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Journal articles on the topic 'High resolution melting analysis'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Carillo, Serge, Laurent Henry, Eric Lippert, et al. "Nested High-Resolution Melting Curve Analysis." Journal of Molecular Diagnostics 13, no. 3 (2011): 263–70. http://dx.doi.org/10.1016/j.jmoldx.2010.12.002.

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2

Erali, Maria, and Carl T. Wittwer. "High resolution melting analysis for gene scanning." Methods 50, no. 4 (2010): 250–61. http://dx.doi.org/10.1016/j.ymeth.2010.01.013.

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3

Tong, S. Y. C., and P. M. Giffard. "Microbiological Applications of High-Resolution Melting Analysis." Journal of Clinical Microbiology 50, no. 11 (2012): 3418–21. http://dx.doi.org/10.1128/jcm.01709-12.

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4

Ro, Na Young, On Sook Hur, Ho Cheol Ko, et al. "Evaluation of Resistance in Pepper Germplasm to Cucumber mosaic virus by High Resolution Melting Analysis." Research in Plant Disease 18, no. 4 (2012): 290–97. http://dx.doi.org/10.5423/rpd.2012.18.4.290.

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5

Wittwer, Carl T., Gudrun H. Reed, Cameron N. Gundry, Joshua G. Vandersteen, and Robert J. Pryor. "High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen." Clinical Chemistry 49, no. 6 (2003): 853–60. http://dx.doi.org/10.1373/49.6.853.

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Abstract Background: High-resolution amplicon melting analysis was recently introduced as a closed-tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003;49:396–406). The technique required a fluorescently labeled primer and was limited to the detection of mutations residing in the melting domain of the labeled primer. Our aim was to develop a closed-tube system for genotyping and mutation scanning that did not require labeled oligonucleotides. Methods: We studied polymorphisms in the hydroxytryptamine receptor 2A (HTR2A) gene (T102C), β-globin (hemoglobins S and C) gen

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6

Zambounis, Antonios, Eleni Stefanidou, Panagiotis Madesis, Jovana Hrustić, Milica Mihajlović, and Brankica Tanović. "Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis." Plant Protection Science 57, No. 1 (2020): 38–46. http://dx.doi.org/10.17221/35/2020-pps.

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Monilinia laxa, Monilinia fructicola and Monilinia fructigena are the three main causal agents of brown rot, which is one of the most important diseases of stone fruits in pre- and postharvest conditions. Nowadays, the need for the precise genotyping of these Monilinia species in terms of the genetic diversity of their populations or differences in their pathogenicity and host range is a prerequisite for any efficient disease management. In our study, the genetic structure of Monilinia populations in Serbia from three geographically distinct regions was investigated employing <br /> a hi

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7

Simko, Ivan. "High-Resolution DNA Melting Analysis in Plant Research." Trends in Plant Science 21, no. 6 (2016): 528–37. http://dx.doi.org/10.1016/j.tplants.2016.01.004.

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8

Mader, Eduard, Joana Ruzicka, Corinna Schmiderer, and Johannes Novak. "Quantitative high-resolution melting analysis for detecting adulterations." Analytical Biochemistry 409, no. 1 (2011): 153–55. http://dx.doi.org/10.1016/j.ab.2010.10.009.

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9

Janavicius, Ramunas, Dovile Matiukaite, Arturas Jakubauskas, and Laimonas Griskevicius. "Microsatellite Instability Detection by High-Resolution Melting Analysis." Clinical Chemistry 56, no. 11 (2010): 1750–57. http://dx.doi.org/10.1373/clinchem.2010.150680.

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BACKGROUND Microsatellite instability (MSI) is an important marker for screening for hereditary nonpolyposis colorectal cancer (Lynch syndrome) as well as a prognostic and predictive marker for sporadic colorectal cancer (CRC). The mononucleotide microsatellite marker panel is a well-established and superior alternative to the traditional Bethesda MSI analysis panel, and does not require testing for corresponding normal DNA. The most common MSI detection techniques—fluorescent capillary electrophoresis and denaturing HPLC (DHPLC)—both have advantages and drawbacks. A new high-resolution meltin

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10

Wittwer, Carl T. "High-resolution DNA melting analysis: advancements and limitations." Human Mutation 30, no. 6 (2009): 857–59. http://dx.doi.org/10.1002/humu.20951.

