Log in

Relevant bibliographies by topics / High performance liquid Chromatography (HPLC) and Hyphenated techniques / Journal articles

To see the other types of publications on this topic, follow the link: High performance liquid Chromatography (HPLC) and Hyphenated techniques.

Journal articles on the topic 'High performance liquid Chromatography (HPLC) and Hyphenated techniques'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'High performance liquid Chromatography (HPLC) and Hyphenated techniques.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Komal, Zade* Mrunali Ahirrao Madhuri Shirsath Tufail Dana M. B. Nikam. "HPLC and its Hyphenated Techniques: Review." International Journal of Scientific Research and Technology 2, no. 5 (2025): 584–99. https://doi.org/10.5281/zenodo.15529234.

Full text

Abstract:

High Performance Liquid Chromatography (HPLC) is a precise analytical technique used to separate, identify, and quantify components within complex mixtures. Developed in the late 1960s, it operates by pumping a liquid mobile phase under high pressure (50–350 bars) through a column packed with solid adsorbents like silica or polymers, which interact differently with each sample component to achieve separation. HPLC is favored in pharmaceuticals, biotechnology, environmental testing, and food industries for its speed, sensitivity, and high resolution. Hyphenated techniques in liquid chroma

APA, Harvard, Vancouver, ISO, and other styles

2

Patil, Sheetal Vikas, Anup Kishor Nalawade, Rohit Deepak Nalawade, and Shweta Prashant Ghode. "Aliskiren: analytical review for estimation in pharmaceutical formulations." World Journal of Advanced Research and Reviews 6, no. 1 (2020): 019–24. https://doi.org/10.5281/zenodo.4312514.

Full text

Abstract:

Aliskiren (AL) is classified as angiotensin converting enzyme inhibitors (ACEIs) used as antihypertensive drug it acts by dilating blood vessel. These dilation of blood vessel caused due to the renin inhibition. There have been numerous methods developed so far for quantitative estimation of AL in bulk and pharmaceutical dosage form. Pharmaceutical analytical methods include UV Visible Spectrophotometry, Reversed Phase High Performance Liquid Chromatography and hyphenated techniques like Liquid chromatography- Mass Spectrometry. This review paper includes various publications focuses on advanc

APA, Harvard, Vancouver, ISO, and other styles

3

de la Fuente, M. A., and M. Juarez. "Revisión: Aplicación de las técnicas cromatográficas al estudio de triglicéridos y esteroles de la grasa de leche / Review: Application of chromatographic techniques to the study of triglycerides and sterols of milk fat." Food Science and Technology International 5, no. 2 (1999): 103–19. http://dx.doi.org/10.1177/108201329900500201.

Full text

Abstract:

The latest developments in analytical techniques, mainly chromatographic, for the study of triglycer ides (TG) and sterols in milk fat are revised. Gas chromatography (GC) with packed or short capillary columns has been used to separate TG according to their carbon numbers (CN) and can be applied to the detection of foreign fats in milk fat. Combined or hyphenated chromatographic techniques are indispensable in order to identify and quantify individual molecular species of TG: thin layer chro matography (TLC) or high performance liquid chromatography (HPLC) with Ag+ as prefraction stage, follo

APA, Harvard, Vancouver, ISO, and other styles

4

Sagar, Pagade* Sahil Agrawal Prathmesh Shejal Pranjal Chougule Nilesh Chougule. "Detection Of Impurities: A Review On Advance In Impurities Detection And Characterization In Pharmaceuticals By Analytical Techniques." International Journal in Pharmaceutical Sciences 1, no. 12 (2023): 860–79. https://doi.org/10.5281/zenodo.10433875.

Full text

Abstract:

The detection and characterization of impurities in pharmaceuticals are pivotal aspects of ensuring the safety, efficacy, and quality of drug products. This review provides a comprehensive overview of the analytical techniques employed in the identification and quantification of impurities at various stages of pharmaceutical development, manufacturing, and quality control. Chromatographic methods, including High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC), are foundational for their ability to separate complex mixtures. Mass spectrometry (MS) and Nuclear Magnetic Reson

APA, Harvard, Vancouver, ISO, and other styles

5

Anwar, Shumaila, Akbar Khan, Mariyum Jamal, and Muhammad Zain Siddiqui. "Review on the modern analytical advancements in impurities testing." Advances in Analytic Science 6, no. 1 (2025): 3159. https://doi.org/10.54517/aas3159.

Full text

Abstract:

<p>Impurities in active pharmaceutical ingredients (APIs) and pharmaceutical drug products (DPs) lead to broader antithetical effects related to drug safety, efficacy, and regulatory compliance. This review discusses organic, elemental, and inorganic impurities, and residual solvents and stresses their impact on the quality of APIs and pharmaceutical DPs regarding patient safety. It endorses immensely developed contemporary analytical techniques like High-performance Liquid Chromatography (HPLC), and Gas Chromatography (GC), for organic impurities and discusses their hyphenated Mass Spec

APA, Harvard, Vancouver, ISO, and other styles

6

Adhikari, Rudra Prasad, Samima Nasrin Rahman, Suman Lamichhane, and Aditya Bora. "Overview of Analytical Methods for the Determination of H2 Receptor Blockers: A Review." Journal of Drug Delivery and Therapeutics 13, no. 10 (2023): 137–44. http://dx.doi.org/10.22270/jddt.v13i10.6245.

