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Dissertations / Theses on the topic 'High performance liquid chromatography'

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Published: 4 June 2021

Last updated: 25 July 2025

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1

Abid, F. M. "High performance liquid chromatography : theory and applications." Thesis, Swansea University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.635837.

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A detailed study was undertaken of both the individual and combined effects of pH, temperature and flow rate on the retention volumes of underivatised amino acids. The relative merits of 3-dimensional S-window diagrams to locate the optimum conditions for the separation of amino acids on a stationary phase comprising 5μ ODS has been successfully performed. The enthalpies of retention of transfer from stationary phase to mobile phase for the amino acids have been measured from the chromatographic data. An isocratic study of the separation of phenylthiohyddation (PTH) amino acids using a 3μ MOS

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2

Li, Zhiguo. "High-performance liquid chromatography analysis of fatty acids and mathematical modeling of liquid chromatography." Ohio : Ohio University, 2001. http://www.ohiolink.edu/etd/view.cgi?ohiou1179157379.

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3

Norcio, Lawrence P. "Stability studies of coal liquid products using high performance liquid chromatography." Morgantown, W. Va. : [West Virginia University Libraries], 1999. http://etd.wvu.edu/templates/showETD.cfm?recnum=984.

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Thesis (Ph. D.)--West Virginia University, 1999.<br>Title from document title page. Document formatted into pages; contains xi, 152 p. : ill. (some col.) Vita. Includes abstract. Includes bibliographical references (p. 127-133).

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4

DONALD, GREGORY THOMAS. "Model Chiral Ionic Liquids for High Performance Liquid Chromatography Stationary Phases." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1214325450.

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5

Edwardson, P. A. D. "High Performance Liquid Chromatography of polynucleotides and proteins." Thesis, London School of Hygiene and Tropical Medicine (University of London), 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376534.

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6

Aggarwal, Pankaj. "High-Performance Polymer Monoliths for Capillary Liquid Chromatography." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/4236.

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This dissertation focuses on improving the chromatographic efficiency of polymeric organic monoliths by characterizing and optimizing the bed morphology. In-situ characterization techniques such as capillary flow porometry (CFP), 3-dimensional scanning electron microscopy (3D SEM) and conductivity measurements were developed and implemented to quantitatively characterize the morphology of poly(ethylene glycol) diacrylate (PEGDA) monoliths. The CFP measurements for monoliths prepared by the same procedure in capillaries with different diameters (i.e., 75, 150, and 250 μm) clearly showed a chang

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7

Benson, Andrew James. "High-performance liquid chromatography (HPLC) and high-performance liquid chromatography mass spectrometry (HPLC/MS) for the analysis of date rape drugs." FIU Digital Commons, 2002. http://digitalcommons.fiu.edu/etd/1602.

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The drugs studied in this work have been reportedly used to commit drug-facilitated sexual assault (DFSA), commonly known as "date rape". Detection of the drugs was performed using high-performance liquid chromatography with ultraviolet detection (HPLC/UV) and identified with high performance-liquid chromatography mass spectrometry (HPLC/MS) using selected ion monitoring (SIM). The objective of this study was to develop a single HPLC method for the simultaneous detection, identification and quantitation of these drugs. The following drugs were simultaneously analyzed: Gamma-hydroxybutyrate (GH

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8

Forssén, Patrik. "Adsorption isotherm parameter estimation in nonlinear liquid chromatography /." Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5971.

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9

Meeson, Stephen Russell. "The separation of enantiomers by high performance liquid chromatography." Thesis, University of Newcastle Upon Tyne, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278414.

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10

Sabharwal, Arvind. "Preparative reversed-phase high performance liquid chromatography of proteins." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361678.

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11

Young, Erepamowei. "Nebulized flame ionization detection in high performance liquid chromatography." Thesis, Loughborough University, 2008. https://dspace.lboro.ac.uk/2134/15141.

