Academic literature on the topic 'Hemolys'

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Journal articles on the topic "Hemolys"

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Kanias, Tamir, Janet Lee, Mark H. Yazer, Darrell J. Triulzi, Ashley Lippert, and Mark T. Gladwin. "Correlation Between Female Gender and the Red Blood Cell Propensity to Hemolyze Under Various Stresses." Blood 118, no. 21 (November 18, 2011): 2325. http://dx.doi.org/10.1182/blood.v118.21.2325.2325.

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Abstract Abstract 2325 Red blood cell (RBC) hemolysis during hypothermic storage is a principal criterion for assessing the quality of RBC units. The presence of free hemoglobin in RBC units can promote post transfusion injuries, such as vascular dysfunction, via accelerated nitric oxide scavenging and reactive oxygen species. It has been noted in clinical practice that specific donor red cell units are more likely to hemolyze during storage, and that female gender is associated with less hemolysis following 42 days of hypothermic storage, and during experimental mechanical stress. These findings lead us to our overarching hypothesis that genetic variations, including gender, modulate the propensity of RBCs to hemolyze under diverse stressors that may be maximized during red blood cell storage. This study further examines the relationship between gender and hemolysis in human and mouse RBCs. Washed RBCs were obtained from RBC units stored in ADSOL for short (3–10 days old) or long (42–47 days old, expired units) time periods. The donors' age averaged 43.9±14.8 and 39.3±14.6 years, women versus men, respectively. RBCs were also obtained from 10-week-old C57BL/6J male and female mice and stored for 3 days in phosphate buffered saline. Hemolytic propensity was assessed by various stressors validated in our laboratory for 96-well plate high throughput assays. Osmotic stress was induced by incubating RBCs in hypotonic buffer containing glycerol. Oxidative hemolysis was induced by 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH). Mechanical stress was achieved by shaking RBC suspensions in the presence of a 3/32” stainless steel bead over 90 minutes. Percent hemolysis was measured by supernatant cell-free hemoglobin using Drabkin's assay. Hemolysis levels (%) in response to osmotic stress were significantly (p=0.0108, Mann-Whitney test) lower in RBCs donated by women and stored for 8±2 days (16±7.1 % versus 24±7.3 % in men, n=14). This difference was also observed in expired units (31±6.0 % versus 40±6.1 % in men, n=12-16, p=0.0017). The mechanical fragility index (MFI) of premenopausal women RBCs stored for 10 days was significantly (p=0.0286) lower than that of men from a matched age group (MFI= 7.3±0.9 versus 9.4±1, respectively; n=4). Conversely, women RBC response to AAPH-induced oxidative hemolysis treatment was similar to that of men (49±4.8 % versus 48±4.7 %, respectively, n=14). Similar assays done in C57BL/6J mice revealed that female RBCs hemolyze less in response to osmotic stress (60±5.6 % versus 69±8.4 % in males, n=10, p=0.0435) or AAPH treatments (58±6 % versus 65±3.3 % in males, n=10, p=0.0106). These gender differences became noticeable after 2–3 days of storage in PBS. Our study correlates gender with the propensity of RBCs to hemolyse under various stresses in human and mouse. It implies that under the tested conditions, RBCs collected by women are more resilient to mechanical and osmotic stresses, whereas female mouse RBCs exhibit higher resistance to osmotic and AAPH-induced oxidative stress compared with males. The mechanisms of this phenomenon are yet to be resolved and may be related to estrogen protective effects in reproductive females. Our experimental model suggests that gender differences are intrinsic to the RBC, as cells were washed and plasma or additive solution was removed. Understanding the molecular mechanisms of hemolysis can contribute to the process of RBC donor screening, reduce storage hemolysis, and possibly post-transfusion complications. Disclosures: Gladwin: Patents filed related to treating hemolysis.: Patents & Royalties.
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Zhang, Jing, Kai Chen, Chuanqi Jiang, Wentao Yang, Siyu Gu, Guangying Wang, Yishan Lu, Wei Miao, and Jie Xiong. "Bacteria-Derived Hemolysis-Related Genes Widely Exist in Scuticociliates." Microorganisms 8, no. 11 (November 22, 2020): 1838. http://dx.doi.org/10.3390/microorganisms8111838.

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Scuticociliatosis is an invasive external or systemic infection caused by ciliated protozoa, mainly those within the subclass Scuticociliatia (scuticociliates). Many scuticociliates are fish pathogens, including Miamiensis avidus, Philasterides dicentrarchi, Pseudocohnilembus persalinus, and Uronema marinum. Our previous study showed that hemolysis-related genes derived from bacteria through horizontal gene transfer (HGT) may contribute to virulence in P. persalinus. Hemorrhagic lesions are a common feature of scuticociliatosis, but it is not known whether other scuticociliates also have bacteria-derived hemolysis-related genes. In this study, we constructed a high-quality macronuclear genome of another typical pathogenic scuticociliate, U. marinum. A total of 105 HGT genes were identified in this species, of which 35 were homologs of hemolysis-related genes (including hemolysin-like genes) that had previously been identified in P. persalinus. Sequencing of an additional five species from four scuticociliate families showed that bacteria-derived hemolysis-related genes (especially hemolysin-like genes) are widely distributed in scuticociliates. Based on these findings, we suggest that hemolysin-like genes may have originated before the divergence of scuticociliates.
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Albesa, Inés, Lucila Isabel Barberis, María Cristina Pajaro, María Cecilia Farnochi, and Alberto Jorge Eraso. "A thiol-activated hemolysin in Gram-negative bacteria." Canadian Journal of Microbiology 31, no. 3 (March 1, 1985): 297–300. http://dx.doi.org/10.1139/m85-055.

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An investigation of Klebsiella pneumoniae hemolytic activity was carried out. Strains isolated from different infected specimens were hemolytic in tryptic soy agar with rabbit blood; incubation at 4 °C enhanced the hemolysis. There was no evident red blood cell lysis in plates with human, sheep, mouse, and chicken erythrocytes. The culture in tryptic soy broth, its supernatant and bacterial lysate did produce evident hemolysis of rabbit red blood cells when they were preincubated with 2-mercaptoethanol. Klebsiella pneumoniae hemolysin showed the Arrhenius effect, while temperatures over 60 °C for 10 min reduced the activity of crude hemolysin to zero; purified hemolysin, however, was heat stable. Two hemolysins active on rabbit red blood cells were purified and both shared several properties. This work represents the initial description of a thiol-activated hemolysin in Gram-negative bacteria.
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Yoh, Myonsun, Takeshi Honda, and Toshio Miwatani. "Comparison of hemolysins of Vibrio cholerae non-O1 and Vibrio hollisae with thermostable direct hemolysin of Vibrio parahaemolyticus." Canadian Journal of Microbiology 34, no. 12 (December 1, 1988): 1321–24. http://dx.doi.org/10.1139/m88-231.