Full text

Abstract:

Overview of various analytical methods for estimating anti-histaminic (H2 Receptor blockers) drugs, particularly for determining their concentration percentage (assay) by analytical methods developed on analytical instruments i.e., UV visible Spectrophotometer, High-Performance Liquid Chromatography, and Hyphenated techniques. The review includes a literature survey of H2 receptor blocker drugs namely cimetidine (Tagamet), ranitidine (Zantac), famotidine (Pepcid, Pepcid AC), and nizatidine (Axid). The examined literature survey addressed chromatographic (HPLC) and UV visible spectrophotometric

APA, Harvard, Vancouver, ISO, and other styles

7

Madhuri, R. Shirsath Sonali A. Waghmare And Pradyumna P. Ige. "A Review On Recent Advances In Development Of RP-HPLC Method." International Journal of Pharmaceutical Sciences 2, no. 8 (2024): 2674–82. https://doi.org/10.5281/zenodo.13242715.

Full text

Abstract:

The development and validation of analytical methods play crucial roles in drug discovery, drug development, and the production of different pharmaceutical formulations. A straightforward, accurate, exact, and targeted RP-HPLC approach was created to determine the drug in pharmaceuticals. Because of its greater selectivity and flexibility for hydrophobic substances, reverse-phase liquid chromatography (RP-HPLC) is the separation method of choice for high-performance liquid chromatography (HPLC). The development of RP HPLC methods has advanced significantly in recent years, with new stationary

APA, Harvard, Vancouver, ISO, and other styles

8

G, Preethi, Siva Prasad M., Prachet P., and Rama Rao N. "Analytical Methods for the Estimation of Levothyroxine and Levothyroxine Sodium in Pharmaceutical Formulations – A Review." International Journal of Pharmaceutical Sciences and Nanotechnology(IJPSN) 16, no. 6 (2023): 7104–13. http://dx.doi.org/10.37285/ijpsn.2023.16.6.10.

Full text

Abstract:

Levothyroxine (L-T4) is used in the treatment of hypothyroidism and is a synthetic levoisomer of the thyroid hormone thyroxine produced by the thyroid gland. Levothyroxine sodium (L-T4S) salt has a high clinical utility. There are numerous studies that have been published that describe analytical methods for the estimation of L-T4 and L-T4S in pharmaceutical formulations and also in various biological matrices like blood, plasma, serum, and urine. This review article focused on analytical methods for estimating L-T4 and L-T4S in pharmaceutical formulations such as Thin Layer Chromatography (TL

APA, Harvard, Vancouver, ISO, and other styles

9

Mandal, Badal Kumar, and Yong-Chien Ling. "Analysis of Chlorophylls/Chlorophyllins in Food Products Using HPLC and HPLC-MS Methods." Molecules 28, no. 10 (2023): 4012. http://dx.doi.org/10.3390/molecules28104012.

Full text

Abstract:

Of the different quality parameters of any food commodity or beverage, color is the most important, attractive and choice-affecting sensory factor to consumers and customers. Nowadays, food industries are interested in making the appearance of their food products attractive and interesting in order to appeal to consumers/customers. Natural green colorants have been accepted universally due to their natural appeal as well as their nontoxic nature to consumers. In addition, several food safety issues mean that natural green colorants are preferable to synthetic food colorants, which are mostly u

APA, Harvard, Vancouver, ISO, and other styles

10

Rutuja, V. Pagare, and Phadtare Dipti. "An Overview of The Hyphenated Separation Technology and its Application in the Pharmacy." Int. J. in Pharm. Sci. 1, no. 1 (2021): 1–11. https://doi.org/10.5281/zenodo.5483833.

Full text

Abstract:

Traditional analytical approaches, including high performance liquid chromatography (HPLC), gas chromatography (GC), and ultraviolet (UV) detection, cannot effectively address the emerging problems of species specificity and sensitivity analysis. A modern analytical technique called transferring is depend upon the traditional use of specific detection of molecules or elements in electrophoresis or chromatography. Currently, the most common methods for the estimation of trace elements include a combination of separation methods and more sensitive detection methods. Previously, this binding meth

APA, Harvard, Vancouver, ISO, and other styles

11

Yu, Xiaoping, Chenglong Liu, Yafei Guo, and Tianlong Deng. "Speciation Analysis of Trace Arsenic, Mercury, Selenium and Antimony in Environmental and Biological Samples Based on Hyphenated Techniques." Molecules 24, no. 5 (2019): 926. http://dx.doi.org/10.3390/molecules24050926.

Full text

Abstract:

In order to obtain a well understanding of the toxicity and ecological effects of trace elements in the environment, it is necessary to determine not only the total amount, but also their existing species. Speciation analysis has become increasingly important in making risk assessments of toxic elements since the toxicity and bioavailability strongly depend on their chemical forms. Effective separation of different species in combination with highly sensitive detectors to quantify these particular species is indispensable to meet this requirement. In this paper, we present the recent progresse

APA, Harvard, Vancouver, ISO, and other styles

12

Jabeen, Nameera, and M. Akiful Haque. "A Review on Bioanalytical Method Development and Validation for Estimation of Drugs by Using Hyphenated Techniques and its Applications on Routine Analysis." Archives of Current Research International 23, no. 2 (2023): 18–35. http://dx.doi.org/10.9734/acri/2023/v23i2556.

