Relevant bibliographies by topics / High-pressure liquid chromatography (HPLC) DNA adducts

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Academic literature on the topic 'High-pressure liquid chromatography (HPLC) DNA adducts'

Author: Grafiati
Published: 4 June 2021
Last updated: 9 February 2022

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Journal articles on the topic "High-pressure liquid chromatography (HPLC) DNA adducts"

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Dunn, Bruce P. "Detection of Aromatic DNA Adducts in Human Cells and Fish Liver." Journal of the American College of Toxicology 8, no. 5 (1989): 905–12. http://dx.doi.org/10.3109/10915818909018051.

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Both nuclease PI treatment and reverse-phase high-performance liquid chromatography (HPLC) were used to enrich hydrophobic/bulky DNA adducts in DNA digests. 32P-postlabeling procedures and thin layer chromatography were then used to detect and quantitate aromatic/bulky DNA adducts. For both human and fish DNA from individuals exposed to environmental carcinogens, the nuclease PI and HPLC enrichment procedures generally gave similar results. The bottom sediments of the Buffalo and Detroit rivers are contaminated with polycyclic aromatic hydrocarbon carcinogens, and brown bullheads in these rive
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Martinez, Glaucia Regina, Hulyana Brum, Guilherme Lanzi Sassaki, et al. "Oxidation of 1-N2-etheno-2′-deoxyguanosine by singlet molecular oxygen results in 2′-deoxyguanosine: a pathway to remove exocyclic DNA damage?" Biological Chemistry 399, no. 8 (2018): 859–67. http://dx.doi.org/10.1515/hsz-2017-0337.

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Abstract Exocyclic DNA adducts are considered as potential tools for the study of oxidative stress-related diseases, but an important aspect is their chemical reactivity towards oxidant species. We report here the oxidation of 1-N2-etheno-2′-deoxyguanosine (1,N2-εdGuo) by singlet molecular oxygen (1O2) generated by a non-ionic water-soluble endoperoxide [N,N′-di(2,3-dihydroxypropyl)-1,4-naphthalenedipropanamide endoperoxide (DHPNO2)] and its corresponding oxygen isotopically labeled [18O]-[N,N′-di(2,3-dihydroxypropyl)-1,4- naphthalenedipropanamide endoperoxide (DHPN18O2)], and by photosensitiz
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Friedman, H. S., D. M. Kokkinakis, J. Pluda, et al. "Phase I trial of O6-benzylguanine for patients undergoing surgery for malignant glioma." Journal of Clinical Oncology 16, no. 11 (1998): 3570–75. http://dx.doi.org/10.1200/jco.1998.16.11.3570.

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PURPOSE The major mechanism of resistance to alkylnitrosourea therapy is the DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT), which removes chlorethylation or methylation damage from the O6-position of guanine. O6-benzylguanine (O6-BG) is an AGT substrate that inhibits AGT by suicide inactivation. We conducted a phase I trial to define the presurgical dose required for depletion of tumor AGT activity in patients with malignant glioma. MATERIALS AND METHODS Patients were to be treated 18 hours before craniotomy with intravenous doses that ranged between 40 and 100 mg/m2 given over
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Ifegwu, Clinton, Kayode Osunjaye, Folasade Fashogbon, Kolawole Oke, Afolabi Adeniyi, and Chimezie Anyakora. "Urinary 1-Hydroxypyrene as a Biomarker to Carcinogenic Polycyclic Aromatic Hydrocarbon Exposure." Biomarkers in Cancer 4 (January 2012): BIC.S10065. http://dx.doi.org/10.4137/bic.s10065.

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In order to capture the extent of exposure to polycyclic aromatic hydrocarbons (PAHs), various biomarkers have been employed. The biomarkers employed for PAHs include PAHs genetoxic end points in lymphocytes, urinary metabolites, PAH-DNA adducts, and PAH-Protein adducts. Of these, excretory 1-hydroxypyene, a metabolite of pyrene, has been used extensively as a biological monitoring indicator of exposure to PAHs. This study attempts to assess the level of this biomarker in the body fluid of 68 exposed subjects using high performance liquid chromatography HPLC. The subjects screened included aut
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Redzwan, S. Mohd, R. Jamaluddin, A. M. Mohd Sokhini, et al. "Ultra-high performance liquid chromatographic determination of aflatoxin M1 in urine." World Mycotoxin Journal 8, no. 4 (2015): 405–13. http://dx.doi.org/10.3920/wmj2014.1761.

