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Journal articles on the topic 'Herb Dad'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Liu, Yuan, Gongjun Yang, and Fang Feng. "Integrated chemical profiling of Zhi-Zi-Hou-Po decoction by liquid chromatography-diode array detector-time of flight mass analyzer and liquid chromatography-triple stage quadrupole mass analyzer combined with chemometrics." Analytical Methods 8, no. 23 (2016): 4689–710. http://dx.doi.org/10.1039/c6ay01233g.

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Basaraba, Roksolana, Alona Savych, Svitlana Marchyshyn, Nataliіa Muzyka, and Pavlina Ilashchuk. "HPLC-DAD assay of phenols profile in Antennaria dioica (L.) Gaertn." Pharmacia 69, no. 2 (2022): 393–99. http://dx.doi.org/10.3897/pharmacia.69.e81654.

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Antennaria dioica (Asteraceae family) – is a perennial herb, commonly found in dry grasslands and sandy or stony places from Eurasian areas. It is known in traditional medicine as antioxidant, diuretic, choleretic and anti-inflammatory remedy. This species should be reconsidered as possible sources of phenols, mainly flavonoids and hydroxycinnamic acids. Thus, the aim of this study was to validate a chromatographic method for detection of phenols and their identification in A. dioica herb. HPLC-DAD method was evaluated in terms of linearity, precision, repeatability, accuracy, LOD and LOQ. The

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Basaraba, Roksolana, Alona Savych, Svitlana Marchyshyn, Nataliіa Muzyka, and Pavlina Ilashchuk. "HPLC-DAD assay of phenols profile in Antennaria dioica (L.) Gaertn." Pharmacia 69, no. (2) (2022): 393–99. https://doi.org/10.3897/pharmacia.69.e81654.

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Antennaria dioica (Asteraceae family) – is a perennial herb, commonly found in dry grasslands and sandy or stony places from Eurasian areas. It is known in traditional medicine as antioxidant, diuretic, choleretic and anti-inflammatory remedy. This species should be reconsidered as possible sources of phenols, mainly flavonoids and hydroxycinnamic acids. Thus, the aim of this study was to validate a chromatographic method for detection of phenols and their identification in A. dioica herb. HPLC-DAD method was evaluated in terms of linearity, precision, repeatability, accuracy, LOD and LOQ. The

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Wang, Tianyi, Sen Guo, Shanshan Zhang, Wenping Yue, Chi-Tang Ho, and Naisheng Bai. "Identification and quantification of seven sesquiterpene lactones in Inula britannica by HPLC-DAD-MS." Analytical Methods 11, no. 13 (2019): 1822–33. http://dx.doi.org/10.1039/c9ay00118b.

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Węglarz, Zenon, Olga Kosakowska, Ewelina Pióro-Jabrucka, et al. "Antioxidant and Antibacterial Activity of Helichrysum italicum (Roth) G. Don. from Central Europe." Pharmaceuticals 15, no. 6 (2022): 735. http://dx.doi.org/10.3390/ph15060735.

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Helichrysum italicum (Roth) G. Don. is one of the most important cosmetic and medicinal plants originating from the Mediterranean region of Europe. The aim of this study was to assess the chemical profile as well as antioxidant and antibacterial potential of the species cultivated in the temperate climate of Central Europe. The analyses were carried out using herbs and inflorescences. The content of essential oil ranged from 0.25 g × 100 g−1 in the herb to 0.31 g × 100 g−1 in the inflorescences. Neryl acetate, accompanied by α-pinene in the herb (10.42%), and nerol in inflorescences (15.73%) w

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Demydiak, Diana, Liudmyla Slobodianiuk, Oleg Gerush, et al. "HPLC-DAD analysis of flavonoids and hydroxycinnamic acids in Aster novi-belgii L." Pharmacia 70, no. 3 (2023): 745–50. http://dx.doi.org/10.3897/pharmacia.70.e94344.

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Aster novi-belgii is a perennial ornamental herb native to eastern Canada and the United States of America, cultivated in Ukraine. This species should be considered a possible source of phenolic compounds, principally hydroxycinnamic acids and flavonoids. Therefore, in this study, the aim was to determine these compounds in Aster novi-belgii by HPLC-DAD analysis, and validation of this chromatographic method and lay a scientific and technical basis for the utilization and development of the plant resources of the cultivated plants of the genus Aster. The HPLC-DAD method determined the flavonoi

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Demydiak, Diana, Liudmyla Slobodianiuk, Oleg Gerush, et al. "HPLC-DAD analysis of flavonoids and hydroxycinnamic acids in Aster novi-belgii L." Pharmacia 70, no. (3) (2023): 745–50. https://doi.org/10.3897/pharmacia.70.e94344.

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Aster novi-belgii is a perennial ornamental herb native to eastern Canada and the United States of America, cultivated in Ukraine. This species should be considered a possible source of phenolic compounds, principally hydroxycinnamic acids and flavonoids. Therefore, in this study, the aim was to determine these compounds in Aster novi-belgii by HPLC-DAD analysis, and validation of this chromatographic method and lay a scientific and technical basis for the utilization and development of the plant resources of the cultivated plants of the genus Aster. The HPLC-DAD method determined the flavonoi

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Hroboňová, Katarína, Jana Sádecká, and Jozef Čižmárik. "HPLC separation and determination of dicoumarol and other simple coumarins in sweet clover." Nova Biotechnologica et Chimica 17, no. 1 (2018): 95–102. http://dx.doi.org/10.2478/nbec-2018-0010.

