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Dissertations / Theses on the topic 'Heterologous expression in plant'

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Published: 4 June 2021
Last updated: 1 February 2022

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1

Lilley, Catherine Jane. "Heterologous expression from Agrobacterium virulence promoters." Thesis, Durham University, 1991. http://etheses.dur.ac.uk/6202/.

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The aim of this work was twofold: to construct plasmids with a gene encoding a pesticidal protein expressed from an Agrobacterium tumefaciens virulence promoter and to determine, in planta, the sites of Agrobacterium vir-induction. A number of methods were employed to detect in situ vir-induction and, to this end, genes encoding β-glucuronidase (GUS) and bioluminescence (lux) were linked in plasmid constructs to Agrobacterium vir-promoters. In each case, expression of the gene was shown to be induced by the v/r-inducing phenolic compound acetosyringone. An existing plasmid, in which the lacZ g
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2

Raemaekers, Romaan J. M. "Expression of functional plant lectins in heterologous systems." Thesis, Durham University, 2000. http://etheses.dur.ac.uk/4621/.

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The mannose-binding lectin from snowdrop (Galanthus nivalis agglutinin; GNA) was produced in Escherichia coli and purified as a functional protein after denturation/renaturation. Incorporation of the four extra C-terminal residues recently revealed from X-ray crystallographic data demonstrated that these residues increase binding to the glycoprotein carboxypeptidase Y. However, no differences in activities were observed in haemagglutination assays when compared to native GNA and toxicity towards rice brown planthopper (Nilaparvata lugens', BPH) in artificial diet bioassays was unaltered. Site-
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3

Scott, R. "Expression of aminoglycoside phosphotransferase II in heterologous cells." Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356036.

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4

Duarte, João Gomes de Matos. "Relatório integrador da actividade profissional." Master's thesis, ISA, 2013. http://hdl.handle.net/10400.5/6469.

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Mestrado em Engenharia Agronómica - Proteção das Plantas - Instituto Superior de Agronomia<br>This thesis constitutes a summary of my work for the past eight years. Given the different subjects that I have been involved, I selected three major lines of work to expose in this thesis, which are subdivided in four Chapters: Chapter I: “Descriptive Curriculum vitae” containing a brief presentation of the work developed, constituting a more descriptive cv, with a clear description of the projects I have been involved, techniques that I have become acquainted and a brief explanation of my responsibi
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5

Martin, David John. "Studies into the regulation and expression of Z. mays Ac in heterologous plant species." Thesis, University of Leicester, 1997. http://hdl.handle.net/2381/29755.

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The Z. mays Ac/Ds transposable elements have been introduced into a number of heterologous plant species, where they have been employed as genomic mutagens in transposon-tagging strategies. Although successful, many strategies fail to regulate the expression of the controlling Ac element, therefore allowing Ds transposition to occur throughout the plant life cycle. This can generate genetically mosaic organisms whose progeny may contain unique or common Ds transposition events. In an attempt to develop a controlled Ac/Ds transposon-tagging strategy, this thesis examined whether an anther-speci
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6

Olsen, Rebecca Lynn. "Modification of plant and yeast lipids by heterologous expression of protist, algal, and animal desaturases." Online access for everyone, 2006. http://www.dissertations.wsu.edu/Dissertations/Fall2006/r_olsen_011907.pdf.

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7

Ali, Zahid. "Developing strategies for homologous, heterologous plant expression system for physiological investigations of respective target proteins." kostenfrei, 2007. http://d-nb.info/987031201/34.

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8

Bailey, Keir. "Investigating the heterologous expression of plant secondary metabolic enzymes to produce curcuminoids and curcuminoid derivatives." Thesis, University of York, 2016. http://etheses.whiterose.ac.uk/16284/.

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Curcuminoids are highly bioactive polyketide chemicals produced in the rhizomes of the turmeric plant (Curcuma longa). In addition to giving turmeric powder its distinctive yellow colour and flavour, these molecules elicit a plethora of medicinal effects. This project aimed to biosynthesize curcuminoids and their derivatives using the metabolic engineering of Baker’s yeast (Saccharomyces cervisiae) and tobacco (Nicotiana benthamiana). Phenylpropanoids, sustainably derived from bio-refinery waste, were to be used as starting materials. To convert phenylpropanoids into curcuminoids three enzymat
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9

Barbaro, Maria Raffaella <1985&gt. "The Domon Family of Plant Plasma Membrane B-Type Cytochromes: Heterologous Expression, Biochemical Characterization and Physiological Roles in Vivo." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4713/.