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Hemolysin (Vh-rTDH) produced by Vibrio hollisae and hemolysin (NAG-rTDH) produced by Vibrio cholerae non-O1 were characterized and compared with hemolysin (Vp-TDH) produced by Vibrio parahaemolyticus. These three hemolysins are each composed of two subunits and have similar, but not identical, molecular weights. The amino acid compositions of Vp-TDH and NAG-rTDH are similar, but are different from that of Vh-rTDH. The three hemolysins showed similar lethal toxicities to mice. The effects of temperature on hemolysis and the time dependencies of hemolysis by the three hemolysins were similar. The three were concluded to be immunologically related, but not identical, and to have common and also unique antigenic determinants.
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Maltz, Michele, and Joerg Graf. "The Type II Secretion System Is Essential for Erythrocyte Lysis and Gut Colonization by the Leech Digestive Tract SymbiontAeromonas veronii." Applied and Environmental Microbiology 77, no. 2 (November 19, 2010): 597–603. http://dx.doi.org/10.1128/aem.01621-10.

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ABSTRACTHemolysin and the type II secretion system (T2SS) have been shown to be important for virulence in many pathogens, but very few studies have shown their importance in beneficial microbes. Here, we investigated the importance of the type II secretion pathway in the beneficial digestive-tract association ofAeromonas veroniiand the medicinal leechHirudo verbanaand revealed a critical role for the hemolysis of erythrocytes. A mutant with a miniTn5insertion inexeM, which is involved in forming the inner membrane platform in the T2SS, was isolated by screening mutants for loss of hemolysis on blood agar plates. A hemolysis assay was used to quantify the mutant's deficiency in lysing sheep erythrocytes and revealed a 99.9% decrease compared to the parent strain. The importance of the T2SS in the colonization of the symbiotic host was assessed. Colonization assays revealed that the T2SS is critical for initial colonization of the leech gut. The defect was tied to the loss of hemolysin production by performing a colonization assay with blood containing lysed erythrocytes. This restored the colonization defect in the mutant. Complementation of the mutant using the promoter region andexeMNrevealed that the T2SS is responsible for secreting hemolysin into the extracellular space and that both the T2SS and hemolysin export by the T2SS are critical for initial establishment ofA. veroniiin the leech gut.
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Abdulwahhab, Aqeel M., and Khawlah J. Khalaf. "Effect of cultivation conditions on hemolysin production from clinical isolates of Serratia marcescens." Al-Mustansiriyah Journal of Science 33, no. 1 (March 10, 2022): 6–14. http://dx.doi.org/10.23851/mjs.v33i1.1080.

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Sixty-four isolates were collected from clinical sources in Baghdad and Mosul city. The identification of S. marcescens confirmed by using API 20E and VITEK-2 systems. Twelve isolates of S. marcescens produced hemolysin, which were detected by two ways, qualitative hemolytic assay and quantitative hemolytic assay. The optical density of hemolysin producing isolates at standard condition (37oC, pH=7, 24h) showed that Serratia marcescens (SmU9) isolate gave the highest absorbance of hemolysin production (0.7) but at (25°C, pH=7,24h) the absorbance of hemolysin production turn into (3.0), compared with the absorbance at 571nm for Control without hemolysin (0.060) and completely hemolysis by Triton x-100 (6.0). However, the optimum optical density of the best hemolysin producing isolate (SmU9) at the optimum cultivation conditions (25°C, pH=9, 24h, 150 rpm, 1% inoculum size in Nutrient broth) was (6.0).
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Dorrah, Moataza, Chaima Bensaoud, Amr A. Mohamed, Daniel Sojka, Taha T. M. Bassal, and Michail Kotsyfakis. "Comparison of the hemolysis machinery in two evolutionarily distant blood-feeding arthropod vectors of human diseases." PLOS Neglected Tropical Diseases 15, no. 2 (February 4, 2021): e0009151. http://dx.doi.org/10.1371/journal.pntd.0009151.

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Host blood protein digestion plays a pivotal role in the ontogeny and reproduction of hematophagous vectors. The gut of hematophagous arthropods stores and slowly digests host blood and represents the primary gateway for transmitted pathogens. The initial step in blood degradation is induced lysis of host red blood cells (hemolysis), which releases hemoglobin for subsequent processing by digestive proteolytic enzymes. The activity cycles and characteristics of hemolysis in vectors are poorly understood. Hence, we investigated hemolysis in two evolutionarily distant blood-feeding arthropods: The mosquito Culex pipiens and the soft tick Argas persicus, both of which are important human and veterinary disease vectors. Hemolysis in both species was cyclical after blood meal ingestion. Maximum digestion occurs under slightly alkaline conditions in females. Hemolytic activity appears to be of lipoid origin in C. pipiens and enzymatic activity (proteolytic) in A. persicus. We have assessed the effect of pH, incubation time, and temperature on hemolytic activity and the hemolysin. The susceptibility of red blood cells from different hosts to the hemolysin and the effect of metabolic inhibition of hemolytic activity were assessed. We conclude that in C. pipiens and A. persicus midgut hemolysins control the amplitude of blood lysis step to guarantee an efficient blood digestion.
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Söderström, Carl Martin, Steen K. Fagerberg, Mette B. Brogaard, Jens Leipziger, Marianne Skals, and Helle A. Praetorius. "Loop Diuretics Diminish Hemolysis Induced by α-Hemolysin from Escherichia coli." Journal of Membrane Biology 250, no. 3 (May 9, 2017): 301–13. http://dx.doi.org/10.1007/s00232-017-9963-0.

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Bi, Shuai, Wei Wang, Yuanyuan Zhao, Shaoguo Ru, and Yunzhang Liu. "Studies on hemolysis of hemolysin produced by Synechocystis sp. PCC 6803." Journal of Ocean University of China 10, no. 4 (November 6, 2011): 362–68. http://dx.doi.org/10.1007/s11802-011-1823-4.

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Saffioti, Nicolas A., Natalia Lauri, Lucia Cané, Rodolfo Gonzalez-Lebrero, Karina Alleva, Isabelle Mouro-Chanteloup, Mariano A. Ostuni, Vanesa Herlax, and Pablo Julio Schwarzbaum. "Interactive Dynamics of Cell Volume and Cell Death in Human Erythrocytes Exposed to α-Hemolysin from Escherichia coli." International Journal of Molecular Sciences 23, no. 2 (January 14, 2022): 872. http://dx.doi.org/10.3390/ijms23020872.

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α-hemolysin (HlyA) of E. coli binds irreversibly to human erythrocytes and induces cell swelling, ultimately leading to hemolysis. We characterized the mechanism involved in water transport induced by HlyA and analyzed how swelling and hemolysis might be coupled. Osmotic water permeability (Pf) was assessed by stopped-flow light scattering. Preincubation with HlyA strongly reduced Pf in control- and aquaporin 1-null red blood cells, although the relative Pf decrease was similar in both cell types. The dynamics of cell volume and hemolysis on RBCs was assessed by electrical impedance, light dispersion and hemoglobin release. Results show that HlyA induced erythrocyte swelling, which is enhanced by purinergic signaling, and is coupled to osmotic hemolysis. We propose a mathematical model of HlyA activity where the kinetics of cell volume and hemolysis in human erythrocytes depend on the flux of osmolytes across the membrane, and on the maximum volume that these cells can tolerate. Our results provide new insights for understanding signaling and cytotoxicity mediated by HlyA in erythrocytes.
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Dissertations / Theses on the topic "Hemolys"

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Sarras, Marcella. "Påverkan av hemolys vid analys av neuronspecifikt enolas på Cobas." Thesis, Malmö universitet, Fakulteten för hälsa och samhälle (HS), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-44406.

