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Dissertations / Theses on the topic 'High-throughput drug screening'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 March 2023

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1

Mavridis, Lazaros. "High throughput virtual drug screening using spherical harmonic molecular surface representations." Thesis, Available from the University of Aberdeen Library and Historic Collections Digital Resources, 2009. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=25936.

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2

Harjes, Daniel I. "High throughput optical sensor arrays for drug screening." Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/38270.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006.<br>Vita. Page 131 blank.<br>Includes bibliographical references (p. 127-130).<br>In the world of drug discovery, high throughput whole cell assays are a critical step in discovering therapeutically relevant drug compounds [1]. This report details the development of several novel sensor systems capable of detecting cellular ion flux in multi-well plate format. Optodes are employed as the primary sensors, which are an optically based ion selective polymer. These assays utilize both potassium and sodium s

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3

Wang, Yuanyuan (Marcia). "Statistical Methods for High Throughput Screening Drug Discovery Data." Thesis, University of Waterloo, 2005. http://hdl.handle.net/10012/1204.

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High Throughput Screening (HTS) is used in drug discovery to screen large numbers of compounds against a biological target. Data on activity against the target are collected for a representative sample of compounds selected from a large library. The goal of drug discovery is to relate the activity of a compound to its chemical structure, which is quantified by various explanatory variables, and hence to identify further active compounds. Often, this application has a very unbalanced class distribution, with a rare active class. <br /><br /> Classification methods are commonly pr

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4

Koutsoukas, Alexios. "Virtual screening and bioactivities of small molecules." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708215.

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5

Kanvatirth, Panchali. "Deconvolution of Mycobacterium tuberculosis drug targets using high throughput screening approaches." Thesis, University of Birmingham, 2018. http://etheses.bham.ac.uk//id/eprint/8294/.

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Tuberculosis (TB) is an infectious bacterial disease mainly infecting the pulmonary system of the human body. It affects around 1.5 million people every year, most of whom live in developing countries. The incidence of TB has increased in line with the rise in incidences of Human Immunodeficiency Virus (HIV) infections and Acquired immune deficiency syndrome (AIDS). Due to the pressing concerns of TB, the World Health Organisation (WHO) came up with the Direct Observed Treatment (DOTS) programme. Unfortunately, the development of several resistant strains against first-line drugs and consequen

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Evans, Matthew Darold. "Drug candidate discovery by high-throughput virtual screening of protein binding sites /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2006. http://uclibs.org/PID/11984.

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7

Tian, Honglei. "A high throughput screening method for anti-cancer drug leads discovery from the herbal medicine /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BIEN%202006%20TIAN.

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8

Gage, Zoe O. "Interferon, viruses and drug discovery." Thesis, University of St Andrews, 2017. http://hdl.handle.net/10023/10127.

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The interferon (IFN) response is a crucial component of cellular innate immunity, vital for controlling virus infections. Dysregulation of the IFN response however can lead to serious medical conditions including autoimmune disorders. Modulators of IFN induction and signalling could be used to treat these diseases and as tools to further understand the IFN response and viral infections. We have developed cell-based assays to identify modulators of IFN induction and signalling, based on A549 cell lines where a GFP gene is under the control of the IFN-β promoter (A549/pr(IFN-β).GFP) and the ISRE

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9

Bock, Jonathan Ely. "High throughput screening for drug interactions in fungi and analysis through metabolic profiling /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2009. http://uclibs.org/PID/11984.

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10

Fryknäs, Mårten. "Molecular Screening for Target Discovery in Cancer." Doctoral thesis, Uppsala universitet, Institutionen för genetik och patologi, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7086.

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Cancer is one of the major causes of death in the western world. Resistance to anti-cancer drugs and diagnostic difficulties are major obstacles to successful treatment. This thesis describes studies based on microarray expression analysis and high-throughput compound screening for identification of resistance mechanisms, drug targets and diagnostic markers. In paper I-IV, we applied global expression analysis and measurements of drug response in a human tumor cell line panel to identify drug targets and resistance mechanisms. In paper I, we identified gene transcript levels that correlate wit

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11

Mehta, Kalpita Deepak. "Commercialization of Transiently Transfected Cell Lines for High Throughput Drug Screening and Profiling Applications." Case Western Reserve University School of Graduate Studies / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1269628794.