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The DOMON domain is a domain widespread in nature, predicted to fold in a β-sandwich structure. In plants, AIR12 is constituted by a single DOMON domain located in the apoplastic space and is GPI-modified for anchoring to the plasma membrane. Arabidopsis thaliana AIR12 has been heterologously expressed as a recombinant protein (recAtAIR12) in Pichia pastoris. Spectrophotometrical analysis of the purified protein showed that recAtAir12 is a cytochrome b. RecAtAIR12 is highly glycosylated, it is reduced by ascorbate, superoxide and naftoquinones, oxidised by monodehydroascorbate and oxygen and i
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10

Vaishali, Mulangi Gopala Reddy. "Characterization of Polyamine Transporters from Rice and Arabidopsis." Bowling Green State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1303231265.

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11

Sonawala, Unnati Subhash. "Understanding the role of host amino acid transporters in nutrient acquisition by oomycete pathogens." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/102868.

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Hyaloperonospora arabidopsidis (Hpa) is a naturally occurring oomycete pathogen on Arabidopsis thaliana. It is related to downy mildews of economically important crops such as cabbage, kale and broccoli, belonging to the Brassicaceae family. Downy mildew pathogens are obligate biotrophs that extract nutrients exclusively from living plant cells. As a part of its obligate biotrophy lifestyle, Hpa has lost the ability to assimilate inorganic nitrogen and sulfur. It thus has to acquire these nutrients from the host in an organic form; possibly amino acids. Using a reverse genetic approach, I was
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12

Bartonjo, Jane Jeruto. "Heterologous Expression of Arabidopsis Thaliana Purple Acid Phosphatase Gene (Atpap15) in Crops for Phytoremediation of Sites Contaminated with Excess Phosphorus." TopSCHOLAR®, 2015. http://digitalcommons.wku.edu/theses/1505.

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AtPAP15 is one of the purple acid phosphatases expressed by Arabidopsis thaliana that has been extensively studied. Purified AtPAP15 has been shown to exhibit both phytase and phosphomonoesterase activities in acidic pH with maximal activity at pH 4.5. AtPAP15 is a phosphorus starvation inducible (PSI) gene that is expressed highly during phosphorus deficient conditions. In the current study, AtPAP15 was overexpressed in Nicotiana tabaccum under cauliflower mosaic virus (CaMV35S) constitutive promoter. After PCR confirmation of the gene, plants were transferred to the greenhouse and allowed to
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13

Wamucho, Anye, Deborah Hayford, and Cecelia A. McIntosh. "Heterologous Expression of Grapefruit Clones PGT3 and PGT9 in Yeast and Screening of Recombinant Protein for Activity." Digital Commons @ East Tennessee State University, 2012. https://dc.etsu.edu/etsu-works/336.

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The wide diversity of plant secondary products results from different modifications undergone during biosynthesis, including glucosylation. These modification reactions result in production of the compounds actually found in plants and to unique chemical and biochemical properties, including some bitter compounds in grapefruit. While the presence of a PSPG box motif allows for identification of a clone as a putative glucosyltransferase (PGT), diversity of GT primary structures makes it difficult to accurately assign specific function. Our approach is to identify and isolate putative GT clones,
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14

Mishler-Elmore, John William. "Expression Profiling and Recombinant Production of TomEP, a Tomato Extensin Peroxidase." Ohio University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1585732848912595.

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15

Loftis, Peri, Bruce Williams, Devaiah P. Shivakumar, and Cecelia A. McIntosh. "Heterologous Expression and Characterization of Putative Secondary Product Glucosyltransferase (PGT)Clones 4 and 11 Isolated from Citrus paradisi." Digital Commons @ East Tennessee State University, 2013. https://dc.etsu.edu/etsu-works/341.

