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Nielsen, Briant, and Oronhiett Rojas. "Helado Trencito." Tesis, Universidad de Chile, 2018. http://repositorio.uchile.cl/handle/2250/168508.
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Tesis para optar al grado de MAGÍSTER EN MARKETINGNielsen, Briant, [Parte I análisis situacional], Rojas, Oronhiett, [Parte II plan de marketing]
El chocolate Trencito es una reconocida barra de chocolate comercializada en Chile por la empresa multinacional Nestlé. El producto se caracteriza por su sabor dulce y textura suave, y quienes lo consumen, identifican la mezcla de sentimientos relacionados a la alegría y a la nostalgia en cada ocasión de consumo. Estos atributos lo hacen un chocolate muy popular entre la población chilena. Nestlé cuenta con una diversificada cartera de productos en diferentes categorías, entre ellas, la categoría de “chocolates” y la de “helados”. Los productos elaborados y distribuidos por esta empresa han logrado un alto nivel de penetración entre sus consumidores, por lo que resulta de gran interés realizar un estudio que abarque las posibilidades de extender un producto tan reconocido como el chocolate Trencito de Nestlé, a otra categoría, como lo es la del helado. Es así como, con el propósito de analizar los aspectos relativos al lanzamiento de una extensión de marca del producto Chocolate Trencito de Nestlé, en esta primera parte de la investigación, se hace relevante compilar los antecedentes necesarios que conformen un análisis situacional, el cual logre reflexionar acerca de los aspectos esenciales en el mercado y su entorno, y en donde se evidencie el potencial de los productos en la categoría de “helados” y “chocolates”, que combinados podrían resultar en la creación de un nuevo producto. En otras palabras, la elaboración de un producto que reúna los aspectos más queridos de un chocolate, y logre plasmarlos en ese nuevo formato: El Helado Trencito
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Cunningham, Phillip Scott. "Hello, Clothing." FIU Digital Commons, 2008. http://digitalcommons.fiu.edu/etd/2694.
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HELLO, CLOTHING is a collection of lyric poems about the connections between human beings, following an ekphrastic model that seeks truths about the world “second-hand”: through the language and images of other artists. A large section of the poems address the life and work of composer Morton Feldman, while many others explore the world of cinema or photography. The poems are particularly conscious of received forms. The collection takes to heart Harold Bloom’s assertion that “every poem is about another poem” and interprets this dictum as a celebration of formal structure. Whether through a traditional model such as the sonnet, sestina or villanelle; a stanzaic form derived from Elizabeth Bishop; or the re-writing of a single line by Denis Johnson, the book attempts to re-invent the work of its own inspiration, with the goal of discovering the inexhaustable pleasure of repetition.
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Bodin, Anders. "Helgo Zettervalls arkitektur." Doctoral thesis, KTH, Arkitekturens historia och teori, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-206814.
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Helgo Zettervall (1831–1907) was one of Sweden’s principal architects and design talents. He was professionally active for 40 years, from the late 1850s to the mid-1890s – a period of intensive building activity in Sweden. For the first twenty years of his career, he had his practice in Lund, and in the subsequent twenty years he lived in Stockholm as head of Sweden’s state authority for public buildings. Zettervall was very productive. His portfolio contains 281 projects, of which 160 were executed, ranging from large cathedral restorations with thousands of detailed drawings and large public buildings to porch extensions and smaller residences. This dissertation highlights Zettervall’s works by putting them in a context and by analysing their qualitative aspects. Carried out as a historiographical case study, the framework for the dissertation is the architectural monograph. The individual architectural projects are the core of the presentation, assembling and making archive material readily accessible, and the method is leaning towards that of an oeuvre complète raisonné. The method includes three distinct approaches: a factual accounting of documents and drawings, a context-setting frame story that provides the conditions, and an architectural analysis. Zettervall belonged to the first generation of architects who could receive a well-planned and broad training in Sweden. All of this training took place against a backdrop of what a specific assignment demanded in terms of responsiveness and understanding. Zettervall developed a special talent for utilizing new construction methods and materials. Each assignment was a challenge to investigate new design principles and new spatial ideas. The investigation shows that every project was unique for Zettervall, and that his various solutions depended on the specific situation, regarding site and program. Zettervall was a distinct pragmatic. Every assignment had its unique conditions, and thus had a unique solution.QC 20170511
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Schaden, Gerhard. "Say hello to markedness." Universität Potsdam, 2009. http://opus.kobv.de/ubp/volltexte/2009/3224/.
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In this paper, it will be shown that Bi-directional Optimality Theory (BOT) runs into problems of undergeneration when confronted with a certain class of partial-blocking phenomena. The empirical problem used to illustrate this is the cross-linguistic variation of one-step past-referring tenses. It will be argued that the well-known ‘present perfect puzzle’ is a sub-problem of it. The solution to the cross-linguistic variation of these tenses involves blocking of the marked tense. The relevant notion of ‘markedness’, while underivable synchronically, is argued to be linked to diachronic learning processes similar to those investigated by Benz (2006).
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Amorós, Gisela Analía, and Mariana Noguerol. "Producción de yogurt helado." Bachelor's thesis, Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria, 2014. http://bdigital.uncu.edu.ar/7913.
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El presente proyecto contiene el análisis y la evaluación técnico-económica para la producción de yogur helado. Se propone que el producto llegue al mercado en dos formas de presentación: envases de 250 g y envases de 500 g, cada uno de estos acompañados de los siguientes agregados: Chips de Chocolates, pasas de uva y crocantes, cereales y almendras, y microgalletitas de chocolate. El estudio realizado alcanza el nivel de prefactibilidad.
La calidad del producto estará determinada por los estándares establecidos en el Código Alimentario Argentino, aludiendo a lo legislado con respecto a yogur y helado, teniendo en cuenta que al ser un producto nuevo en el país no está contemplado su producción y comercialización; estos aspectos son fundamentales para que el mismo mantenga las características de un alimento inocuo, y cumpla con los requisitos de los clientes.Fil: Amorós, Gisela Analía. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria.
Fil: Noguerol, Mariana. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria.
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Blomberg, Sara. "Hela staden ska vara med! : Hela staden ska vara med!" Thesis, Örebro universitet, Institutionen för humaniora, utbildnings- och samhällsvetenskap, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-37078.
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Norstedt, Karin. "Hela barnet hela dagen : Om fritidshemspersonalens tillgång till specialpedagogiskt stöd." Thesis, Malmö universitet, Malmö högskola, Institutionen för skolutveckling och ledarskap (SOL), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-40084.
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Norstedt, Karin (2020). Hela barnet hela dagen, Om fritidshemspersonalens tillgång till specialpedagogiskt stöd. Specialpedagogprogrammet, Institutionen för skolutveckling och ledarskap, Lärande och samhälle, Malmö universitet, 90 hp. Förväntat kunskapsbidrag Det här arbetet väntades kunna bidra till att det forskas mer kring specialpedagogik på fritidshemmet. Att belysa i vilken omfattning en del personal i fritidshemmet upplevde stöd, ämnade studien ge en inblick i ämnet. Det här arbetet kan bidra med insikt och kunskap kring vikten av att det specialpedagogiska stödet till lärare ska fördelas efter behov även för personal på fritidshemmen. För specialpedagogen kan arbetet bidra till att belysa vikten av att fritidshemmet inkluderas i arbetet för barn i behov av särskilt stöd Syfte och frågeställningar Syftet med undersökningen är att ta reda på i vilken utsträckning personal i fritidshemmen har tillgång till specialpedagogiskt stöd samt hur detta stöd ser ut utifrån givna kategorier. Frågeställning Hur upplever verksamma inom fritidshemmet omfattning och innehåll i specialpedagogiskt stöd Vilket stöd ges och vilket stöd efterfrågas? Teori Den teoretiska utgångspunkten var Bronfenbrenners (1979) miljöekologiska systemteori som menar att delarna inte kan verka var för sig utan måste ses ur ett helhetsperspektiv. I sammanhanget är fritidshemmet en del av barnets hela skoltid dvs. helheten. Att bearbeta och analysera utifrån denna teori fyllde sitt syfte väl gällande att se delarna som en del av helheten. Teorin bearbetade materialet utifrån och in, där Bronfenbrenners (1979) ekologiska systemteori startar med makronivån som innefattade skollag och politik, värderingar och normer. Sedan följer exonivå som bland annat innefattar skolans organisation, sjukvård och andra organisationer som kan påverka individen indirekt. Mesonivån användes som ett sätt att se på samverkan mellan skola och hem och dess kunskap om varandra. Mikronivån är individen och den miljö och relationer den möter under dagen i skolans lokaler, dvs fritids-skola-fritids. Metod För att ta reda på hur många anställda (lärare i fritidshem, fritidspedagog och annan utbildning) i fritidshemmet som upplevde ett specialpedagogiskt stöd var kvantitativ enkätundersökning den metod som lämpade sig bäst. I enkäten användes slutna frågor för att kunna besvara frågeställningen. Detta sammanställdes i olika tabeller och figurer för att få en uppfattning kring stödet. Ett fritextsvar fanns under en del frågor och som resulterade i en djupare inblick i hur respondenterna upplevde stödet eller icke stödet. Resultat Resultatet visade att det finns en signifikant skillnad i upplevelsen av specialpedagogiskt stöd. Lite mer än 2/3 upplevde ett stöd som inte kan ses som tillräckligt (Ja, men inte i den utsträckning jag önskar eller Nej). Resultatet visade att det stödet som efterfrågas främst är handledning och stöttning kring lärmiljön. Detta gav indikationer på att det måste ske förändringar i systemet som skapar förutsättningar för specialpedagoger och verksamma i fritidshemmet att utveckla och vidmakthålla en verksamhet som lever upp till skollagen Specialpedagogiska implikationer Specialpedagogens uppgift är att se barnet som en helhet, dvs allt som på något sätt berör barnet, och det är helheten som ska ligga till grund för det stöd som ges. Det betyder att barnets kunskapsutveckling, sociala utveckling och hälsa är delar av helheten. Dessa delar existerar från tidig morgonfritids till lektionstid och till eftermiddagsfritids. Genom att handleda och använda fritidshemmens kunskap kring aspekter som direkt eller indirekt kan påverka elevens helhet, kan specialpedagogen vara med och förändra, stötta och anpassa även fritidshemmets verksamhet. Specialpedagogen måste ges förutsättningen till samverkan med alla delar och dessa förutsättningar är en del av huvudmännens uppdrag. Specialpedagogiken och det stöd specialpedagogen kan ge, bör innefatta fritidshemmet.
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Ytterberg, Adam. "Partikel hela vägen." Thesis, Konstfack, Institutionen för Konst (K), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-6074.
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i Uppsatsen försöker jag beskriva min senaste tid inom konstinstitutionen där jag brottats med vad det är jag vill påstå att min konst handlar om. jag demonstrerar med sakligt argumenterande och med en poetisk ton mitt sökande efter nyanser på min palett av skapelseprocesser för att komma närmare helhetsbilder.
