Academic literature on the topic 'Hibiscus extract'
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Journal articles on the topic "Hibiscus extract"
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Putra, Imam Budi, Nelva K. Jusuf, and Imam Bagus Sumantri. "The Potency of Hibiscus rosa-sinensis Linn. Leaves Ethanol Extract as Hair Growth." Open Access Macedonian Journal of Medical Sciences 8, A (February 15, 2020): 89–92. http://dx.doi.org/10.3889/oamjms.2020.4211.
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INTRODUCTION: Hair is one of the organs and crowns for all people. Hair loss will really bother for most people. One of the plants that can be used as hair growth is Hibiscus rosa-sinensis Linn.
OBJECTIVE: The objective of the study was to find the potency of H. rosa-sinensis leaves ethanol extracts as hair growth.
METHODS: In this research, simplicia and extracts were made from hibiscus leaves using ethanol as a solvent. Simplicia and extracts were tested for phytochemical screening and characterization of each. The extract obtained was tested in vivo with animals test by observing mouse’s hair growth for 25 days of observation.
RESULTS: Simplicia and ethanol extract of Hibiscus leaves contain flavonoids, alkaloids, glycosides, tannins, and steroids/terpenoids. The characteristics of Hibiscus leaves simplicia and extract had water content (9.71% vs. 10.65%), water-soluble extract content (19.91% vs. 27.58%), ethanol-soluble extract content (37.86% vs. 49.51%), total ash content (7.96% vs. 9.46%), and acid-insoluble ash content (0.78% vs. 1.28%). In vivo testing with animals, ethanol extract of Hibiscus leaves had an effect as a hair growth until the 25th day with an average concentration of 2.5% (14 mm), 5% (16 mm), and 10% (19 mm).
DISCUSSION: The extract of Hibiscus leaves that showed the presence of carbohydrate, alkaloid, flavonoid, steroids, protein, tannin, and amino acids. The presence of active constituents such as flavonoids and tannins may be responsible for hair growth activity.
CONCLUSION: The ethanol extracts of H. rosa-sinensis leaves can increase hair growth, at optimum concentrations of 10% extract.
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Márquez-Rodríguez, Ana Selene, Susana Nevárez-Baca, Julio César Lerma-Hernández, León Raul Hernández-Ochoa, Guadalupe Virginia Nevárez-Moorillon, Néstor Gutiérrez-Méndez, Laila Nayzzel Muñoz-Castellanos, and Erika Salas. "In Vitro Antibacterial Activity of Hibiscus sabdariffa L. Phenolic Extract and Its In Situ Application on Shelf-Life of Beef Meat." Foods 9, no. 8 (August 8, 2020): 1080. http://dx.doi.org/10.3390/foods9081080.
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Compounds from spices and herbs extracts are being explored as natural antibacterial additives. A plant extract used in traditional folk medicine is Hibiscus sabdariffa L., also known as Roselle. Therefore, the potential use of a phenolic hibiscus extract as antibacterial or natural food preservative was analyzed in vitro and in situ. A phenolic extract was obtained from hibiscus calyces and fractionated, and then the fractions were tested against foodborne pathogen bacteria. Liquid–liquid extraction and solid-phase extraction were used to fractionate the hibiscus extract, and HPLC was employed to analyze the fractions’ phenolic composition. Minimum bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) were calculated for brute hibiscus phenolic extract, each of the fractions and pure commercial phenolic compounds. Bacteria tested were Escherichia coli, Salmonella enterica serovar Typhimurium, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. The fraction obtained after liquid–liquid extraction presented the best performance of MBC and MIC against the bacteria tested. Furthermore, a hibiscus ethanolic extract was employed as a natural preservative to extend the shelf-life of beef. Microbiological, color and sensory analyses were performed to the meat during the shelf-life test. The application of the phenolic hibiscus extract also showed an increase of the duration of the meat`s shelf life.
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C, Nwokonkwo Dorothy. "UTILIZATION OF AQUEOUS, METHANOL AND CHLOROFORM EXTRACTS OF LOCAL PLANTS- IXORA COCCINEA AND HIBISCUS SABDARRIFFA (ZOBO) FROM ABAKALIKI AS FABRIC DYES." JOURNAL OF ADVANCES IN CHEMISTRY 11, no. 5 (April 18, 2015): 3576–82. http://dx.doi.org/10.24297/jac.v11i5.4476.
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This investigation dealt with the dyeing and fastness properties of crude constituents of Ixora coccinea and Hibiscus sabdarriffa plant species extracted using distilled water, methanol and chloroform. Approximately 150 g of each sample was soaked in the chosen solvent for four weeks, the percentage yields of the aqueous, methanol and chloroform extracts of Ixora coccinea were 38.47% and 13.40 % and 3.88 % respectively; Hibiscus sabdariffa gave yields of 22.85 % for the aqueous extract, 12.24 % for the methanol extract and 3.79 % for the chloroform extract. Aqueous, methanol and chloroform extracts of Ixora coccinea were all pink in colour; Hibiscus sabdariffa gave red aqueous extract, red methanol extract and orange chloroform extract. The extracts were used without further purification in dyeing unmordanted and mordanted cotton and polyester fabrics. Different colour shades were obtained after dyeing. The mordanted fabrics using CuSO4, FeSO4 and K2CrO7 were fast to acid, alkali and washing.Key words dyeing, extract, fastness, mordant, solvent
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Tomar, Oktay. "The effect of plant extracts on antioxidant potential, microbial and sensory attributes of stirred yoghurt." Mljekarstvo 71, no. 1 (December 14, 2020): 35–48. http://dx.doi.org/10.15567/mljekarstvo.2021.0104.
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The aim of this study was to use various plants to increase the functional properties of yoghurt. The ethanol extracts of three different plant (Mentha piperita L., Ocimum basilicum L., and Hibiscus sabdariffa L.) extracts were added to the stirred type yoghurts at different ratios (0.1 %, 0.3 % and 0.5 %). The pH values, colour values, antioxidant activity, microbial and sensory attributes of yoghurt were evaluated on the storage days of 0, 7, 14, 21, and 28. The lowest pH values were found at the beginning (4.29) and at the end (3.95) of the storage period in samples containing 0.5 % hibiscus extract. The addition of plant extracts in amounts of 0.3 % and higher decreased the lightness (L*) value of yoghurt (p<0.05), and the maximum decrease was found in yoghurt samples containing 0.5% hibiscus ethanol extract. The concentrations of added mint and basil extracts increased the yellowness (b*) value (p<0.05), while the addition of hibiscus extract significantly increased the redness value (p<0.05). Hibiscus ethanol extract significantly reduced the growth of total aerobic mesophilic bacteria (TAMB), lactic acid bacteria (LAB) and Lactococcus/Streptococcus bacteria (p<0.05). The antioxidant activity of all samples increased with adding plant extracts. According to the results of sensory analysis, the addition of 0.1 % plant extracts to yoghurts rated higher scores than that of the control sample, but the addition of 0.3 % and 0.5 % ethanol extracts negatively affected the sensory properties. It can be concluded that adding plant extracts had a positive effect on the sensory and functional properties of yoghurt.
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Baranova, V. S., I. F. Rusina, D. A. Guseva, N. N. Prozorovskaya, O. M. Ipatova, and O. T. Kasaikina. "The antiradical activity of plant extracts and healthful preventive combinations of these exrtacts with the phospholipid complex." Biomeditsinskaya Khimiya 58, no. 6 (2012): 712–26. http://dx.doi.org/10.18097/pbmc20125806712.
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Using the chemiluminescence method, the effective concentration of antioxidants (AO) and its reactivity toward peroxyl radicals (ARA, the k7 constant) have been measured for 13 plant extracts. In fact all extracts demonstrated ARA higher than ionol. Larix dahurica, Hypericum perforatum, Potentilla fruticosa, Aronia melanocarpa and Rhaponticum carthamoides extracts showed the highest values of ARA. The combinations Aronia + Raponticum extracts; Larix + Hibiscus extracts; Schizandra +Aronia extracts were synergistic (the synergism effect β of 38%, 33% and 22%). Apparently this phenomenon is the result of the synergistic interaction between compounds present in plant extracts. The Phospholipid complex - Lipoid S40, lacting any antioxidant effect alone, showed a potent synergistic effect with Aronia extract (β = 60%), Silybum extract (β = 41%). Clinical trials demonstrated, that combinations "Lipoid + Aronia extract", "Lipoid + Larix extract + Hibiscus extract", "Lipoid + Silybum extract", "Lipoid + Q10 + Rosa majalis extract" may be used as an additional component in the medicinal treatment, or as an individual prophylactic agent.