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11

Laurie, Andrew D., Mark P. Smith, and Peter M. George. "Detection of Factor VIII Gene Mutations by High-Resolution Melting Analysis." Clinical Chemistry 53, no. 12 (2007): 2211–14. http://dx.doi.org/10.1373/clinchem.2007.093781.

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Abstract Background: Single base-pair substitution mutations in the gene for coagulation factor VIII, procoagulant component (hemophilia A) (F8) account for approximately 50% of severe cases of hemophilia A (HA), and almost all moderate or mild cases. Because F8 is a large gene, mutation screening using denaturing HPLC or DNA sequencing is time-consuming and expensive. Methods: We evaluated high-resolution melting analysis as an option for screening for F8 gene mutations. The melting curves of amplicons heterozygous for known F8 gene mutations were compared with melting curves of the correspon

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12

Zumaraga, Mark Pretzel, Marietta Rodriguez, Vanessa Joy Timoteo, and Celeste Tanchoco. "Method Validation of a High Resolution Melting Analysis of a Candidate Genetic Marker of Hypertension." Journal of the ASEAN Federation of Endocrine Societies 30, no. 1 (2015): 18–24. http://dx.doi.org/10.15605/jafes.030.01.01.

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13

Cho, Michael H., Dawn Ciulla, Barbara J. Klanderman, Benjamin A. Raby, and Edwin K. Silverman. "High-Resolution Melting Curve Analysis of Genomic and Whole-Genome Amplified DNA." Clinical Chemistry 54, no. 12 (2008): 2055–58. http://dx.doi.org/10.1373/clinchem.2008.109744.

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Abstract Background: High-resolution melting curve analysis is an accurate method for mutation detection in genomic DNA. Few studies have compared the performance of high-resolution DNA melting curve analysis (HRM) in genomic and whole-genome amplified (WGA) DNA. Methods: In 39 paired genomic and WGA samples, 23 amplicons from 9 genes were PCR amplified and analyzed by high-resolution melting curve analysis using the 96-well LightScanner (Idaho Technology). We used genotyping and bidirectional resequencing to verify melting curve results. Results: Melting patterns were concordant between the g

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14

Shestakova, Anna, Felipe Lorenzo, Tsewang Tashi, Lucie Lanikova, Carl T. Wittwer, and Josef T. Prchal. "Tibetan PHD2D4E High Altitude Adapted Gene Can be Rapidly Detected By High Resolution Melting Assay." Blood 124, no. 21 (2014): 4875. http://dx.doi.org/10.1182/blood.v124.21.4875.4875.

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Abstract High altitude is accompanied by hypoxia. Acute and chronic hypoxia induces a number of compensatory physiological responses mediated by hypoxia-inducible factors (HIFs) that regulate erythropoiesis, iron and energy metabolism, and other essential organismal responses. Excessive HIF responses occurring at high altitude may be accompanied by morbidity (polycythemia and pulmonary hypertension) or mortality (brain and pulmonary edema). HIFs are down regulated by two principal factors, i.e. prolyl hydroxylases (PHDs) and von Hippel Lindau proteins (VHL). Tibetans have lived at 3,000-5,000

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15

Knopkiewicz, M., M. Gawłowska, and W. Święcicki. "The application of high resolution melting in the analysis of simple sequence repeat and single nucleotide polymorphism markers in a pea (Pisum sativum L.) population." Czech Journal of Genetics and Plant Breeding 50, No. 2 (2014): 151–56. http://dx.doi.org/10.17221/113/2013-cjgpb.

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The aim of this study was to verify the high resolution melting (HRM) method in the analysis of single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) markers in pea (Pisum sativum L.). A recombinant inbred line population, Carneval &times; MP1401, was tested for three SNP and 103 SSR markers. HRM analysis was conducted on a LightScanner 96 instrument with LC Green dye. The melting curve shape permitted two polymorphic genotypes to be distinguished. The results were confirmed by gel electrophoresis. Three SSR markers were sequenced and analysed by the melting prediction soft

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16

Li, Huaizhong, Ruiting Lan, Niancai Peng, Jing Sun, and Yong Zhu. "High resolution melting curve analysis with MATLAB-based program." Measurement 90 (August 2016): 178–86. http://dx.doi.org/10.1016/j.measurement.2016.04.057.

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17

Schmiderer, Corinna, Eduard Mader, and Johannes Novak. "DNA-Based Identification ofHelleborus nigerby High-Resolution Melting Analysis." Planta Medica 76, no. 16 (2010): 1934–37. http://dx.doi.org/10.1055/s-0030-1249908.

