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Journal articles on the topic 'HFF-1'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Schlaepfer, D. D., and H. T. Haigler. "Expression of annexins as a function of cellular growth state." Journal of Cell Biology 111, no. 1 (1990): 229–38. http://dx.doi.org/10.1083/jcb.111.1.229.

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Annexins are a structurally related family of Ca2+ binding proteins of undertermined biological function. Annexin I (also called lipocortin 1) is a substrate for the EGF-stimulated tyrosine kinase and is postulated to be involved in mitogenic signal transduction. To investigate further the involvement of lipocortin 1 in cell proliferation, we measured lipocortin 1 levels in normal diploid human foreskin fibroblasts (HFF) to determine whether its expression changed as a function of growth status. For comparison, the expression of annexin V (also called endonexin II) was measured in HFF cells. E

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Henriksen, Erik J., Mary K. Teachey, Katherine A. Lindborg, Cody J. Diehl, and Alan N. Beneze. "The high-fat-fed lean Zucker rat: a spontaneous isocaloric model of fat-induced insulin resistance associated with muscle GSK-3 overactivity." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 294, no. 6 (2008): R1813—R1821. http://dx.doi.org/10.1152/ajpregu.00178.2008.

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High-fat feeding (HFF) is a well-accepted model for nutritionally-induced insulin resistance. The purpose of this investigation was to assess the metabolic responses of female lean Zucker rats provided regular chow (4% fat) or a high-fat chow (50% fat) for 15 wk. HFF rats spontaneously adjusted food intake so that daily caloric intake matched that of chow-fed (CF) controls. HFF animals consumed more ( P < 0.05) calories from fat (31.9 ± 1.2 vs. 2.4 ± 0.2 kcal/day) and had significantly greater final body weights (280 ± 10 vs. 250 ± 5 g) and total visceral fat (24 ± 3 vs. 10 ± 1 g). Fasting

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Simon, Sozzi, Souza Danilo, Sidhu Karandeep, Garcia Victor, and Tawil Bill. "The effect of omega-3 on muscle and non-muscle cells on a 2D fibronectin and in 3D fibrin constructs." Journal of Applied Biotechnology & Bioengineering 7, no. 3 (2020): 112–19. http://dx.doi.org/10.15406/jabb.2020.07.00224.

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Human Foreskin Fibroblasts (HFF-1) and L6 muscle cells were treated in several conditions to examine the proliferation and adhesion of cells using different extracellular matrix proteins with different concentrations. These proteins consist of Collagen & Fibronectin with concentrations of 5ug/ml and 10ug/ml respectfully. The experiment was conducted and focused on how HFF-1 and L6 cells proliferate in two different conditions over a 7-day period and the results were recorded on Days 1, 3 & 7 (n=9). Different concentrations of Fibronectin and Collagen altered the proliferation of HFF-1

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4

Kusunoki, M., L. H. Storlien, J. MacDessi, et al. "Muscle glucose uptake during and after exercise is normal in insulin-resistant rats." American Journal of Physiology-Endocrinology and Metabolism 264, no. 2 (1993): E167—E172. http://dx.doi.org/10.1152/ajpendo.1993.264.2.e167.

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It is not generally known whether impaired stimulation of muscle glucose metabolism in insulin-resistant states is specific to insulin stimulation. Our aim was to examine whether glucose uptake responded normally to exercise and postexercise recovery in insulin-resistant high-fat-fed (HFF) rats. Three-week HFF or Chow-fed [control (Con)] adult rats were studied 5 days after cannulation. Before, during, or immediately after (recovery) 50 min of treadmill exercise, bolus 2-deoxy-[3H]glucose and [14C]glucose were administered to estimate muscle glucose uptake (R'g) and glycogen incorporation rate

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Moore, Mary Courtney, Renuka Menon, Katie C. Coate, et al. "Diet-induced impaired glucose tolerance and gestational diabetes in the dog." Journal of Applied Physiology 110, no. 2 (2011): 458–67. http://dx.doi.org/10.1152/japplphysiol.00768.2010.

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Glucose metabolism was compared in dogs consuming a chow/meat diet throughout pregnancy (P group, n = 6) and dogs switched to a high-fat/high-fructose (HFF) diet during the 4th–5th gestational week (gestation ≃9 wk; P-HFF group; n = 6). An oral glucose tolerance test (OGTT; 0.9 g/kg) was administered in the 6th–7th gestational week, and a hyperinsulinemic [0–120 min: 1.8 pmol·kg−1·min−1 (low insulin); 120–240 min: 9 pmol·kg−1·min−1 (high insulin)] euglycemic clamp was performed the following week. Nonpregnant (NP) female dogs underwent OGTTs but not clamp studies. All P-HFF dogs exhibited impa

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6

Mindêllo Neto, Ubirajara Ribeiro. "Enraizamento de estacas de pessegueiro em função do uso de ácido indolbutírico e fertilizante orgânico." Revista Brasileira de Fruticultura 27, no. 1 (2005): 92–94. http://dx.doi.org/10.1590/s0100-29452005000100025.

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Este trabalho teve como objetivo avaliar o efeito de concentrações de ácido indolbutírico e do fertilizante orgânico HFF turfa fértil® no enraizamento de estacas semilenhosas do pessegueiro 'Capdeboscq'. Estacas foram tratadas com AIB em concentrações de 500; 1.000; 2.000 e 4.000 mg.L-1 e água destilada como testemunha (0 mg.L-1). Posteriormente, as estacas foram acondicionadas em bandejas de poliestireno expandido com 72 células, contendo como substrato vermiculita, e colocadas em câmara de nebulização intermitente. Aos 30 dias após o início do experimento, foi colocado o fertilizante orgânic

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7

Franco, Evelyn J., Camilly P. Pires de Mello, and Ashley N. Brown. "Antiviral Evaluation of UV-4B and Interferon-Alpha Combination Regimens against Dengue Virus." Viruses 13, no. 5 (2021): 771. http://dx.doi.org/10.3390/v13050771.