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Billing, Mischa. "You had me at hello." Örebro universitet, Restaurang- och hotellhögskolan, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-29886.
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Setzer, Sidney. "Hello From Across the Past." ScholarWorks@UNO, 2010. http://scholarworks.uno.edu/td/1268.
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Elias, Tatiane de Oliveira. "Helio Oiticica : critica de arte." [s.n.], 2003. http://repositorio.unicamp.br/jspui/handle/REPOSIP/281550.
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Orientador : Nelson Alfredo AguilarDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciencias Humanas
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Resumo: Esta dissertação pretende apresentar as análises das obras de Oiticica cronologicamente. Serão apresentadas neste texto análises de obras inéditas como os desenhos de Oiticica para o filme da Cangaceira Eletrônica e o Projeto labirinto para Formigas. Também me concentrarei em eventos de Oiticica dos anos 70 que foram muito pouco estudados, e quase nada se pesquisou sobre eles. Farei uma análise histórica sobre o surgimento da Arte Concreta, da Escola de ULM, Primeira Bienal do Museu de Arte Moderna de São Paulo, a presença de Max Bill no Brasil. Relacionarei a obra de Oiticica com o contexto internacional. Pois, Oiticica estava inserido em um contexto das artes que rumavam para a desmaterialização, o fim das instituições museológicas e das artes tradicionais
Abstract: This dissertation intends to introduce the analysis of the Oiticica's works in a chronological order. It will be presented the analyses of the unpublished works, such as the Oiticica's draws for the film, "Cangaceira Eletrônica" and the "Projeto labirinto para Formigas". I will concentrate in the Oiticica's events of the 70's which were not weU studied - almost nothing was investigated about them. I will make a historical analysis about the rising of the "Arte Concreta" (Concrete Art) from the "Escola de ULM, Primeira Bienal do Museu de Arte Moderna de São Paulo", the presence of Max BiU in Brazil. I will relate the Oiticica's works with the international contexto Because Oiticica was inserted in a context of arts that go towards the desmaterialization, the end of the museum' s institutions and the traditional arts
Mestrado
Mestre em Filosofia
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Chaudhuri, Puspitapallab [UNESP]. "Espalhamento pósitron-hélio em energias intermediárias usando a aproximação Close-coupling." Universidade Estadual Paulista (UNESP), 1999. http://hdl.handle.net/11449/132771.
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Chaudhuri, Puspitapallab. "Espalhamento pósitron-hélio em energias intermediárias usando a aproximação "Close-coupling" /." São Paulo : [s.n.], 1999. http://hdl.handle.net/11449/132771.
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Dugonic, Vesna. "TAKK för hela förskolan." Thesis, Malmö universitet, Fakulteten för lärande och samhälle (LS), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-33437.
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AbstractDenna studie handlar om TAKK (Tecken som alternativ och kompletterande kommunikation), en metod som används i den svenska förskolan för att stimulera barns kommunikation och språkutveckling. Ambitionen med studien är att undersöka TAKK-metoden som komplement till det verbala språket vid kommunikation och språkinlärning. Mer specifikt är syftet med studien att undersöka hur pedagoger använder, motiverar och värderar TAKK i arbete och utbildning.Examensarbete startade med en fördjupning i Vygotskijs teorier i det sociokulturella perspektivet på lärande. Studien baseras på data insamlad via semistrukturerade intervjuer med sammanlagt fem pedagoger från två olika förskolor, från en mellanstor stad i västra Sverige. Resultaten visar att båda förskolorna använde sig av TAKK som redskap för att stödja barns kommunikation och språkutveckling. De intervjuade pedagogerna var positivt inställda till användningen av TAKK-metoden och ansåg att TAKK kunde skapa en inkluderande miljö, där barn blir delaktiga i kommunikationen och lär sig tillsammans i samspel med pedagogerna och med varandra. Resultaten visar att pedagogernas användning av TAKK påverkas av utbildning och stöd, att det behövs både tid att lära sig tecken och att alla i arbetslaget är med för att pedagogerna skulle använda sig av TAKK aktivt i sitt arbete. Slutligen framkom det att TAKK används för hela barngrupper och i kommunikationen med alla barn på förskolorna, oavsett ålder eller kulturell bakgrund.
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Lessa, Juliane Bellot Rolemberg. "O ensino na prancheta: da arquitetura escolar à docência de arquitetura e urbanismo, o legado de Hélio Duarte." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/16/16133/tde-27062017-163233/.
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A experiência profissional do carioca Helio de Queiroz Duarte foi rara em sua época, tendo sido um dos poucos arquitetos de sua geração a se dedicar à carreira acadêmica em paralelo e com igual vigor ao trabalho na prancheta. Valorizado pelos arquitetos, mas ainda pouco estudado, Duarte deixou como legado um novo paradigma de concepção e entendimento do uso e potencialidades da escola enquanto equipamento público. Ainda hoje tal herança rende frutos, notadamente nos projetos para os CEUs (Centros Educacionais Unificados). Sua atividade docente não foi menos impactante, afinal Duarte foi um dos fundadores da FAU/USP, um dos principais criadores do programa de pós-graduação dessa instituição (o primeiro do país) e também o responsável pela introdução do TGI (Trabalho de Graduação Interdisciplinar), hoje exigidos por todas as faculdades. O objetivo dessa pesquisa, portanto, é compreender como parte de sua produção se transformou em marco da arquitetura moderna nacional, balisa e referência para muitos arquitetos, em especialmente aqueles dedicados aos programas educacionais, tanto do ponto de vista espacial quanto pedagógico ainda hoje. Pretende-se também compreender a influência de sua atuação docente e acadêmica na conformação e sistematização do ensino de graduação e pós graduação de arquitetura e urbanismo no país. Bem como, verificar como o conjunto de suas atividades colaborou na consolidação e reconhecimento da profissão de arquiteto e urbanista em São Paulo.The professional experience of Helio de Queiroz Duarte from Rio de Janeiro was rare in his time, he was one of the few architects of his generation to dedicate the same energy and intensity to both the academic career and the job on the drawing board. Appreciated by contemporary architects, but still little studied, Duarte left us as legacy a new paradigm of concept and understanding of the uses and potentiality of schools as public equipment. This heritage still bears fruit nowadays, as can be seen in the projects for the CEUs (Centers for Unified Education). His academic activity was just as impactful, after all, Duarte was one of the founders of the Faculty of Architecture and Urbanism at the University of São Paulo, one of the main creators of the first postgraduate program in the country for this same institucion, as well as for the introduction of the TGI (Final Graduation Interdisciplinary Work), required today by all colleges. The aim of this research is to understand how part of his prodution became a reference for Brazilian modern architecture, which distinguishes and references the production of mainly educational programs, both from the spatial and pedagogical point of view until today. This work intends to measure the influence of his academic activity in the conformation and systematization of the undergraduate and postgraduate education of architecture and urbanism in Brazil. At the same time, it seeks to verify how the complex of Helio Duarte\'s activities collaborated in the consolidation and recognition of the architect and urbanist profession in São Paulo.
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Hillring, Björn. ""De ser hela barnet, hela dagen" : Fritidspedagogers uppdrag och ansvarsområden sett med rektorers glasögon." Thesis, Umeå universitet, Institutionen för tillämpad utbildningsvetenskap, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-121124.
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Syftet med denna studie var att erhålla kunskap om hur rektorer i grundskolan uppfattar fritidspedagogers ansvarsområden inom skolans verksamhet och det uppdrag som anges i styrdokumenten vad gäller barns och elevers tid i skola och fritidshem. Undersökningen utfördes genom att åtta rektorer intervjuades via semistrukturerade kvalitativa intervjuer med fenomengrafiska influenser i analysen. Studien visade på ett uppdrag som utifrån styrdokument bottnar i att se barnen och den grupp de är i och att arbeta med dem i olika sociala sammanhang. Typiska ansvarsområden som anges är delaktighet i samverkan med skolan på olika sätt och att föra dokumentation av verksamheten så den går att utvärdera och utveckla. I intervjuerna framkom att rektorerna ser fritidspedagogerna som kunniga inom området med grupporienterade processer och att de är duktiga på att komplettera skolan på olika sätt samt utgå från uppdraget som finns i styrdokumenten. Andra slutsatser var att det sytematiska kvalitetsarbetet är utmanande att hitta en bra systematik till och dokumentation avverksamheten kan bli bättre. Ytterligare en slutsats var att samverkan kan vara av olika karaktär, ofta är det som resurs fritidspedagogen samverkar.
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Tabet-Helal, Sana Zouleykha. "Impacts moléculaire et cellulaire de mutations de la partie N-terminale de la stathmine." Thesis, Evry-Val d'Essonne, 2014. http://www.theses.fr/2014EVRY0010/document.