Full text

Abstract:

Bioanalytical methods are used to create a protocol that can identify and quantify bioactive molecules and their metabolites in human and animal samples. For determining the medication and its metabolites present in human/animal body, the bioanalytical method is efficient. One of the prominent bioanalytical roles is creating new techniques, validating existing processes, and analyzing samples. Most importantly, a compound can be calculated using many methods and can also be detected using various analytical methods. In specific biological samples, the drugs can be evaluated by different extrac

APA, Harvard, Vancouver, ISO, and other styles

13

Proch, Jędrzej. "UKŁADY ŁĄCZONE OPARTE NA DETEKCJI PRZY UŻYCIU OPTYCZNEJ SPEKTROMETRII EMISYJNEJ W OZNACZANIU FORM METALI I METALOIDÓW." Wiadomości Chemiczne 79, no. 1 (2023): 61–79. https://doi.org/10.53584/wiadchem.2025.1.4.

Full text

Abstract:

Hydride generation (HG) has become a widely used technique for efficient analyte introduction in optical emission spectrometry, although only few hyphenated systems using this technique have been developed. It was decided to fill this gap using a modified spray chamber that allows with conventional nebulizer and/or as hydride generator, i.e. the Multi-mode Sample Introduction System (MSIS). The MSIS unit was used as an interface between high-performance liquid chromatography (HPLC) and inductively coupled plasma optical emission spectrometry (ICP OES) or microwave induced plasma optical emissi

APA, Harvard, Vancouver, ISO, and other styles

14

Komorowicz, Izabela, Adam Sajnóg, and Danuta Barałkiewicz. "Total Arsenic and Arsenic Species Determination in Freshwater Fish by ICP-DRC-MS and HPLC/ICP-DRC-MS Techniques." Molecules 24, no. 3 (2019): 607. http://dx.doi.org/10.3390/molecules24030607.

Full text

Abstract:

Analytical methods for the determination of total arsenic (TAs) and arsenic species (arsenite—As(III), arsenate—As(V), monomethylarsenic acid—MMA, dimethylarsenic acid—DMA and arsenobetaine—AsB) in freshwater fish samples were developed. Inductively coupled plasma mass spectrometry with dynamic reaction cell (ICP-DRC-MS) and high-performance liquid chromatography hyphenated to ICP-DRC-MS were used for TAs and arsenic species determination, respectively. The DRC with oxygen as a reaction gas was used. Sample preparation, digestion, and extraction were optimized. Microwave assisted digestion and

APA, Harvard, Vancouver, ISO, and other styles

15

Shahi, Sanyogita, and Shirish Kumar Singh. "Phytochemical Analysis and Characterization of Cancer-Cure Medicinal Plants: A Comprehensive Review." International Journal of Environmental Sciences 11, no. 10s (2025): 592–606. https://doi.org/10.64252/rg3fjw45.

Full text

Abstract:

This comprehensive review focuses on the current understanding of phytochemical analysis and characterization of medicinal plants recognized for their potential in cancer therapy, particularly emphasising the underlying chemistry. The abstract summarizes the significance of these plants as a rich source of diverse bioactive compounds, highlighting their traditional use and the growing scientific interest in their anticancer properties. The review systematically explores the various chemical classes of phytochemicals implicated in cancer cure, including but not limited to alkaloids, flavonoids,

APA, Harvard, Vancouver, ISO, and other styles

16

Suryavanshi, Vallabh D., Sanjay Sharma, and Jagdish K. Sahu. "Review on Characteristics and Analytical Methods of Remogliflozin Etabonate: An Update." Mini-Reviews in Medicinal Chemistry 22, no. 9 (2022): 1341–50. http://dx.doi.org/10.2174/1389557521666211007115611.

Full text

Abstract:

Abstract: Hyperglycemia and its associated disorders like Diabetes mellitus are engulfing the world’s population at a faster pace. New-age medications like the SGLT 2 inhibitors have found their place in the run to combat DM. Drugs with these properties have proven to be effective in treating hyperglycemia, obesity, and major cardiac disorders. The interesting fact about these drugs is that they act independently of insulin levels in the patient’s body. The fact that they even bypass the side effects shown by currently used anti-diabetic medications has attracted the world’s hope to neutralize

APA, Harvard, Vancouver, ISO, and other styles

17

Wolfender, Jean-Luc, Karine Ndjoko, and Kurt Hostettmann. "LC/NMR in Natural Products Chemistry." Current Organic Chemistry 2, no. 6 (1998): 575–96. http://dx.doi.org/10.2174/1385272802666220130001141.

Full text

Abstract:

Efficient detection and rapid characterisation of natural products play an important role for their study in complex biological matrices such as crude plant extracts. Their preliminary identification at the earliest stage of separation is a strategic element for guiding selective isolation procedures. Hyphenated techniques such as high performance liquid chromatography (HPLC) coupled to UV and mass spectrometry (LC/UV/MS) have proved to be extremely useful in combination with biological screening for a rapid survey of natural products. However, these coupled techniques do not allow a full on-l

APA, Harvard, Vancouver, ISO, and other styles

18

Shuvaeva, O. V., A. A. Zhdanov, T. E. Romanova, P. A. Abramov, and M. N. Sokolov. "Hyphenated techniques in speciation analysis of polyoxometalates: identification of individual [PMo12−xVxO40]−3−x (x = 1–3) in the reaction mixtures by high performance liquid chromatography and atomic emission spectrometry with inductively coupled plasma." Dalton Transactions 46, no. 11 (2017): 3541–46. http://dx.doi.org/10.1039/c6dt04843a.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Kumar, Deepak, Amrendra Kumar, Vinay Kumar, et al. "A Comprehensive Review on Analytical Method Development using RP-HPLC and Recent Advances in Pharmaceutical Applications." Journal for Research in Applied Sciences and Biotechnology 2, no. 2 (2023): 53–60. http://dx.doi.org/10.55544/jrasb.2.2.9.

