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Journal articles on the topic 'High performance thin layer chromatography (HPTLC)'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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1

Saibaba, S. V., and P. Shanmuga Pandiyan. "High Performance Thin Layer Chromatography: A Mini Review." Volume 2, Issue 4: October 2016 – December 2016 2, no. 4 (2016): 219–26. http://dx.doi.org/10.32463/rphs.2016.v02i04.43.

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High Performance Thin layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits, and is often an excellent alternative to GC and HPLC. Applications of HPTLC include phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. HPTLC is useful in detecting chemicals of forensic concern. Various advance techniques in reference to HPTLC like hyphenations in

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Barnali, Das. "High performance thin layer chromatography (HPTLC)." Journal of Applied Biochemistry & Laboratory Medicine 3, no. 2 (2022): 33–34. https://doi.org/10.5005/jablm-11031-03204.

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K., Hari Ramakrishnan, and Janaky Ranjithkumar. "Quantitative Determination of Vitamin E Isomers using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography." Indian Journal of Nutrition and Dietetics 54, no. 3 (2017): 294. http://dx.doi.org/10.21048/ijnd.2017.54.3.15589.

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Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities

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Renger, Bernd. "Contemporary Thin-Layer Chromatography in Pharmaceutical Quality Control." Journal of AOAC INTERNATIONAL 81, no. 2 (1998): 333–40. http://dx.doi.org/10.1093/jaoac/81.2.333.

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abstract The progress in layers, instrumentation, and the development of automated scanning densitometers have led to a remarkable improvement of the features of thin-layer chromatography (TLC) and especially its high performance version (HPTLC). Contemporary TLC or HPTLC combines the techniques' inherent advantages which include high sample throughput, simultaneous processing of standards and samples, flexibility, easy postchro matographic visualization techniques, and the ability to handle complex or crude samples with minimum sample cleanup—with improved selectivity, sensitivity, and accura

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Ajeel, Zaineb H., and Maha N. Hamad. "Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq." Iraqi Journal of Pharmaceutical Sciences ( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512) 29, no. 2 (2020): 202–13. http://dx.doi.org/10.31351/vol29iss2pp202-213.

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The target of this study was to study the natural phytochemical components of the head (capsule) of Cynara scolymus cultivated in Iraq. The head (capsule) of plant was extracted by maceration in70% ethanol for 72 hours, and fractioned by hexane, chloroform and ethyl acetate. Preliminary qualitative phytochemical screening was performed on the ethyl acetate fraction for capsule was revealed the presence of flavonoid and aromatic acids. These were examined by (high -performance liquid chromatography) (HPLC diodarray), (high- performance thin-layer chromatography)(HPTLC). Flavonoids were isolated

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Guermouche, M. H., D. Habel, and S. Guermouche. "Assay of Tinidazole in Human Serum by High-Performance Thin-Layer Chromatography—Comparison with High-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 82, no. 2 (1999): 244–47. http://dx.doi.org/10.1093/jaoac/82.2.244.

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Abstract Determination of tinidazole in human serum by high-performance thin-layer chromatography (HPTLC) is presented. It includes use of 10 × 10 cm plates coated with silica gel 60 and chloroform-acetonitrile-acetic acid (60 + 40 + 2) as mobile phase. Quantitation was performed by densitometry at 320 nm. The linearity (1-10 ng), precision (6%), reproducibility (5%), recovery (96%), and detection limit (1 mg/L) of tinidazole determination by HPTLC were comparable with corresponding method parameters by reversed-phase HPLC. A satisfactory correlation was found between the 2 analytical methods.

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R, Balabhaskar, and Vijayalakshmi K. "HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINT PROFILE OF BAUHINIA TOMENTOSA LINN. LEAVES." Asian Journal of Pharmaceutical and Clinical Research 11, no. 2 (2018): 344. http://dx.doi.org/10.22159/ajpcr.2018.v11i2.23294.

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Objective: Chromatographic fingerprint is an effective method for doing the fingerprinting of a plant species. In this study, high-performance thin-layer chromatography (HPTLC) analysis of Bauhinia tomentosa was done in n-hexane, chloroform, and ethanol extracts.Methods: The extract of leaves was developed using toluene:ethyl acetate:formic acid:glacial acetic acid (7:3:0.1:0.1) for n-hexane, toluene:ethyl acetate:formic acid (6:2:0.5) for chloroform, and chloroform:methanol:formic acid (8:1.5:0.2) for ethanol extract as mobile phase using standard procedures and scanned under ultraviolet at 2

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Gray, Dean E., Dale Messer, Andrew Porter, et al. "Analysis of Flavonol Aglycones and Terpenelactones in Ginkgo biloba Extract: A Comparison of High-Performance Thin-Layer Chromatography and Column High-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 90, no. 5 (2007): 1203–9. http://dx.doi.org/10.1093/jaoac/90.5.1203.