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Les microtubules (MTs) sont des éléments majeurs du cytosquelette, ils participent à un ensemble de fonctions cellulaires d’importance comme la migration, le trafic des vésicules et des organelles ainsi qu’{ la division cellulaire au cours de laquelle ils forment le fuseau mitotique, permettant une ségrégation correcte des chromosomes. Ce sont des polymères cylindriques composés de protofilaments parallèles faits d’hétérodimères de tubulines α/β. Pour exercer ces fonctions, les MTs nécessitent une dynamique d’assemblage et de désassemblage régulée.La stathmine est une phosphoprotéine cytosolique de 17 kDa qui séquestre la tubuline dans un complexe non polymérisable formé de deux hétérodimères de tubuline par molécule de stathmine (complexe T2S). Les autres protéines de la famille stathmine (SCG10, SCLIP, RB3 et ses deux variants d’épissage RB3' et RB3″) forment aussi des complexes de séquestration appelés T2-SLD (Stathmin Like Domain) ayant des stabilités variables.En s’appuyant sur ces observations, des travaux ont montré qu’une chimère SN-CR constituée de la partie N-terminale de la stathmine et de la partie C-terminale du SLD de la protéine RB3 forme un complexe T2S remarquablement stable avec la tubuline (Jourdain et al., 2004). En parallèle à ces travaux, Clément et collaborateurs ont découvert que de courts peptides issus de la région N-terminale des SLD peuvent à eux seuls inhiber la polymérisation de la tubuline. Le peptide le plus efficace est le peptide I19L issu de la stathmine (peptide I19L (IQVKELEKRASGQAFELIL)). Il correspond à la région repliée en «β-hairpin» observée dans les cristaux T2S-SLD formés avec le domaine RB3-SLD (Clement et al., 2005). Pour augmenter l'efficacité de l’interaction entre les peptides issus de la région N-terminale des SLD et la tubuline, différentes mutations ont été introduites au niveau du peptide I19L. Les expériences de polymérisation in vitro de tubuline en présence d’une série de mutants de peptide I19L montrent que les peptides I19L-K4R et I19L-K4R-A10R sont les plus efficaces pour l’inhibition de l’assemblage de la tubuline.L’objectif de mes travaux { été d’introduire ces mutations au niveau de la partie N-terminale de la protéine stathmine et de la chimère NS-CR, afin d’analyser leurs efficacités sur l’inhibition de l’assemblage des MTs et la dynamique des MTs dans des cellules en culture de type HeLa, et de voir leur impact sur le cycle cellulaire.Mes résultats montrent que la stathmine mutant 2 (possédant les mutations K9R-A15R homologues aux mutations K4R-A10R du peptide I19L) induit une dépolymérisation des MTs dans des cellules en mitose par formation de fagots de MTs, réduit la dynamique de MTs et induit une cytotoxicité. L’ensemble des résultats suggère que nous pouvons améliorer la stabilité de liaison de la stathmine avec la tubuline par l’introduction de ces mutations, ce qui aurait in vitro un impact dans certains aspects cellulaires comme la prolifération cellulaire et la dynamique des MTs. Cela pourrait conduire à développer de nouvelles stratégies anticancéreuses visant à perturber le fuseau mitotiqueMicrotubules (MTs) are major constituents of the cytoskeleton and are involved in many cellular processes such as the determination of cell architecture, the formation of the mitotic spindle and intracellular trafficking. These tubular structures, composed by tubulin α/β heterodimers assemble and disassemble with a finely regulated dynamics.Stathmin is a cytosolic phosphoprotein that sequesters tubulin in a non polymerizable complex consisting of two tubulin heterodimers per stathmin molecule (T2S complex).The other stathmin family proteins (SCG10, SCLIP, RB3 and its two splice variants RB3 'and RB3 ") can also bind two tubulin heterodimers through their SLD (Stathmin Like Domain), but the different tubulin/SLD complexes display varying stabilities.Based on these observations, current works showed that one chimera protein consisting of the N-terminal domain of stathmin (NS) and of the C-terminal domain of RB3 (CR) forms a T2-SLD complex remarkably stable with tubulin (NS-CR chimera). Molecular simulation experiments in silico suggest that we can increase the affinity of the NS-CR chimera by introducing mutations in the NS subdomain (Jourdain et al., 2004).In parallel to this work, Clement and colleagues found that short peptides derived from the N-terminal region of SLD alone can inhibit tubulin polymerization. Among the N-terminal part of SLD, the most effective peptide is the peptide I19L (IQVKELEKRASGQAFELIL) derived from stathmin. It corresponds to region folded into a "β-hairpin" structure observed in the T2S crystals (Clement et al., 2005). To increase the efficiency of this interaction, different mutations were introduced at the level of the I19L peptide from the stathmin N-terminal domain and tested in vitro. The results showed that the peptides I19L-K4R and I19L-K4R-A10R are the most effective to in inhibit the assembly of MTs.In this work, we introduce these mutations in the N-terminal domains of stathmin and in the NS-CR chimera with the aim of analyzing their efficiencies on MTs disassembly in cancer cell cultures like HeLa, and the impact of the mutation on MTs dynamic and cell cycle.Results from overexpression experiments of WT and stathmin mutant 2 containing the double mutation (K9R-A15R similar to the mutations K4R-A10R for the I19L peptide), suggest that this mutant significantly induces depolymerization of mitotic MTs. In its less phosphorylated state on serine 16; this mutation also reduces MTs dynamics and induces cytotoxicity.We thus suggest that we can ameliorate the binding and efficiency of the N-terminal stathmin part by including this double mutation. This variation has an impact in vitro and in some cellular aspects such as cell proliferation and MTs dynamics. These findings may lead to a targeted therapy for this type of cancer
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Bergh, Nestlog Ewa. "Hela svenskan : Integrering inom svenskämnet." Thesis, Växjö University, School of Humanities, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:vxu:diva-1794.
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Syftet med denna uppsats är att undersöka en funktionell svenskundervisning där undervisning om språkliga strukturer integreras i undervisningen kring eget skrivande och analys av egna och andras texter. Fokus ligger på undervisningens relation till kontextuella aspekter av verksamheten, såsom sammanhang som har att göra med praxis i klassrummet, den enskilda skolan och yttre ramar på ett samhälleligt plan. Centrala begrepp är dialogicitet, processer, stödstrukturer, undervisning rörande språkliga strukturer, integrering, erfarenhetspedagogisk ämnessyn och text som socialt beteende. Såväl min analysapparat som den studerade undervisningen har inspirerats av ett sociokulturellt perspektiv på lärande, särskilt nydialogiska och genrepedagogiska teorier.
Studien följer fyra undervisningsförlopp i grundskolans senare år och i gymnasieskolan genom observationer, samtal i fokusgrupper samt intervjuer med lärare och elever.
Resultatet visar att den genomförda undervisningen värderas högt av lärare och elever, och lärarna menar att alla kategorier av elever tjänar på denna typ av integrerad undervisning. Den upplevs som effektiv och engagerande. Vad gäller de centrala begreppen, som nämnts ovan, kan man konstatera att det verkar ha funnits en strävan att implementera dessa i den studerade undervisningen, vilket också har skett i olika grad. En ytlig iscensättning av dessa begrepp i praktiken innebär dock knappast en undervisning som kan anses gestalta forskares teorier kring dessa begrepp. Ett flertal kontextuella aspekter på olika nivåer har identifierats som betydelsefulla i sammanhanget, som exempelvis läromedlens roll; lärarnas kunskaper och syn på lärande, på svenskämnet och på texter; lärarnas behov av forum för diskussioner och reflektion kring svenskundervisningen.
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Lindskog, Ann-Sofie, and Ida Ringdahl. "Ovisshet upptar människans hela sfär." Thesis, Kristianstad University College, School of Health and Society, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-5097.
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Bakgrund: Att inte veta hur saker och ting förhåller sig, är att sväva i ovisshet. Att leva i ovisshet och att inte veta hur ens framtid kommer att bli kan skapa funderingar som kan leda till olika reaktioner. Sådana funderingar och reaktioner blir ofta synliga i berättelser om det inträffade. En berättelse är en skildring av ett händelseförlopp muntligt eller skriftligt av verkliga eller påhittade händelser. Genom berättandet fångas helheten upp och återskapar upplevelser och känslor. Syfte: Syftet med studien var att få en ökad förståelse för fenomenet ovisshet. Metod: Studien genomfördes som en empirisk studie där studenters livsvärldsberättelser om fenomenet ovisshet utgjorde grunden för resultatet. Materialet har analyserats med kvalitativ innehållsanalys. Resultatet: Resultatet presenterades utefter tre frågor: 1 Vad innebär ovisshet? Tankar om framtiden och att inte ha makt att kunna påverka, 2 När uppkommer ovisshet? När det vardagliga bryts och 3 Vad gör ovisshet med människan? Känslor här och nu, tankar som svävar iväg. Diskussion: En ökad förståelse för vad ovisshet är underlättar för sjuksköterskan i hennes arbete att vårda och förstå den enskilde patientens livsvärld.
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Bootman, Martin D. "Calcium spiking in HeLa cells." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281898.
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Malmborg, Nordgren Camilla. "Viktigt på riktigt, hela dagen." Thesis, Malmö universitet, Fakulteten för lärande och samhälle (LS), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-33712.
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Syftet med denna studie har varit att undersöka barns inflytande och delaktighet vid en fri skapandesituation i ateljé och hur barn och pedagoger förhåller sig till varandra. Resultatet visar att pedagogerna har svårt att dela upp begreppen inflytande och delaktighet och använder dem synonymt. Pedagogerna är medvetna om att makt utövas men omedvetna om maktens olika nyanser. Studiens resultat visar på at det finns begränsningar i barns reella inflytande och delaktighet i den fria skapandesituationen där pedagogernas förhållningssätt speglar olika nyanser av maktutövningar.
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Uriondo, Boudri Heydy Wendy. "Estudios sobre la exportación de la proteína quinasa dependiente de cAMP en células humanas en cultivo." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2007. https://hdl.handle.net/20.500.12672/2515.
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La holoenzima proteína quinasa A (PKA) es un tetrámero que consiste en dos subunidades catalíticas y dos subunidades reguladoras, se encuentra en forma ubicua fosforilando un gran número de proteínas celulares y participa en la regulación de vías metabólicas y otros procesos (transporte de membrana, motilidad celular, expresión génica, apoptosis, aprendizaje y memoria).
Las proteínas quinasas no solamente se encuentran en el interior de las células, sino además se observa la "exportación" hacia la superficie de la cara externa de la membrana plasmática de las células (Redegeld, F.A et al., 1999). Este fenómeno de exportación de las proteína quinasas hacia la superficie de la cara externa de las células en cultivo se denomina "ectoquinasas."
El objetivo del presente trabajo es estudiar la exportación de la proteína quinasa dependiente del AMPc sobrexpresada en células humanas en cultivo a nivel intracelular o endógeno, a nivel ectópico o en la membrana plasmática, y libre en el líquido extracelular o proteína exógeno.
En la presente Tesis se utilizó dos líneas celulares llamadas HEK293T y HELA, realizándose la técnica de transfección por lipofectamina para introducir en estas células la proteína PKA, específicamente el vector (pcDNA3-HA) con el inserto que contiene el gen de la subunidad catalítica con el epítope anti-HA.Tesis
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Olén, Benkt. "Rymlighetens evangelium : Helgo Zettervall som kyrkoarkitekt i Lunds stift i ljuset av samtida kyrkoliv /." Lund : Universitet, 1987. http://catalogue.bnf.fr/ark:/12148/cb413118583.
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Doctoral dissertation--Lund, 1987.Bibliogr. p. 163-174. Résumé en anglais sous le titre : "The gospel of spaciousness : Helgo Zettervall as a church architect in the diocese of Lund in the light of contemporary church life"
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Kuszlewicz, James Stevenson. "Buoyancy-driven oscillations in helio- and asteroseismology." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7658/.
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This thesis focuses on the application of asteroseismology to red giants observed with Kepler alongside searching for solar g-modes using the Birmingham Solar Oscillations Network (BiSON). In the case of the Sun, solar gravity modes are highly sought after because they can shed light on the inner rotation profile of the Sun. This thesis contains work showing how the low frequency regime of BiSON data has been cleaned enabling the search to be made in BiSON data without instrumental artefacts. Moving onwards along the stars evolution, thanks to space mission such as Kepler and CoRoT tens of thousands of red giant stars have been observed allowing huge ensemble investigations. The ability to use high-quality, long datasets as constraints to shorter and noiser datasets has been investigated through fitting the background power of 6000 Kepler red giants. Red giants also offer the opportunity to study the inclination angle distribution of stars to confirm that the distribution conforms to the expected isotropy used in many simulations. This can be extended to inferring the obliquity through asteroseismology, as applied to a red-giant, M-dwarf eclipsing binary. This offering a means to probe obliquity distributions in in a different regime to that using traditional spectroscopic techniques.