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Neuronspecifikt enolas (NSE) är en viktig biomarkör för att diagnostisera t.ex. neuroendokrina tumörer, särskilt småcellig lungcancer (SCLC) och neuroblastom. NSE används även som en del i utredning av hjärnskada vid hjärtstopp. Eftersom NSE finns i höga koncentrationer i erytrocyter, kan hemolys i blodprovet orsaka falskt förhöjda NSE-nivåer i serum utan hjärnskada. Syftet med studien var att utvärdera hur hemolys påverkar NSE-analysen på Cobas, ett helautomatiserat analysinstrument. Mätning av NSE-koncentration utfördes på Cobas 8000 från Roche Elecsys, baserad på immunokemisk sandwich-metod med ElectroChemi-LuminiscenceImmunoassay (ECLI) detektionsteknik. För att studera hemolysens inverkan, tillverkades hemolysat från 20 patientprover. Dessa hemolysat tillsattes till poolat serum, med NSE-nivåer inom referensintervallet (< 17 µg/L). Även graden av hemolys bestämdes på Cobas 8000. Resultatet visade ett linjärt samband mellan de uppmäta hemolysindex (HI) värden och S-NSE värden. Variationen i NSE-tillskott på individnivå undersöktes och resulterade i slutsatsen att varje hemolysenhet motsvarar ett NSE-tillskott på 0,33 ± 0,07 µg/L som frigörs från erytrocyter. Ett förslag för att lösa problemet med hemolys vid analys av S-NSE är att använda en kompenserande faktor för att korrigera NSE-koncentrationen. Kompensering kan utföras med hjälp av det erhållna sambandet i studien (1 HI = 0,33 ± 0,07 µg/L NSE-tillskott) genom att subtrahera tillskottet från den uppmätta NSE-koncentrationen.
Neuron Specific Enolase (NSE) is an important biomarker for diagnosing e.g. neuroendocrine tumors, especially small cell lung cancer (SCLC) and neuroblastoma. NSE is also used as a part of the investigation of brain damage in cardiac arrest. Because NSE is present in high concentrations in erythrocytes, hemolysis in the blood sample can cause falsely elevated NSE levels in serum without brain damage. The purpose of this study was to evaluate how hemolysis affects NSE analysis on Cobas, a fully automated analytical instrument. Measurement of NSE concentration was performed on Cobas 8000 from Roche Elecsys, based on immunochemical sandwich method with ElectroChemi-Luminescence Immunoassay (ECLI) detection technique. To study the effect of hemolysis, hemolysates were prepared from 20 patient samples. These hemolysates were added to pooled serum, with NSE levels within the reference range (<17 μg/L). The degree of hemolysis was also determined on Cobas 8000. The result showed a linear relationship between the measured hemolysis index (HI) values and S-NSE values. The variation in NSE contribution at the individual level was examined with the result that each hemolysis unit corresponds to an NSE contribution of 0.33 ± 0.07 µg/L, which is released from erythrocytes. A suggestion to solve the problem of hemolysis relating to NSE analysis is to use a compensatory factor to correct the NSE concentration. Compensation can be performed by using the relationship obtained in the study (1 HI = 0.33 ± 0.07 µg/L NSE contribution) and subtracting the contribution from the measured NSE concentration.
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Saleh, Amal. "Interferens genom hemolys som påverkar analys av S- bilirubin på Olympus AU2700." Thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-30444.

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Felaktig provtagning kan orsaka hemolys som kan påverka mätresultatet av S-bilirubin koncentrationen vid användning av diazometoder. Bilirubin är ett gulfärgat pigment som erhålls när hemolglobin bryts ner. Vätebindningar som finns i bilirubinstrukturen stabiliserar molekylen och avgör om den är löslig eller inte löslig i vatten. Bilirubin konjugeras i levern och blir vattenlösligt, därefter utsöndras bilirubin via gallan och tarmen. Hyperbilirubinemi uppstår vid ökad nedbrytning av erytrocyter eller vid leverskada. Ökad bilirubinkoncentration i blodet sker ofta hos nyfödda och diffunderar till kroppens celler och orsakar icterus. Studien gjordes med syftet att studera hur olika grad av hemolys påverkar analys av S-bilirubin-koncentrationen. S-bilirubinkoncentration analyserades kolormetrisk i Olympus AU2700. Diazoreaktionen sker mellan bilirubin och diazotiserad sulfanilsyra som bildar en färgad produkt, azobilirubin. Azobilirubin analyseras genom absorbansmätning vid 540 nm. Absorbansen är proportionell mot totala bilirubinkoncentrationen. En hemolysat-spädningslösning gjordes och användes som en spädningsslösning till 16 stycken olika serumprover för att få olika hemolysgrader 0 g/L, 0,5 g/L, 1 g/L, 3 g/L respektive 5 g/L. Resultatet visade att hemolys sänkte S-bilirubinkoncentrationen vid mätning i instrumentet som använder diazimetoder. Vid högre grad av hemolys som tillsattes till S-bilirubinkoncentrationen desto lägre blev S-bilirubinkoncentrationen som analyserades i proverna. Alla prover som hade låg S-bilirubinkoncentration hade större avvikelser jämfört med de prover som hade hög S-bilirubinkoncentration. Vid tillsats av hemolys med 0,5 g/L till prover med S-bilirubinkoncentrationen under 20 µmol/L låg avvikelsen mellan 12,00 % och 16,00 % medan vid tillsats av samma hemolysgrad till S-bilirubinkoncentrationen över 20 µmol/L låg avvikelsen mellan 0,67 % och 4,00 %. Slutsatsen är att patientprover som har S-bilirubinkoncentrationer lägre än 20 µmol/L och med hemolys inte kan mätas, prover från patienter med hyperbilirubinemi och hemolysgrad lägre än 1 g/L kan analyseras i de fall de är svåra att ta om t.ex på nyfödda.
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Frohm, Hanna. "Titerbestämning av anti-A och anti-B i trombocytenheter för transfusion över ABO gränsen : utvärdering av rutinanalys och utveckling av en screeningmetod." Thesis, Högskolan Kristianstad, Fakulteten för naturvetenskap, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-18623.

