. Selective female broodfish exchange optimised seed yield per unit weight of broodfish and seed production was not improved by conditioning females for periods longer than 10 days. Male broodfish exchange did not significantly improve (P > 0.05) seed yields. Early nutrition of broodfish raised under different supplemental feeding regimes in fertilised earthen ponds had a significant effect on later spawning frequency in concrete tanks. However, this effect was confined to broodfish maintained at densities lower or higher than optimal for seed production. Broodfish stocked over a range of densities for extended periods (201 days) showed greater variability of seed production in hapa than tank production systems. This was mainly due to periods of poor water quality in hapas; when water quality was high seed production was significantly higher in hapas than tanks over a range of broodfish densities. The optimal density of broodfish for seed production was exceeded in tanks but not hapas. The relationship between seed production and broodfish density over time suggested that both stocking biomass and number have an effect on fry output. Density of broodfish showed an inverse relationship to clutch size in both tanks and hapas and synchrony of spawning in tanks. Production of swim-up fry in large earthen ponds (area=1740m2) was not significantly different (P>0.05) at 2 levels of harvest intensity. The use of small broodfish however produced double the yield of hormone treatable fry than a similar biomass of larger broodfish of the same cohort. A commercial scale incubation system was devised and evaluated in order to allow tank and hapa systems harvesting unhatched seed to be compared with the production of swim-up fry obtained from earthen ponds. Seed removed from mouthbrooding females was roughly staged and incubated in batches of similar development to give information on survival to swim-up fry. A simple incubation system was designed with a capacity for hatching >100,000 eggs/set. A mean survival of 75% of all harvested seed to swim-up fry was obtained over several trials. A trend to intensification (fry/mVday) from ponds to hapas to tanks was evident when yields of swim-up fry are compared. Productivity exceeded any in the published literature for comparable systems, largely because of the intensity of broodstock management and early and efficient harvest of seed. Broodfish productivities (fry/kg female/month) were also higher across the range of systems tested often by a factor of 1.5-3. The best strategies were selected over a range of total investment cost using dominance analysis. Economic analysis suggested that for a start-up operation in Central Thailand fry production in earthen ponds can give the best return on levels of investment of less than Baht 0.8 million. Substitution of techniques into current carp fry production operations in Northeast Thailand indicated that more intensive methods (production in tanks and hapas) are more attractive over a range of investment levels. The break-even price of MT fry after hormone treatment in nylon hapas was approximately half the cost of treatment in a recirculated water concrete tank system. The break-even price in Central Thailand was lower than the Northeast by a factor of around 1.5 but the break-even price for both areas was lower than the current price of untreated Oreochromis fry.
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Brink, Marelize. "Market and product assessment of probiotics and prebiotics and probiotic strains for commercial use." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/50011.
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Thesis (MSc Nutrition Science)--Stellenbosch University, 2004.ENGLISH ABSTRACT: Probiotics (live microbes) and prebiotics (non-digestible food-ingredients) are rapidly gaining interest worldwide as supplements and functional food ingredients but little South African information in this regard is available. Furthermore, the availability of South African produced probiotic concentrates for commercial use is also very limited. The aims of this study therefore were to complete a market and product assessment of probiotic and prebiotic containing products in South Africa and to evaluate probiotic strains for commercial use in South Africa. For the purposes of market and product assessment probiotic and/or prebiotic containing products manufactured in South Africa were identified. The scientific and legal correctness of health and content claims made on the labels of the products were assessed. An exploratory survey was conducted to determine the awareness of South African consumers of probiotics and prebiotics. For the evaluation of probiotic strains for potential commercial use in South Africa, a panel of twelve lactic acid bacteria (LAB) were screened for inhibitory activity against two porcine pathogens and indicator strains from the LMG-panel isolated from the faeces of patients diagnosed with AIDS. The five LAB with the best inhibitory activity were tested for growth in soymilk-base and for the effect of lyophilization on the inhibitory activity thereof. The effect of prebiotics on the growth and inhibitory activity of the strains was tested in vitro. A range of products containing probiotics and prebiotics available on the South African market was identified. Irregulatories concerning health claims on the labels were found, but content claims seemed to be less of a problem. The results also indicate that the proposed South African regulations for the labelling of probiotic and prebiotic containing products need to be revised to include the probiotic and prebiotic related health claims for which sufficient scientific evidence is available. The probiotic strains with potential for commercial use in South Africa that were identified, include Lactobacillus plantarum 423, Lactobacillus casei LHS, Lactobacillus salivarius 241, Lactobacillus curvatus DF38 and Pediococcus pentosaceus 34. These strains were grown successfully in soymilkbase and lyophilization did not have a negative effective on the inhibitory activity thereof. The growth and inhibitory activity of the five LAB were promoted when combined with 1% (w/v) Raftilose® Synergyl . It is concluded that although a variety of probiotic and prebiotic containing products are available on the South African market, the scientific and legislative correctness of especially health related claims is not satisfactory and that South African consumer awareness of these products is low. It is also concluded that a combination of at least three of the five identified LAB and 1% Raftilose® Synergy can be used by South African manufacturers for the production of probiotic and prebiotic containing supplements.