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Arlt, Wulf. "“Helas” / “Las” in Liedanfängen des 15. Jahrhunderts." Bärenreiter Verlag, 1998. https://slub.qucosa.de/id/qucosa%3A37032.
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Souza, Aryane Tofanello de [UNESP]. "Síntese e caracterização de nanopartículas magnéticas de óxido de ferro para aplicações biomédicas – um estudo citotóxico em linhagem celular de carcinoma cervical humano (células HeLa)." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/92023.
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000680523.pdf: 1511132 bytes, checksum: c20e5a6a65edf2cba90d4de2b5073a08 (MD5)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Nanopartículas magnéticas (NPMs) têm sido alvo de inúmeras investigações por seu grande potencial de aplicação nos mais diferentes campos tecnológicos. Dentre tantos, elas destacam-se na área biomédica, seja no diagnóstico ou tratamento de diversas doenças. Neste trabalho foram sintetizadas nanopartículas magnéticas (NPMs) de óxido de ferro (magnetita) pelo método de coprecipitação de íons Fe2+ e Fe3+ em meio alcalino. O objetivo central foi estudar as características morfológicas, estruturais, magnéticas e o comportamento biológico desses compostos em células cancerígenas, visando futuras aplicações biomédicas. Inicialmente as nanopartículas magnéticas foram avaliadas em função dos parâmetros físico-químicos que influenciam diretamente as características finais do produto (pH, molaridade, temperatura e tipo de base) para se observar as melhores condições de síntese e a influência de cada um nas características do produto. As nanopartículas foram caracterizadas por difratometria de raios-X, microscopia eletrônica de varredura, potencial zeta e magnetometria. Desse primeiro estudo concluiu-se que a coprecipitação produz partículas com polidispersão de tamanhos alta e que os parâmetros de síntese influenciam drasticamente as propriedades dos materiais, no entanto, todas as amostras exibiam características magnéticas. Depois de estabelecida esta etapa, as NPMs foram submetidas à transfecção em cultura celular de carcinoma cervical humano (células HeLa) e a testes biológicos como coloração com Azul da Prússia e hematoxilina-eosina, ensaio de MTT e ensaio de apoptose para averiguação da citotoxidade. A principal observação vinda desses resultados foi que as nanopartículas magnéticas sintetizadas, salvo algumas adaptações de síntese...
Magnetic nanoparticles (MNPs) have been the subject of numerous investigations because of its great potential application in many different fields of technology. Among many, they stand out in the biomedical area, either in diagnosis or treatment of various diseases. In this work were synthesized magnetic nanoparticles (MNPs) of iron oxide (magnetite) by the coprecipitation method of Fe2+ and Fe3+ in an alkaline medium. The main objective was to study the morphological, structural, magnetic and biological behavior of these compounds in cancer cells, in order to future biomedical applications. Initially the magnetic nanoparticles were evaluated against the physical and chemical parameters that directly influence the final characteristics of product (pH, molarity, temperature and type of base) to observe the best synthesis conditions and influence of each characteristics. The nanoparticles were characterized by X-ray diffraction, sccaning electron microscopy, zeta potential and magnetometry. In this first study showed that coprecipitation produces particles with high polydispersity of sizes and that the synthesis parameters dramatically influence the properties of materials, however all samples exhibited magnetic characteristics. After you make this step, the MNP were subjected to transfection in cell culture of human cervical carcinoma (HeLa) and biological tests such as staining with Prussian blue and hematoxylin-eosin, MTT assay and apoptosis assay to investigate cytotoxicity. The main observation was that these results coming magnetic nanoparticles synthesized, except for some adjustments in short, constitute a class of nanocomposites with enormous potential for therapeutic and / or diagnosis. The work brings together information ranging from the synthesis of nanoparticles to their behavior inside the cells, emphasizing the best conditions for each procedure
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Oliveira, Ruth Medeiros de. "Avalia??o das atividades antioxidante, anticoagulante e antiproliferativa de extratos aquosos de marsdenia megalantha." Universidade Federal do Rio Grande do Norte, 2011. http://repositorio.ufrn.br:8080/jspui/handle/123456789/12576.
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Previous issue date: 2011-03-21Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior
The species of the genus Marsdenia, Apocynaceae, are widely used in folk medicine of several countries. In Brazil is found several species belonging to this genus. The in vitro antioxidant, anticoagulant and antiproliferative activities were evaluated to aqueous extracts of stalk, leaf and root of Marsdenia megalantha. In the total antioxidant capacity assay (expressed as ascorbic acid equivalents) the stalk extract showed 76.0 mg/g, while leaf and root extracts 141.3 mg/g and 57.0 mg/g, respectively. The stalk and leaf extracts showed chelating activity around 40% at 1.5 mg/mL, while root extract, at the same concentration showed, 17%. Only the leaf extract showed a significant ability in superoxide scavenging (80% at 0.8 mg/mL). Any extract was able in scavenge hydroxyl, as well anticoagulant activity. The antiproliferative activity of the extracts was evaluated against HeLa tumor cell line. The extracts inhibited in a dose-dependent manner the cell growth. However, the leaf extract showed 80% of inhibition at 1.0 mg/mL, while stalk and root extracts inhibited 63% and 30%, respectively. To assess the mechanism of cell death caused by the leaf extract in HeLa, was performed flow cytometry and western blot. The results show that leaf extract induces cell death by apoptosis through an activation caspase-independent pathway. These data indicate that stalk and leaf extracts obtained have potential to be used as antioxidants and anticancer drugs
As esp?cies do g?nero Marsdenia, Apocynaceae, s?o bastante utilizadas na medicina popular de v?rios pa?ses. No Brasil s?o encontradas v?rias esp?cies pertencentes a esse g?nero. As atividades antioxidante, anticoagulante e antiproliferativa foram avaliadas para os extratos de caule, folha e raiz de Marsdenia megalantha. Na capacidade antioxidante total (expressa como equivalente de ?cido asc?rbico), o extrato do caule apresentou 76,0 mg/g, enquanto os extratos da folha e raiz apresentaram, respectivamente, 14,3 mg/g e 57,0 mg/g. Os extratos do caule e da folha mostraram habilidade quelante em torno de 40% na concentra??o de 1,5 mg/mL, enquanto o extrato da raiz, na mesma concentra??o, apresentou 17%. Apenas o extrato da folha apresentou uma capacidade significante em sequestrar radicais super?xidos (80% em 0,8 mg/mL). Nenhum extrato mostrou capacidade em seq?estrar radicais hidroxila, bem como atividade anticoagulante. A atividade antiproliferativa dos extratos foi avaliada contra a linhagem tumoral HeLa. Os extratos inibiram, de forma dose-dependente, o crescimento celular. Entretanto, o extrato da folha foi capaz de inibir em 80% a prolifera??o celular na concentra??o de 1,0 mg/mL, enquanto os extratos de caule e raiz inibiram 63% e 30%, respectivamente. Para avaliar o mecanismo de morte celular causada pelo extrato da folha nas c?lulas HeLa, foi realizada citometria de fluxo e western blot. Os resultados mostraram que o extrato da folha induz morte celular por apoptose atrav?s de uma via de ativa??o independente de caspase. Estes resultados indicam que os extratos de caule e folha obtidos t?m potencial para serem futuramente utilizados como f?rmacos antioxidantes e antic?ncer
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Souza, Aryane Tofanello de. "Síntese e caracterização de nanopartículas magnéticas de óxido de ferro para aplicações biomédicas - um estudo citotóxico em linhagem celular de carcinoma cervical humano (células HeLa) /." São José do Rio Preto : [s.n.], 2011. http://hdl.handle.net/11449/92023.
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Orientador: José Geraldo NeryBanca: Paula Rahal
Banca: Maria Cristina Nonato Costa
Resumo: Nanopartículas magnéticas (NPMs) têm sido alvo de inúmeras investigações por seu grande potencial de aplicação nos mais diferentes campos tecnológicos. Dentre tantos, elas destacam-se na área biomédica, seja no diagnóstico ou tratamento de diversas doenças. Neste trabalho foram sintetizadas nanopartículas magnéticas (NPMs) de óxido de ferro (magnetita) pelo método de coprecipitação de íons Fe2+ e Fe3+ em meio alcalino. O objetivo central foi estudar as características morfológicas, estruturais, magnéticas e o comportamento biológico desses compostos em células cancerígenas, visando futuras aplicações biomédicas. Inicialmente as nanopartículas magnéticas foram avaliadas em função dos parâmetros físico-químicos que influenciam diretamente as características finais do produto (pH, molaridade, temperatura e tipo de base) para se observar as melhores condições de síntese e a influência de cada um nas características do produto. As nanopartículas foram caracterizadas por difratometria de raios-X, microscopia eletrônica de varredura, potencial zeta e magnetometria. Desse primeiro estudo concluiu-se que a coprecipitação produz partículas com polidispersão de tamanhos alta e que os parâmetros de síntese influenciam drasticamente as propriedades dos materiais, no entanto, todas as amostras exibiam características magnéticas. Depois de estabelecida esta etapa, as NPMs foram submetidas à transfecção em cultura celular de carcinoma cervical humano (células HeLa) e a testes biológicos como coloração com Azul da Prússia e hematoxilina-eosina, ensaio de MTT e ensaio de apoptose para averiguação da citotoxidade. A principal observação vinda desses resultados foi que as nanopartículas magnéticas sintetizadas, salvo algumas adaptações de síntese... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Magnetic nanoparticles (MNPs) have been the subject of numerous investigations because of its great potential application in many different fields of technology. Among many, they stand out in the biomedical area, either in diagnosis or treatment of various diseases. In this work were synthesized magnetic nanoparticles (MNPs) of iron oxide (magnetite) by the coprecipitation method of Fe2+ and Fe3+ in an alkaline medium. The main objective was to study the morphological, structural, magnetic and biological behavior of these compounds in cancer cells, in order to future biomedical applications. Initially the magnetic nanoparticles were evaluated against the physical and chemical parameters that directly influence the final characteristics of product (pH, molarity, temperature and type of base) to observe the best synthesis conditions and influence of each characteristics. The nanoparticles were characterized by X-ray diffraction, sccaning electron microscopy, zeta potential and magnetometry. In this first study showed that coprecipitation produces particles with high polydispersity of sizes and that the synthesis parameters dramatically influence the properties of materials, however all samples exhibited magnetic characteristics. After you make this step, the MNP were subjected to transfection in cell culture of human cervical carcinoma (HeLa) and biological tests such as staining with Prussian blue and hematoxylin-eosin, MTT assay and apoptosis assay to investigate cytotoxicity. The main observation was that these results coming magnetic nanoparticles synthesized, except for some adjustments in short, constitute a class of nanocomposites with enormous potential for therapeutic and / or diagnosis. The work brings together information ranging from the synthesis of nanoparticles to their behavior inside the cells, emphasizing the best conditions for each procedure
Mestre
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Flynn, Patrick G. "Activation of Non-Muscle Myosin IIB Helps Mediate TNF-Alpha Cell Death Signaling." Scholarly Repository, 2010. http://scholarlyrepository.miami.edu/oa_dissertations/369.
