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Journal articles on the topic 'High-pressure liquid chromatography (HPLC) DNA adducts'

Author: Grafiati
Published: 4 June 2021
Last updated: 9 February 2022

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1

Dunn, Bruce P. "Detection of Aromatic DNA Adducts in Human Cells and Fish Liver." Journal of the American College of Toxicology 8, no. 5 (1989): 905–12. http://dx.doi.org/10.3109/10915818909018051.

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Both nuclease PI treatment and reverse-phase high-performance liquid chromatography (HPLC) were used to enrich hydrophobic/bulky DNA adducts in DNA digests. 32P-postlabeling procedures and thin layer chromatography were then used to detect and quantitate aromatic/bulky DNA adducts. For both human and fish DNA from individuals exposed to environmental carcinogens, the nuclease PI and HPLC enrichment procedures generally gave similar results. The bottom sediments of the Buffalo and Detroit rivers are contaminated with polycyclic aromatic hydrocarbon carcinogens, and brown bullheads in these rive
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2

Martinez, Glaucia Regina, Hulyana Brum, Guilherme Lanzi Sassaki, et al. "Oxidation of 1-N2-etheno-2′-deoxyguanosine by singlet molecular oxygen results in 2′-deoxyguanosine: a pathway to remove exocyclic DNA damage?" Biological Chemistry 399, no. 8 (2018): 859–67. http://dx.doi.org/10.1515/hsz-2017-0337.

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Abstract Exocyclic DNA adducts are considered as potential tools for the study of oxidative stress-related diseases, but an important aspect is their chemical reactivity towards oxidant species. We report here the oxidation of 1-N2-etheno-2′-deoxyguanosine (1,N2-εdGuo) by singlet molecular oxygen (1O2) generated by a non-ionic water-soluble endoperoxide [N,N′-di(2,3-dihydroxypropyl)-1,4-naphthalenedipropanamide endoperoxide (DHPNO2)] and its corresponding oxygen isotopically labeled [18O]-[N,N′-di(2,3-dihydroxypropyl)-1,4- naphthalenedipropanamide endoperoxide (DHPN18O2)], and by photosensitiz
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3

Friedman, H. S., D. M. Kokkinakis, J. Pluda, et al. "Phase I trial of O6-benzylguanine for patients undergoing surgery for malignant glioma." Journal of Clinical Oncology 16, no. 11 (1998): 3570–75. http://dx.doi.org/10.1200/jco.1998.16.11.3570.

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PURPOSE The major mechanism of resistance to alkylnitrosourea therapy is the DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT), which removes chlorethylation or methylation damage from the O6-position of guanine. O6-benzylguanine (O6-BG) is an AGT substrate that inhibits AGT by suicide inactivation. We conducted a phase I trial to define the presurgical dose required for depletion of tumor AGT activity in patients with malignant glioma. MATERIALS AND METHODS Patients were to be treated 18 hours before craniotomy with intravenous doses that ranged between 40 and 100 mg/m2 given over
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4

Ifegwu, Clinton, Kayode Osunjaye, Folasade Fashogbon, Kolawole Oke, Afolabi Adeniyi, and Chimezie Anyakora. "Urinary 1-Hydroxypyrene as a Biomarker to Carcinogenic Polycyclic Aromatic Hydrocarbon Exposure." Biomarkers in Cancer 4 (January 2012): BIC.S10065. http://dx.doi.org/10.4137/bic.s10065.

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In order to capture the extent of exposure to polycyclic aromatic hydrocarbons (PAHs), various biomarkers have been employed. The biomarkers employed for PAHs include PAHs genetoxic end points in lymphocytes, urinary metabolites, PAH-DNA adducts, and PAH-Protein adducts. Of these, excretory 1-hydroxypyene, a metabolite of pyrene, has been used extensively as a biological monitoring indicator of exposure to PAHs. This study attempts to assess the level of this biomarker in the body fluid of 68 exposed subjects using high performance liquid chromatography HPLC. The subjects screened included aut
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5

Redzwan, S. Mohd, R. Jamaluddin, A. M. Mohd Sokhini, et al. "Ultra-high performance liquid chromatographic determination of aflatoxin M1 in urine." World Mycotoxin Journal 8, no. 4 (2015): 405–13. http://dx.doi.org/10.3920/wmj2014.1761.

