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Putra, Imam Budi, Nelva K. Jusuf, and Imam Bagus Sumantri. "The Potency of Hibiscus rosa-sinensis Linn. Leaves Ethanol Extract as Hair Growth." Open Access Macedonian Journal of Medical Sciences 8, A (February 15, 2020): 89–92. http://dx.doi.org/10.3889/oamjms.2020.4211.
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INTRODUCTION: Hair is one of the organs and crowns for all people. Hair loss will really bother for most people. One of the plants that can be used as hair growth is Hibiscus rosa-sinensis Linn.
OBJECTIVE: The objective of the study was to find the potency of H. rosa-sinensis leaves ethanol extracts as hair growth.
METHODS: In this research, simplicia and extracts were made from hibiscus leaves using ethanol as a solvent. Simplicia and extracts were tested for phytochemical screening and characterization of each. The extract obtained was tested in vivo with animals test by observing mouse’s hair growth for 25 days of observation.
RESULTS: Simplicia and ethanol extract of Hibiscus leaves contain flavonoids, alkaloids, glycosides, tannins, and steroids/terpenoids. The characteristics of Hibiscus leaves simplicia and extract had water content (9.71% vs. 10.65%), water-soluble extract content (19.91% vs. 27.58%), ethanol-soluble extract content (37.86% vs. 49.51%), total ash content (7.96% vs. 9.46%), and acid-insoluble ash content (0.78% vs. 1.28%). In vivo testing with animals, ethanol extract of Hibiscus leaves had an effect as a hair growth until the 25th day with an average concentration of 2.5% (14 mm), 5% (16 mm), and 10% (19 mm).
DISCUSSION: The extract of Hibiscus leaves that showed the presence of carbohydrate, alkaloid, flavonoid, steroids, protein, tannin, and amino acids. The presence of active constituents such as flavonoids and tannins may be responsible for hair growth activity.
CONCLUSION: The ethanol extracts of H. rosa-sinensis leaves can increase hair growth, at optimum concentrations of 10% extract.
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Márquez-Rodríguez, Ana Selene, Susana Nevárez-Baca, Julio César Lerma-Hernández, León Raul Hernández-Ochoa, Guadalupe Virginia Nevárez-Moorillon, Néstor Gutiérrez-Méndez, Laila Nayzzel Muñoz-Castellanos, and Erika Salas. "In Vitro Antibacterial Activity of Hibiscus sabdariffa L. Phenolic Extract and Its In Situ Application on Shelf-Life of Beef Meat." Foods 9, no. 8 (August 8, 2020): 1080. http://dx.doi.org/10.3390/foods9081080.
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Compounds from spices and herbs extracts are being explored as natural antibacterial additives. A plant extract used in traditional folk medicine is Hibiscus sabdariffa L., also known as Roselle. Therefore, the potential use of a phenolic hibiscus extract as antibacterial or natural food preservative was analyzed in vitro and in situ. A phenolic extract was obtained from hibiscus calyces and fractionated, and then the fractions were tested against foodborne pathogen bacteria. Liquid–liquid extraction and solid-phase extraction were used to fractionate the hibiscus extract, and HPLC was employed to analyze the fractions’ phenolic composition. Minimum bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) were calculated for brute hibiscus phenolic extract, each of the fractions and pure commercial phenolic compounds. Bacteria tested were Escherichia coli, Salmonella enterica serovar Typhimurium, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. The fraction obtained after liquid–liquid extraction presented the best performance of MBC and MIC against the bacteria tested. Furthermore, a hibiscus ethanolic extract was employed as a natural preservative to extend the shelf-life of beef. Microbiological, color and sensory analyses were performed to the meat during the shelf-life test. The application of the phenolic hibiscus extract also showed an increase of the duration of the meat`s shelf life.
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C, Nwokonkwo Dorothy. "UTILIZATION OF AQUEOUS, METHANOL AND CHLOROFORM EXTRACTS OF LOCAL PLANTS- IXORA COCCINEA AND HIBISCUS SABDARRIFFA (ZOBO) FROM ABAKALIKI AS FABRIC DYES." JOURNAL OF ADVANCES IN CHEMISTRY 11, no. 5 (April 18, 2015): 3576–82. http://dx.doi.org/10.24297/jac.v11i5.4476.
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This investigation dealt with the dyeing and fastness properties of crude constituents of Ixora coccinea and Hibiscus sabdarriffa plant species extracted using distilled water, methanol and chloroform. Approximately 150 g of each sample was soaked in the chosen solvent for four weeks, the percentage yields of the aqueous, methanol and chloroform extracts of Ixora coccinea were 38.47% and 13.40 % and 3.88 % respectively; Hibiscus sabdariffa gave yields of 22.85 % for the aqueous extract, 12.24 % for the methanol extract and 3.79 % for the chloroform extract. Aqueous, methanol and chloroform extracts of Ixora coccinea were all pink in colour; Hibiscus sabdariffa gave red aqueous extract, red methanol extract and orange chloroform extract. The extracts were used without further purification in dyeing unmordanted and mordanted cotton and polyester fabrics. Different colour shades were obtained after dyeing. The mordanted fabrics using CuSO4, FeSO4 and K2CrO7 were fast to acid, alkali and washing.Key words dyeing, extract, fastness, mordant, solvent
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Tomar, Oktay. "The effect of plant extracts on antioxidant potential, microbial and sensory attributes of stirred yoghurt." Mljekarstvo 71, no. 1 (December 14, 2020): 35–48. http://dx.doi.org/10.15567/mljekarstvo.2021.0104.
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The aim of this study was to use various plants to increase the functional properties of yoghurt. The ethanol extracts of three different plant (Mentha piperita L., Ocimum basilicum L., and Hibiscus sabdariffa L.) extracts were added to the stirred type yoghurts at different ratios (0.1 %, 0.3 % and 0.5 %). The pH values, colour values, antioxidant activity, microbial and sensory attributes of yoghurt were evaluated on the storage days of 0, 7, 14, 21, and 28. The lowest pH values were found at the beginning (4.29) and at the end (3.95) of the storage period in samples containing 0.5 % hibiscus extract. The addition of plant extracts in amounts of 0.3 % and higher decreased the lightness (L*) value of yoghurt (p<0.05), and the maximum decrease was found in yoghurt samples containing 0.5% hibiscus ethanol extract. The concentrations of added mint and basil extracts increased the yellowness (b*) value (p<0.05), while the addition of hibiscus extract significantly increased the redness value (p<0.05). Hibiscus ethanol extract significantly reduced the growth of total aerobic mesophilic bacteria (TAMB), lactic acid bacteria (LAB) and Lactococcus/Streptococcus bacteria (p<0.05). The antioxidant activity of all samples increased with adding plant extracts. According to the results of sensory analysis, the addition of 0.1 % plant extracts to yoghurts rated higher scores than that of the control sample, but the addition of 0.3 % and 0.5 % ethanol extracts negatively affected the sensory properties. It can be concluded that adding plant extracts had a positive effect on the sensory and functional properties of yoghurt.
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Baranova, V. S., I. F. Rusina, D. A. Guseva, N. N. Prozorovskaya, O. M. Ipatova, and O. T. Kasaikina. "The antiradical activity of plant extracts and healthful preventive combinations of these exrtacts with the phospholipid complex." Biomeditsinskaya Khimiya 58, no. 6 (2012): 712–26. http://dx.doi.org/10.18097/pbmc20125806712.
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Using the chemiluminescence method, the effective concentration of antioxidants (AO) and its reactivity toward peroxyl radicals (ARA, the k7 constant) have been measured for 13 plant extracts. In fact all extracts demonstrated ARA higher than ionol. Larix dahurica, Hypericum perforatum, Potentilla fruticosa, Aronia melanocarpa and Rhaponticum carthamoides extracts showed the highest values of ARA. The combinations Aronia + Raponticum extracts; Larix + Hibiscus extracts; Schizandra +Aronia extracts were synergistic (the synergism effect β of 38%, 33% and 22%). Apparently this phenomenon is the result of the synergistic interaction between compounds present in plant extracts. The Phospholipid complex - Lipoid S40, lacting any antioxidant effect alone, showed a potent synergistic effect with Aronia extract (β = 60%), Silybum extract (β = 41%). Clinical trials demonstrated, that combinations "Lipoid + Aronia extract", "Lipoid + Larix extract + Hibiscus extract", "Lipoid + Silybum extract", "Lipoid + Q10 + Rosa majalis extract" may be used as an additional component in the medicinal treatment, or as an individual prophylactic agent.
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Truong, Han, Ly Le, and Minh Tran. "Phytochemical Screening and Total Phenolics and Flavonoids Contents of Hibiscus Rosa Sinensis. L Cultivated in Viet Nam." MedPharmRes 1, no. 1 (December 10, 2017): 52–57. http://dx.doi.org/10.32895/ump.mpr.1.1.52/suffix.
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Background: Phenolics and flavonoids are regarded as the highest potential of chemotherapeutic activities. This investigation was carried out to evaluate phytochemical and total phenolics content (TPC) and total flavonoids content (TFC) and Fourier-transform infrared spectroscopy (FTIR) spectral analysis of Hibiscus Rosa Sinensis L. extracts (crude extract, n-hexane extract, ethyl acetate extract, and methanol extract) for further applications in pharmaceutical development. Method: Total phenolics were estimated by Folin-Ciocalteu method; while, Aluminum chloride was employed to quantify total flavonoids in the sample extracts. And, functional groups of Hibiscus Rosa Sinensis compound was determined using a FTIR-spectrophotometer. Result: Results showed the leaves extracts to encompass the high amount of total phenolic and total flavonoid content. TPC values for crude extract, methanol extract, ethyl acetate extract and n-hexane extract were 57.09 ± 0.35 mg/g, 70.98 ± 0.03 mg/g, 21.31 ± 0.01 mg/g, and 18.45 ± 0.003 mg/g as gallic acid equivalent, respectively. Crude extract, methanol extract, ethyl acetate extract and n-hexane extract showed total flavonoids 26.87 ± 0.01 mg/g, 21.08 ± 0.03 mg/g, 21.70 ± 0.001 mg/g, 14.95 ± 0.02 mg/g as rutin equivalent. FTIR spectra of four extracts were comparable and showed the presence of nitro compounds and ring aromatic compounds. Conclusion: Our results indicate the potential of exploiting Hibiscus Rosa Sinensis leaves as a source of chemotherapeutic compounds, and it is worthy doing further researches on isolated bioactive compounds for developing novel functional foods or new drugs.