Full text

Abstract:

The analytical technique of choice for separating, identifying, and quantifying complex mixtures is high-performance liquid chromatography (HPLC). Reverse-phase liquid chromatography (RP-HPLC) is the preferred separation mode for high-performance liquid chromatography (HPLC) due to its adaptability and higher selectivity for hydrophobic compounds. This review article discusses the fundamentals of reversed-phase high-performance liquid chromatography (RP-HPLC). This covers the separation principle, various stationary and mobile phase types, and separation-affecting variables. This article highl

APA, Harvard, Vancouver, ISO, and other styles

20

Chindaphan, Kanokporn, Isaya Thaveesangsakulthai, Suchapa Naranaruemol, et al. "Miniaturized electrocoagulation approach for removal of polymeric pigments and selective analysis of non- and mono-hydroxylated phenolic acids in wine with HPLC-UV." RSC Advances 11, no. 11 (2021): 5885–93. http://dx.doi.org/10.1039/d0ra09089a.

Full text

Abstract:

Electrocoagulation (EC) approach was developed to allow fast sample clean-up step prior to selective analysis of non- and mono-hydroxylated phenolic acids in red wine samples with high performance liquid chromatography hyphenated with UV detection (HPLC-UV).

APA, Harvard, Vancouver, ISO, and other styles

21

Vanitha Madhuri T, Manasa Krishna Pandreka, Geetha Gayatri B, et al. "Advances in High-Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography (UPLC)." Journal of Pharma Insights and Research 2, no. 6 (2024): 039–46. https://doi.org/10.69613/t4nhz921.

Full text

Abstract:

High-Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography (UPLC) are fundamental analytical techniques that have revolutionized chemical analysis across various fields. These chromatographic methods offer exceptional separation capabilities, high resolution, and precise quantification of complex mixtures. HPLC has evolved significantly since its inception, with improvements in column technology, detection systems, and automation. UPLC, introduced as an advancement to conventional HPLC, operates at significantly higher pressures using sub-2-μm particle sizes, res

APA, Harvard, Vancouver, ISO, and other styles

22

Abdul Wadood Ansari, Abhishek Yadav, Ashish Kumar Yadav, Manish Yadav, and Arti. "High-performance liquid chromatography (HPLC): Innovations in analytical techniques and applications." International Journal of Science and Research Archive 15, no. 1 (2025): 155–66. https://doi.org/10.30574/ijsra.2025.15.1.0929.

Full text

Abstract:

High-Performance Liquid Chromatography (HPLC) is a type of column chromatography that is commonly used in biochemistry and analysis to separate, identify, and quantify active chemicals. HPLC is the most often used separation technology for detecting, separating, and quantifying the drug High -Performance Liquid Chromatography (HPLC): A Comprehensive Review of Its, Principle, Detectors, And Application It works on the principle of Affinity chromatography having two phases viz: stationary and mobile phase. The constituent with lower affinity for stationary phase travels faster and vice-versa. HP

APA, Harvard, Vancouver, ISO, and other styles

23

Iqtiar, Md Siddique. "High-Performance Liquid Chromatography: Comprehensive Techniques and Cutting-Edge Innovations." European Journal of Advances in Engineering and Technology 10, no. 9 (2023): 66–70. https://doi.org/10.5281/zenodo.11545742.

Full text

Abstract:

High-Performance Liquid Chromatography (HPLC) has emerged as a pivotal analytical technique, revolutionizing the field of chemical analysis through its precision, versatility, and efficiency. This paper provides a comprehensive overview of HPLC, detailing its fundamental principles, advanced techniques, and wide-ranging applications. We delve into the core components and operational mechanisms of HPLC, elucidating the intricacies of column selection, mobile phase composition, and detection methods. Furthermore, the discussion extends to recent technological innovations that enhance HPLC perfor

APA, Harvard, Vancouver, ISO, and other styles

24

Kumar, Sachil, and Maciej J. Bogusz. "Hyphenated Techniques in Liquid Chromatography and their Applications in Forensic Toxicology." Journal of Forensic Science and Medicine 7, no. 4 (2021): 123–36. http://dx.doi.org/10.4103/jfsm.jfsm_65_21.

Full text

Abstract:

Conventional analytical methods, such as gas chromatography, high performance liquid chromatography (LC), ultra-violet, and others, are ineffective in addressing the increasing number of problems in forensic toxicology. Hyphenated analytical methods, wherein the separation method are coupled or combined with spectral methods, with the help of a proper interface, are the available alternative options. The key benefits of these methods are the requisites of low limits for detection, shorter analytical time, the possibility of automation, better reproducibility, and high precision and repeatabili

APA, Harvard, Vancouver, ISO, and other styles

25

Liu, Zhifu, Zhi Xing, Zhaoyang Li, et al. "The online coupling of high performance liquid chromatography with atomic fluorescence spectrometry based on dielectric barrier discharge induced chemical vapor generation for the speciation of mercury." Journal of Analytical Atomic Spectrometry 32, no. 3 (2017): 678–85. http://dx.doi.org/10.1039/c6ja00421k.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Chetna, Malwal*1 P. B. Jadhav1 Vinod Bairagi 2. "A Comprehensive Review On High-Performance Liquid Chromatography (HPLC)." International Journal in Pharmaceutical Sciences 2, no. 1 (2024): 125–34. https://doi.org/10.5281/zenodo.10472648.

