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Dissertations / Theses on the topic 'Heterologous proteins'

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Published: 4 June 2021
Last updated: 25 April 2022

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1

Xu, Ping. "Sensing and analyzing unfolded protein response during heterologous protein production :." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 205 p, 2008. http://proquest.umi.com/pqdweb?did=1555621341&sid=2&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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Li, Zhiguo. "Structure, secretion, and proteolysis study of MBP-containing heterologous proteins in Pichia pastoris." Scholarly Commons, 2010. https://scholarlycommons.pacific.edu/uop_etds/2415.

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The E. coli maltose binding protein (MBP) has been utilized as a translational fusion partner to improve the expression of foreign proteins made in E. coli. When located N -terminal to its cargo protein, MBP increases the solubility of intracellular proteins and improves the export of secreted proteins in bacterial systems. We initially explored whether MBP would have the same effect in the methylotrophic yeast Pichia pastoris , a popular eukaryotic host for heterologous protein expression. When MBP was fused as an N -terminal partner to several C -terminal cargo proteins expressed in this yea
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Morgan, Cecilia A. "Treponema pallidum repeat protein K and heterologous protection against syphilis /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/9300.

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Vickers, Louise. "Heterologous expression of Chlamydia trachomatis polymorphic membrane proteins for in vitro studies." Thesis, Sheffield Hallam University, 2013. http://shura.shu.ac.uk/20478/.

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Chlamydia trachomatis is the most common bacterial sexually transmitted disease worldwide. Not only does Chlamydia target urogenital tissue that can lead to host infertility, it is also responsible for a high occurence of blinding trachoma in the developing world. Complete sequencing analysis of the C. trachomatis genome revealed a unique family of Polymorphic membrane proteins (Pmps) that had previously remained undiscovered. Nine to twenty-one pmp genes were found across the varied Chlamdia species, encoding for large proteins all phylogetically related to one of six basic subtypes. C. tract
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Anchim, Aleksandra. "Vaccination Potential Of Adenoviral Vectors Displaying Heterologous Epitopes In Their Capsid Proteins." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS070/document.

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Mes travaux de thèse ont pour but d’évaluer l’approche « épitope display » pour sa capacité d'induire les réponses cellulaires. Ad à capside modifiée par insertion de différents épitopes T issus de la ovalbumine ont étés produit. Après administration à des souris C57BL/6, j'ai mis en évidence l'induction de la réponse cellulaire dirigée contre les épitope insérés (grâce aux techniques ELISPOT, tétramères, ou bien en quantifiant la production d’IFNg par les splénocytes restimulés in vitro). J’ai démontré que ces réponses sont limités chez des souris préalablement immunisées avec Ad. Des manipul
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Gorgens, Johann Ferdinand. "Quantitative yeast physiology and nitrogen metabolism during heterologous protein production." Thesis, Stellenbosch : University of Stellenbosch, 2003. http://hdl.handle.net/10019.1/16051.

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Dissertation (PhD)--University of Stellenbosch, 2003.<br>ENGLISH ABSTRACT: QUANTITATIVE YEAST PHYSIOLOGY AND NITROGEN METABOLISM DURING HETEROLOGOUS PROTEIN PRODUCTION By Johann F. Görgens The physiology and nitrogen metabolism of the yeast, Saccharomyces cerevisiae, during heterologous xylanase production in a defined medium was quantified by the comparison of isogenic yeast strains, whereby several potential limitations in the production of the heterologous xylanase could be identified. The presence of global sensing and regulatory mechanisms, by which the yeast is able to actively re
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Kriel, Johan Hendrik. "Development of synthetic signal sequences for heterologous protein secretion from Saccharomyces cerevisiae." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53364.