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Al-Yousef, Hanan M., Wafaa H. B. Hassan, Sahar Abdelaziz, Musarat Amina, Rasha Adel, and May A. El-Sayed. "UPLC-ESI-MS/MS Profile and Antioxidant, Cytotoxic, Antidiabetic, and Antiobesity Activities of the Aqueous Extracts of Three Different Hibiscus Species." Journal of Chemistry 2020 (June 25, 2020): 1–17. http://dx.doi.org/10.1155/2020/6749176.
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The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50 = 50 ± 5.1 μg/mL, and Hibiscus micranthus showed a close effect with IC50 = 60.4 ± 1.7 μg/mL. Hibiscus micranthus showed the most potent effect on HCT-116 (human colon carcinoma) with IC50 = 56 ± 1.9 μg/mL compared with cisplatin (IC50 = 7.53 ± 3.8 μg/mL). HcA and HdA extracts showed weak cytotoxic activity against A-549 and HCT-116 cell lines compared to the other extracts. Eventually, Hibiscus deflersii showed astonishing antidiabetic (IC50 = 56 ± 1.9 μg/mL) and antiobesity (IC50 = 95.45 ± 1.9 μg/mL) activities using in vitro α-amylase inhibitory assay (compared with acarbose (IC50 = 34.71 ± 0.7 μg/mL)) and pancreatic lipase inhibitory assay (compared with orlistat (IC50 = 23.8 ± 0.7 μg/mL)), respectively. In conclusion, these findings are regarded as the first vision of the phytochemical constituents and biological activities of different Hibiscus aqueous extracts. Hibiscus deflersii aqueous extract might be a hopeful origin of functional constituents with anticancer (on A-549 cell line), antidiabetic, and antiobesity activities. It might be a natural alternative remedy and nutritional policy for diabetes and obesity treatment without negative side effects. Isolation of the bioactive phytochemicals from the aqueous extracts of aerial parts of Hibiscus calyphyllus, Hibiscus micranthus, and Hibiscus deflersii and estimation of their biological effects are recommended in further studies.
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Portillo-Torres, Lizbeth Anahí, Aurea Bernardino-Nicanor, Carlos Alberto Gómez-Aldapa, Simplicio González-Montiel, Esmeralda Rangel-Vargas, José Roberto Villagómez-Ibarra, Leopoldo González-Cruz, Humberto Cortés-López, and Javier Castro-Rosas. "Hibiscus Acid and Chromatographic Fractions from Hibiscus Sabdariffa Calyces: Antimicrobial Activity against Multidrug-Resistant Pathogenic Bacteria." Antibiotics 8, no. 4 (November 11, 2019): 218. http://dx.doi.org/10.3390/antibiotics8040218.
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The anti-microbial properties of acetone extracts from Hibiscus sabdariffa calyces, fractions isolated by silica gel chromatography and hibiscus acid purified from some of these fractions and additionally identified by nuclear magnetic resonance spectroscopy, mid-infrared spectroscopy and X-ray diffraction, were studied against both multidrug-resistant Salmonella strains and pathogenic Escherichia coli bacteria. Gel diffusion was used to determine the anti-microbial effects. The mode of action of hibiscus acid was determined by crystal violet assay. Hibiscus acid and 17 of the 25 chromatographic fractions obtained, displayed an anti-microbial effect against all bacterial strains tested. Hibiscus acid showed a greater anti-microbial effect than the acetone extract against most of the bacteria strains, while chromatographic fractions IX–XIV exerted the greatest anti-microbial effect against all bacteria. The minimum inhibitory concentration of the acetone extract was 7 mg/mL, and the minimum bactericidal concentration was 10 mg/mL, while the corresponding values for hibiscus acid were 4–7 and 7 mg/mL, respectively. The results of the crystal violet assay indicate that hibiscus acid alters membrane permeability. Hibiscus acid is a potential alternative to control multidrug-resistant bacteria. Due to its ready availability and easy extraction from H. sabdariffa, hibiscus acid is potentially useful in the food industries.
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Okoduwa, Stanley I. R., Lovina O. Mbora, Matthew E. Adu, and Ameh A. Adeyi. "Comparative Analysis of the Properties of Acid-Base Indicator of Rose (Rosa setigera), Allamanda (Allamanda cathartica), and Hibiscus (Hibiscus rosa-sinensis) Flowers." Biochemistry Research International 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/381721.
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The need to develop effective alternative for synthetic indicators is the demand of present-day chemistry. The acid-base indicator properties of Rose (Rosa setigera), Allamanda (Allamanda cathartica), and Hibiscus (Hibiscus rosa-sinensis) flowers were examined. Colour pigments were extracted from the flowers via cold and solvent extraction using soxhlet extractor. The pH value of the extracts with wavelengths of absorption was determined using ultraviolet spectrophotometer. From the results obtained, all the extracts exhibited sharp contrast between their colours in acid and base. Their pH was found to be 5.5 for cold extract of Rose and 5.6 for solvent extraction, 5.24 for cold extract of a Hibiscus and 6.52 for solvent extraction, 5.35 for cold extract of Allamanda, and 5.45 for solvent extraction. The maximum wavelengths of absorption obtained for all the extract fall within the visible region of electromagnetic spectrum. These values are almost similar to that obtained from synthetic indicators. It is on these bases that we concluded that natural indicators could be an excellent replacement for synthetic indicators since they are cheap, readily available, simple to extract, not toxic, user and environmentally friendly.
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Firmasari, Firmasari, Ni Ketut Sumarni, Ruslan, and Hardi Ys. "PERSEN DEGRADASI EKSTRAK ETANOL KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) SELAMA PENYIMPANAN THE PERCENTAGE OF ETHANOL EXTRACTS OF ROSELLE PETALS (Hibiscus sabdariffa L.) DURING STORAGE." KOVALEN: Jurnal Riset Kimia 5, no. 2 (August 31, 2019): 132–38. http://dx.doi.org/10.22487/kovalen.2019.v5.i2.9970.
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Ethanol extract of roselle petals (Hibiscus sabdariffa L.) is more stable in low pH than in high pH. The research aims to monitor and determine the effect of ratio content in ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce and the pH effect on the percentage of ethanol extract degradation during storage. Two independent variables implemented in this study, are ratio content of ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce and pH. Ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce with ratio content 0,5:1; 1:1; and 1:0 (w/w) and adjusted to pH 2, pH 3, pH 4, pH 5 and pH 6, respectively were investigated. The absorbance measurement of Ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce was depend on each treatment, then analyzed using completely randomized design. The percentage of ethanol extract of roselle petals (Hibiscus sabdariffa L.) degradation in tomato sauce decreased in ratio content 1:0 (w/w) at pH 2 with percentage content of degradation is 30.09%. Furthermore, after 10 days of storage, the highest percentage of ethanol extract of roselle petals (Hibiscus sabdariffa L.) in tomato sauce degradation is 93.33% with ratio content is 0.5:1 at pH 6 and the lowest is 30.09% with ratio content 1:0 at pH 2.
Keywords: Roselle petals, Ethanol Extract, Tomato Sauce, Percentage of Degradation
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Cahlíková, Lucie, Badreldin H. Ali, Lucie Havlíková, Mirek Ločárek, Tomáš Siatka, Lubomír Opletal, and Gerald Blunden. "Anthocyanins of Hibiscus sabdiffera Calyces from Sudan." Natural Product Communications 10, no. 1 (January 2015): 1934578X1501000. http://dx.doi.org/10.1177/1934578x1501000120.
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Extracts of the calyces of Hibiscus sabdariffa are widely used in folk medicine to combat many illnesses. The active constituents of the extracts have been shown on several occasions to be anthocyanins. In our current studies the biological activities of an extract of H. sabdariffa calyces purchased in Oman, but grown in Sudan, are being compared with those of the anthocyanins isolated from them, and, for this, the anthocyanin profile of the extract needed to be ascertained. Although several anthocyanins were detected by UHPLC-ESI-MS/MS, delphinidin-3-sambubioside (major) and cyanidin-3-sambubioside were predominant.
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Petta, Nabila S., Edwin De Queljoe, and Rooije R. H. Rumende. "PENGARUH PEMBERIAN EKSTRAK ETANOL KEMBANG SEPATU (Hibiscus rosa-sinensis L.) TERHADAP JUMLAH SPERMATOZOA, BERAT BADAN, DAN BERAT TESTIS TIKUS JANTAN WISTAR (Rattus norvegicus)." JURNAL ILMIAH SAINS 19, no. 1 (January 24, 2019): 6. http://dx.doi.org/10.35799/jis.19.1.2019.21678.