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AbstractDicoumarol is a mycotoxin, that acts as a blood anticoagulant, is formed during the microbial action of molds and fungi in spoiled hay or silage containing high-coumarin plant. A HPLC-DAD method for determination of coumarins, including dicoumarol, coumarin, and 4-hydroxycoumarin was developed. Methanol and acetic acid were used as mobile phase with gradient elution. The simultaneous separation was performed using C18 type of stationary phase. The recoveries were 88.6 – 92.6 %, 91.8 – 95.0 %, and 89.7 – 94.1 % (evaluated for three concentration levels) for dicoumarol, coumarin, and 4-h

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Nikolić, Valentina, Jelena Zvezdanović, and Sandra Konstantinović. "UHPLC-DAD-ESI-MS analysis of the 'Centaurium erythraea' infusion." Advanced Technologies 11, no. 1 (2022): 13–21. http://dx.doi.org/10.5937/savteh2201013n.

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Chemical composition was estimated in a tea sample, i.e. infusion prepared from dried aerial herb of Centaurium erythraea. The plant was harvested in the Stara Planina mountain (Serbia, 42°43'00"N; 24°55'04"E) during the flowering stage. The flowering tops of the plant were dried to the moisture content of 7.89 (w/w) in a dark place. Qualitative analysis was done by Ultrahigh performance liquid chromatography-diode array detector-electrospray mass spectrometry (UH-PLC-DAD-ESI-MS/MS) method. The aim of this study was to detect and identify phytochemicals in the infusion prepared in a traditiona

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Shishmareva, Tat'yana Mikhaylovna, Vyacheslav Mikhaylovich Shishmarev, and Daniil Nikolayevich Olennikov. "PHENOLIC COMPOUNDS SANGUISORBA OFFICINALIS (ROSACEAE) GROWING IN EASTERN SIBERIA." chemistry of plant raw material, no. 1 (March 16, 2021): 139–50. http://dx.doi.org/10.14258/jcprm.2021018281.

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The present work aimed at the study of the phenolic compounds of Sanguisorba officinalis L. (Rosaceae) growing in Eastern Siberia. The quantitative content of flavonoids in S. officinalis extracts determined by the spectrophotometric method was 0.68–5.41% in herb and 0.20–0.51% in rhizomes and roots. Catechin content was 0.05–0.75% in herb and 0.08–9.86% in rhizomes and roots, and procyanidin content found with spectrophotometric Porter method was 0.02–1.45% in herb and 0.08–1.05% in rhizomes and roots. Regression analysis of the relationships “latitude / longitude / altitude – phenolic compou

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Ding, Yubin, Ksenia Morozova, Matteo Scampicchio, Angelo Morini, and Massimiliano Ferrari. "A novel cascade approach to extract bioactive compounds from officinal herbs." Italian Journal of Food Science 34, no. 2 (2022): 1–8. http://dx.doi.org/10.15586/ijfs.v34i2.2137.

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This research aims to compare a novel cascade extraction method with a conventional solid–liquid extraction method, both assisted by ultrasounds. The cascade extraction method consists of a sequential series of extractions performed with the same hydroalcoholic solvent, which is reused from one herb to the other. In practice, a hydroalcoholic solution is firstly used to extract one botanical herb. The resulting extract is then reused for the extraction of a second herb. The process is repeated as many times as the number of herbs composing the final formulation. The main advantage of this appr

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Michalak, Monika, Martyna Zagórska-Dziok, Marta Klimek-Szczykutowicz, and Agnieszka Szopa. "Phenolic Profile and Comparison of the Antioxidant, Anti-Ageing, Anti-Inflammatory, and Protective Activities of Borago officinalis Extracts on Skin Cells." Molecules 28, no. 2 (2023): 868. http://dx.doi.org/10.3390/molecules28020868.

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In this study, methanol and water–methanol extracts of borage (Borago officinalis) herb dried using various methods were analysed for their phenolic profile and biological activity. Twelve compounds, including flavonoids (astragalin, kaempferol 4-glucoside, rutoside, and vitexin) and phenolic acids (caffeic, chlorogenic, 3,4-dihydroxyphenylacetic, ferulic, p-hydroxybenzoic, protocatechuic, rosmarinic, and syringic), were determined qualitatively and quantitatively in B. officinalis extracts by the HPLC-DAD method. The highest total flavonoid content was confirmed for the methanol extract from

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Savych, Alona, Svitlana Marchyshyn, Olha Polonets, Olga Mala, Iryna Shcherba, and Liubov Morozova. "HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures." Pharmacia 69, no. 3 (2022): 873–81. http://dx.doi.org/10.3897/pharmacia.69.e86486.