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Thesis (MSc)--Stellenbosch University, 2003.<br>ENGLISH ABSTRACT: Protein secretion and intracellular transport are highly regulated processes and involve the interplay of a multitude of proteins. A unique collection of thermosensitive secretory mutants allowed scientists to demonstrate that the secretory pathway of the yeast Saccharomyces cerevisiae is very similar to that of the higher eukaryotes. All proteins commence their journey in the endoplasmic reticulum, where they undergo amino-linked core glycosyl modification. After passage through the Golgi apparatus, where the remodelling
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Koscky, Paier Carlos Roberto 1983. "Padronização da expressão heterologa e de modelo de ensaio de atividade para a proteina quinase humana S6K." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314787.

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Orientador: Nilson Ivo Tonin Zanchin<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-08-14T12:40:52Z (GMT). No. of bitstreams: 1 KosckyPaier_CarlosRoberto_M.pdf: 3760581 bytes, checksum: 99331529324819b59a4360d60efd9b9a (MD5) Previous issue date: 2009<br>Resumo: A quinase de 70 kDa da proteína ribossomal S6, isoforma 1 (S6K1), é uma fosfoproteína implicada na regulação de genes relacionados ao controle da tradução em mamíferos e possui uma forma nuclear (a1) e uma citoplasmática (a2). A fosforilação do seu principal alv
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Brockmeier, Ulf. "New strategies to optimize the secretion capacity for heterologous proteins in Bacillus subtilis." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=980650771.

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Matthäus, Falk. "Heterologous expression and purification of wheat storage proteins in the yeast Saccharomyces cerevisiae." [S.l.] : [s.n.], 2006. http://opus.kobv.de/tuberlin/volltexte/2007/1564.

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11

Beriotto, Irene. "Optimising the autotransporter system for secretion and display of heterologous proteins on GMMA." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7652/.

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The Pet Autotransporter protein was engineered and recently proposed as recombinant protein production (RPP) system. This system allows targeting the protein of interest in the culture supernatant fraction. The reduction of diversity and quantity of process impurities and size and number of downstream steps required, increase the overall process robustness and speed-up the process development time for RPP. In the context of this study the platform was investigated for the production of a “difficult” E.coli protein with commercial relevance, C1275. Pet autotransporter was suitable for the produ
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Haukaas, Tonje Husby. "Development of Expression Systems and Cultivation Conditions for Production of Heterologous Proteins in Pseudomonas." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for bioteknologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-12732.

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This study has been part of the project “Development of versatile bacterial expression systems for use in recombinant protein production, metabolic engineering, and systems biology”, a collaboration between NTNU, SINTEF (Department for Biotechnology) and SU (Saarland University, Germany). The major goal of both the project and this study was to develop and apply advanced biological tools for control of gene expression. Recombinant DNA, created by artificially producing genetic sequences, can be transferred to microorganisms and change their properties. One new possible property is the producti
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Vukov, Nataša. "Functional analysis of heterologous holin proteins in a lDSthf [lambda-Delta-Sthf] genetic background." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=965254534.

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Gardner, Kate B. "Functional assessment of transport proteins from Plasmodium falciparum via heterologous expression and evolutionary analysis." Thesis, St George's, University of London, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.546792.

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15

Smyth, Lesley Ann. "Specific immune response and tolerance in mice transgenic for heterologous proteins secreted in milk." Thesis, University of Edinburgh, 1995. http://hdl.handle.net/1842/11418.

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Systemic tolerance to BLG was studied in a) male and female mice heterozygous for the BLG transgene derived from crossing homozygous BLG transgenic males with wild-type females, b) BLG-transgenic and non-transgenic offspring derived from mating male and female heterozygous for the BLG transgene with wild type partners and c) BLG-transgenic and non-transgenic mice derived from back crossing onto a CBA/Ca MHC background. Using either a BLG-specific ELISA (for antibody responses) or footpad thickening assay (for T cell responses) the immune response to the BLG antigen was assessed. Hypo-responsiv
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Greenfield, Julia Josephine Anna. "Heterologous expression of modified wheat and barley storage proteins for structural and biophysical studies." Thesis, University of Bristol, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336868.

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17

Roth, Robyn Lindsay. "The evaluation of heterologous eukaryotic expression systems for the production of biocatalytic enzymes." Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/21733.