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18

Provaznikova, Dana, Tereza Kumstyrova, Roman Kotlin, et al. "High-resolution melting analysis for detection of MYH9 mutations." Platelets 19, no. 6 (2008): 471–75. http://dx.doi.org/10.1080/09537100802140013.

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19

Slany, Michal, Martina Vanerkova, Eva Nemcova, Barbora Zaloudikova, Filip Ruzicka, and Tomas Freiberger. "Differentiation of Staphylococcus spp. by high-resolution melting analysis." Canadian Journal of Microbiology 56, no. 12 (2010): 1040–49. http://dx.doi.org/10.1139/w10-091.

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High-resolution melting analysis (HRMA) is a fast (post-PCR) high-throughput method to scan for sequence variations in a target gene. The aim of this study was to test the potential of HRMA to distinguish particular bacterial species of the Staphylococcus genus even when using a broad-range PCR within the 16S rRNA gene where sequence differences are minimal. Genomic DNA samples isolated from 12 reference staphylococcal strains ( Staphylococcus aureus , Staphylococcus capitis , Staphylococcus caprae , Staphylococcus epidermidis , Staphylococcus haemolyticus , Staphylococcus hominis , Staphyloco

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20

Rojo, Daniela, Manuel Zapata, Alejandro Maureira, Ricardo Guiñez, Cristian Wulff-Zottele, and Mariella Rivas. "High-resolution melting analysis for identification of microalgae species." Journal of Applied Phycology 32, no. 6 (2020): 3901–11. http://dx.doi.org/10.1007/s10811-020-02240-y.

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21

Vandersteen, Joshua G., Pinar Bayrak-Toydemir, Robert A. Palais, and Carl T. Wittwer. "Identifying Common Genetic Variants by High-Resolution Melting." Clinical Chemistry 53, no. 7 (2007): 1191–98. http://dx.doi.org/10.1373/clinchem.2007.085407.

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Abstract Background: Heteroduplex scanning techniques usually detect all heterozygotes, including common variants not of clinical interest. Methods: We conducted high-resolution melting analysis on the 24 exons of the ACVRL1 and ENG genes implicated in hereditary hemorrhagic telangiectasia (HHT). DNA in samples from 13 controls and 19 patients was PCR amplified in the presence of LCGreen® I, and all 768 exons melted in an HR-1® instrument. We used 10 wild-type controls to identify common variants, and the remaining samples were blinded, amplified, and analyzed by melting curve normalization an

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22

Antonios, Zambounis, Samaras Anastasios, Xanthopoulou Aliki, et al. "Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation." Plant Protection Science 52, No. 3 (2016): 176–81. http://dx.doi.org/10.17221/179/2015-pps.

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23

Margraf, Rebecca L., Rong Mao, W. Edward Highsmith, Leonard M. Holtegaard, and Carl T. Wittwer. "Mutation Scanning of the RET Protooncogene Using High-Resolution Melting Analysis." Clinical Chemistry 52, no. 1 (2006): 138–41. http://dx.doi.org/10.1373/clinchem.2005.052951.

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Abstract Background: Single-base pair missense mutations in exons 10, 11, 13, 14, 15, and 16 of the RET protooncogene are associated with the autosomal dominant multiple endocrine neoplasia type 2 (MEN2) syndromes: MEN2A, MEN2B, and familial medullary thyroid carcinoma. The current widely used approach for RET mutation detection is sequencing of the exons. Methods: Because RET mutations are rare and the majority are heterozygous mutations, we investigated RET mutation detection by high-resolution amplicon melting analysis. This mutation scanning technique uses a saturating double-stranded nucl

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24

Zhou, Luming, Lesi Wang, Robert Palais, Robert Pryor, and Carl T. Wittwer. "High-Resolution DNA Melting Analysis for Simultaneous Mutation Scanning and Genotyping in Solution." Clinical Chemistry 51, no. 10 (2005): 1770–77. http://dx.doi.org/10.1373/clinchem.2005.054924.

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Abstract Background: High-resolution DNA melting analysis with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probes has been reported. However, simultaneous PCR product scanning and probe genotyping in the same reaction has not been described. Methods: Asymmetric PCR was performed in the presence of unlabeled oligonucleotide probes and a saturating fluorescent DNA dye. High-resolution melting curves for samples in either capillaries (0.3 °C/s) or microtiter format (0.1 °C/s) were generated in the same containers used for amplification. Melting curves

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25

Cheng, Ju-Chien, Chien-Ling Huang, Chung-Ching Lin, et al. "Rapid Detection and Identification of Clinically Important Bacteria by High-Resolution Melting Analysis after Broad-Range Ribosomal RNA Real-Time PCR." Clinical Chemistry 52, no. 11 (2006): 1997–2004. http://dx.doi.org/10.1373/clinchem.2006.069286.