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Medicinal plants and their combinations can influence various links of the pathogenetic mechanism of diabetes mellitus type 2 and its complications, due to the wide range of biologically active substance that they accumulate. Flavonoids deserve particular attention through their antioxidant properties. Three samples of herbal mixtures (sample 1 – Inula helenium rhizome with roots, Helichrysum arenarium flowers, Zea mays columns with stigmas, Origanum vulgare herb, Rosa majalis fruits, Taraxacum officinale roots; sample 2 – Cichorium intybus roots, Elymus repens rhizome, Helichrysum arenarium f

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Olennikov, Gadimli, Isaev, et al. "Caucasian Gentiana Species: Untargeted LC-MS Metabolic Profiling, Antioxidant and Digestive Enzyme Inhibiting Activity of Six Plants." Metabolites 9, no. 11 (2019): 271. http://dx.doi.org/10.3390/metabo9110271.

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The members of Gentiana genus are widely distributed in the Caucasus region where they are used as phytoremedies, but they still have not been studied for their chemical composition and bioactivity. High-performance liquid chromatography with diode array and electrospray triple quadrupole mass detection (HPLC-DAD-ESI-QQQ-MS) was used to investigate metabolites of herb and roots of six gentians (Gentiana asclepiadea, G. cruciata, G. gelida, G. paradoxa, G. pneumonanthe, G. septemfida) grown in the Caucasus. In total, 137 compounds were found including three carbohydrates, 71 iridoid glycosides

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15

Savych, Alona, Svitlana Marchyshyn, Olha Polonets, Olga Mala, Iryna Shcherba, and Liubov Morozova. "HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures." Pharmacia 69, no. (3) (2022): 873–81. https://doi.org/10.3897/pharmacia.69.e86486.

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Medicinal plants and their combinations can influence various links of the pathogenetic mechanism of diabetes mellitus type 2 and its complications, due to the wide range of biologically active substance that they accumulate. Flavonoids deserve particular attention through their antioxidant properties. Three samples of herbal mixtures (sample 1 – Inula helenium rhizome with roots, Helichrysum arenarium flowers, Zea mays columns with stigmas, Origanum vulgare herb, Rosa majalis fruits, Taraxacum officinale roots; sample 2 – Cichorium intybus roots, Elymus repens rhizome, Helichrysum arenarium f

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16

Ilina, Tetiana, Natalia Kashpur, Sebastian Granica, et al. "Phytochemical Profiles and In Vitro Immunomodulatory Activity of Ethanolic Extracts from Galium aparine L." Plants 8, no. 12 (2019): 541. http://dx.doi.org/10.3390/plants8120541.

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Galium aparine L., family Rubiaceae, is a widely spread species in the Galium genus. The herb of G. aparine is part of folk remedies and dietary supplements. In this study, we analyzed the chemical composition and immunomodulatory activities of G. aparine herb ethanolic extracts obtained from the plant material by maceration with 20%, 60% or 96% ethanol. The contents of hydroxycinnamic acid derivatives, flavonoids and polyphenols were determined spectrophotometrically, with extractives and polysaccharides quantified gravimetrically. The qualitative composition was studied using UHPLC-DAD-MS/MS

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17

Yang, Yanfang, Guijun Zhang, Qiyu Sun, et al. "Simultaneous Determination of 8 Compounds in Gancao-Ganjiang-Tang by HPLC-DAD and Analysis of the Relations between Compatibility, Dosage, and Contents of Medicines." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/4703632.

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Gancao-Ganjiang-Tang (GGT) is a traditional Chinese medicine (TCM) prescription and is a representative prescription for recuperating depleted Yang in Treatise on Febrile Diseases. The TCM theory believes that the efficacy of medicinal herbs is decided by the multicompounds which consist of different kinds of chemical constituents with bioactivities, but not by a monomeric constituent. From ancient times until today, GGT have 5 different kinds of compatibilities that can be verified. In this study, a HPLC-DAD method was established for the simultaneous determination of 8 compounds including 6-

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18

Olennikov, Daniil N., Innokentii A. Fedorov, Nina I. Kashchenko, Nadezhda K. Chirikova, and Cecile Vennos. "Khellactone Derivatives and Other Phenolics of Phlojodicarpus sibiricus (Apiaceae): HPLC-DAD-ESI-QQQ-MS/MS and HPLC-UV Profile, and Antiobesity Potential of Dihydrosamidin." Molecules 24, no. 12 (2019): 2286. http://dx.doi.org/10.3390/molecules24122286.

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With obesity, the consumption of phenolic-enriched food additives as a part of traditional nutrition avoids the negative implications of eating high-calorie products. This study investigated the new herbal food additive, Phlojodicarpus sibiricus roots and herb, ubiquitously used in Siberia as a spice. Chromatographic techniques such as HPLC-DAD-ESI-QQQ-MS/MS and microcolumn HPLC-UV were the basic instruments for component profiling and quantification, and antiobesity potential was investigated using a differentiated 3T3-L1 adipocytes assay. We found that the roots and herb of P. sibiricus were

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19

Petruczynik, Anna, Tomasz Plech, Tomasz Tuzimski, et al. "Determination of Selected Isoquinoline Alkaloids from Mahonia aquifolia; Meconopsis cambrica; Corydalis lutea; Dicentra spectabilis; Fumaria officinalis; Macleaya cordata Extracts by HPLC-DAD and Comparison of Their Cytotoxic Activity." Toxins 11, no. 10 (2019): 575. http://dx.doi.org/10.3390/toxins11100575.