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PENGARUH PEMBERIAN EKSTRAK ETANOL KEMBANG SEPATU (Hibiscus rosa-sinensis L.) TERHADAP JUMLAH SPERMATOZOA, BERAT BADAN, DAN BERAT TESTIS TIKUS JANTAN WISTAR (Rattus norvegicus)ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol kembang sepatu terhadap jumlah spermatozoa tikus jantan wistar (Rattus norvegicus). Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan menggunakan 24 ekor tikus putih jantan galur wistar (Rattus norvegicus) yang dibagi atas beberapa kelompok dimana kelompok 1 sebagai kelompok kontrol tanpa perlakuan, kelompok 2, 3 dan 4 sebagai kelompok perlakuan dengan dosis secara berturut-turut 3,6 mg/ml; 7,2 mg/ml; dan 14,4 mg/ml. Perlakuan diberikan secara oral sekali sehari sebanyak 1 ml selama 50 hari sesuai siklus spermatogenesis. Variabel yang diamati yakni jumlah sel spermatozoa, berat badan, dan berat testis. Hasil penelitian ini menunjukkan bahwa ekstrak etanol kembang sepatu dapat menurunkan jumlah sel spermatozoa, serta menyebabkan adanya perbedaan berat badan dan berat testis namun, berdasarkan hasil analisis varians, ekstrak etanol daun kembang sepatu tidak dapat menurunkan jumlah sel spermatozoa, berat badan dan berat testis tikus putih jantan galur wistar (Rattus norvegicus) secara signifikan.Kata Kunci: Sel spermatozoa, Kembang sepatu (Hibiscus rosa-sinensis L.), Tikus jantan Wistar (Rattus novergicus) THE INFLUENCE OF THE ETHANOL EXTRACTS OF GRANTING HIBISCUS (Hibiscus Rosa-sinensis L.) AGAINST THE NUMBER OF SPERMATOZOA, WEIGHT AND THE WEIGHT OF THE TESTES MALE WISTAR RATS (Rattus norvegicus) ABSTRACTThis research’s objective is to know the influence of injecting ethanol extract from a hibiscus into a number of common male rats (Rattus norvegicus). This research uses the approach of complete randomized design (CRD) onto 24 common white rats (Rattus norvegicus) that is divided into groups, where group 1’s approach is control without treatment, groups 2, 3, and 4’s approach is with treatment, with consecutive doses being 3.6 mg/ml; 7.2 mg/ml; and 14.4 mg/ml. The treatment is induced orally as large as 1cc per day for a total of 50 days following the spermatogenesis cycle. The variables that are being observed are the amount of spermatozoon cells, body weight, and testicle weight. The results of this research indicates that ethanol extract from hibiscuses, from a quantity perspective, can decrease spermatozoon cells, and it may also influence the body weight and testicle weight of the subject, in this case are common rats (Rattus norvegicus) but, from the mathematical results from Analysis Of Variance, ethanol extract from the leaves of a hibiscus cannot decrease the amount of spermatozoon, body weight, and testicle weight of a common white rat (Rattus norvegicus).Keywords: Spermatozoon Cells, Hibiscus (Hibiscus rosa-sinensis L.), Common (white) rat (Rattus novergicus)
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Tazoho, Ghislain Maffo, Esther Etengeneng Agbor, and Inocent Gouado. "In vitro, antioxidant activities of aqueous and methanol roselle (Hibiscus sabdariffa) calyces extracts from two localities in Cameroon." North African Journal of Food and Nutrition Research 4, no. 8 (September 23, 2020): 292–97. http://dx.doi.org/10.51745/najfnr.4.8.292-297.
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Background: Fighting against malnutrition and diseases such as oxidative stress diseases via a food-based approach could be achieved through identification, valorization, and promotion of local foods rich in macro- and micronutrients and phytochemical components. Aim: This study aimed to investigate the effect of agro-ecological conditions on the antioxidant capacity of the Hibiscus sabdariffa aqueous and methanol calyces extracts. Material and methods: The total phenolic content, the free radical DPPH• (1,1-diphenyl-2-picrylhydrazyl), and ferric reducing antioxidant power (FRAP) activities were evaluated in aqueous and methanol Hibiscus sabdariffa calyces extract samples from two localities (Dschang [western highland zone] and Ngaoundéré [high Guinean savanna zone]) in Cameroon. Results: The results obtained showed that these extracts contain an important amount of total phenolic compounds with no significant difference (P>0.05) between aqueous and methanol extracts and also between the origins of calyces. The extracts showed their ability to reduce Fe3+ to Fe2+ as well as their ability to reduce the free radical, DPPH•. Concerning the FRAP results, at the concentration of 12.5µg/mL, the absorbance of AEN (Aqueous Extract from Ngaoundéré locality) recorded the value (0.33±0.05) meanwhile at a far higher concentration of 200 µg/mL, the absorbance of MEN (Methanol Extract from Ngaoundéré locality) was the highest (1.39±0.06). The CI50 of methanol extracts was significantly low (P<0.05) compared to that of aqueous extracts. The value ranges were 11.31±0.15, 14.69±0.84, 18.07±0.63, 20.50±0.54, and 21.50±0.54 µg/mL for ascorbic acid, MEN (Methanol Extract from Ngaoundéré locality), MED (Methanol Extract from Dschang locality), AEN (Aqueous Extract from Ngaoundéré locality), and AED (Aqueous Extract from Dschang locality) respectively. Conclusion: These findings show that the antioxidant activity of methanol extract is higher than that of aqueous extract. Hibiscus calyces from the two localities could be used as a natural antioxidant in the treatment of oxidative stress diseases. Keywords: Roselle calyces, aqueous and methanol extracts, antioxidant capacity.
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Maffo Tazoho, Ghislain, Esther Etengeneng Agbor, and Inocent Gouado. "In vitro, antioxidant activities of aqueous and methanol roselle (Hibiscus sabdariffa) calyces extracts from two localities in Cameroon." North African Journal of Food and Nutrition Research 4, no. 08 (September 23, 2020): 292–97. http://dx.doi.org/10.51745/najfnr.4.08.292-297.
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Background: Fighting against malnutrition and diseases such as oxidative stress diseases via a food-based approach could be achieved through identification, valorization, and promotion of local foods rich in macro- and micronutrients and phytochemical components. Aim: This study aimed to investigate the effect of agro-ecological conditions on the antioxidant capacity of the Hibiscus sabdariffa aqueous and methanol calyces extracts. Material and methods: The total phenolic content, the free radical DPPH• (1,1-diphenyl-2-picrylhydrazyl), and ferric reducing antioxidant power (FRAP) activities were evaluated in aqueous and methanol Hibiscus sabdariffa calyces extract samples from two localities (Dschang [western highland zone] and Ngaoundéré [high Guinean savanna zone]) in Cameroon. Results: The results obtained showed that these extracts contain an important amount of total phenolic compounds with no significant difference (P>0.05) between aqueous and methanol extracts and also between the origins of calyces. The extracts showed their ability to reduce Fe3+ to Fe2+ as well as their ability to reduce the free radical, DPPH•. Concerning the FRAP results, at the concentration of 12.5µg/mL, the absorbance of AEN (Aqueous Extract from Ngaoundéré locality) recorded the value (0.33±0.05) meanwhile at a far higher concentration of 200 µg/mL, the absorbance of MEN (Methanol Extract from Ngaoundéré locality) was the highest (1.39±0.06). The CI50 of methanol extracts was significantly low (P<0.05) compared to that of aqueous extracts. The value ranges were 11.31±0.15, 14.69±0.84, 18.07±0.63, 20.50±0.54, and 21.50±0.54 µg/mL for ascorbic acid, MEN (Methanol Extract from Ngaoundéré locality), MED (Methanol Extract from Dschang locality), AEN (Aqueous Extract from Ngaoundéré locality), and AED (Aqueous Extract from Dschang locality) respectively. Conclusion: These findings show that the antioxidant activity of methanol extract is higher than that of aqueous extract. Hibiscus calyces from the two localities could be used as a natural antioxidant in the treatment of oxidative stress diseases. Keywords: Roselle calyces, aqueous and methanol extracts, antioxidant capacity.
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Dafallah, Abdullah A., and Zaki Al-Mustafa. "Investigation of the Anti-inflammatory Activity of Acacia nilotica and Hibiscus sabdariffa." American Journal of Chinese Medicine 24, no. 03n04 (January 1996): 263–69. http://dx.doi.org/10.1142/s0192415x96000323.
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The aqueous extracts of Acacia nilotica and Hibiscus sabdariffa were tested for anti-inflammatory, analgesic and antipyretic activities in animal models. Acacia nilotica extract had an inhibitory effect on carrageenan induced paw edema and yeast-induced pyrexia in rats. It also produced a significant increase in the hot plate reaction time in mice. Hibiscus sabdariffa extract had no effect on paw edema but had an inhibitory effect on yeast induced pyrexia and a significant effect on the hot plate reaction time. Among the phytoconstituents found in both plants, flavanoids, polysaccharides and organic acids may be mainly responsible for their pharmacological activities.
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Verma, Pankaj, Swati Sharma, Vikas Kumar, and Hema Chaudhary. "Anti-inflammatory effect of hydroalcoholic extract of hibiscus rosa on acute and chronic inflammation." International Journal of Pharmacology and Toxicology 4, no. 2 (June 28, 2016): 123. http://dx.doi.org/10.14419/ijpt.v4i2.6230.
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Background: The present study was carried out to explore the efficiency of Indian herbal source from Hibiscus rosa against a chronic inflammatory disease. Hibiscus rosa belongs to Malvaceae, acts by suppression of inflammation mediators.Methods: Hydroalcoholic extract form Hibiscus rosa is prepared through soxhlet extraction and Diclofenac is used as the standard. Carrageenan and formaldehyde are administered to induce acute and chronic inflammation. Animals are divided into 6 groups with 6 animals each including Normal group, inflammatory control group, Diclofenac treated group and Hibiscus rosa treated group at different doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg.Results: Different concentrations of Hibiscus rosa treated groups i.e. 250 mg/kg (p<0.05), 500 mg/kg (p<0.05) and 1000 mg/kg (p<0.01) showed significant reduction in Paw edema as compared to controls. Significant reduction in Body weight was also observed in Hibiscus rosa treated groups. Hematological profiles of Hibiscus rosa treated group are satisfying and significant.Conclusion: Results showed significant anti-inflammatory effect of Hydroalcoholic extract of Hibiscus rosa and justifying its therapeutic role in inflammatory condition..
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Ngozi, Nwankwo Rita, Ocheola-Oki Joyce Ene, and Monsurat Moshod Tunrayo. "Phytochemical, Antinutrients, Food Toxicants Analysis and Comparative Efficacy of Methanol Extracts of Selected Vegetables of Adamawa State Nigeria on the Haematological Parameters of Alloxan Induced Diabetic Rats." Journal of Food Sciences 2, no. 1 (August 13, 2021): 1–23. http://dx.doi.org/10.47941/jfs.518.