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A nebulized flame ionization detector interfaced with LC was examined and found to be more versatile in applications than common LC detectors, such as UV, RID, ELSD and CAD. The technique can be used for both volatile and non-volatile analytes. It is compatible with gradient elution and can be used for the analysis of non chromophore-possessing analytes. The calibration plots of non-volatile analytes were linear in contrast to other aerosol-based detectors, such as ELSD and CAD. The technique was examined in three consecutive stages; optimization of the FID, testing the response patterns of an

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12

Mohd, Noor Laili Rusman. "Determination of transition metals by high performance liquid chromatography." Thesis, Sheffield Hallam University, 1990. http://shura.shu.ac.uk/20176/.

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A simple chromatographic analysis system was developed based on dynamic ion pairing using long chain anionic modifiers with a silica reversed phase based column. The aim was to carry out simultaneous multielement quantitative as well as qualitative analysis of samples containing mixtures of transition metal ions. Among the problems encountered and solved were: metalcontamination of the eluent,finding a suitable pump for the system, minimising the noise and reducing the elution times. Detection of the separated metal ions was enabled by post-column derivatisation prior to introduction to thespe

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13

Sun, Yaqin. "Exploration in Analytical Separations Using High Performance Liquid Chromatography." University of Cincinnati / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148586934.

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14

Nam, Kiebang. "Rapid Isolation and Purification of Plasmid DNA Using High Performance Liquid Chromatography." Thesis, North Texas State University, 1987. https://digital.library.unt.edu/ark:/67531/metadc504136/.

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High Performance Liquid Chromatography (HPLC) has been employed as an analytical tool for the purification and separation of nucleic acids. A Nucleogen DEAE 4000-10 weak anion exchange column, prepacked with modified silica gels, was used to purify and separate a number of Escherichia coli plasmids. Plasmid DNAs were extracted by the alkaline lysis method. The cleared lysate was injected directly onto the Nucleogen column, and the peaks were collected, desalted and analysed by gel electrophoresis. On the chromatogram, the pBR322 formed a distinctive peak at 27 minutes and partial separation wa

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15

Scherzinger, Sabine Hilda. "Phenylpropanolamine : analytical and pharmacokinetic studies using high-performance liquid chromatography." Thesis, Rhodes University, 1988. http://hdl.handle.net/10962/d1004528.

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Phenylpropanolamine (PPA), a synthetic sympathomimetic amine structurally related to ephedrine has been widely used over t he past 40 years as a nasal decongestant and appetite suppressant. It has been the focus of much controversy concerning the efficacy of the drug in its use as an anorectic agent, and due to the side effects caused by the higher doses of PPA required for appetite suppression. Although extensively used, there is little information concerning the determination of PPA in biological fluids and on the pharmacokinetics of this drug. An adaptation of a published high-performance l

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16

Stubbs, Christopher. "High performance liquid chromatographic analysis of erythromycin in serum and urine." Thesis, Rhodes University, 1985. http://hdl.handle.net/10962/d1004581.

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Erythromycin, a macrolide antibiotic used mainly against gram-positive bacteria has been in clinical use since 1952 (1). Previous pharmacokinetic data published on this antibiotic have been derived predominantly from microbiological assay techniques. However, these techniques are relatively imprecise as well as being non-specific and extremely tedious to perform. A novel high performance liquid chromatographic analysis of erythromycin in human serum and urine using U.V. detection at 200 nm and/or electrochemical detection using both an amperometric and a coulometric electrochemical detector is

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17

Roush, John Albert. "Development and characterization of novel detectors for use in flow injection analysis or liquid chromatography." Diss., This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-06062008-170645/.

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18

Zhong, Qiqing. "Chemical separations by distillation and chiral high performance liquid chromatography." [Ames, Iowa : Iowa State University], 2006.

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19

Lei, Tian. "Selenium speciation by high performance liquid chromatography -atomic absorption spectrometry." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55507.