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Alkaloids have protective functions for plants and can play an important role in living organisms. Alkaloids may have a wide range of pharmacological activities. Many of them have cytotoxic activity. Nowadays, cancer has become a serious public health problem. Searching for effective drugs with anticancer activity is one of the most significant challenges of modern scientific research. The aim of this study was the investigation of cytotoxic activity of extracts obtained from Corydalis lutea root and herb, Dicentra spectabilis root and herb, Fumaria officinalis, Macleaya cordata leaves and her

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20

Sieniawska, E., T. Baj, J. Dudka, T. Mroczek, and K. Głowniak. "TLC-DPPH•activity-guided separation and LC-DAD-MS identification of antioxidant compounds fromMutellina purpureaL. herb." Acta Chromatographica 28, no. 1 (2016): 51–58. http://dx.doi.org/10.1556/achrom.28.2016.1.5.

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21

Napierała, Marta, Joanna Nawrot, Justyna Gornowicz-Porowska, et al. "Separation and HPLC Characterization of Active Natural Steroids in a Standardized Extract from the Serratula coronata Herb with Antiseborrheic Dermatitis Activity." International Journal of Environmental Research and Public Health 17, no. 18 (2020): 6453. http://dx.doi.org/10.3390/ijerph17186453.

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Phytoecdysteroids are natural compounds with therapeutic benefits in both humans and animals. The effectiveness of natural products with health potential is based on the activities and potencies of their active ingredients. In this study, dominant ecdysteroids—ajugasterone C, 20-hydroxyecdysone and polypodine B—from the Serratula coronata (S. coronata) herb were separated by column chromatography, identified by spectroscopic data and quantified by high-performance liquid chromatography with a diode array detector (HPLC-DAD). The obtained concentration of ecdysteroids (approximately 23%) in the

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Laanet, Pille-Riin, Piret Saar-Reismaa, Piia Jõul, Olga Bragina, and Merike Vaher. "Phytochemical Screening and Antioxidant Activity of Selected Estonian Galium Species." Molecules 28, no. 6 (2023): 2867. http://dx.doi.org/10.3390/molecules28062867.

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The aim of the present study was to examine three different Galium species from the native population of Estonia, Galium verum, Galium aparine, and Galium mollugo, to characterise their non-volatile and volatile phytochemical composition and antioxidant activity. The main groups of bioactive compounds in the plants were quantified by colorimetric tests, showing high concentrations of polyphenols (up to 27.2 ± 1.5 mg GAE/g), flavonoids (up to 7.3 ± 0.5 mg QE/g) and iridoids (up to 40.8 ± 2.9 mg AE/g). The species were compared using HPLC-DAD-MS/MS, revealing some key differences in the phytoche

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Willer, Johanna, Karin Jöhrer, Richard Greil, Christian Zidorn, and Serhat Çiçek. "Cytotoxic Properties of Damiana (Turnera diffusa) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay." Molecules 24, no. 5 (2019): 855. http://dx.doi.org/10.3390/molecules24050855.

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In our continuing search for new cytotoxic agents, we assayed extracts, fractions, and pure compounds from damiana (Turnera diffusa) against multiple myeloma (NCI-H929, U266, and MM1S) cell lines. After a first liquid-liquid solvent extraction, the ethyl acetate layer of an acetone (70%) crude extract was identified as the most active fraction. Further separation of the active fraction led to the isolation of naringenin (1), three apigenin coumaroyl glucosides 2–4, and five flavone aglycones 5–9. Naringenin (1) and apigenin 7-O-(4″-O-p-E-coumaroyl)-glucoside (4) showed significant cytotoxic ef

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Husain, Tasneem. "Scientific Validation of Bioactive Compound Psoralen from Psoralea corylifolia Seeds Extract Using HPLC-DAD-TOFMS Analysis." Bioscience Biotechnology Research Communications 18, no. 1 (2025): 62–66. https://doi.org/10.21786/bbrc/18.1.9.

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Psoralea corylifolia Linn. is an herb from the Fabaceae family, used in both Indian and Chinese traditional medicine. Commonly known as Babchi, it is a traditional medicinal plant widely utilized in Ayurveda and Unani medicine. Psoralen, a major bioactive furocoumarin found in its seeds, exhibits various pharmacological activities. In this study, High-Performance Liquid Chromatography coupled with Diode-Array Detection and Time-of-Flight Mass Spectrometry (HPLC-DAD-TOFMS) was employed to validate and quantify psoralen in P. corylifolia seed extracts. The characteristic retention time (Rt) of t

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25

Huang, Ho-Shin, Hsu-Sheng Yu, Chia-Hung Yen, and Ean-Tun Liaw. "HPLC-DAD-ESI-MS Analysis for Simultaneous Quantitation of Phenolics in Taiwan Elderberry and Its Anti-Glycation Activity." Molecules 24, no. 21 (2019): 3861. http://dx.doi.org/10.3390/molecules24213861.