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Thesis (PhD)--Stellenbosch University, 2008.<br>ENGLISH ABSTRACT: Heterologous gene expression is of considerable interest for the production of proteins of therapeutic and industrial importance. As the nature of recombinant proteins has become more complex and as transformation systems have been established in more species, so the variety of hosts available for expression has increased. Every system available has both advantages and disadvantages. The research presented here highlights the advantages of selecting the most appropriate expression system for different recombinant proteins.
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18

Parker, Breck Olland. "Repair of DNA Containing Small Heterologous Sequences by Escherichia Coli: a Dissertation." eScholarship@UMMS, 1991. https://escholarship.umassmed.edu/gsbs_diss/123.

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The Dam-dependent mismatch repair system of Escherichia coli is part of a large network of DNA surveillance and error avoidance systems that identify and repair DNA damage. In this thesis, I have investigated the Dam-dependent mismatch repair system of E. coliand its role in the recognition and repair of DNA substrate molecules containing small insertion/deletion heterologies. This investigation was divided into two parts: the first part utilized genetic techniques to evaluate the specificity of repair and the second part utilized biochemical approaches into the recognition of insertion/deleti
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Ali, Zahid. "Developing strategies for homologous, heterologous plant expression system for physiological investigations of respective target proteins." kostenfrei, 2007. http://d-nb.info/987031201/34.

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Dastoor, Farahad P. "Evaluation of pullulanase for secretion of enzymatically active heterologous cellulases as chimeric proteins from Klebsiella oxytoca." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0028/NQ38876.pdf.

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21

Wagner, Nazgül Verfasser], Rainer [Akademischer Betreuer] [Fischer, and Ralph [Akademischer Betreuer] Panstruga. "Host plant modifications to improve production of challenging heterologous proteins / Nazgül Wagner ; Rainer Fischer, Ralph Panstruga." Aachen : Universitätsbibliothek der RWTH Aachen, 2018. http://d-nb.info/1194184065/34.

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Jung, Cindy. "Quantitative analysis of lentivirus incorporation of heterologous viral and non-viral proteins for lung gene therapy." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/26648.

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Thesis (Ph.D)--Biomedical Engineering, Georgia Institute of Technology, 2008.<br>Committee Chair: Joseph M. Le Doux; Committee Member: Andrés J. Garcia; Committee Member: Cheng Zhu; Committee Member: Nael McCarty; Committee Member: Richard Compans. Part of the SMARTech Electronic Thesis and Dissertation Collection.
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Wagner, Nazgül [Verfasser], Rainer [Akademischer Betreuer] Fischer, and Ralph [Akademischer Betreuer] Panstruga. "Host plant modifications to improve production of challenging heterologous proteins / Nazgül Wagner ; Rainer Fischer, Ralph Panstruga." Aachen : Universitätsbibliothek der RWTH Aachen, 2018. http://d-nb.info/1194184065/34.

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Lee, Dong-Hee. "Study of zein protein body formation in a heterologous system (Xenopus laevis oocyte)." Diss., Virginia Tech, 1992. http://hdl.handle.net/10919/39717.

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Most seed storage proteins accumulate in protein bodies which are derived from the vacuole. Zeins, the major corn storage proteins, however, are retained in the endoplasmic reticulum (ER) and their protein bodies are derived from the ER. There are circumstantial and preliminary data indicating that 27K zein, the proline-rich zein, may span the ER membrane. This potential transmembrane feature is considered very significant to understand the mechanism for zeins' ER retention. The transmembrane feature may retain the 27K zein in the ER where it could serve as an anchor for other classes of zein
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Charewa, Wellington. "Increasing ethanol tolerance through the heterologous expression of the geobacillus thermoglucosiadius heat-shock proteins (GroEL/GroES) in an escherichia coli host." University of the Western Cape, 2012. http://hdl.handle.net/11394/5325.