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TNF-alpha can stimulate a variety of kinases with the ability to activate non-muscle myosin II. As a result, increases in actin filament formation and actomyosin contractility (AMC) have been reported in response to TNF-alpha. These events are thought to play an important role in mediating TNF-alpha induced apoptosis but how they do so is unclear. In this study we prevented non-muscle myosin II activation in response to TNF-alpha by treating cells with the myosin light chain kinase (MLCK) inhibitor ML-7 or through isoform specific siRNA knockdown of myosin IIA and IIB. We found that treatment with ML-7 or knockdown of myosin IIB, but not IIA, impaired the cleavage of caspase 3 and caspase 8 as well as nuclear condensation in response to TNF-alpha. During this cell death process myosin II seemed to function independent of AMC since treatment of cells with blebbistatin or cytochalasin D failed to inhibit TNF-alpha induced caspase cleavage. Immunoprecipitation studies revealed associations of myosin IIB with clathrin and FADD in response to TNF-alpha suggesting a role for myosin IIB in TNFR1 endocytosis and DISC formation. Taken together these findings suggest that myosin IIB activation promotes TNF-alpha cell death signaling in a manner independent of its force generating property.
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Rydblom, Oskar. "You still say hello! : A corpus study of the greeting words hi, hey and hello in spoken American English." Thesis, Linnéuniversitetet, Institutionen för språk (SPR), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-33962.
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This study examines the usage of the three greetings words hi, hey and hello incombination with greeting questions, such as how are you, and terms of address, such asBill, Mr Jones or Dad, in spoken American English. The investigation identifies someof the patterns and rules that govern greetings sequences and to what extent socialfactors like gender, age, social class and social context inform the speaker‘s choice ofgreeting. Theories within the area were then used to identify greetings in the LongmanSpoken American English Corpus that were then entered into a database along withinformation about the speakers. Using filters the greeting pairs where then sorted bydifferent factors, such as gender, to extract quantitative data on the distribution andfrequency of usage.The results showed variation influenced by social factors and formulaic patternsof greeting sequences. The study concludes that hi is the standard and most frequentgreeting in spoken American English, hey is a more vernacular form and hello seems tobe more frequently used when respecting the boundaries of the addressee. Greetingsequences also follow a predictable pattern based on the greeting word used or thegreeting questioned asked. More formal terms of address (for example Mr Lastname)are used in more formal settings or when there is a significant age difference betweenspeakers. If a greeting question (how are you?) is posed it is usually returned, answeredor both, and if answered often with a short reply providing minimal information (fine).Hence, both the choice of greeting word and the use of terms of address and greetingquestions are influenced by social factors like age, gender, social class and formality ofsetting according to observable patterns.hi, hey, hello, greetings, adjacency pairs, politeness, Longman Spoken American English Corpus, corpus linguistics, sociolinguistics, phraseology
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Rodríguez, Eduardo. "Virion- and VAP-receptor recognition in the human adenovirus type 2 system." Lund : Lund University, 1998. http://catalog.hathitrust.org/api/volumes/oclc/68945080.html.
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Billing, Mischa. "Hela jag fylls av sommarvarm frukt." Örebro universitet, Restaurang- och hotellhögskolan, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-29898.
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Tinz, Susse, and Vivi-Ann Eliasson. "Jag blir inte snead hela tiden." Thesis, Malmö högskola, Lärarutbildningen (LUT), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-30385.
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Eliasson, Vivi-Ann & Tinz, Susse (2012). ”Jag blir inte snead hela tiden” – Gymnasieelever med diagnos dyslexi resonerar kring datorn som lärverktyg i klassrummet [So I won’t be eyeballed all the time – Students in secondary school, diagnosed with dyslexia, reason about the computer as a learning tool in the classroom]. Skolutveckling och ledarskap, Specialpedagogik, Lärarutbildningen, Malmö Högskola. Handledare: Lisbeth Ohlsson. Examinator: Kristian Lutz.ProblemområdeSkolans styrdokument är tydliga i sina ansatser att underlätta för elever i behov av särskilt stöd oavsett om de har diagnos eller inte. Det uppfattas ändå som ett problem att tillgodose alla elevers behov inom ramen för den ordinarie skolan, trots att skolans uppdrag att anpassa sig till elevernas olikheter Den person som inte känner sig delaktig i klassrummet är inte heller en del i gruppens demokratiska process. Syfte Denna studies syfte var att undersöka hur elever med dyslexi upplever att deras specifika situation påverkas i en lärmiljö där samtliga elever har var sin dator, samt hur dessa elever ser på möjligheten till inkludering i denna lärandemiljö. Studien fokuserar på elevers upplevelser av skolans sätt att organisera undervisningen. Detta görs genom att ställa frågor till sju gymnasieelever, med diagnos dyslexi, som går sitt andra eller tredje år på ett teoretiskt program. MetodStudien är kvalitativ och fokuserar på inkludering ur ett individperspektiv. För att öka studiens giltighet användes intervjuer, enkätfrågor samt klassrumsobservationer. Dessa analyserades och tolkades utifrån ett hermeneutiskt perspektiv. Analysarbetet utgick ifrån de kvalitetskriterier gällande inkludering, som har använts i flera tidigare forsknings-rapporter. Teoretisk ramDet sociokulturella perspektivet är centralt i studien. I tidigare forskning inom området inkludering ges en delvis negativ bild av inkludering för elever med diagnos dyslexi. Inom forskning som riktar sig mot informations- och kommunikationsteknologi, IKT, ges däremot en mer positiv bild av datorn som stöd, dels för lärande och dels för inkludering. Resultat med analysI denna studie, liksom i flera tidigare studier, visar resultaten att eleverna värdesätter kamratrelationer högt. De värdesätter också högt det faktum att de inte är ensamma om att ha en dator som lärverktyg. I denna studie används Farrells (2004) fyra kvalitetskriterier för begreppet inkludering; presence (närvaro), acceptance (acceptans), participation (aktivt deltagande) och achievement (utveckling av en positiv självbild). Resultat från klassrumsobservationer, enkätsvar och intervjuer ger en övervägande positiv bild av elevernas upplevelser i relation till inkludering i denna miljö där samtliga elever har dator som lärverktyg. Studien ger en bild av vilka faktorer som uppfattades som positiva respektive negativa av eleverna samt hur de resonerade kring inkludering. Underlaget är inte tillräckligt omfattande för att kunna dra några generella slutsatser. KonklusionStudien pekar på att det centrala inte är vad man gör för eleven, utan vad det resulterar i för eleven.
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Freitas, Ivania Garavello Forte. "Ação do laser de HeNe na osteogenese." [s.n.], 2001. http://repositorio.unicamp.br/jspui/handle/REPOSIP/261287.
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Orientadores : Vitor Baranauskas, Maria Alice da Cruz-HoflingTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Eletrica e de Computação
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Doutorado
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CARDON, BERTRAND. "Metatarsalgies statiques : a propos de 40 osteotomies de helal." Amiens, 1993. http://www.theses.fr/1993AMIEM009.
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Burgos, Rosa M. "Epigenetic Effects of Arsenite in HeLa Cells." Thesis, The University of Arizona, 2007. http://hdl.handle.net/10150/193356.
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Mechanisms of arsenic toxicity are not yet clear. Arsenite has effects on methylation pathways, by decreasing expression of DNA methylases and depletion of S-adenosylmethionine. Histones are DNA packing proteins that regulate gene expression modulating chromatin accessibility. Methylation at Lysine 9 of Histone H3 (K9H3) is a hallmark of heterochromatin. Dimethyl K9H3 is a mark of facultative heterochromatin and trimethyl K9H3 is present on constitutive heterochromatin. HeLa cells exposed for 24 hrs to 1 uM or 5 uM Sodium Arsenite were fixed and different posttranslational modifications of histones were detected by indirect immunofluorescence. Images were analyzed to assess the change on average methylated species of K9H3 in cell nuclei. Interestingly Arsenite (1 uM and 5 uM) treated cells had a significant increase in the trimethylated and dimethylated of K9H3, evaluated throught the comparison of average nuclei brightness and pixel value analysis between treatments.
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Thomas, David. "Elementary calcium release events in HeLa cells." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621734.
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Owler, David. "Uptake of cardiac glycosides by HeLa cells." Thesis, University of St Andrews, 1986. http://hdl.handle.net/10023/14072.
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Methods for the microcarrier culture of HeLa cells were developed and optimum culture conditions established. Methods were also established for the isolation of HeLa plasma membranes on beads, based on the principle of cell lysis followed by the shrearing away of the internal cell components, leaving the plasma membrane attached to the beads. The HeLa plasma membranes isolated on beads showed 5-8 fold enrichment of plasma membrane markers, while intracellular markers were depleted to the point of non-detection (except for some slight lysosomal contamination). The preparation time for isolation of HeLa plasma membranes on beads was about 1.5 hrs. The uptake of cardiac glycosides by HeLa cells was investigated. HeLa cells were labelled with [3H]-glycoside, the non-specific binding washed off and the cells returned to normal growth medium. At various time intervals the amount of [3H]-glycoside bound to HeLa plasma membranes and HeLa cells was determined. The rate of loss of ouabain and digoxin from HeLa plasma membranes was found to be about 10%hr-1. The excretion rates of ouabain and digoxin from HeLa cells were 4%hr-1 and 10%hr-1 respectively. The nature of the uptake and excretion processes were investigated using specific inhibitors of receptor-mediated endocytic processes. No inhibitor affected the rate of loss of cardiac glycoside from HeLa plasma membranes but monensin and chloroquine slowed the excretion rate of digoxin from HeLa cells. Modulation of sodium pump numbers by various chronic stress conditions - high serum concentration, low K medium and high K medium - were investigated. High concentrations of serum had no effect on the rate of loss ouabain from HeLa plasma membrane, but low K medium reduced the rate of loss of ouabain from membranes from 10%hr-1 to 4%hr-1. The low K medium also caused a x2 increase in sodium pump numbers in the plasma membrane. Increasing the serum concentration abolished the effects due to low K medium. High K medium also caused a reduction in the rate of loss of ouabain from plasma membranes from 10%hr-1 to 4%hr-1. The results obtained for low K medium were consistent with a model whereby sodium pumps in the plasma membrane are regulated by alteration of the sodium pump turnover rate (or internalisation rate).
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Lewin, Alyssa, and Stina Zetterdahl. "Hela Holma - En studie av ett grannskap." Thesis, Malmö högskola, Fakulteten för kultur och samhälle (KS), 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-22240.