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12

Natarajan, Anupama. "Patterned cell cultures for high throughput studies of cell electrophysiology and drug screening applications." Doctoral diss., University of Central Florida, 2010. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/4626.

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In conclusion, this study demonstrates the development and testing of in vitro high-throughput systems that have applications in drug development, understanding disease models and tissue engineering. It can be further developed for use with human cells to have a more predictive value than existing complex, expensive and time consuming methods.; Our results, using our simple test system, are in agreement with earlier observations that utilized a complex 3D biodegradable scaffold. Thus, surface functionalization with self-assembled monolayers combined with histological/physiological testing coul

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13

Wu, Yuelong Ph D. Massachusetts Institute of Technology. "A high-throughput antiepileptic drug screening system based on chemically Induced zebrafish behavioral model." Thesis, Massachusetts Institute of Technology, 2014. http://hdl.handle.net/1721.1/93816.

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Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2014.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 53-59).<br>Epilepsy, which has the largest worldwide impacts among all nervous system diseases expect for stroke and dementia, is a group of long-term neurological disorders characterized by epileptic seizures. AED medications are the mainstay for epileptic seizure management. However, the existing AEDs cannot fit the needs for every patient due to the efficacy and side effect issues. In this thesis, a high-throu

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14

Zhang, Xudong. "3-D cell-based high-throughput screening for drug discovery and cell culture process development." Columbus, Ohio : Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1204701561.

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15

Bapat, Aditi Ajit. "Inhibition of Ape1's DNA repair activity as a target in cancer identification of novel small molecules that have translational potential for molecularly targeted cancer therapy /." Connect to resource online, 2009. http://hdl.handle.net/1805/2062.

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Thesis (Ph.D.)--Indiana University, 2009.<br>Title from screen (viewed on February 2, 2010). Department of Biochemistry and Molecular Biology, Indiana University-Purdue University Indianapolis (IUPUI). Advisor(s): Mark R. Kelley, Millie M. Georgiadis, John J. Turchi, Martin L. Smith. Includes vitae. Includes bibliographical references (leaves 114-133).

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16

Barker, Samuel Peter. "Early stage drug discovery screening for novel compounds active against the persister phenotype in Burkholderia thailandensis." Thesis, University of Exeter, 2016. http://hdl.handle.net/10871/26236.

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Many pathogenic microorganisms are believed to stochastically switch into low metabolic states that display resistance to supra-lethal levels of antibiotics. These so-called “persister” cells have been associated with recurrent infections and the development of antibiotic resistance. Whilst a compound that eliminates Staphylococcus aureus persister cells has been described, it is not active against Gram-negative bacteria. The aim of my PhD project was to develop a high-throughput assay for compounds that eradicate persister cells in the -proteobacterium Burkholderia thailandensis. Further to

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17

Kenney, Shelby R. "Development of a high throughput small molecule screen using Staphylococcus aureus invasion of cells." Muncie, Ind. : Ball State University, 2009. http://cardinalscholar.bsu.edu/404.

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18

Suh, Caitlin D. "The Use of High-Throughput Virtual Screening Software in the Proposal of A Novel Treatment for Congenital Heart Defects." Scholarship @ Claremont, 2019. https://scholarship.claremont.edu/cmc_theses/2260.

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Conventional screening of potential drug candidates through wet lab affinity experiments using libraries of thousands of modified molecules is time and resource consuming, along with the fact that it contributes to the widening time gap between the discovery of disease-causing mutations and the implementation of resulting novel treatments. It is necessary to explore whether the preliminary use of high-throughput virtual screening (HTVS) software such as PyRx will curb both the time and money spent in discovering novel treatments for diseases such as congenital heart defects (CHDs). For example

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19

Krause, Michael Allen. "Investigation of genetic determinants of drug response in a Plasmodium falciparum genetic cross using a high-throughput screening method." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:d11eb018-c23a-4c67-97de-f724a39d5df4.