Full text

Abstract:

A collection of techniques called chromatography are employed to extract constituents from a mixture. This approach is divided into two phases; stationery and moveable. The basis for the constituent separation is the variation in the partition coefficients between the two phases. The Greek words "graphein" (to write) and "chroma" (color) are the origin of the word "chromatography". High performance liquid chromatography (HPLC) is an essential technology for determining the quality and quantity of pharmaceutical and environmental samples. It is the most flexible, safe, trustworthy, and efficien

APA, Harvard, Vancouver, ISO, and other styles

27

Chetna, Malwal*1 P. B. Jadhav1 Vinod Bairagi 2. "A Comprehensive Review On High-Performance Liquid Chromatography (HPLC)." International Journal in Pharmaceutical Sciences 2, no. 1 (2024): 125–34. https://doi.org/10.5281/zenodo.10477121.

Full text

Abstract:

A collection of techniques called chromatography are employed to extract constituents from a mixture. This approach is divided into two phases; stationery and moveable. The basis for the constituent separation is the variation in the partition coefficients between the two phases. The Greek words "graphein" (to write) and "chroma" (color) are the origin of the word "chromatography". High performance liquid chromatography (HPLC) is an essential technology for determining the quality and quantity of pharmaceutical and environmental samples. It is the most flexible, safe, trustworthy, and efficien

APA, Harvard, Vancouver, ISO, and other styles

28

Bhati, Chanchal, Neha Minocha, Deepika Purohit, et al. "High Performance Liquid Chromatography: Recent Patents and Advancement." Biomedical and Pharmacology Journal 15, no. 2 (2022): 729–46. http://dx.doi.org/10.13005/bpj/2411.

Full text

Abstract:

Background: Chromatography is defined as a set of techniques that are used for the separation of constituents in a mixture. Introduction: High-Pressure Liquid Chromatography or High-Performance Liquid Chromatography (HPLC) is known as a specialized technique in which columns as well as liquid chromatography are used in the separation, characterizationand investigation of the active moieties existing in the mixture. Objective: Current review focuses on the HPLC technique, including its principles, instrumentation, types, applications, advancements, and patents. Result: HPLC technique is importa

APA, Harvard, Vancouver, ISO, and other styles

29

Kapase, Sachin N. "ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF COMBINATION OF ANTI-ASTHMATIC DRUGS MONTELUKAST AND DOXOFYLLINE." Journal of Medical pharmaceutical and allied sciences 11, no. 6 (2021): 86–91. http://dx.doi.org/10.22270/jmpas.v10i6.1940.

Full text

Abstract:

For qualitative and quantitative analysis, various analytical techniques are available such as Ultraviolet (UV) Spectrophotometry, High-performance liquid chromatography (HPLC), High-performance thin layer chromatography (HPTLC). As per literature survey, there are some UV, HPLC, Ultra-Performance Liquid Chromatography (UPLC) and HPTLC analytical methods are developed for Montelukast and Doxofylline individually and in a combination with other drugs too, since yet there are no significant stability studies indicating HPLC method reported for Montelukast and Doxofylline combinations. In the cur

APA, Harvard, Vancouver, ISO, and other styles

30

Iqtiar, Md Siddique. "Unveiling the Power of High-Performance Liquid Chromatography: Techniques, Applications, and Innovations." European Journal of Advances in Engineering and Technology 8, no. 9 (2021): 79–84. https://doi.org/10.5281/zenodo.11545174.

Full text

Abstract:

High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique in analytical chemistry, offering unparalleled precision and versatility in the separation, identification, and quantification of chemical compounds. This abstract provides a comprehensive overview of the fundamental principles, applications, and advancements in HPLC. HPLC operates on the principles of chromatographic separation, utilizing a liquid mobile phase and a solid stationary phase to separate analytes based on their differential interactions with the stationary phase. The methodology encompasses various mo

APA, Harvard, Vancouver, ISO, and other styles

31

Iqtiar, Md Siddique. "Unveiling the Power of High-Performance Liquid Chromatography: Techniques, Applications, and Innovations." Chemistry Research Journal 9, no. 2 (2024): 62–69. https://doi.org/10.5281/zenodo.11281553.

Full text

Abstract:

<strong>Abstract </strong> High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique in analytical chemistry, offering unparalleled precision and versatility in the separation, identification, and quantification of chemical compounds. This abstract provides a comprehensive overview of the fundamental principles, applications, and advancements in HPLC. HPLC operates on the principles of chromatographic separation, utilizing a liquid mobile phase and a solid stationary phase to separate analytes based on their differential interactions with the stationary phase. The methodo

APA, Harvard, Vancouver, ISO, and other styles

32

S., J. Momin*1 S. R. Shahi2 L. P. Jain3 N. D. Kulkarni4 S. S. Gotpagar5 R. M. Savakhande6. "A Concise Review on Hyphenated Techniques: Liquid Chromatography Coupled Mass Spectroscopy (Lc-Ms/Ms)." International Journal of Pharmaceutical Sciences 3, no. 2 (2025): 1856–68. https://doi.org/10.5281/zenodo.14913274.

