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Journal articles on the topic 'Helicase Dependent Amplification (HDA)'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Uphoff, Timothy S., Blake W. Buchan, Nathan A. Ledeboer, Paul A. Granato, Judy A. Daly, and Tara N. Marti. "Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture." Journal of Clinical Microbiology 54, no. 9 (2016): 2388–90. http://dx.doi.org/10.1128/jcm.01268-16.

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We compared group AStreptococcus(GAS) culture with a rapid helicase-dependent amplification (HDA) method using 1,082 throat swab specimens. The HDA method demonstrated 98.2% sensitivity and 97.2% specificity. GAS prevalence by culture was 20.7%, and it was 22.6% using the HDA method. In 35 min, the HDA method provided rapid, sensitive GAS detection, making culture confirmation unnecessary.

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2

Wu, Xinghai, Chanfa Chen, Xizhi Xiao, and Ming Jun Deng. "Development of Reverse Transcription Thermostable Helicase-Dependent DNA Amplification for the Detection of Tomato Spotted Wilt Virus." Journal of AOAC INTERNATIONAL 99, no. 6 (2016): 1596–99. http://dx.doi.org/10.5740/jaoacint.16-0132.

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Abstract A protocol for the reverse transcription-helicase-dependent amplification (RT–HDA) of isothermal DNA was developed for the detection of tomato spotted wilt virus (TSWV). Specific primers, which were based on the highly conserved region of the N gene sequence in TSWV, were used for the amplification of virus's RNA. The LOD of RT–HDA, reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP), and reverse transcriptase-polymerase chain reaction (RT-PCR) assays were conducted using 10-fold serial dilution of RNA eluates. TSWV sensitivity in RT–HDA and RT-LAMP was 4 pg RNA com

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3

Kankanit, Pisamayarom, and Chaumpluk Piyasak. "RAPID LISTERIA MONOCYTOGENES ASSAY BASED ON HELICASE DEPENDENT AMPLIFICATION (HDA) AND NUCLEIC ACID HYBRIDIZATION IN BLUE SILVER NANOPLATES." International Journal of Research - Granthaalayah 5, no. 10 (2017): 322–35. https://doi.org/10.5281/zenodo.1043427.

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Listeria monocytogenes, a foodborne pathogen, is considered as one of the major problems in food safety. With strong safety regulations, a monitoring measure is essential for protecting the health and safety of consumers. Thus, a reliable monitoring method is required. In this study, a rapid assay based on a combination of helicase dependent amplification (HDA) and DNA signal detection via nucleic acid hybridization in blue silver nanoplates (AgNPls) was established. The assay started directly after short term enrichment in terrific broth using cotton ball swapping technique on seafood surface

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4

Padzil, Faiz, Abdul Razak Mariatulqabtiah, Wen Siang Tan, et al. "Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research." Animals 12, no. 1 (2021): 76. http://dx.doi.org/10.3390/ani12010076.

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Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic acid screening methods such as polymerase chain reaction (PCR), quantitative (real-time) polymerase chain reaction (qPCR) and reverse-transcription qPCR (RT-qPCR). While such molecular detection methods are widely utilized as the benchmark, the invention of isothermal amplifications has also emerged

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5

Pisamayarom, Kankanit, and Piyasak Chaumpluk. "RAPID LISTERIA MONOCYTOGENES ASSAY BASED ON HELICASE DEPENDENT AMPLIFICATION (HDA) AND NUCLEIC ACID HYBRIDIZATION IN BLUE SILVER NANOPLATES." International Journal of Research -GRANTHAALAYAH 5, no. 10 (2017): 322–35. http://dx.doi.org/10.29121/granthaalayah.v5.i10.2017.2308.