AFRIKAANSE OPSOMMING: Die belangstelling in probiotika (lewendige mikrobe) en prebiotika (onverteerbare voedselbestanddele) as supplemente en funksionele voedselbestanddele is besig om wêreldwyd toe te neem, alhoewel weinig Suid-Arikaanse inligting in hierdie verband beskikbaar is. Die beskikbaarheid van Suid-Afrikaans geproduseerde probiotika konsentrate vir kommersïele gebruik is ook baie beperk. Die doelwitte van hierdie studie was dus om 'n mark- en produkevaluering van probiotika- en prebiotika-bevattende produkte in Suid-Afrika uit te voer en om probiotiese stamme te evalueer vir uiteindelike kommersiële gebruik in Suid-Afrika. Vir die doel van die mark- en produkevaluering is probiotika- en prebiotika-bevattende produkte wat in Suid-Afrika vervaardig word geïdentifiseer. Die wetenskaplike en wetlike korrektheid van die gesondheids- en inhoudsaansprake op die etikette van die produkte is evalueer. 'n Markopname is uitgevoer om die bewustheid van Suid-Afrikaanse verbruikers van probiotika en prebiotika vas te stel. Vir die evaluering van probiotiese stamme vir potensïele kommersiële gebruik in Suid-Afrika is 'n paneel van twaalf melksuurbakteriëe getoets vir inhibitoriese aktiwiteit teen twee patogene geïsoleer uit varke asook teen indikator stamme van die LMG-paneel. Die vyf melksuurbakteriëe met die beste inhibitoriese aktiwiteit is getoets vir groei in sojamelk-basis en ook vir die effek van vriesdroging op die groei en inhibitoriese aktiwiteit van die stamme daarvan. Die effek van prebiotika op die groei en inhibitoriese aktiwiteit van die stamme is in vitro getoets. 'n Reeks van probiotika- en prebiotika-bevattende produkte wat beskikbaar is op die Suid-Afrikaanse mark, is geidentifiseer. Ongeruimdhede met die gesondheidsaansprake op die etikette is gevind, maar inhoudsaansprake was minder problematies. Die resultate dui ook daarop dat die voorgestelde Suid- Afrikaanse regulasies vir die etikettering van probiotika- en prebiotika-bevattende produkte hersien moet word om al die probiotika- en prebiotika-verwante gesondheidsaansprake waarvoor voldoende wetenskaplike bewyse beskikbaar is in te sluit. Die probiotiese stamme met potensiaal vir kommersiële gebruik in Suid-Afrika sluit die volgende in: Lactobacillus plantarum 423, Lactobacillus casei LHS, Lactobacillus salivarius 241, Lactobacillus curvatus DF 38 en Pediococcus pentosaceus 34. Hierdie stamme is suksesvol gekweek in sojamelk-basis en vriesdroging het nie' n negatiewe effek op die groei en inhibitoriese aktiwiteit daarvan gehad me. Die kombinasie van die vyf melksuurbakteriëe met 1% Raftilose® Synergy het die groei en inhibitoriese aktiwiteit daarvan bevorder. Die gevolgtrekking wat gemaak word is dat alhoewel 'n varrasie van probiotika- en prebiotikabevattende produkte beskikbaar is op die Suid-Afrikaanse mark, die wetenskaplike en wetlike korrektheid van spesifiek die gesondheids-verwante aansprake op die etikette daarvan nie bevredigend is nie en dat die bewustheid van die Suid-Afrikaanse verbruikers van hierdie produkte laag is. Die gevolgtrekking kan ook gemaak word dat 'n kombinasie van ten minste drie van die vyf geïdentifiseerde melksuurbakteriëe en 1% Raftilose® Synergy deur Suid-Afrikaanse vervaardigers gebruik kan word vir die vervaardiging van produkte wat probiotika en prebiotika bevat.
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Jimenez, Roxanne. "Effectiveness of Nonprofits on Factors That Influence the Social Aspects of Well-Being in Food Deserts." Case Western Reserve University School of Graduate Studies / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1620146299409908.
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單家驊 and Kar-wah Matthew Sin. "The study of residential roof tanks as a potential hazard." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31255292.
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Batina, Pierre. "Aspects metaboliques et toxicologiques de l'erythrocyte chez le rat traite aux nitrates et aux nitrites." Toulouse 3, 1988. http://www.theses.fr/1988TOU30196.
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Yake, Melissa L. "The effect of stress on eating practices among university faculty." Virtual Press, 2006. http://liblink.bsu.edu/uhtbin/catkey/1339600.
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The purpose of this study was to examine the relationship between comfort food preferences of college faculty when under normal (non-stressful) and stressful conditions. Thirty-four percent (n=63) of the participants were males and 66 percent (n=121) were females. Sixty-seven percent (n=123) experienced a change in appetite when stressed, with 69 percent (n=85) experiencing an increase and 31 percent (n=38) experiencing a decrease in appetite. Participants chose a significantly wider variety of foods when under stressful conditions for both sweet (p=<.001) and salty/crunchy food categories (p=.004). High restrained eaters chose significantly more types of sweet foods (p=.031) and beverages (p=.020) than low restrained eaters when comparing stressful and normal conditions. These findings suggest the majority of adults may experience changes in appetite with stress, specifically an increased appetite, and may choose more types of sweet and salty/crunchy foods. An individual's restraint level, gender, and age may also play a role in comfort food choice.Department of Family and Consumer Sciences