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Journal articles on the topic 'HFOB'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Li, Zhongyong, Zhiyi Zhou, Marnie M. Saunders, and Henry J. Donahue. "Modulation of connexin43 alters expression of osteoblastic differentiation markers." American Journal of Physiology-Cell Physiology 290, no. 4 (2006): C1248—C1255. http://dx.doi.org/10.1152/ajpcell.00428.2005.

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Gap junctional channels between cells provide a pathway for exchange of regulatory ions and small molecules. We previously demonstrated that expression of connexins and cell-to-cell communication parallel osteoblastic differentiation and that nonspecific pharmacological inhibitors of gap junctional communication inhibit alkaline phosphatase activity. In this study, we stably transfected connexin (Cx)43 antisense cDNA into the immortalized human fetal osteoblastic cell line hFOB 1.19 (hFOB/Cx43−). hFOB/Cx43− cells express lower levels of Cx43 protein and mRNA and display a 50% decrease in gap j

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Xiao, Mang, Cynthia E. Inal, and Mark H. Whitnall. "NF-kappa B Plays a Key Role in the Hematopoietic Niche Function of Osteoblast after Ionizing Radiation." Blood 110, no. 11 (2007): 4091. http://dx.doi.org/10.1182/blood.v110.11.4091.4091.

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Abstract Recent studies demonstrated that osteoblasts in the endosteal region of bone are a key cellular component of the hematopoietic niche that directly regulates hematopoietic stem cell (HSC) survival, proliferation and differentiation. In experiments with the human fetal osteoblast cell line hFOB1.19, we found survival of gamma-irradiated primary human hematopoietic CD34+ cells was significantly enhanced by co-culture with hFOB cells or by conditioned medium (CM) from hFOB cells, as shown by increased colony efficiency and colony size. In clonogenic assays of CD34+ cells starting with 5 ×

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3

Jablonská, Eva, Lucie Mrázková, Jiří Kubásek, et al. "Characterization of hFOB 1.19 Cell Line for Studying Zn-Based Degradable Metallic Biomaterials." Materials 17, no. 4 (2024): 915. http://dx.doi.org/10.3390/ma17040915.

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In vitro testing is the first important step in the development of new biomaterials. The human fetal osteoblast cell line hFOB 1.19 is a very promising cell model; however, there are vast discrepancies in cultivation protocols, especially in the cultivation temperature and the presence of the selection reagent, geneticin (G418). We intended to use hFOB 1.19 for the testing of Zn-based degradable metallic materials. However, the sensitivity of hFOB 1.19 to zinc ions has not yet been studied. Therefore, we compared the toxicity of zinc towards hFOB 1.19 under different conditions and compared it

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Song, Jiangtao, Wenrong Song, and Lei Zhang. "LncRNA RP1-85F18.6 affects osteoblast cells by regulating the cell cycle." Open Life Sciences 15, no. 1 (2020): 951–58. http://dx.doi.org/10.1515/biol-2020-0090.

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AbstractA lncRNA RP1-85F18.6 was reported to affect cell growth by regulating the cell cycle. Here we tested whether it affects the proliferation of osteoblast cells by regulating the cell cycle. We determined the expression of RP1-85F18.6 in two osteoblast cell lines hFOB and HOB by qPCR. Then we knocked down or overexpressed RP1-85F18.6 in hFOB and tested the alteration of viability, cell cycle, and cell cycle regulatory proteins. Results showed that both hFOB and HOB expressed RP1-85F18.6. The knockdown of RP1-85F18.6 decreased the viability of hFOB, while the overexpression of it increased

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Hameed, Hanan Ali, Haider Ali Hasan, Norhayati Luddin, Adam Husein, Azirrawani Ariffin, and Mohammad Khursheed Alam. "Osteoblastic Cell Responses of Copper Nanoparticle Coatings on Ti-6Al-7Nb Alloy Using Electrophoretic Deposition Method." BioMed Research International 2022 (April 19, 2022): 1–11. http://dx.doi.org/10.1155/2022/3675703.

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Aim. To investigate and compare the cell cytotoxicity, proliferation, cell attachment, and morphology of human fetal osteoblasts (hFOB) cells of coated samples (titanium nanocopper (Ti Cu), titanium nanohydroxyapatite (Ti HA) and titanium nanocopper ion doped hydroxyapatite (Ti Cu/HA) and uncoated samples (Ti) in order to assess the suitability of these surface modifications on Ti-6Al-7Nb for dental implant application. Materials and Methods. The cytotoxicity was studied by examining the hFOB cell response by MTT assessment. The cell morphology was evaluated by inverted microscopy and observed

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Aditama, Agnis Pondineka Ria, Burhan Ma’arif, Hening Laswati, and Mangestuti Agil. "The effect of ethyl acetate fraction of Marsilea crenata presl. leaves in increasing osterix expression in hFOB 1.19 cells." International journal of health sciences 6, no. 2 (2022): 789–96. http://dx.doi.org/10.53730/ijhs.v6n2.8096.

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Marsilea crenata Presl. leaves contain phytoestrogens that is suspected to play a role in increasing bone formation. Bone formation activity is defined by the expression of Osterix, a transcription factor that plays a key role in bone development. The aim of this study is to show that the ethyl acetate fraction of Marsilea crenata Presl. leaves can increase bone formation process in hFOB 1.19 cells in a TNF-α dependent manner, by measurement of Osterix. The hFOB 1.19 cells were grown in 24-well microplates and treated with 10 ng/ml TNF-α for 24 hours. The ethyl acetate fraction of Marsilea cre

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7

Bozycki, Lukasz, Joanna Mroczek, Laurence Bessueille, et al. "Annexins A2, A6 and Fetuin-A Affect the Process of Mineralization in Vesicles Derived from Human Osteoblastic hFOB 1.19 and Osteosarcoma Saos-2 Cells." International Journal of Molecular Sciences 22, no. 8 (2021): 3993. http://dx.doi.org/10.3390/ijms22083993.