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Dengue virus (DENV) is a flavivirus associated with clinical manifestations ranging in severity from self-limiting dengue fever, to the potentially life threatening condition, severe dengue. There are currently no approved antiviral therapies for the treatment of DENV. Here, we evaluated the antiviral potential of four broad-spectrum antivirals, UV-4B, interferon-alpha (IFN), sofosbuvir (SOF), and favipiravir (FAV) against DENV serotype 2 as mono- and combination therapy in cell lines that are physiologically relevant to human infection. Cell lines derived from human liver (HUH-7), neurons (SK

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8

Dalahmeh, Sahar S., Almoayied Assayed, and Ylva Stenström. "Combined Vertical-Horizontal Flow Biochar Filter for Onsite Wastewater Treatment—Removal of Organic Matter, Nitrogen and Pathogens." Applied Sciences 9, no. 24 (2019): 5386. http://dx.doi.org/10.3390/app9245386.

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This study investigated the performance of a combined vertical-horizontal flow biochar filter (VFF-HFF) system in terms of organic matter, total nitrogen (Tot-N), Escherichia coli and Salmonella removal and explored the effects of hydraulic loading rate (HLR) on pollutant removal. The combined VFF-HFF system used biochar as the filter medium and comprised two stacked sections: (i) an aerobic vertical flow filter (VFF) in which the wastewater percolated through the biochar medium in unsaturated mode and (ii) a horizontal flow filter (HFF), in which the biochar was saturated with water and had l

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9

Tunbenjasiri, Keerapach, Thatsanapong Pongking, Chutima Sitthirach, et al. "Metagenomics and metaproteomics alterations are associated with kidney disease in opisthorchiasis hamsters fed a high-fat and high-fructose diet." PLOS ONE 19, no. 5 (2024): e0301907. http://dx.doi.org/10.1371/journal.pone.0301907.

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Background Opisthorchis viverrini (O. viverrini, Ov) infection and consumption of high-fat and high-fructose (HFF) diet exacerbate liver and kidney disease. Here, we investigated the effects of a combination of O. viverrini infection and HFF diet on kidney pathology via changes in the gut microbiome and host proteome in hamsters. Methodology/Principal findings Twenty animals were divided into four groups; 1) fed a normal diet not infected with O. viverrini (normal group), 2) fed an HFF diet and not infected with O. viverrini (HFF), 3) fed a normal diet and infected with O. viverrini (Ov), and

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Ogles, Charles Zachry, Joseph M. Kemble, Amy N. Wright, and Elizabeth A. Guertal. "Evaluation of an Organic Nitrogen Source in a Yellow Squash–Collard Rotation." HortScience 50, no. 1 (2015): 51–58. http://dx.doi.org/10.21273/hortsci.50.1.51.

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In-season nitrogen (N) management is a common challenge in organic vegetable production. This is especially true when using polyethylene mulch combined with fertigation. Soluble organic N sources suitable for fertigation in organic vegetable production are needed. The objective of this research was to evaluate an organic fish fertilizer in a squash/collard rotation and to compare its effectiveness to inorganic sources. A 2-year crop sequence of yellow squash (Cucurbita pepo) and collards (Brassica oleracea var. acephala) was used. To eliminate the rotation order effect, the crops were switched

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11

Chan, Chun-Wai, and Bi-Fong Lin. "Folate Deficiency Enhanced Inflammation and Exacerbated Renal Fibrosis in High-Fat High-Fructose Diet-Fed Mice." Nutrients 15, no. 16 (2023): 3616. http://dx.doi.org/10.3390/nu15163616.

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The prevalence of obesity and chronic kidney disease (CKD) is increasing simultaneously and rapidly worldwide. Our previous study showed that folate deficiency increased lipid accumulation and leptin production of adipocytes. Whether folate plays a role in CKD, particularly obesity-related nephropathy remains unclear. To investigate the effects of folate deficiency on CKD in diet-induced obese mice, four groups of male C57BL/6 mice were fed either a normal-fat diet (NF) with folate (NF+f); NF without folate (NF−f); high-fat high-fructose diet (HFF) with folate (HFF+f); or HFF without folate (H

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12

Cairns, S. P., and A. F. Dulhunty. "Beta-adrenoceptor activation shows high-frequency fatigue in skeletal muscle fibers of the rat." American Journal of Physiology-Cell Physiology 266, no. 5 (1994): C1204—C1209. http://dx.doi.org/10.1152/ajpcell.1994.266.5.c1204.

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The effect of terbutaline (a beta 2-adrenergic agonist) on high-frequency fatigue (HFF) was studied in small bundles of rat soleus muscle fibers. HFF, the decline in force during continuous stimulation (50 Hz for 20 s), was reduced by 10-20% with 10 microM terbutaline. A similar reduction in HFF with 2 mM dibutyryl-adenosine 3',5'-cyclic monophosphate (DBcAMP) implicated adenosine 3',5'-cyclic monophosphate (cAMP) as the second messenger in the terbutaline effect. Sodium (Na-K)-pump inhibition with 1 mM ouabain depressed peak tetanic force but did not significantly alter either the subsequent

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13

Rigotto, Giulia, Barbara Montini, Adriana Mattiolo, et al. "Mechanisms Involved in the Promoting Activity of Fibroblasts in HTLV-1-Mediated Lymphomagenesis: Insights into the Plasticity of Lymphomatous Cells." International Journal of Molecular Sciences 22, no. 19 (2021): 10562. http://dx.doi.org/10.3390/ijms221910562.