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Abstract Advancements in automated high-performance thin-layer chromatography (HPTLC) have made it feasible to assess its use for the quantitative analysis of marker compounds in botanical preparations. We report here the findings of method comparisons for the terpenelactones and flavonol aglycones by column high-performance liquid chromatography (HPLC) with evaporative light scattering and UV detection, and HPTLC with a scanning densitometer. For the HPTLC assay of terpenelactones, total bilobalide, ginkgolide A, and ginkgolide B consistently achieved &lt;70 of the total determined using

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Komal N. Pawar, Komal N. Pawar, Sandhya P. Kadam Sandhya P. Kadam, and Dr VivekkumarK Redasani Dr.VivekkumarK.Redasani. "A Systematic Review on High Performance Thin Layer Chromatography (HPTLC)." International Journal of Pharmaceutical Research and Applications 10, no. 3 (2025): 402–16. https://doi.org/10.35629/4494-1003402416.

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High-Performance Thin Layer Chromatography (HPTLC) is a sophisticated analytical tool that has transformed the science of separation science through its greater sensitivity, resolution, and reproducibility over the conventional Thin Layer Chromatography (TLC). This systematic review delves into the concept, instrumentation, methodological innovation, and vast applications of HPTLC in pharmaceuticals, herbal drugs, food, forensic science, and biotechnology. The review highlights the most important innovations in automation, detection, derivatization, and hyphenation with mass spectrometry and F

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Bober-Majnusz, Katarzyna, and Alina Pyka-Pająk. "Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed Using Thin-Layer Chromatography and High-Performance Thin-Layer Chromatography." Processes 12, no. 6 (2024): 1153. http://dx.doi.org/10.3390/pr12061153.

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TLC (thin-layer chromatography) and HPTLC (high-performance thin-layer chromatography) in normal (NP) and reversed (RP) phase systems were combined with densitometry to analyze caffeine, propyphenazone, and paracetamol. This work aims to check whether comparable limit of detection (LOD) values can be obtained on TLC and HPTLC plates. Analyses were performed on five (NP) or four (RP) different stationary phases (chromatographic plates), testing, in both cases, three mobile phases. It is shown that by using both TLC and HPTLC plates, it is possible to develop chromatographic conditions that enab

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Edward Raju Gope, Shaik Munnisha Begum, Pujitha Prayer Anisetti, et al. "A Review of Principles, Applications, and Recent Developments in HPTLC and HPLC." Journal of Pharma Insights and Research 2, no. 6 (2024): 056–64. https://doi.org/10.69613/315vge42.

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High-Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC) represent fundamental analytical techniques in modern chemical analysis. These sophisticated separation methods have revolutionized the field of analytical chemistry through their precision, reliability, and versatility. HPTLC, an enhancement of traditional thin-layer chromatography, offers advantages such as minimal sample preparation, simultaneous analysis of multiple samples, and cost-effectiveness. It has found extensive applications in pharmaceutical analysis, natural product research, and

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Erum, Aasiy Ul, Amjad Waheed Yousuf, and Mohd Aslam. "High performance thin layer chromatography analysis of seed extracts of Tudri Surkh (Cheiranthus cheiri)." International Journal of Research in Medical Sciences 11, no. 1 (2022): 139. http://dx.doi.org/10.18203/2320-6012.ijrms20223626.

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Background: To develop the high-performance thin layer chromatography (HPTLC) finger print profile of hydroalcoholic and ethylalcohol extracts of seeds of Tudri Surkh (Cheiranthus cheiri). Methods: Chromatographic technique was used for separation of components from hydroalcoholic and ethylalcohol extracts of seeds and HPTLC was carried out using CAMAG HPTLC system equipped with Linomat V applicator, and WinCATS software.Results: HPTLC profiling of the extract confirm the presence of various phytochemicals. At 640 nm HPTLC finger print of hydroalcoholic extract revealed 16 components with Rf=0

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Patel, Rashmin B., Mrunali R. Patel, Madhira B. Shankar, and Kashyap K. Bhatt. "Simultaneous Determination of Alprazolam and Fluoxetine Hydrochloride in Tablet Formulations by High-Performance Column Liquid Chromatography and High-Performance Thin-Layer Chromatography." Journal of AOAC INTERNATIONAL 92, no. 4 (2009): 1082–88. http://dx.doi.org/10.1093/jaoac/92.4.1082.

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Abstract This paper describes validated HPLC and HPTLC methods for simultaneous determination of alprazolam (ALP) and fluoxetine hydrochloride (FXT) in pure powder and formulation. The HPLC separation was achieved on a Nucleosil C8 column (150 mm length, 4.6 mm id, 5 m particle size) using acetonitrilephosphate buffer pH 5.5 (45 + 55, v/v) as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using acetonetolueneammonia (6.0 + 3.5 + 0.5, v/v/v) as the mobile phase. Quantification in the HPLC m

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Miyazaki, Masao, Azusa Yonesige, Junko Matsuda, Yasuhiro Kuroda, Naoya Kojima, and Akemi Suzuki. "High-Performance Thin-Layer Chromatography/Mass Spectrometry for Rapid Analysis of Neutral Glycosphingolipids." Journal of AOAC INTERNATIONAL 91, no. 5 (2008): 1218–26. http://dx.doi.org/10.1093/jaoac/91.5.1218.