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The mineralization process is initiated by osteoblasts and chondrocytes during intramembranous and endochondral ossifications, respectively. Both types of cells release matrix vesicles (MVs), which accumulate Pi and Ca2+ and form apatites in their lumen. Tissue non-specific alkaline phosphatase (TNAP), a mineralization marker, is highly enriched in MVs, in which it removes inorganic pyrophosphate (PPi), an inhibitor of apatite formation. MVs then bud from the microvilli of mature osteoblasts or hypertrophic chondrocytes and, thanks to the action of the acto-myosin cortex, become released to th

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8

Islamov, Damir R., Vladimir N. Kruchinin, Vladimir Sh Aliev, et al. "Potential Fluctuation in RRAM Based on Non-Stoichiometric Hafnium Sub-Oxides." Advances in Science and Technology 99 (October 2016): 69–74. http://dx.doi.org/10.4028/www.scientific.net/ast.99.69.

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We study the structure of nonstoichiometric HfOx films with variable composition using methods of XPS, spectroscopic ellipsometry, and ab initio calculations. According to XPS and optical absorption experiment data HfOx consists of metal Hf and ~10-15% of nonstoichiometric hafnium sub-oxide HfOy (y<2). HfOy can be placed between HfO2 and Hf, inside HfO2, inside Hf. According to this model space fluctuations of chemical composition cause space fluctuations of bandgap in HfOx. We found that transport in such electronic systems is described by percolation theory. This approach can be applied t

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Karaca, Mehmet Ali, Derya Dilek Kancagi, Ugur Ozbek, Ercument Ovali, and Ozgul Gok. "Betulin Stimulates Osteogenic Differentiation of Human Osteoblasts-Loaded Alginate–Gelatin Microbeads." Bioengineering 11, no. 6 (2024): 553. http://dx.doi.org/10.3390/bioengineering11060553.

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Osteoporosis, a terminal illness, has emerged as a global public health problem in recent years. The long-term use of bone anabolic drugs to treat osteoporosis causes multi-morbidity in elderly patients. Alternative therapies, such as allogenic and autogenic tissue grafts, face important issues, such as a limited source of allogenic grafts and tissue rejection in autogenic grafts. However, stem cell therapy has been shown to increase bone regeneration and decrease osteoporotic bone formation. Stem cell therapy combined with betulin (BET) supplementation might be adequate for bone remodeling an

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Ma’arif, Burhan, Agnis Pondineka Ria Aditama, Faisal Akhmal Muslikh, et al. "Efek Penghambatan Ekspresi ER? Bebas oleh Fraksi n-Butanol Daun Semanggi (Marsilea crenata Presl.) pada Sel hFOB 1.19." Jurnal Sains dan Kesehatan 3, no. 4 (2021): 475–81. http://dx.doi.org/10.25026/jsk.v3i4.635.

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Wanita pascamenopause dapat menyebabkan berbagai macam masalah kesehatan, diantaranya adalah osteoporosis yang diakibatkan karena gangguan produksi hormon estrogen dalam tubuh. Salah satu alternatif yang muncul dan berpotensi untuk menggantikan hormon estrogen adalah fitoestrogen. Daun M. crenata diketahui mengandung senyawa fitoestrogen. Tujuan dari penelitian ini adalah untuk mengetahui fraksi n-Butanol daun M. crenata dalam meningkatkan aktivitas osteoblas pada proses pembentukan tulang terhadap sel hFOB 1.19. Penelitian ini menggunakan metode ICC dengan bantuan instrumen CLSM. Penelitian i

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11

Cui, Yan Chao, Yu Sheng Qiu, Qiong Wu, et al. "Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: A phenotypic microarray analysis." Experimental Biology and Medicine 246, no. 10 (2021): 1177–83. http://dx.doi.org/10.1177/1535370220985468.

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Osteoblasts play an important role in bone regeneration and repair. The hypoxia condition in bone occurs when bone undergoes fracture, and this will trigger a series of biochemical and mechanical changes to enable bone repair. Hence, it is interesting to observe the metabolites and metabolism changes when osteoblasts are exposed to hypoxic condition. This study has looked into the response of human osteoblast hFOB 1.19 under normoxic and hypoxic conditions by observing the cell growth and utilization of metabolites via Phenotype MicroArrays™ under these two different oxygen concentrations. The

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Aditama, Agnis Pondineka Ria, Siti Asiyah, Tintin Hariyani, Wahyu Nuraisya, Linda Andri Mustofa, and Anis Setyowati. "Enhancement of Osteoblastogenesis in the hFOB 1.19 Cell Line by the Induction of the n-Butanol Fraction Derived from Marsilea crenata C Presl. Leaves." Biomedical and Pharmacology Journal 17, no. 3 (2024): 1807–14. http://dx.doi.org/10.13005/bpj/2985.