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Syftet med denna masteruppsats är att undersöka bostadsområdet Holma, dess förutsättningar och hur det upplevs av befolkning och verksamma i området, samt att analysera och diskutera området utifrån teoretiska resonemang kring plats, urbanitet och grannskap. Vidare är förhoppningen att kunna identifiera resurser för främjandet av gemenskap och hållbar förbättring. Arbetet skrivs utifrån ett socialkonstruktionistiskt perspektiv där en premiss är att människor genom sitt agerande formar mening och att detta agerande i sig påverkas av sociala strukturer. Det empiriska arbetet utgörs av etnografiska studier som analyseras utifrån nämnd teoretisk bas. Studien mynnar ut i resonemang kring vikten av medborgarengagemang och långsiktighet i grundandet av intiativ i grannskapet. En viktig hållpunkt är att befolkningen bör ha möjlighet att själva identifiera och ha inflytande över sin plats då det är hos dem en grundlig kunskap om platsen finns. Examensarbetet visar hur de sociala resurser som verkar i området i högre grad bör användas för utvecklingen av ett gott grannskap.The purpose of this master thesis is to investigate the residential area Holma, Sweden, its preconditions and how it is experienced by the people that reside and work there, and to analyze and discuss this from theoretical viewpoints regarding space, place, urbanity and neighborhood. Furthermore, the aim is to identify resources for the promotion of community and sustainable improvement. The work is written from a social constructionist perspective in which one premise is that people, through their actions form meaning and that this behaviour itself is influenced by social structures. The empirical work consists of ethnographic studies that are further analyzed through the previously mentioned theoretical basis. The study culminates in discussions about the importance of civic engagement and long-term perspective in the implementation of the initiatives. A major strong point is that people should have the opportunity to identify and have influence over their place, as thorough knowledge of a place is to be found through its people. The thesis shows how the social resources that operate in the field should be better used for development of a strong neighborhood and community.
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Halvarsson, Sandra, and Ina Nilvén. "Studie- och yrkesvägledning som hela skolans ansvar." Thesis, Malmö högskola, Fakulteten för lärande och samhälle (LS), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-31403.
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Syftet med denna uppsats är att undersöka hur en grundskola arbetar för att implementera studie- och yrkesvägledning i vid bemärkelse, som hela skolans ansvar. Styrdokument beskriver att skolor bör arbeta med studie- och yrkesvägledning på ett långsiktigt och individanpassat sätt för att ge elever möjlighet att göra väl underbyggda val i framtiden. Ändå visar olika granskningar att utbudet av studie- och yrkesvägledning i grundskolan många gånger är bristande och av varierad kvalitet. En grundskola i Skåne arbetar i nuläget för att implementera studie- och yrkesvägledning under lektionstid, som ett projekt i skolorganisationen. Projektets implementering ska leda till att skolans elever ska få verktyg för framtida val men även att skolan aktivt ska arbeta med studie- och yrkesvägledning som ett gemensamt ansvar.Undersökningen genomfördes med en kvalitativ metod där intervjuer gjordes med fem lärare och en studie- och yrkesvägledare alla verksamma inom den undersökta skolorganisationen. Resultatet för undersökningen visar att skolan har kommit igång med projektet och börjat lägga en grund för etablering och vidareutveckling men även att skolans rektor har en betydande roll i projektet. Med uppsatsens teori Career Development Learning in School Systems som är en del av The Systems Theory Framework framkommer det i analysen hur skolan som system och dess delar så som rektor, lärare, studie- och yrkesvägledare och resurser är väsentliga för systemets helhet. Det framkommer dessutom att skolan påverkas av samhället och påverkar i sin tur detta. Det finns ständiga påverkningar inom och mellan dessa delar och system.
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Ujevic, Tonino Sebastian. "Análise de potenciais de interação nos sistemas de átomos de hélio." [s.n.], 2004. http://repositorio.unicamp.br/jspui/handle/REPOSIP/277166.
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Orientador: Silvio Antonio Sachetto VitielloTese (doutorado) - Universidade Estadual de Campinas, Instituto de Fisica Gleb Wataghin
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Resumo: Na primeira parte da tese, os efeitos das contribuições interatômicas de três corpos na equação de estado do 4 He são investigadas. Um potencial recente de dois corpos junto com o potencial de três corpos de Cohen e Murrell são utilizados para descrever o sistema de átomos de hélio. A dispersão de triplo dipolo e as energias de troca são estimadas sujeitas só a incertezas estatísticas. Uma extensão ao método de difusão com Monte Carlo é empregado para calcular diferenças de energias muito pequenas. Os resultados mostram como as contribuições de três corpos afetam a energia do estado fundamental, e as densidades de equilíbrio, solidificação e liquefação. Na investigação dos sistemas de hélio, diversos potenciais ab initio de dois corpos foram propostos na literatura. Nesta segunda parte da tese realizamos uma análise cuidadosa de como estes potenciais descrevem as propriedades do sistemas de 4 He usando a extensão proposta ao método de difusão com Monte Carlo. Esta extensão nos permitiu calcular com grande precisão pequenas diferenças de energia associadas a diferentes potenciais como função da densidade do sistema. Com estes resultados foi possível acompanhar cuidadosamente como a descrição das propriedades do sistema mudam com os potenciais de dois corpos considerados. Da nossa análise identificamos o potencial que em conjunto com interações de três corpos foi capaz de fornecer a melhor descrição dos sistemas de hélio
Abstract: In the first part of the thesis, the effect of three-body interatomic contributions in the equation of state of 4 He are investigated. A recent two-body potential together with the Cohen and Murrell three-body potential are applied to describe the sistem of helium atoms. The triple dipole dispersion and exchange energies are evaluated subjected only to statistical uncertainties. An extension of the diffusion Monte Carlo method is applied in order to compute very small energies differences. The results show how the three-body contributions affects the ground-state energy, the equilibrium, melting and freezing densities. In the investigation of the helium systems several ab initio two-body potentials have been propoused in the literature. In the second part of the thesis, we performed a carefull analysis of how these potentials describe the properties of the 4 He systems by using the proposed extension to the diffusion Monte Carlo Method. This extension have allowed the calculation of small energies differences, due to the different potentials, as a function of the system density with great accuracy. With our results it is possible to carefully follow how properties of the sistem change with the two-body potentials considered. From our analises we identify the potential that together with three-body interactions is able to give the best description of the helium systems
Doutorado
Física
Doutor em Ciências
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Sá, Luiz Henrique da Silva e. "Histórias de cenografia e design: a experiência de Helio Eichbauer." Universidade do Estado do Rio de Janeiro, 2008. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=1339.
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Partindo da obra e da experiência do cenógrafo brasileiro Helio Eichbauer, esta dissertação debate os possíveis conceitos e paradigmas do projeto em Cenografia através da história, relacionando a profissão do cenógrafo com a do designer devido às suas características de desenvolvimento de projetos. Trata de uma experiência didática desenvolvida nos anos 1970 no Rio de Janeiro, considerada como uma época de
ensino holístico do Design, da Cenografia e das Artes. Finalmente, estuda o caso da cenografia realizada para o espetáculo O rei da vela, de Oswald de Andrade, direção de
José Celso Martinez Corrêa, em 1967, mostrando a construção de significado a partir de sua composição espaço-visual.From the experience of Helio Eichbauers work stage design in Brazil, this study aims to discuss the chief points and paradigms of designing for the stage beyond the History, relating scenographers and designers based on their same acting in developing projects. This study shows an experience developed in the early 70s, in Rio de Janeiro, recognized as an holistic teaching of Design, Scenography and Arts. Lastly, the author studies the specific case of stage design for the play O rei da vela (The candle king), written by Oswald de Andrade and directed by José Celso Martinez Corrêa in 1967. He shows the creation process of meaning by the visual and space composition
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Gregoracci, Gustavo Bueno. "Terapia experimental com bacteriófagos." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317028.
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Orientador: Marcelo BrocchiTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Bacteriófagos são vírus que infectam bactérias e arqueias, representando as entidades biológicas mais abundantes do mundo e influenciando de maneira marcante populações naturais de seus hospedeiros. A terapia com bacteriófagos, que representa uma das primeiras formas modernas de combate a infecções bacterianas, foi recentemente redescoberta e vem sendo reavaliada seguindo metodologias atuais quanto à sua viabilidade terapêutica. Para completar a caracterização dos fagos de nossa coleção, sequenciamos completamente o genoma da maior parte destes, através da metodologia multiplex pair-ended utilizando a plataforma Illumina. Visando contribuir para verificação da viabilidade terapêutica de bacteriófagos testamos os efeitos protetor e terapêutico dos fagos Shfl1, Saen1v2 e Saen1v4, pertencentes à coleção de nosso laboratório, em modelos biológicos relevantes. O fago Shfl1, lítico contra Shigella flexneri, foi testado em ensaio de invasão em células HeLa. A redução de bactérias intracelulares foi mensurada independentemente através de plaqueamento e citometria de fluxo, além da observação direta por microscopia de fluorescência. O fago Saen1v2, lítico contra Salmonella Typhimurium, foi estudado quanto à biodistribuição e meia-vida em modelo murino, e uma variante viral com maior persistência in vivo foi selecionada. Essa variante, denominada Saen1v2p5, e o fago Saen1v4, lítico contra Salmonella Typhi, foram testados em modelo murino de infecção tifoide, contra seus respectivos hospedeiros. Encontramos similaridade genômica a fagos conhecidos, como T4, T7, T1 entre outros, em maior ou menor grau. Obtivemos um efeito protetor e terapêutico contra Shigella flexneri utilizando o fago Shfl1 em ensaio de invasão em cultura de células HeLa, verificado por todas as metodologias empregadas. Não verificamos efeito antimicrobiano in vivo do fago Saen1v2p5 em modelo murino de infecção por Salmonella Typhimurium. Por outro lado, observamos efeito terapêutico e protetor dose dependente utilizando o fago Saen1v4 em modelo murino de infecção por Salmonella Typhi. O sucesso obtido com baixas multiplicidades de infecção sugere um possível efeito indireto ou estimulação imune inespecífica
Abstract: Bacteriophages are viruses that infect Bacteria and Achaea, representing the most abundant biological entities in the world and markedly influencing natural host populations. Phage therapy, which represents one of the first modern ways to fight bacterial infections, was recently rediscovered and is being re-evaluated according to current methodologies regarding its therapeutic viability. In order to complete phage characterization in our collection, we sequenced completely the genomes of most of these, through the multiplex pair-ended methodology using the Illumina platform. Aiming to contribute to the therapeutic viability verification of bacteriophages we tested phage protective and therapeutic effects of Shfl1, Saen1v2 and Saen1v4, which belong to our collection, in biologically relevant models. Phage Shfl1, lytic against Shigella flexneri, was tested in a HeLa invasion assay. Intracellular bacteria reduction was measured independently through plating and flow cytometry, besides direct observation through fluorescent microscopy. Phage Saen1v2, lytic against Salmonella Typhimurium, was studied about its bio-distribution and half-life in murine model, and a viral variant with longer in vivo persistence was selected. This variant, denominated Saen1v2p5, and phage Saen1v4, lytic against Salmonella Typhi, were tested in murine typhoid model, against their respective hosts. Genomic similarity to known phages such as T4, T7, T1 among others, was found, in various degrees. We obtained both protective and therapeutic effect against Shigella flexneri using phage Shfl1 in the HeLa invasion assay, through all methodologies utilized. We could not verify in vivo antimicrobial effect of phage Saen1v2p5 in the murine model of Salmonella Typhimurium infection. On the other hand, we observed both therapeutic and protective dose dependent effect using phage Saen1v4 in Salmonella Typhi murine infection model. The success obtained with low multiplicities of infection may suggest a possible indirect effect or unspecific immune stimulation
Doutorado
Microbiologia
Doutor em Genetica e Biologia Molecular
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MATTEDI, R. B. P. "Convite ao caminhar: o lugar do encontro de Hélio Oiticica e Martin Heidegger." Universidade Federal do Espírito Santo, 2009. http://repositorio.ufes.br/handle/10/2068.