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Among the mechanisms leading to progression to Adult T-cell Leukaemia/Lymphoma in Human T-cell Leukaemia Virus type 1 (HTLV-1)-infected subjects, the contribution of stromal components remains poorly understood. To dissect the role of fibroblasts in HTLV-1-mediated lymphomagenesis, transcriptome studies, cytofluorimetric and qRT-PCR analyses of surface and intracellular markers linked to plasticity and stemness in coculture, and in vivo experiments were performed. A transcriptomic comparison between a more lymphomagenic (C91/III) and the parental (C91/PL) cell line evidenced hyperactivation of

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14

Shinojima, Ari, Yoko Ozawa, Atsuro Uchida, et al. "Assessment of Hypofluorescent Foci on Late-Phase Indocyanine Green Angiography in Central Serous Chorioretinopathy." Journal of Clinical Medicine 10, no. 10 (2021): 2178. http://dx.doi.org/10.3390/jcm10102178.

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To assess the hypofluorescent foci (HFF) on late-phase indocyanine green angiography (ICGA) in central serous chorioretinopathy (CSC) using short-wavelength fundus autofluorescence (SW-FAF), near-infrared autofluorescence (NIR-AF), and fluorescein angiography (FA). The HFF area on late-phase ICGA for at least 20 min was compared with the area of abnormal foci on SW-FAF, NIR-AF, and FA. In 14 consecutive patients (12 men, including 1 with bilateral CSC; and 2 women with unilateral CSC), four kinds of images of 27 eyes were acquired. The mean age ± standard deviation (range) was 46 ± 9.2 years (

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15

Zhao, Pinsong, F. S. Liu, Qifan Cui, Hassen M. Yesuf, and Hong Wu. "Structure and Color Gradients of Ultradiffuse Galaxies in Distant Massive Galaxy Clusters." Astrophysical Journal 960, no. 1 (2023): 9. http://dx.doi.org/10.3847/1538-4357/acfd90.

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Abstract We have measured structural parameters and radial color profiles of 108 ultradiffuse galaxies (UDGs), carefully selected from six distant massive galaxy clusters in the Hubble Frontier Fields (HFF) in a redshift range from 0.308 to 0.545. Our best-fitting GALFIT models show that the HFF UDGs have a median Sérsic index of 1.09, which is close to 0.86 for local UDGs in the Coma cluster. The median axis-ratio value is 0.68 for HFF UDGs and 0.74 for Coma UDGs, respectively. The structural similarity between HFF and Coma UDGs suggests that they are the same kind of galaxies seen at differe

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16

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Wound Healing Activity of Consciousness Energy Healing Treatmenton HFF-1 Cells and DMEM Using Scratch Assay." Investigative Dermatology and Venereology Research 4, no. 1 (2018): 50–54. https://doi.org/10.5281/zenodo.3374350.

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The wound healing activity using scratch assay is considered as a convenient in vitro tool for the assessment of wound healing. The present study deals with the optimization of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco’s Modified Eagle Medium) using scratch assay against positive control, recombinant Human Epidermal Growth Factor (Hu EGF, 30 ng/mL). This methodwas used for the determination of cell proliferation and migration of fibroblast quantitatively in the scratched

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17

Holzer, Isabelle, Oksana Desiatkina, Nicoleta Anghel, et al. "Synthesis and Antiparasitic Activity of New Trithiolato-Bridged Dinuclear Ruthenium(II)-arene-carbohydrate Conjugates." Molecules 28, no. 2 (2023): 902. http://dx.doi.org/10.3390/molecules28020902.

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Eight novel carbohydrate-tethered trithiolato dinuclear ruthenium(II)-arene complexes were synthesized using CuAAC ‘click’ (Cu(I)-catalyzed azide-alkyne cycloaddition) reactions, and there in vitro activity against transgenic T. gondii tachyzoites constitutively expressing β-galactosidase (T. gondii β-gal) and in non-infected human foreskin fibroblasts, HFF, was determined at 0.1 and 1 µM. When evaluated at 1 µM, seven diruthenium-carbohydrate conjugates strongly impaired parasite proliferation by >90%, while HFF viability was retained at 50% or more, and they were further subjected to the

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Fowler, P. A., P. Cunningham, M. Fraser, et al. "Circulating gonadotrophin surge-attenuating factor from superovulated women suppresses in vitro gonadotrophin-releasing hormone self-priming." Journal of Endocrinology 143, no. 1 (1994): 45–54. http://dx.doi.org/10.1677/joe.0.1430045.

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Abstract A penfusion system based on ovine pituitary tissue explants was used to investigate the effects of follicular fluid (hFF) and serum from superovulated women on pituitary responsiveness to gonadotrophin-releasing hormone (GnRH). The specific aims of the study were to determine both if gonadotrophin surge-attenuating factor (GnSAF) bioactivity is present in the peripheral circulation as well as in the follicles of superovulated women and if GnSAF suppresses GnRH self-priming in vitro. Two pulses of GnRH, 1 h apart, produced marked peaks in LH secreted from control chambers, with GnRH se

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Mazzoli, Arianna, Raffaella Crescenzo, Luisa Cigliano, et al. "Early Hepatic Oxidative Stress and Mitochondrial Changes Following Western Diet in Middle Aged Rats." Nutrients 11, no. 11 (2019): 2670. http://dx.doi.org/10.3390/nu11112670.