Full text

Abstract:

Hyphenated techniques combine spectroscopic and chromatographic methods like LC/MS for toxicology, drug monitoring, pharmacokinetic studies, and bioanalysis with interfaces like APCI and ESI for natural product analysis. Mass spectroscopy (MS) is an analytical technique used to determine the m/e ratio of charged analytes, calculate particle masses, and reveal chemical structures using ionization sources. HPLC and mass spectrometry coupling is complex due to high vacuum requirements, but interfaces like TSP, CFAB, API, and ESI overcome this, integrating detectors with liquid chromatographic sep

APA, Harvard, Vancouver, ISO, and other styles

33

Aditi, Gade* Prasanna Datar. "A Review on Analytical Method Development for Drug Sitagliptin." International Journal of Pharmaceutical Sciences 3, no. 4 (2025): 2713–21. https://doi.org/10.5281/zenodo.15263891.

Full text

Abstract:

The Sitagliptin offer significant therapeutic benefits in managing type 2 diabetes mellitus (T2DM) and associated cardiovascular risks. The development of accurate, precise, and validated analytical methods is crucial for ensuring their efficacy, safety, and regulatory compliance. This review explores various analytical techniques, including High-Performance Liquid Chromatography (HPLC), Reverse Phase HPLC (RP- HPLC), High-Performance Thin Layer Chromatography (HPTLC), Liquid Chromatography-Mass Spectrometry (LC-MS/MS), and UV spectrophotometry, highlighting their advantages, challenges, and v

APA, Harvard, Vancouver, ISO, and other styles

34

Parmar, Raghuveersinh, Dr Priyanka Patil, Dr Chainesh Shah, and Dr Umesh Upadhyay. "A Review Article on Method Development and Validation of Verapamil by RP-HPLC Method." International Journal of Pharmaceutical Research and Applications 09, no. 05 (2024): 977–88. https://doi.org/10.35629/4494-0905977988.

Full text

Abstract:

High performance liquid chromatography, one of the most powerful analytical techniques utilized in the separation, identification, and quantification of complex mixtures. Reverse-phase or RP-HPLC is commonly practiced in high performance liquid chromatography because this technique has the advantages of versatility and appropriateness for more hydrophobic compounds. Fundamentals and Practices of Reversed-Phase HPLC: Part II -- RPHPLC column configurations. It consists of the concept of separation, types of stationary and mobile phases with the variables concerned with the separation. This revi

APA, Harvard, Vancouver, ISO, and other styles

35

Veizerová, L., J. Piešťanský, K. Maráková, et al. "Comparison of column-coupled electrophoresis with liquid chromatography methods in food analysis of quinine." Acta Facultatis Pharmaceuticae Universitatis Comenianae 59, no. 1 (2012): 67–80. http://dx.doi.org/10.2478/v10219-012-0011-y.

Full text

Abstract:

Comparison of column-coupled electrophoresis with liquid chromatography methods in food analysis of quinineComparison of column-coupled electrophoresis with liquid chromatography methods in food analysis of quinine (QUI) is presented in this work. The capillary isotachophoresis (CITP) on-line coupled with capillary zone electrophoresis (CZE) and hyphenated with fibre-based spectrophotometric diode array detection (DAD) was compared with, (i) high performance liquid chromatography (HPLC) method with DAD detection, and (ii) HPLC method with fluorescence detection (FD). These methods were compare

APA, Harvard, Vancouver, ISO, and other styles

36

Wei, Ming-Chi, Yu-Chiao Yang, and Show-Jen Hong. "Determination of Oleanolic and Ursolic Acids inHedyotis diffusaUsing Hyphenated Ultrasound-Assisted Supercritical Carbon Dioxide Extraction and Chromatography." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/450547.

Full text

Abstract:

Oleanolic acid (OA) and ursolic acid (UA) were extracted fromHedyotis diffusausing a hyphenated procedure of ultrasound-assisted and supercritical carbon dioxide (HSC–CO2) extraction at different temperatures, pressures, cosolvent percentages, and SC–CO2flow rates. The results indicated that these parameters significantly affected the extraction yield. The maximal yields of OA (0.917 mg/g of dry plant) and UA (3.540 mg/g of dry plant) were obtained at a dynamic extraction time of 110 min, a static extraction time of 15 min, 28.2 MPa, and 56°C with a 12.5% (v/v) cosolvent (ethanol/water = 82/18

APA, Harvard, Vancouver, ISO, and other styles

37

Edward Raju Gope, Shaik Munnisha Begum, Pujitha Prayer Anisetti, et al. "A Review of Principles, Applications, and Recent Developments in HPTLC and HPLC." Journal of Pharma Insights and Research 2, no. 6 (2024): 056–64. https://doi.org/10.69613/315vge42.

Full text

Abstract:

High-Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC) represent fundamental analytical techniques in modern chemical analysis. These sophisticated separation methods have revolutionized the field of analytical chemistry through their precision, reliability, and versatility. HPTLC, an enhancement of traditional thin-layer chromatography, offers advantages such as minimal sample preparation, simultaneous analysis of multiple samples, and cost-effectiveness. It has found extensive applications in pharmaceutical analysis, natural product research, and

APA, Harvard, Vancouver, ISO, and other styles

38

Ajeel, Zaineb H., and Maha N. Hamad. "Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq." Iraqi Journal of Pharmaceutical Sciences ( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512) 29, no. 2 (2020): 202–13. http://dx.doi.org/10.31351/vol29iss2pp202-213.