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Previous issue date: 2009-04-24Desenvolve uma tese sobre pontos em comum entre o pensamento de Martin Heidegger e a invenção dos trabalhos de Helio Oiticica. Esses nexos se estabelecem no tocante à questão do espaço e se dão, especialmente, nas propostas de Helio Oiticica intituladas Penetráveis. Na execução desses trabalhos, há uma confluência de pensamentos e de questões longamente analisadas e desenvolvidas em textos de Martin Heidegger, a saber, o lugar do homem no mundo, a experiência como caminho para a descoberta ou o desencobrimento do mundo tal como ele se dá, bem como o caminhar como experiência que propicia essa descoberta. Entende-se a ação obstinada de Helio Oiticica em construir lugares para criação do espaço, criação da experiência, de possibilitar um habitar poético, como uma tentativa de despertar o homem de uma espécie de sono cotidiano e colocá-lo no acontecimento das coisas, no mundo. Desenvolve-se este trabalho em três capítulos, nos quais se trata cronologicamente a produção de Helio Oiticica, a partir de sua experiência com o Grupo Neoconcreto, até o momento em que realiza os Penetráveis e os Projetos Ambientais que contam com a participação deles. Atendo-se mais demoradamente a essa série de trabalhos, tanto aos Penetráveis construídos em escala humana quanto às maquetes, aponta-se especialmente para a busca do espaço no trabalho do artista, mostrando como sua obra foi libertando-se, ganhando asas e encaminhando-se no e para o espaço. Com a construção desses lugares de experimentação, o artista cria espaços de possibilidade de reencontro do homem com o seu próprio, trazendo para sua obra uma prática de pensamento que se dá no caminhar, no estar, no viver e no experimentar fenomenológico.
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Hansson, Josefine, Jennie Larsson, and Sanna Nilsson. "You had me at hello : Om intuition vid rekrytering." Thesis, Linnéuniversitetet, Institutionen för organisation och entreprenörskap (OE), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-65532.
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In this qualitative report we have evaluated the significance intuition have on recruiters in their decision-making process. We were furthermore interested in what ways recruiters reflect on, and how they are handling intuition while recruiting. Ultimately our aim was to highlight potential risks and consequences that could emerge as a result of the use of intuition in recruitment. While using a deductive approach we conducted nine semi-structured interviews with recruiters representing various types of companies in Kalmar. Our empirical data combined with our theoretical framework yielded us substantial material in order to analyze our results. Through our investigation we have noticed the degree of intuition among recruiters as being highly individual. However, depending on what type of personality the recruiter has, would also make an impact on the recruiter’s stance toward intuition. In addition, experience and awareness about what role intuition plays in the recruitment process are aspects that contribute as to how the recruiters use their intuition. Due to negative implications that can be caused by unawareness of the impact of intuition, such as discrimination, homosocial reproduction or error recruitments, we find it necessary to emphasize intuition into a practical context at workplaces.
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Yanyan, Ke. "Plano de marketing para a marca Hello Bubble Waffle." Master's thesis, Instituto Superior de Economia e Gestão, 2019. http://hdl.handle.net/10400.5/18166.
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Mestrado em MarketingEste projeto tem como objetivo a formulação de um plano de marketing para 2020 visando o aumento da notoriedade do estabelecimento Hello Bubble Waffle por meio de aplicações de social media. Hello Bubble Waffle é um estabelecimento de sobremesas localizado em Lisboa, que se dedica exclusivamente a produzir uma sobremesa típica de Hong Kong - egg waffle no local e a vendê-la diretamente a consumidor final. O presente projeto é desenvolvida na área de planeamento de marketing em torno de 3 temas: planeamento de marketing, marketing de social media e word-of-mouth e notoriedade de marca. Com a utilização da combinação de métodos quantitativo e qualitativo, realizaram-se entrevistas semi-estruturadas face-a-face e um questionário online, visando recolher informações para fundamentar o plano de marketing. Com base na pesquisa realizada, foi obtida informação sobre: 1) a notoriedade atual e a notoriedade desejada da HBW; 2) as características do público alvo da HBW; 3) as aplicações de social media eficazes para lançar atividades de marketing; 4) os formatos de mensagens publicadas em social media que capturam a atenção; e 5) o efeito de word-of-mouth para a notoriedade de marca. Com todos os dados primários e secundários, formularam-se no final as estratégias de marketing mix de 4P (produto, preço, distribuição e comunicação), com ênfase na comunicação, a fim de aumentar a notoriedade de marca do estabelecimento Hello Bubble Waffle com recurso às aplicações de social media.
This project is aimed at formulating a marketing plan for 2020 to increase awareness through social media applications for a dessert shop in Lisbon - Hello Bubble Waffle (HBW), which focuses its operation totally on producing a typical dessert from Hong Kong - egg waffle on site and selling directly to final customers. The present project is developed in the marketing planning area around three topics: (1) marketing planning, (2) social media and word-of-mouth marketing, and (3) brand awareness. Both qualitative and quantitative approaches were used through semi-structured face-to-face interviews and online questionnaire, in order to collect information to better inform the marketing plan. Based on the research carried out, it was mainly collected information about 1) actual brand awareness and intended awareness of HBW; 2) characteristics of HBW's target public; 3) effective social media applications to launch marketing activities; 4) the formats of messages posted on social media which catch attention; 5) the effect of word-of-mouth to increase brand awareness. Based on both primary and secondary data, the strategies of 4P's marketing mix (product, price, place and promotion) were formulated, with emphasis on promotion, in order to increase brand awareness of Hello Bubble Waffle through social media applications.
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Fernandes, Adjaci Uchoa. "Síntese, caracterização e fotoatividade de fotossensibilizadores derivados de protoporfirina IX e de clorofilina." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/46/46135/tde-02072008-160723/.
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Processos que envolvem sensibilização são extremamente importantes para diversas áreas do conhecimento, incluindo a biologia, a química e a medicina. A aplicação de sensibilização em medicina tem se destacado, especialmente, em face de uma modalidade alternativa de tratamento de câncer denominada terapia fotodinâmica (TFD). Uma das linhas de pesquisas fundamentais para a evolução da terapia fotodinâmica é o desenvolvimento de novos fotossensibilizadores (Fs) com composição definida, que absorvam na janela terapêutica (600- 800nm) e que apresentem maior eficiência na indução de apoptose. Os Fs que apresentam cargas positivas e que são relativamente lipofílicos, permeiam membranas e são atraídos pelo potencial negativo das mitocôndrias, que tem papel central no controle da vida e da morte celular. Neste trabalho foi realizado um estudo da influência dos grupos funcionais na atividade dos Fs, através da funcionalização da protoporfirina IX (Pp IX). Foram estudadas três diferentes rotas sintéticas. Na rota I (esquema 14), utilizou-se como composto de partida a Hematoporfina IX (Hp IX), a qual foi funcionalizada com grupos aminas e amidas, respectivamente, nas hidroxilas e nas carboxilas. Esta rota forneceu baixo rendimento global (20%), e compostos de difícil purificação, no entanto obteve-se 1 composto puro. Na rota II (esquema 15), utilizou-se como composto de partida a Pp IX, a qual foi funcionalizada nos grupos vinílicos com grupos aminas. Obteve-se 4 compostos, com rendimento global de reação superior a 50%, mas observou-se que ocorre uma reação de eliminação que impede a quartenarização das aminas localizadas nas posições α ao anel porfirínico. Na rota III (esquema 16), os grupos carboxílicos de Pp IX foram transformados em cloreto de ácido, que foram substituídos por compostos bifuncionais, amina primária e um segundo grupo. Esta rota possibilitou a obtenção de 7 compostos, inclusive compostos quaternários com rendimento global de reação superior a 70%. Dois derivados da clorofilina, que apresentam a banda QIV com um ε elevado em 650nm, foram também sintetizados. Todos os compostos, foram caracterizados estruturalmente de forma inequívoca através do espectro eletrônico (UV-vis e fluorescência), vibracional no infravermelho, RMN de 1H e 13C (1D e 2D) e espectrometria de massa. A série de compostos obtidos permitiu um estudo da relação entre a estrutura química do Fs com a sua fotoatividade. As propriedades fotofísicas foram caracterizadas por espectro de absorção e de emissão, fotólise de relâmpago a laser, eficiência quântica de fluorescência e de geração de oxigênio singlete. Estas determinações indicaram que as propriedades fotofísicas dos Fs não foram consideravelmente alteradas no processo sintético. Foi determinada a formação de agregados em solução aquosa e o equilíbrio monômero agregado foi deslocado no sentido da formação do monômero na presença de micelas de CTAB e SDS e de soro fetal. Observou-se, que a contribuição para desagregação é mais eficiente quando a carga da micela é oposta à do Fs. Foi determinado o coeficiente de partição octanol/água (logPo/a) em função do pH e constatou-se que os compostos que têm carga líquida apresentam valores de logPo/a entre -1 e 1 na faixa de pH entre 3 e 10. A incorporação destes compostos em lipossomos, seguiu perfil esperado considerando a carga, o logPo/a e a característica anfifílica dos compostos sintetizadas. Genericamente a eficiência de morte celular fotoinduzida seguiu o perfil de incorporação das drogas em células HeLa. Os estudos comparativos da citolocalização foram realizados por co-encubação das porfirinas sintetizadas com rodamina 123 (Rd), que se concentra em mitocôndrias. A localização em organelas citoplasmáticas foi determinada através de imagens obtidas por microscopia de fluorescência confocal. A sobreposição da emissão da Rd foi de 80% e 31% com o composto catiônico PpNpNI e aniônico PpNetPO3, respectivamente, comprovando a localização mitocondrial do composto positivo.Processes that involve sensitization are extremely important for several areas of knowledge, including biology, chemistry and medicine. The application of sensitization in medicine is especially important, in face of an alternative modality of cancer treatment called Photodynamic Therapy (PDT). One of the lines of basic research important for the evolution of PDT is the development of new photosensitizers (PS) with defined composition, that absorb in the therapeutical window (600-800nm) and that present greater efficiency in the induction of apoptosis. Positively charged PS with the proper lipophilic/hidrophilic balance permeate membranes and are attracted by the negative potential of mitochondria, which is an organelle that has central roles in the control of cell life and death. In this work the influence of the functional groups in the activity of PS was carried out, through the functionalization of protoporphyrin IX (Pp IX). Three different synthetic routes were used. In route I (scheme 14), Hematoporphyrin IX (Hp IX), was used as departure compound and it was modified with amino and amide groups in hydroxyls and carboxyls, respectively. We found low yield in the synthetic (20%) and purification steps. However 1 pure PS was obtained. In route II (scheme 15), Pp IX was used as departure compound, which was modified in the vinilic groups with amino groups. We obtained 4 compounds, with global yield superior than 50%, but it was observed that an elimination reaction occurs that hinders the quarternization of the amino groups located in position α to the porphyrin ring. Into route III (scheme 16), the carboxilic groups of Pp IX had been transformed into chloride acid, which was substituted by bi- functional groups, primary amine in one side and another group in the other. This route made it possible to obtain 7 compounds, including quaternary ammonium compounds with global yield superior than 70%. Two derivatives of chlorophyllin, that present QIV band with large extinction coefficient in 650nm, also were synthesized. All compounds were characterized structurally through the electronic and vibrational spectra (UV-vis and fluorescence, IR), RMN of 1H and 13C (1D and 2D) and mass spectrometry. This series of compounds allowed us to study the relation between the chemical structure of the Fs with its photoactivity. The photophysical properties were characterized by emission and absorption spectra, laser flash photolysis, fluorescence emission in the visible and NIR regions (generation of singlet oxygen). These determinations indicated that the photophysical properties of the PS were not modified in the synthetic process. The formation of aggregates were characterized in aqueous solution and the balance between aggregate and monomer species was dislocated in the direction of the formation of monomers in the presence of CTAB, SDS micelles and fetal serum. It was observed, that the disaggregating efficient is larger with micelles that have the opposite charges to that of the PS. The Octanol water partition coefficient (logPo/a), was determined as a function of pH. logPo/a values between -1 and 1 were observed for charged PS in the pH range of 3-10. The incorporation of these compounds in liposomes, followed the expected profile considering the charge, logPo/a and the amphifilic nature. Generically, the photoinduced cell death efficiency followed the profile of incorporation of the PS in HeLa cells. Comparative studies of cytolocalization were carried out by co-incubation of the cells with porphyrins and Rhodamine 123 (Rd), which localizes in mitochondria. The localization in cytoplasmic organelles was determined through fluorescence confocal microscopy images. The overlapping emission of the Rd was of 80% and 31% with the cationic PpNpNI and the anionic PpNetPO3 compounds, respectively, proving the mitochondrial localization of the positive PS.