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To assess the effect of 4 weeks of high fat-high fructose feeding on whole body composition, energy balance, specific markers of oxidative stress and inflammation, and insulin sensitivity in the liver of middle-aged rats, rats (1 year) were fed a diet rich in saturated fatty acids and fructose (HFF rats), mimicking the “Western diet”, and compared with rats of the same age that were fed a low fat diet (LF rats). HFF rats exhibited a significant increase in the gain of body weight, energy, and lipids compared to LF rats. HFF rats also showed hepatic insulin resistance, together with an increase

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She, Jie-Mei, Hui-Hui Yuan, Jin Zhang, Zhao-Nian Si, and Min-Bo Lan. "Protective effect of the Rubus chingii Hu. fruit extract on ultraviolet B-induced photoaging via suppression of mitogen-activated protein kinases (MAPKs) in vitro." Archives of Biological Sciences 71, no. 3 (2019): 541–50. http://dx.doi.org/10.2298/abs190321038s.

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Rubus chingii Hu. is an important traditional Chinese medicine with a beneficial effect on the kidney. Traditional Chinese medicine theory believes that supporting the kidney has a key role in anti-aging. To the best of our knowledge, the anti-photoaging activity of Rubus chingii Hu. has not been investigated. We investigated the anti-photoaging effects and the underlying mechanism of the ethyl acetate fraction of Rubus chingii Hu. fruit ethanol extract (E-EA) in UVB-induced human fibroblasts (HFF-1). Our results show that E-EA (10-40 ?g/mL) displayed preventive and therapeutic effects on UVB-

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Clark, E. A., K. H. Grabstein, A. M. Gown, et al. "Activation of B lymphocyte maturation by a human follicular dendritic cell line, FDC-1." Journal of Immunology 155, no. 2 (1995): 545–55. http://dx.doi.org/10.4049/jimmunol.155.2.545.

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Abstract Previously, we described the characteristics of a cell line that is derived from a low density fraction of human tonsillar cells and, on the basis of a number of criteria, is related to follicular dendritic cells (FDC). This line, FDC-1, binds B lymphocytes and not T lymphocytes, and promotes anti-Ig- or anti-CD40-induced B cell proliferation. In this work, we show that culturing B cells with small numbers of FDC-1 cells leads to significant production of IL-6 and of both IgM and IgG. As few as 50 to 100 FDC-1 augmented B cell IgM production by 10- to 100-fold. Although fixed FDC-1 ce

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, and Snehasis Jana. "Assessment of Anti-Ageing Potential of Consciousness Energy Healing Treated DMEM and HFF-1 Cells using Cellular Proliferation and Collagen Metabolism Assays." Letters in Health and Biological Sciences 4, no. 1 (2019): 1–6. https://doi.org/10.15436/2475-6245.19.1977.

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Skin health and aging are the complex biological process influenced by several intrinsic (or endogenous) and extrinsic (or exogenous) factors. Various skin-based therapies are currently available to rejuvenate the skin, but they might be related with some side-effects such as scarring. The objective of the present study was to evaluate the effect of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the human foreskin fibroblast (HFF-1) cell line and Dulbecco’s Modified Eagle Medium (DMEM) for skin health parameters like cell proliferation and synthesis of collagen. The

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Qian, Qun, Ni Zhu, Wenzhe Li, et al. "Human Umbilical Mesenchymal Stem Cells-Derived Microvesicles Attenuate Formation of Hypertrophic Scar through Multiple Mechanisms." Stem Cells International 2023 (July 15, 2023): 1–15. http://dx.doi.org/10.1155/2023/9125265.

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Mesenchymal stem cells and the derived extracellular microvesicles are potential promising therapy for many disease conditions, including wound healing. Since current therapeutic approaches do not satisfactorily attenuate or ameliorate formation of hypertrophic scars, it is necessary to develop novel drugs to achieve better outcomes. In this study, we investigated the effects and the underlying mechanisms of human umbilical mesenchymal stem cells (HUMSCs)-derived microvesicles (HUMSCs-MVs) on hypertrophic scar formation using a rabbit ear model and a human foreskin fibroblasts (HFF) culture mo

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Hasegawa, Keisuke, Sung Jin Ryu, and Petr Kaláb. "Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells." Journal of Cell Biology 200, no. 2 (2013): 151–61. http://dx.doi.org/10.1083/jcb.201206142.

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Many mitotic factors were shown to be activated by Ran guanosine triphosphatase. Previous studies in Xenopus laevis egg extracts and in highly proliferative cells showed that mitotic chromosomes were surrounded by steep Ran guanosine triphosphate (GTP) concentration gradients, indicating that RanGTP-activated factors promote spindle assembly around chromosomes. However, the mitotic role of Ran in normal differentiated cells is not known. In this paper, we show that although the steep mitotic RanGTP gradients were present in rapidly growing cell lines and were required for chromosome congressio

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Cerf, M. E., K. Williams, X. I. Nkomo, et al. "Islet cell response in the neonatal rat after exposure to a high-fat diet during pregnancy." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 288, no. 5 (2005): R1122—R1128. http://dx.doi.org/10.1152/ajpregu.00335.2004.