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Plant secondary products such as flavonoids have a variety of roles in plants including UV protection, antifeedant activity, pollinator attraction, stress response, flavor, and many more. These compounds also have effects on human physiology. Glucosylation is an important modification of many flavonoids and other plant secondary products. In grapefruit, glucosylation is important in the synthesis of the bitter compound naringin and several flavonoid glucosyltransferase (GT) enzymes have been characterized from young grapefruit leaf tissue. To study structure and function of flavonoid GTs, it i
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16

Ngoepe, Mafora Gloria. "Heterologous expression of a Mukwa (pterocarpus angolensis ) seed lectin (Pal) gene in Escherichia coli, Saccharomyces cerevisiae and Yarrowia lipolytica and construction of Pal recombinant vector for expression in Aspergillus niger." Thesis, University of Limpopo (Turfloop Campus), 2011. http://hdl.handle.net/10386/857.

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Thesis (M.Sc. (Microbiology)) --University of Limpopo, 2011<br>Pterocarpus angolensis seed lectin (PAL), a 28 kDa non glycosylated protein, was initially successfully cloned and expressed in E. coli for ease of high protein production. It was discovered, however, as in similar studies that the recombinant PAL yield in E. coli is low and localized intracellularly. This makes extraction even more difficult because most of the protein is lost either when the cell undergoes lysing or when there is incomplete extraction. As a result of the low yields in E. coli, expression vectors were constructed
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17

Meng, Meng. "Plant UDP-glucose Pyrophosphorylase : Function and Regulation." Doctoral thesis, Umeå : Department of Plant Physiology, Umeå University, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1796.

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18

Karamat, Fazeelat. "Identification and functional characterization of the first two aromatic prenyltransferases implicated in the biosynthesis of furanocoumarins and prenylated coumarins in two plant families : Rutaceae and Apiaceae." Thesis, Université de Lorraine, 2013. http://www.theses.fr/2013LORR0029/document.

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Les furocoumarines constituent l'une des classes de métabolites secondaires dérivant de la voie de biosynthèse des phénylpropanoïdes. Elles ont été décrites comme étant des phytoaléxines mais sont également très largement utilisées par l'Homme pour leurs propriétés thérapeutiques. Un certain nombre d'études biochimiques ont été réalisées afin d'en comprendre la biosynthèse mais peu de choses sont connues concernant leur déterminisme moléculaire. Dans cette étude, nous nous sommes concentrés sur la caractérisation fonctionnelle de gènes appartenant aux prényltransférases aromatiques potentielle
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19

Karaki, Lamis. "Diversité génétique et fonctionnelle des molécules homologues de PA1b chez Medicago truncatula Gaertn. ainsi qu’au sein de légumineuses originaires du Liban." Thesis, Lyon, INSA, 2013. http://www.theses.fr/2013ISAL0156/document.

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Les peptides Albumines 1 b sont des membres de la famille structurale des knottines et présentent un potentiel intéressant en tant que composés insecticides. À ce jour, leur diversité parmi les Fabacées a été essentiellement étudiée en utilisant des approches biochimiques et moléculaires. Les ressources bioinformatiques (le séquençage complet du génome, les bases de données transcriptomiques (EST…), les atlas d’expression...) de l’espèce modèle des légumineuses, Medicago truncatula Gaertn. (Mtr), nous a permis de développer une approche génomique de cette biodiversité. Deux objectifs principau
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20

Malumpong, Chanate. "Characterising the DMC1 and RAD51 strand-exchange genes from rice (Oryza sativa L.) through in planta inactivation of heterologous expression in Arabidopsis thaliana." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.508208.

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21

Lubini, Greice. "Caracterização do Gene NtCDKG;2 Expresso no Pistilo de Nicotiana tabacum L." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-22042013-141248/.

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A biologia da reprodução sexual de plantas é um campo de pesquisa de grande importância, já que a maioria dos alimentos consumidos pelo homem é composta de partes reprodutivas das plantas (frutos e sementes), oriundas do desenvolvimento de partes do pistilo fertilizado. Em Nicotiana tabacum, identificou-se um gene específico de estigma/estilete, SCI1 (Stigma/style Cell-cycle Inhibitor 1), que atua na inibição da proliferação celular (DePaoli et al., 2011). Através de ensaios de pull-down, verificou-se a interação da proteína SCI1 com uma proteína quinase dependente de ciclina (CDK) (Strin
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22

Gaspar, Paulo Miguel da Silva. "Gene optimization for heterologous expression." Master's thesis, Universidade de Aveiro, 2010. http://hdl.handle.net/10773/7238.