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Sambucus formosana is most commonly used as a traditional herb medicine in Taiwan. In this study, high performance liquid chromatography equipped with photodiode array detection-mass (HPLC–DAD-ESI-MS) method was developed for the identification and quantification of bioactive phenolics. The developed method was also validated for accuracy, precision, limit of detection, and quantification. In this method, chlorogenic acid, rutin, isoquercetrin, nictoflorin, astragalin, and quercetin were quantified in linearity range of 10–100 (μg/mL) with a correlation coefficient of greater than 0.996. High

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26

Toth, Anita, Gergo Toth, and Agnes Kery. "Polyphenol Composition and Antioxidant Capacity of Three Lysimachia Species." Natural Product Communications 9, no. 10 (2014): 1934578X1400901. http://dx.doi.org/10.1177/1934578x1400901017.

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The polyphenol composition and antioxidant properties of three Lysimachia species ( L. nummularia L., L. vulgaris L. and L. punctata L.) and their column chromatographic fractions were investigated. The antioxidant activity of herb extracts and 54 different column chromatographic fractions was evaluated using in vitro DPPH• and ABTS•+ decolorization tests. The total polyphenol content was determined by spectrophotometric methods. The phenolic compounds of extracts of different Lysimachia species and their bioactive fractions were characterized by online chromatographic methods. For identificat

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27

Jankovic, Mina, Sanja Berezni, and Dejan Orcic. "Lignan profile in fruits of wild chervil (Anthriscus sylvestris (L.) Hoffm.)." Facta universitatis - series: Physics, Chemistry and Technology 21, no. 1 (2023): 27–37. http://dx.doi.org/10.2298/fupct2301027j.

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Wild chervil (Anthriscus sylvestris (L.) Hoffm.), known also as cow parsley, is a widely distributed herbaceous plant from Apiaceae family. Traditional use as analgesic, antipyretic, antitussive, tonic, diuretic etc. is associated with the proven presence of various types of biomolecules, primarily lignans that possess cytotoxic, antiproliferative, insecticidal, antiviral and other activities. The majority of lignans reported in A. sylvestris belong to two classes - aryltetralins and dibenzylbutyrolactones. The data of lignan profile of A. sylvestris are mostly limited to root and herb composi

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Sheychenko, Olga Petrovna, Vladimir Ivanovich Sheychenko, Sergey Vladimirovich Goryainov, et al. "CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF THE DRY EXTRACT "ROSMATIN" FROM THE HERB OF DRACOCEPHALUM MOLDAVICA L." chemistry of plant raw material, no. 3 (September 27, 2021): 253–64. http://dx.doi.org/10.14258/jcprm.2021039161.

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Moldavian Dragonhead (Dracocephalum moldavica L.) is an annual herb. In FGBNU VILAR a new variety of Moldavian Dragonhead «Nezhnost» has been developed. From the herb of the Moldavian Dragonhead, a technology has been developed for obtaining a dry purified extract, which has received the code name «Rozmatin». By NMR (1H and 13C) and HPLC-DAD-MS using standard samples and literature data, the qualitative and quantitative composition of the dry extract "Rozmatin" from the herb of the Dracocephalum moldavica was established. Rosmarinic, caffeic and ferulic acids, rosmarinic acid 3-O-glucosi

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Misiurek, Justyna, Tomasz Plech, Barbara Kaproń, et al. "Determination of Some Isoquinoline Alkaloids in Extracts Obtained from Selected Plants of the Ranunculaceae, Papaveraceae and Fumarioideae Families by Liquid Chromatography and In Vitro and In Vivo Investigations of Their Cytotoxic Activity." Molecules 28, no. 8 (2023): 3503. http://dx.doi.org/10.3390/molecules28083503.

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Alkaloids are heterocyclic bases with widespread occurrence in nature. Plants are rich and easily accessible sources of them. Most isoquinoline alkaloids have cytotoxic activity for different types of cancer, including malignant melanoma, the most aggressive type of skin cancer. The morbidity of melanoma has increased worldwide every year. For that reason, developing new candidates for anti–melanoma drugs is highly needed. The aim of this study was to investigate the alkaloid compositions of plant extracts obtained from Macleaya cordata root, stem and leaves, Pseudofumaria lutea root and herb,

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30

El-Nakhel, Christophe, Antonio Pannico, Giulia Graziani, et al. "Mineral and Antioxidant Attributes of Petroselinum crispum at Different Stages of Ontogeny: Microgreens vs. Baby Greens." Agronomy 11, no. 5 (2021): 857. http://dx.doi.org/10.3390/agronomy11050857.

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Parsley is an aromatic herb native to the Mediterranean region and treasured for its phytochemical profile and bioactive properties. Developmental stage at harvest is a factor that modulates the nutritional quality of vegetables, including young greens. Accordingly, an experiment under strictly controlled conditions was carried out to compare the mineral macronutrient and phytochemical composition as well as the antioxidant activity of plain-leaf parsley (Petroselinum crispum cv. Comune 2) at two different harvest maturity stages, microgreens and baby greens. Macronutrients, carotenoids (lutei

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Tříska, Jan, Naděžda Vrchotová, Jan Strohalm, et al. "Effect of Ascorbic Acid Addition on the Phenolic Compounds Content in Homogenates from Aerial Parts of Spearmint, Fennel, and Thyme." Foods 14, no. 13 (2025): 2165. https://doi.org/10.3390/foods14132165.