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Although pancreatic β-cells are capable of adapting their mass in response to insulin requirements, evidence has shown that a dietary insult could compromise this ability. Fetal malnutrition has been linked to low birth weight and the development of type 2 diabetes later in life, while reduced β-cell mass has been reported in adult rats fed a high-fat diet (HFD). Reported here are the effects of exposure to a HFD, during different periods of gestation, on neonatal rat weight and β- and α-cell development. The experimental groups were composed of neonatal offspring obtained from Wistar rats fed

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Jana, Snehasis, and Mahendra Kumar Trivedi. "Wound Healing Activity of Consciousness Energy Healing Treatmenton HFF-1 Cells and DMEM Using Scratch Assay." Investigative Dermatology and Venereology Research 4, no. 1 (2018): 50–54. http://dx.doi.org/10.15436/2381-0858.18.2036.

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The wound healing activity using scratch assay is considered as a convenient in vitro tool for the assessment of wound healing. The present study deals with the optimization of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) using scratch assay against positive control, recombinant Human Epidermal Growth Factor (Hu EGF, 30 ng/mL). This methodwas used for the determination of cell proliferation and migration of fibroblast quantitatively in the scratched wounded a

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27

Elsayed, Hassan Reda Hassan, Mohammad El-Nablaway, Basma Adel Khattab, et al. "Independent of Calorie Intake, Short-term Alternate-day Fasting Alleviates NASH, With Modulation of Markers of Lipogenesis, Autophagy, Apoptosis, and Inflammation in Rats." Journal of Histochemistry & Cytochemistry 69, no. 9 (2021): 575–96. http://dx.doi.org/10.1369/00221554211041607.

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Non-alcoholic steatohepatitis (NASH) is a worldwide health problem. Alternate-day fasting (ADF), although thought to be aggressive, has proven safety and efficacy. We aimed to evaluate the effect of short-term ADF against already established high-fat-fructose (HFF)-induced NASH, independent of the amount of calorie intake, and to study the effect of ADF on lipogenesis, apoptosis, and hepatic inflammation. Male Sprague Dawley rats were divided into two groups: (1) negative control and (2) NASH group fed on HFF for 9 weeks, and then randomized into two subgroups of either HFF alone or with ADF p

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Mazzoli, Arianna, Maria Stefania Spagnuolo, Cristina Gatto, et al. "Adipose Tissue and Brain Metabolic Responses to Western Diet—Is There a Similarity between the Two?" International Journal of Molecular Sciences 21, no. 3 (2020): 786. http://dx.doi.org/10.3390/ijms21030786.

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Dietary fats and sugars were identified as risk factors for overweight and neurodegeneration, especially in middle-age, an earlier stage of the aging process. Therefore, our aim was to study the metabolic response of both white adipose tissue and brain in middle aged rats fed a typical Western diet (high in saturated fats and fructose, HFF) and verify whether a similarity exists between the two tissues. Specific cyto/adipokines (tumor necrosis factor alpha (TNF-α), adiponectin), critical obesity-inflammatory markers (haptoglobin, lipocalin), and insulin signaling or survival protein network (i

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Desiatkina, Oksana, Martin Mösching, Nicoleta Anghel, et al. "New Nucleic Base-Tethered Trithiolato-Bridged Dinuclear Ruthenium(II)-Arene Compounds: Synthesis and Antiparasitic Activity." Molecules 27, no. 23 (2022): 8173. http://dx.doi.org/10.3390/molecules27238173.

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Aiming toward compounds with improved anti-Toxoplasma activity by exploiting the parasite auxotrophies, a library of nucleobase-tethered trithiolato-bridged dinuclear ruthenium(II)-arene conjugates was synthesized and evaluated. Structural features such as the type of nucleobase and linking unit were progressively modified. For comparison, diruthenium hybrids with other type of molecules were also synthesized and assessed. A total of 37 compounds (diruthenium conjugates and intermediates) were evaluated in a primary screening for in vitro activity against transgenic Toxoplasma gondii tachyzoit

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Fowler, P. A., C. Townsend, I. E. Messinis, P. Cunningham, and A. Templeton. "Gonadotrophin surge-attenuating factor attenuates in-vitro LH secretion induced by gonadotrophin-releasing hormone from cultured ovine pituitary cells only during the breeding season." Journal of Endocrinology 135, no. 2 (1992): 221–27. http://dx.doi.org/10.1677/joe.0.1350221.

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ABSTRACT Primary cultures of ovine pituitaries from adult ewes were used to investigate aspects of gonadotrophin surge-attenuating factor (GnSAF) bioactivity in human follicular fluid (hFF) from superovulated women. During the autumn and first half of the winter, LH secretion induced by gonadotrophinreleasing hormone (GnRH) was markedly reduced (43·5 ± 5·2% of control GnRH-induced LH secretion) by incubation for 48 h with steroid-free hFF. For the rest of the year, treatment with the same batch of steroid-free hFF resulted in non-significant reduction or stimulation of GnRH-induced LH secretio

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Iffatuzzahra', Yumna Nabilah, Diah Kartika Wardani, Andika Gundawa Hidayat, Ulinuha Farah Makhabati, and Haris Setiawan. "Effects of Wedelia Ethanol Extract on Sperm Quality and Blood Cholesterol Levels in Obese Rats." Jurnal Biodjati 9, no. 2 (2024): 321–33. https://doi.org/10.15575/biodjati.v9i2.37673.