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The development of analytical methods to detect aflatoxin B1 (AFB1) in foodstuffs and its metabolites in human biological samples is useful for risk assessment. The latter methodology, i.e. the measurement of AFB1 biomarkers, has become important to assess human aflatoxin exposure. AFB1-lysine adduct, AFB1-DNA adduct and urinary aflatoxin M1 (AFM1) are some of the AFB1 biomarkers that can be measured by several analytical methods, such as enzyme-linked immunosorbent assay, radioimmunoassay, and high performance liquid chromatography (HPLC). HPLC coupled to a fluorescence detector is useful and
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6

Beszterda, Monika, Małgorzata Kasperkowiak, Magdalena Frańska, Sandra Jęziołowska, and Rafał Frański. "Ethoxylated Butoxyethanol-BADGE Adducts—New Potential Migrants from Epoxy Resin Can Coating Material." Materials 14, no. 13 (2021): 3682. http://dx.doi.org/10.3390/ma14133682.

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The acetonitrile extracts of can-coating materials have been analyzed by using high-pressure liquid chromatography/electrospray ionization-mass spectrometry (HPLC/ESI-MS). On the basis of detected ions [M + H]+, [M + NH4]+, [M + Na]+ and product ions, the ethoxylated butoxyethanol-bisphenol A diglycidyl ether adducts were identified in two of the analyzed extracts. Although the oxyethylene unit-containing compounds are widely used for the production of different kinds of materials, the ethoxylated species have not been earlier detected in epoxy resin can-coatings.
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7

Dell’Anno, A., M. Fabiano, G. C. A. Duineveld, A. Kok, and R. Danovaro. "Nucleic Acid (DNA, RNA) Quantification and RNA/DNA Ratio Determination in Marine Sediments: Comparison of Spectrophotometric, Fluorometric, and HighPerformance Liquid Chromatography Methods and Estimation of Detrital DNA." Applied and Environmental Microbiology 64, no. 9 (1998): 3238–45. http://dx.doi.org/10.1128/aem.64.9.3238-3245.1998.

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ABSTRACT In this study, we compared three methods for extraction and quantification of RNA and DNA from marine sediments: (i) a spectrophotometric method using the diphenylamine assay; (ii) a fluorometric method utilizing selective fluorochromes (thiazole orange for total nucleic acids and Hoechst 33258 for DNA); and (iii) a high-pressure liquid chromatography (HPLC) method which uses a specific column to separate RNA and DNA and UV absorption of the nucleic acids for quantification. Sediment samples were collected in the oligotrophic Cretan Sea (eastern Mediterranean, from 40 to 1,540 m in de
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8

Vanden Bussche, J., S. A. Moore, F. Pasmans, G. G. C. Kuhnle, and L. Vanhaecke. "An approach based on ultra-high pressure liquid chromatography–tandem mass spectrometry to quantify O6-methyl and O6-carboxymethylguanine DNA adducts in intestinal cell lines." Journal of Chromatography A 1257 (September 2012): 25–33. http://dx.doi.org/10.1016/j.chroma.2012.07.040.

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9

Talaska, Glenn, Kenneth L. Dooley, and Fred F. Kadlubar. "Detection and characterization of carcinogen—DNA adducts in exfoliated urothelial cells from 4-aminobiphenyl-treated dogs by 32P-postlabelling and subsequent thin-layer and high-pressure liquid chromatography." Carcinogenesis 11, no. 4 (1990): 639–46. http://dx.doi.org/10.1093/carcin/11.4.639.