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Purpose: Phytochemicals present in vegetables has been associated with the health potentials of leafy vegetables. Diabetes has been implicated with increased oxidative stress leading to alteration of haematological parameters among other complications. This study investigated the phytochemicals, antinutrients and toxicants contents of methanol extracts of Hibiscus cannabinus, Adansonia digitata, Sesamum indicum and Cassia tora leaves and compared the effect of their leaves extract on some haematological parameters of alloxan – induced diabetic rats using standard methods of analysis.Methodology: Fifty-five (55) male albino rats weighing 120 – 150g divided into 11 groups of five rats each were used. One diabetic untreated rat group, one diabetic group treated with standard drug ,8 diabetic groups treated with graded doses of the vegetable extracts and one normal group as control. All the groups received water and feed ad libitum together with their various treatments for 23 days. At the end of the experiment, blood was taken from the rats for determination of PCV, Hb, WBC, and RBC. ANOVA was used to separate the means of all the data collected with significance at p< 0.05 using Statistical Package for Scientist and Engineers (SPSE) version 9.1.Results: Results showed reasonable levels of phytochemicals, antinutrient and toxicants levels within WHO safe limits. Results of animal studies showed that administration of 500mg of Hibiscus cannabinus leaf extract increased the PCV, HB, WBC and RBC of diabetic rats more than all the other vegetable extracts (p<0.05). It also improved the WBC and RBC better than standard drug. This shows that Hibiscus cannabinus leaf extract might improve some haematological parameters associated with diabetes mellitus, however its inhibitory effect on higher dose raised concern for further investigation.Unique contribution to theory, practice and policy: Hibiscus cannabinus leaf extract could be used in the dietary management of haematological abnormalities associated with the pathophysiology of diabetes mellitus by health care systems
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Alekhya V, Ganapaty S, and Deepan T. "Phytochemical and Pharmacological evaluation of hibiscus hispidissimus griff." International Journal of Research in Pharmaceutical Sciences 11, SPL4 (December 21, 2020): 2063–67. http://dx.doi.org/10.26452/ijrps.v11ispl4.4420.
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To assess phytochemical with pharmacological studies of Hibiscus hispidissimus griff belong to family malavaceae. Preliminary phytochemical analysis reveals the presence of steroids, triterpenes, saponins, steroidal saponins and phenols. Evaluation of anti-inflammatory, anti-microbial with antioxidant action were performed on aerial parts of methanolic extract of Hibiscus hispidissimus. Invitro antioxidant activity was performed by 2, 2 -diphenyl- 1- picrylhydrazyl (DPPH) assay, hydroxy radical scavenging method and superoxide radical scavenging activity.The results of invitro antioxidant study reveal that % inhibition of H. hispidissimus was higher compared to ascorbic acid. Anti-inflammatory studies were performed using carrageenan-induced rat paw oedema animal model, for anti-inflammatory studies, the extracts were compared with standards like indomethacin, and it shows a remarkable zone of inhibition ranging from 58.97 to 71.73 respectively. The anti-bacterial and antifungal activity of plant extracts were studied for the occurrence of inhibition zones. The activity was performed by the cup plate method. Ethanolic extract of H. Hispidissimus shows significant anti-bacterial effect against S. Aureus, B. Subtilis, P. Vulgaris and E. coli using ciprofloxacin (50µg/ml) as standard.The extracts show remarkable inhibition of zone of inhibition, and results were compared with that of standard drugs against the organism tested. In conclusion, the ethanolic extract of H. hispidissimus shows significant antioxidant, anti-inflammatory and anti-bacterial properties.
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Navidad-Murrieta, Migdalia Sarahy, Alejandro Pérez-Larios, Jorge Alberto Sanchéz-Burgos, Juan Arturo Ragazzo-Sánchez, Gabriel Luna-Bárcenas, and Sonia G. Sáyago-Ayerdi. "Use of a Taguchi Design in Hibiscus sabdariffa Extracts Encapsulated by Spray-Drying." Foods 9, no. 2 (January 24, 2020): 128. http://dx.doi.org/10.3390/foods9020128.
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Aqueous and ethanolic extracts of Hibiscus sabdariffa were spray-dried using maltodextrin (MD) and gum arabic (GA) as carrier agents. A Taguchi L8 experimental design with seven variables was implemented. Physicochemical properties in the encapsulates were evaluated by Ultraviolet-Visible (UV-Vis,) X-ray Diffraction (XRD), spectroscopy and gravimetric techniques. Treatments with aqueous extracts showed the highest concentration of total soluble polyphenols (TSP) 32.12–21.23 mg equivalent gallic acid (EAG)/g dry weight (DW), and antioxidant capacity (AOX) in the 2,2-azinobis-3-ethylbenzotiazoline-6-sulfonic acid (ABTS) assay. The best treatment for TSP and AOX was T4: 2.5% Hibiscus w/w, aqueous extract, decoction, extract-to-carrier ratio 1:1 (w/w), proportion to carriers (MD:GA) 80:20 (w/w), 10,000 rpm, 150 °C. The Taguchi L8 design is a tool that allows the use of multiple variables with a low number of treatments that indicate the drying conditions that give the best parameters, focusing mainly on TSP and AOX, also, it is a good alternative for the preservation and stability of the phenolic compoudns in Hibiscus.
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Webber III, Charles L., Paul M. White Jr, Dwight L. Myers, James W. Shrefler, and Merritt J. Taylor. "Kenaf (Hibiscus cannabinus L.) Impact on Post-Germination Seedling Growth." Journal of Agricultural Science 7, no. 12 (November 8, 2015): 91. http://dx.doi.org/10.5539/jas.v7n12p91.
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<p>The chemical interaction between plants, which is referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the impact of kenaf (<em>Hibiscus cannabinus</em> L.) plant extracts on the post-germination growth of five plant species. Four concentrations (0, 16.7, 33.3 and 66.7 g/L) of kenaf bark, core, and leaf extracts were applied to the germinated seeds of redroot pigweed (<em>Amaranthus retroflexus</em> L.), green bean (<em>Phaseolus vulgaris</em> L.), tomato (<em>Solanum lycopersicum </em>Mill.), cucumber (<em>Cucumis sativus</em> L.), and Italian ryegrass (<em>Lolium multiflorum</em> Lam.). After 7 days, the developing seedlings were measured to determine the length of their hypocotyls (mm) and radicles (mm), and the number of hair roots. Tomato, Italian ryegrass, and redroot pigweed followed similar negative trends in their responses to the extract source (kenaf bark, core, and leaves) and the impact of extract concentration, whereas, cucumber had a mixed response and green bean reacted positively to the kenaf extracts. Tomato was the most sensitive species tested across all kenaf extracts and concentrations, resulting in decreased hypocotyl, radicle, and root growth. Green bean exhibited no negative effects due to the kenaf extracts, but actually produced increased hypocotyl growth as a result of the kenaf bark, core, and leaf extracts. The kenaf extracts resulted in a mixed response for cucumber. The kenaf leaf and bark extract decreased cucumber radicle growth, whereas, the bark and core extracts increased hypocotyl growth. Italian ryegrass hypocotyl growth decreased across all extract sources (bark, core, and leaf), while the leaf extract also reduced root growth. All kenaf extracts reduced redroot pigweed radicle growth, while the core and leaf extracts reduced hypocotyl growth. The research demonstrated that kenaf leaf extracts were the most allelopathic and the hypocotyls were the most sensitive. Future research should isolate the chemicals responsible for both the negative and positive allelopathic impact on the various plant species, determine if the extracts will influence more mature plants, and pursue cultural practices to utilize these natural allelopathic materials to benefit crop production and limit weed competition.</p>
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Purnama, Rio. "EFEKTIVITAS PENGGUNAAN EKSTRAK ANTOSIANIN TANAMAN BUNGA KEMBANG SEPATU UNTUK MENDETEKSI BORAKS PADA BAKSO." Jurnal Delima Harapan 8, no. 2 (August 30, 2021): 21–25. http://dx.doi.org/10.31935/delima.v8i2.126.
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One of the causes of food safety problems in Indonesia is the lack of knowledge, responsibility and supervision by producers, consumers and the authorities. This results in cheating by irresponsible food producers, adding ingredients that can harm the human body. There are several ways to detect or detect the presence of hazardous food additives, one of which is using indicators made from natural ingredients added with several other ingredients that can detect hazardous food additives, one of which is borax. After conducting research on the effectiveness of hibiscus flower anthocyanin extract as an indicator of borax detection in meatballs, it was found that hibiscus flower extract can be used as an indicator for borax detection, seen from the change in the color of hibiscus flower extract which at first is brownish orange (purplish. ) After being absorbed with meatballs containing borax, the hibiscus flower extract changes its color to dark brown red, this is due to the pelagornidin antasianin compounds reacting with borax compounds.
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Olaokun, Oyinlola O., and Nqobile M. Mkolo. "Biological activity of infusion and decoction extracts of Hibiscus sabdariffa L. calyces." Bangladesh Journal of Botany 49, no. 1 (March 31, 2020): 171–77. http://dx.doi.org/10.3329/bjb.v49i1.49127.
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To compare the phytochemical and biological profiles, infusion and decoction extracts of dried Hibiscus sabdariffa calyces were prepared. Infusion extract had the highest phenolic content (292.42 ± 0.62 mg GAE/g extract) and antioxidant activity (TEAC = 3.09 ± 0.28). It also potently inhibited the activities of α-amylase (58.57 ± 1.4%), α-glucosidase (66.92 ± 2.5%) and 5-lipoxygenase (58.97 ± 1.17 μg/ml). All extracts showed weak cytotoxicity as tested on Vere monkey kidney cells.
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Piovesana, Alessandra, and Caciano P. Zapata Noreña. "Study of Acidified Aqueous Extraction of Phenolic Compounds from Hibiscus sabdariffa L. calyces." Open Food Science Journal 11, no. 1 (February 28, 2019): 25–34. http://dx.doi.org/10.2174/1874256401911010025.