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Abstract Background: Broad-range PCR provides valuable information for detecting bacterial infections. This study assesses the combined use of broad-range real-time PCR and high-resolution melting analysis for rapid detection and identification of clinically important bacteria. Methods: We subjected 46 bacterial culture colonies representing 25 clinically important bacterial species to LightCycler real-time PCR amplification of the 16S rRNA gene in the presence of LCGreen I fluorescent dye. We performed high-resolution melting analysis of the PCR products with the HR-1 instrument and used melt

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26

Towler, William I., Maria M. James, Stuart C. Ray, et al. "Analysis of HIV Diversity Using a High-Resolution Melting Assay." AIDS Research and Human Retroviruses 26, no. 8 (2010): 913–18. http://dx.doi.org/10.1089/aid.2009.0259.

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27

Bignell, Patricia A., David M. Keeling, and Paul Giangrande. "Detection of F8 Mutations by High Resolution Melting (HRM) Analysis." Blood 112, no. 11 (2008): 1226. http://dx.doi.org/10.1182/blood.v112.11.1226.1226.

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Abstract Approximately 50% of severe and all moderate or mild haemophilia A (HA) mutations are single base substitutions, small insertions or deletions with over 900 different mutations having been described. The usual way to screen these HA cases is by DNA sequencing of all 26 exons which is time consuming and expensive. We have developed the technique of HRM analysis using the Corbett Rotor Gene 6000 for successful high throughput screening of HA mutations. A target sequence was first amplified in the presence of a dsDNA intercalating fluorescent dye followed by in-tube melting analysis. Ini

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28

DANG, XIAO‐DONG, COLIN T. KELLEHER, EMMA HOWARD‐WILLIAMS, and CONOR V. MEADE. "Rapid identification of chloroplast haplotypes using High Resolution Melting analysis." Molecular Ecology Resources 12, no. 5 (2012): 894–908. http://dx.doi.org/10.1111/j.1755-0998.2012.03164.x.

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29

Langaee, Taimour, Lynda Stauffer, Cheryl Galloway, Mohamed H. Solayman, and Larisa Cavallari. "Cross-Validation of High-Resolution Melting Analysis-Based Genotyping Platform." Genetic Testing and Molecular Biomarkers 21, no. 4 (2017): 259–64. http://dx.doi.org/10.1089/gtmb.2016.0317.

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30

Issa, Rahizan, Hatijah Abdul, Siti Hasmah Hashim, Valentinus H. Seradja, Nurul ‘Aishah Shaili, and Nurul Akma Mohd Hassan. "High resolution melting analysis for the differentiation of Mycobacterium species." Journal of Medical Microbiology 63, no. 10 (2014): 1284–87. http://dx.doi.org/10.1099/jmm.0.072611-0.

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A quantitative real-time PCR (qPCR) followed by high resolution melting (HRM) analysis was developed for the differentiation of Mycobacterium species. Rapid differentiation of Mycobacterium species is necessary for the effective diagnosis and management of tuberculosis. In this study, the 16S rRNA gene was tested as the target since this has been identified as a suitable target for the identification of mycobacteria species. During the temperature gradient and primer optimization process, the melting peak (Tm) analysis was determined at a concentration of 50 ng DNA template and 0.3, 0.4 and 0.

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31

Everman, Steven, and Shiao Y. Wang. "Rapid differentiation of bacterial communities using high resolution melting analysis." Journal of Microbiological Methods 140 (September 2017): 77–81. http://dx.doi.org/10.1016/j.mimet.2017.07.006.

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32

Kim, Jaai, and Changsoo Lee. "Rapid fingerprinting of methanogenic communities by high-resolution melting analysis." Bioresource Technology 174 (December 2014): 321–27. http://dx.doi.org/10.1016/j.biortech.2014.10.037.

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33

Li, Jian-Hong, Yue-Ping Yin, He-Ping Zheng, et al. "A high-resolution melting analysis for genotyping urogenital Chlamydia trachomatis." Diagnostic Microbiology and Infectious Disease 68, no. 4 (2010): 366–74. http://dx.doi.org/10.1016/j.diagmicrobio.2010.07.013.