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Selenium has been shown to have multiple biochemical effects ranging from nutrient deficiency at low levels to toxicity at high levels. This duality of concern has led to a demand for increased numbers of highly accurate and precise determinations of selenium in biological materials. A convenient procedure was developed for determining selenoamino acids by HPLC-THG-AAS, based on the derivatization of these analytes with Sanger's reagent. Selenomethionine, selenocystine and selenocysteine (after blocking the free selenol group with phenylmercurio cation) were converted to their N-2,4-dinitrophe

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20

Plant, Stephen Paul. "Novel bonded polymeric stationary phases in high performance liquid chromatography." Thesis, Cardiff University, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278702.

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21

Hamzah, Mohd Suhaimi. "Separation and determination of lanthanides by high performance liquid chromatography." Thesis, University of Salford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308131.

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22

Ritchie, Harald John. "Development of new packing materials for high performance liquid chromatography." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/14283.

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23

Sidwell, Rachel. "Analysis of Glyoxalase II Activity by High Performance Liquid Chromatography." Miami University Honors Theses / OhioLINK, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111089246.

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24

Fong, Yuen Ting. "Quantitative structure retention relationships on using high-performance liquid chromatography." HKBU Institutional Repository, 2003. http://repository.hkbu.edu.hk/etd_ra/426.

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25

Sexton, Danessa Leann. "Determination of lactose by reversed-phase high performance liquid chromatography." [Johnson City, Tenn. : East Tennessee State University], 2004. http://etd-submit.etsu.edu/etd/theses/available/etd-0328104-212023/unrestricted/SextonD041504f.pdf.

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Thesis (M.S.)--East Tennessee State University, 2004.<br>Title from electronic submission form. ETSU ETD database URN: etd-0328104-212023. Includes bibliographical references. Also available via Internet at the UMI web site.

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26

Bawjee, Janita. "An evaluation of UPLC technology for the simultaneous analysis of actives in a multi-active drug." Thesis, Nelson Mandela Metropolitan University, 2011. http://hdl.handle.net/10948/d1008407.

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The evaluation of the potential to use Ultra Performance Liquid Chromatography (UPLC) for the simultaneous quantification of all the actives in a multi-active tablet is described in this work. Part of the evaluation was to ensure that the necessary regulatory requirements were adhered to by ascertaining that an analytical method is suitable for a specific purpose through analytical method validation for the specific multi-active tablet. The UPLC method was also tested for the analysis of similar products, namely tablet formulations that contain similar active ingredients in the same proportion

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27

Junge, Cornelia. "Molekulargenetische Diagnostik des ATRX-Syndroms mittels Denaturing High-Performance Liquid Chromatography (DHPLC)." Doctoral thesis, Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-147264.

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Die DNA-Sequenzierung nimmt in der molekulargenetischen Diagnostik seit vielen Jahren einen großen Stellenwert ein. Zeit- und kosteneffektivere Methoden wie die DHPLC wurden seither für verschiedene Gene etabliert. Ziel dieser Arbeit war die Etablierung der DHPLC für das ATRX-Gen bei Patienten mit Verdacht auf das ATRX-Syndrom. Nach erfolgreicher Etablierung der DHPLC sollten im Rahmen dieser Arbeit 38 Patientenproben des Instituts für Humangenetik der Universität Leipzig mit Verdacht auf das ATRX-Syndrom mittels DHPLC und Sequenzierung untersucht werden. Die Etablierung der DHPLC gelang in d

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28

Bhattacharjee, Rathindra Chandra. "Development of a sensitive and stereoselective high performance liquid chromatographic assay method for propafenone enantiomers in human plasma." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27800.