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10

Masson, Luke, Martin Erlandson, Marianne Puzstai-Carey, Roland Brousseau, Victor Juárez-Pérez, and Roger Frutos. "A Holistic Approach for Determining the Entomopathogenic Potential of Bacillus thuringiensisStrains." Applied and Environmental Microbiology 64, no. 12 (1998): 4782–88. http://dx.doi.org/10.1128/aem.64.12.4782-4788.1998.

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ABSTRACT The cry gene content of Bacillus thuringiensis subsp. aizawai HD-133 was analyzed by a combination of high-pressure liquid chromatography (HPLC) and exclusive PCR. A total of six cry genes were detected in genomic DNA purified from HD-133, four from the cry1 family (cry1Aa, cry1Ab, cry1C, andcry1D) as well as a gene each from the cry2(cry2B) and the cry1I families. To directly determine which genes were expressed and crystallized in the purified parasporal inclusions, solubilized and trypsinized HD-133 crystals were subjected to chromatographic separation by HPLC. Only three proteins,
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11

Bultreys, Alain, Isabelle Gheysen, and Edmond de Hoffmann. "Yersiniabactin Production by Pseudomonas syringae and Escherichia coli, and Description of a Second Yersiniabactin Locus Evolutionary Group." Applied and Environmental Microbiology 72, no. 6 (2006): 3814–25. http://dx.doi.org/10.1128/aem.00119-06.

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ABSTRACT The siderophore and virulence factor yersiniabactin is produced by Pseudomonas syringae. Yersiniabactin was originally detected by high-pressure liquid chromatography (HPLC); commonly used PCR tests proved ineffective. Yersiniabactin production in P. syringae correlated with the possession of irp1 located in a predicted yersiniabactin locus. Three similarly divergent yersiniabactin locus groups were determined: the Yersinia pestis group, the P. syringae group, and the Photorhabdus luminescens group; yersiniabactin locus organization is similar in P. syringae and P. luminescens. In P.
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12

Sugiono, Erizal, Andi Wijaya, Anwar Santoso, Ferry Sandra, Ilham Jaya Patellongi, and Irawan Yusuf. "Frequencies of CYP1A2 Single Nucleotide Polymorphism in Indonesian and Its Effect on Blood Pressure." Indonesian Biomedical Journal 10, no. 3 (2018): 297–302. http://dx.doi.org/10.18585/inabj.v10i3.374.

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BACKGROUND: The association between caffeine with blood pressure (BP) still remains controversial. Caffeine is mainly metabolized by cytochrome-P450 (CYP)1A2 enzyme. Polymorphism of CYP1A2 is known to cause interindividual variation on enzymatic activity, thus affects caffeine metabolism and its effect on cardiovascular (CV) system.METHODS: We conducted a cross-sectional study and recruited 121 Indonesian subjects aged 25-60 years with varying coffee-drinking habits. DNA was extracted from peripheral blood and genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR
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13

Yang, Ming-Ming, Dmitri V. Mavrodi, Olga V. Mavrodi, et al. "Biological Control of Take-All by Fluorescent Pseudomonas spp. from Chinese Wheat Fields." Phytopathology® 101, no. 12 (2011): 1481–91. http://dx.doi.org/10.1094/phyto-04-11-0096.

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Take-all disease of wheat caused by the soilborne fungus Gaeumannomyces graminis var. tritici is one of the most important root diseases of wheat worldwide. Bacteria were isolated from winter wheat from irrigated and rainfed fields in Hebei and Jiangsu provinces in China, respectively. Samples from rhizosphere soil, roots, stems, and leaves were plated onto King's medium B agar and 553 isolates were selected. On the basis of in vitro tests, 105 isolates (19% of the total) inhibited G. graminis var. tritici and all were identified as Pseudomonas spp. by amplified ribosomal DNA restriction analy
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14

JAMES, PETER. "Protein identification in the post-genome era: the rapid rise of proteomics." Quarterly Reviews of Biophysics 30, no. 4 (1997): 279–331. http://dx.doi.org/10.1017/s0033583597003399.