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Abstract Direct coupling of high-performance thin-layer chromatography (HPTLC) to matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MS) was shown to be a reliable and reproducible method to obtain structural information and fundamental properties of glycosphingolipids (GSLs).We report a protocol for the preparation of neutral GSL extracts frommouse tissues and demonstrate the applicability of HPTLC/MS to these preparations. The protocol consists of lipid extraction and ion exchange chromatography followed by a mild alkaline treatment and a revers

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Panchal, Hiral J., and Bhanubhai N. Suhagia. "Simultaneous Determination of Atorvastatin Calcium and Ramipril in Capsule Dosage Forms by High-Performance Liquid Chromatography and High-Performance Thin Layer Chromatography." Journal of AOAC INTERNATIONAL 93, no. 5 (2010): 1450–57. http://dx.doi.org/10.1093/jaoac/93.5.1450.

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Abstract Two simple and accurate methods to determine atorvastatin calcium and ramipril in capsule dosage forms were developed and validated using HPLC and HPTLC. The HPLC separation was achieved on a Phenomenex Luna C18 column (250 ⨯ 4.6 mm id, 5 µm) in the isocratic mode using 0.1% phosphoric acidacetonitrile (38 + 62, v/v), pH 3.5 ± 0.05, mobile phase at a flow rate of 1 mL/min. The retention times were 6.42 and 2.86 min for atorvastatin calcium and ramipril, respectively. Quantification was achieved with a photodiode array detector set at 210 nm over the concentration range of 0.5-5 µg/mL

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Thatipelli, Sujith, Achintya Kumar Mandal, and Shakila Ramachandran. "Chemical Standards and HPTLC Finger Print Profiles of a Siddha Polyherbal Formulation - Kadukkai Legiyam." International Journal of Ayurvedic Medicine 12, no. 4 (2021): 922–26. http://dx.doi.org/10.47552/ijam.v12i4.2188.

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To study physico-chemical, phytochemical and high performance thin layer chromatography of a Siddha drug “Kadukkai Legiyam” (KL). The prepared Kadukkai Legiyam (KL) was prepared as per the standard operating procedures mentioned in literature. Then the drug was subjected to physicochemical parameters, phytochemical screening, thin layer chromatographic photo documentation (TLC), high performance thin layer chromatographic (HPTLC) finger print profile of hexane, chloroform, ethanol and hydro alcohol (1:1) extracts. Different extracts of the drug showed distinct TLC and HPTLC finger print patter

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Vijayan, Resmi. "High-performance Thin-layer Chromatography Fingerprinting Analysis of Punarnavadi Kwatha." International Research Journal of Ayurveda & Yoga 06, no. 11 (2023): 25–29. http://dx.doi.org/10.47223/irjay.2023.61105.

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Introduction: Punarnavadi kwatha is one among the commonly used medicine in Ayurvedic practice, mentioned in Chakradatta in sopha chikitsa. Main indications of Punarnavadi kwatha are Sarvanga sopha (generalised oedema), Udara (ascites), Kasa (cough), Soolam (colicky pain), and Swasa (dyspnoea) associated with Pandu (anaemia). For the purpose of quality assurance and uniformity, scientific verification of Ayurvedic medications is important. Usage of modern analytical techniques helps in the proper authentication of medicines. In the present study, highperformance thin-layer chromatography (HPTL

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A., P. Gupta, M. Gupta M., and Kumar Sushil. "High performance thin layer chromatography of asiaticoside in Centella asiatica." Journal of Indian Chemical Society Vol. 76, Jun 1999 (1999): 321–22. https://doi.org/10.5281/zenodo.5852497.

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Central Institute of Medicinal & Aromatic Plants, P.O. CIMAP, Lucknow-226 015, India <em>Manuscript received 4 December 1998, accepted 11 February 1999</em> A high performance thin layer chromatography method, suitable for mass screening of plant samples for the quantitation of active saponin, asiaticoside, from <em>Centella asiatica</em> has been developed. Quantitation was performed in absorption-reflection mode at 620 nm by scanning the <strong>HPTLC</strong> plate <sup>60</sup>F<sub>254</sub> (mobile phase ethyl acetate-methanol-water, 60 : 12 : 8) after a colour development by vanilli

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Patel, Rashmin B., Madhira B. Shankar, Mrunali R. Patel, and Kashyap K. Bhatt. "Simultaneous Estimation of Acetylsalicylic Acid and Clopidogrel Bisulfate in Pure Powder and Tablet Formulations by High-Performance Column Liquid Chromatography and High-Performance Thin-Layer Chromatography." Journal of AOAC INTERNATIONAL 91, no. 4 (2008): 750–55. http://dx.doi.org/10.1093/jaoac/91.4.750.