Full text

Abstract:

The target of this study was to study the natural phytochemical components of the head (capsule) of Cynara scolymus cultivated in Iraq. The head (capsule) of plant was extracted by maceration in70% ethanol for 72 hours, and fractioned by hexane, chloroform and ethyl acetate. Preliminary qualitative phytochemical screening was performed on the ethyl acetate fraction for capsule was revealed the presence of flavonoid and aromatic acids. These were examined by (high -performance liquid chromatography) (HPLC diodarray), (high- performance thin-layer chromatography)(HPTLC). Flavonoids were isolated

APA, Harvard, Vancouver, ISO, and other styles

39

P., Chandrashekhar Reddy*1 2. G. Sampath Kumar Reddy3 and B. Jainendra Kumar 4. "Advancements in HPLC Techniques for Detecting and identification of Process-Related and Degradants Impurities in Pharmaceutical Compounds." International Journal of Pharmaceutical Sciences 3, no. 2 (2025): 527–46. https://doi.org/10.5281/zenodo.14834264.

Full text

Abstract:

Advancements in High-Performance Liquid Chromatography (HPLC) have significantly improved the detection, identification and quantification of process-related impurities and degradants in pharmaceuticals. Key innovations include Ultra-High-Performance Liquid Chromatography (UHPLC), which provides enhanced resolution and sensitivity, and multi-dimensional chromatography for better separation of complex mixtures. The integration of HPLC with Mass Spectrometry, particularly LC-MS/MS and High-Resolution Mass Spectrometry (HRMS), enables precise identification and quantification of impurities at tra

APA, Harvard, Vancouver, ISO, and other styles

40

Barreca, Salvatore, Maddalena Busetto, Luisa Colzani, et al. "Hyphenated High Performance Liquid Chromatography–Tandem Mass Spectrometry Techniques for the Determination of Perfluorinated Alkylated Substances in Lombardia Region in Italy, Profile Levels and Assessment: One Year of Monitoring Activities During 2018." Separations 7, no. 1 (2020): 17. http://dx.doi.org/10.3390/separations7010017.

Full text

Abstract:

In this research paper, we report a hyphenated technique based on ultra-high performance liquid chromatography–tandem mass spectrometry for the determination of twelve Perfluorinated Alkylated Substances in surface and groundwater samples from Lombardia Region during the monitoring activities in 2018 as new emerging and toxic pollutants. A green analytic method, developed by using an online Solid Phase Extraction coupled with UHPLC-MS/MS and previously validated, was applied for 4992 determinations conducted on 416 samples from 109 different sampling stations. Among the results, PFOS, PFOA, PF

APA, Harvard, Vancouver, ISO, and other styles

41

Qian, Zheng-Ming, Bo-Wen Fang, Hai-Ming Chen, et al. "Online Liquid Microextraction Coupled with HPLC-ABTS for Rapid Screening of Natural Antioxidants: Case Study of Three Different Teas." Journal of Chromatographic Science 58, no. 9 (2020): 875–79. http://dx.doi.org/10.1093/chromsci/bmaa047.

Full text

Abstract:

Abstract In the present study, an online liquid extraction coupled with high-performance liquid chromatography-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (HPLC-ABTS) system for rapid screening of antioxidants in tea samples was proposed. As an example, the tea samples were firstly extracted by online HPLC extractor with mobile phase at 70°C, then the hyphenated HPLC-ABTS was used for the chromatographic separation on a Poroshell EC C18 column by 0.3% aqueous formic acid and acetonitrile with a gradient elution at 1.5 mL·min−1, and the UV and antioxidant chromatograms with detection

APA, Harvard, Vancouver, ISO, and other styles

42

Tuzimski, Tomasz, and Anna Petruczynik. "Review of Chromatographic Methods Coupled with Modern Detection Techniques Applied in the Therapeutic Drugs Monitoring (TDM)." Molecules 25, no. 17 (2020): 4026. http://dx.doi.org/10.3390/molecules25174026.

Full text

Abstract:

Therapeutic drug monitoring (TDM) is a tool used to integrate pharmacokinetic and pharmacodynamics knowledge to optimize and personalize various drug therapies. The optimization of drug dosing may improve treatment outcomes, reduce toxicity, and reduce the risk of developing drug resistance. To adequately implement TDM, accurate and precise analytical procedures are required. In clinical practice, blood is the most commonly used matrix for TDM; however, less invasive samples, such as dried blood spots or non-invasive saliva samples, are increasingly being used. The choice of sample preparation

APA, Harvard, Vancouver, ISO, and other styles

43

Hamza, Ouakouak, Ben Mohamed Moktar, Ben Chohra Mostafa, and Abdelhamid Zeghdaoui. "Phenobarbital Analysis in Biological Matrix (Blood) by High Performance Liquid Chromatography (HPLC)." International Letters of Chemistry, Physics and Astronomy 20 (October 2013): 31–40. http://dx.doi.org/10.18052/www.scipress.com/ilcpa.20.31.

Full text

Abstract:

In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogenous blood components, and optimizing the conditions of chromatographic separation as the composition of the mobile phase consisting of 20% acetonitrile + 20% methanol + 60% ammonium acetate buffer. The extraction with diethyl ether to pH = 4; The chromatographic column, microbondapack ZORBAX C18. A s

APA, Harvard, Vancouver, ISO, and other styles

44

Hamza, Ouakouak, Ben Mohamed Moktar, Ben Chohra Mostafa, and Abdelhamid Zeghdaoui. "Phenobarbital Analysis in Biological Matrix (Blood) by High Performance Liquid Chromatography (HPLC)." International Letters of Chemistry, Physics and Astronomy 20 (October 16, 2013): 31–40. http://dx.doi.org/10.56431/p-6t05wn.