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Obesity is a complex, multifactorial condition contributing to male infertility by increasing oxidative stress, which negatively affects reproductive hormones, sperm quality, and blood cholesterol levels. The Wedelia plant (Sphagneticola trilobata (L.) Pruski) is a natural herbaceous plant containing flavonoids and tannins, known for enhancing sperm quality and inhibiting cholesterol synthesis in the blood. This study aims to determine the potential of Wedelia in improving sperm quality and regulating blood cholesterol levels. The study used 24 male Wistar rats divided into four groups, consis

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SOARES, Priscilla Barbosa Ferreira, Aletheia Moraes ROCHA, Manuella Verdinelli de Paula REIS, Camilla Christian Gomes MOURA, and Carlos José SOARES. "Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study." Revista de Odontologia da UNESP 45, no. 4 (2016): 234–39. http://dx.doi.org/10.1590/1807-2577.09216.

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Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT forma

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Ortiz Monsalve, Camilo, Jorge Mario Guerra González, and Marisol Jaramillo Grajales. "Immobilization of DNA probes on a high frequency piezoelectric biosensor." DYNA 87, no. 212 (2020): 163–68. http://dx.doi.org/10.15446/dyna.v87n212.82309.

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In recent years, researchers have taken to biosensors as effective tools for detection due to their portability, low-cost, fast response, and practicality. Piezoelectricity gave way to quartz crystal microbalances (QCM), of which high-frequency QCMs (HFF-QCM 100MHz) are still being researched. In this paper, we use DNA immobilization on a HFF-QCM via self-assembled monolayers (SAM) technique. Immobilization was initially verified with ATR-FTIR. Then, DNA was immobilized in real time on the HFF-QCM crystals. A variation in the phase of the signal suggests fixation of DNA to the surface, in acco

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Somassè, Yassinmè Elysée, Michèle Dramaix, Boncana Traoré, et al. "The WHO recommendation of home fortification of foods with multiple-micronutrient powders in children under 2 years of age and its effectiveness on anaemia and weight: a pragmatic cluster-randomized controlled trial." Public Health Nutrition 21, no. 7 (2018): 1350–58. http://dx.doi.org/10.1017/s1368980017003858.

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AbstractObjectiveTo assess under real community settings the effectiveness of the WHO strategy of home fortification of foods (HFF) with multiple-micronutrient powders on Hb change, anaemia and weight in children.DesignA pragmatic cluster-randomized controlled trial.SettingForty villages in the Nioro Circle in Mali and 722 children aged 6–23 months were randomized to the intervention or control group. The intervention consisted of a daily dose of multiple-micronutrient powder for 3 months; in the control group, no supplement was given. In both groups, mothers received group education on child

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Ruíz-Baltazar, Álvaro de Jesús, Simón Yobanny Reyes-López, Néstor Méndez-Lozano, and Karla Juárez-Moreno. "Evaluation of Superparamagnetic Fe3O4-Ag Decorated Nanoparticles: Cytotoxicity Studies in Human Fibroblasts (HFF-1) and Breast Cancer Cells (MCF-7)." Applied Sciences 14, no. 15 (2024): 6750. http://dx.doi.org/10.3390/app14156750.

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This study investigates the cytotoxicity profile of superparamagnetic Fe3O4-Ag decorated nanoparticles against human fibroblasts (HFF-1) and breast cancer cells (MCF-7). The nanoparticles underwent comprehensive characterization employing scanning electron microscopy (SEM), X-ray diffraction (XRD) analysis, X-ray photoelectron spectroscopy (XPS), and magnetic assays including hysteresis curves and zero-field-cooled (ZFC) plots. The nanoparticles exhibited superparamagnetic behavior as evidenced by magnetic studies. Cytotoxicity assays demonstrated that both HFF-1 and MCF-7 cells maintained nea

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Estimation of Collagen Synthesis in Human Dermal Fibroblast Cells: an Impact of Consciousness Energy Healing Treatment." International Journal of Research Studies in Medical and Health Sciences 3, no. 9 (2018): 18–24. https://doi.org/10.5281/zenodo.2558156.

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Human dermal fibroblasts are defined for skin strength and resilience by continuously secreting extracellular matrix (ECM) components, which are responsible for promoting skin rejuvenation and remodeling. Precisely, the branched collagen fibrils provide the skin with its shape and firmness. The objective of the present study was to assess the potential of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) for evaluation of skin health parameters such as pro

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Estimation of Collagen Synthesis in Human Dermal Fibroblast Cells: an Impact of Consciousness Energy Healing Treatment." International Journal of Research Studies in Medical and Health Sciences 3, no. 9 (2018): 18–24. https://doi.org/10.5281/zenodo.2564484.

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Human dermal fibroblasts are defined for skin strength and resilience by continuously secreting extracellular matrix (ECM) components, which are responsible for promoting skin rejuvenation and remodeling. Precisely, the branched collagen fibrils provide the skin with its shape and firmness. The objective of the present study was to assess the potential of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) for evaluation of skin health parameters such as pro

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Hegarty-Diaz, Bonnie P., Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, and Gopal Nayak. "Skin Photo-Protective and Anti-ageing Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation." Journal of Diseases and Medicinal Plants 3, no. 3 (2017): 65–75. https://doi.org/10.5281/zenodo.854496.

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The objective of this study was to evaluate the impact of The Trivedi Effect®- Consciousness Energy Healing Treatment based herbomineral formulation on various skin parameters using HFF-1, HaCaT, and B16-F1 cell lines. The test formulation was composed of minerals (zinc, selenium, and molybdenum), and L-ascorbic acid along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). Test formulation and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment by Bonnie P. Hegarty-Diaz and denoted as treated, while other part was coded as untreat

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Wei, Mingjun, Lakshmi Jeevithan, Na Li, et al. "Stem-Cell-Regenerative and Protective Effects of Squid (Symplectoteuthis oualaniensis) Skin Collagen Peptides against H2O2-Induced Fibroblast Injury." Marine Drugs 22, no. 6 (2024): 255. http://dx.doi.org/10.3390/md22060255.