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Abstract This paper describes validated high-performance column liquid chromatographic (HPLC) and high-performance thin-layer chromatographic (HPTLC) methods for simultaneous estimation of acetylsalicylic acid (ASA) and clopidogrel bisulfate (CLP) in pure powder and formulations. The HPLC separation was achieved on a Nucleosil C8 column (150 mm length 4.6 mm id, 5m particle size) using acetonitrilephosphate buffer, pH 3.0 (55 + 45, v/v) mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using ethy

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Pandhare, R. B., R. N. Kulkarni, V. K. Deshmukh, P. B. Mohite, and A. R. Pawar. "High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery." Journal of Pharmaceutical and Biological Sciences 9, no. 1 (2021): 7–14. http://dx.doi.org/10.18231/j.jpbs.2021.002.

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HPTLC is an advanced type of planar chromatography used extensively in the recent years for fingerprinting of medicinal plants, products and for screening lichen substances, quantification of active ingredients and herbal drugs, phytochemical and biomedical analysis and also used to check of presence of adulterants in the formulation. It is more sensitive and possible to run more sample in a short period of time, by using small amount of solvent. It is one of the sophisticated instrumental techniques based on the full capabilities of thin layer chromatography. In this paper, a brief discussion

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Qassim, Rabab H., and Enas J. Kadhem. "Phytochemical Investigation and Anti-angiogenic Examination of Iraqi Vigna radiata L. Seeds and Sprouts." Iraqi Journal of Pharmaceutical Sciences ( P-ISSN: 1683 - 3597 , E-ISSN : 2521 - 3512) 29, no. 2 (2020): 37–47. http://dx.doi.org/10.31351/vol29iss2pp37-47.

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Abstract  The objective of this study was to investigate the phytochemical constituents of two different parts of Vigna radiata (seeds and sprouts), and identify their anti angiogenic activity .the goal was achieved by Preliminary qualitative phytochemical screening for crude ethanolic extract of two parts of plant ; rat aorta anti-angiogenesis assay had been conducted for both extracts . isolation , separation and purification of some phytochemical constituents that belong to important groups (flavonoids) from n-butanol fraction extract of Vigna radiata plant had been done in pure

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Wójciak-Kosior, Magdalena, Agnieszka Skalska, Grażyna Matysik, and Magdalena Kryska. "Quantitative Analysis of Phenobarbital in Dosage Form by Thin-Layer Chromatography Combined with Densitometry." Journal of AOAC INTERNATIONAL 89, no. 4 (2006): 995–98. http://dx.doi.org/10.1093/jaoac/89.4.995.

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Abstract In this paper, a high-performance thin-layer chromatography (HPTLC) method combined with densitometry has been described. Chromatography was performed on silica gel Si 60F254 plates using dichloromethaneethyl acetateformic acid (9.5 + 0.5 + 0.1, v/v) mobile phase. This method has been successfully applied for the determination of phenobarbital in pharmaceuticals. Obtained results were comparable with traditionally used column high-performance liquid chromatography (HPLC) methods. For the proposed procedure, linearity (r &gt; 0.999), sensitivity (limit of detection 0.4 g/spot), rec

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Brissoit, Alice, Léo Andruszkow, Enola Roussin, Françoise Lohézic Le-Dévéhat, Marylène Chollet Krugler, and Joël Boustie. "High Performance Thin Layer Chromatography (HPTLC) to profile lichen metabolites." Planta Medica 89, no. 14 (2023): 1401. https://doi.org/10.1055/s-0043-1774198.

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Kapase, Sachin N. "ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF COMBINATION OF ANTI-ASTHMATIC DRUGS MONTELUKAST AND DOXOFYLLINE." Journal of Medical pharmaceutical and allied sciences 11, no. 6 (2021): 86–91. http://dx.doi.org/10.22270/jmpas.v10i6.1940.

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For qualitative and quantitative analysis, various analytical techniques are available such as Ultraviolet (UV) Spectrophotometry, High-performance liquid chromatography (HPLC), High-performance thin layer chromatography (HPTLC). As per literature survey, there are some UV, HPLC, Ultra-Performance Liquid Chromatography (UPLC) and HPTLC analytical methods are developed for Montelukast and Doxofylline individually and in a combination with other drugs too, since yet there are no significant stability studies indicating HPLC method reported for Montelukast and Doxofylline combinations. In the cur

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Ragab, Marwa A. A., Mohamed H. Abdel-Hay, Hytham M. Ahmed, and Sara M. Mohyeldin. "Determination of Ibuprofen and Phenylephrine in Tablets by High-Performance Thin Layer Chromatography and in Plasma by High-Performance Liquid Chromatography with Diode Array Detection." Journal of Chromatographic Science 57, no. 7 (2019): 592–99. http://dx.doi.org/10.1093/chromsci/bmz031.