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Listeria monocytogenes, a foodborne pathogen, is considered as one of the major problems in food safety. With strong safety regulations, a monitoring measure is essential for protecting the health and safety of consumers. Thus, a reliable monitoring method is required. In this study, a rapid assay based on a combination of helicase dependent amplification (HDA) and DNA signal detection via nucleic acid hybridization in blue silver nanoplates (AgNPls) was established. The assay started directly after short term enrichment in terrific broth using cotton ball swapping technique on seafood surface

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6

Li, Dawei, Bei Lv, Hao Zhang, Jasmine Yiqin Lee, and Tianhu Li. "Disintegration of cruciform and G-quadruplex structures during the course of helicase-dependent amplification (HDA)." Bioorganic & Medicinal Chemistry Letters 25, no. 8 (2015): 1709–14. http://dx.doi.org/10.1016/j.bmcl.2015.02.070.

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7

Zasada, Aleksandra Anna, Ewa Mosiej, Marta Prygiel, et al. "Detection of SARS-CoV-2 Using Reverse Transcription Helicase Dependent Amplification and Reverse Transcription Loop-Mediated Amplification Combined with Lateral Flow Assay." Biomedicines 10, no. 9 (2022): 2329. http://dx.doi.org/10.3390/biomedicines10092329.

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Rapid and accurate detection and identification of pathogens in clinical samples is essential for all infection diseases. However, in the case of epidemics, it plays a key role not only in the implementation of effective therapy but also in limiting the spread of the epidemic. In this study, we present the application of two nucleic acid isothermal amplification methods—reverse transcription helicase dependent amplification (RT-HDA) and reverse transcription loop-mediated amplification (RT-LAMP)—combined with lateral flow assay as the tools for the rapid detection of SARS-CoV-2, the etiologica

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8

Chemisova, O. S., O. A. Tsyrulina, A. L. Trukhachev, and A. K. Noskov. "Comparative analysis of methods for isothermal amplification of nucleic acids." Journal of microbiology, epidemiology and immunobiology 99, no. 1 (2022): 126–38. http://dx.doi.org/10.36233/0372-9311-176.

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In this review, methods for isothermal amplification of nucleic acids are considered and analyzed, in particular, loop isothermal amplification of DNA and RNA (LAMP/RT-LAMP), helicase-dependent amplification (HDA) and recombinase polymerase amplification (RPA). The advantages and disadvantages of each of the techniques are described. The possibility of their application in the molecular diagnostics of infectious diseases is evaluated. A brief review of the literature on the use of LAMP, HDA, RPA in the diagnostics of viral, bacterial infections and diseases of protozoal etiology was conducted.

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9

Buddhachat, Kittisak. "LAMP assay is an auxiliary tool for rapid and sensitive pathogen diagnosis in veterinary areas." Veterinary Integrative Sciences 20, no. 2 (2020): 247–51. http://dx.doi.org/10.12982/vis.2022.019.

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Molecular technique for nucleic acid amplification has become more advanced, especially isothermal nucleic acid amplification which can amplify DNA or RNA molecules at constant temperatures. This allows us to be independent of sophisticated equipment such as thermal cyclers or realtime-PCR. The feasibility of adopting molecular techniques for pathogen detection at point-of-care are approaching practical use. At present, there are various isothermal nucleic acid amplification technologies, including rolling cycle amplification (RCA), helicase-dependent amplification (HDA), recombinase polymeras

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10

Faizul Rahman, S., Z. Nor Amalina, I. Asma, and I. Aziah. "Asymmetric helicase-dependent amplification combined with lateral flow assay (HDA-LFA) for the detection of S. Paratyphi A." Asian Pacific Journal of Tropical Disease 4, no. 3 (2014): 243. http://dx.doi.org/10.1016/s2222-1808(14)60551-x.

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11

Andreason, Sharon A., Mohammad Arif, Judith K. Brown, Francisco Ochoa-Corona, and Astri Wayadande. "Exploring the Use of High-Resolution Melting Analysis and Helicase-Dependent Amplification for Discrimination of Bemisia tabaci (Hemiptera: Aleyrodidae) Cryptic Species and Trialeurodes vaporariorum." Journal of Economic Entomology 113, no. 5 (2020): 2511–20. http://dx.doi.org/10.1093/jee/toaa180.

