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Dissertations / Theses on the topic 'Heterologous expression'

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Published: 4 June 2021

Last updated: 26 July 2025

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1

Gaspar, Paulo Miguel da Silva. "Gene optimization for heterologous expression." Master's thesis, Universidade de Aveiro, 2010. http://hdl.handle.net/10773/7238.

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Mestrado em Engenharia de Computadores e Telemática<br>Com o uso de computadores para assistir investigadores na área da biologia na resolução de tarefas complexas, o seu potencial surgiu como uma ajuda preciosa para alcançar o que está para além das capacidades humanas. Para um biólogo, nos tempos que correm, lidar com um computador é uma tarefa tão trivial como realizar experiencias em laboratório. Assim, a capacidade fornecida pela tecnologia computacional, juntamente com as centenas de aplicações e ferramentas de software que já existem, concedem à Biologia um apoio significativo pa

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2

McCracken, Andrea. "Heterologous gene expression in Lactobacillus." Thesis, Queensland University of Technology, 1998.

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3

Lilley, Catherine Jane. "Heterologous expression from Agrobacterium virulence promoters." Thesis, Durham University, 1991. http://etheses.dur.ac.uk/6202/.

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The aim of this work was twofold: to construct plasmids with a gene encoding a pesticidal protein expressed from an Agrobacterium tumefaciens virulence promoter and to determine, in planta, the sites of Agrobacterium vir-induction. A number of methods were employed to detect in situ vir-induction and, to this end, genes encoding β-glucuronidase (GUS) and bioluminescence (lux) were linked in plasmid constructs to Agrobacterium vir-promoters. In each case, expression of the gene was shown to be induced by the v/r-inducing phenolic compound acetosyringone. An existing plasmid, in which the lacZ g

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4

Richards, Kevin Stephen. "Insect expression systems : improving intracellular and regulated heterologous gene expression." Thesis, Oxford Brookes University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.407367.

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5

Krezdorn, Christian. "Heterologous expression of human glycosyltransferases in yeast /." [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10372.

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6

Hills, David. "Heterologous expression of basic fibroblast growth factor." Thesis, University of Portsmouth, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304472.

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7

Mielke, Tamara. "Heterologous expression and characterisation of unspecific peroxygenases." Thesis, University of York, 2016. http://etheses.whiterose.ac.uk/20015/.

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In 2004, unspecific peroxygenases (UPOs) from fungi were first identified and showed to catalyse selective oxygenation reactions, with high turnovers, good stability and a broad reaction scope, providing a valuable alternative to established biocatalytic hydroxylation systems such as cytochrome P450s. At the start of this project, access to UPOs was limited to expression in their native fungi, hence studies were performed looking into heterologous expression of the enzymes from Agrocybe aegerita and Agaricus bisporus, followed by characterisation, and application of the enzymes.

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8

CIVIERO, Laura. "Heterologous expression of three enzymes forstructural studies." Doctoral thesis, Università degli Studi di Verona, 2010. http://hdl.handle.net/11562/344018.

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In questo lavoro di tesi ci si è occupati dell’espressione, della purificazione e della cristallizzazione di tre enzimi (bile acid CoA:amino acid N-acyltransferase, BAAT; farnesyl cysteine-carboxyl methyltransferase, Ste14; e stearoyl-CoA desaturase, SCD) con lo scopo finale di determinarne la struttura tridimensionale mediante analisi di diffrazione di raggi X. I cDNA delle proteine in questione sono stati clonati in vettori per l’espressione in sistemi eterologhi. Le scelta del sistema di espressione opportuno per la produzione su larga scala è stata condotta dopo una valutazione dell

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9

Scott, R. "Expression of aminoglycoside phosphotransferase II in heterologous cells." Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356036.

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10

Raemaekers, Romaan J. M. "Expression of functional plant lectins in heterologous systems." Thesis, Durham University, 2000. http://etheses.dur.ac.uk/4621/.