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>Magister Scientiae - MSc<br>Due to economic and environmental concerns associated with use of fossil fuels, humanity is seeking alternative fuels. Ethanol is one of the alternative fuels produced commercially. Current ethanol production technologies using first generation ethanol processes is criticised for depleting the food supply and escalating food prices. Biomass is a target feedstock for use in bioethanol production and would resolve the criticism associated with the current bioethanol industry. Bacterial strains such as Geobacillus thermoglucosidasius NCIMB 11955 can be used to produce
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Trexler, Melody May. "A cyclical semi-continuous process for production of heterologous proteins in metabolically regulated plant cell suspension cultures /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2003. http://uclibs.org/PID/11984.

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Rachidi, Makgangtsake Dominic. "Heterologous expression of alcelaphine herpesvirus 1 structural proteins and their use in the development of an ELISA." Diss., University of Pretoria, 2013. http://hdl.handle.net/2263/40709.

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Malignant catarrhal fever (MCF), a disease that is usually fatal in cattle, is caused by two distinct but related bovine herpesviruses which are members of the genus Macavirus. The wildebeest-associated alcelaphine herpesvirus-1 (AlHV-1) occurs mainly in East and southern Africa, whereas the sheep-associated ovine herpesvirus-1 (OvHV-2) has an almost worldwide distribution. The natural hosts or carriers of these two viruses are subclinically infected. The 130 kilobase pair (kbp) AlHV-1 double stranded DNA genome consists of 18 open reading frames (ORFs) coding for structural proteins and
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Lamy, Anaïs. "Lipid Flippases from Plasmodium Parasites : from Heterologous Production towards Functional Characterization." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS447/document.

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Le paludisme est une maladie dévastatrice causée par un parasite du genre Plasmodium. Du fait de la propagation de souches résistantes aux actuels antipaludéens, il est nécessaire de comprendre les fonctions physiologiques essentielles du parasite afin de trouver de nouvelles cibles thérapeutiques. Les transporteurs membranaires sont une classe importante de cibles chez l'homme du fait de leur rôle physiologique essentiel pour la cellule. Cependant, chez les parasite du genre Plasmodium, seulement quelques transporteurs ont été biochimiquement caractérisés. Des études récentes de délétion de g
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Thomas, Graham. "The host-pathogen interface : characterising putative secreted proteins of the honeybee pathogen Nosema ceranae (Microsporidia )." Thesis, University of Exeter, 2015. http://hdl.handle.net/10871/21445.

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Microsporidia are obligate intracellular eukaryotic parasites related to fungi, possessing greatly reduced genomic and cellular components. The microsporidian Nosema ceranae threatens honeybee (Apis mellifera) populations. Nosemosis has a complex epidemiology affected by host, pathogen and environmental factors. Although a draft of the N. ceranae genome has been published, the molecular basis underpinning pathogenicity is not known. The lack of established culturing techniques and a tractable genetic system necessitates use of model systems for both host and parasite such as Saccharomyces cere
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Bélanger, Louise. "Development of Methylobacterium extorquens as a recombinant protein production system and the expression of the heterologous cry1Aa gene from Bacillus thuringiensis." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=78244.

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Methylobacterium extorquens ATCC55366 is an interesting candidate for large-scale production of recombinant proteins. Development and optimization of this recombinant expression system were done using the green fluorescent protein (GFP) gene cloned into expression vectors (pRK310 and pCM110) as model systems. Selection of efficient GFP-expressing clones, long-term production stability without selection in flasks, effects of selection, oxygen and methanol supplies, were studied during fed-batch fermentations in a 20-l bioreactor. Sequential batch-culture cultivations in shake flasks show
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Bezbaruah, Supriya. "Heterologous expression of the mammalian microtubule associated proteins (MAPs), TAU, MAP2C and MAP4 in the fission yeast schizosaccharomyces pombe." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322302.

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Lawuyi, Olabisi. "A novel approach to oral therapy : preparation and in vitro analysis of microencapsulated engineered Lactococcus lactis cells secreting heterologous proteins." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98985.