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Recently, there has been a growing interest in collagen peptides derived from marine sources for their notable ability to protect skin cells against apoptosis induced by oxidants. Therefore, the current study aimed to investigate the fundamental properties of collagen peptides, including their physicochemical, thermal, structural, stem-cell-regenerative, and skin-cell-protective effects, in comparison to commercial collagen peptides. The acid-soluble (ASC) and pepsin-soluble (PSC) collagens exhibited three distinct bands on SDS-PAGE, namely α (α1 and α2), β, and γ chains, confirming a type I p

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Desiatkina, Oksana, Serena K. Johns, Nicoleta Anghel, et al. "Synthesis and Antiparasitic Activity of New Conjugates—Organic Drugs Tethered to Trithiolato-Bridged Dinuclear Ruthenium(II)–Arene Complexes." Inorganics 9, no. 8 (2021): 59. http://dx.doi.org/10.3390/inorganics9080059.

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Tethering known drugs to a metalorganic moiety is an efficient approach for modulating the anticancer, antibacterial, and antiparasitic activity of organometallic complexes. This study focused on the synthesis and evaluation of new dinuclear ruthenium(II)–arene compounds linked to several antimicrobial compounds such as dapsone, sulfamethoxazole, sulfadiazine, sulfadoxine, triclosan, metronidazole, ciprofloxacin, as well as menadione (a 1,4-naphtoquinone derivative). In a primary screen, 30 compounds (17 hybrid molecules, diruthenium intermediates, and antimicrobials) were assessed for in vitr

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Makumbe, Peter, Gift Chikorowondo, Pride Canisia Dzamara, Henry Ndaimani, and Edson Gandiwa. "Effects of Fire Frequency on Woody Plant Composition and Functional Traits in a Wet Savanna Ecosystem." International Journal of Ecology 2020 (November 3, 2020): 1–11. http://dx.doi.org/10.1155/2020/1672306.

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The objective of this study was to assess the effect of fire frequency on vegetation taxonomic and functional diversity in a wet savanna ecosystem, eastern Zimbabwe. The study area was stratified into three fire frequency regimes using a 15-year fire history (2000–2014) across the landscape: high (HFF: burnt every 1-2 years), medium (MFF: burnt every 3-4 years), and low (LFF: burnt every 5-6 years). Data were collected from a total of 30 plots measuring 20 m × 20 m each between March and May 2018. In each plot, we recorded tree maximum height (Hmax), woody plant density, basal diameter, respro

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Deng, Ping, Alison N. Beloshapka, Brittany M. Vester Boler, and Kelly S. Swanson. "Dietary fibre fermentability but not viscosity elicited the ‘second-meal effect’ in healthy adult dogs." British Journal of Nutrition 110, no. 5 (2013): 960–68. http://dx.doi.org/10.1017/s0007114513000020.

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The present study evaluated the effects of fibre fermentability and viscosity in a morning meal on glucose, insulin and glucagon-like peptide-1 (GLP-1) responses to a glucose challenge later in the day in six healthy female dogs. For this purpose, two Latin square design experiments were performed. In Expt 1, dogs were fed a low-fibre (LF; 1 % Solka-Floc (International Fiber Corporation) and 1 % soya hulls) diet, a low-fermentable fibre (LFF; 5 % Solka-Floc (International Fiber Corporation) and 3 % soya hulls) diet or a high-fermentable fibre (HFF; 5 % pectin and 3 % short-chain fructo-oligosa

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Grünebach, Frank, Markus M. Weck, Silke Appel, et al. "hDectin-1 Mediates Cross-Presentation of Epitopes Via the Cytosolic Pathway." Blood 110, no. 11 (2007): 2313. http://dx.doi.org/10.1182/blood.v110.11.2313.2313.

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Abstract Human (h)Dectin-1 is a member of the C-type-lectin-like receptor family that was shown to be the major receptor for fungal β-glucans and to play an important role in cellular responses mediated by these carbohydrates. It is mainly expressed on human DCs and macrophages. In our study, we observed that activation of monocyte-derived dendritic cells (MDCs) with TLR3 ligand (poly I:C) but not with TLR ligand 7/8 (R848) resulted in down-regulation of hDectin-1 expression and this down-regulation correlated with a reduced uptake of apoptotic cells in phagocytosis assays. In order to analyze

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Al Juffali, Lobna Abdullah, Peter Knapp, Sinaa Al-Aqeel, and Margaret C. Watson. "Medication safety problems priorities in community pharmacy in Saudi Arabia: a multi-stakeholder Delphi study using the human factors framework." BMJ Open 9, no. 11 (2019): e032419. http://dx.doi.org/10.1136/bmjopen-2019-032419.

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AimTo achieve multi-stakeholder consensus and prioritisation of medication safety problems in community pharmacies in Saudi Arabia.Design and interventionA theoretically-underpinned, three-round Delphi study.SettingSaudi Arabia.ParticipantsPatients and public (pharmacy users), pharmacy-related professionals (policymakers, academics, medication safety officers and pharmacy owners) and community pharmacists.MethodsRound 1 comprised 84 statements derived from a qualitative study. The items were grouped according to the Human Factors Framework (HFF). Rounds 1 and 2 aimed to achieve consensus, 6-po

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Alomari, Munther, Rabindran Jermy Balasamy, Dana Almohazey, et al. "Nile Red-Poly(Methyl Methacrylate)/Silica Nanocomposite Particles Increase the Sensitivity of Cervical Cancer Cells to Tamoxifen." Polymers 12, no. 7 (2020): 1516. http://dx.doi.org/10.3390/polym12071516.