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Mestrado em Engenharia de Computadores e Telemática<br>Com o uso de computadores para assistir investigadores na área da biologia na resolução de tarefas complexas, o seu potencial surgiu como uma ajuda preciosa para alcançar o que está para além das capacidades humanas. Para um biólogo, nos tempos que correm, lidar com um computador é uma tarefa tão trivial como realizar experiencias em laboratório. Assim, a capacidade fornecida pela tecnologia computacional, juntamente com as centenas de aplicações e ferramentas de software que já existem, concedem à Biologia um apoio significativo pa
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23

Kaewthai, Nomchit. "In vitro and in vivo approaches in the characterization of XTH gene products." Doctoral thesis, KTH, Glykovetenskap, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-28222.

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ABSTRACT The xyloglucan endo-transglycosylase/hydrolase (XTH) genes are found in all vascular and some nonvascular plants. The XTH genes encode proteins which comprise a subfamily of glycoside hydrolase (GH) family 16 in the Carbohydrate-Active enZYmes (CAZY) classification. The XTH gene products are believed to play intrinsic role in cell wall modification during growth and development throughout the lifetime of the plant. In the present investigation, biochemical and reverse genetic approaches were used to better understand the functions of individual members of the XTH gene family of two im
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Richards, Kevin Stephen. "Insect expression systems : improving intracellular and regulated heterologous gene expression." Thesis, Oxford Brookes University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.407367.

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25

Krezdorn, Christian. "Heterologous expression of human glycosyltransferases in yeast /." [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10372.

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26

Hills, David. "Heterologous expression of basic fibroblast growth factor." Thesis, University of Portsmouth, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304472.

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27

Mielke, Tamara. "Heterologous expression and characterisation of unspecific peroxygenases." Thesis, University of York, 2016. http://etheses.whiterose.ac.uk/20015/.

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In 2004, unspecific peroxygenases (UPOs) from fungi were first identified and showed to catalyse selective oxygenation reactions, with high turnovers, good stability and a broad reaction scope, providing a valuable alternative to established biocatalytic hydroxylation systems such as cytochrome P450s. At the start of this project, access to UPOs was limited to expression in their native fungi, hence studies were performed looking into heterologous expression of the enzymes from Agrocybe aegerita and Agaricus bisporus, followed by characterisation, and application of the enzymes.
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Vauclare, Pierre. "Structure, biogenèse et expression de la protéine T du complexe de la glycine décarboxylase des plantes supérieures." Grenoble 1, 1996. http://www.theses.fr/1996GRE10051.

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Au cours de ce travail de these nous avons caracterise un adnc correspondant a la proteine t (1560 pb) du complexe de la glycine decarboxylase. Il possede un cadre de lecture ouvert de 1220 pb codant pour un peptide mature de 378 acides amines. La sequence primaire ainsi que la masse deduite (40 961 da) ont ete confirme respectivement par microsequencage et par mesure au spectrometre de masse. Dans la perspective d'une etude structurale, nous avons reussi a surexprimer chez e. Coli la proteine t en construisant un vecteur d'expression produisant un arnt rare chez les bacteries. L'etude de l'ex
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Montero, Mirabet Maria. "Evaluación de los efectos no intencionados de los transgenes en plantas modificadas genéticamente (MG) resistentes a plagas y diseñadas como biofactorías de péptidos antimicrobianos." Doctoral thesis, Universitat de Girona, 2012. http://hdl.handle.net/10803/97166.

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Genetically modified crops are submitted to strict regulation to ensure the safety of consumers and the environment. To complement the comparison between GM plants and their counterparts, in the present Thesis, we evaluated the possible unexpected effects of the transgene on the host plant, by means of transcriptomic technologies. More exactly, we studied three pathogen-resistant GM rice lines: S-afp, expressing constitutively the antifungal protein AFP; and S-bp217 and S-bp213, expressing undecapeptide BP100 derivatives, which were developed in the UdG in the context of this Thesis. Although
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Shephard, Freya. "cDNA cloning and heterologous expression of diterpene synthases." Thesis, University of Bristol, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.251559.

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31

Buck, Justin David. "Physiological effects of heterologous expression of proteorhodopsin photosystems." Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/71464.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, 2012.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 175-195).<br>Proteorhodopsin (PR) phototrophy plays an important role in the marine ecosystem, harvesting energy from sunlight for a diverse community of hetertrophic organisms. The simple proteorhodopsin photosystem (PRPS) composed of six to seven genes is sufficient for producing a functional light-driven proton pump, capable of powering cellular processes. This thesis describes the functional characterization of a
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Yang, Xiao-Ming. "Heterologous expression and characterization of a human bifunctional enzyme." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41792.