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34

Vaughn, Cecily P., and Kojo S. J. Elenitoba-Johnson. "High-Resolution Melting Analysis for Detection of Internal Tandem Duplications." Journal of Molecular Diagnostics 6, no. 3 (2004): 211–16. http://dx.doi.org/10.1016/s1525-1578(10)60512-0.

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35

Montgomery, Jesse L., Lindsay N. Sanford, and Carl T. Wittwer. "High-resolution DNA melting analysis in clinical research and diagnostics." Expert Review of Molecular Diagnostics 10, no. 2 (2010): 219–40. http://dx.doi.org/10.1586/erm.09.84.

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36

Chang, Chun-Chi, Pei-Chin Lin, Ching-Hsiung Lin, Kun-Tu Yeh, Hsiao-Yu Hung, and Jan-Gowth Chang. "Rapid identification of CYP2C8 polymorphisms by high resolution melting analysis." Clinica Chimica Acta 413, no. 1-2 (2012): 298–302. http://dx.doi.org/10.1016/j.cca.2011.10.005.

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37

Wu, Shu-Biao, Michelle G. Wirthensohn, Peter Hunt, John P. Gibson, and Margaret Sedgley. "High resolution melting analysis of almond SNPs derived from ESTs." Theoretical and Applied Genetics 118, no. 1 (2008): 1–14. http://dx.doi.org/10.1007/s00122-008-0870-8.

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38

Funayama, Manabu, Hiroyuki Tomiyama, Ruey-Meei Wu, et al. "Rapid screening of ATP13A2 variant with high-resolution melting analysis." Movement Disorders 25, no. 14 (2010): 2434–37. http://dx.doi.org/10.1002/mds.23106.

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39

Yimniam, Walaiporn, та Sumalee Jindadamrongwech. "Scanning for α-Hemoglobin Variants by High-Resolution Melting Analysis". Journal of Clinical Laboratory Analysis 30, № 5 (2016): 633–40. http://dx.doi.org/10.1002/jcla.21914.

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40

Shi, Minglan, Xiqing Li, Jiao Feng, et al. "High-resolution melting analysis assay for identification of Fonsecaea species." Journal of Clinical Laboratory Analysis 32, no. 2 (2017): e22257. http://dx.doi.org/10.1002/jcla.22257.

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41

Tungphatthong, Chayapol, Jutharat Somnuek, Thatree Phadungcharoen, Kornkanok Ingkaninan, Jessada Denduangboripant, and Suchada Sukrong. "DNA barcoding of species of Bacopa coupled with high-resolution melting analysis." Genome 61, no. 12 (2018): 867–77. http://dx.doi.org/10.1139/gen-2018-0059.

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In Thailand, there are three species of Bacopa, namely, B. monnieri, B. caroliniana, and B. floribunda. Among these species of Bacopa, B. monnieri is the only medicinal species, used for the treatment of cognitive impairment and improvement of cognitive abilities because of its bioactive constituents, bacoside A and B. However, because of the similar characteristics of these species, it is difficult to differentiate among related species, resulting in confusion during identification. For this reason, and to ensure therapeutic quality for consumers, authentication is important. In this study, t

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42

De Leeneer, Kim, Ilse Coene, Bruce Poppe, Anne De Paepe, and Kathleen Claes. "Rapid and Sensitive Detection of BRCA1/2 Mutations in a Diagnostic Setting: Comparison of Two High-Resolution Melting Platforms." Clinical Chemistry 54, no. 6 (2008): 982–89. http://dx.doi.org/10.1373/clinchem.2007.098764.

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Abstract Background: High-resolution melting is an emerging technique for detection of nucleic acid sequence variations. Developments in instrumentation and saturating intercalating dyes have made accurate high-resolution melting analysis possible and created opportunities to use this technology in diagnostic settings. We evaluated 2 high-resolution melting instruments for screening BRCA1 and BRCA2 mutations. Methods: To cover the complete coding region and splice sites, we designed 112 PCR amplicons (136–435 bp), amplifiable with a single PCR program. LCGreen® Plus was used as the intercalati

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43

HARA, Masayuki, Kazuyoshi YANO, and Etsuko UTAGAWA. "Rapid High-throughput Development on High-resolution Melting (HRM) Analysis for Noroviruses." Kansenshogaku Zasshi 84, no. 3 (2010): 315–16. http://dx.doi.org/10.11150/kansenshogakuzasshi.84.315.