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Lactococcus lactis are intensely being studied for the oral delivery of vaccines and other therapeutics to the mucosa of the gastrointesintal tract (GIT). In this thesis, we explore a new development by applying microencapsulation technology. A comparative study of the viability and protein secretion of free and alginate-poly-l-lysine-alginate (APA) encapsulated engineered L. lactis has demonstrated the effectiveness of this new system. The viability of microcapsules in in vitro culture conditions and simulated gastrointestinal (SGI) environments was also investigated. The latter condition exh
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Macedo, Vranas Marta [Verfasser], and Thorsten [Akademischer Betreuer] Friedrich. "Heterologous production, purification and characterization of respiratory proteins from Aquifex aeolicus in Escherichia coli strains = Heterologe Produktion, Reinigung und Charakterisierung der respiratorischen Proteine aus A. aeolicus in E.coli." Freiburg : Universität, 2014. http://d-nb.info/1115813617/34.

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Popa, Crina Mihaela. "Clues to the function of AWR effector proteins by expression on heterologous systems = Caracterització funcional de les proteïnes efectores AWR mitjançant l’expressió en sistemes heteròlegs." Doctoral thesis, Universitat de Barcelona, 2015. http://hdl.handle.net/10803/333336.

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The soil-borne plant pathogen Ralstonia solanacearum is the causing agent of bacterial wilt, a devastating disease of wide geographical distribution and host range with enormous economic impact worldwide. To cause disease, R. solanacearum injects a suite of type Ill effector (T3E) proteins into its hosts, from which only a few have been assigned a function, as it is the case for the T3E repertoires of other bacterial pathogens. Using yeast as a model system, we show that expression of the R. solanacearum awr type Ill effector family caused growth inhibition to different extents, and AWR5 displ
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Šiurkus, Juozas [Verfasser], and Peter [Akademischer Betreuer] Neubauer. "Approach for production of sensitive to oxidation and aggregating proteins in E. coli at the example of a heterologous ribonuclease inhibitor / Juozas Šiurkus. Betreuer: Peter Neubauer." Berlin : Universitätsbibliothek der Technischen Universität Berlin, 2013. http://d-nb.info/1031075208/34.

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Matos, Larissa do Rêgo Barros. "Caracterização de proteínas secretadas por Leptospira spp. e sua possível aplicação no diagnóstico da leptospirose." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-09042018-095628/.

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A leptospirose é causada por bactérias do gênero Leptospira e constitui um problema de saúde pública mundial, por acometer humanos e animais. Sua patogênese ainda é pouco esclarecida, especialmente quanto aos processos de invasão, adesão e colonização dos hospedeiros. Recentemente, proteínas secretadas por leptospiras foram identificadas por proteômica e análises in silico, algumas das quais apresentam possível envolvimento no desenvolvimento de quadros hemorrágicos, bem como podem ser possíveis antígenos para diagnóstico. Sendo assim, o presente trabalho propôs a clonagem em vetor de expressã
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Belchior, Gustavo Gross. "Geração de clones de células HEK293 superprodutores de isoformas recombinantes de VEGF-A (Fator de Crescimento Endotelial Vascular A) humano visando à produção de biofármacos para terapia molecular e engenharia tecidual." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-01102014-145602/.

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Os primeiros vasos sanguíneos do embrião de vertebrados, formados de novo a partir de células originadas da mesoderme, originam os vasos linfáticos em um processo denominado vasculogênese. Já no adulto, novos vasos são formados principalmente através da angiogênese (ou linfoangiogênese) a partir da vasculatura pré-existente. Em indivíduos saudáveis, a arquitetura vascular é relativamente estática, sendo que o excesso ou a insuficiência de vasos são comumente relacionados à angiogênese patológica, vinculada a diversas doenças, como câncer, degeneração macular relacionada à idade, isquemia de me
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Varela, Queli Defaveri. "Uso de sistemas virais citoplasmáticos de Saccharomyces cerevisiae para a produção de proteínas heterólogas." reponame:Repositório Institucional da UCS, 2008. https://repositorio.ucs.br/handle/11338/531.