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Truong, Han, Ly Le, and Minh Tran. "Phytochemical Screening and Total Phenolics and Flavonoids Contents of Hibiscus Rosa Sinensis. L Cultivated in Viet Nam." MedPharmRes 1, no. 1 (December 10, 2017): 52–57. http://dx.doi.org/10.32895/ump.mpr.1.1.52/suffix.
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Background: Phenolics and flavonoids are regarded as the highest potential of chemotherapeutic activities. This investigation was carried out to evaluate phytochemical and total phenolics content (TPC) and total flavonoids content (TFC) and Fourier-transform infrared spectroscopy (FTIR) spectral analysis of Hibiscus Rosa Sinensis L. extracts (crude extract, n-hexane extract, ethyl acetate extract, and methanol extract) for further applications in pharmaceutical development. Method: Total phenolics were estimated by Folin-Ciocalteu method; while, Aluminum chloride was employed to quantify total flavonoids in the sample extracts. And, functional groups of Hibiscus Rosa Sinensis compound was determined using a FTIR-spectrophotometer. Result: Results showed the leaves extracts to encompass the high amount of total phenolic and total flavonoid content. TPC values for crude extract, methanol extract, ethyl acetate extract and n-hexane extract were 57.09 ± 0.35 mg/g, 70.98 ± 0.03 mg/g, 21.31 ± 0.01 mg/g, and 18.45 ± 0.003 mg/g as gallic acid equivalent, respectively. Crude extract, methanol extract, ethyl acetate extract and n-hexane extract showed total flavonoids 26.87 ± 0.01 mg/g, 21.08 ± 0.03 mg/g, 21.70 ± 0.001 mg/g, 14.95 ± 0.02 mg/g as rutin equivalent. FTIR spectra of four extracts were comparable and showed the presence of nitro compounds and ring aromatic compounds. Conclusion: Our results indicate the potential of exploiting Hibiscus Rosa Sinensis leaves as a source of chemotherapeutic compounds, and it is worthy doing further researches on isolated bioactive compounds for developing novel functional foods or new drugs.
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Al-Yousef, Hanan M., Wafaa H. B. Hassan, Sahar Abdelaziz, Musarat Amina, Rasha Adel, and May A. El-Sayed. "UPLC-ESI-MS/MS Profile and Antioxidant, Cytotoxic, Antidiabetic, and Antiobesity Activities of the Aqueous Extracts of Three Different Hibiscus Species." Journal of Chemistry 2020 (June 25, 2020): 1–17. http://dx.doi.org/10.1155/2020/6749176.
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The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50 = 50 ± 5.1 μg/mL, and Hibiscus micranthus showed a close effect with IC50 = 60.4 ± 1.7 μg/mL. Hibiscus micranthus showed the most potent effect on HCT-116 (human colon carcinoma) with IC50 = 56 ± 1.9 μg/mL compared with cisplatin (IC50 = 7.53 ± 3.8 μg/mL). HcA and HdA extracts showed weak cytotoxic activity against A-549 and HCT-116 cell lines compared to the other extracts. Eventually, Hibiscus deflersii showed astonishing antidiabetic (IC50 = 56 ± 1.9 μg/mL) and antiobesity (IC50 = 95.45 ± 1.9 μg/mL) activities using in vitro α-amylase inhibitory assay (compared with acarbose (IC50 = 34.71 ± 0.7 μg/mL)) and pancreatic lipase inhibitory assay (compared with orlistat (IC50 = 23.8 ± 0.7 μg/mL)), respectively. In conclusion, these findings are regarded as the first vision of the phytochemical constituents and biological activities of different Hibiscus aqueous extracts. Hibiscus deflersii aqueous extract might be a hopeful origin of functional constituents with anticancer (on A-549 cell line), antidiabetic, and antiobesity activities. It might be a natural alternative remedy and nutritional policy for diabetes and obesity treatment without negative side effects. Isolation of the bioactive phytochemicals from the aqueous extracts of aerial parts of Hibiscus calyphyllus, Hibiscus micranthus, and Hibiscus deflersii and estimation of their biological effects are recommended in further studies.
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Portillo-Torres, Lizbeth Anahí, Aurea Bernardino-Nicanor, Carlos Alberto Gómez-Aldapa, Simplicio González-Montiel, Esmeralda Rangel-Vargas, José Roberto Villagómez-Ibarra, Leopoldo González-Cruz, Humberto Cortés-López, and Javier Castro-Rosas. "Hibiscus Acid and Chromatographic Fractions from Hibiscus Sabdariffa Calyces: Antimicrobial Activity against Multidrug-Resistant Pathogenic Bacteria." Antibiotics 8, no. 4 (November 11, 2019): 218. http://dx.doi.org/10.3390/antibiotics8040218.
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The anti-microbial properties of acetone extracts from Hibiscus sabdariffa calyces, fractions isolated by silica gel chromatography and hibiscus acid purified from some of these fractions and additionally identified by nuclear magnetic resonance spectroscopy, mid-infrared spectroscopy and X-ray diffraction, were studied against both multidrug-resistant Salmonella strains and pathogenic Escherichia coli bacteria. Gel diffusion was used to determine the anti-microbial effects. The mode of action of hibiscus acid was determined by crystal violet assay. Hibiscus acid and 17 of the 25 chromatographic fractions obtained, displayed an anti-microbial effect against all bacterial strains tested. Hibiscus acid showed a greater anti-microbial effect than the acetone extract against most of the bacteria strains, while chromatographic fractions IX–XIV exerted the greatest anti-microbial effect against all bacteria. The minimum inhibitory concentration of the acetone extract was 7 mg/mL, and the minimum bactericidal concentration was 10 mg/mL, while the corresponding values for hibiscus acid were 4–7 and 7 mg/mL, respectively. The results of the crystal violet assay indicate that hibiscus acid alters membrane permeability. Hibiscus acid is a potential alternative to control multidrug-resistant bacteria. Due to its ready availability and easy extraction from H. sabdariffa, hibiscus acid is potentially useful in the food industries.
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Okoduwa, Stanley I. R., Lovina O. Mbora, Matthew E. Adu, and Ameh A. Adeyi. "Comparative Analysis of the Properties of Acid-Base Indicator of Rose (Rosa setigera), Allamanda (Allamanda cathartica), and Hibiscus (Hibiscus rosa-sinensis) Flowers." Biochemistry Research International 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/381721.
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The need to develop effective alternative for synthetic indicators is the demand of present-day chemistry. The acid-base indicator properties of Rose (Rosa setigera), Allamanda (Allamanda cathartica), and Hibiscus (Hibiscus rosa-sinensis) flowers were examined. Colour pigments were extracted from the flowers via cold and solvent extraction using soxhlet extractor. The pH value of the extracts with wavelengths of absorption was determined using ultraviolet spectrophotometer. From the results obtained, all the extracts exhibited sharp contrast between their colours in acid and base. Their pH was found to be 5.5 for cold extract of Rose and 5.6 for solvent extraction, 5.24 for cold extract of a Hibiscus and 6.52 for solvent extraction, 5.35 for cold extract of Allamanda, and 5.45 for solvent extraction. The maximum wavelengths of absorption obtained for all the extract fall within the visible region of electromagnetic spectrum. These values are almost similar to that obtained from synthetic indicators. It is on these bases that we concluded that natural indicators could be an excellent replacement for synthetic indicators since they are cheap, readily available, simple to extract, not toxic, user and environmentally friendly.
