Academic literature on the topic 'Hémolysat'
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Journal articles on the topic "Hémolysat"
Chianéa, Denis, Fabienne Adam, Jean-Louis Capron, and Hubert Corbé. "Adaptation d'une méthode colorimétrique à la mesure automatisée de l'activité catalytique des cholinestérases dans un plasma et dans un hémolysat de sang total." Annales de Toxicologie Analytique 13, no. 1 (2001): 41–48. http://dx.doi.org/10.1051/ata/2002027.
Full textBauduer, F. "Hémolyses extracorpusculaires non immunologiques et hémolyses d'origine toxique." EMC - Hématologie 4, no. 4 (January 2009): 1–7. http://dx.doi.org/10.1016/s1155-1984(09)50260-9.
Full textOuragh, Lahoussine, and Mohammed Bengoumi. "Le polymorphisme des protéines sanguines chez le dromadaire (Camelus dromedarius) au Maroc." Revue d’élevage et de médecine vétérinaire des pays tropicaux 49, no. 4 (April 1, 1996): 347–48. http://dx.doi.org/10.19182/remvt.9509.
Full textReynier, V., C. Borne, C. Viliot, C. Vidy, B. Luche, D. Ferley, J. L. Lafond, and F. Carpentiera. "Des prélèvements hémolysés : que faire ?" Journal Européen des Urgences 21 (March 2008): A240. http://dx.doi.org/10.1016/j.jeur.2008.03.144.
Full textRieux, Claire, Edith De Meyer, Dora Bachir, and Karim Boudjedir. "Hémolyses drépanocytaires : où en sommes-nous ?" Transfusion Clinique et Biologique 23, no. 4 (November 2016): 271–72. http://dx.doi.org/10.1016/j.tracli.2016.08.147.
Full textBlanche, P., A. Rigolet, PP Massault, F. Dreyfus, and D. Sicard. "Hémolyse auto-immune et miliaire tuberculeuse." La Revue de Médecine Interne 19 (January 1998): 461. http://dx.doi.org/10.1016/s0248-8663(98)90186-2.
Full textMalezieux-Picard, A., V. Queyrel, P. Y. Jeandel, J. G. Fuzibet, B. Baldin, H. Hivernat, and R. Lazdunski. "Une hémolyse qui n’en finit pas !" La Revue de Médecine Interne 36 (December 2015): A136. http://dx.doi.org/10.1016/j.revmed.2015.10.081.
Full textLevoir, L., H. Petit, A. Desclaux, E. Trochut, C. Ragot, C. Constant, M. Jeanne, and A. Assal. "Drépanocytose et décès par hémolyse retardée." Transfusion Clinique et Biologique 20, no. 3 (June 2013): 354. http://dx.doi.org/10.1016/j.tracli.2013.03.225.
Full textAloni, N. M., M. Nsangu, T. Kunuanunua, T. B. Kadima, and T. F. Muanda. "Hémolyse intravasculaire après prise d’artéméther-luméfantrine." Bulletin de la Société de pathologie exotique 103, no. 5 (July 23, 2010): 296–98. http://dx.doi.org/10.1007/s13149-010-0076-6.
Full textDesnos-Malchiodi, P., C. Zenner-Zerrouqa, D. Bastit, and B. Dureuil. "Une hémolyse peut en cacher une autre." Annales Françaises d'Anesthésie et de Réanimation 16, no. 3 (January 1997): 297–300. http://dx.doi.org/10.1016/s0750-7658(97)86415-5.
Full textDissertations / Theses on the topic "Hémolysat"
Ibwala, Nsatam-Yamumi. "Escherichia coli :hémagglutination, hémolyse et adhésion." Doctoral thesis, Universite Libre de Bruxelles, 1989. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/213209.
Full textDelmotte, Nathanaël. "Développement de méthodes chromatographiques liquides multidimensionnelles couplées à la spectrométrie de masse, préparation et analyse d'échantillons biologiques complexes." Phd thesis, Université Louis Pasteur - Strasbourg I, 2007. http://tel.archives-ouvertes.fr/tel-00193714.
Full textSix matériaux à accès restreints ont été évalués en fonction de leur aptitude à exclure l'hémoglobine d'hémolysats sanguins. Des injections à différents pH ont montré que la rétention de l'hémoglobine est drastiquement restreinte à pH 10,7. En raison d'une bonne stabilité à pH basique, la colonne polymérique Biotrap 500 MS RAM a été retenue pour l'extraction d'antibiotiques d'hémolysats sanguins. Des extractions quantitatives d'analytes à faibles concentrations (200 pg/μL) ont été réalisées sans effet mémoire d'hémoglobine sur la colonne.