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Osteoporosis is a disease characterized by bone loss, which can be treated by increasing the bone formation process. A marker of increased bone formation processes is the activity of the transcription factor Osx which stimulates the formation of Ocn. M. crenata leaves contains phytoestrogens which are thought to increase bone formation. The purpose of this research was to prove that the n-butanol fraction from the leaves of M. crenata can increase bone formation in hFOB 1.19 osteoblast cells by increasing the activity of the transcription factors Osx and Ocn. hFOB 1.19 cells that had reached c

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13

Rizzo, Maria Giovanna, Nicoletta Palermo, Paola Alibrandi, et al. "Physiologic Response Evaluation of Human Foetal Osteoblast Cells within Engineered 3D-Printed Polylactic Acid Scaffolds." Biology 12, no. 3 (2023): 424. http://dx.doi.org/10.3390/biology12030424.

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Large bone defect treatments have always been one of the important challenges in clinical practice and created a huge demand for more efficacious regenerative approaches. The bone tissue engineering (BTE) approach offered a new alternative to conventional bone grafts, addressing all clinical needs. Over the past years, BTE research is focused on the study and realisation of new biomaterials, including 3D-printed supports to improve mechanical, structural and biological properties. Among these, polylactic acid (PLA) scaffolds have been considered the most promising biomaterials due to their goo

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14

Hayes, Catriona A., Paul Dowling, Richard W. J. Groen, et al. "Cells of the Osteoblast Lineage Confer Myeloma Cell Resistance to Established and Investigational Therapeutic Agents." Blood 120, no. 21 (2012): 3995. http://dx.doi.org/10.1182/blood.v120.21.3995.3995.

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Abstract Abstract 3995 Introduction: Multiple myeloma (MM) cells suppress osteoblast (OB) maturation and function and induce osteoclast-mediated bone resorption. However, immature cells of the osteoblast lineage (e.g. pre-osteoblasts) remain present in the MM bone microenvironment and their impact on MM cell response to treatment has not been fully characterized. We therefore studied human immortalized cells of the osteoblast lineage as surrogate models for in vitro studies on the effects of pre-osteoblasts on MM cell proliferation, survival and drug resistance. Methods: Immortalized hFOB 1.19

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15

Carson, Matthew, Julia K. Keppler, Gilles Brackman, et al. "Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities." Cells Tissues Organs 206, no. 1-2 (2018): 106–18. http://dx.doi.org/10.1159/000494732.

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Polyphenols are known for their antimicrobial activity, whilst both polyphenols and the globular protein β-lactoglobulin (bLG) are suggested to have antioxidant properties and promote cell proliferation. These are potentially useful properties for a tissue-engineered construct, though it is unknown if they are retained when both compounds are used in combination. In this study, a range of different microbes and an osteoblast-like cell line (human fetal osteoblast, hFOB) were used to assess the combined effect of: (1) green tea extract (GTE), rich in the polyphenol epigallocatechin gallate (EGC

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16

Donahue, Henry J., Zhongyong Li, Zhiyi Zhou, and Clare E. Yellowley. "Differentiation of human fetal osteoblastic cells and gap junctional intercellular communication." American Journal of Physiology-Cell Physiology 278, no. 2 (2000): C315—C322. http://dx.doi.org/10.1152/ajpcell.2000.278.2.c315.

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Gap junctional channels facilitate intercellular communication and in doing so may contribute to cellular differentiation. To test this hypothesis, we examined gap junction expression and function in a temperature-sensitive human fetal osteoblastic cell line (hFOB 1.19) that when cultured at 37°C proliferates rapidly but when cultured at 39.5°C proliferates slowly and displays increased alkaline phosphatase activity and osteocalcin synthesis. We found that hFOB 1.19 cells express abundant connexin 43 (Cx43) protein and mRNA. In contrast, Cx45 mRNA was expressed to a lesser degree, and Cx26 and

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Shi, Jian, Lian Zhao, Brittany Duncan та ін. "Osteoblast-Induced Prostate Cancer Cell Migration and Invasion Is Mediated Through TGF-β1/SMAD2 Signal Pathway and Blocked by 17α-Estradiol". Journal of the Endocrine Society 5, Supplement_1 (2021): A1029. http://dx.doi.org/10.1210/jendso/bvab048.2105.

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Abstract Prostate cancer (PCa) is curable if it is diagnosed and treated in localized and regional stage. However, PCa outcome is poor once it has distant metastasis. Approximately 70% to 100% of PCa deaths have bone metastasis, which may be associated with a specific bone microenvironment. In this study, we investigated the effect and molecular mechanism of osteoblast cells on stimulation of PCa cell migration and invasion and examined the effectiveness of 17α-estradiol on blocking osteoblast-induced PCa cell migration and invasion using in vitro cell analysis. PCa cells (PC3, LNCaP and DU145

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18

Bach Knudsen, Knud Erik, Henry Jørgensen, and Nuria Canibe. "Quantification of the absorption of nutrients derived from carbohydrate assimilation: model experiment with catheterised pigs fed on wheat- or oat-based rolls." British Journal of Nutrition 84, no. 4 (2000): 449–58. http://dx.doi.org/10.1017/s0007114500001756.

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The main purpose of this study was to quantify the absorption of nutrients derived from carbohydrate assimilation in a model experiment with catheterised pigs. A low-fibre (LF) diet based on wheat flour and two high-fibre diets with added insoluble fibre from wheat bran (HFWB) or soluble fibre from oat bran (HFOB) were used. The diets were offered as baked rolls to three catheterised pigs in a 3×3 Latin square design. The pigs were surgically fitted with catheters placed in the portal vein and mesenteric artery and with an ultrasonic flow probe attached to the portal vein to monitor the blood-

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Hofbauer, Lorenz C., Francesca Gori, B. Lawrence Riggs, et al. "Stimulation of Osteoprotegerin Ligand and Inhibition of Osteoprotegerin Production by Glucocorticoids in Human Osteoblastic Lineage Cells: Potential Paracrine Mechanisms of Glucocorticoid-Induced Osteoporosis1." Endocrinology 140, no. 10 (1999): 4382–89. http://dx.doi.org/10.1210/endo.140.10.7034.