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Firmasari, Firmasari, Ni Ketut Sumarni, Ruslan, and Hardi Ys. "PERSEN DEGRADASI EKSTRAK ETANOL KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) SELAMA PENYIMPANAN THE PERCENTAGE OF ETHANOL EXTRACTS OF ROSELLE PETALS (Hibiscus sabdariffa L.) DURING STORAGE." KOVALEN: Jurnal Riset Kimia 5, no. 2 (August 31, 2019): 132–38. http://dx.doi.org/10.22487/kovalen.2019.v5.i2.9970.
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Ethanol extract of roselle petals (Hibiscus sabdariffa L.) is more stable in low pH than in high pH. The research aims to monitor and determine the effect of ratio content in ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce and the pH effect on the percentage of ethanol extract degradation during storage. Two independent variables implemented in this study, are ratio content of ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce and pH. Ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce with ratio content 0,5:1; 1:1; and 1:0 (w/w) and adjusted to pH 2, pH 3, pH 4, pH 5 and pH 6, respectively were investigated. The absorbance measurement of Ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce was depend on each treatment, then analyzed using completely randomized design. The percentage of ethanol extract of roselle petals (Hibiscus sabdariffa L.) degradation in tomato sauce decreased in ratio content 1:0 (w/w) at pH 2 with percentage content of degradation is 30.09%. Furthermore, after 10 days of storage, the highest percentage of ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce degradation is 93.33% with ratio content is 0.5:1 at pH 6 and the lowest is 30.09% with ratio content 1:0 at pH 2.
Keywords: Roselle petals, Ethanol Extract, Tomato Sauce, Percentage of Degradation
Dissertations / Theses on the topic "Hibiscus extract"
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Tallamini, Stéfano Caon. "Avaliação de bactérias psicrotróficas presentes em presunto cozido fatiado e a influência de fatores ambientais na manutenção da qualidade microbiológica e inocuidade do alimento." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/153373.
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Este estudo objetivou analisar o potencial de deterioração da microbiota psicrotrófica presente em presunto cozido fatiado comercializado entre Maio e Junho de 2015 e Fevereiro e Março de 2016 no mercado público da cidade de Porto Alegre/RS e avaliar a influência de fatores ambientais na qualidade microbiológica do mesmo. Os presuntos foram coletados em 4 bancas desse local e foram realizadas contagens de bactérias psicrotróficas de 8 amostras e pesquisa de Listeria monocytogenes. Selecionaram-se 134 colônias de psicrotróficos isolados de presunto fatiado, 71 deles apresentaram atividade proteolítica, 58 atividade lipolítica e 12 apresentaram produção de exopolissacarídeo. Selecionaram-se 2 bactérias com a presença dessas atividades para identificação molecular, as quais foram identificadas como Kluyvera sp. e Carnobacterium sp. Além delas, mais 2 Listeria monocytogenes isoladas nesse trabalho foram submetidas ao teste de produção de biofilme, resultando como fracas formadoras e também ao teste de aderência em aço inoxidável, todas apresentando capacidade de adesão. A pesquisa de Listeria monocytogenes nos presuntos fatiados mostrou 100% de presença, sendo que 50% foram identificadas como L. monocytogenes, as quais pertenceram aos sorotipos 1/2a (1), 1/2b (2), 1/2c (2). Realizou-se análise de presunto cozido inteiro, em sua embalagem original, sendo que não foram encontrados micro-organismos Tratou-se o presunto fatiado com extrato de hibisco a 40% e pediocina a 0,5% e 1,0% e realizou-se contagem de mesófilos, psicrotróficos, Listeria spp., S. aureus e E.coli. O extrato de hibisco reduziu a carga desses micro-organismos. Pediocina 0,5% e 1% apresentaram pouca ação frente ao controle de mesófilos, psicrotróficos e E. coli, mas mantiveram a carga de S. aureus controlada e foram eficazes contra Listeria spp. Foram realizadas também contagens para Listeria monocytogenes, E. coli, S. aureus, mesófilos e psicrotróficos em suabes oriundos de fatiador de alimentos, superfície de contato e utensílio utilizados nas bancas do mercado público. Em conclusão grande parte dos psicrotróficos apresentou atividade proteolítica e lipolítica, as quais alteram organolepticamente o alimento. Alguns apresentaram produção de biofilme e capacidade de aderência, fato indesejado, pois sua remoção é mais difícil no ambiente industrial, com isso nota-se que a legislação brasileira apresenta carência na contagem de psicrotróficos em produtos cárneos.This study aimed to evaluate the microbiota present in sliced cooked ham sold in the public market in Porto Alegre/RS and evaluate the ability of compounds with antimicrobial activity of hibiscus extract and pediocin to control the microbiota found. Ham collected was stored refrigerated until to arrive the laboratory for analysis. Psychrotrophic bacteria counts were performed. Were selected 134 colonies of psychrotrophic microorganisms isolated for sliced ham and 71 of them showed proteolytic activity, 58 lipase activity, 12 showed production of exopolysaccharide. Two of these bacteria were selected for molecular identification which were identified as Kluyvera sp. and Carnobacterium sp. These two bacteria plus two Listeria monocytogenes isolated for sliced ham were subjected to testing of biofilm production (resulting as weak forming of biofilm) and were tested for adhesion in stainless steel and all showed this property. The research of Listeria spp. in sliced cooked ham showed 100% of presence, which 50% were identified as L. monocytogenes to serotypes 1/2a (1) 1/2b (2), 1/2c (2). Analysis was carried out of a whole piece of cooked ham in its original packaging and none microorganisms were found. The sliced ham was treated with hibiscus extract of 40% and pediocin of 0.5% and 1.0% and has been mesophilic, psychrotrophic, Listeria spp., S. aureus and E. coli counts The hibiscus extract reduced the quantity of these microorganisms. Pediocin 0.5% and 1,0% had little action against the control of mesophilic, psychrotrophic and E. coli, but in S. aureus counts were controlled bacteria charge and were effective against Listeria spp.. Also counts of Listeria monocytogenes, E. coli, S. aureus, mesophilic and psychrotrophic bacteria were performed from swabs of slicer food, contact surface and food tool (knife or spatula) used in public market stalls. In conclusion, most of the psychrotrophs presented proteolytic and lipolytic activity, which alter organoleptically the food. Some of them have presented biofilm production and adhesion capacity, undesirable fact because when the biofilm is formed is more difficult to remove it in the industrial environment. With this it is showed that the brazilian legislation presents a lack in the research of psychrotrophs in meat products kept refrigerated.
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Ndiaye, Oumoule. "Impacts of Water, Extraction Procedure and Origin on Anthocyanins and Volatile Compositions of Hibiscus Extracts and Freeze-Dried Hibiscus." Thesis, Virginia Tech, 2016. http://hdl.handle.net/10919/88756.
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There has been a lot of interest in Roselle (Hibiscus sabdariffa L.), called Bissap in Senegal, hibiscus recently because of consumer interest in nutraceutical products. However, beverages made from hibiscus have a short self-life due to anthocyanin and flavor degradation. The purpose of our study was first to assess the impacts of water, extraction procedure and origin on the anthocyanins of hibiscus extracts and secondly, to examine the impacts of freeze-drying on the anthocyanins and the volatiles compositions of hibiscus extracts. For the first experiment, a 2x3 factorial design was used with hibiscus calyces from Senegal and Egypt for the factor origin, distilled water and reformulated Dakar (Senegal) water for the second factor water, and then cold and hot extraction procedures were applied. For the second experiment, Senegalese hibiscus was extracted with hot and cold water and one part of each extract was freeze-dried. For both objectives, a ratio of 1:15 w/v (1 kilogram of calyces for 15 liters of water) were used. The time-temperature was 98°C / 30 min for hot and 22°C / 4 hours for cold extractions. The anthocyanins were determined using high performance liquid chromatography (HPLC). And the volatiles were measured using headspace-solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GCMS). Origin and temperature as well as their interaction had significant effects on the anthocyanin contents, with respective p-values of 0.0036 and 0.0025 and 0.0002. Freeze-drying showed no effect on the anthocyanins in cold extracts. In contrast, a significant difference between the hot extract and its freeze-dried product was observed with a p-value of 0.0013. For the flavor compounds, the aroma profiles were different between cold and hot extracts and their instant powders. Globally the results of this study can help in the optimization when processing hibiscus derivatives.MSLFS
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Maciel, Mônica Jachetti. "Avaliação do extrato alcoólico de hibisco (hibiscus sabdariffa l.) como fator de proteção antibacteriana e antioxidante em alimentos." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2011. http://hdl.handle.net/10183/29544.