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Trombocyter är suspenderade i plasma som innehåller antikroppar mot de blodgruppsantigen som saknas på erytrocyterna. För att minimera risken för en hemolytisk reaktion bestäms titern av anti-A och anti-B. Gelkortsteknik används för att detektera antikropp-antigensreaktioner och baseras på agglutinationer i en gel. Syftet med studien var att undersöka titern av anti-A och anti-B i trombocytenheter, samt att utvärdera en rutinanalys och utveckla en screeningmetod. I studien analyserades enheter av blodgrupp O och A. De kontrollerades mot anti-A och/eller anti-B både för IgG och IgM antikroppar. En screeningmetod utvecklades för att kunna screena O-enheterna och en gräns på 1:100 respektive 1:250 undersöktes. Resultatet kunde påvisa en stor skillnad i titer mellan O-och A-enheter. Titern skiljer sig signifikant beroende på om titern bestäms i plasma eller från den färdiga (utspädda) enheten. En screeningmetod på 1:100 påvisade att 86 % av enheterna hade bedömts som hög titer och en screeningmetod på 1:250 visade att andelen sjönk till 31 %. Geltekniken är en känslig metod och är beroende av kompetent personal vid avläsning. En del studier visar liknande resultat men andelen enheter med hög titer varierar och likaså metoder och titergräns. Detta påvisar svårigheterna i att bestämma en kritisk titer och att förutse risker hos patienten. Andra faktorer tros också kunna påverka riskerna. Införande av en screeningmetod på 1:250 kan öka antalet enheter som kan transfunderas över ABO-barriären.
Platelets are suspended in plasma containing antibodies to the blood group antigen missing on the erythrocytes. To minimize the risk of hemolytic reaction, the titrers of anti-A and anti-B are determined. The gel test is used to detect antibody-and antigen responses and is based on agglutinations in gel. The purpose was to investigate the titers of anti-A and/or anti-B in platelets. A routine analysis was evaluated and a screening method was implemented. In the study, units of blood group O and A were analyzed. They were checked against anti-A and anti-B for both IgG and IgM antibodies. A screening method was developed to screen the O-units and a limit of 1:100 and 1:250 was used. The results showed great difference in titers between O and A units. The titers differ significantly depending on whether the titers are determined in plasma or from the finished (diluted) unit. A screening method at 1:100 showed that 86 % of the units was rated as high titer while a screening method of 1:250 showed that this was reduced to 31 %. Gel technology is a sensitive method and is dependent on competent staff when reading the agglutinations. Some studies show similar results, but the proportion of high titer units, methods and critical titers varies. It proves the difficulty in determining a critical titer and predicting risks for the patient. Other factors are also believed to influence the risks. Implementation of a 1:250 screening method is believed to increase the number of units that can be transfused over the ABO barrier.
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Chornogorets, Valeriya, and Валерія Чорногорець. "Study of genetic features of blood groups heredity according to the AB0 system and rhesus factor." Thesis, National Aviation University, 2021. https://er.nau.edu.ua/handle/NAU/50772.

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1. Рагимов А.А., Дашкова Н.Г. Основы трансфузионной иммунологии. М., 2004. С. 68. 2. Blood group terminology 2004: from the International Society of Blood Transfusion committee on terminology for red cell surface antigens / G. Daniels et al. Vox Sang. 2004. № 87. Р. 304-316. 3. Yamamoto F., Yamamoto M. Molecular genetic basis of porcine histo-blood group ABO system. Blood. 2001. Vol. 97, № 10. P. 3308–3310. 4. Лавряшина М.Б., Толочко Т.А., Волков А.Н. Аллоантигены крови человека: учеб. пособ. Кемерово: Кузбассвузиздат, 2006. 100 с. 5. Практическая трансфузиология / под ред. Г.И. Козинца. М.: Практическая медицина, 2005. 544 с.
The work is devoted to the problem of blood transfusion (namely, the study of the most common blood group and rhesus factor in countries), because this issue has become acute around the world. Today, blood transfusions are an acute social problem. Two classifications of human blood groups are of the greatest importance for clinical practice: the AB0 system and the rhesus system, due to the fact that these systems have the greatest antigenic power. Each human-to-human blood transfusion must take into account the compatibility of these two systems, because in the case of a human transfusion of another (incompatible) blood group, agglutination (gluing) and hemolysis (destruction) of erythrocytes occur, which can lead to death. It is also desirable to separate plasma and blood cells.
Робота присвячена проблемі переливання крові (а саме вивченню найпоширенішої групи крові та резус-фактора в країнах), оскільки ця проблема загострилася у всьому світі. Сьогодні переливання крові є гострою соціальною проблемою. Дві класифікації груп крові людини мають найбільше значення для клінічної практики: система AB0 та резус-система через те, що ці системи мають найбільшу антигенну силу. При кожному переливанні крові людині необхідно враховувати сумісність цих двох систем, оскільки у випадку переливання людиною іншої (несумісної) групи крові відбувається аглютинація (склеювання) та гемоліз (руйнування) еритроцитів, що може привести до смерті. Також бажано відокремити плазму та клітини крові.
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Menegazzo, Ana Barbara Bordignon Rodrigues. "Determinação do valor da heptoglobina sérica para diagnóstico de hemólise na síndrome HELLP /." Botucatu, 2014. http://hdl.handle.net/11449/113896.

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Orientador: Joelcio Francisco Abbade
Banca: José Carlos Peraçoli
Banca: Francisco Lázaro Pereira de Souza
Resumo: INTRODUÇÃO: A síndrome HELLP é uma complicação severa da pré-eclâmpsia, caracterizada por hemólise, elevação das enzimas hepáticas e trombocitopenia. Apesar de haver padronização dos valores laboratoriais que definem a síndrome HELLP, ainda existe dificuldade para a caracterização da hemólise. OBJETIVO: Avaliar o valor de haptoglobina que determina a hemólise nas pacientes com síndrome HELLP. MÉTODOS: estudo transversal e prospectivo de gestantes e puérperas com de pré-eclâmpsia. Exame laboratorial avaliado: dosagem sérica de haptoglobina. Construção da curva ROC para determinar o valor de corte da haptoglobina para diagnóstico de hemólise na síndrome HELLP. RESULTADOS: O valor da haptoglobina para diagnóstico de hemólise em pacientes com síndrome HELLP foi de 0,26g/L. DISCUSSÃO: A melhor correlação observada foi a haptoglobina com a DHL, indicando que este é o melhor marcador de hemólise intravascular para o diagnóstico da síndrome HELLP. CONCLUSÃO: A dosagem sérica da haptoglobina nos casos de pré-eclâmpsia deve fazer parte dos exames de rotina para diagnóstico de hemólise intravascular da síndrome HELLP
Abstract: CONTEXT: HELLP syndrome is a severe complication of pre-eclampsia, caracterized by hemolysis, elevated liver enzymes and low platelet count. Although there are standardized laboratory values that define the HELLP syndrome, the difficulty still exists for the characterization of hemolysis. PURPOSE: to evaluate the haptoglobin value to diagnose the hemolysis in HELLP syndrome. METHODS: transversal and prospective study of pregnant and postdelivery women with pre-eclampsia. Laboratory tests evaluated: serum haptoglobin. ROC curve to determine the cutoff value of haptoglobin in the diagnosis of hemolysis in HELLP syndrome. RESULTS: The haptoglobin value for hemolysis diagnosis in HELLP syndrome was 0.26 g / L. DISCUSSION: The best correlation was with haptoglobin and DHL, indicating that this is the best marker of intravascular hemolysis for the diagnosis of HELLP syndrome. CONCLUSION: Serum haptoglobin in cases of pre-eclampsia should be part of routine tests for diagnosis of intravascular hemolysis in HELLP syndrome
Mestre
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6

Baltyde, Kizzy-Clara. "Implication de la voie adénosine/adénosine récepteur A2B dans les mécanismes physiopathologiques de deux manifestations drépanocytaires : l'hémolyse et le priapisme." Thesis, Antilles, 2016. http://www.theses.fr/2016ANTI0039/document.