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The emergence and rapid spread of artemisinin-resistant Plasmodium falciparum in southeast Asia highlights the importance of identifying genetic determinants of drug response and discovering novel potent antimalarials. In support of these efforts, the parental lines and progeny of the 803xGB4 genetic cross were characterized by pharmaceutical compound screening and whole-genome sequencing (WGS). Results of the compound screen identified 52 highly-active compounds with potential for development as antimalarials. A confirmatory screen was performed to more accurately define parasite responses to

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20

Laryea, Daniel. "Preclinical Characterization in vivo and in vitro of Novel Agents for Cancer Chemotherapy : Studies on Benomyl, Carbendazim, Cryptolepine and Acriflavine." Doctoral thesis, Uppsala universitet, Klinisk farmakologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-130330.

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Preclinical methods for the identification and characterization of molecules for development into new cancer drugs were investigated. Based on repurposing, i.e. the exploration of currently prescribed drugs for new indications, and as a result of a new high throughput screening (HTS) approach, the benzimidazoles benomyl and carbendazim, the alkaloid cryptolepine and the acridine acriflavine were found interesting to characterize using these methods. In mice the benzimidazoles inhibited 3H-thymidine incorporation in tissues with high cell renewal, with benomyl being more active than carbendazim

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21

Duong-Thi, Minh-Dao. "Introducing weak affinity chromatography to drug discovery with focus on fragment screening." Doctoral thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-24642.

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Fragment-based drug discovery is an emerging process that has gained popularity in recent years. The process starts from small molecules called fragments. One major step in fragment-based drug discovery is fragment screening, which is a strategy to screen libraries of small molecules to find hits. The strategy in theory is more efficient than traditional high-throughput screening that works with larger molecules. As fragments intrinsically possess weak affinity to a target, detection techniques of high sensitivity to affinity are required for fragment screening. Furthermore, the use of differe

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22

Scaduto, Ryan. "A Natural Product and High-Throughput Screening Synthetic Approach Towards the Discovery of Antileishmanial Agents." Ohio Dominican University Honors Theses / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=oduhonors1620081955401195.

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23

He, Shanshan. "Neglected Tropical Disease Chemotherapy: Mechanistic Characterization of Antitrypanosomal Dihydroquinolines and Development of a High Throughput Antileishmanial Screening Assay." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1337980540.

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24

Tiwana, Gaganpreet Singh. "Discovery and investigation of novel radiosensitising genes." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:ee44297c-9b01-4c31-a4f8-6be3585c3557.

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Radiotherapy is second only to surgery in the curative management of patients with cancer, and yet the molecular mechanisms that determine the sensitivity of tumours to radiation remain largely unclear. A high-throughput radiosensitivity screening method based on clonogenicity was developed and a siRNA library against kinase targets was screened. The gold standard colony formation endpoint was chosen for determining reproductive cell death after radiation treatment, since effects on proliferation often do not reflect survival. Thiamine pyrophosphokinase-1 (TPK1), a key component of Vitamin B1/

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25

Lee, Bill. "Preclinical antimicrobial drug discovery : development and evaluation of a platform for high-throughput screening in vitro and an immunocompromised animal model." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100745.

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The incidence of infections caused by antibiotic-resistant bacteria and fungi is rising rapidly. Once considered as little more than a nuisance, antibiotic resistance has become a serious threat. The mortality rate for some infections is approaching that of the pre-antibiotic era. New antimicrobials are needed urgently. Prior to the introduction of any new antimicrobial, comprehensive toxicity and efficacy profiles are assessed in preclinical studies. This thesis focuses on two key stages of preclinical antimicrobial drug development, specifically compound screening in vitro and animal efficac

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26

Senkowski, Wojciech. "High-throughput screening using multicellular tumor spheroids to reveal and exploit tumor-specific vulnerabilities." Doctoral thesis, Uppsala universitet, Cancerfarmakologi och beräkningsmedicin, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-320598.