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The mannose-binding lectin from snowdrop (Galanthus nivalis agglutinin; GNA) was produced in Escherichia coli and purified as a functional protein after denturation/renaturation. Incorporation of the four extra C-terminal residues recently revealed from X-ray crystallographic data demonstrated that these residues increase binding to the glycoprotein carboxypeptidase Y. However, no differences in activities were observed in haemagglutination assays when compared to native GNA and toxicity towards rice brown planthopper (Nilaparvata lugens', BPH) in artificial diet bioassays was unaltered. Site-

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11

Shephard, Freya. "cDNA cloning and heterologous expression of diterpene synthases." Thesis, University of Bristol, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.251559.

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12

Buck, Justin David. "Physiological effects of heterologous expression of proteorhodopsin photosystems." Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/71464.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, 2012.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 175-195).<br>Proteorhodopsin (PR) phototrophy plays an important role in the marine ecosystem, harvesting energy from sunlight for a diverse community of hetertrophic organisms. The simple proteorhodopsin photosystem (PRPS) composed of six to seven genes is sufficient for producing a functional light-driven proton pump, capable of powering cellular processes. This thesis describes the functional characterization of a

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13

Sinclair, Graham Bernard. "Mutation analysis, heterologous expression, and characterization of human glucocerebrosidase." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/NQ62529.pdf.

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14

Yang, Xiao-Ming. "Heterologous expression and characterization of a human bifunctional enzyme." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41792.

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Human mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase was expressed in a soluble and active form in Escherichia coli, purified to homogeneity for the first time and characterized. Phosphate is absolutely required for the dehydrogenase activity. NADP can be utilized by the dehydrogenase with a low turnover rate. The properties of the enzyme suggest that it has evolved from NADP to NAD specificity by the unique mechanism of substituting inorganic phosphate for the 2$ sp prime$-phosphate of NADP. The role of Mg$ sp{++}$ in this process i

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15

Gash, Stephen P. "Expression of heterologous genes in sporulating Bacillus thuringiensis cells." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360633.

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16

McCarroll, Lynn. "Characterisation of heterologous gene expression in insect cell lines." Thesis, Oxford Brookes University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389533.

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17

Jones, S. "Cloning and expression of heterologous genes in Lactobacillus plantarum." Thesis, Cranfield University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305285.

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18

Voulgari, Athina. "Studies on transmissible spongiform encephalopathies : heterologous expression of PrP." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/14616.

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To investigate the role of PrP in conferring susceptibility to transmissible spongiform encephalopathies and furthermore to investigate the effect of restricted hamster PrP expression on the susceptibility to the hamster scrapie agent, we attempted to generate transgenic mice by using the hamster PrP open reading frame only, linked to appropriate regulatory 5' flanking sequences of brain specific genes. Although correct mRNA expression was achieved in some of the transgenic lines generated, problems were encountered with PrP protein production, indicating translational control of PrP. It was p

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19

Kirikyali, Narin. "Heterologous expression and characterisation of [beta]-xylosidases from fungal sources." Thesis, University of Nottingham, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.716838.

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Beta-xylosidases (E.C. 3.2.1.37) are a subclass of glycoside hydrolases (E C. 3.2.1.-) which hydrolyses the pi,4 glycosidic linkages of short chain xylo- oligosaccharides and xylobiose from the non-reducing termini releasing xylose monomers. They constitute a group of enzymes that have the biotechnological potential use in various industrial processes. As a consequence it has been a necessity for enzymes involved in hemicellulose hydrolysis, of industrial interest, to be individually identified and characterised in order to utilise them for practical purposes. In order to expand the range of a

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20

Wynant, Inneke. "Heterologous expression systems for metabolite production during early drug research." Doctoral thesis, Universite Libre de Bruxelles, 2010. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210070.