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A produção de proteínas e peptídeos heterólogos representa um dos mais importantes segmentos do setor biotecnológico. Apesar das diferentes tecnologias hoje disponíveis para a (bio) síntese de proteínas e peptídeos (como a síntese química ou o emprego de microrganismos, animais e plantas transgênicos), nenhuma destas é capaz de produzir uma quantidade suficiente de proteínas e peptídeos para suprir as necessidades do mercado por apresentarem altos custos de produção e comercialização. Diante disso, este projeto visou utilizar os sistemas genéticos alternativos ou não convencionais existentes n
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Ngoepe, Mafora Gloria. "Heterologous expression of a Mukwa (pterocarpus angolensis ) seed lectin (Pal) gene in Escherichia coli, Saccharomyces cerevisiae and Yarrowia lipolytica and construction of Pal recombinant vector for expression in Aspergillus niger." Thesis, University of Limpopo (Turfloop Campus), 2011. http://hdl.handle.net/10386/857.

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Thesis (M.Sc. (Microbiology)) --University of Limpopo, 2011<br>Pterocarpus angolensis seed lectin (PAL), a 28 kDa non glycosylated protein, was initially successfully cloned and expressed in E. coli for ease of high protein production. It was discovered, however, as in similar studies that the recombinant PAL yield in E. coli is low and localized intracellularly. This makes extraction even more difficult because most of the protein is lost either when the cell undergoes lysing or when there is incomplete extraction. As a result of the low yields in E. coli, expression vectors were constructed
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Beaumier, Coreen Michele. "Cross-Reactive Memory CD4+ and CD8+ T Cells Alter the Immune Response to Heterologous Secondary Dengue Virus Infections in Mice: A Dissertation." eScholarship@UMMS, 2008. https://escholarship.umassmed.edu/gsbs_diss/350.

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Dengue virus (DENV) infects 50-100 million people worldwide every year and is the causative agent of dengue fever (DF) and the more severe dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). There are four genetically and immunologically distinct DENV serotypes (DENV-1, DENV-2, DENV-3, and DENV-4). Evidence suggests that an increased risk for DHF/DSS during secondary infection with a heterologous DENV serotype is due to an immunopathological response mediated by serotype-cross-reactive memory T cells from the primary infection. Furthermore, epidemiological studies have shown that the seq
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Ronez, Florian. "Exploration fonctionnelle et valorisation industrielle de la protéine de choc thermique bactérienne Lo18." Thesis, Dijon, 2012. http://www.theses.fr/2012DIJOS015.

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La bactérie lactique Oenococcus oeni qui fait partie de la flore d’intérêt du vin, est responsable de la fermentation malolactique. Au cours de son développement dans le vin, Oenococcus oeni est confronté à des conditions physicochimiques drastiques (présence d’éthanol, pH 3,5, basse température, présence de composés soufrés, …). Sa capacité à s’adapter à ces conditions défavorables en fait un bon modèle d’étude de la réponse à de multiples stress chez les bactéries lactiques (Guzzo et al., 2000). L’un des mécanismes de résistance d’O. oeni fait intervenir une protéine de choc thermique de fai
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Raemaekers, Romaan J. M. "Expression of functional plant lectins in heterologous systems." Thesis, Durham University, 2000. http://etheses.dur.ac.uk/4621/.

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The mannose-binding lectin from snowdrop (Galanthus nivalis agglutinin; GNA) was produced in Escherichia coli and purified as a functional protein after denturation/renaturation. Incorporation of the four extra C-terminal residues recently revealed from X-ray crystallographic data demonstrated that these residues increase binding to the glycoprotein carboxypeptidase Y. However, no differences in activities were observed in haemagglutination assays when compared to native GNA and toxicity towards rice brown planthopper (Nilaparvata lugens', BPH) in artificial diet bioassays was unaltered. Site-
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Zhang, Zhigen. "Bioreactor studies of heterologous protein production by recombinant yeast." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq21404.pdf.