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Introduction:Hibiscus calyces are important sources from anthocyanins and pigments. The recovery of these bioactive compounds using non-organic solvents becomes very attractive for the food industry.Methods:For this reason, the separation of phenolic compounds by acidified aqueous extraction from hibiscus calyces was studied. The experiments were conducted by a fractional factorial design.Result and Conclusion:Four factors were evaluated: temperature, time, stirring speed and enzyme concentration. The extracts produced were subjected to analysis of color (L*,a*,b*andChroma), total monomeric anthocyanins, antioxidant capacity by ABTS and fourteen phenolic compounds were quantified. The results showed that the best condition to obtain hibiscus calyces extract was using an enzyme concentration of 50 µL/1000 g hibiscus extract, 400 rpm of stirring speed at 55 ºC by 4 hours of extraction, that corresponded to concentrations of 17595, 7516, 2568 μg/g, expressed on a dry basis, for total phenolic compounds, delphinidin 3-sambubioside and cyanidin 3-sambubioside, respectively, and antioxidant capacity measured by ABTS of 7.8 µmol of Trolox equivalent per gram.
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Arogbodo, Joseph Olowo, Oyetayo Bolanle Faluyi, and Festus Omotere Igbe. "In vitro Antimicrobial Activity of Ethanolic Leaf Extracts of Hibiscus Asper Hook. F. and Hibiscus Sabdariffa L. on some Pathogenic Bacteria." Journal of Scientific Research in Medical and Biological Sciences 2, no. 3 (August 12, 2021): 1–12. http://dx.doi.org/10.47631/jsrmbs.v2i3.304.
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Purpose: The study aims to assess the antimicrobial activity of ethanolic leaf extracts of Hibiscus asper and Hibiscus sabdariffa against eight bacterial isolates. Materials and Methods: An in vitro Antimicrobial activity of ethanolic leaf extract of the two plants against eight nosocomical and pathogenic bacteria viz; Pseudomonas aeruginosa (PAE), Proteus vulgaris (PVU), Klebsiella aerogenes (KAE), Staphylococcus aureus (SAU), Bacillus cereus (BCE), Escherichia coli (ECO), Moraxella catarrhalis (MCA) and Salmonella typhi (STY) was carried out using agar well diffusion assay with the concentration range of 3.13 – 100 mg/mL. Results: H. asper and H. sabdariffa showed significant difference (p< 0.05) in antimicrobial activity against BCE over the rest of the isolates. Inhibition zone diameters exhibited by the isolates to ethanolic leaf extract of H. asper was in descending order of BCE (15.00 ± 1.00a) >ECO (11.67 ± 0.58b) >SAU (7.67 ± 0.58c) >PAE (6.67 ± 0.58d) >STY (5.67 ± 0.58e) while that of H. sabdariffa was in the order BCE (15.33 ± 1.15a) > MCA (11.33 ± 1.15b) > SAU (11.00 ± 1.00bc) > KAE (9.67 ± 0.58c) > PAE (8.00 ± 1.00d) >PVU (7.67 ± 0.57e). PVU, KAE and MCA were resistant to the extract of H. asper while only STY was resistant to that of H. sabdariffa. Conclusion: H. sabdariffa extract demonstrated higher antimicrobial activity against the selected bacterial isolates than H. asper. However, the two extracts minimum inhibition concentrations (MICs) ranged from 25 mg/mL to 12.5 mg/mL. This is worthy of further exploration by pharmacological industries in the formulation of potent broad spectrum antibiotics for combating the present health challenge due to antimicrobial resistance.
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Amos, S., L. Binda, B. A. Chindo, A. Tseja, A. A. Odutola, C. Wambebe, and K. Gamaniel. "Neuropharmacological Effects of Hibiscus sabdariffa Aqueous Extract." Pharmaceutical Biology 41, no. 5 (January 2003): 325–29. http://dx.doi.org/10.1076/phbi.41.5.325.15933.
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Ali, Özmen. "Cytotoxicity of Hibiscus rosa-sinensis flower extract." Caryologia 63, no. 2 (April 2010): 157–61. http://dx.doi.org/10.1080/00087114.2010.10589721.
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Adefolalu, F. S., J. S. Salawa, T. Y. Gara, and A. N. Abubakar. "Hypoglycemic and Hypolipidemic Effect of Methanol Extract of Hibiscus Sabdariffa Seed in Alloxan Induced Diabetic Albino Rats." Nigerian Journal of Basic and Applied Sciences 27, no. 2 (May 27, 2020): 151–56. http://dx.doi.org/10.4314/njbas.v27i2.20.
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This study investigated the hypoglycemic and hypolipidemic effect of the oral administration of methanol extract of Hibiscus sabdariffa seed in alloxan induced diabetic albino rats. Two different doses (200 and 400 mg/kg) of the methanol extract of Hibiscus sabdariffa seed were administered daily to two groups of diabetic rats for a period of two weeks. Metformin (250 mg/kg) was given as the reference standard drug. Phytochemical, acute toxicity, fasting blood glucose, body weight, cholesterol, triglycerides and lipoproteins levels were determined using standard methods. Acute toxicity of methanol extract of Hibiscus sabdariffa seed was estimated to be greater than 5000 mg/kg body weight. There was an initial decrease in the body weight of the experimental animals after induction of diabetes which increased significantly (p<0.05) by the second week of treatment. The fasting blood glucose levels of the treated diabetic rats were significantly decreased at the second week of treatment with the group that received 400 mg/kg of extract recording the lowest fasting blood glucose level. The cholesterol, triglycerides and low density lipoproteins levels of the diabetic treated rats were significantly reduced while the high density lipoproteins level significantly increased. It can therefore be concluded that Hibiscus sabdariffa seed has the ability to lower blood sugar and maintain a stable lipid level.
Keywords: Hypoglycemic, hypolipidemic, Alloxan, Phytochemical, Hibiscus sabdariffa
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Araújo, Auryclennedy Calou de, Francislaine Suelia dos Santos, Marylia de Sousa Costa, Karoline Thays Andrade de Araújo, Josivanda Palmeira Gomes, Wilton Pereira da Silva, Alexandre José de Melo Queiroz, Geovani Soares de Lima, Lauriane Almeida dos Anjos Soares, and Ângela Maria Santiago. "Thermal degradation kinetics and physicochemical analysis of the freeze-dried hibiscus flower extract (Hibiscus sabdariffa)." March 2021, no. 15(03):2021 (March 5, 2021): 325–33. http://dx.doi.org/10.21475/ajcs.21.15.03.p2503.
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The goal of this research was to evaluate the kinetics of thermal degradation of the hibiscus extracts powder at different temperatures and to characterize physicochemically the freeze-dried aqueous extract of the hibiscus flower (Hibiscus sabdariffa) with different concentrations of maltodextrin (0, 5, 10 and 15%). The analyzed parameters were: water content, total soluble solids, titratable acidity, pH, ash, solubility, hygroscopicity, bulk and tapped density, the flowability (Carr index) and cohesiveness (Hausner ratio), lightness (L*) and intensity of red (a*) and yellow (b*). The degradation kinetics was analyzed by the determination of bioactive compounds of flavonoids, anthocyanins and color appearance parameters (lightness, intensity of red and yellow, chroma and hue angle). The increase in maltodextrin concentration resulted in increases of solubility, soluble solids content, pH and color appearance parameters; as the parameters of bulk and tapped density, hygroscopicity, water content, ash and acidity presented decreases as the proportion of additive increased, showing good flowability and low to medium cohesion. Regarding the degradation kinetics, the maltodextrin was effective in the preservation of anthocyanins and flavonoids of the freeze-dried hibiscus flower up to the temperature of 50 °C. Therefore, this work resulted in the preparation of a product derived from the freeze-drying process with and without additives and with preserved sensory attributes of high quality
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Mulyani, Sri, Kris Siska Aristia, Ardia Putri Sabrina, Afifatul Arfiah, and Badrun Niam. "Potential of Water Extract of The White Frangipani (Plumeria acuminate) and Hibiscus (Hibiscus tiliaceus) Leaves Powder as Textile Natural Dyes." JKPK (Jurnal Kimia dan Pendidikan Kimia) 5, no. 1 (April 30, 2020): 100. http://dx.doi.org/10.20961/jkpk.v5i1.25810.
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<p class="Default">Synthetic dyes have a high environmental impact, especially on waters. The use of natural dyes for textiles provides new hope for ecological improvement. This article is to find out the yield of water extract of white frangipani (<em>Plumeria acuminate</em>) and hibiscus (<em>Hibiscus tiliaceus</em>) leaves-powder, as well as to find out whether the water extracts have potential as textile natural dyes. Natural dyes must have a good quality of fastness. For this reason, the color fastness tests for washing with a launder meter and colorfastness against rubbing with a crock meter are performed. Colorfastness is assessed by comparing color changes to washing with grayscale and color changes due to staining on fabric with staining scale. The rubbing test is carried out on the wet and dry cloth. Cotton and polyester fabrics are samples that will be colored. Fixers used to bind dyes are Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, CaCO<sub>3</sub>, and FeSO<sub>4</sub>.H<sub>2</sub>O. The results show that the water extraction of hibiscus and white frangipani leaves powder gave a yield of 11% (w/w) and 27 % (w/w), respectively. The test of colorfastness against washing showed that both water extract of the white frangipani dan hibiscus leaves has a pretty good average quality in staining scale but less in grayscale. The test against rubbing shows that the type of fixer used affects the quality of the colorfastness, but in general, the water extract of white frangipani leaves gives better quality as a potential of natural dye than hibiscus leaves</p>
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Adedej, A. L., O. T. Adedosu, J. A. Badmus, G. E. Adeleke, I. R. Afolayan, and I. F. Olarinde. "Aqueous extract of Hibiscus Sabdariffa calyx modulates gentamicin activity in rats." Asian Pacific Journal of Health Sciences 3, no. 3 (July 2016): 178–87. http://dx.doi.org/10.21276/apjhs.2016.3.3.27.
