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Relevant bibliographies by topics / Heterologous proteins

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Academic literature on the topic 'Heterologous proteins'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 April 2022

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Journal articles on the topic "Heterologous proteins"

1

Park, Tae Jung, Kyung-Bok Lee, Seok Jae Lee, et al. "Micropatterns of Spores Displaying Heterologous Proteins." Journal of the American Chemical Society 126, no. 34 (2004): 10512–13. http://dx.doi.org/10.1021/ja047894y.

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Das, Rathin C., and Janice L. Shultz. "Secretion of Heterologous Proteins fromSaccharomyces Cerevisiae." Biotechnology Progress 3, no. 1 (1987): 43–48. http://dx.doi.org/10.1002/btpr.5420030108.

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GOODEY, A. "The production of heterologous plasma proteins." Trends in Biotechnology 11, no. 10 (1993): 430–33. http://dx.doi.org/10.1016/0167-7799(93)90007-v.

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Devchand, Medha, and David I. Gwynne. "Expression of heterologous proteins in Aspergillus." Journal of Biotechnology 17, no. 1 (1991): 3–9. http://dx.doi.org/10.1016/0168-1656(91)90022-n.

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Aon, Juan C., Richard J. Caimi, Alexander H. Taylor, et al. "Suppressing Posttranslational Gluconoylation of Heterologous Proteins by Metabolic Engineering of Escherichia coli." Applied and Environmental Microbiology 74, no. 4 (2007): 950–58. http://dx.doi.org/10.1128/aem.01790-07.

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ABSTRACT Minimization of chemical modifications during the production of proteins for pharmaceutical and medical applications is of fundamental and practical importance. The gluconoylation of heterologously expressed protein which is observed in Escherichia coli BL21(DE3) constitutes one such undesired posttranslational modification. We postulated that formation of gluconoylated/phosphogluconoylated products of heterologous proteins is caused by the accumulation of 6-phosphogluconolactone due to the absence of phosphogluconolactonase (PGL) in the pentose phosphate pathway. The results obtained

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Hoang, Huy-Dung, Jun-ichi Maruyama, and Katsuhiko Kitamoto. "Modulating Endoplasmic Reticulum-Golgi Cargo Receptors for Improving Secretion of Carrier-Fused Heterologous Proteins in the Filamentous Fungus Aspergillus oryzae." Applied and Environmental Microbiology 81, no. 2 (2014): 533–43. http://dx.doi.org/10.1128/aem.02133-14.

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ABSTRACTFilamentous fungi are excellent hosts for industrial protein production due to their superior secretory capacity; however, the yield of heterologous eukaryotic proteins is generally lower than that of fungal or endogenous proteins. Although activating protein folding machinery in the endoplasmic reticulum (ER) improves the yield, the importance of intracellular transport machinery for heterologous protein secretion is poorly understood. Here, usingAspergillus oryzaeas a model filamentous fungus, we studied the involvement of two putative lectin-like cargo receptors,A. oryzaeVip36 (AoVi

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Steffensen, Lotte, and Per Amstrup Pedersen. "Heterologous Expression of Membrane and Soluble Proteins Derepresses GCN4 mRNA Translation in the Yeast Saccharomyces cerevisiae." Eukaryotic Cell 5, no. 2 (2006): 248–61. http://dx.doi.org/10.1128/ec.5.2.248-261.2006.

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ABSTRACT This paper describes the first physiological response at the translational level towards heterologous protein production in Saccharomyces cerevisiae. In yeast, the phosphorylation of eukaryotic initiation factor 2α (eIF-2α) by Gcn2p protein kinase mediates derepression of GCN4 mRNA translation. Gcn4p is a transcription factor initially found to be required for transcriptional induction of genes responsible for amino acid or purine biosynthesis. Using various GCN4-lacZ fusions, knockout yeast strains, and anti-eIF-2α-P/anti-eIF-2α antibodies, we observed that heterologous expression of

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TSUCHIYA, Kozo. "Secretion of Heterologous Proteins from Filamentous Fungi." JOURNAL OF THE BREWING SOCIETY OF JAPAN 88, no. 7 (1993): 506–11. http://dx.doi.org/10.6013/jbrewsocjapan1988.88.506.