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The paper deals with the investigation of the ascorbic acid influence on the analytical results of polyphenol content in the samples of the spearmint, fennel, and thyme homogenates. The homogenates without and with addition of ascorbic acid and water were prepared and stabilized by high-pressure treatment. Their analysis was accomplished by high-performance liquid chromatography (HPLC) with DAD detection and by combination of liquid chromatography with mass spectrometry (LC-MS). Volatile terpenes were analyzed in all homogenates by combination of gas chromatography with mass spectrometry techn

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32

Szymborska-Sandhu, Izabela, Jarosław L. Przybył, Ewelina Pióro-Jabrucka, Agata Jędrzejuk, Zenon Węglarz, and Katarzyna Bączek. "Effect of Shading on Development, Yield and Quality of Bastard Balm Herb (Melittis melissophyllum L.)." Molecules 25, no. 9 (2020): 2142. http://dx.doi.org/10.3390/molecules25092142.

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The aim of the study was to assess the effects of Melittis melissophyllum shading on its development and accumulation of phenolics. Their content (verbascoside, apiin, luteolin-7-O-glucoside, coumarin, 3,4-dihydroxycoumarin, o-coumaric acid 2-O-glucoside as well as o-coumaric, p-coumaric, chlorogenic, caffeic, ferulic and cichoric acid) was determined in the herb using HPLC-DAD. The results showed that the content of abovementioned flavonoids and phenolic acids was highest in plants grown under full sunlight. On the other hand, a higher content of coumarin was observed in shaded plants, especi

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Mitreski, Ilija, Jasmina Petreska Stanoeva, Marina Stefova, Gjoshe Stefkov, and Svetlana Kulevanova. "Polyphenols in Representative Teucrium Species in the Flora of R. Macedonia: LC/DAD/ESI-MSn Profile and Content." Natural Product Communications 9, no. 2 (2014): 1934578X1400900. http://dx.doi.org/10.1177/1934578x1400900211.

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In the present work, the polyphenolic profile and content of four Teucrium species ( T. chamaedrys L., T. montanum L., T. polium L., T. scordium L.) from the Macedonian flora were examined. A LC/DAD/ESI-MS n chromatographic method was optimized and 31 phenolic compounds were identified, quantified and classified into four groups: hydroxycinnamic acid derivatives (2), phenylethanoid glycosides (12), flavonoid glycosides (11) and flavonoid aglycones (6). The total phenolic content (mg/g dry herb) ranged from 28.2 ( T. montanum), 30.9 ( T. scordium), 35.1 ( T. polium) to 52.1 ( T. chamaedrys). Ph

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Czerwińska, Monika E., Eleonora Kalinowska, Dominik Popowski, and Agnieszka Bazylko. "Lamalbid, Chlorogenic Acid, and Verbascoside as Tools for Standardization of Lamium album Flowers—Development and Validation of HPLC–DAD Method." Molecules 25, no. 7 (2020): 1721. http://dx.doi.org/10.3390/molecules25071721.

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Preparations from the flowers or herb of the white dead nettle (Lamium album L.) are recommended for the treatment of upper respiratory tract disorders or as a topical medication for mild inflammation of the throat, mouth, and skin. Taking into consideration the significance of L. album in traditional medicines across Europe, as well as the lack of studies describing the quantities of their most abundant constituents, we aimed to design a high-performance liquid chromatography coupled with diode-array detection (HPLC–DAD) method for potential standardization procedures of extracts from flowers

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Mudawi, Mahmoud M. E., Mohammed F. Abd El-wahab, Abdelhadi Y. A. Yassin, Rami S. Habeballa, and Mohammed M. Alshehri. "Evaluation of Anticonvulsant Activity and HPLC–DAD Profiling of Achillea fragrantissima (Gaisoom) Extracts Growing in Saudi Arabia." Asian Journal of Pharmaceutical Research and Health Care 9, no. 3 (2017): 92. http://dx.doi.org/10.18311/ajprhc/2017/15798.

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In Kingdom of Saudi Arabia <em>Achillea fragrantissima</em> is a fragrant, perennial herb has long been used medicinally for its analgesic properties among Rafha residents. The present study concluded that <em>Achillea fragrantissima</em> (Gaisoom) possess a potent anticonvulsant activity against PTZ induced seizures which may be due to GABAA agonistic activity and /or antioxidant activity. The results of the current study showed that the ethanolic extract of Gaisoom at doses of 300 mg/kg and 600 mg/kg has anticonvulsant activity in pentylenetetrazole and maximum electr

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Li, Chengyong, Hong Yang, Chenxi Zhao, Yizeng Liang, Xiaomei Wang, and Linqi Liu. "Simultaneous Determination of Saponins and Anthraquinones in the Herb-Pair of Ginseng and Ploygonum Multiforum by HPLC-DAD." Advanced Science Letters 10, no. 1 (2012): 196–201. http://dx.doi.org/10.1166/asl.2012.3710.