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La bio-transformation naturelle des médicaments peut produire des métabolites toxiques; l’identification de ces métabolites est essentielle dans la stratégie de choix de molécules thérapeutiques. En appliquant les technologies de fermentation en bioréacteur des cellules hétérologues (souches d’E. coli recombinantes exprimant une iso-enzyme de cytochrome P450 humain avec la réductase humain), la bioconversion du substrat (principe actif) en ses métabolites de dégradation, a été réalisée à grande échelle (&<br>Doctorat en Sciences<br>info:eu-repo/semantics/nonPublished

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21

O'Dwyer, Cliona Anne. "Expression and vaccine formulation of heterologous antigens using commensal Neisseria." Thesis, Imperial College London, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.401669.

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22

Carrier, David John. "The Arabidopsis Auxin Transporter Aux1 : Heterologous Expression, Characterisation and Purification." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.523129.

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23

Lloyd, John S. "Heterologous expression and site-directed mutagenesis of soluble methane monooxygenase." Thesis, University of Warwick, 1997. http://wrap.warwick.ac.uk/59600/.

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The purpose of this investigation was to study the heterologous expression of soluble methane monooxygenase (sMMO) genes from Methylococcus capsulatus (Bath) and Methylosinus trichosporium OB3b. Using the T7-RNA polymerase expression system, the entire sMMO operon and subclones (constructed using the polymerase chain reaction) were over-expressed in E. coli. Results obtained using the Me. capsulatus (Bath) sMMO operon confirmed previous reports (C. West, G. P. C. Salmond, H. Dalton and 1. C. Murrell (1992). J Gen. Microbial. 138, 1301-1307) that functional expression of protein B and the reduc

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24

Chitpinityol, Supannee. "Heterologous expression and site-directed mutagenesis of the enzyme chymosin." Thesis, University of Reading, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320101.

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25

Ekinci, Mehmet Sait. "Heterologous expression of genes in the anaerobic bacterium Streptococcus bovis." Thesis, University of Aberdeen, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387523.

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The main objective of this work was to investigate the expression of xylanase and cellulose genes from <I>Ruminococcus flavefaciens</I> when introduced into related Gram-positive bacteria including the rumen bacterium <I>Streptococcus bovis</I>. In addition the discovery, and isolation of the β(1,3-1,4)-glucanase gene of <I>S. bovis</I> in the course of this work may provide new possibilities to express foreign genes in <I>S. bovis</I> or in other Gram-positive bacteria. The main findings of this work are summarised below: 1. Genes encoding polysaccharidase activity from the strictly anaerobic

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26

Jordan, Bernadette. "Factors influencing heterologous gene expression in the yeast Saccharomyces cerevisiae." Thesis, University of Leicester, 1993. http://hdl.handle.net/2381/34432.

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The yeast Saccharomyces cerevisiae is widely employed as a host for the production of recombinant proteins. In the work presented here, expression of recombinant reporter proteins has been analysed in yeast, with the aim of determining factors that contribute to the expression performance of recombinant genes. Initially, expression of bacterial aminoglycoside phosphotransferase was analysed in yeast. This heterologous gene showed very low expression levels, resulting from a small decrease in mRNA abundance, plus a large decrease in translation efficiency. This appeared to be due to an unfavour

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27

Rahman, Md Mahbubar, Jay Shockey, and Aruna Kilaru. "Identification, and Heterologous Expression Analysis of Avocado DGAT1 and DGAT2." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etsu-works/4796.

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The neutral lipid triacylglycerol (TAG) is the main storage lipid in plants. When stored in seeds, TAG provides the carbon and energy source during germination. There is significant human nutritional demand for vegetable oil, but its use in production of renewable biomaterials and fuels has intensified the need to increase oil production. In plants, the final and committed step in TAG biosynthesis is catalyzed by diacylglycerol acyltransferases (DGAT) and/or a phospholipid: diacylglycerol acyltransferases (PDAT). Both DGAT and PDAT contribute to seed TAG biosynthesis in an independent or overl

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28

Shwed, Philip S. (Philip Steven) Carleton University Dissertation Biology. "A study of heterologous expression of Xenopus laevis histone H5." Ottawa, 1991.