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Abstract Osteoporosis is a serious complication of systemic glucocorticoid use. However, while glucocorticoids increase bone resorption in vitro and in vivo, the mechanism(s) of this effect are at present unclear. Recent studies have identified the osteoprotegerin (OPG) ligand (OPG-L) as the final effector of osteoclastogenesis, an action that is opposed by the soluble neutralizing receptor, OPG. Thus, we assessed glucocorticoid regulation of OPG and OPG-L in various human osteoblastic lineage cells using Northern analysis, RT-PCR, and ELISA. Dexamethasone inhibited constitutive OPG messenger

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Ibrahim, Shirin, Syazana Abu Bakar, Mohamad Azmirruddin Ahmad, Nurul Awanis Johan, Siti Farhana Hisham, and K. Jamuna-Thevi. "Osteogenesis and Degradability of Bioresorbable Biphasic Gypsum-Carbonated Apatite Granules for Bone Tissue Engineering." Key Engineering Materials 705 (August 2016): 297–303. http://dx.doi.org/10.4028/www.scientific.net/kem.705.297.

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Osteogenesis and degradability of bioresorbable biphasic gypsum-carbonated apatite granules (BPG) were investigated. Three different sizes of gypsum, 300-600 μm (small), 600-1000 μm (medium) and 1000-2000 μm (large), denoted as S, M and L respectively, were developed through the crushing and sieving method. Exposure of gypsum granules in carbonate and phosphate sources formed BPG through dissolution and precipitation mechanism. BPG was firstly examined by X-ray Diffractometer (XRD) and Fourier Transform Infrared Spectrometer (FTIR) to confirm its phase and chemical composition respectively. In

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Pallavi, Manishi, Jenora Waterman, Youngmi Koo, Jagannathan Sankar, and Yeoheung Yun. "Assessment of Cytotoxicity of Magnesium Oxide and Magnesium Hydroxide Nanoparticles using the Electric Cell-Substrate Impedance Sensing." Applied Sciences 10, no. 6 (2020): 2114. http://dx.doi.org/10.3390/app10062114.

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Magnesium (Mg)-based alloys have the potential for bone repair due to their properties of biodegradation, biocompatibility, and structural stability, which can eliminate the requirement for a second surgery for the removal of the implant. Nevertheless, uncontrolled degradation rate and possible cytotoxicity of the corrosion products at the implant sites are known current challenges for clinical applications. In this study, we assessed in vitro cytotoxicity of different concentrations (0 to 50 mM) of possible corrosion products in the form of magnesium oxide (MgO) and magnesium hydroxide (Mg(OH

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Nizan, Nor Sarahtul Nadirah Hairol, Farah Hanani Zulkifli, Hazrulrizawati Abd Hamid, and Muhammad Hafiz Mazwir. "Effect of Different Concentration of Cellulose Nanocrystals Comprising Hydroxyethyl Cellulose / Poly(Vinyl Alcohol) as a Bone Tissue Engineering Scaffold." Materials Science Forum 981 (March 2020): 285–90. http://dx.doi.org/10.4028/www.scientific.net/msf.981.285.

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In this study, biodegradable scaffolds based on hydroxyethyl cellulose (HEC) (5 wt%) and poly (vinyl alcohol) (PVA) (15 wt%) with different percentages of celullose nanocrystal (CNC) (1 and 7 wt%) were fabricated by lyophilization method to get highly porous scaffolds. These scaffolds were made water insoluble by cross-linking via heat treatment. The morphology and thermal properties of HEC/PVA/CNCs scaffolds were characterized by using Scanning Electron Microscope (SEM) and Thermogravimetric Analysis (TGA). The morphological study showed that both prepared scaffold have highly porous structur

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Mann, Vivek, Daniela Grimm, Thomas Corydon, et al. "Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering." International Journal of Molecular Sciences 20, no. 6 (2019): 1357. http://dx.doi.org/10.3390/ijms20061357.

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Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (RPM) for 7 days and 14 days, with the purpose of investigating the effects of s-µg on biological processes and to engineer 3D bone constructs. RPM exposure of the hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, extra cellular matrix (ECM) and the 3D multicellular spheroid (MC

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Stoilov, Milan, Lea Stoilov, Norbert Enkling, et al. "Effects of Different Titanium Surface Treatments on Adhesion, Proliferation and Differentiation of Bone Cells: An In Vitro Study." Journal of Functional Biomaterials 13, no. 3 (2022): 143. http://dx.doi.org/10.3390/jfb13030143.

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The objective of this study was to evaluate the impacts of different sandblasting procedures in acid etching of Ti6Al4V surfaces on osteoblast cell behavior, regarding various physicochemical and topographical parameters. Furthermore, differences in osteoblast cell behavior between cpTi and Ti6Al4V SA surfaces were evaluated. Sandblasting and subsequent acid etching of cpTi and Ti6Al4V discs was performed with Al2O3 grains of different sizes and with varying blasting pressures. The micro- and nano-roughness of the experimental SA surfaces were analyzed via confocal, atomic force and scanning e

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Pallavi, Manishi, Jenora Waterman, Youngmi Koo, Jagannathan Sankar, and Yeoheung Yun. "In Vitro Cytotoxicity of Possible Corrosion Products from Mg-Based Biodegradable Metals: Magnesium Oxide and Magnesium Hydroxide Nanoparticles." Applied Sciences 9, no. 20 (2019): 4304. http://dx.doi.org/10.3390/app9204304.