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Abstract The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera; Aleyrodidae), and greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae), are highly problematic plant pests and virus vectors with worldwide distributions. Identification of whitefly species is typically accomplished by observation of distinct morphological characters; however, because of morphological inconsistency and indistinguishability, the discrimination of B. tabaci species variants is dependent on molecular techniques based on genetic differences. New assays were designed for the dete

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12

Leonardo, Sandra, Anna Toldrà, and Mònica Campàs. "Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring." Sensors 21, no. 2 (2021): 602. http://dx.doi.org/10.3390/s21020602.

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The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand, biosensors based on isothermal DNA amplification methods have emerged, which avoid the need for thermal cycling, thus facilitating their integration into small and low-cost devices for in situ monitoring. This review focuses on the breakthroughs made on biosensors based on isothermal amplification methods for the detection of bacteria in the

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13

Arif, M., G. S. Aguilar-Moreno, A. Wayadande, J. Fletcher, and F. M. Ochoa-Corona. "Primer Modification Improves Rapid and SensitiveIn Vitroand Field-Deployable Assays for Detection of High Plains Virus Variants." Applied and Environmental Microbiology 80, no. 1 (2013): 320–27. http://dx.doi.org/10.1128/aem.02340-13.

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ABSTRACTA high consequence pathogen,High plains virus(HPV) causes considerable damage to wheat if the crop is infected during early stages of development. Methods for the early, accurate, and sensitive detection of HPV in plant tissues are needed for the management of disease outbreaks and reservoir hosts. In this study, the effectiveness of five methods—real-time SYBR green and TaqMan reverse transcription-quantitative PCR (RT-qPCR), endpoint RT-PCR, RT-helicase dependent amplification (RT-HDA) and the Razor Ex BioDetection System (Razor Ex)—for the broad-range detection of HPV variants was e

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14

Teo, Jeanette W. P., Donald Chiang, Roland Jureen, and Raymond T. P. Lin. "Clinical evaluation of a helicase-dependant amplification (HDA)–based commercial assay for the simultaneous detection of HSV-1 and HSV-2." Diagnostic Microbiology and Infectious Disease 83, no. 3 (2015): 261–62. http://dx.doi.org/10.1016/j.diagmicrobio.2015.07.018.

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15

Jangra, Rohit K., MinKyung Yi, and Stanley M. Lemon. "DDX6 (Rck/p54) Is Required for Efficient Hepatitis C Virus Replication but Not for Internal Ribosome Entry Site-Directed Translation." Journal of Virology 84, no. 13 (2010): 6810–24. http://dx.doi.org/10.1128/jvi.00397-10.

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ABSTRACT DDX6 (Rck/p54) is an evolutionarily conserved member of the SF2 DEAD-box RNA helicase family that contributes to the regulation of translation and storage and the degradation of cellular mRNAs. It interacts with multiple proteins and is a component of the micro-RNA (miRNA)-induced silencing complex (miRISC). Since miRNA-122 (miR-122) is essential for efficient hepatitis C virus (HCV) replication, we investigated the requirement for DDX6 in HCV replication in cultured hepatoma cells. Small interfering RNA (siRNA)-mediated knockdown of DDX6 and rescue with an siRNA-resistant mutant demo

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16

Liu, Yongru, Xi Wu, Yunlu Feng, et al. "Insights into the Oncogenic, Prognostic, and Immunological Role of BRIP1 in Pan-Cancer: A Comprehensive Data-Mining-Based Study." Journal of Oncology 2023 (April 28, 2023): 1–19. http://dx.doi.org/10.1155/2023/4104639.