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Abstract Two chromatographic methods (high performance thin layer chromatography (HPTLC) and high performance liquid chromatography–diode array detector (HPLC-DAD)), were addressed for the analysis of a mixture consisted of phenylephrine hydrochloride and ibuprofen in two forms bulk and their combined dosage form. This binary mixture is considered to be a challenging one as the two drugs differ greatly in their chemical and physical properties. Not only this affects their simultaneous analysis, but also hinders their simultaneous extraction from biological fluids as plasma. That is the reason

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Mackenzie, J. R., and M. Truesdale. "Radial "high-performance" thin-layer chromatography used to assess fetal lung maturity." Clinical Chemistry 36, no. 5 (1990): 728–31. http://dx.doi.org/10.1093/clinchem/36.5.728.

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Abstract A radial "high-performance" thin-layer chromatographic (HPTLC) method is described by which the percentages and ratios of phosphatidylinositol, sphingomyelin, lecithin, phosphatidylethanolamine, phosphatidylglycerol, and dimethyl phosphatidylethanolamine may be determined simultaneously. An additional method for radial HPTLC determination of saturated phosphatidylcholine is described. We report results of application of these methods to greater than 2000 specimens of amniotic fluid from both diabetic and nondiabetic cases.

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Wikara, Tina, Sri Murhandini, Herlina B. Setijanti, and Tepy Usia. "Riset Profil Kromatogram Herba Pegagan Secara High Performance Thin Layer Chromatography (HPTLC)." Proceeding of Mulawarman Pharmaceuticals Conferences 3 (April 26, 2016): 422–29. http://dx.doi.org/10.25026/mpc.v3i2.142.

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Telah dilakukan riset Profil Kromatogram/Fingerprint Centella asiatica (L.) Urb. (herba pegagan) yang bertujuan untuk menghasilkan profil kromatogram herba pegagan secara HPTLC dalam menjamin mutu dan standardisasi obat bahan alam. Pembuatan fingerprint dilakukan secara HPTLC dan sebagai sampel digunakan simplisia yang berasal dari 3 (tiga) daerah yang berasal dari Bogor, Solo and Yogyakarta. Fase diam menggunakan plat HPTLC silika gel 60 F254 sedangkan fase gerak menggunakan diklorometan-metanol-air (14:6:1) dan penampak bercak reagen asam sulfat. Untuk melihat spektrum dari asiatikosid sebag

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Patel, Bhavesh H., Bhanubhai N. Suhagia, Madhabhai M. Patel, and Jignesh R. Patel. "Simultaneous Estimation of Pantoprazole and Domperidone in Pure Powder and a Pharmaceutical Formulation by High-Perfomance Liquid Chromatography and High-Performance Thin-Layer Chromatography Methods." Journal of AOAC INTERNATIONAL 90, no. 1 (2007): 142–46. http://dx.doi.org/10.1093/jaoac/90.1.142.

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Abstract This paper describes validated high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods for the simultaneous estimation of pantoprazole (PANT) and domperidone (DOM) in pure powder and capsule formulations. The HPLC separation was achieved on a Phenomenex C18 column (250 mm id, 4.6 mm, 5 μm) using 0.01 M, 6.5 pH ammonium acetate buffer-methanol-acetonitrile (30 + 40 + 30, v/v/v, pH 7.20) as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel

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Mohammad, Ali, Abdul Moheman, and Gaber El-Desoky. "Amino acid and vitamin determinations by TLC/HPTLC: review of the current state." Open Chemistry 10, no. 3 (2012): 731–50. http://dx.doi.org/10.2478/s11532-012-0019-0.

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AbstractSeveral methods to determine amino acids and vitamins in biological and pharmaceutical samples have been reported. Thin layer chromatography (TLC) finds its place when the relatively costly equipment required by other methods is unavailable. This review covers the 1991–2010 literature on TLC/HPTLC (high performance thin layer chromatography) amino acid and vitamin determinations. It gives an overview of the special features as well as the problems in TLC/HPTLC determinations of amino acids and vitamins. Various chromatographic systems useful in amino acid and vitamin identification, se

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Upton, Roy T. "Use of High-Performance Thin Layer Chromatography by the American Herbal Pharmacopoeia." Journal of AOAC INTERNATIONAL 93, no. 5 (2010): 1349–54. http://dx.doi.org/10.1093/jaoac/93.5.1349.

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Abstract TLC characterizations are among the key identity tests in most pharmacopoeial monographs. Pharmacopoeial standards are typically used by industry as a basis for meeting QC requirements and current good manufacturing practices (cGMPs). TLC is a relatively low-cost, highly versatile tool for developing specifications for raw materials, as well as for the various preparations for which pharmacopoeial standards are created. In addition to its use in the development of identity tests, TLC is a valuable tool for screening plant samples that pharmacopoeias must review in the development of m

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Aditi, Gade* Prasanna Datar. "A Review on Analytical Method Development for Drug Sitagliptin." International Journal of Pharmaceutical Sciences 3, no. 4 (2025): 2713–21. https://doi.org/10.5281/zenodo.15263891.