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La drépanocytose résulte d’une mutation du gène β-globine entrainant la synthèse d’une hémoglobine anormale, l’HBS, qui polymérise en condition désoxygénée. Le globule rouge deviendra plus rigide et plus fragile, donnant lieu a deux conséquences majeures : l’hémolyse accrue et l’occlusion vasculaire.Récemment, un nouvel acteur moléculaire, l’adénosine, a été identifie. Ce nucléoside présente des effets bénéfiques sur les atteintes pulmonaires en inhibant l’activation des cellules « tueur naturel t inductibles », mais aussi délétères en favorisant la polymérisation de l’HBS et la survenue du priapisme, une des complications drépanocytaires.Les travaux menés ont pour objectifs d’étudier les effets délétères potentiels de l’adénosine, à travers l’étude de l’implication des enzymes de la voie métabolique de l’adénosine dans l’hémolyse ainsi que dans la survenue du priapisme chez des patients drépanocytaires. Pour ce faire, une cohorte composée d’adultes et d’enfants SS a été étudiée. Les résultats obtenus ont permis de préciser le rôle du métabolisme de l’adénosine dans les mécanismes physiopathologiques de la drépanocytose. Nos résultats n’ont pas permis de mettre en évidence de différence d’expression (ARN, protéine) des enzymes du métabolisme de l’adénosine entre les patients présentant des antécédents priapiques et ceux indemnes de cette complication. Néanmoins, nos recherches ont permis d’identifier l’adénylate cyclasse 6 comme gène modulateur de l’hémolyse et d’apporter de nouveaux éléments en accord avec la classification du priapisme comme appartenant au sous-phénotype hyperhémolytique à travers notamment l’étude des caractéristiques hemorhéologiques
Sickle-cell disease is caused by a mutation in the β-globin gene leading to an abnormal hemoglobin, hbs. This change allows polymerization of HBS when deoxygenated. Erythrocytes become more rigid and fragile, leading to the two major manifestations of the disease: hemolysis and vaso-occlusion.Recently, adenosine has been identified as a new molecular actor of this disease. This nucleoside may have beneficial effects by preventing inkt cells activation and pulmonary inflammation. But it may also exhibit deleterious effects by activing a signalling pathway leading to erythrocyte sickling and the occurrence of priapism, a sickle cell disease complication.The purpose of our work, based on the potential deleterious effects induced by adenosine was to precise the involvement of adenosine metabolic pathway enzymes in hemolysis and the occurrence of priapism. Two cohorts of children and adult ss patients and men ss have been studied respectively.Our results had clarified the role of metabolism of adenosine in the pathophysiological mechanisms of sickle cell disease. Our results did not allow detecting evidence of differential expression (rna, protein levels) of adenosine metabolism enzymes between ss adult patients exhibiting priapic events and those who had never experienced this complication. Nevertheless, our work has led to the identification of adenylate cyclase as modifier gene of hemolysis and has bring new elements on the priapism classification to the hyper hemolytic sub-phenotype with the description of the hemorheological features associated with this complication
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Menegazzo, Ana Barbara Bordignon Rodrigues [UNESP]. "Determinação do valor da heptoglobina sérica para diagnóstico de hemólise na síndrome HELLP." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/113896.

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Introdução: A síndrome HELLP é uma complicação severa da pré-eclâmpsia, caracterizada por hemólise, elevação das enzimas hepáticas e trombocitopenia. Apesar de haver padronização dos valores laboratoriais que definem a síndrome HELLP, ainda existe dificuldade para a caracterização da hemólise. OBJETIVO: Avaliar o valor de haptoglobina que determina a hemólise nas pacientes com síndrome HELLP. MÉTODOS: estudo transversal e prospectivo de gestantes e puérperas com de pré-eclâmpsia. Exame laboratorial avaliado: dosagem sérica de haptoglobina. Construção da curva ROC para determinar o valor de corte da haptoglobina para diagnóstico de hemólise na síndrome HELLP. RESULTADOS: O valor da haptoglobina para diagnóstico de hemólise em pacientes com síndrome HELLP foi de 0,26g/L. DISCUSSÃO: A melhor correlação observada foi a haptoglobina com a DHL, indicando que este é o melhor marcador de hemólise intravascular para o diagnóstico da síndrome HELLP. CONCLUSÃO: A dosagem sérica da haptoglobina nos casos de pré-eclâmpsia deve fazer parte dos exames de rotina para diagnóstico de hemólise intravascular da síndrome HELLP
Context: HELLP syndrome is a severe complication of pre-eclampsia, caracterized by hemolysis, elevated liver enzymes and low platelet count. Although there are standardized laboratory values that define the HELLP syndrome, the difficulty still exists for the characterization of hemolysis. PURPOSE: to evaluate the haptoglobin value to diagnose the hemolysis in HELLP syndrome. METHODS: transversal and prospective study of pregnant and postdelivery women with pre-eclampsia. Laboratory tests evaluated: serum haptoglobin. ROC curve to determine the cutoff value of haptoglobin in the diagnosis of hemolysis in HELLP syndrome. RESULTS: The haptoglobin value for hemolysis diagnosis in HELLP syndrome was 0.26 g / L. DISCUSSION: The best correlation was with haptoglobin and DHL, indicating that this is the best marker of intravascular hemolysis for the diagnosis of HELLP syndrome. CONCLUSION: Serum haptoglobin in cases of pre-eclampsia should be part of routine tests for diagnosis of intravascular hemolysis in HELLP syndrome
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Thome, Jacqueline Darc Silva. "Citotoxinas e hemolisinas produzidas por Campylobacter jejuni isolados de diferentes origens." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317312.

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Orientador: Tomomassa Yano
Dissertação (mestrado) - Universidade Estadual de Campinas,. Instituto de Biologia
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Mestrado
Microbiologia
Mestre em Genética e Biologia Molecular
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Coussios, Constantin-Cassios. "Monitoring of hemolysis by acoustic scattering." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620292.

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Russell, Laura René. "Treponema hyodysenteriae: growth and production of hemolysin." Thesis, Virginia Tech, 1988. http://hdl.handle.net/10919/45180.