Full text

Abstract:

In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogenous blood components, and optimizing the conditions of chromatographic separation as the composition of the mobile phase consisting of 20% acetonitrile + 20% methanol + 60% ammonium acetate buffer. The extraction with diethyl ether to pH = 4; The chromatographic column, microbondapack ZORBAX C18. A s

APA, Harvard, Vancouver, ISO, and other styles

45

Azhakesan, Ajitha, and Sujatha Kuppusamy. "Canagliflozin: A review with specific focus on analytical methods in biological matrices and pharmaceuticals." Reviews in Analytical Chemistry 41, no. 1 (2022): 287–300. http://dx.doi.org/10.1515/revac-2022-0049.

Full text

Abstract:

Abstract Sodium-glucose transporter 2 inhibitor emerges as the latest group of oral hypoglycemic agents, which shows insulin-independent pathology and provides an upper hand to enhance renal glucose elimination. Canagliflozin (CGN) was the number one drug, approved by FDA on 29th March 2013 for the treatment of type 2 diabetes mellitus. By totting up to its glucose-lowering effects, it exhibits beneficial effects on the heart and potentially on the kidneys. The study aims to summarize various analytical techniques, such as chromatography, spectrophotometry, and hyphenated techniques, such as L

APA, Harvard, Vancouver, ISO, and other styles

46

Ali, Rawdha Mahmood, Aum-El-Bashar H. J. AL-Mossawi, and Lena Samer Mohammed. "Extraction and identification of Carnosines from Alkhshni Fish Liza abu L. by using different techniques." Iraqi Journal of Aquaculture 14, no. 2 (2021): 95–106. http://dx.doi.org/10.58629/ijaq.v14i2.96.

Full text

Abstract:

Carnosines were extracted from alkhshni fish Liza abu L. by using alcohol and water separation,Carnosines were identificated in several ways including spectroscopy identification using UV-visible and Fourier Transform infrared spectrophotometer technology and High Performance Liquid Chromatography (HPLC), spectral diagnostics using Ultraviolet-visible (UV) carnosines single peak showed at 215 nm wavelength and at diagnosis High Performance Liquid Chromatography (HPLC) retention time stood for standard carnosines 2.532 and author carnosines separated from 2.614 minutes alcoholic carnosines and

APA, Harvard, Vancouver, ISO, and other styles

47

Haraguchi, Hiroki, Atsushi Ishii, Takuya Hasegawa, Hirotaka Matsuura, and Tomonari Umemura. "Metallomics study on all-elements analysis of salmon egg cells and fractionation analysis of metals in cell cytoplasm." Pure and Applied Chemistry 80, no. 12 (2008): 2595–608. http://dx.doi.org/10.1351/pac200880122595.

Full text

Abstract:

Salmon egg cells were the subject of investigation for all-elements analysis in a single biological cell, where the elements in egg cells were simultaneously determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) and inductively coupled plasma mass spectrometry (ICP-MS) after microwave-assisted acid digestion. As a result, 74 elements among 78 stable isotope elements were determined or detected, although H, C, N, and O were determined separately by conventional CHN elemental analysis. In addition, the survey of protein-binding metallic elements as well as metalloid ele

APA, Harvard, Vancouver, ISO, and other styles

48

Mahdavijalal, Mohammadreza, Carmine Petio, Giovanni Staffilano, Roberto Mandrioli, and Michele Protti. "Innovative Solid-Phase Extraction Strategies for Improving the Advanced Chromatographic Determination of Drugs in Challenging Biological Samples." Molecules 29, no. 10 (2024): 2278. http://dx.doi.org/10.3390/molecules29102278.

Full text

Abstract:

In the past few decades, considerable scientific strides have been made in the subject of drug analysis in human biological samples. However, the risk caused by incorrect drug plasma levels in patients still remains an important concern. This review paper attempts to investigate the advances made over the last ten years in common sample preparation techniques (SPT) for biological samples based on solid sorbents, including solid-phase extraction (SPE) and solid-phase micro-extraction (SPME), and in particular in the field of molecularly imprinted polymers (MIPs), including non-stimuli-responsiv

APA, Harvard, Vancouver, ISO, and other styles

49

Fukutsu, Naoto, Takao Kawasaki, Koichi Saito, and Hiroyuki Nakazawa. "Application of high-performance liquid chromatography hyphenated techniques for identification of degradation products of cefpodoxime proxetil." Journal of Chromatography A 1129, no. 2 (2006): 153–59. http://dx.doi.org/10.1016/j.chroma.2006.06.102.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Lin, Chao-Zhan, Run-Jing Zhang, Yu-Feng Yao, et al. "Qualitative and Quantitative Analysis of the Major Constituents in WLJ Herbal Tea Using Multiple Chromatographic Techniques." Molecules 23, no. 10 (2018): 2623. http://dx.doi.org/10.3390/molecules23102623.

Full text

Abstract:

Quality control of Chinese herbal tea remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling and famous brand of beverage in China, Wanglaoji Herbal Tea (WLJHT), via a full component quantitative analysis. In this paper, a total of thirty-two representative constituents were identified or tentatively characterized using ultra-high performance liquid chromatography coupled with quadrupole tandem time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Moreover, the quantitative analyses of four

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!