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Tamoxifen (TAM) is a hormonal drug and is mainly used as an anti-estrogen in breast cancer patients. TAM binds to estrogen receptors (ERs), resulting in inhibition of estrogen signaling pathways and thus, a downregulation of cell proliferation. Cancer cells with negative or low ER expression will not uptake TAM and will show low response. Poly (methyl methacrylate) (PMMA) nanoparticles were prepared using surfactant-free emulsion polymerization, then were loaded with Nile red (NR), which resulted in PMMA-NR. To enhance TAM delivery to cervical cancer cells (HELA), which is considered ER-negati

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Bosch, Guido, Adronie Verbrugghe, Myriam Hesta, et al. "The effects of dietary fibre type on satiety-related hormones and voluntary food intake in dogs." British Journal of Nutrition 102, no. 2 (2009): 318–25. http://dx.doi.org/10.1017/s0007114508149194.

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Depending on type and inclusion level, dietary fibre may increase and maintain satiety and postpone the onset of hunger. This 7-week study evaluated the effect of fibre fermentability on physiological satiety-related metabolites and voluntary food intake (VFI) in dogs. Sixteen healthy adult dogs were fed a low-fermentable fibre (LFF) diet containing 8·5 % cellulose or a high-fermentable fibre (HFF) diet containing 8·5 % sugarbeet pulp and 2 % inulin. Large intestinal fibre degradation was evaluated by apparent faecal digestibility of nutrients and faecal SCFA and NH3 concentrations. Postprandi

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Trivedi, Mahendra Kumar, Dahryn Trivedi, Alice Branton, Gopal Nayak, Sambhu Charan Mondal, and Snehasis Jana. "Assessment of Anti-Ageing Potential of Consciousness Energy Healing Treated DMEM and HFF-1 Cells using Cellular Proliferation and Collagen Metabolism Assays." Lettters in Health and Biological Sciences 4, no. 1 (2019): 1–6. http://dx.doi.org/10.15436/2475-6245.19.1977.

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Skin health and aging are the complex biological process influenced by several intrinsic (or endogenous) and extrinsic (or exogenous) factors. Various skin-based therapies are currently available to rejuvenate the skin, but they might be related with some side-effects such as scarring. The objective of the present study was to evaluate the effect of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the human foreskin fibroblast (HFF-1) cell line and Dulbecco’s Modified Eagle Medium (DMEM) for skin health parameters like cell proliferation and synthesis of collagen. The rate of ce

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Small, EJ, JA Katzmann, RP Tracy, OD Ratnoff, GH Jr Goldsmith, and B. Everson. "A monoclonal antibody that inhibits activation of human Hageman factor (factor XII)." Blood 65, no. 1 (1985): 202–10. http://dx.doi.org/10.1182/blood.v65.1.202.202.

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Abstract A monoclonal antibody to human Hageman factor (HF, factor XII) was derived from BALB/c mouse spleen cells fused with NS-1 mouse myeloma cells. This antibody, purified from ascites fluid, reacted with HF to inhibit the activation of HF, purified or in normal pooled plasma, as measured by a coagulation assay. The antibody did not inhibit the coagulant activity of activated HF. The antibody also inhibited the generation of amidolytic activity in HF-ellagic acid mixtures, but failed to inhibit the amidolytic properties of the carboxy-terminal fragment of HF (HFf). Amidolytic activity, abs

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Small, EJ, JA Katzmann, RP Tracy, OD Ratnoff, GH Jr Goldsmith, and B. Everson. "A monoclonal antibody that inhibits activation of human Hageman factor (factor XII)." Blood 65, no. 1 (1985): 202–10. http://dx.doi.org/10.1182/blood.v65.1.202.bloodjournal651202.

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A monoclonal antibody to human Hageman factor (HF, factor XII) was derived from BALB/c mouse spleen cells fused with NS-1 mouse myeloma cells. This antibody, purified from ascites fluid, reacted with HF to inhibit the activation of HF, purified or in normal pooled plasma, as measured by a coagulation assay. The antibody did not inhibit the coagulant activity of activated HF. The antibody also inhibited the generation of amidolytic activity in HF-ellagic acid mixtures, but failed to inhibit the amidolytic properties of the carboxy-terminal fragment of HF (HFf). Amidolytic activity, absent in an

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Fowler, P. A., M. Fraser, P. Cunningham, et al. "Higher gonadotrophin surge-attenuating factor bioactivity is found in small follicles from superovulated women." Journal of Endocrinology 143, no. 1 (1994): 33–44. http://dx.doi.org/10.1677/joe.0.1430033.

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Abstract Ovine and rat pituitary bioassays for gonadotrophin surgeattenuating factor (GnSAF) were utilized to determine whether the level of GnSAF bioactivity in pooled human follicular fluid (hFF) from superovulated women varied according to follicle diameter (≤11 mm, 12–15 mm and 16–21 mm follicles examined using the ovine bioassay, or ≤10 mm, 11–13 mm, 14–17 mm, 18–20 mm, 21–24 mm and ≥ 25 mm follicles examined using the rat bioassay). When tested using dispersed ovine pituitary cells, GnSAF bioactivity, expressed in terms of the reduction in gonadotrophin-releasing hormone (GnRH)-induced L

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