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Most advances in biology can usually be traced back to the development of a new technique: the recent explosion in sequence information in the databases arose from the pioneering work on separation methods by Frederick Sanger which paved the way for the development of protein (Sanger, 1945) and DNA/RNA (Maxam & Gilbert, 1977; Sanger, 1981) sequencing and culminated in the receipt of two Nobel prizes by Sanger. The initial phase of sequence database expansion was slow due to the tedious and slow nature of protein sequencing. Peptide sequencing was carried out manually and the complete analy
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15

Saez, R., J. B. Craig, J. G. Kuhn, et al. "Phase I clinical investigation of amonafide." Journal of Clinical Oncology 7, no. 9 (1989): 1351–58. http://dx.doi.org/10.1200/jco.1989.7.9.1351.

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Amonafide (benzisoquinolinedione, NSC 308847) is a new synthetic imide antineoplastic agent with DNA intercalative properties that has been evaluated in a phase I clinical trial. The drug was administered as a single intravenous (IV) infusion over 30 to 120 minutes repeated every 28 days. Ninety-five courses of therapy at doses ranging from 18 to 1,104 mg/m2 were administered to 38 patients with refractory solid tumors. Granulocytopenia was dose limiting. Leukopenia was seen in 13 of 31 courses at doses of 690 mg/m2 or greater. Life-threatening granulocytopenia (less than or equal to 250 micro
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16

Weiss-Wichert, Ch, M. Smetazko, M. Valina-Saba, and Th Schalkhammer. "A New Analytical Device Based on Gated Ion Channels: A Peptide-Channel Biosensor." Journal of Biomolecular Screening 2, no. 1 (1997): 11–18. http://dx.doi.org/10.1177/108705719700200104.

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Binding of a protein at a ligand-modified ion channel can lead to closure or distortion of the channel, resulting in a digital on/off response of the ion flux. This principle could be applied as a strategy for sensor design using artificial membrane ligands or receptors. Important details of modeling and a technique of realization of the new molecular electronic sensor device are presented. The response of the sensor induced by analyte binding depends on the analyte's size and ability to close or distort the ion channel. Testing different ion channels in a typical single molecule-binding event
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17

Patel, Rajeshri D., Mihir K. Raval, and Trupesh M. Pethani. "Application of a Validated RP-HPLC Method in Solubility and Dissolution Testing for Simultaneous Estimation of Diacerein and Its Active Metabolite Rhein in Presence of Coformers in the Eutectic Tablet Formulation." Journal of Chromatographic Science, December 15, 2020. http://dx.doi.org/10.1093/chromsci/bmaa109.

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Abstract The present study aimed to develop and validate a novel reversed-phase high-performance liquid chromatography method for simultaneous estimation of Diacerein (DIA) and Rhein (Rh, alkaline degradation product and active metabolite) in the presence of various coformers used to prepare eutectic oral formulation. Chromatographic separations were achieved on a Phenomenex Gemini C18 column (250 mm × 4.6 mm, 5 μm) placed in the thermostated column oven at 40°C. The mobile phase, comprising of acetonitrile and 10 mM ammonium acetate (pH 3.0), was eluted through the gradient system with 0.8 mL
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18

MIKEŠ, J., J. ŠEVC, J. KOŠUTH, A. MATIAŠOVÁ, Z. DAXNEROVÁ, and P. FEDOROČKO. "Flow Cytometric Method for Estimation of 5-bromo-2′-deoxyuridine Content in Rat Serum." Physiological Research, December 17, 2014, 763–70. http://dx.doi.org/10.33549/physiolres.932753.

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Labelling of DNA in replicating cells using 5-bromo-2′-deoxyuridine (BrdU) is widely used, however the rapid clearance and metabolisation of BrdU in the living organism is a critical issue. Although the pharmacokinetic of BrdU in experimental animals is empirically approximated, the exact time-curve remains unknown. Here we present novel method for estimation of the BrdU content in the blood serum. The application is based on the in vitro cocultivation of tumour cells with the examined serum and the subsequent quantification of the incorporated BrdU in the DNA using flow cytometry analysis. Ou
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