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Sleep, D., G. P. Belfield, D. J. Ballance, et al. "Saccharomyces Cerevisiae Strains that Overexpress Heterologous Proteins." Nature Biotechnology 9, no. 2 (1991): 183–87. http://dx.doi.org/10.1038/nbt0291-183.

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Chodosh, Lewis A., Albert S. Baldwin, Richard W. Carthew, and Phillip A. Sharp. "Human CCAAT-binding proteins have heterologous subunits." Cell 53, no. 1 (1988): 11–24. http://dx.doi.org/10.1016/0092-8674(88)90483-7.

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Dissertations / Theses on the topic "Heterologous proteins"

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Xu, Ping. "Sensing and analyzing unfolded protein response during heterologous protein production :." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 205 p, 2008. http://proquest.umi.com/pqdweb?did=1555621341&sid=2&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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Li, Zhiguo. "Structure, secretion, and proteolysis study of MBP-containing heterologous proteins in Pichia pastoris." Scholarly Commons, 2010. https://scholarlycommons.pacific.edu/uop_etds/2415.

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The E. coli maltose binding protein (MBP) has been utilized as a translational fusion partner to improve the expression of foreign proteins made in E. coli. When located N -terminal to its cargo protein, MBP increases the solubility of intracellular proteins and improves the export of secreted proteins in bacterial systems. We initially explored whether MBP would have the same effect in the methylotrophic yeast Pichia pastoris , a popular eukaryotic host for heterologous protein expression. When MBP was fused as an N -terminal partner to several C -terminal cargo proteins expressed in this yea

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Morgan, Cecilia A. "Treponema pallidum repeat protein K and heterologous protection against syphilis /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/9300.

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Vickers, Louise. "Heterologous expression of Chlamydia trachomatis polymorphic membrane proteins for in vitro studies." Thesis, Sheffield Hallam University, 2013. http://shura.shu.ac.uk/20478/.

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Chlamydia trachomatis is the most common bacterial sexually transmitted disease worldwide. Not only does Chlamydia target urogenital tissue that can lead to host infertility, it is also responsible for a high occurence of blinding trachoma in the developing world. Complete sequencing analysis of the C. trachomatis genome revealed a unique family of Polymorphic membrane proteins (Pmps) that had previously remained undiscovered. Nine to twenty-one pmp genes were found across the varied Chlamdia species, encoding for large proteins all phylogetically related to one of six basic subtypes. C. tract

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Anchim, Aleksandra. "Vaccination Potential Of Adenoviral Vectors Displaying Heterologous Epitopes In Their Capsid Proteins." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS070/document.

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Mes travaux de thèse ont pour but d’évaluer l’approche « épitope display » pour sa capacité d'induire les réponses cellulaires. Ad à capside modifiée par insertion de différents épitopes T issus de la ovalbumine ont étés produit. Après administration à des souris C57BL/6, j'ai mis en évidence l'induction de la réponse cellulaire dirigée contre les épitope insérés (grâce aux techniques ELISPOT, tétramères, ou bien en quantifiant la production d’IFNg par les splénocytes restimulés in vitro). J’ai démontré que ces réponses sont limités chez des souris préalablement immunisées avec Ad. Des manipul

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Gorgens, Johann Ferdinand. "Quantitative yeast physiology and nitrogen metabolism during heterologous protein production." Thesis, Stellenbosch : University of Stellenbosch, 2003. http://hdl.handle.net/10019.1/16051.

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Dissertation (PhD)--University of Stellenbosch, 2003.<br>ENGLISH ABSTRACT: QUANTITATIVE YEAST PHYSIOLOGY AND NITROGEN METABOLISM DURING HETEROLOGOUS PROTEIN PRODUCTION By Johann F. Görgens The physiology and nitrogen metabolism of the yeast, Saccharomyces cerevisiae, during heterologous xylanase production in a defined medium was quantified by the comparison of isogenic yeast strains, whereby several potential limitations in the production of the heterologous xylanase could be identified. The presence of global sensing and regulatory mechanisms, by which the yeast is able to actively re

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Kriel, Johan Hendrik. "Development of synthetic signal sequences for heterologous protein secretion from Saccharomyces cerevisiae." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53364.