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Background. BRCA1 interacting helicase 1 (BRIP1), an ATP-dependent DNA helicase which belongs to an Iron-Sulfur (Fe-S) helicase cluster family with a DEAH domain, plays a key role in DNA damage and repair, Fanconi anemia, and development of several cancers including breast and ovarian cancer. However, its role in pan-cancer remains largely unknown. Methods. BRIP1 expression data of tumor and normal tissues were downloaded from the Cancer Genome Atlas, Genotype-Tissue Expression, and Human Protein Atlas databases. Correlation between BRIP1 and prognosis, genomic alterations, and copy number var

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17

Vincent, Myriam, Yan Xu, and Huimin Kong. "Helicase‐dependent isothermal DNA amplification." EMBO reports 5, no. 8 (2004): 795–800. http://dx.doi.org/10.1038/sj.embor.7400200.

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18

Jeong, Yong-Joo, Kkothanahreum Park, and Dong-Eun Kim. "Isothermal DNA amplification in vitro: the helicase-dependent amplification system." Cellular and Molecular Life Sciences 66, no. 20 (2009): 3325–36. http://dx.doi.org/10.1007/s00018-009-0094-3.

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19

Motré, Aurélie, Ying Li, and Huimin Kong. "Enhancing helicase-dependent amplification by fusing the helicase with the DNA polymerase." Gene 420, no. 1 (2008): 17–22. http://dx.doi.org/10.1016/j.gene.2008.04.017.

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20

An, Lixin, Wen Tang, Tamara A. Ranalli, Hyun-Jin Kim, Jamie Wytiaz, and Huimin Kong. "Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification." Journal of Biological Chemistry 280, no. 32 (2005): 28952–58. http://dx.doi.org/10.1074/jbc.m503096200.

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21

Shetty, Prasad, Dipayan Ghosh, Minakshi Singh, Aparna Tripathi, and Debjani Paul. "Rapid amplification of Mycobacterium tuberculosis DNA on a paper substrate." RSC Advances 6, no. 61 (2016): 56205–12. http://dx.doi.org/10.1039/c6ra07529k.

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22

Ma, Fei, Meng Liu, Bo Tang, and Chun-yang Zhang. "Sensitive Quantification of MicroRNAs by Isothermal Helicase-Dependent Amplification." Analytical Chemistry 89, no. 11 (2017): 6182–87. http://dx.doi.org/10.1021/acs.analchem.7b01113.

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23

Andresen, Dennie, Markus von Nickisch-Rosenegk, and Frank F. Bier. "Helicase-dependent amplification: use in OnChip amplification and potential for point-of-care diagnostics." Expert Review of Molecular Diagnostics 9, no. 7 (2009): 645–50. http://dx.doi.org/10.1586/erm.09.46.

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24

Tupik, A. N., G. E. Rudnitskaya, T. A. Lukashenko, and A. A. Evstrapov. "Helicase-Dependent Isothermal Amplification of Nucleic Acids on Microfluidic Array Chip." Biotekhnologiya 34, no. 5 (2018): 77–85. http://dx.doi.org/10.21519/0234-2758-2018-34-5-77-85.

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25

Andresen, Dennie, Markus von Nickisch-Rosenegk, and Frank F. Bier. "Helicase dependent OnChip-amplification and its use in multiplex pathogen detection." Clinica Chimica Acta 403, no. 1-2 (2009): 244–48. http://dx.doi.org/10.1016/j.cca.2009.03.021.

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26

Mahalanabis, Madhumita, Jaephil Do, Hussam ALMuayad, Jane Y. Zhang, and Catherine M. Klapperich. "An integrated disposable device for DNA extraction and helicase dependent amplification." Biomedical Microdevices 12, no. 2 (2010): 353–59. http://dx.doi.org/10.1007/s10544-009-9391-8.

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27

Kivlehan, Francine, François Mavré, Luc Talini, Benoît Limoges, and Damien Marchal. "Real-time electrochemical monitoring of isothermal helicase-dependent amplification of nucleic acids." Analyst 136, no. 18 (2011): 3635. http://dx.doi.org/10.1039/c1an15289k.

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28

Jiang, Li-Li, Ka-Lok Wong, Yuk-Lau Wong, et al. "Helicase-dependent amplification is effective in distinguishing Asian ginseng from American ginseng." Food Control 43 (September 2014): 199–205. http://dx.doi.org/10.1016/j.foodcont.2014.03.016.