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Gao, Ruixi, Bingchen Han, Yanfeng Zeng, et al. "HPLC–DAD Analysis, SFE-CO2 Extraction, and Antibacterial Activity on Bioactive Compounds from Mosla chinensis Maxim." Molecules 28, no. 23 (2023): 7724. http://dx.doi.org/10.3390/molecules28237724.

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Mosla chinensis Maxim is an annual herb with many potential purposes in agricultural, industrial, and pharmaceutical fields. At present, the extract of the whole plant from M. chinensis has been proven to demonstrate antifungal, antioxidant, and anti-inflammatory activities. Previous studies focused on the enzyme pretreatment in hydrodistillation from M. chinensis. However, organic solvent or supercritical fluid carbon dioxide extraction (SFE-CO2) methods, which are commonly utilized in industry, have seldom been studied and cannot provide multiple evaluations of yield. In this work, we analys

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Tian, Fang, Qun-Jia Ruan, Ying Zhang, et al. "Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process." Journal of Analytical Methods in Chemistry 2020 (April 23, 2020): 1–8. http://dx.doi.org/10.1155/2020/8950324.

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We aimed to establish a quantitative analysis method of six constituents (5-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) in Artemisia capillaris (Yinchen) and its decoction by using HPLC coupled with DAD. Besides, the transformation paths of the six constituents were analyzed in decoction preparation processing. The analytical method was fully validated in terms of linearity, sensitivity, precision, repeatability, and recovery and applied to assess the transformation trend and quantitativ

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Balanescu, Fanica, Maria Daniela Ionica Mihaila, Geta Cârâc, et al. "Flavonoid Profiles of Two New Approved Romanian Ocimum Hybrids." Molecules 25, no. 19 (2020): 4573. http://dx.doi.org/10.3390/molecules25194573.

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Basil (Ocimum spp.) is a traditional herbal medicine abundant in antioxidants such as phenolic compounds. As part of a diet, this herb is proved to have some roles in decreasing the risk of cancer, and in the treatment of inflammation and neurodegenerative diseases. This study aims to explore the total phenolic and flavonoid content of two new basil hybrids growing in Romania, namely “Aromat de Buzau” (AB) and “Macedon” (MB). The antioxidant capacity of those two species was also analyzed by DPPH and cyclic voltammetry. Six different flavonoids, such as catechin (+), rutin, hyperoside, naringi

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Huang, Qi, Chunsong Cheng, Lili Li, Daiyin Peng, and Cun Zhang. "New Monitoring Strategy for the Quality Control in the Processing Practice of Scutellariae Radix." Current Pharmaceutical Analysis 16, no. 3 (2020): 303–11. http://dx.doi.org/10.2174/1573412915666190314121434.

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Background: Scutellariae Radix (Huangqin) is commonly processed into 3 products for different clinical applications. However, a simple analytical method for quality control has rarely been reported to quickly estimate the degree of processing Huangqin or distinguish differently processed products or unqualified Huangqin products. Objective: To study a new strategy for quality control in the processing practice of Huangqin. Methods: Seven kinds of flavonoids that mainly exist in Huangqin were determined by HPLC-DAD. Chromatographic fingerprints were established to study the variation and discip

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Tang, Dan, Hui-Jun Li, Jun Chen, Chao-Wei Guo, and Ping Li. "Rapid and simple method for screening of natural antioxidants from Chinese herb Flos Lonicerae Japonicae by DPPH-HPLC-DAD-TOF/MS." Journal of Separation Science 31, no. 20 (2008): 3519–26. http://dx.doi.org/10.1002/jssc.200800173.

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Salahshour, Babak, Sajjad Sadeghi, Hajar Nazari, and Kambiz Soltaninejad. "Determining Undeclared Synthetic Pharmaceuticals as Adulterants in Weight Loss Herbal Medicines." International Journal of Medical Toxicology and Forensic Medicine 10, no. 1 (2020): 26253. http://dx.doi.org/10.32598/ijmtfm.v10i1.26253.

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Background: The popularity and use of herbal medicines and supplements are growing worldwide. Herbal anti-obesity products have been considered as suitable alternatives to synthetic pharmaceuticals as they are introduced as harmless natural products. However, some manufacturers often add undeclared synthetic pharmaceuticals to the anti-obesity herbal medicine products to improve their efficacy and potency. The present study aimed to analyze herbal weight loss products collected from the drug market in Bojnurd City, Iran.Methods: Ninety-six herbal drug samples, as weight loss products, were obt

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Dreger, Mariola, Agnieszka Gryszczyńska, Milena Szalata, and Karolina Wielgus. "Content of sterols in in vitro propagated Chamerion angustifolium (L.) Holub plants." Herba Polonica 68, no. 3 (2022): 1–7. http://dx.doi.org/10.2478/hepo-2022-0016.

