Academic literature on the topic 'Heterologe Expression'

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Journal articles on the topic "Heterologe Expression"

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Hahn, T., L. Kilian, K. Muffler, S. Lang, and R. Ulber. "Erstmalige heterologe Expression einer Fucosidase." Chemie Ingenieur Technik 82, no. 9 (August 27, 2010): 1488. http://dx.doi.org/10.1002/cite.201050112.

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Lutz-Wahl, S., M. Wälz, M. L. Magri, K. Liebeton, J. Eck, and L. Fischer. "Heterologe Expression und Charakterisierung einer stereoselektiven Nitril-Hydratase." Chemie Ingenieur Technik 78, no. 9 (September 2006): 1399–400. http://dx.doi.org/10.1002/cite.200650178.

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Crüsemann, Max, Raphael Reher, Isabella Schamari, Alexander O. Brachmann, Tsubasa Ohbayashi, Markus Kuschak, Davide Malfacini, et al. "Heterologe Expression, Biosynthese und ökologische Funktion des selektiven Gq‐Signaltransduktionsinhibitors FR900359." Angewandte Chemie 130, no. 3 (December 20, 2017): 844–49. http://dx.doi.org/10.1002/ange.201707996.

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Richter, A., M. Müller, H. Kiesecker, and H. J. Jacobsen. "Expressionsstabilität in transgenen Erbsen – Heterologe Expression von Polygalakturonase-inhibierenden Proteinen in transgenen Erbsen." Journal für Verbraucherschutz und Lebensmittelsicherheit 1, S1 (November 2006): 132. http://dx.doi.org/10.1007/s00003-006-0090-6.

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Yan, Fu, David Auerbach, Yi Chai, Lena Keller, Qiang Tu, Stephan Hüttel, Amelie Glemser, et al. "Biosynthese und heterologe Expression der Vioprolide: rationale gentechnische Eingriffe in die Biosynthese und 4-Methylazetidincarbonsäure-Bildung." Angewandte Chemie 130, no. 28 (June 8, 2018): 8890–95. http://dx.doi.org/10.1002/ange.201802479.

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Szankowski, I., H. Li, H. Flachowsky, T. Fischer, V. Hanke, G. Forkmann, D. Treutter, T. Hoffmann, and W. Schwab. "Modifikation der Flavonoid-Biosynthese in Apfel (Malus domestica Borkh.) durch heterologe Expression des Lc-Gens aus Mais." Journal für Verbraucherschutz und Lebensmittelsicherheit 2, S1 (December 2007): 106. http://dx.doi.org/10.1007/s00003-007-0269-5.

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Elliott, Beth, Christine Richardson, Jamie Winderbaum, Jac A. Nickoloff, and Maria Jasin. "Gene Conversion Tracts from Double-Strand Break Repair in Mammalian Cells." Molecular and Cellular Biology 18, no. 1 (January 1, 1998): 93–101. http://dx.doi.org/10.1128/mcb.18.1.93.

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ABSTRACT Mammalian cells are able to repair chromosomal double-strand breaks (DSBs) both by homologous recombination and by mechanisms that require little or no homology. Although spontaneous homologous recombination is rare, DSBs will stimulate recombination by 2 to 3 orders of magnitude when homology is provided either from exogenous DNA in gene-targeting experiments or from a repeated chromosomal sequence. Using a gene-targeting assay in mouse embryonic stem cells, we now investigate the effect of heterology on recombinational repair of DSBs. Cells were cotransfected with an endonuclease expression plasmid to induce chromosomal DSBs and with substrates containing up to 1.2% heterology from which to repair the DSBs. We find that heterology decreases the efficiency of recombinational repair, with 1.2% sequence divergence resulting in an approximately sixfold reduction in recombination. Gene conversion tract lengths were examined in 80 recombinants. Relatively short gene conversion tracts were observed, with 80% of the recombinants having tracts of 58 bp or less. These results suggest that chromosome ends in mammalian cells are generally protected from extensive degradation prior to recombination. Gene conversion tracts that were long (up to 511 bp) were continuous, i.e., they contained an uninterrupted incorporation of the silent mutations. This continuity suggests that these long tracts arose from extensive degradation of the ends or from formation of heteroduplex DNA which is corrected with a strong bias in the direction of the unbroken strand.
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Hidayani, Andi Aliah, Odang Carman, and Alimuddin. "Effectiveness of B-actin promoter on driving target gene expression in common carp transgenesis." Jurnal Akuakultur Indonesia 10, no. 1 (January 1, 2011): 16. http://dx.doi.org/10.19027/jai.10.16-23.