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Treponema hyodysenteriae is the causative agent of swine dysentery, a mucohemorrhagic disease of the intestines. T. hyodysenteriae requires phospholipids and cholesterol (or cholestanol) for growth, and it produces a a-hemolysin (TH) which is induced (300 fold) by the addition of RNA core to cultures. TH is bound to the RNA core which acts as a carrier or stabilizer. I\lyobjectives were to (i) obtain successful continuous growth of T. hyodysenteriae; (ii) study the production of the hemolysin produced by T. hyodysenteriae; (Ui) study the effects of different oligonucleotide carriers on the induction of hemolysin, (iv) examine various purification procedures for nipid, efficient partial purification of the hemolysin, (v) separate the hemolysin from the RNA core carrier, and (vi) to detennme whether T. hyodysenteriae can use coprostanol, the most common intestinal sterol, to satisfy its sterol requirement. I found that optimal growth of T. hyodysenteriae could be achieved by using BHI- glucose broth supplemented with calf serum (I O~/o). serum replacement (100/0), or phosphatidylcholine liposomes which contained cholesterol or cholestanol. Optimal growth required 1 % 02 and stirring of the culture. l\laximal hemolytic titers were obtained during late-log to early-stationary phase by cultures grown in the presence of RNA core. Hemolysin production was induced as soon as 5 minutes after addition of RNA core to cultures. This production was inhibited by chloramphenicol. Polyguanylic acid and RNase treated RNA core did not significantly increase hemolytic titers of cultures grown in their presence. Partial purification of hemolysin was achieved by acetic acid clarification followed by ammonium sulfate precipitation (65% saturation). With these procedures > 90% of the hemolytic activity was recovered. Although, hydroxylapatite adsorption and polyethyleneimine precipitation completely adsorbed or precipitated hemolytic activity I I was unable to efficiently recover the activity. Partially purified hemolysin was c}10toxic to CHO cells, and caused lysis rather than the rounding effect caused by many cytotoxins.
Master of Science
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Books on the topic "Hemolys"

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Menem), Argentina President (1989 :. Hemos cambiado la historia. [Buenos Aires, Argentina]: Presidencia de la Nación, Secretaría de Medios de Comunicación, 1994.

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Campos, Marco Antonio. Hemos perdido el reino. México: J. Mortiz, 1987.

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Meneses, Enrique. Hasta aquí hemos llegado. La Coruña [Spain]: Ediciones del Viento, 2006.

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Alabau, Magaly. Hemos llegado a Ilion. Madrid: Betania, 1992.

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Uribe, Willy. Los que hemos amado. Barcelona: Libros del Lince, 2011.

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Las cosas que hemos visto. Sevilla: Renacimiento, 2011.

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Hemos caminado siglos esta madrugada. Panamá: Instituto Nacional de Cultura, 2011.

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Los días que hemos visto. [Valladolid]: Fundación Jorge Guillén, 2009.

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Bifet, Juan Esquerda. Te hemos seguido: Espiritualidad sacerdotal. 2nd ed. Madrid: Biblioteca de Autores Cristianos, 1988.

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Tébar, Juan. Las cosas que hemos visto. Madrid: Nickel Odeon, 1993.

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Book chapters on the topic "Hemolys"

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Becker, Katja, and R. Heiner Schirmer. "THE EGRAC AS A MEASURE OF THE RIBOFLAVIN STATUS IN MAN. TITRATION OF HEMOLYS ATE FAD WITH APOGLUTATHIONE REDUCTASE." In Flavins and Flavoproteins 1990, edited by B. Curti, S. Ronchi, and G. Zanetti, 851–54. Berlin, Boston: De Gruyter, 1991. http://dx.doi.org/10.1515/9783110855425-165.

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Gooch, Jan W. "Hemolysin." In Encyclopedic Dictionary of Polymers, 897. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13895.

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Gooch, Jan W. "Hemolysis." In Encyclopedic Dictionary of Polymers, 897. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13896.

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Anderson, David R., and John G. Kelton. "Hemolysis and Thrombocytopenia." In The Complete Spleen, 193–212. Totowa, NJ: Humana Press, 2002. http://dx.doi.org/10.1007/978-1-59259-124-4_12.

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Gooch, Jan W. "Alpha (α) Hemolysin." In Encyclopedic Dictionary of Polymers, 873. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13097.

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Gooch, Jan W. "Alpha (α) Hemolysis." In Encyclopedic Dictionary of Polymers, 873. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13098.

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Gooch, Jan W. "Beta (β) Hemolysin." In Encyclopedic Dictionary of Polymers, 878. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13246.

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Gooch, Jan W. "Beta (β) Hemolysis." In Encyclopedic Dictionary of Polymers, 878. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13247.

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Ridder, Miranda J., Seth M. Daly, Pamela R. Hall, and Jeffrey L. Bose. "Quantitative Hemolysis Assays." In Methods in Molecular Biology, 25–30. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1550-8_4.

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Mijovic, Aleksandar. "Dial M for HeMolysis." In Transfusion Medicine, 31–34. London: Springer London, 2011. http://dx.doi.org/10.1007/978-1-4471-2182-4_8.

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Conference papers on the topic "Hemolys"

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Kim, Gi-Beum, Mun-Yong Lee, Seol-Hee Jeon, Md Mizanur Rahman, Min-Ho Kim, Seong-Jong Kim, In-Shick Kim, Jin-Shang Kim, Hyung-Sub Kang, and Chul-Un Hong. "Blood Hemolysis of Implantable Artificial Lung." In 2008 International Conference on Biomedical Engineering And Informatics (BMEI). IEEE, 2008. http://dx.doi.org/10.1109/bmei.2008.105.

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Tacheva, Bilyana, Boyana Paarvanova, Stanislav Bozhikov, Ivan T. Ivanov, and Miroslav Karabaliev. "Kinetics of hemolysis induced by thioridazine." In PROCEEDINGS OF THE INTERNATIONAL CONFERENCE OF COMPUTATIONAL METHODS IN SCIENCES AND ENGINEERING 2019 (ICCMSE-2019). AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5138026.

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Dasi, Lakshmi Prasad, Khandakar Niaz Morshed, and Marcio Forleo. "Phenomenology of Hemolysis in Turbulent Flows." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14843.

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Shear induced hemolysis (SIH) refers to the rupture of red blood cells in shear flows resulting in the release of hemoglobin into plasma. Hemolysis in turn triggers a chain of bio-chemical events involving all the components of blood leading to the formation of thrombosis (blood clots). While SIH is negligible in a healthy circulatory system, it is widespread in life saving blood contacting devices such as heart valves, ventricular assist devices, and blood pumps. Thus an accurate physical understanding of the process of hemolysis is critical towards developing better predictive models of SIH, particularly in the context of engineering future blood contacting devices that are significantly more blood cell friendly.
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Timlin, Homa. "161 Low haptoglobin level in lupus without hemolysis." In 13th International Congress on Systemic Lupus Erythematosus (LUPUS 2019), San Francisco, California, USA, April 5–8, 2019, Abstract Presentations. Lupus Foundation of America, 2019. http://dx.doi.org/10.1136/lupus-2019-lsm.161.