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Propafenone is a new class 1C antiarrhythmic agent with additional calcium antagonistic and beta-blocking activities. Clinically it is effective in the treatment of supraventricular and ventricular tachycardia, atrial and ventricular fibrillation, ventricular premature contractions and for the management of Wolf-Parkinson-White syndrome. In North America it is still an investigational drug. Propafenone is a chiral drug and is used clinically in the racemic form. The enantiomers of numerous chiral drugs have been shown to differ in their disposition kinetics in the body due to their stereosele

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29

Cooke, Andrew Ralph. "The high quality monitoring of PAHs in potable waters." Thesis, University of Bristol, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336869.

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30

El-Rjoob, Abdul-Wahab. "Dynamic stationary phase modification in reversed-phase high performance liquid chromatography /." free to MU campus, to others for purchase, 1996. http://wwwlib.umi.com/cr/mo/fullcit?p9720536.

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31

Murphy, Kellyann M. "Analysis of Biodiesel Quality Using Reversed Phase High-Performance Liquid Chromatography." Scholarship @ Claremont, 2012. http://scholarship.claremont.edu/pomona_theses/45.

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The alternative fuel biodiesel is produced from the transesterification of vegetable oils or animal fat to fatty acid methyl esters. Pomona has a reactor on campus that can be used to run this reaction and produce biodiesel. The use of biodiesel has been found to lower air pollutant and greenhouse gas emissions, but can be potentially harmful to the engines if it contains impurities. This paper proposes a method using high-performance liquid chromatography to test the quality of biodiesel. This method utilizes instrumentation and materials that are available in Pomona College's Chemistry Depar

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32

Garba, A. "Reverse-phase high performance liquid chromatography of some Group (III) oxinates." Thesis, University of Salford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356196.

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33

Lee, Suk Hean. "Investigation of alcoholic and malolactic fermentation using high performance liquid chromatography." Thesis, Anglia Ruskin University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327472.

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34

Mew, Christopher Derwent. "A prototype multiplexed UV absorbance detector for high performance liquid chromatography." Thesis, King's College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324979.

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35

Narine, Raymond. "A novel microcomputer-controlled transport detector for high-performance liquid chromatography." Thesis, King's College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281718.

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36

Li, F. "Studies of haem biosynthesis and metabolism by high-performance liquid chromatography." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378943.

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37

Kaur, Bulvinder. "Porous graphitic carbon : a new material for high performance liquid chromatography." Thesis, University of Edinburgh, 1986. http://hdl.handle.net/1842/12218.

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This thesis is divided into four parts. In the first part, the history of chromatography is described. Different modes of chromatography are briefly discussed and a survey of stationary phases being used in High Performance Liquid Chromatography (HPLC) is made. The need for a non-polar reversed-phase stationary phase is highlighted. A brief survey of the use of carbon by other workers in liquid chromatography is also made. The second part of the thesis deals with the production and structural study of porous graphitic carbon (PGC). the different stages of production of PGC are discussed. Pore

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38

Wu, Zhiwei. "The study of cell surface phenotypes by high performance liquid chromatography." Thesis, University of Edinburgh, 1990. http://hdl.handle.net/1842/11961.

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Size-exclusion high performance liquid chromatography was developed to study surface membrane proteins of malignant lymphocytes. The membrane proteins were separated in accordance to their molecular sizes or complexed sizes and displayed, in conjunction with SDS-PAGE, as two-dimensional matrices on which the location of proteins is determined by both retardation in the HPLC column and the mobility on the SDS-PAGE gels. Any association or induced-interaction between proteins are reflected by predictable changes of the retention times, thus by the location on the two-dimensional matrix. Individu

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39

Lee, Stephen Thomas. "Evaluation of Enhanced-Fluidity Mobile Phases For High Performance Liquid Chromatography /." The Ohio State University, 1996. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487868114111489.

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40

Liu, Fuyou. "Determination of Hydroquinone in Cosmetic Creams by High Performance Liquid Chromatography." Digital Commons @ East Tennessee State University, 2007. https://dc.etsu.edu/etd/2114.