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The development of analytical methods to detect aflatoxin B1 (AFB1) in foodstuffs and its metabolites in human biological samples is useful for risk assessment. The latter methodology, i.e. the measurement of AFB1 biomarkers, has become important to assess human aflatoxin exposure. AFB1-lysine adduct, AFB1-DNA adduct and urinary aflatoxin M1 (AFM1) are some of the AFB1 biomarkers that can be measured by several analytical methods, such as enzyme-linked immunosorbent assay, radioimmunoassay, and high performance liquid chromatography (HPLC). HPLC coupled to a fluorescence detector is useful and
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Beszterda, Monika, Małgorzata Kasperkowiak, Magdalena Frańska, Sandra Jęziołowska, and Rafał Frański. "Ethoxylated Butoxyethanol-BADGE Adducts—New Potential Migrants from Epoxy Resin Can Coating Material." Materials 14, no. 13 (2021): 3682. http://dx.doi.org/10.3390/ma14133682.

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The acetonitrile extracts of can-coating materials have been analyzed by using high-pressure liquid chromatography/electrospray ionization-mass spectrometry (HPLC/ESI-MS). On the basis of detected ions [M + H]+, [M + NH4]+, [M + Na]+ and product ions, the ethoxylated butoxyethanol-bisphenol A diglycidyl ether adducts were identified in two of the analyzed extracts. Although the oxyethylene unit-containing compounds are widely used for the production of different kinds of materials, the ethoxylated species have not been earlier detected in epoxy resin can-coatings.
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Dell’Anno, A., M. Fabiano, G. C. A. Duineveld, A. Kok, and R. Danovaro. "Nucleic Acid (DNA, RNA) Quantification and RNA/DNA Ratio Determination in Marine Sediments: Comparison of Spectrophotometric, Fluorometric, and HighPerformance Liquid Chromatography Methods and Estimation of Detrital DNA." Applied and Environmental Microbiology 64, no. 9 (1998): 3238–45. http://dx.doi.org/10.1128/aem.64.9.3238-3245.1998.

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ABSTRACT In this study, we compared three methods for extraction and quantification of RNA and DNA from marine sediments: (i) a spectrophotometric method using the diphenylamine assay; (ii) a fluorometric method utilizing selective fluorochromes (thiazole orange for total nucleic acids and Hoechst 33258 for DNA); and (iii) a high-pressure liquid chromatography (HPLC) method which uses a specific column to separate RNA and DNA and UV absorption of the nucleic acids for quantification. Sediment samples were collected in the oligotrophic Cretan Sea (eastern Mediterranean, from 40 to 1,540 m in de
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Vanden Bussche, J., S. A. Moore, F. Pasmans, G. G. C. Kuhnle, and L. Vanhaecke. "An approach based on ultra-high pressure liquid chromatography–tandem mass spectrometry to quantify O6-methyl and O6-carboxymethylguanine DNA adducts in intestinal cell lines." Journal of Chromatography A 1257 (September 2012): 25–33. http://dx.doi.org/10.1016/j.chroma.2012.07.040.

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Talaska, Glenn, Kenneth L. Dooley, and Fred F. Kadlubar. "Detection and characterization of carcinogen—DNA adducts in exfoliated urothelial cells from 4-aminobiphenyl-treated dogs by 32P-postlabelling and subsequent thin-layer and high-pressure liquid chromatography." Carcinogenesis 11, no. 4 (1990): 639–46. http://dx.doi.org/10.1093/carcin/11.4.639.

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Masson, Luke, Martin Erlandson, Marianne Puzstai-Carey, Roland Brousseau, Victor Juárez-Pérez, and Roger Frutos. "A Holistic Approach for Determining the Entomopathogenic Potential of Bacillus thuringiensisStrains." Applied and Environmental Microbiology 64, no. 12 (1998): 4782–88. http://dx.doi.org/10.1128/aem.64.12.4782-4788.1998.

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ABSTRACT The cry gene content of Bacillus thuringiensis subsp. aizawai HD-133 was analyzed by a combination of high-pressure liquid chromatography (HPLC) and exclusive PCR. A total of six cry genes were detected in genomic DNA purified from HD-133, four from the cry1 family (cry1Aa, cry1Ab, cry1C, andcry1D) as well as a gene each from the cry2(cry2B) and the cry1I families. To directly determine which genes were expressed and crystallized in the purified parasporal inclusions, solubilized and trypsinized HD-133 crystals were subjected to chromatographic separation by HPLC. Only three proteins,
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Dissertations / Theses on the topic "High-pressure liquid chromatography (HPLC) DNA adducts"

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Čechová, Tereza. "Studium metabolismu vzdušných polutantů a mutagenů 3-nitrobenzanthronu a 2-nitrobenzanthronu." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-308300.

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Nitroaromatic compounds are mutagenic and carcinogenic substances present in environment. Most of nitroaromatic compounds are potent mutagens in bacterial and mammalian systems. They are also carcinogens causing development of tumors, primarily in the liver, lung and mammary glands. 3-Nitrobenzanthrone (3-NBA, 3-nitro-7H-benz [de] anthracene-7-one) is one of the polycyclic aromatic nitro compounds possesing high toxic effects. 3-NBA is an environmental pollutant present in diesel exhaust and was also detected in soil and in rain water. 2-Nitrobenzanthrone (2-NBA, 2-nitro-7H-benz [de] anthracen
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