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Anusha, M. R., Veluswamy Bhuvaneshwari, Manokaran Kalaiselvi, Akbar Ali Amrin, and Ramasamy Amsaveni. "In Vitro Anti-Inflammatory Activity of Hibiscus Schizopetalus (Dyer) Hook. f." Advanced Science, Engineering and Medicine 12, no. 4 (April 1, 2020): 484–88. http://dx.doi.org/10.1166/asem.2020.2545.
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Medicinal plants act as an important source of drug with potential therapeutic effects. The present study focuses on the in vitro anti-inflammatory activity of Hibiscus schizopetalus (Dyer) Hook. f. (leaves and flowers) extracts. The cold water and hot water extract was prepared for plant sample without drying and whereas cold water, hot water, methanol, ethanol extract was prepared for the plant sample after drying. The present study on extract of H. schizopetalus demonstrated in vitro anti-inflammatory properties as evidenced by inhibition of albumin denaturation, membrane stabilization test and proteinase inhibitory action. This justifies that the traditional use of this plant in treatment of pains and inflammation.
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Baihaqi, Ridwan, M. Yulis Hamidy, and Eka Bebasari. "Efek Analgetik Ekstrak Etanol Kelopak Rosella (Hibiscus sabdariffa L) Pada Mencit Jantan (Mus musculus)." Jurnal Ilmu Kedokteran 8, no. 2 (December 29, 2017): 64. http://dx.doi.org/10.26891/jik.v8i2.2014.64-69.
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Indonesia has been known for its traditional medicine and one of the plants used for tradisional medicine is Hibiscussabdariffa L. The roselle (Hibiscus sabdariffa L) calyx has antipyretic effect by blocking prostaglandin synthesis. Thepurpose of this study was to find out the existences of analgesic effect of roselle (Hibiscus sabdariffa L) calyx ethanolextract. This experimental research used a post test only design that was conducted on 25 male mices weighting 20-35 g. The mices were divided into 5 treatment groups and given the CMC-Na 0,5% (negative control), asetosal 65 mg/kgBB (positive control) and groups of roselle (Hibiscus sabdariffa L) calyx ethanol extract dose of 200 mg/kgBB,400 mg/kgBB and 800 mg/kgBB. Pain stimulus was given chemically by using 1% acetic acid that injectedintraperitoneally 30 minutes after sample was given the extract, the response of the mices will be writhing. It calledpositive response if percentage potency of analgesic e” 50%. The result of this research showed that all dosage ofroselle (Hibiscus sabdariffa L) calyx ethanol extract had an analgesic effect.
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Chimurkar, Leena, Deepashri Maraskolhe, and Prachi Kamble. "In vitro evaluation of an antibacterial effect of rose leaves and petals extract along with antibiotics on Escherichia coli." International Journal of Research in Medical Sciences 8, no. 7 (June 26, 2020): 2601. http://dx.doi.org/10.18203/2320-6012.ijrms20202902.
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Background: Today Herbal medicine are commonly used because of their easy availability and affordability. It is more closely corresponds to the patient’s belief, concerns about the adverse effects of chemical (synthetic) medicines, persuade a desire for more personalized health care, and allows significant public access to health information. The major use of herbal medicines is for health promotion and treatment for chronic, as opposed to life-threatening, conditions. Usage of traditional remedies increases when conventional medicine is less effective in the treatment of disease, such as in advanced cancer and in the face of new infectious diseases. The hibiscus Rosa sinensis plant extract have multiple organic component like flavonoids, tannins, alkaloids, triterpenoids which are known to have antibacterial activity against E. coli, P aeruginosa, Salmonella species. Phytotherapy is considered to be less toxic and minimal or no side effects in comparison to modern allopathic medicines. Therefore, in today’s scenario there has been reappearance of interest developed in herbal medicine. Therefore, the study was planned to look for antibacterial activity of extract of petals and leaves of hibiscus Rosa sinensis against.Methods: Escherichia coli isolated from different clinical samples along with resistant antibiotics. All resistant isolates were tested along with hibiscus Rosa sinensis extract both leaves and petals ex-tract with resistant antibiotics.Results: We found that out of total 51, 35 (68.63%) were resistant E. coli isolates to different antibiotics and 85.72% were from urine samples. Enhancement effect in zone size with many resistant antibiotics was seen individually with both extracts. Antibiotics like ampicillin, cefotaxime, cotrimaxazole, cefuroxime, piperacillin, gentamycin showed more enhancement with rose petal extract than leaves. While antibiotic like levofloxacin (5%) showed enhancement only with leaves extract and not with the petals extract.Conclusions: It was found that Rose petal extract showed more antibacterial activity along with the antibiotics as compare to Rose leaves extract.
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De Vilela Silva, Eugênia Telis, Henrique Valentim Moura, Rossana Maria Feitosa de Figueiredo, Alexandre Jose De Melo Queiroz, and Inácia Dos Santos Moreira. "Production and characterization of mixed pineapple and hibiscus jam." REVISTA AGRO@MBIENTE ON-LINE 13 (August 1, 2019): 155. http://dx.doi.org/10.18227/1982-8470ragro.v13i0.5523.
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Pineapple is a food rich in vitamins, antioxidants and bromelain, but because it has high water content it becomes a highly perishable fruit. Hibiscus is a plant with flowers rich in phytochemicals that can be used as an ingredient to add value to processed products. The objective of this work was to prepare mixed jams using pineapple with hibiscus extract at different concentrations and to characterize the physicochemical properties
of the jams produced. Experimental design was completely randomized with four replicates. The treatments consisted of three concentration levels of hibiscus extract (5, 10 and 15%) in pineapple jelly (50:50). Analyzed physical-chemical characteristics were: water content, water activity, ash, lipids, total sugars, reducing and non-reducing sugars, vitamin C, total titratable acidity, pH and total soluble solids. It was found that increasing
concentrations of hibiscus in the formulations significantly influenced resulting jam composition. Increasing hibiscus concentration reduced the total sugars content and increased ascorbic acid content, acidity, total soluble solids and ash, as well as total anthocyanins and flavonoids contents. Among the jams produced, the formulation with 15% hibiscus was distinguished by its high content of ascorbic acid, flavonoids and anthocyanins. The addition of hibiscus to pineapple jelly improves the nutritional and functional value of the resulting jams, so it can be considered a high potential ingredient for this type of product.
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Ukoha, U. U., S. I. Mbagwu, G. U. Ndukwe, and C. Obiagboso. "Histological and Biochemical Evaluation of the Kidney following Chronic Consumption of Hibiscus sabdariffa." Advances in Biology 2015 (November 17, 2015): 1–5. http://dx.doi.org/10.1155/2015/486510.
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Hibiscus sabdariffa L. has been used traditionally as herbal medicine and has been documented to have a broad range of therapeutic effects. The present study aimed to investigate the effects of chronic administration of aqueous extract of flowers of Hibiscus sabdariffa on the histology of the kidney and some biochemical indices of renal function in male Wistar rats. Twenty (20) Wistar rats were randomly divided into four (4) groups of five rats each. The extract was administered orally in doses 200, 500, and 800 mg/kg body weight for 21 days. The kidney was harvested and processed histologically and blood samples were taken for biochemical assays. The histological results showed dose dependent pathological states and the biochemical analysis revealed a dose dependant variation in renal indices. These results suggest that chronic administration of aqueous extract of Hibiscus sabdariffa may be toxic to the kidney.
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Hassan, B. H., and A. I. Hobani. "Flow properties of Roselle (Hibiscus sabdariffa L.) extract." Journal of Food Engineering 35, no. 4 (March 1998): 459–70. http://dx.doi.org/10.1016/s0260-8774(98)00044-2.
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AL-KAHTANI, HASSAN A., and BAKRI H. HASSAN. "Spray Drying of Roselle (Hibiscus sabdariffa L.) Extract." Journal of Food Science 55, no. 4 (July 1990): 1073–76. http://dx.doi.org/10.1111/j.1365-2621.1990.tb01601.x.
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Calliari, Caroline Maria, Roberta Campardelli, Margherita Pettinato, and Patrizia Perego. "Encapsulation of Hibiscus sabdariffa Extract into Zein Nanoparticles." Chemical Engineering & Technology 43, no. 10 (August 31, 2020): 2062–72. http://dx.doi.org/10.1002/ceat.202000194.
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TIKO, Gerard Hodevé, Adandé MEDJIGBODO, Rafiou ADAMOU, Abdou Madjid Olatoundé AMOUSSA, Luc Salako DJOGBENOU, and Latifou LAGNIKA. "Scientific Baseline Information for the Potential Use of Hibiscus surattensis L against Malaria: Phytochemistry and Biological Studies." Journal of Drug Delivery and Therapeutics 10, no. 5-s (October 15, 2020): 127–35. http://dx.doi.org/10.22270/jddt.v10i5-s.4491.
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Background and aim: Hibiscus surattensis L. is a medicinal plant widely used traditionally in Benin to treat malaria. This study was designed to investigate antiplasmodial activity, hemolytic power, acute toxicity, antioxidant activity and phytochemical content of H. surattensis.
Methodology: Bioassay-guided isolation approach was adopted and extracts and fractions collected were continuously tested in vitro against Chloroquine-sensitive and field isolate strains of Plasmodium falciparum by immune-dosage of Plasmodium lactate dehydrogenase. Hemolytic effect and acute toxicity of extracts were evaluated respectively on human erythrocytes and according to OECD guideline N°423. 2, 2-diphenyl-1- picrylhydrazyl) radical scavenging, Ferric reducing antioxidant power, superoxyde radical scavenging and hydrogen peroxide radical scavenging methods were used to investigate antioxidant activity.