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Farooq, M. F., and P. Grover. "Clostridium Perfringens Liver Abscess with Massive Intravascular Hemolysis." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a3549.

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Capata, Roberto. "Peristaltic Roller Pump: Parametric Optimization for Hemolysis Control." In ASME 2020 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/imece2020-23371.

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Abstract This work aims to analyze the various types of peristaltic pumps by studying, in particular, the use of the peristaltic roller pump to highlight its critical issues and propose new effective and innovative solutions. One possible application of this device is in hemodialysis, which is a physical therapy substitution of kidney function that allows, in almost all cases, recovery and maintenance of the main biological functions while remaining the uremic condition. As for the extracorporeal one, the equipment used to purify the blood from toxic substances that are no longer normally eliminated by kidney filtration is divided mainly into two types: rotary peristaltic pump and a linear peristaltic pump. Having to work with a very particular fluid such as blood and in direct contact with the patient, they need to be extremely accurate and must ensure a constant and continuous functioning. The rotary peristaltic pump is the most widely used for hemodialysis and having been extensively studied in literature it has since found extensive solutions in the application field. As is well known, peristaltic pump refers to a device that exploits the principle of peristalsis to function, i.e. the transit of a bottleneck on a tube, in this case, the catheter, to push the fluid contained outwards. In particular, a roundabout peristaltic pump consists of a rotating structure consisting of two or more rollers that in turn revolve around their axis. With their displacement, the rollers clog adjacent catheter sections at a time so that after the first roller has passed the tube returns to its initial size creating the vacuum and then sucking the fluid. In this way, the liquid is pushed from the tube towards the patient. The motion of all these components is powered by an electric motor connected directly to the main rotating structure. The pumping of fluids through hoses using the propagation of a peristaltic wave has been the subject of design and scientific studies for more than 4 decades. This is easily justifiable since the phenomenon of peristalsis is known to be an important responsible mechanism of fluid transport in many biological organs. The goal is (starting on studies on the blood, a variable density fluid) to analyze in detail the peristaltic roller pump and propose its parametric optimization, aimed at determining the critical speed, beyond which the machine damages any kind of fluid that needs special treatment (blood, food, special gel, medical ointments and so on).
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Wang, Shijin, Cheng Chen, Johannes Mueller, and Sebastian Mueller. "Evidence for alcohol-mediated masked hemolysis in ALD patients and a vicious cycle of hemolysis and iron-mediated liver damage." In 38. Jahrestagung der Deutsche Arbeitsgemeinschaft zum Studium der Leber. Georg Thieme Verlag, 2022. http://dx.doi.org/10.1055/s-0041-1740745.

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Ikehara, Yuzuru, Sanae Ikehara, Hajime Sakakita, Takashi Yamaguchi, Jaeho Kim, Masahiro Yamagishi, Yoshihiro Akimoto, et al. "Plasmatreatment induces blood clot formation; protein aggregation and hemolysis." In 2016 IEEE International Conference on Plasma Science (ICOPS). IEEE, 2016. http://dx.doi.org/10.1109/plasma.2016.7534074.

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Bessmeltsev, Stanislav S., Alexander V. Lendiaev, Yulia A. Skvortsova, and Vladimir A. Tarlykov. "Laser diffractometry of erythrocyte deformation under hypo-osmotic hemolysis." In International Conference on Lasers for Measurements and Information Transfer, edited by Vadim E. Privalov. SPIE, 2000. http://dx.doi.org/10.1117/12.407659.

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Jahanpanah, Newsha, Sneha Sharma, Karla P. Mercado-Shekhar, Haili Su, Hunter A. Palcich, Austin M. Wanek, and Kevin J. Haworth. "Hemolysis resulting from acoustic droplet vaporization and inertial cavitation." In 2019 International Congress on Ultrasonics. ASA, 2019. http://dx.doi.org/10.1121/2.0001240.

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Reports on the topic "Hemolys"

1

Passariello, Fausto. Hemodyn 2015. Fondazione Vasculab, 2015. http://dx.doi.org/10.24019/2015.hemodyn.

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Durán Toledo, Diego. Los peligros del ministro candidato. Universidad Autónoma de Chile, July 2021. http://dx.doi.org/10.32457/20.500.12728/90532021110.

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Durante la última semana hemos visto que la carrera por llegar al sillón de La Moneda ha sumado un nuevo posible candidato, debido a la intención de Evopoli de transformar al titular de Hacienda, Ignacio Briones, en su carta presidencial.
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Garcimartín, Carlos, Jhonatan A. Astudillo, and Christian Schneider. Inclusión financiera en Panamá. La importancia de la calidad institucional y las brechas territoriales, de ingreso y de género. Inter-American Development Bank, May 2022. http://dx.doi.org/10.18235/0004207.

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La inclusión financiera ha venido adquiriendo un interés creciente, tanto a nivel académico, como por parte de las autoridades, el sector privado y la sociedad civil. Es así porque la inclusión financiera favorece el desarrollo económico, la reducción de la pobreza, el aumento de la equidad y ayuda a cerrar las brechas de género. El primer objetivo de este trabajo es elaborar un índice de inclusión financiera que permita analizar la situación relativa de Panamá. Los resultados muestran que la inclusión financiera de los hogares panameños es relativamente menor a lo que cabría esperar, dado el nivel de desarrollo alcanzado por el país. En segundo lugar, hemos estimado el índice por grupos sociales, encontrando que existe un sesgo de género, de nivel de ingresos y en contra de las áreas rurales en la inclusión financiera. Posteriormente, hemos analizado los factores que explican que la relativamente baja inclusión financiera en Panamá. De acuerdo con nuestros resultados, las principales causas son el peso relativo del empleo en la agricultura, la desigualdad de ingresos, la brecha de género, la calidad institucional y los desequilibrios territoriales que existen en el país; factores que remiten a desafíos estructurales que enfrenta Panamá. Por último, hemos analizado la inclusión financiera digital, encontrando que, al igual que sucede con la general, los resultados de Panamá son inferiores a lo que se esperaría, dado su nivel de desarrollo, siendo las causas similares a las anteriores, junto al bajo nivel educativo promedio y el escaso desarrollo de las industrias fintech. Todo ello pone de manifiesto que aumentar la inclusión financiera pasa por seguir mejorando en esos desafíos estructurales del país: equidad de género, distribución del ingreso, calidad institucional, equilibrio territorial y educación, además de las fintech en el caso digital. Panamá ha realizado avances en estos ámbitos a lo largo de los últimos años. Seguir progresando permitirá la inclusión financiera de un mayor número de panameños y, especialmente, de panameñas que aún no tienen acceso a los servicios financieros, lo cual, a su vez, contribuirá no solo al crecimiento económico, sino, además, a que éste sea más inclusivo.
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Comunicación de las Ciencias, Centro. Mentalidad positiva. Universidad Autónoma de Chile, April 2020. http://dx.doi.org/10.32457/2050012728/9611202045.