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29

Bauly, James Matthew. "Studies on maize auxin-binding protein in two heterologous expression systems." Thesis, University of Bristol, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265478.

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30

Stevens, David Cole. "Developing Heterologous Expression Platforms for the Production of Polyketides from Microbial Hosts." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20220.

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Bacterial polyketides possess an enormous range of chemical diversity and biological function. Many polyketides such as tetracycline, epothilone, and rapamycin have been developed into key clinical pharmaceuticals in a broad range of therapeutic areas. Sequencing of bacterial genomes has shown that there are many more polyketide biosynthetic pathways than there are polyketides isolated from standard cultivation techniques. These genetically encoded polyketide natural products from cultivatable and uncultivatable bacteria represent one of the greatest remaining untapped reservoirs of new natur

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31

Gu, Lina. "Heterologous expression of manganese peroxidase from Phanerochaete chrysosporium in Pichia pastoris." Related Electronic Resource: Current Research at SU : database of SU dissertations, recent titles available full text, 2003. http://wwwlib.umi.com/cr/syr/main.

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32

Bergeld, Linnéa. "Trametes versicolor laccase: random mutagenesis and heterologous expression in Pichia pastoris." Thesis, Karlstad University, Division for Chemistry, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-1580.

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<p>Laccase is a blue multi-copper oxidase. It has a broad biotechnical potential which increases the interest to study the enzyme further. A laccase-encoding gene from the white-rot fungus Trametes versicolor (lcc2) was mutated using two different methods for random mutagenesis: error-prone PCR and a method based on an E.coli strain (ES1301 mutS) that introduces random mutations. For the error-prone PCR reaction, the vector pPICZB with the lcc2 gene inserted was used as template. The E. coli strain ES1301 mutS was transformed with the vector pBluescript SKII with the lcc2 gene as insert. The m

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33

Zhang, Feifei. "Heterologous expression of thiostrepton A and biosynthetic engineering of thiostrepton analogs." Diss., Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/54276.

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Thiopeptides are posttranslationally-processed macrocyclic peptide metabolites, characterized by extensive backbone and side chain modifications that include a six-membered nitrogenous ring, thioazol(in)e/oxazol(in)e rings, and dehydrated amino acid residues. Thiostrepton A, produced by Streptomyces laurentii ATCC 31255, is one of the more structurally complex thiopeptides, containing a second macrocycle bearing a quinaldic acid. Thiostrepton A and other thiopeptides are of great interest due to their potent activities against emerging antibiotic-resistant Gram-positive pathogens, in addition

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34

Venter, Philip Arno. "Heterologous expression of the helicoverpa armigera stunt virus in Saccharomyces cerevisiae." Thesis, Rhodes University, 2002. http://hdl.handle.net/10962/d1003811.

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Lepidopteran insects like Helicoverpa armigera, more commonly known as the cotton bollworm, are economically important pests of a wide variety of crops throughout the world. The Helicoverpa armigera stunt virus (HaSV), a tetravirus with a bipartite single-stranded positive-sense RNA genome, has great potential as a biological pesticide against H. armigera. The larger genomic strand of this virus (RNA1) encodes the viral replicase, while the other (RNA2) encodes the 71 kDa capsid protein precursor (p71). 240 copies of p71 assemble into a procapsid with the concomitant encapsidation of the viral

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35

Tiwari, Vijay. "Isolation and Heterologous Expression of Putative Tomato Fatty Acid Amide Hydrolase." Digital Commons @ East Tennessee State University, 2016. https://dc.etsu.edu/etd/3138.