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Biodegradable magnesium (Mg) alloys have potential applications in orthopedic implants due to their mechanical and osseointegration properties. However, the surface characteristics, biocompatibility, and toxicity of the released corrosion products in the form of magnesium oxide (MgO) and magnesium hydroxide (Mg(OH)2) nanoparticles (NPs) at the junction of implants and in the surrounding tissue are not completely understood. Here, we investigated in vitro cytotoxicity and morphological changes in human fetal osteoblast (hFOB) 1.19 cells in response to various concentrations (1 mM, 5 mM, 10 mM,

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Yugang, Wang, Fan Qiming, Ma Rui, Lin Wentao, and Tang Tingting. "Gene expression profiles and phosphorylation patterns of AMP-activated protein kinase subunits in various mesenchymal cell types." Chinese Medical Journal 127, no. 13 (2014): 2451–57. http://dx.doi.org/10.3760/cma.j.issn.0366-6999.20131882.

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Background Recent studies on bone have shown an endocrine role of the skeleton, which could be impaired in various human diseases, including osteoporosis, obesity, and diabetes-associated bone diseases. As a sensor and regulator of energy metabolism, AMP-activated protein kinase (AMPK) may also play an important role in the regulation of bone metabolism. The current study aimed to establish the expression profiles and phosphorylation patterns of AMPK subunits in several mesenchymal cell types. Methods Reverse transcription-polymerase chain reaction (PCR) for relative quantification, real-time

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Grzeczkowicz, Anna, Monika Drabik, Agata Lipko, et al. "A Composite Membrane System with Gold Nanoparticles, Hydroxyapatite, and Fullerenol for Dual Interaction for Biomedical Purposes." Membranes 11, no. 8 (2021): 565. http://dx.doi.org/10.3390/membranes11080565.

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Background: Wound dressing plays a vital role in post-operative aftercare. There is the necessity to develop dressings for application on the border of soft and hard tissue. This study aimed to develop multifunctional polyelectrolyte layers enhanced by hydroxyapatite nanoparticles, gold nanoparticles (AuNPs), and/or fullerenol nanocomposites to achieve a wound dressing that could be applied on the bone-skin interface. Methods: Constructed shells were examined using TEM, STEM, and EDX techniques. The human osteoblasts or fibroblasts were immobilized within the shells. The systems morphology was

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Mohd Fozi, Nur Farhana, James Jam Jolly, Kien Hui Chua, Ekram Alias, Kok Yong Chin, and Ima Nirwana Soelaiman. "Comparing the Effects of Alpha-Tocopherol and Tocotrienol Isomers on Osteoblasts hFOB 1.19 Cultured on Bovine Bone Scaffold." Sains Malaysiana 50, no. 8 (2021): 2319–28. http://dx.doi.org/10.17576/jsm-2021-5008-15.

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Tocotrienol mixtures have been shown to exert anabolic actions on the skeletal system in animal studies, but it is unclear which tocotrienol isomer shows the most prominent effects. This study aims to investigate the most active tocotrienol isomers using hFOB 1.19 human osteoblasts cultured on a bovine bone scaffold. The bovine trabecular bone was sectioned, demineralised and freeze-dried to form the scaffold. hFOB 1.19 osteoblasts were cultured on the bone scaffolds in humidified condition at 37 °C and 5% carbon dioxide with vitamin E isomers (alpha-, beta-, gamma-, delta-tocotrienol and alph

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Ueland, Thor, Tove Lekva, Kari Otterdal та ін. "Increased serum and bone matrix levels of transforming growth factor β1 in patients with GH deficiency in response to GH treatment". European Journal of Endocrinology 165, № 3 (2011): 393–400. http://dx.doi.org/10.1530/eje-11-0442.

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ObjectivePatients with adult onset GH deficiency (aoGHD) have secondary osteoporosis, which is reversed by long-term GH substitution. Transforming growth factor β1 (TGFβ1 or TGFB1) is abundant in bone tissue and could mediate some effects of GH/IGFs on bone. We investigated its regulation by GH/IGF1in vivoandin vitro.Design and methodsThe effects of GH substitution (9–12 months, placebo controlled) on circulating and cortical bone matrix contents of TGFβ1 were investigated in patients with aoGHD. The effects of GH/IGF1 on TGFβ1 secretion in osteoblasts (hFOB), adipocytes, and THP-1 macrophages

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Behboodi, Panteha, Angelica Castano, Iriana Colorado, et al. "Abstract 5237: Optimizing multiple myeloma treatment approaches by elucidating tumor-stroma interactions in the bone marrow microenvironment." Cancer Research 85, no. 8_Supplement_1 (2025): 5237. https://doi.org/10.1158/1538-7445.am2025-5237.

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Multiple Myeloma (MM) is a plasma cell malignancy arising in the bone marrow. Despite advancements in therapies, this disease becomes refractory such that relapse and death is inevitable. Immune evasion and drug resistance are partly due to MM-osteoblast cell interactions in the bone marrow microenvironment (BME). We conducted complementary in-vitro and in-vivo studies, each providing insights that converge to a treatment-relevant hypothesis. Several human MM cell lines (MM.1S, U266, NCI-H929, L363) were co-cultured with osteoblasts for two days to assess proliferation in response to two prote

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Szustakiewicz, Konrad, Marcin Włodarczyk, Małgorzata Gazińska, et al. "The Effect of Pore Size Distribution and l-Lysine Modified Apatite Whiskers (HAP) on Osteoblasts Response in PLLA/HAP Foam Scaffolds Obtained in the Thermally Induced Phase Separation Process." International Journal of Molecular Sciences 22, no. 7 (2021): 3607. http://dx.doi.org/10.3390/ijms22073607.