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<p>Promoter in transgene construct plays an important role on regulating of transgene expression level in transgenic fish. In fish transgenesis, researcher convinced that use all-fish gene construct is safety and prospective. This study was performed to compare effectiveness b-actin promoter, - the promoter which has ubiquitous, constitutive, housekeeping characteristics, from common carp (homologous) and from tilapia and medaka b-actin promoters (heterologous) in driving of green fluorescent protein (GFP) expression as a model of target gene on common carp<em> </em>transgenesis. These gene constructs were separately microinjected into cytoplasm of 60 one-cell-stage common carp embryos. The results suggested that 70% survival rate at embryo stage and 45% hatching rate values showed that the microinjection was performed successfully. Percentage of embryos expressing GFP gene were slightly higher when injected using common carp and medaka promoters than those of using tilapia promoter. Percentage of larvae expressing GFP using common carp promoter was similar with medaka promoter. Furthermore, GFP expression using common carp b-actin promoter could be detected at one-week-old larvae, while GFP expressing using medaka b-actin promoter was lasted at 2-day-old larvae. The results demonstrated that homologous promoter more effective in driving of a target gene expression than that of heterologous promoter. </p> <p>Key words: homologous promoter, GFP, transgenesis, common carp</p> <p> </p> <p>ABSTRAK</p> <p>Promoter dalam konstruksi transgen berperan penting dalam pengaturan tingkat ekspresi transgen pada ikan transgenik. Dalam transgenesis ikan, peneliti meyakini bahwa penggunaan konstruksi gen "all-fish" adalah aman dan prospektif. Penelitian ini dilakukan untuk membandingkan efektivitas promoter β-aktin, - promoter yang memiliki ciri <em>ubiquitous</em>, <em>constitutive</em>, dan <em>housekeeping</em>, dari ikan dari ikan mas (homolog) dan ikan nila dan ikan medaka (heterolog) dalam mengendalikan ekspresi gen GFP sebagai model gen pada transgenesis ikan mas. Setiap konstruksi gen tersebut diinjeksikan secara terpisah ke sitoplasma embrio ikan mas fase 1 sel sebanyak 60 embrio. Hasil penelitian dengan kelangsungan hidup embrio 70% dan derajat penetasan 45% menunjukkan bahwa kegiatan mikroinjeksi berhasil dengan baik. Persentase embrio mengekspresikan gen GFP yang diinjeksi konstruksi gen dengan promoter β-aktin ikan mas dan ikan medaka sedikit lebih tinggi dibandingkan dengan yang menggunakan promoter β-aktin ikan nila. Selanjutnya, ekspresi gen GFP yang dikendalikan oleh promoter β-aktin ikan mas dapat dideteksi pada larva berumur 1 minggu, sedangkan ekspresi GFP dengan promoter β-aktin ikan medaka hanya bisa terdeteksi hingga larva berumur 2 hari. Hasil penelitian menunjukkan bahwa promoter homolog adalah lebih efektif dalam mengatur ekspresi gen target dibandingkan dengan promoter heterolog.</p> <p>Kata kunci: promoter homolog, GFP, transgenesis, ikan mas</p>
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Mérida-Floriano, Angela, Will P. M. Rowe, and Josep Casadesús. "Genome-Wide Identification and Expression Analysis of SOS Response Genes in Salmonella enterica Serovar Typhimurium." Cells 10, no. 4 (April 19, 2021): 943. http://dx.doi.org/10.3390/cells10040943.

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A bioinformatic search for LexA boxes, combined with transcriptomic detection of loci responsive to DNA damage, identified 48 members of the SOS regulon in the genome of Salmonella enterica serovar Typhimurium. Single cell analysis using fluorescent fusions revealed that heterogeneous expression is a common trait of SOS response genes, with formation of SOSOFF and SOSON subpopulations. Phenotypic cell variants formed in the absence of external DNA damage show gene expression patterns that are mainly determined by the position and the heterology index of the LexA box. SOS induction upon DNA damage produces SOSOFF and SOSON subpopulations that contain live and dead cells. The nature and concentration of the DNA damaging agent and the time of exposure are major factors that influence the population structure upon SOS induction. An analogy can thus be drawn between the SOS response and other bacterial stress responses that produce phenotypic cell variants.
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Deng, Yong-Sheng, Fan-Ying Kong, Bin Zhou, Song Zhang, Meng-Meng Yue, and Qing-Wei Meng. "Heterology expression of the tomato LeLhcb2 gene confers elevated tolerance to chilling stress in transgenic tobacco." Plant Physiology and Biochemistry 80 (July 2014): 318–27. http://dx.doi.org/10.1016/j.plaphy.2014.04.017.