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O hibisco (Hibiscus sabdariffa L.) além de possuir propriedades antioxidantes e antimicrobianas, é utilizado como planta medicinal e alimento funcional nos países tropicais. Através de Testes de Diluição em Sistema de Tubos Múltiplos determinou-se a Intensidade de Atividade de Inibição Bacteriana (IINIB/Bacteriostasia) e a Intensidade de Atividade de Inativação Bacteriana (IINAB/Bactericidia) de extrato alcoólico de dois acessos de hibisco, a saber: Palmares do Sul/RS e Porto Alegre/RS sobre inóculos padronizados de Enterococcus faecalis (ATCC 19433), Escherichia coli (ATCC 11229), Salmonella Enteritidis (ATCC 11076) e Staphylococcus aureus (ATCC 25923). Paralelamente, o teor de polifenóis totais e de antocianinas nos cálices e nos frutos com sementes do hibisco foi determinado. A atividade antimicrobiana do extrato alcoólico de cálices, em ambos os acessos, apresentou diferença positiva significativa quando relacionada ao extrato alcoólico dos frutos com sementes. Salmonella Enteritidis foi a bactéria mais sensível ao extrato alcoólico de cálices do hibisco enquanto a mais resistente foi Staphylococcus aureus, independente da variável acesso, considerando somente a estrutura vegetal. Em relação ao extrato alcoólico dos frutos com sementes, Escherichia coli demonstrou a maior sensibilidade e Staphylococcus aureus a maior resistência. Os valores de polifenóis totais e de antocianinas do extrato alcoólico de cálices apresentaram diferença significativa entre si e foram superiores ao extrato alcoólico dos frutos com sementes. Possivelmente existe uma relação direta entre a concentração de antocianina e a atividade antibacteriana em diferentes estruturas vegetais do hibisco.The hibiscus (Hibiscus sabdariffa L.) has antioxidant and antimicrobial properties and it is utilized as functional food and medicinal plant in tropical countries. Through of Dilution Testing in Multiple Tubes System, it were determined the intensity of bacterial inhibition activity (IINIB/ Bacteriostasy) and the intensity of bacterial inactivation activity (IINAB/ Bactericidie) of alcoholic extracts of two accesses of hibiscus, known as: Palmares do Sul/RS and Porto Alegre/RS on standardized inoculum of Enterococcus faecalis (ATCC 19433), Escherichia coli (ATCC 11229), Salmonella Enteritidis (ATCC 11076) and Staphylococcus aureus (ATCC 25923). At the same time, the total content of polyphenols and anthocyanins in the calyxes and fruits with seeds hibiscus was determined. The antimicrobial activity of alcoholic extract of the calyxes in both accesses showed a significant positive difference when related to the alcoholic extract of the fruits with seeds. Salmonella enteritidis was the most sensitive bacteria to the alcoholic extract of calyxes of the hibiscus while the most resistant was Staphylococcus aureus, independent of the variable access, considering only the plant structure. In relation to the alcoholic extract of the fruits with seeds, Escherichia coli showed the highest sensitivity and Staphylococcus aureus the highest resistence. Total polyphenols and anthocyanins of alcoholic extract of calyxes‟s values showed a significant difference and they were superior to alcohol extract of fruits with seeds. Possibly there is a direct relationship between the concentration of anthocyanin and antibacterial activity in different structures of the hibiscus plant.
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Sinela, André Mundombe. "Etude des mécanismes réactionnels et des cinétiques de dégradation des anthocyanes dans un extrait d’Hibiscus sabdariffa L." Thesis, Montpellier, SupAgro, 2016. http://www.theses.fr/2016NSAM0036/document.
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Les calices séchés d’Hibiscus sabdariffa L. sont traditionnellement utilisés en Afrique pour la production de boissons par macération dans l’eau. Cette matière première, riche en anthocyanes, est aussi connue dans l’industrie agro-alimentaire comme source de colorants naturels. Toutefois, la couleur des extraits obtenus évoluent rapidement au cours du temps ce qui nuit au développement de ces produits. Afin de mieux caractériser et de mieux comprendre l’instabilité de la couleur, ce travail propose une étude cinétique et mécanistique de la dégradation des anthocyanes durant la conservation d’un extrait d’H. sabdariffa. La caractérisation biochimique de l’extrait a notamment permis d’identifier les polyphénols majoritaires (delphinidine 3-O-sambubioside Del-3Sb, cyanidine 3-O-sambubioside Cya-3Sb, acides caféoylquiniques) et à mis en évidence la présence de métaux connus comme potentiels catalyseurs d’oxydation (Fe, Cu, Mn). Les cinétiques de dégradation des anthocyanes dans l’extrait ont été suivies par CLHP-DAD entre 4 et 37°C pendant 60 j. Elles peuvent être représentées par un modèle d’ordre 1. La Del-3Sb (Ea= 90 kJ.mol-1) est plus sensible à l’augmentation de la température que la Cya-3Sb (Ea= 80 kJ.mol-1). L’influence de différents facteurs sur la dégradation des anthocyanes a été étudiée dans des solutions modèles simplifiées. La constante de vitesse de dégradation de la Del-3Sb en solution modèle contenant du Fe (III), Cu (II) et Mn (II) à des concentrations similaires à celles de l’extrait, est proche de celle mesurée dans l’extrait. Celle de la Cya-3Sb est 40 % plus faible que dans l’extrait. L’augmentation de la concentration en Fe de 1 à 13 mg.L-1 multiplie environ 3 fois les vitesses de dégradation des anthocyanes. L’ajout d’acide chlorogénique à une solution modèle contenant du Fe augmente la vitesse de dégradation de la Cya-3Sb de 42 %. Un effet inverse est observé dans le cas de Del-3Sb (diminution de 47%). La teneur en oxygène dissous n’a pas d’influence sur les vitesses de dégradation. Deux mécanismes de dégradation des anthocyanes ont été mis en évidence : le mécanisme de scission, représentant une voie minoritaire (10% des anthocyanes dégradés) avec production de phloroglucinaldéhyde, d’acide gallique (issu de Del-3Sb) et protocatéchique (issu de Cya-3Sb) et un mécanisme de condensation avec formation de polymères bruns qui est favorisé par l’augmentation de la concentration en Fe et le mélange Fe, Cu et Mn. Les conclusions de l’ensemble du travail aboutissent à des perspectives sur l’étude de l’impact d’autres composés présents dans l’extrait (sucres, acides aminés) ainsi qui l’identification et la quantification des polymères brunsDried calyx of Hibiscus sabdariffa L. are traditionally used in Africa for beverage production by maceration in water. This raw material, rich in anthocyanins, is also known in the food industry as a source of natural dyes. However, the color of extracts obtained quickly changes over time which affects the development of these products. To better characterize and understand the instability of color, this work provides a mechanistic and kinetic study of the degradation of anthocyanins during the conservation of an extract from H. sabdariffa. Biochemical characterization of the extract allowed to identify predominant polyphenols (delphinidin 3-O-sambubioside Del-3Sb, cyanidin 3-O-sambubioside Cya-3Sb and caffeoylquinic acids) and highlighted the presence of metals known as potential oxidation catalysts (Fe, Cu, Mn). Kinetics of degradation of anthocyanins in the extract were followed by HPLC-DAD between 4 and 37 ° C for 60 days. It can be represented by a first order model. Del-3Sb (Ea = 90 kJ mol-1) is more sensitive to temperature increase than Cya-3Sb (Ea = 80 kJ.mol-1). Influence of different factors on the degradation of anthocyanins was studied in simplified model media. Rate constant of degradation (k) of Del-3Sb on model media containing Fe (III), Cu (II) and Mn (II) at similar concentrations to those of the extract was similar to that measured in the extract. For Cya-3Sb it was 40% lower than in the extract. Increasing Fe concentration of 1 to 13 mg.L 1 increased by about 3-fold the k of anthocyanins. Adding chlorogenic acid in a model media containing Fe increased k of Cya-3Sb 42%. Opposite effect was observed for Del-3Sb (47% decrease). Dissolved oxygen content has no influence on k of anthocyanins. Two mechanisms of degradation of anthocyanins have been identified: the scission mechanism, representing a minority pathway (10% of degraded anthocyanins) with production of phloroglucinaldehyde, gallic acid (from Del-3Sb) and protocatechuic (from Cya -3Sb). The other mechanism is condensation with formation of brown polymers, it is promoted by increasing of concentration of Fe was and mixture of Fe, Cu and Mn. Conclusions led to perspectives on study of impact of other compound in the extract (sugar, amino-acids) as well as identification and quantification of brown polymers
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Melecchi, Maria Ines Soares. "Caracterização química de extratos de Hibiscus tiliaceus : estudo comparativo de métodos de extração." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2005. http://hdl.handle.net/10183/6036.