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In this research, we prepared foam scaffolds based on poly(l-lactide) (PLLA) and apatite whiskers (HAP) using thermally induced phase separation technique supported by the salt leaching process (TIPS-SL). Using sodium chloride having a size of (a) 150–315 μm, (b) 315–400 μm, and (c) 500–600 μm, three types of foams with different pore sizes have been obtained. Internal structure of the obtained materials has been investigated using SEM as well as μCT. The materials have been studied by means of porosity, density, and compression tests. As the most promising, the composite prepared with salt si

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Aditama, Agnis Pondineka Ria, Dewi Taurisiawati Rahayu, Brivian Florentis Yustanta, et al. "The Effect of Water Fraction Derived from Green Clover (Marsilea crenata C. Presl.) Leaves on Differentiation and Maturation of Human Osteoblast Cell." Biomedical and Pharmacology Journal 17, no. 3 (2024): 1749–55. http://dx.doi.org/10.13005/bpj/2980.

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Women over the age of 40 often experience a decrease in bone mass due to a lack of estrogen, which can increase the risk of osteoporosis. Previous studies have shown that the leaves of Green Clover (Marsilea crenata C. Presl.) contains phytoestrogen compounds which can potentially replace the function of estrogen in the body. In this context, this study aims to evaluate the ability of water fractions from M. crenata leaves in increasing the expression of osterix (Osx) and osteocalcin (Ocn), which are important indicators of increased bone formation, specificially on differentiation and maturat

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Gaitán-Salvatella, Iñigo, Patricia González-Alva, Juan José Montesinos, and Marco Antonio Alvarez-Perez. "In Vitro Bone Differentiation of 3D Microsphere from Dental Pulp-Mesenchymal Stem Cells." Bioengineering 10, no. 5 (2023): 571. http://dx.doi.org/10.3390/bioengineering10050571.

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Bone defects lead to the structural loss of normal architecture, and those in the field of bone tissue engineering are searching for new alternatives to aid bone regeneration. Dental pulp-mesenchymal stem cells (DP-MSC) could provide a promising alternative to repair bone defects, principally due to their multipotency and capacity to fabricate three-dimensional (3D) spheroids. The present study aimed to characterize the 3D DP-MSC microsphere and the osteogenic differentiation capacity potential cultured by a magnetic levitation system. To achieve this, the 3D DP-MSC microsphere was grown for 7

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Franco, Domenico, Antonio Alessio Leonardi, Maria Giovanna Rizzo, et al. "Biological Response Evaluation of Human Fetal Osteoblast Cells and Bacterial Cells on Fractal Silver Dendrites for Bone Tissue Engineering." Nanomaterials 13, no. 6 (2023): 1107. http://dx.doi.org/10.3390/nano13061107.

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Prosthetic joint replacement is the most widely used surgical approach to repair large bone defects, although it is often associated with prosthetic joint infection (PJI), caused by biofilm formation. To solve the PJI problem, various approaches have been proposed, including the coating of implantable devices with nanomaterials that exhibit antibacterial activity. Among these, silver nanoparticles (AgNPs) are the most used for biomedical applications, even though their use has been limited by their cytotoxicity. Therefore, several studies have been performed to evaluate the most appropriate Ag

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Mizerska-Kowalska, Magdalena, Adrianna Sławińska-Brych, Katarzyna Kaławaj, et al. "Betulin Promotes Differentiation of Human Osteoblasts In Vitro and Exerts an Osteoinductive Effect on the hFOB 1.19 Cell Line Through Activation of JNK, ERK1/2, and mTOR Kinases." Molecules 24, no. 14 (2019): 2637. http://dx.doi.org/10.3390/molecules24142637.

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Although betulin (BET), a naturally occurring pentacyclic triterpene, has a variety of biological activities, its osteogenic potential has not been investigated so far. The aim of this study was to assess the effect of BET on differentiation of human osteoblasts (hFOB 1.19 and Saos-2 cells) in vitro in osteogenic (with ascorbic acid as an osteogenic supplement) and osteoinductive (without an additional osteogenic supplement) conditions. Osteoblast differentiation was evaluated based on the mRNA expression (RT-qPCR) of Runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), typ

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Aditama, Agnis P. R., Burhan Ma’arif, Hening Laswati, and Mangestuti Agil. "In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent." Journal of Basic and Clinical Physiology and Pharmacology 32, no. 4 (2021): 881–87. http://dx.doi.org/10.1515/jbcpp-2020-0515.

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Abstract Objectives Osteoporosis is the result of an imbalance in the rate of bone resorption and bone formation due to a decrease in estrogen. Phytoestrogens are plant compounds with structures and functions similar to estrogen. Phytoestrogens that bind to estrogen receptors in bone cells are able to modulate bone formation. Semanggi (Marsilea crenata Presl.) is a plant that contains phytoestrogens. The purpose of this study was to observe the expression of osteocalcin and predict the content of extract phytoestrogens through a computer simulation study to study the bone formation activity of

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Chen, Guangnan, Tingting Fang, Zhongming Huang, et al. "MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R." Cellular Physiology and Biochemistry 38, no. 2 (2016): 598–608. http://dx.doi.org/10.1159/000438653.