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N-acylethanolamines (NAEs) are derived from a minor membrane lipid constituent N-acylphosphatidylethanolamine and are hydrolyzed by fatty acid amide hydrolases (FAAH) into free fatty acid (FFA) and ethanolamine in both plants and animals. In Arabidopsis, NAE plays an important physiological role in growth/development and response to stress. Although NAEs are reported in tomato, their metabolic pathway remains undiscovered. It is hypothesized that there is a functional FAAH in tomato that hydrolyzes NAEs. To this extent, a putative gene that likely encodes for putative SlFAAH1 protein was ident

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36

Ly, Mai Anh. "Evaluation of components for the heterologous expression of Mycobacterium chubuense NBB4 monooxygenases." Thesis, The University of Sydney, 2014. http://hdl.handle.net/2123/11638.

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Bacterial monooxygenases have important roles in the biogeochemical cycling of carbon and nitrogen, as well as potential applications in biocatalysis of pharmaceutically important epoxides, and bioremediation of chlorinated pollutants. However, use of monooxygenases for biotechnology requires understanding of microbial physiology and the catalytic properties (e.g. substrate range and kinetic parameters) of monooxygenases themselves. Heterologous expression of monooxygenases is one way to advance our fundamental knowledge of MO functions, and also to enable future applications. This project f

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37

黃佩珊 and Pui-shan Helen Wong. "Heterologous expression and immobilization of a haloalkane dehalogenase from Rhodococcus erythropolis Y2." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31226863.

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38

Wong, Pui-shan Helen. "Heterologous expression and immobilization of a haloalkane dehalogenase from Rhodococcus erythropolis Y2 /." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?

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39

Finol, Urdaneta Rocio Karin. "Investigation of the heterologous expression of the voltage activated potassium channel Kv1.7." Doctoral thesis, [S.l.] : [s.n.], 2004. http://webdoc.sub.gwdg.de/diss/2004/finol/finol.pdf.

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40

Setati, Mathabatha Evodia. "Heterologous production of family 5 fungal endo-1,4-B-mannanases in Saccharomyces cerevisiae." Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52875.

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Dissertation (PhD)--University of Stellenbosch, 2002.<br>ENGLISH ABSTRACT: Mannan polysaccharides occur in the hemicellulose fraction of plant cell walls, either as structural polymers or as reserve carbohydrates. They are found predominantly in the seeds of leguminous plants in the form of galactomannan, and in softwoods as galactoglucomannan. Endo-I,4-I3-mannanases hydrolyze mannan polysaccharides to oligosaccharides of various lengths. These enzymes are secreted as single catalytic modules or as part of multi-modular proteins by fungi, bacteria, plants and animals. For example, the l3

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41

Kallas, Åsa. "Heterologous expression, characterization and applications of carbohydrate active enzymes and binding modules." Doctoral thesis, KTH, Träbioteknik, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3950.

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Wood and wood products are of great economical and environmental importance, both in Sweden and globally. Biotechnology can be used both for achieving raw material of improved quality and for industrial processes such as biobleaching. Despite the enormous amount of carbon that is fixed as wood, the knowledge about the enzymes involved in the biosynthesis, re-organization and degradation of plant cell walls is relatively limited. In order to exploit enzymes more efficiently or to develop new biotechnological processes, it is crucial to gain a better understanding of the function and mechanism o

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42

Lamour, Jarryd. "Expression of stress-tolerance related genes in Saccharomyces cerevisiae producing heterologous cellobiohydrolase." University of the Western Cape, 2017. http://hdl.handle.net/11394/5885.

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Magister Scientiae - MSc (Biotechnology)<br>Cellulose is the most abundant naturally occurring renewable biopolymer on earth and a major structural component in plant cell walls, making it an ideal source of renewable energy. Consolidated bioprocessing (CBP) is a cost effective method of converting cellulose to liquid fuels such as ethanol. For CBP to be achieved an organism needs to be able hydrolyze cellulose and produce high yields of ethanol. The yeast Saccharomyces cerevisiae is an ideal CBP candidate, however wild type strains do not produce cellulases and these activities need to

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McKenzie, Belinda, and s9907915@student rmit edu au. "Heterologous expression of cellulase enzymes in transplastidic Nicotiana tabacum cv. Petit Havana." RMIT University. Applied Sciences, 2008. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080805.120923.