Results and conclusion: The best antiplasmodial activity was obtained with ethanolic extract of H. surattensis L. against field isolate (IC50 = 5.03±0.31 µg/mL) and Chloroquine -sensitive (IC50 = 7.55±0.59 µg/mL) whereas aqueous extract exhibited moderate activity. Bioassay-guided fractionation of ethanolic extract shows progressive decrease of the antiplasmodial activity. Both extracts exhibited strong antioxidant activity, hemolytic power less than 1%. No mortality of rats was recorded with ethanolic extract at 2000 mg/kg body weight. Flavonoids, anthraquinones, coumarins, and triterpenes are present in both extracts with tannins in the ethanolic extract. In summary, the extracts of H. surattensis have interesting antiplasmodial and antioxidant properties probably resulting from a synergetic action of their secondary metabolites, without toxicity effect on rats and human erythrocytes. These findings strengthen the traditional use of H. surattensis as antimalarial plants.
Keywords: Hibiscus surattensis; antiplasmodial; antioxidant; toxicity; phytoconstituents.
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Hernawan, Hernawan. "INHIBITION OF HUMAN LOW-DENSITY LIPOPROTEINS OXIDATION BY Hibiscus radiatus CUV. CALYCES EXTRACT." Indonesian Journal of Chemistry 8, no. 1 (June 17, 2010): 111–13. http://dx.doi.org/10.22146/ijc.21655.
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Hibiscus radiatus Cuv calyces extracts rich in polyphenols was screened for their potential to inhibit oxidation of human low-density lipoproteins-cholesterol (LDL-C) in vitro. The inhibition of LDL-C oxidation (antioxidant activity) was determined by measuring the formation of conjugated dienes and thiobarbituric acid reagent substances (TBARS). LDL-C oxidation was carried out in the presence of H. radiatus Cuv calyces extract (20 and 50 μM). CuSO4 (10 μM) was used as the oxidation initiator and butylated hydroxytoluene (BHT) at 50 μM was used as standard antioxidant. The protective effect of H. radiatus Cuv. calyces extract toward human low-density lipoproteins, complex lipid system was demonstrated by significant increase lag time (> 103 min), diminished of the propagation rate (44 %), and diminution of conjugated dienes formation 59.42 % (50 μM) compared to control. Keywords: antioxidant, conjugated dienes, Hibiscus radiatus Cuv, low-density lipoproteins-cholesterol
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Pakaya, David, Marzela Dewi, Arlita Leniseptaria Antari, and Indah Saraswati. "Ekstrak Hibiscus rosa-sinensis L. Memperbaiki Kadar Glukosa Darah Puasa dan Jumlah Sel Mast pada Model Tikus Diabetes Terinfeksi Mycobacterium tuberculosis." Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) 6, no. 1 (March 2, 2020): 14–19. http://dx.doi.org/10.22487/j24428744.2020.v6.i1.13930.
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Background: The condition of hyperglycemia in Diabetes mellitus (DM) causes a disruption of the function of phagocytes that can facilitate M. tuberculosis infection. Immunostimulant from natural ingredients can help improve the condition of hyperglycemia an immune function.
Objective: To analyze the effect of Hibiscus rosa-sinensis L. extract on fasting blood glucose level and the number of mast cell in TB-DM mouse model.
Methods: This experimental study used 27 male Wistar rats, 8 weeks of age, 200-230 g weight, divided into 3 groups, K: Normal control group, P1: TB-positive mouse group P2: Group rat TB-DM + Hibiscus rosa-sinensis L extract dose 250 mg/kgBW and 3 time measurement of H + 7 DM, H + 14 DM and H + 21 DM. TB-DM model mice were prepared with the administration of nicotinamide (NA) 100 mg/kgBW and streptozotocin (STZ) 65 mg/Kg BW, TB agent 1.5 x 105 CFU. Fasting blood glucose levels were measured by GOD-PAP method and Pulmonary tissue is excised, made in paraffin blocks and stained with blue Toluidine to count the mast cells.
Results: There was an improvement in the mean fasting blood glucose levels in the group treated with the Hibiscus rosa-sinensis L. extract of 250 mg/kgBW compared to the TB-DM group without extract therapy followed by decreased the number of mast cell.
Conclusion: Hibiscus rosa-sinensis L. extract. improves fasting blood glucose levels and decreases the number of mast cell of TB-DM rat model.
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Windyaswari, Ari Sri, Yenni Karlina, and Amalia` Junita. "Pengaruh Teknik dan Pelarut Ekstraksi Terhadap Aktivitas Antioksidan dari Empat Jenis Ekstrak Daun Rosella (Hibiscus sabdariffa L.)." Talenta Conference Series: Tropical Medicine (TM) 1, no. 3 (December 19, 2018): 014–19. http://dx.doi.org/10.32734/tm.v1i3.254.
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Tanaman rosella merupakan tanaman asli dari benua Asia (India hingga Malaysia) dan benua Afrika. Kultivasi bagian bunga, daun, dan biji dari tanaman rosella (Hibiscus sabdariffa L.) telah lama digunakan oleh masyarakat sebagai bahan makanan dan pengobatan empiris. Hasil penelitian sebelumnya menunjukkan tanaman rosella memiliki aktivitas farmakologi sebagai antikanker, antibakteri dan antioksidan. Telah dilaporkan bahwa bagian daun dan akar rosella dilaporkan mengandung senyawa fenolik terbanyak dibandingkan dengan bagian lainnya. Penelitian ini bertujuan untuk mengetahui pengaruh teknik dan pelarut ekstraksi terhadap aktivitas antioksidan dari empat jenis ekstrak daun rosella (Hibiscus sabdariffa L.). Teknik ekstraksi yang dilakukan adalah maserasi, infus dan refluk menggunakan pelarut air dan pelarut etanol. Aktivitas peredaman radikal bebas DPPH teridentifikasi pada pola kromatogram lapis tipis dengan penampak bercak DPPH 0,2% dari seluruh ekstrak daun rosella. Pengujian aktivitas antioksidan menggunakan metode peredaman radikal bebas DPPH dilakukan terhadap seluruh ekstrak daun rosella, yaitu ekstrak air teknik maserasi (AM), ekstrak air teknik infus (AI), ekstrak etanol teknik maserasi (EM), serta ekstrak etanol teknik refluk (ER). Nilai IC50 ekstrak AM, AI, EM dan ER berturut-turut adalah: 0,00056 ppm (sangat kuat); 0,00057 (sangat kuat); 0,00044 ppm (sangat kuat); 0,00092 ppm (sangat kuat). Metode penyarian metabolit sekunder optimal untuk aktivitas antioksidan pada daun rosella adalah teknik ekstraksi maserasi dengan pelarut etanol (ER).
Rosella is one of a native plant from Asia (India to Malaysia) and Africa. The Flowers, leaves, and seeds cultivated from rosella (Hibiscus sabdariffa L.) have been used in folk medicine as food and empirical treatment. The previous study reported the pharmacological activities of rosella as anti-cancer, anti-bacterial and anti-oxidant. It has been reported that leaf and root of rosella found to have phenolic compounds as the major components. This research was conducted to evaluate the effect of extraction technique and solvent on the anti-oxidant activity of four extracts of rosella leaves (Hibiscus sabdariffa L.). The extraction techniques include maceration, infusion and reflux with water and ethanol. Scavenging activities of DPPF free radical of all rosella leaves extracts were identified by thin layer chromatography, indicated by DPPH 0,2% reagent. The evaluation of antioxidant activity using scavenging DPPH free radical method was performed to all rosella leaves extract, including water extract by maceration method (AM), water extract by infusion method (AI), ethanol extract by maceration method (EM), ethanol extract by reflux method (ER). The IC50 values of AM, AI, EM and ER were 0,00056 ppm (very strong); 0,00057 (very strong); 0,00044 ppm (very strong); 0,00092 ppm (very strong). The most optimum method to extract secondary metabolite with anti-oxidant properties was maceration with ethanol (ER).
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Krishnamurthy, Zuliana Muhamad Ridzwan, Nurzalina Abdul Karim Khan, Insathe Mohd Ali, Mohamad Dayoob, and Shahad Shakho Hussein. "Hibiscus sabdariffa extract as anti-aging supplement through its antioxidant and anti-obesity activities." Biomedical Research and Therapy 7, no. 1 (January 29, 2020): 3572–78. http://dx.doi.org/10.15419/bmrat.v7i1.584.
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Introduction: Imbalance between total energy intake and expenditure causes accumulation of excess fat and sugar in the body which leads to development of diabetes mellitus type II, obesity, and metabolic syndrome. These harmful diseases accelerate aging and cause fatal metabolic disorders as people age. Inhibition of pancreatic lipase, and alpha glucosidase digestive enzymes is a step that can reduce excess fat and sugar from the body, which is an essential component of healthy aging.
Methodology: In this study, aqueous and 50% ethanolic extracts of Hibiscus sabdariffa were investigated for their inhibitory activities on pancreatic lipase and alpha-glucosidase, in addition to their antioxidant activities (using UV-vis spectrophotometer).
Results: Both extracts displayed antioxidant properties, indicated by IC50 of 5166.80 mg/mL for H. sabdariffa aqueous extract and 2809.10 mg/mL for H. sabdariffa 50% ethanolic extract. The extracts also suppressed the activities of pancreatic lipase and alpha-glucosidase enzymes, which suggests possible antiobesity and anti-diabetic activities. H. sabdariffa aqueous extract inhibited pancreatic lipase activity with IC50 of 167.5+/-12.7 mg/mL, whereas H. sabdariffa 50% ethanolic extract inhibited the enzyme with an IC50 of 790.65+/-16.02 mg/mL. Both H. sabdariffa aqueous and ethanolic extracts also successfully inhibited alpha-glucosidase enzyme activity with IC50 949.88 +/-10.83 mg/mL, and 378.33 +/-4.20 mg/mL, respectively.
Conclusion: Taken together, the outcome of the investigations offers the possibility of the extracts as an anti-obesity, anti-diabetic, anti-metabolic and anti-aging agent, which can be developed into supplements for adults to prevent the occurrence of these prevalent diseases and delay the onset and effects of aging.
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Sun, Jun, and Tongxin Zhang. "Study on the Degradation Effect of Plant-Derived Active Ingredients on Organophosphorus Pesticides." Research on World Agricultural Economy 1, no. 1 (October 21, 2020): 6. http://dx.doi.org/10.36956/rwae.v1i1.162.