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La actual situación epidemiológica por la que atravesamos representa un escenario nuevo para la sociedad. No solo para los encargados de tomar decisiones, sino también para personas comunes que -en un tiempo acotado- nos hemos visto bombardeados de información dónde se nos enseñan cosas simples; desde reaprender a lavarnos las manos, hasta complejidades como entender qué material elegir para poder confeccionar una mascarilla casera. Sin embargo, dentro de este pool de información recibida, es difícil discernir e identificar la que es verdadera y confiable, más aún con las tecnologías actuales y las redes sociales.
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Reséndiz, Diana, and Áurea Esquivel, eds. Almanaque extraordinario de experiencias comunitarias. Chair Magdala López Ramírez. Universidad Nacional Autónoma de México, Centro Cultural Universitario Tlatelolco, 2021. http://dx.doi.org/10.22201/ccut.001r.2021.

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Almanaque: Del árabe المناخ)pr.al-manakh) ‘calendario’, derivado del árabe “munakh” que significa ‘parada de un viaje’, ‘descanso’. Es aquí donde nos proponemos hacer una pausa para revisitar el camino recorrido y reunir las experiencias que al paso del tiempo hemos atesorado como extraordinarias. Estás por recorrer un proyecto editorial colaborativo que celebra los primeros diez años de la Unidad de Vinculación Artística (UVA). Diez años que se expresan en 10 fanzines o números donde cada uno representa una arista desde donde mirar esta primera década. Ubicada en el corazón de Tlatelolco, la UVA ha acompañado en sus procesos de iniciación, formación y creación artística a más de 10,000 personas. A través de nuestros talleres libres y nuestra adorada biblioteca comunitaria “Alaíde Foppa” hemos sido cómplices de niñas, niños, jóvenes, adultos y personas mayores. Para ellxs, y para los que están por venir, nuestro total agradecimiento. Doce talleristas —artistas de diversas disciplinas, interesados en la educación e investigación a través del arte— acudieron al llamado para dimensionar y proyectar nuestra historia. A través de sus voces se hacen evidentes los pilares de nuestra comunidad: la educación no formal, el reconocimiento del otro como vértice de la experiencia educativa y por último, la integración de la carga cultural que representa el barrio de Tlatelolco: nuestro territorio. Así pues, sólo nos queda darte la bienvenida y desear que este descanso de tu propio viaje sea una pausa gozosa y te permita –como nos permitió a nosotras– imaginar nuevos horizontes.
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Mujica, Serrana, and Ana Castillo. 5 iniciativas para favorecer la equidad de género en las empresas. Inter-American Development Bank, February 2021. http://dx.doi.org/10.18235/0003059.

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Hace años venimos hablando de las brechas de género en el mercado laboral, pero ¿qué tanto hemos avanzado? ¿Qué iniciativas podemos implementar rápidamente para reducir esa distancia? La desigualdad de género es un problema estructural en nuestra región. Por eso, el Grupo BID, PwC y ONU Mujeres, en el marco del programa Ganar-Ganar, decidieron unir fuerzas para promover distintas acciones que favorezcan la equidad a través del premio anual dirigido a la empresas llamado "El talento no tiene género". En este documento, se recopilan lecciones aprendidas y buenas prácticas de las experiencias de algunos participantes de la edición 2019.
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Astorga, Ignacio, Paloma Alonso, Diana M. Pinto, Jazmin Freddi, and Martín Corredera Silván. 10 años de Asociaciones Público-Privadas (APP) en salud en América Latina: ¿Qué hemos aprendido?: Nota 4 de la serie de notas técnicas sobre asociaciones público-privadas en el sector de la salud en América Latina. Inter-American Development Bank, July 2016. http://dx.doi.org/10.18235/0000356.

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Garcimartín, Carlos, Carolina Freire, and Jhonatan Astudillo. Impacto social de la pandemia del Covid-19 en Panamá y análisis de eficiencia de los programas de transferencias monetarias. Inter-American Development Bank, July 2021. http://dx.doi.org/10.18235/0003367.

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El primer objetivo de este documento es realizar una estimación del impacto de la crisis de la COVID-19 sobre la pobreza y la desigualdad en Panamá basada en la Encuesta de Mercado Laboral de 2020. Según estas estimaciones, en comparación con 2019, la pobreza general habría aumentado 12,5 puntos y la extrema 6,8. Estos resultados, no obstante, reflejan una suerte de fotografía del momento en que se hizo la encuesta (entre septiembre y octubre de 2020), por lo que a medida que la economía continúe reactivándose estas cifras bajarán. El segundo objetivo es evaluar la contribución a la reducción de la pobreza de los principales programas de transferencias monetarias condicionadas con que cuenta Panamá. Dado que el objetivo de este análisis es de carácter estructural, hemos optado por realizarlo para 2019, evitando la especial coyuntura de 2020. Nuestras estimaciones sugieren que, pese a los avances logrados, el impacto de estos programas en la reducción de la pobreza es relativamente modesto, aunque mayor en cuanto a la brecha de pobreza. Las razones son diversas y, entre ellas, se encuentra su baja cobertura y elevada filtración. Ahora bien, cabe subrayar que conseguir una perfecta focalización podría reducir aún menos la pobreza. En parte, ello se debe a que el proxy actualmente utilizado para determinar la elegibilidad de los beneficiarios a los programas está desactualizado, de manera que un porcentaje relativamente alto de los beneficiarios “filtrados”, aunque no son pobres o pobres extremos según dicho proxy, sí lo son en términos de pobreza monetaria. Esto pone de manifiesto: 1) la disociación que se ha generado con el paso del tiempo entre la pobreza monetaria y la definida por el proxy; 2) lo necesario de avanzar en la implementación del nuevo proxy que ya tiene el país; 3) la importancia de realizar actualizaciones periódicas de los proxys; y 4) lo importante de seguir mejorando en la adecuada certificación de los receptores de los programas.
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Mecanismo Independiente de Consulta e Investigación: informe anual 2021. Inter-American Development Bank, March 2022. http://dx.doi.org/10.18235/0004141.

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2021 ha sido un año de grandes e importantes cambios para el MICI. A nuestro contexto general, marcado por un año más de la excepcionalidad del COVID y las consiguientes dificultades adicionales en la gestión de casos y el conjunto de actividades, se le sumó el relevo de parte de nuestro equipo. A partir de la evaluación de OVE, hemos puesto en marcha un plan de acción que nos permitirá avanzar en los próximos cinco años en el fortalecimiento del sistema de rendición de cuentas del Grupo BID y los resultados ambientales y sociales de los proyectos que financia. Respecto a los casos que hemos atendido, durante este año se normalizó el número de reclamos tras la caída registrada en el primer año de pandemia. En dos procesos de diálogo activos en Fase de Consulta se lograron acuerdos entre las comunidades afectadas, los responsables de los proyectos y el BID. Y en Fase de Verificación de la Observancia se completó una compleja investigación con aspectos innovadores para incidir más en la sustentabilidad de los proyectos del Grupo BID.
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