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High-throughput drug screening (HTS) in live cells is often a vital part of the preclinical anticancer drug discovery process. So far, two-dimensional (2D) monolayer cell cultures have been the most prevalent model in HTS endeavors. However, 2D cell cultures often fail to recapitulate the complex microenvironments of in vivo tumors. Monolayer cultures are highly proliferative and generally do not contain quiescent cells, thought to be one of the main reasons for the anticancer therapy failure in clinic. Thus, there is a need for in vitro cellular models that would increase predictive value of

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Mohammad, Jiyan Mageed. "Therapeutic Potential of Piperlongumine for Pancreatic Ductal Adenocarcinoma." Diss., North Dakota State University, 2019. https://hdl.handle.net/10365/31347.

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Pancreatic ductal adenocarcinoma (PDAC) is among the most lethal malignancies because it is often diagnosed at a late disease stage and has a poor response rate to currently available treatments. Therefore, it is critical to develop new therapeutic approaches that will enhance the efficacy and reduce the toxicity of currently used therapies. Here we aimed to evaluate the therapeutic potential and mechanisms of action for piperlongumine (PL), an alkaloid from long pepper, in PDAC models. We postulated that PL causes PDAC cell death through oxidative stress and complements the therapeutic effica

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Ashman, Stephen M. "An examination of novel fluorescent assay methodologies for proteases, compatible with miniaturised high throughput screening and drug discovery : caspase-3, a case study." Thesis, University of Bristol, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288311.

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29

Yakovich, Adam J. "Old targets and new beginnings a multifaceted approach to combating Leishmaniasis, a neglected tropical disease /." Columbus, Ohio : Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1193247442.

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30

van, Oosten Luuk Nico [Verfasser], and Christian D. [Akademischer Betreuer] Klein. "A novel high-throughput and label-free phenotypic drug screening approach: MALDI-TOF mass spectrometry combined with machine learning strategies / Luuk Nico van Oosten ; Betreuer: Christian D. Klein." Heidelberg : Universitätsbibliothek Heidelberg, 2020. http://d-nb.info/1211820904/34.

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CHHABRA, MONICA. "Modeling and Analysis of Ligand Docking to Norovirus Capsid Protein for the Computer-Aided Drug Design." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1209001634.

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32

Chen, Jonathan Jun Feng. "Data Mining/Machine Learning Techniques for Drug Discovery: Computational and Experimental Pipeline Development." University of Akron / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=akron1524661027035591.

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33

Berry, Michael. "Massively-Parallel Computational Identification of Novel Broad Spectrum Antivirals to Combat Coronavirus Infection." University of the Western Cape, 2015. http://hdl.handle.net/11394/8321.

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Philosophiae Doctor - PhD<br>Given the significant disease burden caused by human coronaviruses, the discovery of an effective antiviral strategy is paramount, however there is still no effective therapy to combat infection. This thesis details the in silica exploration of ligand libraries to identify candidate lead compounds that, based on multiple criteria, have a high probability of inhibiting the 3 chymotrypsin-like protease (3CUro) of human coronaviruses. Atomistic models of the 3CUro were obtained from the Protein Data Bank or theoretical models were successfully generated by homology m

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34

Al-Baghdadi, Osamah Basim Khalaf. "Inhibition of monoamine oxidase by derivatives of piperine, an alkaloid from the pepper plant Piper nigrum, for possible use in Parkinson’s disease." Kent State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=kent1413741660.

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35

Wan, Xiao. "Development of advanced three-dimensional tumour models for anti-cancer drug testing." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:5342fe46-c676-4fe8-8b6e-96d17a18d17d.

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Animal testing is still the common method to test the efficacy of new drugs, but tissue engineered in vitro models are becoming more acceptable for replacing and reducing animal testing in anti-cancer drug screening by developing in vitro three-dimensional (3D) tumour models for anti-cancer drug testing. In this study, three-dimensional (3D) culture methods were developed to mimic the tumour microenvironment. 3D culturing is to seed, maintain and expand cultured cells in three-dimensional space, in contrast to the traditional two-dimensional (2D) method in which the cells attach to the bottom

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Caballero, de la Torre María 1992. "Targeting the RB pathway in tumor cells." Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2021. http://hdl.handle.net/10803/672642.