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In order to explore new ways and methods for the degradation of organophosphorus pesticides, the degradation effects of plant-derived active ingredients on three organophosphorus pesticides were studied. Mix Rhubarb, Pittosporum bark, Hibiscus bark, and Chinese gall in 9:4:3:2 parts by mass, crush and soak in water, use GC-MS quantitative detection method, rapid pesticide residue measurement method, and compare the organic phosphorus before and after the test Pesticide concentration changes, clarify its degradation effect on organophosphorus pesticides. The results showed that the degradation rates of chlorpyrifos and parathion were 93.2% and 92.9% in the extract within 2 minutes; the degradation rate of dichlorvos in the extract within 17 hours was 66.67%, and the degradation of chlorpyrifos within 11 hours the rate is 48.69%. This study shows that the extracts of rhubarb, sea tongs bark, hibiscus bark, and gallnut have significant degradation effects on chlorpyrifos, parathion, dichlorvos and other organophosphorus pesticides.
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Andraini, Trinovita, and Sophie Yolanda. "Prevention of insulin resistance with Hibiscus sabdariffa Linn. extract in high-fructose fed rat." Medical Journal of Indonesia 23, no. 4 (January 27, 2015): 192–6. http://dx.doi.org/10.13181/mji.v23i4.848.
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Background: Dyslipidemia and stress oxidative play an important role as the cause of insulin resistance. One herb that has potent antioxidant effect and may improve dyslipidemia is Hibiscus sabdariffa Linn. The aim of this study was to evaluate the effect of Hibiscus sabdariffa Linn. extract on fasting blood glucose level, fasting blood insulin level, and insulin resistance index (HOMA-IR) in high-fructose fed rat.Methods: This was an experimental study in 25 Sprague-Dawley rats which were administered with a high-fructose diet (10% ad libitum) and Hibiscus sabdariffa Linn. extract at a dose of 100, 200, and 400 mg/kgBW/d simultaneously for 5 weeks. At the end of study, fasting blood glucose level, fasting blood insulin level and insulin resistance index (HOMA-IR) were measured.Results: Fasting blood glucose, blood insulin, and HOMA-IR level of rats given high-fructose diet with Hibiscus sabdariffa Linn. at dose 100 mg/kgBW/d were not significantly different than the group of rats given only high-fructose fed. While at the dose of 400 mg/kgBW/d, they were significantly lower than the group given only high-fructose fed (4.84 mmol/L vs 6.11 mmol/L, 0.07 µU/L vs 0.3 µU/L, and 0.02 vs 0.08 respectively).Conclusion: Oral administration of Hibiscus sabdariffa Linn. could prevent the development of insulin resistance induced by high-fructose diet in the rat.
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Goswami, Monmi, Debjani Baruah, and Archana Moni Das. "Green synthesis of silver nanoparticles supported on cellulose and their catalytic application in the scavenging of organic dyes." New Journal of Chemistry 42, no. 13 (2018): 10868–78. http://dx.doi.org/10.1039/c8nj00526e.
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Al-fawwaz, Abdullah T., and Khaled A. Al-Khaza'leh. "Antibacterial And Antifungal Effect Of Some Natural Extracts And Their Potential Use As Photosensitizers." European Scientific Journal, ESJ 12, no. 6 (February 29, 2016): 147. http://dx.doi.org/10.19044/esj.2016.v12n6p147.
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One of the threats on the health sector is the appearance of multi antibiotic resistant microorganisms. Thus, this occurs because of using natural and synthetic antimicrobial agents in excessive doses. Now, many scientists all around the world are performing research on natural extracts that have antimicrobial activity using photodynamic therapy (PDT) which involves light activation. The aim of this study, however, is to evaluate the effect of photodynamic antimicrobial chemotherapy using extracts from Pomegranate (Punica granatum), Cinnamon (Cinnamomum verum), Roselle (Hibiscus sabdariffa), Carob (Ceratonia siliqua), and Nigella or black cumin oil (Nigella sativa) against two gram-positive and two gram-negative bacteria (Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa) respectively; and three different fungi (Aspergillus niger, Mucor sp., and Rhizopus sp.), using agar well diffusion method. The prepared methanolic extracts presented absorption between 300 and –900 nm wavelengths. The maximum activity of Roselle (Hibiscus sabdariffa) was against Staphylococcus aureus in total darkness with 52.5 ± 2.5 mm inhibition zone diameter. Also, the most sensitive fungi was Mucor sp. Cinnamon (Cinnamomum verum) extract was with 31.0 ± 1.0 mm inhibition zone diameters. The results showed that there was an inhibitory effect of Cinnamon (Cinnamomum verum) and Roselle (Hibiscus sabdariffa) extracts against most of the tested species.
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Malacrida, Alessio, Valeria Cavalloro, Emanuela Martino, Arianna Cassetti, Gabriella Nicolini, Roberta Rigolio, Guido Cavaletti, et al. "Anti-Multiple Myeloma Potential of Secondary Metabolites from Hibiscus sabdariffa." Molecules 24, no. 13 (July 9, 2019): 2500. http://dx.doi.org/10.3390/molecules24132500.
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Multiple myeloma (MM) belongs to hematological cancers and its incidence is increasing worldwide. Despite recent advances in its therapy, MM still causes many deaths every year. In fact, current therapies sometimes fail and are associated with severe adverse effects, including neurotoxicity. As a part of our ongoing efforts to discover new potential therapies against MM, we prepared Hibiscus sabdariffa extracts obtained by a microwave-assisted solvent extraction and investigate their activity by in vitro assays on the RPMI-8226 cell line. The bioguided fractionation of the crude ethanolic extract allowed the identification of HsFC as the most effective extract. We assessed cell viability (MTT and Tripan blue test), cell migration (Boyden chamber assay), and neurotoxicity (DRG neurotoxicity assay). The promising results prompted us to further fractionate HsFC and we obtained two molecules effective against RPMI-8226 cells without neurotoxic effects at their active concentrations. Moreover, both compounds are able to significantly reduce cell migration.
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Ali, Badreldin H., Intisar Al-Lawati, Sumyia Beegam, Amal Ziada, Suhail Al salam, Abderrahim Nemmar, and Gerald Blunden. "Effect of Hibiscus sabdariffa and its Anthocyanins on Some Reproductive Aspects in Rats." Natural Product Communications 7, no. 1 (January 2012): 1934578X1200700. http://dx.doi.org/10.1177/1934578x1200700115.
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An aqueous extract of Hibiscus sabdariffa L. is a common beverage in many parts of the world. Reports on its effect on reproduction are conflicting, with anecdotal evidence that the plant is an aphrodisiac, while others report that it is estrogenic, and adversely affects spermatogenesis in rats. We have studied the effect of different concentrations of aqueous extracts of H. sabdariffa calyces (10%, 15% and 20%) used as drinking water for 10 consecutive weeks, and its anthocyanins (50, 100, 200 mg/kg for 5 days, orally) on the weight and histology of the testis, and on some biochemical constituents in testicular homogenates, in addition to the plasma concentrations of testosterone, luteinizing hormone and estradiol. The possible presence of an estrogenic effect of the extract and anthocyanins on the uteri of immature female rats was also tested. Neither the H. sabdariffa extract nor the anthocyanins significantly altered either testicular weight and histology, or uterus weight. Plasma concentrations of the three hormones studied, the testicular concentrations of protein, reduced glutathione and total cholesterol, and superoxide dismutase activity were all insignificantly affected by either the extract or the anthocyanins, except for a slight, but statistically significant, decrease in testicular protein concentration caused by the 15% aqueous extract when compared with controls. These results suggest that H. sabdariffa exerts no adverse effect on the male reproductive system. Consumption of H. sabdariffa aqueous extract inhibited the growth of the rats compared with the controls.
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Lusida, Terrence Timothy Evan, Bambang Hermanto, and Sudarno Sudarno. "THE ANTIBACTERIAL EFFECT OF ROSELLE (Hibiscus sabdariffa) EXTRACT AGAINST Staphylococcus epidermidis IN VITRO." Indonesian Journal of Tropical and Infectious Disease 6, no. 4 (August 22, 2017): 88. http://dx.doi.org/10.20473/ijtid.v6i4.3757.
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Infection of Staphylococcus epidermidis is still a common problem in many hospitals. Factor determining biofilm formation makes it harder for atibiotics to cure the infection. Roselle (Hibiscus sabdariffa), a well known traditional medicine plant, is a potential candidate as a drug againts infectious disease. The purpose of this research is to investigate the antibacterial effect of ethanol extract from Roselle (Hibiscus sabdariffa) calyx againts the growth of Staphylococcus epidermidis. Assessment for antibacterial effect is performed using broth diffusion method. The extract is made by maceration of the calyx of Roselle in 96% ethanol. Extracts with concentration of 125, 62.5, 31.25, 15.63, 7.81, 3.90, 1.95, 0.97, 0.48, 0.24 mg/mL are added into separated Mueller-Hinton broths (MHB), which have already been inoculated by Staphylococcus epidermidis. As for bacterial growth control, we used MHB with bacterial inoculation, while sterility control we used mixture of extract and MHB. Then from each broth, the solutions are added into separated nutrition agar plates. Replications are done three times. Clarity and bacterial growth are observed after 24 hours of incubation. However, clarity cannot be observed in 36 broth, but bacterial growth is observed on the plate for concentration 0.97, 0.48, and 0.24 mg/mL. Therefore Minimum inhibitory concentration (MIC) cannot be determined because the extract’s color interfere the observation. While minimum bactericidal concentration (MBC), the last concentration before the concentration where the bacteria are still viable, is 1.95 mg/mL. Based on the result of the research, the Roselle calyx ethanol extract (Hibiscus sabdariffa) through dilution method with a concentration of 1.95 mg / mL can kill Staphylococcus epidermidis and in order to find MIC in collored and turbid solution (before being incubated in incubator), we can consider using agar dilution methode or microdilution methode.