Un nouveau système 2D-HPLC-ESI-MS/MS pour l'analyse protéomique a été développé. Le système est composé d'une séparation par RP-HPLC à pH 10,0, suivie d'une séparation par IP-RP-HPLC à pH 2,1. Ce nouveau système a été comparé à un système conventionnel SCX x IP-RP-HPLC. L'orthogonalité des méthodes de séparation est plus élevée dans l'approche SCX x IP-RP-HPLC que dans le schéma RP x IP-RP-HPLC. Cependant, en raison d'une meilleure distribution des peptides et d'une meilleure efficacité de séparation, le système RP x IP-RP-HPLC permet d'identifier significativement plus de peptides. Les deux approches sont complémentaires et une combinaison des deux systèmes permet d'identifier plus de peptides que des analyses répétées par un système unique.
Didelon, Jacques. "Automatisation et validation d'un test de fragilité des hématies par hémolyse lente." Vandoeuvre-les-Nancy, INPL, 2001. http://www.theses.fr/2001INPL026N.
Full textMockesch, Berenike. "Caractérisation de la fonction micro et macrovasculaire chez les enfants drépanocytaires SS et SC : les effets de l'activité physique, du stress oxydant et les conséquences sur la sévérité clinique." Thesis, Antilles, 2017. http://www.theses.fr/2017ANTI0194/document.
Full textSickle cell disease is the most common genetic disease in the West Indies that affectshemoglobin. Because of considerable heterogeneity in clinical outcomes, its complex pathophysiology isstill not totally understood. For many decades it was widely assumed that blood disorders were the solecause of vaso-occlusion. More recently blood vessel dysfunction also seem to be involved. But it isunclear whether vascular function is affected similarly in children with sickle cell anaemia (SS) and childrenwith sickle haemoglobin C (SC) disease. This study with a cohort of Guadeloupean children demonstratedthat microvascular reactivity is impaired already in sickle cell SS and SC children compared to healthychildren. Divers biological and physiological analysis revealed that there are many interacting factors, all ofwhich may contribute to vasculopathy: (1) chronic endothelial stress due to elevated blood flow level, (2)reduced nitric oxide bioavailability due to chronic hemolysis and oxidative stress, (3) altered autonomicnervous system activity with increase risque in vasoconstrictor activity, (4) saturated antioxidant activityand (5) limited physical activity known to improve vascular function. In this children cohort, vascularimpairments were not associated to clinical severity. However vascular dysfunction seems to beexacerbated in SS. Mild haemolysis in SC, compared to SS, may limit oxidative and nitrosative stress andcould explain the better preserved microvascular function in this group. Learning more about the progressof vascular dysfunction in sickle cell disease seems to be essential. In advanced state it may possibleseriously worsen clinical severity
Aviat, Florence. "Étude d'une protéine de leptospires : Hemolysis Associated Protein 1 : Hap 1." Nantes, 2006. http://www.theses.fr/2006NANT2012.
Full textLeptospirosis is a zoonosis with a worldwide distribution concerning most Mammalians among which humans. Hap1 was purified from an antigenic zone with an apparent molecular mass of 32 kDa extracted from leptospires, bacteria responsible for leptospirosis. Immunization with Hap1 expressed by adenovirus or plasmid induced in gerbils a protection against leptospirosis. Then hap1 gene was expressed by E. Coli to product the recombinant protein rHap. This present work confirms these previous data: Hap1 is secreted during the multiplication of only pathogenic leptospires. Very immunogenic and protective in the natural conditions, this protein in the recombinant form doesn't permit to reproduce protective activity in the natural conditions. So, the purpose of this work was to purify one or many Hap1 naturally forms in order to determine the structural difference to understand this contradiction
Chwalek, Martin. "Hémisynthèse de saponosides à hédéragénine. Etude de l'influence de la chai͏̈ne osidique sur l'activité hémolytique." Reims, 2004. http://theses.univ-reims.fr/exl-doc/GED00000001.pdf.
Full textThis @PhD thesis presents the synthesis of nine disaccharides consisting of a-L-rhamnopyranose/a-L-arabinopyranose, ß-D-xylopyranose/a-L-arabinopyranose and ß-D-glucopyranose/a-L-arabinopyranose. The anomeric position of these disaccharides were then activated and these compounds were coupled to a hederagenin derivative, giving access, after total deprotection to a serie of 12 saponins. A second serie of hederagenin saponins (D-cellobiose, D-lactose, D-maltose, D-melibiose, D-gentiobiose or D-isomaltose osidic moiety) was also synthesized by the trichloroacetimidate glycosylation method. All of the resulting saponins were then tested for their hemolytic activity as either the free carboxylic acid or as the coresponding methyl ester in order to establish structure-activity relationships. The cytotoxic activity of the free carboxylic acid saponins derivative from a-hederin, was also measured in vitro on KB cells. Another part of this thesis deals with the synthesis of isomaltose derivatives. Isomaltose trichloroacetimidate was synthesized in five steps from D-amygdalin by acid catalyzed rearrangement of the interglycosidic bond
Narbey, David. "Epidémiologie des hémolyses post transfusionnelles retardées chez les patients drépanocytaires adultes : incidence, facteurs de risque et construction d’un score prédictif." Thesis, Paris Est, 2017. http://www.theses.fr/2017PESC0017.