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Retinoblastoma protein (RB) is a negative regulator of the cell cycle. In response to environmental stress, p38 MAPK phosphorylates RB in the N-terminal region, increasing the affinity of RB for E2F, repressing gene expression and arresting the cell cycle. Expression of a p38-phosphomimetic RB mutant decreases the proliferation of cancer cells and tumors in mice. In this thesis, we have designed an assay that monitors RB activity in cells to identify compounds that mimic the effect of RB phosphorylation by p38. This assay has been optimized for High Throughput Screening (HTS) and we have teste

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Odhar, Hasanain. "Identification of novel scaffolds for Monoamine oxidase B inhibitors." Kent State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=kent1394416913.

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Adanja, Ivan. "Automated tracking of unmarked cells migrating in three-dimensional matrices." Doctoral thesis, Universite Libre de Bruxelles, 2012. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209703.

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The goal of this thesis is the development of a tracking algorithm for populations of unmarked cancer cells that migrate in 3D in vitro gels. The tracking algorithm is intended to be a tool for analysing the motility of large population (i.e. hundreds) of cells in the context of the anti-migratory drug development and more specifically drug screening. In oncology, cancer cell migration plays pivotal roles in the spread of cancer cells from a primary tumor site to neighboring and secondary sites, i.e. the processes of tissue invasion and metastasis. Preventing such processes represents an impor

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Cardno, Tony Stuart, and n/a. "Development of a high throughput fluorescent screening assay for genetic recoding." University of Otago. Department of Biochemistry, 2007. http://adt.otago.ac.nz./public/adt-NZDU20071218.145806.

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The development of new drug therapies traditionally requires mass screening of thousands if not millions of substances to identify lead compounds. They are then further optimised to increase potency. The screening of the large pharmaceutical compound libraries can be incredibly expensive, with the industry responding by miniaturising the assays to smaller formats, enabling the compound screening to be automated and, importantly, eliminating assay reagents that are a major contributing cost for running large screens. A potential target for such an approach is the genetic recoding site of viru

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Xin, Xin. "Development of 3D Cell-Based Assay for High Throughput Screening of Cancer Drugs." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492700405342723.

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Gianella-Borradori, Matteo Luca. "The identification & optimisation of endogenous signalling pathway modulators." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:4c87de5d-24a7-4998-8edb-917c3922aae1.

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<strong>Chapter 1</strong> Provides an overview of drug discovery with particular emphasis on library selection and hit identification methods using virtual based approaches. <strong>Chapter 2</strong> Gives an outline of the bone morphogenetic protein (BMP) signalling pathway and literature BMP pathway modulators. The association between the regulation of BMP pathway and cardiomyogenesis is also described. <strong>Chapter 3</strong> Describes the use of ligand based virtual screening to discover small molecule activators of the BMP signalling pathway. A robust cell based BMP responsive gene a

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Marques, Pinheiro Alice. "Implication du métabolisme de la sérotonine dans les cancers du sein triple négatifs et perspectives cliniques." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS265.

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Les cancers triple négatif (TN) représente la forme la plus agressive des cancers du sein, avec un pourcentage de décès importants. Il existe une grande hétérogénéité au sein de cette maladie en termes de présentation clinique initiale, de caractéristiques biologiques, de sensibilité au traitement et d’évolution. Aucun progrès en survie n’a été réalisé depuis l’avènement des protocoles de chimiothérapie standards. En effet, malgré une bonne réponse initiale au traitement, 65% des patientes résistent aux thérapies actuelles et récidivent ce qui leur confère un pronostic particulièrement sombre.

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Shcherbakova, Elena G. "Implementation of High Throughput Screening Strategies in Optical Sensing for Pharmaceutical Engineering." Bowling Green State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1510758614142002.

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Avedis, Ani. "A high-throughput method for screening of protein binding behavior of multimodal anionic exchange ligands." Thesis, Uppsala universitet, Analytisk farmaceutisk kemi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-434809.

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The biopharmaceutical industry is constantly developing biological drugs, resulting in increased levels of product related impurities having similar characteristics as the target. The aim of the ligand project was to address future challenging purifications by developing new ligands for future resins for the biopharmaceutical industry. The purpose of this study was to develop a high-throughput screening method and use it to compare 15 novel multimodal anionic exchange ligand analogues with two reference ligands, for future polishing steps in the downstream process. The protein binding behavior

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Guthery, Bill. "The development of high-throughput and high-precision analytical methods for the screening of drugs of abuse in biological matrices." Thesis, Open University, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.504252.