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Human mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase was expressed in a soluble and active form in Escherichia coli, purified to homogeneity for the first time and characterized. Phosphate is absolutely required for the dehydrogenase activity. NADP can be utilized by the dehydrogenase with a low turnover rate. The properties of the enzyme suggest that it has evolved from NADP to NAD specificity by the unique mechanism of substituting inorganic phosphate for the 2$ sp prime$-phosphate of NADP. The role of Mg$ sp{++}$ in this process i
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Sinclair, Graham Bernard. "Mutation analysis, heterologous expression, and characterization of human glucocerebrosidase." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/NQ62529.pdf.

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McCarroll, Lynn. "Characterisation of heterologous gene expression in insect cell lines." Thesis, Oxford Brookes University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389533.

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Jones, S. "Cloning and expression of heterologous genes in Lactobacillus plantarum." Thesis, Cranfield University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305285.

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Gash, Stephen P. "Expression of heterologous genes in sporulating Bacillus thuringiensis cells." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360633.

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Voulgari, Athina. "Studies on transmissible spongiform encephalopathies : heterologous expression of PrP." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/14616.

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To investigate the role of PrP in conferring susceptibility to transmissible spongiform encephalopathies and furthermore to investigate the effect of restricted hamster PrP expression on the susceptibility to the hamster scrapie agent, we attempted to generate transgenic mice by using the hamster PrP open reading frame only, linked to appropriate regulatory 5' flanking sequences of brain specific genes. Although correct mRNA expression was achieved in some of the transgenic lines generated, problems were encountered with PrP protein production, indicating translational control of PrP. It was p
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Gong, Fangcheng. "Probing the plant endomembrane-secretory pathway using heterologous membrane protein markers." Diss., The University of Arizona, 1995. http://hdl.handle.net/10150/187119.

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Using gene expression systems developed in Nicotiana tabacum, the transport of various heterologous membrane proteins (SYNVG, IBV M, VSVG, and their derivatives) was studied within the plant secretory pathway. The results of these experiments indicated that the proteins accumulated in various endomembrane compartments of plant cells, but not in those counterparts observed in their native hosts. These results suggest that membrane proteins of mammalian and plant cells share a well-conserved mechanism for ER targeting and translocation, but differences exist in their machinery for retention and
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O'Dwyer, Cliona Anne. "Expression and vaccine formulation of heterologous antigens using commensal Neisseria." Thesis, Imperial College London, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.401669.

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Carrier, David John. "The Arabidopsis Auxin Transporter Aux1 : Heterologous Expression, Characterisation and Purification." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.523129.

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Lloyd, John S. "Heterologous expression and site-directed mutagenesis of soluble methane monooxygenase." Thesis, University of Warwick, 1997. http://wrap.warwick.ac.uk/59600/.

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The purpose of this investigation was to study the heterologous expression of soluble methane monooxygenase (sMMO) genes from Methylococcus capsulatus (Bath) and Methylosinus trichosporium OB3b. Using the T7-RNA polymerase expression system, the entire sMMO operon and subclones (constructed using the polymerase chain reaction) were over-expressed in E. coli. Results obtained using the Me. capsulatus (Bath) sMMO operon confirmed previous reports (C. West, G. P. C. Salmond, H. Dalton and 1. C. Murrell (1992). J Gen. Microbial. 138, 1301-1307) that functional expression of protein B and the reduc
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Chitpinityol, Supannee. "Heterologous expression and site-directed mutagenesis of the enzyme chymosin." Thesis, University of Reading, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320101.

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Ekinci, Mehmet Sait. "Heterologous expression of genes in the anaerobic bacterium Streptococcus bovis." Thesis, University of Aberdeen, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387523.

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The main objective of this work was to investigate the expression of xylanase and cellulose genes from <I>Ruminococcus flavefaciens</I> when introduced into related Gram-positive bacteria including the rumen bacterium <I>Streptococcus bovis</I>. In addition the discovery, and isolation of the β(1,3-1,4)-glucanase gene of <I>S. bovis</I> in the course of this work may provide new possibilities to express foreign genes in <I>S. bovis</I> or in other Gram-positive bacteria. The main findings of this work are summarised below: 1. Genes encoding polysaccharidase activity from the strictly anaerobic
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Shwed, Philip S. (Philip Steven) Carleton University Dissertation Biology. "A study of heterologous expression of Xenopus laevis histone H5." Ottawa, 1991.