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Thesis (MSc)--Stellenbosch University, 2003.<br>ENGLISH ABSTRACT: Protein secretion and intracellular transport are highly regulated processes and involve the interplay of a multitude of proteins. A unique collection of thermosensitive secretory mutants allowed scientists to demonstrate that the secretory pathway of the yeast Saccharomyces cerevisiae is very similar to that of the higher eukaryotes. All proteins commence their journey in the endoplasmic reticulum, where they undergo amino-linked core glycosyl modification. After passage through the Golgi apparatus, where the remodelling

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Koscky, Paier Carlos Roberto 1983. "Padronização da expressão heterologa e de modelo de ensaio de atividade para a proteina quinase humana S6K." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314787.

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Orientador: Nilson Ivo Tonin Zanchin<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-08-14T12:40:52Z (GMT). No. of bitstreams: 1 KosckyPaier_CarlosRoberto_M.pdf: 3760581 bytes, checksum: 99331529324819b59a4360d60efd9b9a (MD5) Previous issue date: 2009<br>Resumo: A quinase de 70 kDa da proteína ribossomal S6, isoforma 1 (S6K1), é uma fosfoproteína implicada na regulação de genes relacionados ao controle da tradução em mamíferos e possui uma forma nuclear (a1) e uma citoplasmática (a2). A fosforilação do seu principal alv

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Brockmeier, Ulf. "New strategies to optimize the secretion capacity for heterologous proteins in Bacillus subtilis." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=980650771.

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Matthäus, Falk. "Heterologous expression and purification of wheat storage proteins in the yeast Saccharomyces cerevisiae." [S.l.] : [s.n.], 2006. http://opus.kobv.de/tuberlin/volltexte/2007/1564.

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Books on the topic "Heterologous proteins"

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Mus-Veteau, Isabelle, ed. Heterologous Expression of Membrane Proteins. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3637-3.

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Mus-Veteau, Isabelle, ed. Heterologous Expression of Membrane Proteins. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-344-2.

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Heterologous expression of membrane proteins: Methods and protocols. Humana Press, 2010.

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Meleady, Paula, ed. Heterologous Protein Production in CHO Cells. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6972-2.

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Mannonen, Leena. Barley cell culture as a producer of heterologous protein. Technical Research Centre of Finland, 1993.

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Anwar, Rashida. The control of heterologous protein production in recombinant Chinese hamster cells. University of Manchester, 1994.

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Verrall, B. Expresssion of the TATA box binding protein of plasmodium falciparum in heterologous systems. UMIST, 1997.

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Heterologous Expression of Membrane Proteins: Methods and Protocols. Humana, 2018.

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Heterologous Protein Production in CHO Cells: Methods and Protocols. Humana, 2018.

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Book chapters on the topic "Heterologous proteins"

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Mannonen, Leena, Kristian Aspegren, Anneli Ritala, Hanna Simola, and Teemu H. Teeri. "Barley as a Producer of Heterologous Protein." In Recombinant Proteins from Plants. Humana Press, 1998. http://dx.doi.org/10.1007/978-1-60327-260-5_2.

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Sauer, Michael, Paola Branduardi, Hannes Rußmayer, Hans Marx, Danilo Porro, and Diethard Mattanovich. "Production of Metabolites and Heterologous Proteins." In Molecular Mechanisms in Yeast Carbon Metabolism. Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-662-45782-5_11.

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Napier, Johnathan A., Gaelle Richard, and Peter R. Shewry. "Trafficking and Stability of Heterologous Proteins in Transgenic Plants." In Recombinant Proteins from Plants. Humana Press, 1998. http://dx.doi.org/10.1007/978-1-60327-260-5_15.

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Sakuraba, Haruhiko, and Toshihisa Ohshima. "Heterologous Production of Thermostable Proteins and Enzymes." In Thermophilic Microbes in Environmental and Industrial Biotechnology. Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-5899-5_15.

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Manfredi, Francesco, Paola Di Bonito, Claudia Arenaccio, Simona Anticoli, and Maurizio Federico. "Incorporation of Heterologous Proteins in Engineered Exosomes." In Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3753-0_18.

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Li, Huijuan, Robert G. Miele, Teresa I. Mitchell, and Tillman U. Gerngross. "N-Linked Glycan Characterization of Heterologous Proteins." In Methods in Molecular Biology. Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-456-8_10.