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The qualitative determination of various opiates and benzodiazepines in human serum is described using a two-dimensional gas chromatograph (GC x GC) coupled to a time of flight mass spectrometer (TOFMS). Human serum was 'spiked' with known quantities of benzodiazepines and a 'street heroin' mixture including some of the major metabolites and impurities. The sample components were extracted from the matrix by solid phase extraction (SPE). Constituents containing polar hydroxyl and/or secondary amine groups were derivatised with N-methyl-N-(tertbutyldimethyl) trifluoracetamide (MTBSTFA) to impro

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Skone, Gwyn S. "Stratagems for effective function evaluation in computational chemistry." Thesis, University of Oxford, 2010. http://ora.ox.ac.uk/objects/uuid:8843465b-3e5f-45d9-a973-3b27949407ef.

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In recent years, the potential benefits of high-throughput virtual screening to the drug discovery community have been recognized, bringing an increase in the number of tools developed for this purpose. These programs have to process large quantities of data, searching for an optimal solution in a vast combinatorial range. This is particularly the case for protein-ligand docking, since proteins are sophisticated structures with complicated interactions for which either molecule might reshape itself. Even the very limited flexibility model to be considered here, using ligand conformation ensemb

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Wu, Jia-Hao, and 吳家豪. "High-Throughput Cell-Based Drug Screening for Huntington's Disease Therapeutics." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/h5g9e6.

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碩士<br>國立陽明大學<br>生物藥學研究所<br>103<br>Huntington’s disease is an inherited and neurodegenerative disease causing loss control of their voluntary and involuntary actions of patients day by day. The most common symptoms of Huntington’s disease are intelligence retard and chorea, uncontrollable actions like dancing. Patients died around 10 to 20 years because of the dysphagia and dyspnea finally. To establish an image-based high throughput- screening model for a series of drugs screening tests to discover new candidate drugs. With HEK293T cell line, which has similar patterns of poly-glutamine

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Seo, Min Jeong 1979. "Development of high throughput screening systems for the efficient production of antibody fragments in Escherichia coli." 2008. http://hdl.handle.net/2152/17756.

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Recombinant antibodies and antibody fragments have become powerful tools for therapy, in vivo and in vitro diagnostics, and laboratory research. However, the production of antibody fragments in high yield for preclinical and clinical trials can be a serious bottleneck in drug discovery. This dissertation describes the development of novel screening systems for isolating antibody fragments and alternatively, E. coli genes, that facilitate expression in E. coli. In the first part of this work, we have developed a screening system for isolating Fab mutants exhibiting 4~5 fold higher expression le

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Ray, Forest. "PLATE-Seq: An Efficient and Scalable Method for Using RNA-Seq as a Primary Output in High Throughput Drug Screens." Thesis, 2016. https://doi.org/10.7916/D8DV1K17.

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The identification of drug treatments that are useful in diverse therapeutic settings is a significant driving force in biomedical research [Macarron et al., 2011], [Poureetezadi et al., 2014], [Lamb, 2007]. Typical means for measuring the efficacy of a drug for a given clinical application include protein-protein interactions, cell death, mitochondrial respiration and cell growth as well as broader measurements of absorption, distribution, metabolism, excretion and toxicity (ADMET), specifically related the the drug or drugs being tested [Szakcs et al., 2008]. A wide array of methods are rout

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Yozwiak, Carrie Elizabeth. "Development of Methods for the Discovery of Small Molecule Biological Probes." Thesis, 2017. https://doi.org/10.7916/D8NZ8KW9.

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Abstract:

Advances in combinatorial chemistry have facilitated the production of large chemical libraries that can be used as tools to discover biological probes and therapeutics. High-throughput screening (HTS) strategies have emerged as the standard method to assess the biological activity of small molecules. These screens involve the individual analysis of each small molecule in multi-well plates, often requiring expensive automated methods and development of robust assays that may not translate to physiologically relevant contexts. This problem of evaluating large numbers of reagents in phy

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