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29

Gill, P., H. Abdul-Tehrani, A. Ghaemi, T. Hashempour, A. Alvandi, and M. Noori-Daloii. "P587 Thermophilic helicase-dependent isothermal DNA amplification for molecular detection of Helicobacter pylori." International Journal of Antimicrobial Agents 29 (March 2007): S135—S136. http://dx.doi.org/10.1016/s0924-8579(07)70430-2.

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30

Torres-Chavolla, Edith, and Evangelyn C. Alocilja. "Nanoparticle based DNA biosensor for tuberculosis detection using thermophilic helicase-dependent isothermal amplification." Biosensors and Bioelectronics 26, no. 11 (2011): 4614–18. http://dx.doi.org/10.1016/j.bios.2011.04.055.

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31

Mahalanabis, Madhumita, Jaephil Do, Hussam ALMuayad, Jane Y. Zhang, and Catherine M. Klapperich. "Erratum to: An integrated disposable device for DNA extraction and helicase dependent amplification." Biomedical Microdevices 13, no. 3 (2011): 599–602. http://dx.doi.org/10.1007/s10544-011-9518-6.

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32

Faron, Matthew L., Nathan A. Ledeboer, Paul Granato, et al. "Detection of Group A Streptococcus in Pharyngeal Swab Specimens by Use of the AmpliVue GAS Isothermal Helicase-Dependent Amplification Assay." Journal of Clinical Microbiology 53, no. 7 (2015): 2365–67. http://dx.doi.org/10.1128/jcm.01085-15.

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We evaluated the clinical performance (sensitivity and specificity) of the AmpliVue group AStreptococcus(GAS) isothermal helicase-dependent amplification assay using 1,192 pharyngeal swab specimens. AmpliVue GAS assay results were compared to the results of routine throat cultures on selective streptococcal blood agar plates. The sensitivity and specificity of the AmpliVue GAS assay were 98.3% (95% confidence interval [CI], 95 to 100%) and 93.2% (95% CI, 91 to 95%), respectively.

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33

Oriero, E. C., J. P. Van Geertruyden, J. Jacobs, U. D’Alessandro, and D. C. Nwakanma. "Comparison of two isothermal amplification methods: Thermophilic helicase dependent amplification (tHDA) and loop mediated isothermal amplification (LAMP) for detection of Plasmodium falciparum." International Journal of Infectious Diseases 21 (April 2014): 381. http://dx.doi.org/10.1016/j.ijid.2014.03.1206.

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34

Bruck, Irina, Nalini Dhingra, and Daniel L. Kaplan. "A Positive Amplification Mechanism Involving a Kinase and Replication Initiation Factor Helps Assemble the Replication Fork Helicase." Journal of Biological Chemistry 292, no. 8 (2017): 3062–73. http://dx.doi.org/10.1074/jbc.m116.772368.

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The assembly of the replication fork helicase during S phase is key to the initiation of DNA replication in eukaryotic cells. One step in this assembly in budding yeast is the association of Cdc45 with the Mcm2–7 heterohexameric ATPase, and a second step is the assembly of the tetrameric GINS (GG-Ichi-Nii-San) complex with Mcm2–7. Dbf4-dependent kinase (DDK) and S-phase cyclin-dependent kinase (S-CDK) are two S phase-specific kinases that phosphorylate replication proteins during S phase, and Dpb11, Sld2, Sld3, Pol ϵ, and Mcm10 are factors that are also required for replication initiation. How

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35

Doseeva, Victoria, Thomas Forbes, John Wolff, et al. "Multiplex isothermal helicase-dependent amplification assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae." Diagnostic Microbiology and Infectious Disease 71, no. 4 (2011): 354–65. http://dx.doi.org/10.1016/j.diagmicrobio.2011.08.021.