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Rahman, Md Mahbubar, Jay Shockey, and Aruna Kilaru. "Identification, and Heterologous Expression Analysis of Avocado DGAT1 and DGAT2." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etsu-works/4796.

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The neutral lipid triacylglycerol (TAG) is the main storage lipid in plants. When stored in seeds, TAG provides the carbon and energy source during germination. There is significant human nutritional demand for vegetable oil, but its use in production of renewable biomaterials and fuels has intensified the need to increase oil production. In plants, the final and committed step in TAG biosynthesis is catalyzed by diacylglycerol acyltransferases (DGAT) and/or a phospholipid: diacylglycerol acyltransferases (PDAT). Both DGAT and PDAT contribute to seed TAG biosynthesis in an independent or overl
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Kirikyali, Narin. "Heterologous expression and characterisation of [beta]-xylosidases from fungal sources." Thesis, University of Nottingham, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.716838.

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Beta-xylosidases (E.C. 3.2.1.37) are a subclass of glycoside hydrolases (E C. 3.2.1.-) which hydrolyses the pi,4 glycosidic linkages of short chain xylo- oligosaccharides and xylobiose from the non-reducing termini releasing xylose monomers. They constitute a group of enzymes that have the biotechnological potential use in various industrial processes. As a consequence it has been a necessity for enzymes involved in hemicellulose hydrolysis, of industrial interest, to be individually identified and characterised in order to utilise them for practical purposes. In order to expand the range of a
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Wynant, Inneke. "Heterologous expression systems for metabolite production during early drug research." Doctoral thesis, Universite Libre de Bruxelles, 2010. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210070.

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La bio-transformation naturelle des médicaments peut produire des métabolites toxiques; l’identification de ces métabolites est essentielle dans la stratégie de choix de molécules thérapeutiques. En appliquant les technologies de fermentation en bioréacteur des cellules hétérologues (souches d’E. coli recombinantes exprimant une iso-enzyme de cytochrome P450 humain avec la réductase humain), la bioconversion du substrat (principe actif) en ses métabolites de dégradation, a été réalisée à grande échelle (&<br>Doctorat en Sciences<br>info:eu-repo/semantics/nonPublished
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48

Jordan, Bernadette. "Factors influencing heterologous gene expression in the yeast Saccharomyces cerevisiae." Thesis, University of Leicester, 1993. http://hdl.handle.net/2381/34432.

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The yeast Saccharomyces cerevisiae is widely employed as a host for the production of recombinant proteins. In the work presented here, expression of recombinant reporter proteins has been analysed in yeast, with the aim of determining factors that contribute to the expression performance of recombinant genes. Initially, expression of bacterial aminoglycoside phosphotransferase was analysed in yeast. This heterologous gene showed very low expression levels, resulting from a small decrease in mRNA abundance, plus a large decrease in translation efficiency. This appeared to be due to an unfavour
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49

Flores, Ortiz Carlos Alberto. "Investigating the functionality of papaver s-determinants in highly diverged heterologous systems." Thesis, University of Birmingham, 2015. http://etheses.bham.ac.uk//id/eprint/6324/.

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Self-incompatibility (SI) is an important genetic mechanism that angiosperms utilize to reject "self' pollen. In Papaver rhoeas (poppy) Sl is controlled in an allele-specific manner by a single locus with multiple haplotypes; each haplotype encodes male (PrpS) and female (PrsS) S-determinants. PrsS-PrpS interaction triggers Sl, stimulating cation channel activity and increases in cytosolic free Ca2+ ([Ca2+]i), triggering a signalling network involving actin cytoskeleton alterations and programmed cell death (PCD). PrpS was recently functionally transferred into self-compatible, highly diverged
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50

Gu, Lina. "Heterologous expression of manganese peroxidase from Phanerochaete chrysosporium in Pichia pastoris." Related Electronic Resource: Current Research at SU : database of SU dissertations, recent titles available full text, 2003. http://wwwlib.umi.com/cr/syr/main.

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