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Bruyns, Anne-Marie, Myriam De Neve, Geert De Jaeger, Chris De Wilde, Pierre Rouzé, and Ann Depicker. "Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA." In Recombinant Proteins from Plants. Humana Press, 1998. http://dx.doi.org/10.1007/978-1-60327-260-5_18.

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Mus-Veteau, Isabelle. "Heterologous Expression of Membrane Proteins for Structural Analysis." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-344-2_1.

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MacKenzie, D. A., D. J. Jeenes, and D. B. Archer. "Filamentous Fungi as Expression Systems for Heterologous Proteins." In Genetics and Biotechnology. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-662-07426-8_15.

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Becker-Pauly, Christoph, and Walter Stöcker. "Insect Cells for Heterologous Production of Recombinant Proteins." In Insect Biotechnology. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9641-8_10.

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Conference papers on the topic "Heterologous proteins"

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Wang, Jianye, Kaixuan Zhu, Gang Zhao, and Dayong Gao. "Effect of Temperature and Cryoprotectant Solutes on Water Permeability of SF21 Cell Membrane." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14056.

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Insect cell as a host of viruses is extensively used for producing heterologous recombinant proteins. The eukaryotic proteins expressed by the insect cell is posttranslationally modified and harvested in a short period of time. The insect cell expression has been applied to both basic research and commercial applications. A large scale of proteins produced by the insect cell expression system are settled for researching the structure and function of the eukaryotic proteins, and the expression system integrated with routine biochemical techniques plays a significant role in diagnostic procedure

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Rogozin, E. "Biotechnology for production of recombinant hybrid proteins from plants and microbes with antifungal activity." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.206.

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The principle of obtaining recombinant antimicrobial polypeptides from plant and microbial origins as a part of chimeric proteins with thioredoxin by heterologous expression in a prokaryotic system is presented. The results obtained in terms of their antifungal activity in relation to plant pathogenic micromycetes allow us to consider these compounds as prototypes of some active substances of environmentally friendly biofungicides, as well as possible components of hybrid plant protection products against fungal diseases.

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Mirzahoseini, Hasan, Samaneh Mafakheri, Somayeh Enayati, and Nahid Mortazavi. "Heterologous proteins expression in Escherichia coli: investigation of the effect of codon usage and expression host optimization." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0121.

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Pereira, Victor, Marcos Schwarz, and Leila Lima. "Use of a theophylline responsive riboswitch for translational control applied to the expression of secreted heterologous proteins in Mycobacterium smegmatis." In International Symposium on Immunobiological. Instituto de Tecnologia em Imunobiológicos, 2021. http://dx.doi.org/10.35259/isi.2021_46598.

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Karges, H. E., G. Zettlemeiβl, H. Naumann, U. Eberhard, and M. Bröker. "PURIFICATION AND CHARACTERIZATION OF GENTECHNOLOGICALLY PREPARED ANTITHROMBIN III." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643684.

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Isolation and purification of antithrombin III (AT III) by affinity chromatography on immobilized heparin is a standard method for the large scale preparation of this protein from human or animal plasma. Hence, after AT III became available by gentechnological methods, we tried to adapt this procedure for the isolation of AT III from supernatants of mammalian- and yeast-cells. Indeed, it was possible to use this method also for the isolation of the recombinant gene products. Since, however, the cell growth media contain heterologous protein or peptide mixtures like fetal calf serum, the method

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LeDuc, Philip R., and Michael J. Betenbaugh. "Implementation of a Pharmocokinetic Approach to a Baculovirus System for Analytic Solutions to Virus and Cell Interactions." In ASME 1997 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1997. http://dx.doi.org/10.1115/imece1997-0282.

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Abstract The baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV), expression system can be employed for a variety of different cellular applications. In recent years, this baculovirus system has been manipulated to serve as a recombinant system for the expression of heterologous proteins and as a possible retrovirus for gene therapy. A quantitative understanding of the cellular mechanics of virus trafficking would be useful in developing viral expression systems, understanding gene therapy and maximizing recombinant protein production. An analytic solution is prese

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Brodsky, G. L., та S. P. Bajaj. "DETERMINATION OF NUMBER OF γ-CARBOXYGLUTAMIC ACID (GLA) RESIDUES INVOLVED IN FORMING THE TWO HIGH AFFINITY METAL BINDING SITES IN PROTHROMBIN AND FACTOR X". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643934.