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44

Alexander, Clare Lorna. "Factors affecting the production of heterologous protein in saccharomyces cerevisiae." Thesis, Queen's University Belfast, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282046.

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Gong, Fangcheng. "Probing the plant endomembrane-secretory pathway using heterologous membrane protein markers." Diss., The University of Arizona, 1995. http://hdl.handle.net/10150/187119.

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Using gene expression systems developed in Nicotiana tabacum, the transport of various heterologous membrane proteins (SYNVG, IBV M, VSVG, and their derivatives) was studied within the plant secretory pathway. The results of these experiments indicated that the proteins accumulated in various endomembrane compartments of plant cells, but not in those counterparts observed in their native hosts. These results suggest that membrane proteins of mammalian and plant cells share a well-conserved mechanism for ER targeting and translocation, but differences exist in their machinery for retention and
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46

Bauly, James Matthew. "Studies on maize auxin-binding protein in two heterologous expression systems." Thesis, University of Bristol, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265478.

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47

Nosheen, S. (Saman). "Heterologous production and DNA binding activity of Trypanosoma cruzi poly(ADP ribose) polymerase." Master's thesis, University of Oulu, 2013. http://urn.fi/URN:NBN:fi:oulu-201309131688.

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Poly(ADP-ribosylation) is a post-translational covalent modification of proteins and has been involved in processes such as DNA repair and gene expression. The poly(ADP-ribose) polymer (PAR) is mainly catalyzed by a family of enzymes termed poly(ADP-ribose) polymerases (PARPs). TcPARP of Trypanosoma cruzi, appears to play a role in DNA repair mechanism during Chagas’ disease and believed to be involved in controlling different phases of cell growth. In this study, cloning and characterization of mutants of TcPARP is reported. In first phase, five mutants of TcPARP, already cloned in pET-22b+,
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Shahin, Khalid Elsayed Kamal Elsayed. "Development of control strategies for Francisella noatunensis subsp. orientalis in Nile tilapia, Oreochromis niloticus." Thesis, University of Stirling, 2018. http://hdl.handle.net/1893/28046.

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Nile tilapia, Oreochromis niloticus, is one of the most important farmed fish globally. One of the most serious bacterial diseases constraining global tilapia production is Francisellosis caused by Francisella noatunensis subsp. orientalis (Fno). Although outbreaks of Fno are increasing worldwide, there are no licenced commercial vaccines to prevent the disease for use on tilapia farms. Thus, the current treatment of choice is the use of antibiotics combined with increasing water temperature up to 30°C. Studies investigating the diversity of circulating Fno isolates and the immune response of
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BAO, WEIGUO. "Synthese et secretion des proteines heterologues chez la levure." Paris 11, 1999. http://www.theses.fr/1999PA112316.

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Kluyveromyces lactis est souvent choisie comme hote pour la synthese et la secretion de proteines recombinantes, chez le levure. La plupart des premieres etudes etaient axees sur la construction de cassettes portant des genes avec des promoteurs forts ou regules pour obtenir une transcription maximale. Alors qu'un fort niveau de transcription etait atteint dans certains cas, la secretion des proteines n'etait pas proportionnellement activee. Il etait donc suggere qu'une forte transcription n'assurait pas la secretion en grande quantite de la proteine. D'autres etapes limitantes sont aussi impl
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Freeman, Kim. "The effect of blocking selected endocytic mechanisms on heterologous protein secretion in the yeast saccharomyces cerevisiae." University of the Western Cape, 2018. http://hdl.handle.net/11394/6772.

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>Magister Scientiae - MSc<br>The yeast Saccharomyces cerevisiae is considered a good host used for heterologous protein production due to the organism’s microbial safety, rapid growth and eukaryotic post- translational processing. As a fermentative organism, S. cerevisiae is thus not only a useful platform for the production of biopharmaceuticals and industrial enzymes, but also a promising organism for second-generation biofuel production. Substantial effort has been focused on alleviating the many bottlenecks in recombinant gene expression, as well as in the secretory pathway to enhance hete
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