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The Sitagliptin offer significant therapeutic benefits in managing type 2 diabetes mellitus (T2DM) and associated cardiovascular risks. The development of accurate, precise, and validated analytical methods is crucial for ensuring their efficacy, safety, and regulatory compliance. This review explores various analytical techniques, including High-Performance Liquid Chromatography (HPLC), Reverse Phase HPLC (RP- HPLC), High-Performance Thin Layer Chromatography (HPTLC), Liquid Chromatography-Mass Spectrometry (LC-MS/MS), and UV spectrophotometry, highlighting their advantages, challenges, and v

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Lambri, Milena, Michaël Jourdes, Yves Glories, and Cédric Saucier. "High performance thin layer chromatography (HPTLC) analysis of red wine pigments." Journal of Planar Chromatography – Modern TLC 16, no. 2 (2003): 88–94. http://dx.doi.org/10.1556/jpc.16.2003.2.1.

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Reich, Eike, and Anne Schibli. "A standardized approach to modern high-performance thin-layer chromatography (HPTLC)." Journal of Planar Chromatography – Modern TLC 17, no. 100 (2004): 438–43. http://dx.doi.org/10.1556/jpc.17.2004.6.8.

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Morlock, Gertrud. "ManMohan Srivastava (Ed.): High-performance thin-layer chromatography (HPTLC), 1st ed." Analytical and Bioanalytical Chemistry 401, no. 8 (2011): 2331–32. http://dx.doi.org/10.1007/s00216-011-5299-2.

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Agatonovic-Kustrin, Snezana, and David W. Morton. "The Power of HPTLC-ATR-FTIR Hyphenation in Bioactivity Analysis of Plant Extracts." Applied Sciences 10, no. 22 (2020): 8232. http://dx.doi.org/10.3390/app10228232.

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Given the simplicity in sample preparation and application, thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) as its most enhanced form are commonly used to separate and identify complex mixtures in solution [...]

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A., P. Gupta, M. Gupta M., and Kumar Sushil. "Estimation of tropane alkaloids using high performance thin layer chromatography." Journal of Indian Chemical Society Vol. 76, May 1999 (1999): 277–78. https://doi.org/10.5281/zenodo.5851976.

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Central Institute of Medicinal & Aromatic Plants, Lucknow-226 015, India <em>Manuscript received 17 August 1998, revised 22 December 1998, accepted 25 January 1999</em> A simple high performance thin layer chromatographic method for separation and estimation of tropane alkaloids hyoscyamine and hyoscine is described. The method requires no special sample pretreatment and is suitable for rapid screening of plant material.

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Varghese, Susheel John, and Thengungal Kochupappy Ravi. "Determination of Rosuvastatin and Ezetimibe in a Combined Tablet Dosage Form Using High-Performance Column Liquid Chromatography and High-Performance Thin-Layer Chromatography." Journal of AOAC INTERNATIONAL 93, no. 4 (2010): 1222–27. http://dx.doi.org/10.1093/jaoac/93.4.1222.

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Abstract This paper describes validated HPLC and HPTLC methods for the simultaneous determination of rosuvastatin (ROS) and ezetimibe (EZE) in a combined tablet dosage form. The isocratic RP-HPLC analysis was performed on a Chromolith C18 column (100 6 mm id) using 0.1 (v/v) orthophosphoric acid solution (pH 3.5)acetonitrile (63 + 37, v/v) mobile phase at a flow rate of 1 mL/min at ambient temperature. Quantification was carried out using a photodiode array UV detector at 245 nm over the concentration range of 0.510 g/mL for ROS and EZE. The HPTLC separation was carried out on an aluminum-back

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Wikara, Tina, Anny Sulistiowaty, Sri Murhandini, and Tepy Usia. "Fingerprint Study of Curcuma xanthorrhiza Rhizome by High Performance Thin Layer Chromatography (HPTLC)." Jurnal Jamu Indonesia 1, no. 2 (2016): 9–14. http://dx.doi.org/10.29244/jji.v1i2.13.

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The rhizome of Curcuma xanthorrhiza Roxb is intensively used in Indonesia as traditional medicine. It is widely used for hepatoprotective and anti inflammatory activities. To ensure the quality of its extract, we have studied the fingerprint or phytochemical analysis. This research was aimed to produce a chromatogram profile of the rhizome by HPTLC. The HPTLC fingerprint chromatogram of C. xanthorrhiza rhizome was performed using HPTLC plate of silica gel 60 F254 as the stationary phase and chloroform-methanol (97:3) as the mobile phase. Spot detection was carried out by TLC photo documentary

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Wikara, Tina, Anny Sulistiowaty, Sri Murhandini, and Tepy Usia. "Fingerprint Study of Curcuma xanthorrhiza Rhizome by High Performance Thin Layer Chromatography (HPTLC)." Jurnal Jamu Indonesia 1, no. 2 (2016): 9–14. http://dx.doi.org/10.29244/jjidn.v1i2.30607.