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O presente trabalho teve como objetivo estudar a composição química das flores de Hibiscus tiliaceus L usando diferentes técnicas de extração. Os métodos de extração usados foram maceração, Soxhlet, ultra-som, extração com fluido supercrítico e extração com líquido pressurizado. Foram investigadas as variáveis que podem influenciar no processo de extração tais como temperatura, pressão, polaridade do solvente, tempo de extração e massa de amostra, entre outras. A identificação dos compostos foi realizada por cromatografia gasosa acoplada com detector de espectrometria de massas. Os métodos de extração utilizados mostraram-se eficientes para a obtenção dos extratos, com algumas diferenças qualitativas. Quantitativamente, os compostos majoritários, em todas as técnicas de extração usadas, foram ácidos carboxílicos (C16 e C18), hidrocarbonetos com número ímpar de carbonos (C27, C29 e C31) e fitosteróis. Entre os métodos de extração que utilizam solventes orgânicos, a extração com líquido pressurizado apresentou maior rendimento em massa de extrato e maior concentração de alguns dos compostos presentes nas flores de Hibiscus tiliaceus L., com as vantagens de redução de solventes e do tempo de extração. A variável que se mostrou mais importante nos procedimentos de extração foi a polaridade do solvente. A composição química das flores Hibiscus tiliaceus L apresentou além dos compostos citados, álcoois, aldeídos e vitamina E. A determinação do potencial antioxidante da planta, utilizando o método por quimiluminescência, permitiu encontrar valores para TAR e TRAP superiores ao trolox, utilizado como padrão, indicando a presença de antioxidantes nos extratos.
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Elashi, Balsam. "The antimicrobial and antifungal efficacy of indigenous plant extracts against streptococcus mutans, Escherichia colia and Candida albicans." University of the Western Cape, 2015. http://hdl.handle.net/11394/4246.
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Magister Scientiae Dentium - MSc(Dent)The antimicrobial and antifungal efficacy of indigenous plant extracts against Streptococcus mutans , Escherichia coli and Candida albicans .Aim: To determine the antimicrobial and antifungal efficacy of indigenous plant extracts, Tamarindus Indica (T. ndica), Hibiscus sabdariffa (H. sabdaraffi), Adansonia digitata (A.digitata) and Moringa oleifera (M. oleifera) against Streptococcus mutans ( S. mutans ), Escherichia coli ( E. coli ) and Candida albicans (C. albicans) objectives:The objectives of this study were to: 1.Measure the zones of growth inhibition by T. indicia , A. digitata , M.oleifera and H. sabdariffi extracts against, S.mutans , E.coli and C.albicans . 2.Compare the size of inhibition zones of different bacteria or fungus, S. mutans ,E.coli and C. albicans, around the same plant extract.3.Compare the size of inhibition zones for the same bacteria in different plant extracts T.indicia ,A.digitata H.sabdariffi and M.oleifera Methodology .The antimicrobial and antifungal effect of the ethanolic extracts of T. indica , H.sabdariffa, A.digitata and M.oleifera was performed using the disc diffusion method against S. mutans ,E.coli and C.albicans. The antibacterial and antifungal activity of the plants was determined by measuring the diameter of the inhibition zones. esults and conclusion: The results showed that.T.indica and H.sabdariffa ethanolic extracts have an antibacterial effect against S.mutans and E.coli.However, H.sabdariffi showed a significantly higher antibacterial effect against E.coli and S.mutans, with a range of 14.50mm to 12.01mm and 16.41 mm to 14.39 mm compared to T.indica ,with a range of 11.41 mm to 7.04mm and 6.88mm to 10.40mm, respectively.Furthermore, the statistical multiple pairwise test (Conover Iman procedure/Two -tailed test) omputed that the effect of H.sabdariffi is significantly (critical value >7.229) greater for the G ram positive S.mutans than the G ram negative E.coli.On the other hand,T.indica showed a similar antibacterial effect against S.mutans and E.coli,respectively.In contrast, M. oleifera and A.digitata ethanolic plant extracts showed no antibacterial effect against E.coli and S.mutans. All the indigenous plants tested, T.indica ,H.sabdariffa, M. oleifera and A.digitata had no antifungal activity on C.albicans
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Almeida, Eduardo Farina de. "Efeito antidepressivo e ansiolítico do extrato metanólico de Hibiscus tiliaceus em modelo animal de depressão pós-parto." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/67656.
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Hibiscus tiliaceus L. (Malvaceae) é usado popularmente em desordens do pós-parto. O extrato metanólico de flores de H. tiliaceus apresentou atividade antidepressiva-like nos testes de nado forçado e de suspensão da cauda, ambos são amplamente usados como modelos animais preditivos da atividade antidepressiva. Além disso, o extrato demonstrou uma tendência de aumento do tempo gasto nos braços abertos no labirinto em cruz elevado. Considerando que o extrato de H. tiliaceus, que contém fitoesteróis como o estigmasterol, o stigmastadienol e o stigmastadienone, pode ser útil no tratamento ou prevenção da depressão pós-parto relacionadas à retirada crônica de altos níveis de hormônios associados à gestação. Nosso objetivo foi avaliar o efeito de extratos de H. tiliaceus em modelo animal de depressão pós-parto e ansiedade. Utilizamos ratas Wistar fêmeas submetidas ao modelo de depressão pós-parto induzido por hormônios (estradiol e progesterona), administrados por via subcutânea. Após período gestacional induzido, os animais receberam água, veiculo ou extrato de H. tiliaceus nas doses de 100 e 400mg/kg, via oral (gavagem). No período pós-parto, as ratas foram submetidas a testes comportamentais de nado forçado, o labirinto em cruz elevado e de preferência claro-escuro como modelos preditivo de depressão e ansiedade. Foi obsevado um aumento no tempo de imobilidade associado a uma diminuição na tentativa de fuga, no teste do nado forçado, em animais submetidos ao modelo de depressão pós-parto comparado ao grupo controle, sugerindo que este modelo é capaz reproduzir sintomas de depressão pós-parto. Não houve diferença significativa nos testes de ansiedade entre os animais submetidos ao modelo de depressão pós-parto comparado aos controles. A administração de extrato metanólico de H. tiliaceus não foi capaz de alterar o comportamento relacionado à depressão e ansiedade em ratas Wistar.Postpartum affective disorders are rarely modeled. The depressive-like behavior of hormone withdrawal following hormone-simulated "pregnancy" was described in Long-Evans and Sprague Dawley rats. Our aim was to evaluate the validity of hormone withdrawal following hormonesimulated "pregnancy" method in Wistar rats as a model of depression and/or anxiety. Recently, it was demonstrated an antidepressant-like profile of methanol extract of Hibiscus tiliaceus L., a plant used in postpartum disorders, in adult male Swiss albino mice, then, we also investigated the antidepressant and anxiolytic-like activities of the methanol extract of H. tiliaceus flowers using this animal model of postpartum disorder. Ovariectomized rats received daily injections of the vehicle or hormones (estradiol and progesterone) to simulate the 23-day gestational period in the rat. Days 24-27 were considered the ''post-partum'' period, where the methanolic extract of H. tiliaceus or vehicle were administered by gavage. Rats were submitted to forced swimming, elevated plus-maze test and lightdark box tests. Rats submitted to ''post-partum depression model'' increased the immobility time in forced swimming. The methanolic extract of H. tiliaceus administration did not alter the immobility time in the forced swim test. In the light-dark box test, rats submitted to post-partum depression model showed decreases in number of rearing in dark compartment. In conclusion, our data indicate that Wistar rats may be an adequate model postpartum affective disorders, showing ''depressive-like'' symptoms in the forced swim test without any anxiogenic effect. Besides, we could suggest that decrease on number of rearing in the dark compartment may indicate the motivational state.