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Alsdal, Agnes, and Zin Kardas. "“Man måste tänka säkerhet hela, hela tiden” : - en kvalitativ studie om socialarbetares upplevelser av arbetet med hedersrelaterat våld och förtryck." Thesis, Örebro universitet, Institutionen för juridik, psykologi och socialt arbete, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-92724.
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Bravo, Sagua Roberto. "Regulación de la compartimentalización de la comunicación retículo endoplásmico-mitocondria en la línea tumoral hela." Tesis, Universidad de Chile, 2015. http://repositorio.uchile.cl/handle/2250/136838.
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Doctor en BioquímicaEl retículo endoplásmico (RE) y la mitocondria son organelos celulares que entablan una continua y dinámica conversación que permite a la célula responder coordinadamente a diversas situaciones fisiopatológicas. Datos previos de nuestro Laboratorio mostraron que ambos organelos incrementan los puntos de contacto físico durante la fase temprana del estrés de RE, permitiendo una mayor transferencia de Ca2+ desde el RE hacia la mitocondria y estimulando así el metabolismo de este último organelo y favoreciendo la sobrevida celular. El presente proyecto estudió la participación de proteína quinasa A (PKA) y caveolina-1 (Cav1) como agentes reguladores de esta interacción física y funcional entre el RE y la mitocondria. Como modelo de estudio se utilizaron células HeLa tratadas con tunicamicina como agente inductor de estrés de RE por 4 h. Primero, se investigó la participación de PKA en la fase temprana del estrés de RE. Esta quinasa se activó ante condiciones de estrés, medido por la fosforilación de DRP1 en Ser637 por Western blot y concomitante elongación mitocondrial (microscopía confocal). El inhibidor específico de PKA H89 previno estos cambios, comprobando que ambos son dependientes de PKA. Posteriormente, se estudió la participación de PKA en el acoplamiento RE-mitocondria a través de la medición de proximidad (microscopía confocal), contactos físicos (microscopía electrónica), transferencia de Ca2+ (microscopía de fluorescencia) y bioenergética mitocondrial (tasa de consumo de oxígeno). El papel de PKA en estos procesos se evaluó por modulación farmacológica con H89. Los resultados mostraron que la activación de PKA es necesaria para inducir la formación de contactos RE-mitocondria, y de esta forma, gatillar la respuesta adaptativa frente a estrés de RE. Luego se investigó el papel de Cav1 sobre la interface RE-mitocondria. La expresión de Cav1 abolió completamente la respuesta adaptativa frente a estrés de RE temprano, evidenciado mediante proximidad entre organelos, transferencia de Ca2+ y respiración mitocondrial. Además de alterar esta plasticidad organelar frente a condiciones de estrés, la expresión de Cav1 disminuyó significativamente la bioenergética mitocondrial basal, junto con inducir un remodelado basal de la interface RE-mitocondria medido por purificación de las membranas del RE asociadas a mitocondria (MAM). Finalmente, se determinó que la expresión de Cav1 antagonizó la señalización de PKA, impidiendo que ella fosforile a DRP1 y promueva la elongación mitocondrial en respuesta a estrés de RE. Más aún, mediante fraccionamiento subcelular, se estableció que Cav1 afectó la localización subcelular de PKA, evitando que se relocalice a las membranas microsomales en respuesta al estrés de RE. De este modo, Cav1 actúa como un regulador negativo de PKA, previniendo su activación frente a estrés de RE, evitando así la respuesta adaptativa celular
The endoplasmic reticulum (ER) and mitochondria are two organelles that continuously communicate between one another. This organelle crosstalk allows mitochondria and ER to coordinate responses to a variety of physiological and pathophysiological situations. Data from a previous work show that during the early phase of ER stress, both organelles increase their contact sites, augmenting thereby calcium transfer from ER to mitochondria. This response boosts mitochondrial bioenergetics and ultimately promotes cell survival. Here, we sought to study the role of Protein Kinase A (PKA) and Caveolin-1 (Cav1) as regulators of such organelle crosstalk. As an experimental model, HeLa cells were treated with tunicamycin to induce ER stress. First, we observed that PKA was activated during early stages of ER stress, as assessed by increased phosphorylation of DRP1 at the inhibitory site Ser637 (Western blot), and that this event was followed by mitochondrial elongation (confocal microscopy). The specific PKA inhibitor H89 prevented these changes, thus confirming that they were due to PKA and not another kinase. Subsequently, we studied ER-mitochondria coupling by evaluating organelle proximity (confocal microscopy), physical contact sites (electron microscopy), Ca2+ transfer (fluorescence microscopy) and mitochondrial bioenergetics (oxygen consumption rate). PKA was again involved in these processes by pharmacological modulation using the inhibitor H89. Our results show that PKA activation is necessary to induce the formation of ERmitochondria contacts and to trigger the adaptive metabolic responses to ER stress. Next, we investigated the role of Cav1 as a modulator of the ER-mitochondria interface. Cav1 overexpression completely abolished the early adaptive response to ER stress, as assessed by evaluating the proximity between organelles, calcium transfer and mitochondrial respiration. In addition to altering organelle plasticity in response to stress conditions, Cav1 overexpression severely decreased baseline mitochondrial bioenergetics and induced basal remodelling of the ER-mitochondria interface, as determined by purification and analysis of mitochondria-associated ER membranes. Finally, we determined that Cav1 overexpression antagonizes PKA signalling, preventing PKA-dependent DRP1 phosphorylation and mitochondrial elongation in response to ER stress. Moreover, subcellular fractionation revealed that Cav1 affected PKA localization, prevented redistribution to microsomal membranes in response to ER stress, and altered the pattern of DRP1 phosphorylation. Thus, Cav1 functions as a negative regulator of PKA that precludes PKA activation during early ER stress and thereby prevents the adaptive cellular response
Fondecyt; Fondap;
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Roslin, Anna. "Kiwimetoden – hela språkets metod : En studie om läsutveckling." Thesis, Karlstads universitet, Estetisk-filosofiska fakulteten, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-11764.
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Syftet med undersökningen är att ta reda på hur lärare genom att undervisa enligt kiwimetoden utvecklar elevers läsförmåga. Litteratur som rör hur barn lär sig läsa i allmänhet, och hur elever utvecklar läsförmåga genom kiwimetoden har lyfts fram. Empirin utgörs av kvalitativa intervjuer med två lärare, som har gedigen erfarenhet av kiwimetoden. Utöver det har också kompletterande observationer genomförts. Resultatet har presenterats i olika teman. Undersökningen visade att det är effektivt att lära ut lässtrategier, genom att visa och tänka högt dvs. genom att ”modella” hur en god läsare gör för att skapa förståelse för en text. Lässtrategier samt elevers förmågor i att formulera sig och kommunicera i tal och skrift, utvecklas genom att de tillsammans får diskutera och dissekera texter och bilder. Det visade sig också att elever som undervisats enligt kiwimetoden blir duktiga på att skriva, får ett utvecklat språk, samt upplever läsning som något positivt. För att arbeta med den vägledda läsningen krävs det emellertid att eleverna skolas in i metoden, vilket bland annat innebär att de behöver lära strategier, för att kunna komma igång och arbeta självständigt.This study aims to examine how teachers by educating in accordance to the kiwimethod can develop students' reading skills. Literature related to how children learn to read in general, and how students develop reading skills by the kiwimethod are presented. Empirical data consist of qualitative interviews with two teachers, who have extensive experience of the kiwimethod. Additional observations have also been implemented. The results have been presented in various themes. The investigation revealed that its effective to teach reading strategies, by viewing and thinking aloud, in other words to “model” what good readers do, when they create understanding of a text. A successful way of teaching readingstrategies also seems to be, by discussing and dissecting texts and images together. It was also found that students, who were taught, in accordance to the kiwimethod become good at writing, got a well-developed language, and experienced reading in a positive way. Working with guided reading however requires, that students receive training in working accordance to the method, which means that students need to learn strategies, that will help them to work independently.