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36

Andress, Arlise, Yanxia Bei, Bryan R. Fonslow, et al. "Spindle-E cycling between nuage and cytoplasm is controlled by Qin and PIWI proteins." Journal of Cell Biology 213, no. 2 (2016): 201–11. http://dx.doi.org/10.1083/jcb.201411076.

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Transposable elements (TEs) are silenced in germ cells by a mechanism in which PIWI proteins generate and use PIWI-interacting ribonucleic acid (piRNA) to repress expression of TE genes. piRNA biogenesis occurs by an amplification cycle in microscopic organelles called nuage granules, which are localized to the outer face of the nuclear envelope. One cofactor required for amplification is the helicase Spindle-E (Spn-E). We found that the Spn-E protein physically associates with the Tudor domain protein Qin and the PIWI proteins Aubergine (Aub) and Argonaute3 (Ago3). Spn-E and Qin proteins are

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37

Frech, Georges C., Denton Munns, Robert D. Jenison, and Brian J. Hicke. "Direct detection of nasal Staphylococcus aureus carriage via helicase-dependent isothermal amplification and chip hybridization." BMC Research Notes 5, no. 1 (2012): 430. http://dx.doi.org/10.1186/1756-0500-5-430.

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38

Du, Xin-jun, Tian-jiao Zhou, Ping Li, and Shuo Wang. "A rapid Salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay." Molecular and Cellular Probes 34 (August 2017): 37–44. http://dx.doi.org/10.1016/j.mcp.2017.05.004.

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39

Gill, Pooria, Amir-Houshang Alvandi, Hossein Abdul-Tehrani, and Majid Sadeghizadeh. "Colorimetric detection of Helicobacter pylori DNA using isothermal helicase-dependent amplification and gold nanoparticle probes." Diagnostic Microbiology and Infectious Disease 62, no. 2 (2008): 119–24. http://dx.doi.org/10.1016/j.diagmicrobio.2008.05.003.

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40

Barbieri, Daniela, Simona Venturoli, Frank Rösl, and Bladimiro Rincon-Orozco. "Detection of high-risk human papillomavirus type 16 and 18 using isothermal helicase-dependent amplification." Diagnostic Microbiology and Infectious Disease 79, no. 2 (2014): 178–82. http://dx.doi.org/10.1016/j.diagmicrobio.2014.02.012.

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41

Barreda-García, Susana, María José González-Álvarez, Noemí de-los-Santos-Álvarez, Juan José Palacios-Gutiérrez, Arturo J. Miranda-Ordieres, and María Jesús Lobo-Castañón. "Attomolar quantitation of Mycobacterium tuberculosis by asymmetric helicase-dependent isothermal DNA-amplification and electrochemical detection." Biosensors and Bioelectronics 68 (June 2015): 122–28. http://dx.doi.org/10.1016/j.bios.2014.12.029.

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42

Yang, Zunyi, Chris McLendon, Daniel Hutter, et al. "Helicase-Dependent Isothermal Amplification of DNA and RNA by Using Self-Avoiding Molecular Recognition Systems." ChemBioChem 16, no. 9 (2015): 1365–70. http://dx.doi.org/10.1002/cbic.201500135.

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43

Gill, Pooria, Mohsen Amini, Amir Ghaemi, et al. "Detection of Helicobacter pylori by enzyme-linked immunosorbent assay of thermophilic helicase-dependent isothermal DNA amplification." Diagnostic Microbiology and Infectious Disease 59, no. 3 (2007): 243–49. http://dx.doi.org/10.1016/j.diagmicrobio.2007.05.005.

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44

Nakahara, Tomomi, Katsuyuki Tanaka, Shin-ichi Ohno, Nagayasu Egawa, Takashi Yugawa та Tohru Kiyono. "Activation of NF-κB by Human Papillomavirus 16 E1 Limits E1-Dependent Viral Replication through Degradation of E1". Journal of Virology 89, № 9 (2015): 5040–59. http://dx.doi.org/10.1128/jvi.00389-15.