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Dissertations / Theses on the topic "Heterologe Expression"

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Bolliger, Gabriella. "Heterologe Expression einer funktionellen Serin-Palmitoyltransferase." Zürich : ETH, Eidgenössische Technische Hochschule Zürich, Departement Chemie und Angewandte Biowissenschaften, 2005. http://e-collection.ethbib.ethz.ch/show?type=dipl&nr=176.

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Toepfer, Cornelia. "Heterologe Expression einer funktionellen Domäne des nikotinischen Acetylcholinrezeptors." [S.l. : s.n.], 2002. http://www.diss.fu-berlin.de/2002/203/index.html.

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Höffle, Anja. "Ektopische Expression schwer exprimierbarer nikotinischer Acetylcholinrezeptoren in Zellinien." [S.l.] : [s.n.], 2001. http://ArchiMeD.uni-mainz.de/pub/2002/0037/diss.pdf.

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Janzik, Ingar. "Heterologe Expression und Charakterisierung von Subtilasen aus Lycopersicon esculentum /." [S.l.] : [s.n.], 2000. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=13658.

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Kelle, Sebastian [Verfasser]. "Heterologe Expression von Enzymen aus Basidiomyceten in Pichia pastoris / Sebastian Kelle." Hannover : Technische Informationsbibliothek (TIB), 2015. http://d-nb.info/1084239795/34.

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Pechlivanis, Ioannis. "Heterologe Expression des AAA-Proteins Pex6p in der Hefe Saccharomyces cerevisiae." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=975790919.

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Scheltz, Tineke [Verfasser]. "Dictyostelium-Amöben : Charakterisierung endogener und heterologe Expression humaner Tetraspanine / Tineke Scheltz." Kiel : Universitätsbibliothek Kiel, 2017. http://d-nb.info/1129685403/34.

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Pfeffer, Jan Christoph. "The lipases from Candida antarctica cloning, expression and their application in the synthesis of structured lipids /." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-34375.

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Büchi, Ruth. "Charakterisierung und heterologe Expression des citratbindenden Proteins aus Lutoiden von Hevea brasiliensis /." [S.l.] : [s.n.], 1997. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=12402.

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Galperin, Ilya [Verfasser]. "Das ligninolytische System von Pleurotus sapidus: Transkriptomanalyse und heterologe Expression einer Arylalkoholoxidase / Ilya Galperin." Gießen : Universitätsbibliothek, 2018. http://d-nb.info/1162053968/34.

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Books on the topic "Heterologe Expression"

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Radzio, Renate. Heterologe Genexpression in dem Cephalosporin C produzierenden Hyphenpilz Acremonium chrysogenum. Berlin: J. Cramer, 1997.

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Schründer, Jürgen. Cytoplasmatische Vektoren zur heterologen Genexpression bei Hefen: Konstruktion und Analyse linearer Hybridplasmide basierend auf dem Killer-System der Hefe Kluyveromyces lactis. Berlin: J. Cramer, 1996.

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Heterologe Gene Expression in Dem Cephalosporin C Produzierenden Hyphenpilz Acremonium Chrysogenum (Bibliotheca Mycologica). Gebruder Borntraeger Verlagsbuchhandlung, 1997.

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Yang, Yea-Huey. Cloning, heterologus expression, and characterization of the cytochrome P450 monooxygenases in rainbow trout (Oncorhynchus mykiss). 1997.

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Cytoplasmatische Vektoren Zur Heterologen Genexpression Bei Hefen: Konstruktion Und Analyse Linearer Hybridplasmide Basierend Auf Dem Killer - System (Bibliotheca Mycologica). Gebruder Borntraeger Verlagsbuchhandlung, 1996.

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Book chapters on the topic "Heterologe Expression"

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Glatt, H. R., A. Czich, I. Bartsch, J. L. Falany, and C. N. Falany. "Heterologe Expression von Sulfotransferasen: Illustration eines neuen Forschungsansatzes in der Toxikologie." In Ersatz- und Ergänzungsmethoden zu Tierversuchen, 72–79. Vienna: Springer Vienna, 1995. http://dx.doi.org/10.1007/978-3-7091-9418-8_11.

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Lecoeur, Sylvaine, Jean-Charles Gautier, Claire Belloc, Aline Gauffre, and Philippe H. Beaune. "[8] Use of heterologus expression systems to study autoimmune drug-induced hepatitis." In Methods in Enzymology, 76–85. Elsevier, 1996. http://dx.doi.org/10.1016/s0076-6879(96)72010-4.

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