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SILVA, Kaliny Veiga Pessoa da. "Prospecção de Geraniol synthase (GES) em espécies aromáticas e uso do óleo de kenaf para controle da ramulose no algodoeiro." Universidade Federal Rural de Pernambuco, 2016. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7179.
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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
Cotton stands out as a high value textile plant in the world market due to the quality of its fibers and the commercial derivatives that the plant produces. Ramulose is a disease that causes great damage to this crop, caused by the fungus Colletotrichum gossypii South var. cephalosporioides Costa, mainly affects young leaves, the most common symptoms being the darkening and necrosis of leaves and branches, which may compromise the whole development of the plant. Extracts and vegetable oils have been studied as an alternative to control pests and diseases such as ramulose in crops. These have as main constituents the secondary compounds, chemicals known as phytochemicals, terpenoids, alkaloids and glycosides. Among these the geraniol, monoterpene that stands out for its fungicidal property. Kenaf (Hibiscus cannabinus) belongs to the family Malvaceae and is rich in compounds known as polyphenols, alkaloids, tannins and essential oils, also being a promising source of fungicidal oil. This work aimed to analyze the effect of oils and extracts on the development of the fungus C. gossypii, which causes ramulose, by means of two researches: In the first one, we sought to prospect Geraniol synthase, the precursor enzyme of geraniol, in aromatic species, for means of molecular analyzes via PCR and qPCR, besides phytopathological tests, in order to evaluate the geraniol expression and the effectiveness of its extracts and vegetable oils in the prevention and control of ramulose; The second study evaluated the effect of Kenaf oil on the development of fungus C. gossypii under laboratory conditions. In order to carry out the first research eleven aromatic species were evaluated, among them M. pulegium, O. basilicum, O. majorana and R. officinalise, which presented satisfactory levels of expression of this enzyme in the molecular assays, being selected for bioassays in vivo against C. gossypii. O. basilicum, M. pulegium and O. majorana oils, at 2000 ppm concentration, were tested in vivo and their preventive and curative actions compared to commercial fungicide. There was effective control of the oils O. basilicum and M. pulegium under ambient conditions, and M. Polegium was distinguished with D.S.I. around 30%, lower value than that presented by the commercial fungicide (40%). These results indicate that the use of aromatic species can be an effective alternative for the treatment of ramulose, with the advantages of being a low cost method and not presenting risks of environmental contamination. In addition, the genetic characterization of aromatic species is an important source of information to expand the use of natural resources, allowing new materials that hold genes of interest to be found and serve as environmental conservation strategies. In the second study, phytopathological tests were performed on isolate 287 of C. gossypii on the effect of Kenaf oil at concentrations of 25, 50 and 75 μl / 1 ml. It was verified by regression analysis that there was no reduction in the mycelial growth of C. gossypii in the oil concentrations adopted in this work. The data presented by fungus growth, fungistatic effect and spore production did not reveal a statistical difference between the treatment with oil and control. Based on the composition of kenaf oil, rich in fungicidal ingredients, even this oil lacking control over C. gossypii in the present study, it is believed that in higher concentrations this oil may exert an effect on this pathogen.
O algodoeiro destaca-se como uma planta têxtil de alto valor no mercado mundial devido à qualidade de suas fibras e aos derivados comerciais que a planta produz. A ramulose é uma doença que causa grandes danos a esta cultura, causada pelo fungo Colletotrichum gossypii South var. cephalosporioides Costa, afeta principalmente folhas jovens, sendo os sintomas mais comuns o escurecimento e necrose das folhas e ramos, podendo comprometer todo o desenvolvimento da planta. Extratos e óleos vegetais vêm sendo estudados como alternativa para controle de pragas e doenças como a ramulose em lavouras. Estes têm como principais constituintes os compostos secundários, substâncias químicas conhecidas como fitoquímicos, terpenóides, alcalóides e glicosídeos. Dentre estes o geraniol, monoterpeno que destaca-se por sua propriedade fungicida. Já o Kenaf (Hibiscus cannabinus) pertence à família Malvaceae e é rica em compostos conhecidos como polifenóis, alcalóides, taninos e óleos essenciais, também sendo uma promissora fonte de óleo fungicida. Este trabalho buscou analisar o efeito de óleos e extratos sobre o desenvolvimento do fungo C. gossypii, causador da ramulose, por meio de duas pesquisas: Na primeira, buscou-se prospectar a Geraniol synthase, enzima precursora do geraniol, em espécies aromáticas, por meio de análises moleculares via PCR e qPCR, além de testes fitopatológicos, a fim de avaliar a expressão do geraniol e a eficácia de seus extratos e óleos vegetais na prevenção e no controle da ramulose; Na segunda pesquisa avaliou-se o efeito do óleo de Kenaf sobre o desenvolvimento do fungo C. gossypii em condições de laboratório. Para realização da primeira pesquisa onze espécies aromáticas foram avaliadas, entre elas M. pulegium, O. basilicum, O. majorana e R. officinalise, as quais apresentaram níveis satisfatório de expressão desta enzima nos ensaios moleculares, sendo selecionadas para bioensaios em in vivo contra C. gossypii. Os óleos de O. basilicum, M. pulegium e O. majorana, na concentração de 2000 ppm, foram testados in vivo e suas ações preventivas e curativas comparadas com fungicida comercial. Houve controle efetivo dos óleos O. basilicum e M. pulegium em condições ambiente, sendo que M. Polegium destacou-se com D.S.I. em torno de 30%, valor menor do que o apresentado pelo fungicida comercial (40%). Esses resultados denotam que o uso de espécies aromáticas pode se constituir numa alternativa eficaz para o tratamento da ramulose, com as vantagens de ser um método de baixo custo e de não fornecer riscos de contaminação ambiental. Além disso, a caracterização genética de espécies aromáticas é uma importante fonte de informações para ampliar a utilização dos recursos naturais possibilitando que novos materiais detentores de genes de interesse sejam encontrados e sirvam como estratégias de conservação ambiental. Na segunda pesquisa, testes fitopatológicos foram realizados no isolado 287, de C. gossypii, sobre o efeito do óleo de Kenaf em concentrações de 25, 50 e 75 μl/1ml. Verificou-se por análise de regressão que não houve redução no crescimento micelial de C. gossypii nas concentrações de óleo adotadas neste trabalho. Os dados apresentados pelo crescimento de fungos, efeito fungistático e produção de esporos não revelaram diferença estatística entre o tratamento com óleo e controle. Com base na composição do óleo de kenaf, rica em ingredientes fungicida, mesmo este óleo não apresentando controle sobre C. gossypii no presente estudo, acredita-se que em concentrações mais elevadas este óleo possa exercer efeito sobre este patógeno.
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SANTANA, LEGORRETA SERGIO 443157, and LEGORRETA SERGIO SANTANA. "Diseño de partículas a base de inulina de Dalia (Dahlia variabilis Cav.) para la liberación controlada de un extracto de Jamaica (Hibiscus sabdariffa L.)." Tesis de maestría, Universidad Autónoma del Estado de México, 2017. http://hdl.handle.net/20.500.11799/67793.