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Extensive research into enzyme-induced bio-conversion of lignocellulose to soluble sugars has been conducted and research continues in this area. Several approaches have been taken to attempt to alleviate the economic problems associated with utilisation of lignocellulose in fuel ethanol production. By expressing cellulase genes in planta, it is hoped that the cost of enzyme-mediated hydrolysis of cellulose to its soluble sugar monomers, will be reduced. Some accomplishments have been made in this area using nuclear genetic transformation (Abdeev et al., 2003; Abdeev et al., 2004; Austin

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Kallas, Åsa. "Heterologous expression, characterization and applications of carbohydrate active enzymes and binding modules /." Stockholm, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3950.

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45

Nijland, Reindert. "Heterologous expression and secretion of Clostridium perfringens [beta]-toxoid by Bacillus subtilis." [S.l. : Groningen : s.n. ; University Library Groningen] [Host], 2007. http://irs.ub.rug.nl/ppn/301153868.

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46

Matheson, Johanne. "Heterologous expression and functional properties of plaice and human cypia-family enzymes." Thesis, University of Stirling, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244518.

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47

Vickers, Louise. "Heterologous expression of Chlamydia trachomatis polymorphic membrane proteins for in vitro studies." Thesis, Sheffield Hallam University, 2013. http://shura.shu.ac.uk/20478/.

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Chlamydia trachomatis is the most common bacterial sexually transmitted disease worldwide. Not only does Chlamydia target urogenital tissue that can lead to host infertility, it is also responsible for a high occurence of blinding trachoma in the developing world. Complete sequencing analysis of the C. trachomatis genome revealed a unique family of Polymorphic membrane proteins (Pmps) that had previously remained undiscovered. Nine to twenty-one pmp genes were found across the varied Chlamdia species, encoding for large proteins all phylogetically related to one of six basic subtypes. C. tract

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48

Jovicevic, Dejana. "Developing a synthetic yeast for the expression of heterologous genes using SCRaMbLE." Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/60854.

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Synthetic genomics is a new and fast emerging multi-disciplinary field of research, representing the largest scale of work underway in synthetic biology. The Saccharomyces cerevisiae version 2 (Sc2.0) project is currently the leading example of synthetic genomics research, and is an attempt to perform the first redesign, synthesis and assembly of a complete synthetic genome for an eukaryotic organism. Major changes are being made to the redesigned DNA sequence of the new yeast genome and these include a variety of different deletions, sequence recodings at every gene and also insertions of DNA

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49

Kolar, Norbert Wilhelm. "An investigation of heterologous expression of human steroidogenic cytochromes P450 in yeasts." Thesis, Stellenbosch : Stellenbosch University, 2005. http://hdl.handle.net/10019.1/50271.

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Dissertation (PhD)--Stellenbosch University, 2005.<br>ENGLISH ABSTRACT: This study: 1. Compares various heterologous expression systems for high-level expression of cytochromes P450. Limitations of the existing cytochromes P450 expression systems are discussed and possibilities to improve the expression yields of human steroidogenic enzymes, are suggested. In addition the potential applications of human steroidogenic cytochromes P450 expressed in Pichia pastoris are illustrated. 2. Describes the cloning and extracellular expression of a recombinant full-length human cytochrome P450 17a

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50

De, Wet Barend Johannes Marthinus. "Characterisation, cloning and heterologous expression of the α-glucuronidase from Aureobasidium pullulans". Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/49876.

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Dissertation (PhD)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Xylanolytic accessory enzymes produced by the endo-p-l,4-xylanase overproducing, colour-variant strain of the euascomycetous fungus Aureobasidium pullulans, NRRL Y-2311-1, were studied. a- Glucuronidase activity was only induced during cultivation on carbon sources containing both xylose and glucuronic acid. An a-glucuronidase was partially purified from the supernatant of A. pullulans cultivated on birchwood glucuronoxylan. The enzyme had an apparent mobility on SDS-PAGE of 170 kDa, and after deglycosylation its

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