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The rhizome of Curcuma xanthorrhiza Roxb is intensively used in Indonesia as traditional medicine. It is widely used for hepatoprotective and anti inflammatory activities. To ensure the quality of its extract, we have studied the fingerprint or phytochemical analysis. This research was aimed to produce a chromatogram profile of the rhizome by HPTLC. The HPTLC fingerprint chromatogram of C. xanthorrhiza rhizome was performed using HPTLC plate of silica gel 60 F254 as the stationary phase and chloroform-methanol (97:3) as the mobile phase. Spot detection was carried out by TLC photo documentary

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Alqarni, Mohammed H., Faiyaz Shakeel, Wael A. Mahdi, et al. "A Greener Stability-Indicating High-Performance Thin-Layer Chromatography Approach for the Estimation of Topiramate." Materials 15, no. 5 (2022): 1731. http://dx.doi.org/10.3390/ma15051731.

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Despite various reported analytical methods for topiramate (TPM) analysis, greener analytical approaches are scarce in literature. As a consequence, the objective of the current research is to design a normal-phase stability-indicating high-performance thin-layer chromatography (SI-HPTLC) methodology for TPM analysis in marketed tablet dosage forms that is rapid, sensitive, and greener. TPM was derivatized densitometrically and analyzed at 423 nm in visible mode with anisaldehyde-sulfuric acid as the derivatizing agent. The greener SI-HPTLC technique was linear in the 30–1200 ng band−1 range.

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Juszczak, Zovko-Končić, and Tomczyk. "Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives." Biomolecules 9, no. 11 (2019): 731. http://dx.doi.org/10.3390/biom9110731.

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Luteolin is a flavonoid often found in various medicinal plants that exhibits multiple biological effects such as antioxidant, anti-inflammatory and immunomodulatory activity. Commercially available medicinal plants and their preparations containing luteolin are often used in the treatment of hypertension, inflammatory diseases, and even cancer. However, to establish the quality of such preparations, appropriate analytical methods should be used. Therefore, the present paper provides the first comprehensive review of the current analytical methods that were developed and validated for the quan

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Kalyani, Ashok Patil*1 Shailesh B. Patil2. "To Develop And Validate Stability Indicating Assay Of Diloxanide Furoate By Using UV And HPLC And HPTLC Method For Pharmaceutical Dosage Form." International Journal in Pharmaceutical Sciences 2, no. 6 (2024): 498–517. https://doi.org/10.5281/zenodo.11543189.

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The study aimed to establish stability assays for Diloxanide Furoate using both High-Performance Liquid Chromatography (HPLC) and High-Performance Thin-Layer Chromatography (HPTLC) methods, covering crucial aspects of method development and validation. Detailed specifications were provided for the HPLC and HPTLC systems to ensure proper setup and functioning. Chromatographic conditions were optimized, including parameters like stationary phase, mobile phase composition, flow rate, and detection wavelength, to achieve optimal separation and detection of Diloxanide Furoate. The identity of pure

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Despotovic, Vesna, Nemanja Trisovic, Anamarija Mandic, Gordana Uscumlic, and Tatjana Djakovic-Sekulic. "Lipophilicity assessment of some 5,5-disubstituted hydantoins by the means of reversed phase liquid chromatography." Chemical Industry and Chemical Engineering Quarterly 19, no. 1 (2013): 1–6. http://dx.doi.org/10.2298/ciceq120124037d.

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The retention of some 5,5-disubstituted hydantoins was investigated by reversed phase high performance thin-layer chromatography (RP HPTLC) and reversed phase high-pressure liquid chromatography (RP HPLC). The mobile phases were mixtures of methanol-water and acetonitrile-water in various volume fractions. In order to explore and visualize similarities and differences among the investigated compounds and chromatographic system Principal Component Analysis (PCA) was used. Results show that the experimental lipophilicity indices estimated from retention data (RM,W, logkw) and PC1 are directly co

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Tamilselvan R, Tamilselvan R., Senthil kumar, S. K. principal cum Professor Afreen A, et al. "A Review in Analytical Method for Determination of Anti Malarial Agents." International Journal of Pharmaceutical Research and Applications 10, no. 1 (2025): 1006–33. https://doi.org/10.35629/4494-100110061033.

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Malaria is an life threatening infectious caused by a plasmodium parasite transmitted by the bite of infected mosquitoes .Malaria mostly spreads to people through the bites of some infected female Anopheles mosquitoes. Blood transfusion and contaminated needles may also transmit malaria. Left untreated, P. falciparum malaria can progress to severe illness and death within 24 hour This study is a review of various analytical .methods used for the determination of antimalarial agents reported in the literature. This review encompasses methods such as spectrometry (UV), high-performance liquid ch

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Karde, Samiksha, Srishti Jha, Bhushan Pimple, et al. "High-Performance Thin Layer Chromatography Method Development to Estimate Phytoconstituents in Hedychium species." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 13, no. 01 (2023): 367–71. http://dx.doi.org/10.25258/ijddt.13.1.59.