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Prothrombin and factor X possess two high affinity and several low affinity lanthanide ion binding sites. In both proteins, the association constant of the high affinity sites is at least 50-fold greater than that of the low affinity sites. Moreover, metal bound to these high affinity sites is extremely difficult to displace. It has been proposed that one of the two high affinity sites in factor X involves Gla residues while the other involves β-hydroxyaspartic acid and no Gla residues. It is also known that ^H can be specifically incorporated into Gla residues at an acidic pH. We have determi

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Ruan, X. Xi and C., D. Cheng, and H. Wan. "THE APPLICATIONS OF THE IMMUNOADSORBENT CHROMATOGRAPHY USING MO NOCLONAL ANTIBODY TO VON WILLEBRAND FACTOR." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644898.

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We have produced a group of monoclonal antibodiesto human von Willebrand factor (vWF) which specifically bound to human vWF but did not react with coagulation factors and other adhesive proteins, i.e. fibrinogen, fibronectin and thrombospondin. One of these antibodies, namely SZ _ 29, which bound to all multimers of vWF, was chosen for the immunoadsorbent chromatography. 15mg of purified SZ _ 29 IgG were coupled to 1.5g of BrCN activated Sepharose 4B gel. Theimmobilized IgG were packed into a glass column 1.0cm in internal diameter and 10cm in length, and equilibrated with 0.05M Tris buffer pH

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Sadler, J. Evan. "THE MOLECULAR BIOLOGY OF VON WILLEBRAND FACTOR." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643930.

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Human von Willebrand factor (vWF) is a plasma glycoprotein that is synthesized by endothelial cells and megakaryocytes, and perhaps by syncytiotrophoblast of placenta. The biosynthesis of vWF is very complex, involving proteolytic processing, glycosyla-tion, disulfide bond formation, and sulfation. Mature vWF consists of a single subunit of ∼ 250,000 daltons that is assembled into multimer ranging from dimers to species of over 10 million daltons. vWF performs its essential hemostatic function through several binding interactions, forming a bridge between specific receptors on the platelet sur

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Vu, S. K., A. A. Belloti, C. J. Gabriel, et al. "Modeling ribosome dynamics to optimize heterologous protein production in escherichia coli." In 2014 IEEE Global Conference on Signal and Information Processing (GlobalSIP). IEEE, 2014. http://dx.doi.org/10.1109/globalsip.2014.7032363.

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Reports on the topic "Heterologous proteins"

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Flowers, Ann M. Secretion of Heterologous Proteins from Escherichia coli. Defense Technical Information Center, 2000. http://dx.doi.org/10.21236/ada391190.

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Anderson, Olin, and Gad Galili. Development of Assay Systems for Bioengineering Proteins that Affect Dough Quality and Wheat Utilization. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568781.bard.

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The quality and utilization of wheat is largely dependent upon the exact physical/chemical properties of the doughs made from flour/water mixtures. Among the wheat seed components most correlated with dough visoelastic parameters are the high-molecular-weight (HMW) glutenin subunits whose disulfide cross-linked macropolymer is critical for dough functionality. We have used the tools of molecular biology, wheat transformation, heterologous expression of HMW-glutenin subunits in bacteria, and dough micro-mixing experiments to examine some of the molecular basis of HMW-glutenin functionality. In

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Anderson, Olin, Gad Galili, and Ann Blechl. Heterologous Expression of Wheat High Molecular Weight Glutenin Subunit Genes: Analysis and Modification of Protein Sequences Affecting Dough Quality. United States Department of Agriculture, 1993. http://dx.doi.org/10.32747/1993.7603826.bard.

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Perk, Simon, Egbert Mundt, Alexander Panshin, et al. Characterization and Control Strategies of Low Pathogenic Avian Influenza Virus H9N2. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7697117.bard.

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The avian influenza virus, subtype H9N2 subtype, defined as having a low pathogenicity, causes extensive economical losses in commercial flocks, probably due to management and synergism with other pathogens. AIV H9N2 was first identified in Israel in the year 2000, and since then it became endemic and widespread in Israel. Control by vaccination of commercial flocks with an inactivated vaccine has been introduced since 2007. In face of the continuous H9N2 outbreaks, and the application of the vaccination policy, we aimed in the present study to provide a method of differentiating naturally inf

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Jander, Georg, and Daniel Chamovitz. Investigation of growth regulation by maize benzoxazinoid breakdown products. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7600031.bard.