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ABSTRACTNF-κB is a family of transcription factors that regulate gene expression involved in many processes, such as the inflammatory response and cancer progression. Little is known about associations of NF-κB with the human papillomavirus (HPV) life cycle. We have developed a tissue culture system to conditionally induce E1-dependent replication of the human papillomavirus 16 (HPV16) genome in human cervical keratinocytes and found that expression of HPV16 E1, a viral helicase, results in reduction of IκBα and subsequent activation of NF-κB in a manner dependent on helicase activity. Exogeno

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45

Eckert, C., E. Holscher, A. Petit, V. Lalande, and F. Barbut. "Molecular Test Based on Isothermal Helicase-Dependent Amplification for Detection of the Clostridium difficile Toxin A Gene." Journal of Clinical Microbiology 52, no. 7 (2014): 2386–89. http://dx.doi.org/10.1128/jcm.00353-14.

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46

Barreda-García, S., R. Miranda-Castro, N. de-los-Santos-Álvarez, A. J. Miranda-Ordieres, and M. J. Lobo-Castañón. "Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable oligonucleotide-modified ITO electrodes." Chemical Communications 53, no. 70 (2017): 9721–24. http://dx.doi.org/10.1039/c7cc05128j.

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47

Cao, Anping, and Chun-yang Zhang. "Real-Time Detection of Transcription Factors Using Target-Converted Helicase-Dependent Amplification Assay with Zero-Background Signal." Analytical Chemistry 85, no. 4 (2013): 2543–47. http://dx.doi.org/10.1021/ac400010r.

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48

Ryabov, Eugene V. "A novel virus isolated from the aphid Brevicoryne brassicae with similarity to Hymenoptera picorna-like viruses." Journal of General Virology 88, no. 9 (2007): 2590–95. http://dx.doi.org/10.1099/vir.0.83050-0.

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A novel virus, Brevicoryne brassicae virus (BrBV), has been identified in the cabbage aphid using a method based on the random amplification of encapsidated RNA. The complete sequence of the RNA genome of BrBV has been determined. The positive-strand genomic RNA is 10 161 nt, excluding the 3′ poly(A) tail, and contains a single open reading frame (positions 793–9744) encoding a putative polyprotein of 2983 aa. The N-terminal part of the polyprotein shows similarity with the structural proteins of iflaviruses. The C-terminal part possesses consensus sequences of the helicase, cysteine protease

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Mbanefo, Afoma, and Nirbhay Kumar. "Evaluation of Malaria Diagnostic Methods as a Key for Successful Control and Elimination Programs." Tropical Medicine and Infectious Disease 5, no. 2 (2020): 102. http://dx.doi.org/10.3390/tropicalmed5020102.

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Malaria is one of the leading causes of death worldwide. According to the World Health Organization’s (WHO’s) world malaria report for 2018, there were 228 million cases and 405,000 deaths worldwide. This paper reviews and highlights the importance of accurate, sensitive and affordable diagnostic methods in the fight against malaria. The PubMed online database was used to search for publications that examined the different diagnostic tests for malaria. Currently used diagnostic methods include microscopy, rapid diagnostic tests (RDT), and polymerase chain reaction (PCR). Upcoming methods were

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Deng, Wentao, Biing Yuan Lin, Ge Jin, et al. "Cyclin/CDK Regulates the Nucleocytoplasmic Localization of the Human Papillomavirus E1 DNA Helicase." Journal of Virology 78, no. 24 (2004): 13954–65. http://dx.doi.org/10.1128/jvi.78.24.13954-13965.2004.

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ABSTRACT Cyclin-dependent kinases (CDKs) play key roles in eukaryotic DNA replication and cell cycle progression. Phosphorylation of components of the preinitiation complex activates replication and prevents reinitiation. One mechanism is mediated by nuclear export of critical proteins. Human papillomavirus (HPV) DNA replication requires cellular machinery in addition to the viral replicative DNA helicase E1 and origin recognition protein E2. E1 phosphorylation by cyclin/CDK is critical for efficient viral DNA replication. We now show that E1 is phosphorylated by CDKs in vivo and that phosphor

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