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Background/Aims: MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression by repressing translation or cleaving RNA transcripts in a sequence-specific manner. Downregulated microRNAs and their roles in cancer development have attracted much attention. A growing body of evidence showed that microRNA-133a (miR-133a) has inhibitory effects on cell proliferation, migration, invasion, and metastasis of osteosarcoma. Methods: MiR-133a expression in human osteosarcoma cell lines and human normal osteoblastic cell line hFOB was investigated by real-time PCR (RT-PCR). The ro

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Predoi, Daniela, Simona Liliana Iconaru, Carmen Steluta Ciobanu, et al. "Studies of the Tarragon Essential Oil Effects on the Characteristics of Doped Hydroxyapatite/Chitosan Biocomposites." Polymers 15, no. 8 (2023): 1908. http://dx.doi.org/10.3390/polym15081908.

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Due to the emergence of antibiotic-resistant pathogens, the need to find new, efficient antimicrobial agents is rapidly increasing. Therefore, in this study, we report the development of new biocomposites based on zinc-doped hydroxyapatite/chitosan enriched with essential oil of Artemisia dracunculus L. with good antimicrobial activity. Techniques such as scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX) and Fourier transform infrared spectroscopy (FTIR) were used in order to evaluate their physico-chemical properties. Our studies revealed

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Safitri, Putri Gita Ayu, Muhammad Da’i, and Febri Wulandari. "1’- Acetoxychavicol acetate Suppresses Osteosarcoma Cell Proliferation through the PI3K Pathway: A Molecular Docking and Cytotoxicity Study." Molecular and Cellular Biomedical Sciences 9, no. 2 (2025): 105. https://doi.org/10.21705/mcbs.v9i2.640.

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Background: This study aims to investigate anticancer properties of 1'-Acetoxychavicol acetate (ACA), a phenylpropanoid substance obtained from the rhizomes of the Alpinia genus, which has been extensively studied. However research on its cytotoxic effects, particularly against osteosarcoma cells, has never been donenot been conducted. The purpose of the research is to investigate the anticancer potential of ACA to support the its development as a novel therapeutic candidate.Materials and methods: This study assessed ACA’s initial anticancer potential through in vitro cytotoxic tests on normal

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Subramaniam, M., Syed M. Jalal, David J. Rickard, Steven A. Harris, Mark E. Bolander, and Thomas C. Spelsberg. "Further characterization of human fetal osteoblastic hFOB 1.19 and hFOB/ER? cells: Bone formation in vivo and karyotype analysis using multicolor fluorescent in situ hybridization." Journal of Cellular Biochemistry 87, no. 1 (2002): 9–15. http://dx.doi.org/10.1002/jcb.10259.

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Zhu, Hongbin, Zongbao Gao, Tao Wang, Zhigang Lei, and Maoqin Sun. "Effect of LncRNA BLACAT1 on the Proliferation and Apoptosis of Osteoblasts in Ankylosing Spondylitis Through Regulating RANKL/OPG Signaling Pathway." Journal of Biomaterials and Tissue Engineering 9, no. 9 (2019): 1279–85. http://dx.doi.org/10.1166/jbt.2019.2132.

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Ankylosing spondylitis (AS) is an autoimmune disorder. LncRNA BLACAT1 involves in several diseases such as inflammation and immune diseases, but the expression and role of LncRNA BLACAT1 in AS remains unclear. AS patients and controls were selected. LcRNA BLACAT1 expression in peripheral blood was analyzed by Real time PCR, and its correlation with CRP, ESR and AS activity was analyzed. Osteoblast hFOB 1.19 was cultured and transfected with LncRNA BLACAT1 plasmid and si-LncRNA BLACAT1, respectively followed by analysis of cell proliferation by MTT assay, apoptosis by Caspase 3, ADAMTS-4 and Ru

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Heffernan, Shane M., Mark Waldron, Kirsty Meldrum, Stephen J. Evans, and Gillian E. Conway. "Red Algae Alters Expression of Inflammatory Pathways in an Osteoarthritis In Vitro Co-Culture." Pharmaceuticals 18, no. 3 (2025): 315. https://doi.org/10.3390/ph18030315.

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Background/Objectives: Osteoarthritis (OA) is one of the most prevalent chronic conditions and significantly contributes to local and global disease burden. Common pharmaceuticals that are used to treat OA cause significant side effects, thus non-pharmaceutical bioactive alternatives have been developed that can impact OA symptoms without severe side-effects. One such alternative is the Red Algae Lithothamnion species (Litho). However, there is little mechanistic knowledge of its potential to effect OA gene expression, and a human in vitro model using commercially available cell lines to test

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Xue, Yan, Hongmiao Liu, Guangchen Nie, and Xiaoping Ren. "lncRNA DARS-AS1 Promoted Osteosarcoma Progression through Regulating miR-532-3p/CCR7." Disease Markers 2022 (April 5, 2022): 1–9. http://dx.doi.org/10.1155/2022/4660217.

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Background. lncRNAs have been indicated to involve in cell invasion, proliferation, and metastasis. However, function of DARS-AS1 in osteosarcoma remains poorly explored. Methods. DARS-AS1 and miR-532-3p level were measured using qRT-PCR. CCK-8 assay and cell invasion assay were done to study cell functions. Luciferase reporter assay was performed to study the mechanism about DARS-AS1 and miR-532-3p. Results. We firstly showed that DARS-AS1 expression is upregulated in 73.5% (25/34) of cases with osteosarcoma. Moreover, DARS-AS1 expression is overexpressed in osteosarcoma specimens than in non

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Khoo, Lee Kian, Sirichai Kiattavorncharoen, Verasak Pairuchvej, Nisanat Lakkhanachatpan, Natthamet Wongsirichat, and Dutmanee Seriwatanachai. "The Affinity of Human Fetal Osteoblast to Laser-Modified Titanium Implant Fixtures." Open Dentistry Journal 14, no. 1 (2020): 52–58. http://dx.doi.org/10.2174/1874210602014010052.