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Introduction Previous research had suggested that benzoxazinoids, a class of defensive metabolites found in maize, wheat, rye, and wild barley, are not only direct insect deterrents, but also influence other areas of plant metabolism. In particular, the benzoxazinoid 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxa- zin-3(4H)- one (DIMBOA) was implicated in: (i) altering plant growth by interfering with auxin signaling, and (ii) leading to the induction of gene expression changes and secondary plant defense responses. The overall goal of this proposal was to identify mechanisms by which benzoxazinoids i

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Bar-Joseph, Moshe, William O. Dawson, and Munir Mawassi. Role of Defective RNAs in Citrus Tristeza Virus Diseases. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575279.bard.

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This program focused on citrus tristeza virus (CTV), the largest and one of the most complex RNA-plant-viruses. The economic importance of this virus to the US and Israeli citrus industries, its uniqueness among RNA viruses and the possibility to tame the virus and eventually turn it into a useful tool for the protection and genetic improvement of citrus trees justify these continued efforts. Although the overall goal of this project was to study the role(s) of CTV associated defective (d)-RNAs in CTV-induced diseases, considerable research efforts had to be devoted to the engineering of the h

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Wisniewski, Michael E., Samir Droby, John L. Norelli, Noa Sela, and Elena Levin. Genetic and transcriptomic analysis of postharvest decay resistance in Malus sieversii and the characterization of pathogenicity effectors in Penicillium expansum. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7600013.bard.

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Blue mold of apple caused by Penicilliumexpansumis a major postharvest disease. Selection for postharvest disease resistance in breeding programs has been ignored in favor of fruit quality traits such as size, color, taste, etc. The identification of postharvest disease resistance as a heritable trait would represent a significant accomplishment and has not been attempted in apple. Furthermore, insight into the biology of the pathogenicity of P. expansumin apple could provide new approaches to postharvest decay management. Hypothesis: Postharvest resistance of apple to P. expansumcan be mapped

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Perl, Avichai, Bruce I. Reisch, and Ofra Lotan. Transgenic Endochitinase Producing Grapevine for the Improvement of Resistance to Powdery Mildew (Uncinula necator). United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568766.bard.

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The original objectives are listed below: 1. Design vectors for constitutive expression of endochitinase from Trichoderma harzianum strain P1. Design vectors with signal peptides to target gene expression. 2. Extend transformation/regeneration technology to other cultivars of importance in the U.S. and Israel. 3. Transform cultivars with the endochitinase constructs developed as part of objective 1. A. Characterize foliar powdery mildew resistance in transgenic plants. Background of the topic Conventional breeding of grapevines is a slow and imprecise process. The long generation cycle, large

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Gelb, Jr., Jack, Yoram Weisman, Brian Ladman, and Rosie Meir. Identification of Avian Infectious Brochitis Virus Variant Serotypes and Subtypes by PCR Product Cycle Sequencing for the Rational Selection of Effective Vaccines. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586470.bard.

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Objectives 1. Determine the serotypic identities of 40 recent IBV isolates from commercial chickens raised in the USA and Israel. 2. Sequence all IBV field isolates using PCR product cycle sequencing and analyze their S 1 sequence to detennine their homology to other strains in the Genbank and EMBL databases. 3. Select vaccinal strains with the highest S 1 sequence homology to the field isolates and perform challenge of immunity studies in chickens in laboratory trials to detennine level of protection afforded by the vaccines. Background Infectious bronchitis (IB) is a common, economically imp

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Lers, Amnon, and Pamela J. Green. LX Senescence-Induced Ribonuclease in Tomato: Function and Regulation. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586455.bard.

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Natural leaf senescence, which occurs even when growth conditions are near optimal, has a negative influence on yield. Postharvest induced senescence contributes to the losses of quality in flowers, foliage, and vegetables. Strategies designed to control the senescence process in crop plants could therefore have great applied significance. However, the successful design of such strategies requires a better insight into the senescence machinery and control in higher plants. A main feature of senescence is the hydrolysis of macromolecules by hydrolases of various types such as ribonucleases (RNa

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