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0La dalia (Dahlia coccinea Cav.) es una planta que ha sido cultivada y mejorada genéticamente con fines ornamentales; no obstante, su sistema radicular almacena carbohidratos de reserva bajo la forma de inulina y otros fructanos. La inulina forma parte de la fibra dietética de diversos vegetales y es considerada como un compuesto prebiótico. Este polisacárido se extrae principalmente de la achicoria y la alcachofa mediante métodos de separación muy variados, siendo la raíz de la dalia una opción interesante de explotación industrial. El propósito de este estudio fue proponer un método simple de extracción de inulina de la raíz de dalia y evaluar el efecto del cultivo de dalias silvestres sobre su contenido de inulina, fructanos, fructosa y glucosa. Se realizaron colectas de dalia silvestre y se propuso un método de extracción de inulina. Las semillas de los ejemplares recolectados fueron cultivadas y a las raíces obtenidas se les determinó la concentración de fructanos, inulina, fructosa y glucosa y se estimó el grado de polimerización. La relación tiempo-temperatura afecta significativamente la concentración de inulina y fructanos extraídos, siendo la combinación óptima 80° C durante 60 minutos. La influencia del pH resultó no significativa para este mismo propósito. Por otro lado, el cultivo de las dalias silvestres aumenta significativamente el contenido de inulina y fructanos de sus raíces, aunque el grado de polimerización, estimado a partir de la relación fructosa/glucosa, disminuye. La liberación de polifenoles encapsulados en inulina en una solución isotónica sigue una cinética de primer orden con una constante de velocidad (basada en la absorbancia del medio isotónico) de 0.0148 unidades por minuto y una liberación máxima de 0.4166 unidades de absorbancia que se observaron a las 8 horas de permanencia de las partículas en la solución isotónica.
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Soares, Gustavo Forlani. "Atividade da Paraoxanase 1 em pacientes oncológicos caninos submetidos a terapia com agentes antineoplásicos e avaliação do balanço oxidativo e parâmetros hematopoiéticos de ratas submetidas a Carboplatina e tratadas com extrato aquoso de Triticum aestivum e Hibiscus rosa-sinensis." Universidade Federal de Pelotas, 2016. http://repositorio.ufpel.edu.br:8080/handle/prefix/3602.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
Os neoplasmas estão entre as afecções de maior importância na medicina humana e veterinária. Entre as diretrizes terapêuticas mais utilizadas está a administração de agentes antineoplásicos como a carboplatina, a qual é eficaz no tratamento de carcinomas, entretanto possui efeitos colaterais, como a mielossupressão e a liberação excessiva de radicais livres. Nesse contexto, objetivou-se avaliar o balanço oxidativo de cães submetidos a quimioterapia, assim como a utilização dos extratos aquosos de Triticum aestivum (trigo) e de Hibiscus rosa-sinensis (HRS) como adjuvante na terapia antineoplásica com carboplatina em modelo murino. Para avaliação do balanço oxidativo em caninos foram selecionados oito cães, quatro com câncer e quatro hígidos, sendo mensurada a atividade da Paraoxanase 1 (PON1) no momento que antecedeu a aplicação do agente antineoplásico (dia 0) e sete dias após a aplicação (dia 7). Foi constatado que no dia zero os pacientes oncológicos caninos (G1) apresentaram menor atividade da PON1 do que os cães do (G2) (p<0,05), além disso, a atividade dessa enzima foi ainda mais comprometida após a administração de fármacos antineoplásicos (p<0,05), sugerindo a necessidade de administração de compostos antioxidantes para esses pacientes. Para avaliar o potencial dos extratos aquosos de trigo e HRS como adjuvantes na terapia antineoplásica, foram utilizadas 48 ratas, aleatoriamente distribuídas em seis grupos: G1 - testemunha tratado com solução fisiológica; G2 - controle tratado com solução fisiológica; G3 - tratado com 100 mg/kg de extrato aquoso de trigo; G4 - tratado com 400 mg/kg de extrato aquoso de trigo; G5 - tratado com 125mg/kg de extrato aquoso de HRS; e G6 - tratado com 250mg/kg de extrato aquoso de HRS. Administrou-se 300 mg/m² de carboplatina por via intraperitoneal no dia 0 nos grupos G2, G3, G4, G5 e G6, enquanto o G1 recebeu 1mL de solução fisiológica pela mesma via. Os animais foram tratados diariamente durante 21 dias por gavagem orogástrica de acordo com seu grupo experimental. Foi coletado sangue dos animais para realização de hemograma e avaliação dos parâmetros bioquímicos alanina aminotransferase, fosfatase alcalina, ureia e creatinina nos dias três, sete e 21, com a avaliação da atividade da Paroxanase 1 no dia três. No dia 21 foi realizada a eutanásia das ratas e coletado aspirados da medula óssea para realização do mielograma e fragmentos do rim e do fígado para análise histológica. Os efeitos adversos da carboplatina foram confirmados pelas alterações observadas no G2 quando comparado ao G1. Foram observados resultados promissores com relação ao balanço oxidativo no G3, G4, G5 e G6, devido aos animais desse grupo apresentarem atividade da PON1 superior ao G2 (p<0,05). Os mesmos grupos apresentaram contagem de leucócitos totais superiores ao G2, sendo significativamente superior no G4, G5 e G6 no dia 21 (p<0,05). Os mielogramas dos animais tratados com extratos vegetais não apresentaram as evidentes alterações de parâmetros que foram observadas no G2 (queda de células eritróides e aumento de neutrófilos segmentados e promielócitos) (p<0,05), indicando uma compensação do efeito da carboplatina. Os parâmetros bioquímicos e histopatológicos avaliados encontravam-se dentro do esperado para espécie em todos os animais. Nas condições deste estudo, os extratos aquosos de trigo e Hibiscus rosa-sinensis mostraram-se seguros nas doses utilizadas e uteis no tratamento da mielossupressão e liberação excessiva de radicais livres induzidas pelo fármaco carboplatina.
Cancer is one of the most important diseases in human and veterinary medicine. Among the most commonly used therapeutic guidelines is the administration of anticancer agents such as carboplatin, which is effective in treating carcinomas, but has side effects such as myelosuppression and excessive release of free radicals. This study proposed to assess the oxidative balance of dogs undergoing chemotherapy, as well as the use of aqueous extracts of wheat and Hibiscus rosa-sinensis (HRS) as adjuvants to antineoplastic therapy with carboplatin in rats. To assess the oxidative balance of dogs, eight animals were selected, four cancer patients and four healthy witnesses, and the seric Paraoxanase 1 (PON1) activity was measured before and after the application of antineoplastic agents. Cancer patients were found to exhibit lower activity of PON1 (p<0.05), furthermore, the activity of this enzyme was further compromised after the administration of anticancer drugs (p<0.05). To assess the potential of aqueous extracts of wheat and HRS as adjuvants in antineoplastic therapy, 48 rats were randomly divided into six groups: G1 - witness treated with saline solution; G2 - control treated with saline solution; G3 - treated with 100 mg/kg of wheat aqueous extract; G4 - treated with 400 mg/kg of wheat aqueous extract; G5 - treated with 125mg/kg of HRS aqueous extract; and G6 - treated with 250 mg/kg of HRS aqueous extract. Three hundred mg/m² of carboplatin was administered intraperitoneally on day zero in groups G2, G3, G4, G5 and G6, while the G1 received 1mL of saline. The animals were treated daily for 21 days by orogastric gavage according to their experimental group. Blood samples were collected from animals for CBC, and blood biochemistry on days three, seven, and 21, Paroxanase1 was assessed on day three. On day 21 the animals were euthanaized and bone marrow, kidney, liver tissues were collected. The adverse effects of carboplatin were confirmed by the changes observed in G2 when compared to G1. Promising results were observed with respect to the oxidative balance in G3, G4, G5 and G6. Animals in these groups present activity of the PON 1 higher than the G2 (p <0.05). The same groups had higher counts of total leukocytes than G2, being significantly higher in G4, G5, and G6 on day 21 (p <0.05). The myelograms of animals treated with plant extracts did not show the overt parameter changes that were observed in G2 (reduction of erythroid cells and increased segmented neutrophils and promyelocytes) (p<0.05), indicating a compensation of the effect of carboplatin . Biochemical and histopathological parameters were within the expected range for the species in all animals. In our study, the aqueous extracts of wheat and HRS proved to be safe at the doses used and useful in the treatment of myelosuppression and excessive release of free radicals induced by the drug carboplatin.
Book chapters on the topic "Hibiscus extract"
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Bährle-Rapp, Marina. "Hibiscus Militaris Extract." In Springer Lexikon Kosmetik und Körperpflege, 257. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_4744.
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Bährle-Rapp, Marina. "Hibiscus Syriacus Extract." In Springer Lexikon Kosmetik und Körperpflege, 257. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_4747.