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Introduction: Implant surface modification methods have recently involved laser treatment to achieve the desired implant surface characteristics. Meanwhile, surface modification could potentially introduce foreign elements to the implant surface during the manufacturing process. Objectives: The study aimed to investigate the surface chemistry and topography of commercially available laser-modified titanium implants, together with evaluating the cell morphology and cell adhesion of human fetal osteoblast (hFOB) seeded onto the same implants. Method: Six (6) samples of commercially available las

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Zo, Sunmi, Soonmo Choi, Hyunduk Kim, Eunjoo Shin, and Sungsoo Han. "Synthesis and Characterization of Carboxymethyl Chitosan Scaffolds Grafted with Waterborne Polyurethane." Journal of Nanoscience and Nanotechnology 20, no. 8 (2020): 5014–18. http://dx.doi.org/10.1166/jnn.2020.17844.

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Bone tissue engineering has been rapidly developed in regenerative medicine field, which aims to induce new functional bone regeneration through the synergistic combination of biomaterials and cells. Porous biomaterials with sufficient mechanical properties and functional impregnating for bone substitutes have been imposed in the oncoming generation of bone reconstruction. In this study, we fabricated Carboxymethyl chitosan three dimensional (3D) porous scaffold modified with waterborne polyurethane (WPU) through freeze drying technique. In order to check its potential in bone tissue substitut

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Guo, Donggeng, Qiuxia Li, Qing Lv, Qiujing Wei, Shuangyan Cao, and Jieruo Gu. "MiR-27a Targets sFRP1 in hFOB Cells to Regulate Proliferation, Apoptosis and Differentiation." PLoS ONE 9, no. 3 (2014): e91354. http://dx.doi.org/10.1371/journal.pone.0091354.

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Kim, Minah, Minji Choi, Yong-Dae Kwon, Joo-Young Ohe, and Junho Jung. "The Potential of Enamel Matrix Derivative in Countering Bisphosphonate-Induced Effects in Osteoblasts." Life 14, no. 9 (2024): 1088. http://dx.doi.org/10.3390/life14091088.

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The suppressive effect of bisphosphonates (BPs) on bone metabolism is considered to be a major cause of medication-related osteonecrosis of the jaw (MRONJ). Enamel matrix derivative (EMD) stimulates and activates growth factors, leading to the regeneration of periodontal tissues. In this study, we aimed to explore the potential of EMD in reversing the detrimental effects of BPs on human fetal osteoblasts (hFOBs) and osteosarcoma-derived immature osteoblasts (MG63s) by assessing cell viability, apoptosis, migration, gene expression, and protein synthesis. While the suppressive effect of zoledro

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Vasilescu, Vlad Gabriel, Toma Lucian Ciocan, Andreea Mihaela Custura, et al. "Biocompatible and Antibacterial Chemical Coatings on TiZr Dental Implants." Journal of Functional Biomaterials 16, no. 3 (2025): 112. https://doi.org/10.3390/jfb16030112.

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This research aims to study the antibacterial coatings of invasive surgical medical devices, including dental implants, to reduce superficial and deep local infections over the long term. To obtain the coating without altering the initial properties of the substrate (dental implant made of TiZr bioalloy), simple, cost-effective, and efficient methods were employed, such as chemical deposition of silver (Ag). The deposition characteristics were analyzed using scanning electron microscopy (SEM), EDX analysis, and FT-IR infrared analysis. The in vitro testing of antimicrobial activity was conduct

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Li, Wen-Tyng, John W. Shriver, and John N. Reeve. "Mutational Analysis of Differences in Thermostability between Histones from Mesophilic and Hyperthermophilic Archaea." Journal of Bacteriology 182, no. 3 (2000): 812–17. http://dx.doi.org/10.1128/jb.182.3.812-817.2000.

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ABSTRACT Amino acid residues responsible for the large difference in thermostability between HMfB and HFoB, archaeal histones from the hyperthermophile Methanothermus fervidus and the mesophileMethanobacterium formicicum, respectively, have been identified by site-specific mutagenesis. The thermal denaturation of ∼70 archaeal histone variants has been monitored by circular dichroism, and the data generated were fit to a two-state unfolding model (dimer→two random coil monomers) to obtain a standard-state (1M) melting temperature for each variant dimer. The results of single-, double-, and trip

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Babuska, Vaclav, Jana Dobra, Vlastimil Kulda, et al. "Comparison of Fibroblast and Osteoblast Response to Cultivation on Titanium Implants with Different Grain Sizes." Journal of Nanomaterials 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/920893.

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Thein vitroresponse of human fibroblast cell line HFL1 and human osteoblast cell line hFOB 1.19 on nanostructured titanium with different grain sizes has been compared in the present study. Used samples of titanium produced by equal channel angular (ECA) pressing have grain sizes of 160 nm, 280 nm, and 2400 nm with cross- and longitudinal sections. Similar cellular behaviour was observed on all studied biomaterials. There were significant differences related to the initial phase of attachment, but not in proliferation. Furthermore, the results indicate that osteoblasts grow best on material wi

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