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Summary Introduction: Chamerion angustifolium (L.) Holub (syn. Epilobium angustifolium L.) plants have been used in the treatment and alleviating symptoms of mild Benign Prostatic Hyperplasia (BPH). Plants are abundant in polyphenols, particularly ellagitannins, phenolic acids and flavonoids. Apart from polyphenols, herb of this species also contains steroids, triterpenes and fatty acids. Objective: The aim of this study was to determine the content of campesterol, β-sitosterol and stigmasterol in C. angustifolium genotypes cultivated in vitro. Methods: Plants grown in vitro and the plants har

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ZHANG, Feng, Qi YANG, Lian-Na SUN, Shou-Hong GAO, Xia TAO, and Wan-Sheng CHEN. "Fingerprint analysis of Zhimu-Huangbai herb pair and simultaneous determination of its alkaloids, xanthone glycosides and steroidal saponins by HPLC-DAD-ELSD." Chinese Journal of Natural Medicines 12, no. 7 (2014): 525–34. http://dx.doi.org/10.1016/s1875-5364(14)60082-0.

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Nurfaradilla, Shinta Ayu, Fadlina Chany Saputri, and Yahdiana Harahap. "Pharmacokinetic Herb-Drug Interaction between Hibiscus sabdariffa Calyces Aqueous Extract and Captopril in Rats." Evidence-Based Complementary and Alternative Medicine 2020 (June 17, 2020): 1–8. http://dx.doi.org/10.1155/2020/5013898.

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Hibiscus sabdariffa L. (Malvaceae) is a traditional medicinal herb widely consumed as a beverage (“hibiscus tea”), and its global popularity is expanding due to health benefits such as blood pressure and cholesterol control. Previous studies showed that Hibiscus sabdariffa is coadministered with antihypertensives and antihyperlipidemics, thus predisposing herb-drug interactions. We investigated the pharmacokinetic interaction between H. sabdariffa L. aqueous extract and captopril, a frequently prescribed antihypertensive. In this study, chemical profile of H. sabdariffa L. aqueous extract was

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Stojković, Dejan, Maria Inês Dias, Danijela Drakulić, et al. "Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells." Pharmaceuticals 13, no. 4 (2020): 78. http://dx.doi.org/10.3390/ph13040078.

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Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxic

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Bednarska, Katarzyna, Piotr Kuś, and Izabela Fecka. "Investigation of the Phytochemical Composition, Antioxidant Activity, and Methylglyoxal Trapping Effect of Galega officinalis L. Herb In Vitro." Molecules 25, no. 24 (2020): 5810. http://dx.doi.org/10.3390/molecules25245810.

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Galega officinalis L. has been known for centuries as an herbal medicine used to alleviate the symptoms of diabetes, but its comprehensive chemical composition and pharmacological activity are still insufficiently known. The current study involved the qualitative and quantitative phytochemical analysis and in vitro evaluation of the antioxidative and methylglyoxal (MGO) trapping properties of galega herb. Ultra high-performance liquid chromatography coupled with both the electrospray ionization mass spectrometer and diode-array detector (UHPLC-ESI-MS and UHPLC-DAD) were used to investigate the

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Bodalska, Agnieszka, Adam Kowalczyk, and Izabela Fecka. "Stability of Rosmarinic Acid and Flavonoid Glycosides in Liquid Forms of Herbal Medicinal Products—A Preliminary Study." Pharmaceuticals 14, no. 11 (2021): 1139. http://dx.doi.org/10.3390/ph14111139.

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Peppermint leaf, sage leaf, thyme herb, and their preparations are common components of herbal medicinal products (HMPs). According to the European Pharmacopoeia guidelines, the above-mentioned plant substances are standardized for the content of essential oils, omitting polyphenols, which also have a significant impact on their activities. The aim of this study was to evaluate the stability of the predominant polyphenols—rosmarinic acid, luteolin-7-O-β-glucuronide, and eriocitrin—in selected commercial liquid HMPs containing thyme, sage, and peppermint under long-term, intermediate, and accel

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Babotă, Mihai, Oleg Frumuzachi, Andrei Mocan, et al. "Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii." Plants 11, no. 20 (2022): 2773. http://dx.doi.org/10.3390/plants11202773.

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Hypericum perforatum L., also known as St. John’s Wort, is recognized worldwide as a valuable medicinal herb; however, other Hypericum species were intensively studied for their bioactive potential. To fill the research gap that exists in the scientific literature, a comparative evaluation between H. alpigenum Kit., H. perforatum L. and H. rochelii Griseb. & Schenk was conducted in the present study. Two types of herbal preparations obtained from the aerial parts of these species were analyzed: extracts obtained through maceration and extracts obtained through magnetic-stirring-assisted ex

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Ge, Yuebin, Fei Zhang, Qin Qin, Yingying Shang, and Dingrong Wan. "In VivoEvaluation of the Antiasthmatic, Antitussive, and Expectorant Activities and Chemical Components of ThreeElaeagnusLeaves." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/428208.

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The leaf ofElaeagnus lanceolataandElaeagnus henryias well asElaeagnus pungenshas been documented as an effective herb for the treatment of asthma and chronic bronchitis in traditional clinical medicine. This study was aimed at evaluating the antiasthmatic, antitussive, and expectorant activities of the water extracts from the three plantsin vivoand analyzing their chemical components by HPLC-DAD. At the medium and high doses, the water extracts of threeElaeagnusleaves significantly prolonged the preconvulsive time (P< 0.01) in guinea pigs, lengthened the latent period of cough (P< 0.01)

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