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Bährle-Rapp, Marina. "Hibiscus Rosa-Sinensis Extract." In Springer Lexikon Kosmetik und Körperpflege, 257. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_4745.
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Idham, Zuhaili, Nicky Rahmana Putra, Nor Faadila Mohd Idrus, Norlisa Mili, Muhammad Syafiq Hazwan Ruslan, Noor Azwani Mohd Rasidek, and Mohd Azizi Che Yunus. "Characteristics and Empirical Modelling of Extract from Hibiscus sabdariffa Using Supercritical CO2 Extraction with Ethanol-Water as Modifier." In Lecture Notes in Mechanical Engineering, 375–88. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-0742-4_26.
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Anand, Akancha, and Biswatrish Sarkar. "Phytochemical Screening and Antioxidant Property of Anthocyanins Extracts from Hibiscus rosa-sinensis." In Applications of Biotechnology for Sustainable Development, 139–47. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-5538-6_17.
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Redzuan, Syafiqah, Chai Yee Ho, Zuhaili Idham, Suzana Yusuf, Nicky Rahman Putra, Mohd Azizi Che Yunus, Syamila Mansor, and Muhammad Syafiq Hazwan Ruslan. "Optimization of Anthocyanins Extracts from Roselle (Hibiscus sabdarifa) Petals Using Ultrasonic-Assisted Extraction Method." In Lecture Notes in Mechanical Engineering, 295–309. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-0742-4_21.
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Quansah, Lydia, Gustav Komla Mahunu, Haroon Elrasheid Tahir, Maurice Tibiru Apaliya, Mildred Osei-Kwarteng, and Abdalbasit Adam Mariod. "Hibiscus sabdariffa extract: antimicrobial prospects in food pathogens and mycotoxins management." In Roselle (Hibiscus sabdariffa), 215–30. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-822100-6.00003-3.
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Karim, Roselina, Nor Aini Mat Noh, Shafa’atu Giwa Ibrahim, Wan Zunairah Wan Ibadullah, Norhasnida Zawawi, and Nazamid Saari. "Kenaf (Hibiscus cannabinus L.) Seed Extract as a New Plant-Based Milk Alternative and Its Potential Food Uses." In Milk Substitutes [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.94067.
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Kenaf (Hibiscus cannabinus L.) seed is rich in protein, fat, fiber, and other essential nutrients. Kenaf seed comprises of high protein (22–31%) and oil (22–25%) contents which suggested its high potential food application. This chapter discusses the potential and early development of kenaf-based plant-milk and tofu. The step-by-step processes involved in preparation of kenaf-based milk and kenaf-based tofu at laboratory-scale are illustrated. Soaking conditions (temperature and time) of kenaf seed as pretreatment in preparation of kenaf seed milk were highlighted. Hydration of kenaf seed were found to be faster at elevated temperature, however higher soaking temperature and prolonged soaking time causes some losses of protein (%) and solid content (%) which are unfavorable for production of highly nutritious plant-based milk. Furthermore, in preparation of kenaf-based tofu, soaking temperature of seed also affected the properties of the tofu. As the soaking temperature was increased from 25–65°C, the yield, hardness, and chewiness of kenaf tofu decreased. It was recommended that soaking of kenaf seed at 25°C and the use of aluminum potassium salt at 1.00 g% as coagulant produces kenaf-based tofu with optimum quality.
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Mahunu, Gustav Komla, Haroon Elrasheid Tahir, Mildred Osei-Kwarteng, Abdalbasit Adam Mariod, and Joseph Patrick Gweyi-Onyango. "Food use of whole and extracts of Hibiscus sabdariffa." In Roselle (Hibiscus sabdariffa), 123–36. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-822100-6.00009-4.
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Santos, Davi Vicente dos, Marcia Maria Dourado Maranhão, Naomi Kato Simas, Taiane Borges Machado Silva, Gláucio Diré Feliciano, and Alaíde de Sá Barreto. "ESTUDO QUÍMICO E BIOLÓGICO DO EXTRATO AQUOSO HIBISCUS SABDARIFFA (MALVACEAE)." In Produção e Controle de Produtos Naturais 2, 72–83. Atena Editora, 2020. http://dx.doi.org/10.22533/at.ed.0012009047.
Full textConference papers on the topic "Hibiscus extract"
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Güler, Nilgün. "Staining effect of Hibiscus sabdariffa extract on Human Blood Cells." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-71.
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Rao, B. Lakshmeesha, G. Parameshwara Gouda, and C. S. Shivananda. "Green synthesis of silver nanoparticles using Hibiscus Rosa Sinensis flower extract." In 3RD INTERNATIONAL CONFERENCE ON CONDENSED MATTER AND APPLIED PHYSICS (ICC-2019). AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0002996.
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Nuri, Md Touhidul Islam, and A. K. M. Bazlur Rashid. "Green synthesis of silver nanoparticles using hibiscus leaf extract and its characterization." In 2015 International Conference on Electrical Engineering and Information Communication Technology (ICEEICT). IEEE, 2015. http://dx.doi.org/10.1109/iceeict.2015.7307361.
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"Antioxidant, Antiglycation, and Antiaging Activity of Roselle Petals Extract (Hibiscus sabdariffa L.)." In August 8-10, 2018 Pnom Penh (Cambodia). Dignified Researchers Publication, 2018. http://dx.doi.org/10.17758/dirpub4.dir0818207.
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Othman, Maizatulnisa, Afiqah Athirah Yusup, Nazarudin Zakaria, and Khalisanni Khalid. "Bio-polymer chitosan and corn starch with extract of hibiscus rosa-sinensis (hibiscus) as PH indicator for visually-smart food packaging." In DISRUPTIVE INNOVATION IN MECHANICAL ENGINEERING FOR INDUSTRY COMPETITIVENESS: Proceedings of the 3rd International Conference on Mechanical Engineering (ICOME 2017). Author(s), 2018. http://dx.doi.org/10.1063/1.5047198.
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Arias, Ruth, Jairo Silva-Chango, Willan Caicedo, Luis Bravo-Sánchez, and Ruth Arias-Gutiérrez. "Use of Hibiscus sabdariffa extract as a natural antioxidant in sausages." In MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition. Basel, Switzerland: MDPI, 2019. http://dx.doi.org/10.3390/mol2net-05-06400.
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Djaeni, M., F. D. Utari, and A. C. Kumoro. "Hibiscus sabdariffa L extract drying with different carrier agent: Drying rate evaluation and color analysis." In PROCEEDINGS FROM THE 14TH INTERNATIONAL SYMPOSIUM ON THERAPEUTIC ULTRASOUND. Author(s), 2017. http://dx.doi.org/10.1063/1.4978118.
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Gomesh, N., R. Syafinar, S. Dayang, C. T. Loh, G. H. Yeap, and B. P. Chang. "Optical comparison of dye-sensitized solar cell using nature based dye extract from hibiscus and bougainvillea." In 2016 IEEE International Conference on Power and Energy (PECon). IEEE, 2016. http://dx.doi.org/10.1109/pecon.2016.7951639.
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Ukkund, Shareefraza J., Darshanram, Zubear Khan, Sanjay, Ravika Patil, Apoorva Udupa, Abhinaya Nellerichale, and Prasad Puthiyillam. "Microwave assisted green synthesis and characterization of silver nanoparticles from Hibiscus leaf extract and investigation of their antimicrobial activities." In EMERGING TRENDS IN MECHANICAL ENGINEERING 2018. Author(s), 2019. http://dx.doi.org/10.1063/1.5092885.
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Fairuzi, Afiza Ahmad, Noor Najmi Bonnia, Rabiatuladawiyah Md Akhir, Hazizan Md Akil, Sabrina M. Yahya, and Norafifah A. Rahman. "Comparison studies on catalytic properties of silver nanoparticles biosynthesized via aqueous leaves extract of Hibiscus rosa sinensis and Imperata cylindrica." In 8TH INTERNATIONAL CONFERENCE ON NANOSCIENCE AND NANOTECHNOLOGY 2017 (NANO-SciTech 2017). Author(s), 2018. http://dx.doi.org/10.1063/1.5036858.
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