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Objective: The H. coronarium (Zingiberaceae) is a perennial herbaceous plant. Chinese immigrants introduced the H. coronarium species as an ornamental plant in 1888. Its attractive fl owers make it a desirable ornamental plant. The present study aims to evaluate the physiochemical characteristics and development of the (HPTLC) Fingerprint sequence profi le of Hedychium coronarium leaves. Method: Pharmacognostic characteristics and physiochemical parameters were performed according to the method prescribed. The mobile phase confi rmation was completed using by performing (TLC). The development

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Yue, Yongde, Rong Zhang, Wei Fan, and Feng Tang. "High-Performance Thin-Layer Chromatographic Analysis of Selected Organophosphorous Pesticide Residues in Tea." Journal of AOAC INTERNATIONAL 91, no. 5 (2008): 1210–17. http://dx.doi.org/10.1093/jaoac/91.5.1210.

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Abstract The separation of 9 organophosphates (monocrotophos, quinalphos, triazophos, parathion-methyl, isofenphos-methyl, temephos, parathion, phoxim-ethyl, and chlorpyrifos) by high-performance thin-layer chromatography (HPTLC) with automated multiple development was studied. The HPTLC method was developed and validated for analysis of residues of phoxim-ethyl and chlorpyrifos in tea. The sample was extracted with acetonitrile and cleaned up by ENVI-CARB solid-phase extraction. The extract was directly applied as bands to glass-backed silica gel 60F254 HPTLC plates. The plates were developed

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Savalia, Vaibhavi, Jahnavi Padariya, Tushar Koshiya, and Devang Pandya. "Phytochemical Investigation of Alysicarpus vaginalis by Gas Chromatography-mass Spectrometry and High-performance Thin-layer Chromatography." International Journal of Pharmaceutical Sciences and Nanotechnology(IJPSN) 17, no. 2 (2024): 7233–41. http://dx.doi.org/10.37285/ijpsn.2024.17.2.4.

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Introduction: Alysicarpus vaginalis belongs to the Fabaceae family, commonly known as buffalo clover and one-leaf clover. Alysicarpus vaginalis is medicinally used in disease-related kidney, diuretics, leprosy, pulmonary problems, jaundice, skin problems, respiratory difficulties, and as a hepatoprotective. Alysicarpus vaginalis is a medicinally important plant according to our review of literature on pharmacological activities, modern pharmacological activities, ethnomedicinal surveys, and traditional systems. However, very limited research related to phytochemical investigation by chromatogr

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Dawane, Vinars, and M. H. Fulekar. "HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC) FINGERPRINTING PATTERN OF MANGROVE AVICENNIA MARINA." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 4 (2017): 65. http://dx.doi.org/10.22159/ijpps.2017v9i4.16480.

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Objective: An attempt has been made to study phytoconstituents and High-Performance Thin Layer Chromatography (HPTLC) fingerprinting pattern of leaf/stem/root methanol extracts of Avicennia marina.Methods: The phytochemical screening was done by standard biochemical methods and standard optimized HPTLC densitometry determination was performed under two different energy zones [under UV-254 nm and under 540 nm after derivatization at sample size-10 µl, temperature = 25.8±0.3 °C (constant) and relative humidity = 86±1% (constant) in optimized solvent system].Results: Phytochemical analysis confir

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Khokhlova, K. O., O. A. Zdoryk, N. V. Sydora, and V. I. Shatrovska. "Chromatographic Profiles Analysis of Fruits of Crataegus L. Genus by High-Performance Thin-Layer Chromatography." European Pharmaceutical Journal 66, no. 2 (2019): 45–51. http://dx.doi.org/10.2478/afpuc-2019-0020.

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Abstract It was known that hawthorn - Crataegus L. is a polymorphic genus. Two hawthorn species and their hybrids are included in the European Pharmacopoeia, twelve – in Ukrainian pharmacopoeia. Determination of chromatographic profiles of hawthorn fruits species native to Ukraine and other countries that are non-pharmacopoeial, but have sufficient plant raw material base, is essential for quality control of drugs. Aim. To analyze and compare the chromatographic profiles of fruits of 23 Crataegus L. species on phenolic compounds, evaluated by means of high-performance thin-layer chromatography

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Arup, Ulf, Stefan Ekman, Louise Lindblom, and Jan-Eric Mattsson. "High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances." Lichenologist 25, no. 1 (1993): 61–71. http://dx.doi.org/10.1006/lich.1993.1018.

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AbstractHigh performance thin layer chromatography (HPTLC) is a method that can be used for screening lichen substances. It is as simple to use as standard TLC, but has many advantages: It is more sensitive, it is possible to run more samples in a shorter period of time, and the amount of solvent used is much smaller. The material needed and the methods used are described in detail. Horizontal chromatogram development was used. Since two of the solvents used in system B have been substituted, and since the properties of the HPTLC plates are slightly different, our results are not entirely in a

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