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Hydroquinone is a most commonly used whitening agent in cosmetics. A high performance liquid chromatography method was developed and validated for the quantitative determination of hydroquinone in creams. Validation parameters such as linearity, precision, accuracy, and limit of detection and limit of quantitation were determined. HPLC was carried by reverses phase technique on a RP-C18 column with a mobile phase of water and methanol (pH 5.0) 70:30. The linearity in the range of 2.0-40.0 μg/mL presents a correlation coefficient of 0.9998. The LOD and LOQ were 0.16 and 0.53 μg/mL, respectively

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41

Bhide, S. R. "Analytical approach to problems in organic chemistry high-performance liquid chromatography." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1989. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3321.

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42

Xiang, Yanqiao. "Capillary Liquid Chromatography Using Micro Size Particles." Diss., CLICK HERE for online access, 2004. http://contentdm.lib.byu.edu/ETD/image/etd531.pdf.

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43

Tang, Jianhua. "Development of a novel gradient chromatofocusing tandem mass spectometry technique for the determination of cationic compounds in biofluids identification of caspace 3 cleavage sites of nhe-1 by high performance liquid chromatography- mass spectrometry /." Cleveland, Ohio : Cleveland State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=csu1247344073.

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Thesis (Ph. D.)--Cleveland State University, 2009.<br>Abstract. Title from PDF t.p. (viewed July 29, 2009). Includes bibliographical references (p. 105). Available online via the OhioLINK ETD Center and also available in print.

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44

Stubbs, Christopher. "Application of high-performance liquid chromatography to the analysis, stability and pharmacokinetics of erythromycin." Thesis, Rhodes University, 1988. http://hdl.handle.net/10962/d1004372.

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Erythromycin is a macrolide antibiotic used mainly in the treatment of infections caused by gram-positive organisms. Erythromycin base is rap idly degraded in acidic media necessitating the use of structurally modified erythromycin derivatives or acid resistant dosage forms in order to decrease gastric inactivation of the drug. The majority of pharmacokinetic studies to-date have utilized relatively non-specific microbiological assay procedures which are unable to differentiate between concentrations of active erythromycin base and the inactive pro-drug derivatives. A high-performance liquid c

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45

Barnes, Karen Wink. "The synthesis and characterization of reversed phase stationary phases for high performance liquid chromatography." Gainesville, FL, 1986. http://www.archive.org/details/synthesischaract00barn.

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46

Vorarat, Suwanna. "A study of some aspects of capillary electrophoresis in drug analysis." Thesis, Robert Gordon University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302582.

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47

Lowe, Christian T. "Retention characteristics of water-soluable calixarene modified mobile phases in HPLC /." Connect to the full text of the document, 1998. http://www.ohiolink.edu/etd/view.cgi?ysu997550028.

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48

Fung, Hau Yee. "Development of the Hong Kong Chinese materia medica Standards monograph of Lini semen." HKBU Institutional Repository, 2018. https://repository.hkbu.edu.hk/etd_oa/531.

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The development of the monograph of a commonly used Chinese medicine, Lini Semen, for the Hong Kong Chinese Materia Medica Standards (HKCMMS) was recorded. HKCMMS is a set of reliable and internationally recognized standards of Chinese medicine. The monograph of Lini Semen was proposed, endorsed, and under editorial phase for the publication of the 9th volume of HKCMMS. In the proposed HKCMMS monograph of Lini Semen, besides regular content such as macroscopic and microscopic identification, and TLC identification, a HPLC fingerprint method and two assay methods were developed. All methods wer

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49

Dotlich, Erin Michele. "ALKYLAMMONIUM FORMATE IONIC LIQUIDS AS SOLVENTS FOR FLUORESCENCE AND LIQUID CHROMATOGRAPHY METHODS." Miami University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=miami1208983119.

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50

Van, Meter David S. III. "Synthesis and Characterization of Surface-Confined Ionic Liquid Stationary Phases for High Performance Liquid Chromatography." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1227275073.

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