Full textDelayed hemolytic transfusion reaction (DHTR) is a life-threatening complication of transfusion in sickle cell disease (SCD). The frequency of DHTR is underestimated and no predictive fac-tors for identifying patients likely to develop DHTR have yet been defined. We conducted a prospective single-center observational study over 30 months. We included 694 transfusion epi-sodes (TE) in 311 adult patients, divided into occasional TE (OTE: 360) and TE during a chronic program (CTE: 334). During follow-up, 15 cases of DHTR were recorded, exclusively after OTEs. DHTR cumulative incidence durin the 30 months was 4.2% per OTE (95% CI [2.6, 6.9]) or 66.8% per patient (95% CI [4.2, 11.3]). The incidence rate was 16.4 DHTR per 1000 OTE-Year (95% CI [10.1, 27.2]) or 27.1 DHTR per 1000 Person-Year (95% CI [16.7 ; 45.0]). We studied 11 additional DHTR cases to construct a score for predicting DHTR after OTE. Fifteen % of the 26 DHTR patients died. The variables retained in the multivariate model were history of DHTR, number of units previously transfused and immunization status before transfusion. The score adequacy was excellent (Hosmer-Lemeshow = 1.40, p = 0.71), very satisfactory dis-criminant capacity (area under ROC curve = 0.85, p <0.0001), negative-predictive value of 98.4% and a positive-predictive value of 50%. The internal validity of the score, realized by Bootstrap, shows a very good performance.We report, for the first time, the incidence of DHTR and we show that DHTR developed only in OTE. We also describe a simple score for predicting DHTR in patients undergoing occasional transfusion, to facilitate the management of blood transfusion in SCD patients
Manaargadoo-Catin, Magalie. "Etude des interactions entre les cellules sanguines et les tensioactifs." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS029/document.
Full textSurfactants such as saponins are employed, in the haematology field, as erythrolytic agent in order to count and to identify different leukocyte populations, which are typically thousand times less abundant than erythrocytes. These saponins are used for erythrocytes selective lysis in HORIBA Medical reagents. This thesis aimed to study the action of different surfactants on red blood cells in order to have a better understanding of surfactants/membrane interactions. The first project involved the observation and the characterization of saponins effects on erythrocytes by microscopic and spectrophotometric methods, in order to understand saponins selectivity regarding erythrocytes. We have thus demonstrated the participation of cholesterol, transporters such as band 3 and erythrocyte cytoskeleton during saponins lysis. In a second study, investigations have been performed in order to identify surfactants properties required to get a suitable erythrocyte lysing agent. We have chosen two surfactants families: non-ionic surfactants of the polyoxyethylene alkyl ethers series and anionic amino acid-based surfactants. These studies are focused on the relation between surfactants erythrolytic potency and some physico-chemical parameters such as the critical micellar concentration (CMC), the hydrophile-lipophile balance (HLB), the surfactants membrane / water partition coefficient (Kb) and the packing parameter (P). We have thus shown that the global consideration of all these physico-chemical parameters was necessary to predict surfactants interactions with the erythrocytes membrane. Finally, the lytic effects of surfactants on leukocytes were studied in flow cytometry to verify leukocytes integrity during different lysis. The results obtained in this thesis make possible the future development of hematological reagent for leukocytes differentiation
Bardakdjian, Josiane. "Contribution à l'étude des mécanismes moléculaires de l'instabilité de l'hémoglobine humaine." Paris 12, 1994. http://www.theses.fr/1994PA120057.
Full textHamdoune, Faouzia. "Synthèses et évaluations des propriétés de fluorolipopeptides non ioniques monodispersés." Nancy 1, 1990. http://www.theses.fr/1990NAN10497.
Full textBook chapters on the topic "Hémolysat"
Béné, Marie Christine, Patricia Martinez-Aguilar, Dominique Lasne, France Pirenne, Valérie Ugo, Anne-Marie Fischer, Nadine Ajzenberg, Claude Preudhomme, and Marc Maynadié. "Exploration des anomalies du métabolisme du fer et hémolyse." In Guide des Analyses en Hématologie, 59–68. Elsevier, 2018. http://dx.doi.org/10.1016/b978-2-294-75359-6.00004-4.
Full textde Montalembert, Mariane, Slimane Allali, and Thierry Peyrard. "Allo-immunisation anti-érythrocytaire et hémolyse post-transfusionnelle retardée." In La Drépanocytose de L'enfant et L'adolescent, 187–96. Elsevier, 2020. http://dx.doi.org/10.1016/b978-2-294-76049-5.00025-4.
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