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44

Ney, Jasmin Teresa, Stefanie Froehner, Angelika Roesler, Reinhard Buettner, and Sabine Merkelbach-Bruse. "High-Resolution Melting Analysis as a Sensitive Prescreening Diagnostic Tool to Detect KRAS, BRAF, PIK3CA, and AKT1 Mutations in Formalin-Fixed, Paraffin-Embedded Tissues." Archives of Pathology & Laboratory Medicine 136, no. 9 (2012): 983–92. http://dx.doi.org/10.5858/arpa.2011-0176-oa.

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Context.—As the availability of targeted therapies for several tumor types increases, the need for rapid and sensitive mutation screening is growing. KRAS mutations constitutively activate the RAS/RAF/mitogen-activated protein kinase (MAPK) pathway and therefore play an important role in anti–epidermal growth factor receptor therapy for patients with colorectal cancers. Mutationally activated PIK3CA and AKT1 genes are promising therapeutic targets in breast cancer. In 60% to 70% of malignant melanomas, a mutation in BRAF can be found. Thus, the blocking of the oncogenic signaling induced by th

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45

Kennerson, Marina L., Trent Warburton, Eva Nelis, et al. "Mutation Scanning the GJB1 Gene with High-Resolution Melting Analysis: Implications for Mutation Scanning of Genes for Charcot-Marie-Tooth Disease." Clinical Chemistry 53, no. 2 (2007): 349–52. http://dx.doi.org/10.1373/clinchem.2006.080010.

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Abstract Background: X-linked Charcot-Marie-Tooth type 1 disease has been associated with 280 mutations in the GJB1 [gap junction protein, beta 1, 32kDa (connexin 32, Charcot-Marie-Tooth neuropathy, X-linked)] gene. High-resolution melting analysis with an automated instrument can be used to scan DNA for alterations, but its use in X-linked disorders has not been described. Methods: A 96-well LightScanner for high resolution melting analysis was used to scan amplicons of the GJB1 gene. All mutations reported in this study had been confirmed previously by sequence analysis. DNA samples were amp

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46

Li, Mei, Luming Zhou, Robert A. Palais, and Carl T. Wittwer. "Genotyping Accuracy of High-Resolution DNA Melting Instruments." Clinical Chemistry 60, no. 6 (2014): 864–72. http://dx.doi.org/10.1373/clinchem.2013.220160.

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Abstract BACKGROUND High-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assessed. METHODS Each genotype of the single nucleotide variant (SNV) (c.3405–29A&gt;T) of CPS1 (carbamoyl-phosphate synthase 1, mitochondrial) was amplified by PCR in the presence of LCGreen Plus with 4 PCR product lengths. After blinding and genotype randomization, samples were m

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47

Montgomery, Jesse, Carl T. Wittwer, Jana O. Kent, and Luming Zhou. "Scanning the Cystic Fibrosis Transmembrane Conductance Regulator Gene Using High-Resolution DNA Melting Analysis." Clinical Chemistry 53, no. 11 (2007): 1891–98. http://dx.doi.org/10.1373/clinchem.2007.092361.

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Abstract Background: Complete gene analysis of the cystic fibrosis transmembrane conductance regulator gene (CFTR) by scanning and/or sequencing is seldom performed because of the cost, time, and labor involved. High-resolution DNA melting analysis is a rapid, closed-tube alternative for gene scanning and genotyping. Methods: The 27 exons of CFTR were amplified in 37 PCR products under identical conditions. Common variants in 96 blood donors were identified in each exon by high-resolution melting on a LightScanner®. We then performed a subsequent blinded study on 30 samples enriched for diseas

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48

Ng, Jacklyn W. S., Deborah C. Holt, Patiyan Andersson, and Philip M. Giffard. "DNA Concentration Can Specify DNA Melting Point in a High-Resolution Melting Analysis Master Mix." Clinical Chemistry 60, no. 2 (2014): 414–16. http://dx.doi.org/10.1373/clinchem.2013.215582.

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49

Reed, Gudrun H., Jana O. Kent, and Carl T. Wittwer. "High-resolution DNA melting analysis for simple and efficient molecular diagnostics." Pharmacogenomics 8, no. 6 (2007): 597–608. http://dx.doi.org/10.2217/14622416.8.6.597.

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50

Zhou, L., J. Vandersteen, L. Wang, et al. "High-resolution DNA melting curve analysis to establish HLA genotypic identity." Tissue Antigens 64, no. 2 (2004): 156–64. http://dx.doi.org/10.1111/j.1399-0039.2004.00248.x.

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